Q: Put on gloves and tie back long hair to prevent contaminating the bacteria sample you'll be testing. Disinfect a workspace under the fume hood, or in another well-ventilated area. Check that the bunsen burner and microscope are functional before you begin. If the glass slide is dirty, wash it in soapy water to remove grease and dirt. Disinfect the slide with ethanol, glass cleaner, or whichever method is recommended by your laboratory. You can use the Gram stain method to help identify bacteria present in medical samples, or bacterial cultures grown in a petri dish. In order for the Gram stain to be useful, add a thin layer of the sample on the stain. A sample under 24 hours old is recommended, as older bacteria may have damaged cell walls that respond less predictably to gram staining. If using a tissue sample, add 12 drops onto the glass slide. Spread it evenly on the slide to form a thin smear, using the edge of a second sterilized glass slide. Allow it to air dry before continuing. If taking bacteria from a petri dish, sterilize an inoculation loop in a Bunsen burner flame until it glows, then let it cool. Use it to place a drop of sterile water on the slide, then sterilize and cool the loop again before transferring a tiny sample of bacteria and gently stirring into the water. Bacteria in broth should be mixed in a vortexer, then added with an inoculation loop as above, without adding the extra water. If you have a swab sample, roll the swab lightly across the slide. Heat will fix the bacteria to the slide, so they are not as easily rinsed off during the stain. Quickly pass the slide two to three times through a Bunsen burner flame, or heat it on top of an electric slide warmer. Do not overheat, or the samples may become distorted. If using a Bunsen burner, the flame should be a small, blue cone, not a tall orange one. Alternatively, the smear may be fixed by methanol instead, by adding 1-2 drops of methanol onto the dried smear, draining off the excess methanol, and allowing it to air dry. This method minimises damage to host cells, giving a cleaner background. A staining tray is a shallow metal, glass, or plastic dish with a small mesh or wire support running across the top. Place the slide on top of this support, so the liquids you'll be using can drain down into the tray. If you do not have a staining tray, the slide can be placed directly on top of a plastic ice cube tray.
A: Prepare for laboratory work. Sterilize a glass microscope slide. Add the sample to the slide. Heat fix the smear. Position the slide on a staining tray.

Article: Be gentle to avoid damaging the components. A little rattling is normal. If it sounds very loud, or you notice a loose piece moving around inside the tube, then your tube is broken and needs to be replaced. Plug the tube into your amplifier or other machine. Bring along a pencil, chopsticks, or another wooden or plastic implement. Use it to gently tap each tube. All tubes ring, but the bad ones sound louder and may cause the device to squeal. Turn off the machine, then swap the tubes. Turn the knobs on your amplifier or other device to activate each tube. Tap the tubes again as you go and listen for the ringing. A bad tube will sound loud no matter what channel it's on. Swapping the old tube with a new one is also a good test. If the old tube is broken, the new one won't ring as much. Slip an oven mitt over your hand. Hold onto the suspect tube as you use your device, such as by playing a note on the guitar hooked up to the vacuum tube amplifier. You will notice much less rattling if the tube is broken.
Question: What is a summary of what this article is about?
Shake the tube for signs of rattling. Tap the tubes with a pencil to listen to their ringing. Swap the tubes to make sure you found the broken one. Hold the tube still while using the device to test it.

Problem: Article: Exercise regularly, just before a stressful situation, or as a response to anger. Research has shown that exercise tells your body to release helpful endorphins that reduce your body's stress response and elevate your mood. All of these can help you calm down. Exercise of any kind can have the same helpful effects on anger, so do an activity you typically enjoy (such as running, dancing, or walking). For example, if you know you have a stressful business meeting later in the day, take some time in the morning to go for a run. This will reduce stress and tension, so you are not walking into your meeting wound up. Although it sounds odd, counting can actually help to slow you down when you are tempted to explode or have a tantrum. Focus your thoughts away from the situation and on to your counting and breathing. Count to 10, taking a breath with each count and in between each number. Doing this at a slow pace can help you slow down and become calmer. Continue to count if you still feel angry after reaching 10. When you're angry, your body releases adrenaline which increases your heart rate, makes you feel flushed, and causes other physical signs of anger or arousal. Counting gives you a chance to slow down and calm your reactions so that you don't act impulsively. Learn to breathe with your diaphragm, the muscle at the lower part of your lungs. Focus on your breathing and take full, deep breaths that fill the entire lung. Breathe in for 5 seconds, then hold for 5 seconds, and release for 5 seconds. Take 2 normal breaths in between each diaphragm breath, so that you do not hyperventilate and repeat the exercise until you feel calmer. Breathing signals your body to calm down. It does this by releasing neurotransmitters that tell your brain that you're ok and safe. When you're angry, adrenaline can increase your breathing rate, making it feel as though breathing is difficult or that you can't get a full breath. Meditation can help you concentrate, reduce your stress levels, and enable self-awareness. All of these things can calm you down when facing an emotional outburst. Try to take at least 10 to 20 minutes a day to practice mindfulness, which can improve your overall mental health. There are several forms of meditation you can try, like Tai Chi, yoga, transcendental meditation, and Qi Gong.
Summary: Exercise. Count. Do breathing exercises. Meditate.

Q: Add 1 cup (267 g) of coarse sea salt to a glass jar or container. Pour in cup (118 ml) of liquid coconut oil, and mix with a fork until the ingredients are fully combined. If you don't have coconut oil, you can substitute olive oil. Once the salt and coconut oil are fully combined, mix in 5 to 7 drops of grapefruit essential oil. Stir the scrub well to distribute the fragrance throughout the mixture. You can use other citrus essential oils if you prefer, such as lemon, lime, or orange. However, grapefruit is particularly good for the scrub because it has antibacterial and antiseptic properties. You can use the scrub once or twice a week. Keep it beside your sink so it's easy to remember to use it when your hands feel dry and rough. Be sure to store the scrub with a lid on to keep it airtight.
A:
Mix the salt and coconut oil. Add the essential oil. Store the scrub beside your sink.