There are several companies that supply laboratories with materials and equipment for conducting cultures. When you purchase the materials, they will also come with protocols for starting the culture. These protocols will describe exactly how you should transfer the sample that you want to culture into the culture environment. Several well-known companies include: Qiagen Fisher Scientific Thermo Scientific It is generally necessary to replenish the culture media every 2 3 days. Follow the manufacturer's protocols for feeding the cells. This process should take 20 minutes or less. If you do not provide sufficient nutrients, the cells that you do have will die. In order to keep the culture growing, you may need to move some of the cells into a new dish with a lower density. This will enable both the original culture and the new one to continue growing. You can also freeze some of the cultures for future work or to repeat experiments if you have problems with contamination in the future. Follow the provided protocols to determine the temperature for cryopreservation or freezing the cultures.
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One-sentence summary -- Follow the manufacturer's protocols for starting the culture. Feed the cells if you are working with mammalian cells. Split cultures that are starting to get crowded.

Q: Factor each denominator digit into a series of prime numbers that multiply together to make that number. Prime numbers are numbers that cannot be divided by any other number. Example: 1/4 + 1/5 + 1/12 Prime factorization of 4: 2 * 2 Prime factorization of 5: 5 Prime factorization of 12: 2 * 2 * 3 Tally up the number of times that each prime number appears in the factorization of each denominator digit. Example: There are two 2's in 4; zero 2's in 5; two 2's in 12 There are zero 3's in 4 and 5; one 3 in 12 There are zero 5's in 4 and 12; one 5 in 5 Identify the largest number of times you used each prime number for any of the denominators and note that count. Example: The largest count of 2 is two; the largest of 3 is one; the largest of 5 is one Do not write out the number of times each prime number appeared throughout all the original denominators. Only write out the largest count, as determined in the previous step. Example: 2, 2, 3, 5 Multiply the prime numbers together as they appeared in the previous step. The product of these numbers equals the LCD for the original equation. Example: 2 * 2 * 3 * 5 = 60 LCD = 60 To determine the multiple needed to make the denominators equal, divide the LCD you determined by the original denominator. Multiply the numerator and the denominator of each fraction by this number. The denominators should now both be equal to the LCD. Example: 60/4 = 15; 60/5 = 12; 60/12 = 5 15 * (1/4) = 15/60; 12 * (1/5) = 12/60; 5 * (1/12) = 5/60 15/60 + 12/60 + 5/60 With the LCD found, you should be able to add and subtract the fractions as usual. Remember to simplify the fraction at the end, if possible. Example: 15/60 + 12/60 + 5/60 = 32/60 = 8/15
A: Break each denominator into prime numbers. Count the number of times each prime appears in each factorization. Take the largest count for each prime. Write that prime as many times as you counted in the previous step. Multiply all the prime numbers written in this manner. Divide the LCD by the original denominator. Solve the rewritten equation.

Article: Curtain rods have the benefit of being easily adjustable while pipes may look more uniform and clean on the wall. Look for rods painted in your team's colors. Grab your jersey by the arms and pull them taut, creating a solid line from the upper tip of one sleeve across the neck hole to the upper tip of the other sleeve. Use a ruler to find the exact length of this line. If you're using a curtain rod, adjust the segments so they are slightly longer than the length of the jersey. If you're using a PVC pipe, use a plastic pipe cutter to achieve the same thing. The rod should be slightly longer than the jersey so you can place the ends on hooks without damaging the clothing. Using a pencil, mark the spot where the center of the jersey's neckline will sit. Then, divide the length of your rod in half. Use a metal ruler to measure out this distance from the left and right of the center point, making a mark at each end. Hold your rod up to the wall to make sure the points are level. To make sure your marks are lined up, place your ruler against the wall so that it intersects with both points. Then, use a level to see if the ruler is straight. If your curtain rod came with specific hanging hardware, follow the included instructions to attach them to the wall. If they did not, or if you're using a PVC pipe, attach a nail hook, screw hook, or thick command strip instead. Make sure the hooks are large enough to hold the rod and thick enough to support its weight. Push your rod through one sleeve of the jersey and run it through to the other side. If necessary, use small tape strips or Glue Dots to keep the shirt from sliding. Place the ends of the rod on your hooks or hanging hardware.
Question: What is a summary of what this article is about?
Purchase a thin curtain rod or PVC pipe. Measure your jersey from sleeve to sleeve. Adjust your rod so it is slightly longer than the jersey. Measure and mark your rod's end-points on the wall. Attach hooks at the marked points. Run your rod through the jersey and hang it.

Article: Stir the water and sugar together in a small saucepan with tall sides. Place the saucepan on a stove over medium-high heat. Start with cold water. The ratio in this recipe will create a thick syrup suitable for cold fruit drinks, cocktails, and candied fruits. To create a syrup with medium thickness for use in iced tea and warm beverages, increase the ratio to two parts water and one part sugar. For a thin syrup suitable for use as a dessert glaze, change the ratio to three parts water and one part sugar. Stir the mixture as it comes to a boil in order to dissolve the sugar. Use medium-high to high heat and stir with a wooden or plastic mixing spoon. It may take 3 to 5 minutes before the mixture reaches a rolling boil. Check to see if the sugar has dissolved by scooping up a small amount in your spoon. If you see sugar crystals, continue to boil the syrup longer. Reduce the heat to low and let it simmer steadily for 10 minutes, stirring occasionally. If you want to create a flavored syrup, add the flavorings while the syrup simmers. Liquid ingredients, like fresh lime or lemon juice, can be added directly to the syrup and stirred in. Solid ingredients, like orange peels, mint springs, or cinnamon sticks, should be tied into a bundle with cheesecloth an twine and steeped in the syrup as it simmers. Remove the syrup from the heat and let it cool to room temperature. Do not place the syrup in the refrigerator during this cooling stage. Instead, let it come to temperature on the counter at room temperature. You can add the syrup to your recipe immediately or pour it into a container to cover and refrigerate for later. The syrup should last in the refrigerator anywhere from one to six months.
Question: What is a summary of what this article is about?
Combine the water and sugar. Bring the mixture to a boil. Reduce the mixture to a simmer. Let cool. Use immediately or save.