In this task, you're given a paragraph and title from the research paper. Your task is to classify whether the given title is suitable or not for the research paper based on the given paragraph. Return "True" if title is proper according to paragraph else "False".

Ex Input:
Paragraph: The yeast Kre2p/Mntlp cxl,2-mannosyltransferase is a type II membrane protein with a short cytoplasmic amino terminus, a membrane-spanning region, and a large catalytic luminal domain containing one N-glycosylation site. Anti-Kre2p/Mntlp antibodies identify a 60-kD integral membrane protein that is progressively N-glycosylated in an MNNl-dependent manner. Kre2p/Mntlp is localized in a Golgi compartment that overlaps with that containing the medial-Golgi mannosyltransferase Mnnlp, and distinct from that including the late Golgi protein Kexlp. To determine which regions of Kre2p/Mntlp are required for Golgi localization, Kre2p/Mntlp mutant proteins were assem-bled by substitution of Kre2p domains with equivalent sequences from the vacuolar proteins DPAP B and Pho8p. Chimeric proteins were tested for correct topology, in vitro and in vivo activity, and were localized intracellularly by indirect immunofluorescence. The resuits demonstrate that the NH2-terminal cytoplasmic domain is necessary for correct Kre2p Golgi localization whereas, the membrane-spanning and stem domains are dispensable. However, in a test of targeting sufficiency, the presence of the entire Kre2p cytoplasmic tail, plus the transmembrane domain and a 36-amino acid residue luminal stem region was required to localize a Pho8p reporter protein to the yeast Golgi. T HE Golgi apparatus plays a fundamental role in glycan processing and sorting of newly synthesized proteins in the secretory pathway of eukaryotic cells. The Golgi apparatus of a typical mammalian cell is composed of a polarized stack of membranous saccules that are differentiated into functionally distinct subcompartments. After the addition of core N-linked sugar moieties in the endoplasmic reticulum, glycoproteins proceed through the cis-Golgi network, the cis-, medial-, and transcisternae, and the trans-Golgi network, where further modifications take place. These include the addition of O-linked sugars and the elaboration, in a protein-specific manner, of complex N-linked carbohydrate structures. The Golgi apparatus also constitutes a major organelle responsible for protein trafficking where particular proteins are directed to precise cellular locations. Two specific Golgi subcompartments have been found to be involved in protein sorting. The cis-Golgi network constitutes the site from which many resident ER proteins are retrieved, and the main function of the TGN is to direct glycoproteins exiting the Golgi complex to either the lysosome or the cell surface (
Title: Localization and Targeting of the Saccharomyces cerevisiae Kre2p/Mnt lp oL 1,2-Mannosyltransferase to a medial.Gol Compartment

Ex Output:
False

Ex Input:
Paragraph: Background: The continuing spread of the newly emerged H7N9 virus among poultry in China, as well as the possibility of human-to-human transmission, has attracted numerous efforts to develop an effective vaccine against H7N9. The use of nanoparticles in vaccinology is inspired by the fact that most pathogens have a dimension within the nano-size range and therefore can be processed efficiently by the immune system, which leads to a potent immune response. Herein, we report a facile approach to increase antigen size to achieve not only fast but also effective responses against the recombinant HA/H7N9 protein via a simple conjugation of the protein onto the surface of nanodiamond particles. In this study, trimeric Haemagglutinin (H7) that is transiently expressed in N. benthamiana was purified using affinity chromatography, and its trimeric state was revealed successfully by the cross-linking reaction. The trimeric H7 solution was subsequently mixed with a nanodiamond suspension in different ratios. The successful conjugation of the trimeric H7 onto the surface of nanodiamond particles was demonstrated by the changes in size and Zetapotential of the particles before and after protein coating, Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and Western-blot analysis. Next, biofunction of the protein-nanodiamond conjugates was screened using a haemagglutination assay. A mixture containing 5 g of trimeric H7 and 60 g of nanodiamond corresponds to a ratio of 1:12 (w/w) of agglutinated chicken red blood cells at HA titer of 1024, which is 512-fold higher than the HA titer of free trimeric H7. After the 2nd and 3rd immunization in mice, ELISA and Western blot analyses demonstrated that the physical mixture of trimeric H7 protein and nanodiamond (1:12, w/w) elicited statistically significant stronger H7-specific-IgG response demonstrated by higher amounts of H7N9-specific IgG (over 15.4-fold with P < 0.05 after the second immunization). These results indicated a potential effect inherent to nanodiamond towards modulating immune systems, which should be further evaluated and broadly applied in nanovaccine development.
Title: Nanodiamond enhances immune responses in mice against recombinant HA/ H7N9 protein

Ex Output:
True

Ex Input:
Paragraph: 166 Y. M. CHABRE AND R. ROY NH 2 HO HO HO HX XH XH HX X = O X = S X = NH Branched aliphatic scaffolds N NH 2 H 2 N NH 2 H 2 N N H NH 2 AB 2 system AB 3 systems Trivalent core Tetravalent core Branched aromatic scaffolds X X X X = COCl, CO 2 H Br, I, CH 2 OH Trivalent core X Y X X = OH, NH 2 , Br, I Y = COOH AB 2 system HO COOH OH HO AB 3 system X = COCl, CO 2 H, Br, I X X Tetravalent cores Cl Cl Cl Cl Cl Cl Hexavalent cores N HN N NH X X O OH HO OH HO OH Hexavalent core Br Br Br Br Br O AcO AcO O AcO OAc OAc O AcO O Br OAc 1) NaN 3 , 15-crown-5 26 R = Bn 27 R = Bz 28 R = H 11 23 24 25 Scheme 4. Synthesis of tetramannosylated pentaerythritol having an extended linker. 75 29 12 32 33 36 Scheme 5. Extended tetramannosylated clusters prepared by Touaibia et al. 76 X O AcO O AcO X = OorS O AcO AcO OAc AcO O AcO AcO AcO OAc O AcO AcO AcO OAc Co 2 (CO) 8 Dioxane, , several days X O AcO X O OAc X O OAc + X O AcO X O OAc X O AcO 66 (Major isomer: 84%) 67 (Major isomer: 16%) , 15-61% 29 64 65 Scheme 9. Cobalt-catalyzed cyclotrimerization of propargylated glycosides. 90 O RO O BzO BzO BzO OBz O AcO AcO AcO OAc O AcO AcO OAc AcO 92 93 94 95 93-95
Title: DESIGN AND CREATIVITY IN SYNTHESIS OF MULTIVALENT NEOGLYCOCONJUGATES

Ex Output:
True