In this task, you're given a paragraph from the research paper and your task is to generate a suitable title for the research paper based on the given paper. Under 100 words is a good title length.

In this review, we report that the receptor of mouse hepatitis virus (MHV), carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1), is an important determinant of mouse susceptibility to MHV infection. This finding was revealed by using mouse strains with two different allelic forms of the MHV receptor, Ceacam1a and Ceacam1b. Although previous studies indicated that susceptibility is determined by a single gene, Ceacam1, our recent work in gene-replaced mice with chimeric Ceacam1 pointed toward the involvement of other host factors (genes) in the susceptibility. Studies on mouse susceptibility to MHV, as well as the factors involved in their susceptibility, are overviewed.
MINI REVIEW ARTICLE Mouse hepatitis virus receptor as a determinant of the mouse susceptibility to MHV infection

Background: Prevention of infection with airborne pathogens and exposure to airborne particulates and aerosols (environmental pollutants and allergens) can be facilitated through use of disposable face masks. The effectiveness of such masks for excluding pathogens and pollutants is dependent on the intrinsic ability of the masks to resist penetration by airborne contaminants. This study evaluated the relative contributions of a mask, valve, and Micro Ventilator on aerosol filtration efficiency of a new N95 respiratory face mask. Methods: The test mask was challenged, using standardized methods, with influenza A and rhinovirus type 14, bacteriophage 174, Staphylococcus aureus (S. aureus), and model pollutants. The statistical significance of results obtained for different challenge microbial agents and for different mask configurations (masks with operational or nonoperational ventilation fans and masks with sealed Smart Valves) was assessed. Results: The results demonstrate >99.7% efficiency of each test mask configuration for exclusion of influenza A virus, rhinovirus 14, and S. aureus and >99.3% efficiency for paraffin oil and sodium chloride (surrogates for PM 2.5 ). Statistically significant differences in effectiveness of the different mask configurations were not identified. The efficiencies of the masks for excluding smaller-size (i.e., rhinovirus and bacteriophage 174) vs. larger-size microbial agents (influenza virus, S. aureus) were not significantly different. Conclusions: The masks, with or without features intended for enhancing comfort, provide protection against both small-and large-size pathogens. Importantly, the mask appears to be highly efficient for filtration of pathogens, including influenza and rhinoviruses, as well as the fine particulates (PM 2.5 ) present in aerosols that represent a greater challenge for many types of dental and surgical masks. This renders this individual-use N95 respiratory mask an improvement over the former types of masks for protection against a variety of environmental contaminants including PM 2.5 and pathogens such as influenza and rhinoviruses.
Assessment of a respiratory face mask for capturing air pollutants and pathogens including human influenza and rhinoviruses

We describe a new method for affinity purification of recombinant proteins using a dual protease protocol. Escherichia coli maltose binding protein (MBP) is employed as an N-terminal tag to increase the yield and solubility of its fusion partners. The MBP moiety is then removed by rhinovirus 3C protease, prior to purification, to yield an N-terminally His 6 -tagged protein. Proteins that are only temporarily rendered soluble by fusing them to MBP are readily identified at this stage because they will precipitate after the MBP tag is removed by 3C protease. The remaining soluble His 6 -tagged protein, if any, is subsequently purified by immobilized metal affinity chromatography (IMAC). Finally, the N-terminal His 6 tag is removed by His 6 -tagged tobacco etch virus (TEV) protease to yield the native recombinant protein, and the His 6 -tagged contaminants are removed by adsorption during a second round of IMAC, leaving only the untagged recombinant protein in the column effluent. The generic strategy described here saves time and effort by removing insoluble aggregates at an early stage in the process while also reducing the tendency of MBP to "stick" to its fusion partners during affinity purification. Published by Elsevier Inc. Abbreviations used: MBP, maltose-binding protein; IMAC, immobilized metal ion affinity chromatography; TEV, tobacco etch virus; PCR, polymerase chain reaction; ORF, open reading frame; ChikV, Chikungunya virus; DHFR, dihydrofolate reductase; DUSP14, dual-specificity phosphatase 14; MERS-CoV 3CL pro C148A, Middle East respiratory syndrome coronavirus 3C-like protease; GFP, green fluorescent protein; RBS, ribosome-binding site; IPTG, isopropyl b-D-1thiogalactopyranoside; SDSePAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis; TVMV, tobacco vein mottling virus.
A dual protease approach for expression and affinity purification of recombinant proteins