In this task, you're given a paragraph from the research paper and your task is to generate a suitable title for the research paper based on the given paper. Under 100 words is a good title length.

Input: Consider Input: BALB/c male mice were mated with either Swiss-Webster or MF1 females to produce first generation cross-bred offspring. Hybridoma cell lines, from the fusion of P3-NS1-Ag4/1 myeloma cells with spleen cells sensitised to the porcine coronavirus causing transmissible gastroenteritis, were injected intraperitoneally into these mice to produce ascitic fluid containing monoclonal antibodies. Mice of 11 weeks of age weighing between 26 and 34 g were used. The volume of ascites produced by mice injected with four of the five hybrid cell lines tested was greater in the cross-bred offspring than in the BALB/c parent. The fifth cell line gave comparable volumes in the MF1 cross-breed and BALB/c parent but a lesser volume in the Swiss-Webster cross-breed. The antibody titres of the ascites as determined by virus neutralisation, radioimmune and indirect immune fluorescence assays, did not differ significantly between mouse types. The ability to use all offspring from a litter of cross-bred mice, irrespective of sex, and the increased volume of ascitic fluid formed in each mouse, permits fewer animals to be used for the production of ascites in these strains, thereby offering considerable economic and ethical advantages over the use of BALB/c mice.

Output: Comparison of ascites production for monoclonal antibodies in BALB/c and BALB/c-derived cross-bred mice

Input: Consider Input: Gene expression studies require appropriate normalization methods for proper evaluation of reference genes. To date, not many studies have been reported on the identification of suitable reference genes in buffaloes. The present study was undertaken to determine the panel of suitable reference genes in heat-stressed buffalo mammary epithelial cells (MECs). Briefly, MEC culture from buffalo mammary gland was exposed to 42 C for one hour and subsequently allowed to recover at 37 C for different time intervals (from 30 m to 48 h). Three different algorithms, geNorm, NormFinder, and BestKeeper softwares, were used to evaluate the stability of 16 potential reference genes from different functional classes. Our data identified RPL4, EEF1A1, and RPS23 genes to be the most appropriate reference genes that could be utilized for normalization of qPCR data in heat-stressed buffalo MECs.

Output: Identification of Appropriate Reference Genes for qRT-PCR Analysis of Heat-Stressed Mammary Epithelial Cells in Riverine Buffaloes (Bubalus bubalis)

Input: Consider Input: Searches related to COVID-19 and face masks in Taiwan increased rapidly, following the announcements of Taiwan' first imported case and reached its peak as local cases were reported. Searches for handwashing were gradually increased in period of face masks shortage in Taiwan. Google Trends provides information on the most common knowledge needed by users and location of searches. In response to the ongoing outbreak, our results demonstrated that Google Trends could potentially define the proper timing and location for practicing appropriate risk communication strategies to the affected population. Objective: An emerging outbreak of COVID-19 has been detected in at least 26 countries worldwide. Given this pandemic situation, robust risk communication is urgently needed particularly in affected countries. Therefore, this study explored the potential use of Google Trends (GT) to monitor public restlessness toward COVID-19 epidemic infection in Taiwan. We retrieved GT data for the specific locations of Taiwan nationwide and subregions using defined search terms related to coronavirus, handwashing, and face masks. Results: Searches related to COVID-19 and face masks in Taiwan increased rapidly, following the announcements of Taiwan' first imported case and reached its peak as local cases were reported. However, searches for handwashing were gradually increased in period of face masks shortage. Moreover, high to moderate correlations between Google relative search volume (RSV) and COVID-19 cases were found in Taipei (lag-3), New Taipei (lag-2), Taoyuan (lag-2), Tainan (lag-1), Taichung (lag0), and Kaohsiung (lag0). In response to the ongoing outbreak, our results demonstrated that GT could potentially define the proper timing and location for practicing appropriate risk communication strategies to the affected population.
Output: Journal Pre-proof Applications of google search trends for risk communication in infectious disease management: A case study of COVID-19 outbreak in Taiwan Applications of Google Search Trends for Risk Communication in Infectious Disease Management: a Case Study of COVID-19 Outbreak in Taiwan