Teacher:In this task, you're given a paragraph and title from the research paper. Your task is to classify whether the given title is suitable or not for the research paper based on the given paragraph. Return "True" if title is proper according to paragraph else "False".
Teacher: Now, understand the problem? Solve this instance: Paragraph: Antibody-based therapeutics is attracting more attention in the post-genome era, in contrast to a diminution in the initial high expectation for rapid development of gene-based therapeutic modalities. In support to the antibody-based therapeutics, the advent of recent technologies has made human antibody screening and production progressively more economic. Among those technologies, phage-display antibody library has been successfully applied in the antibody-based drug development both as fully human antibody sources and tools for antibody engineering. Building up a high-quality antibody library with a large library size and high diversity has been crucial for successful isolation of antibodies. Here we describe an efficient strategy for the construction of a large naive phage-display human Fab library with one-step cloning. Optimization of each key step is extensively discussed and simplified protocols for library panning and Fab production are also described. Phage display; Fab; antibody library; IgM; one-step cloning Building a high-quality phage-displayed antibody library requires a source of large number of well-diversified antibody genes. A variety of immunoglobulin gene sources including blood lymphocytes, bone marrow, and spleen have been used for library construction. From these sources IgM-derived antibody genes have been successfully used for library construction and selection of high-affinity antibodies (4-7), even though IgM-specific antibodies are typically of relatively low affinity in vivo compared to antibodies from the
Title: Construction of a Large Naive Human Phage-Displayed Fab Library Through One-Step Cloning
Student:
True