Detailed Instructions: In this task, you're given a paragraph from the research paper and your task is to generate a suitable title for the research paper based on the given paper. Under 100 words is a good title length.
Problem:RNA-RNA recombination salvages viral RNAs and contributes to their genomic variability. A recombinationally-active subgenomic promoter (sgp) has been mapped in Brome mosaic bromovirus (BMV) RNA3 (Wierzchoslawski et al., 2004. J. Virol.78, 8552-8864) and mRNA-like 5 sgRNA3a was characterized (Wierzchoslawski et al., 2006. J. Virol. 80, 12357-12366). In this paper we describe sgRNA3a-mediated recombination in both in vitro and in vivo experiments. BMV replicase-directed co-copying of () RNA3 with wt sgRNA3a generated RNA3 recombinants in vitro, but it failed to when 3-truncated sgRNA3a was substituted, demonstrating a role for the 3 polyA tail. Barley protoplast co-transfections revealed that (i) wt sgRNA3a recombines at the 3 and the internal sites; (ii) 3-truncated sgRNA3as recombine more upstream; and (iii) 5-truncated sgRNA3 recombine at a low rate. In planta co-inoculations confirmed the RNA3-sgRNA3a crossovers. In summary, the non-replicating sgRNA3a recombines with replicating RNA3, most likely via primer extension and/or internal template switching.
Solution:
Recombination of 5 subgenomic RNA3a with genomic RNA3 of Brome mosaic bromovirus in vitro and in vivo