Detailed Instructions: In this task, you're given a paragraph from the research paper and your task is to generate a suitable title for the research paper based on the given paper. Under 100 words is a good title length.
See one example below:
Problem: The severe acute respiratory syndrome (SARS) epidemic originating from China in 2002 was caused by a previously uncharacterized coronavirus that could be identified by specific RT-PCR amplification. Efforts to control future SARS outbreaks depend on the accurate and early identification of SARS-CoV infected patients. A real-time fluorogenic RT-PCR assay based on the 3 -noncoding region (3 -NCR) of SARS-CoV genome was developed as a quantitative SARS diagnostic tool. The ideal amplification efficiency of a sensitive SARS-CoV RT-PCR assay should yield an E value (PCR product concentration increase per amplification cycle) equal to 2.0. It was demonstrated that the 3 -NCR SARS-CoV based RT-PCR reactions could be formulated to reach excellent E values of 1.81, or 91% amplification efficacy. The SARS-CoV cDNA preparations derived from viral RNA extract and the cloned recombinant plasmid both exhibit the identical amplification characteristics, i.e. amplification efficacy using the same PCR formulation developed in this study. The viral genomic copy (or genomic equivalences, GE) per infectious unit (GE/pfu) of SARS-CoV used in this study was also established to be approximate 1200-1600:1. The assay's detection sensitivity could reach 0.005 pfu or 6-8 GE per assay. It was preliminarily demonstrated that the assay could efficiently detect SARS-CoV from clinical specimens of SARS probable and suspected patients identified in Taiwan. The 3 -NCR based SARS-CoV assay demonstrated 100% diagnostic specificity testing samples of patients with acute respiratory disease from a non-SARS epidemic region.
Solution: Development and evaluation of an efficient 3 -noncoding region-based SARS coronavirus (SARS-CoV) RT-PCR assay for detection of SARS-CoV
Explanation: It's a correct title because the paragraph described the development of 3 -noncoding region of SARS-CoV genome and assay efficiently detecting SARS-CoV from clinical specimens.

Problem: The constituents of Cimicifuga plants have been extensively investigated, and the principal metabolites are 9,19cyclolanostane triterpenoid glycosides, which are distributed widely in Cimicifuga plants, but not in other members of the Ranunculaceae family, and are considered to be characteristics of the Cimicifuga genus. This type of triterpenoid glycoside possesses several important biological activities. More than 120 cycloartane triterpene glycosides have been isolated from Cimicifuga simplex Wormsk. The aim of this review article is to summarize all the major findings based on the available scientific literatures on C. simplex, with a focus on the identified 9,19-cyclolanostane triterpenoid glycosides. Biological studies of cycloartane triterpene glycosides from Cimicifuga spp. are also discussed. Cimicifuga simplex; Ranunculaceae; Cycloartenol triterpenoid saponins; Biological effects [CLC Number] R284, R965 [Document code] A [Article ID] These authors have no any conflict of interest to declare. Published by Elsevier B.V. All rights reserved and biological perspectives. C. simplex, also known as Ku lou ya gen and Long yan gen, is an important species in the original Chinese drug Shengma. In Chinese traditional medicine, as along with other Cimicifuga species, it has been used to clear heat, relieve toxicity, disperse exterior pathogen, promote eruption, and lift spirit [3] . Plant-based formulations for various medicinal applications use different preparation methods, including powders, alcohol extracts, water extracts, and honey processed pills. The roots of C. simplex have been used to treat headache, toothache, aphtha, sore throat, measles, rectocele, and uterine prolapse [11] . The most important biologically active components of C. simplex are the cycloartenol triterpenoid saponins which have good immunosuppressive activities [12] . C. simplex Wormsk. ex DC. (Shengma in Chinese) is a deciduous perennial herb, and is widely distributed in China [13] . Currently, more than 120 cycloartane-type triterpenoids from Cimicifuga simplex have been isolated by several groups [14] [15] [16] [17] [18] [19] [20] [21] [22] [23] [24] [25] [26] [27] , including compounds of cycloartanol type, 16,23-dione type, shengmanol type, hydroshengmanol type, cimifugenin type, cimiacerogenin type, and the cimigenol type. The chemical structures of these cycloartenol triterpenoid saponins are shown in Fig.s 17 and are listed in Table 1 . The isolation scheme WU Lun, et al. / Chin J Nat Med, 2015, 13(2): 8189 -82 -for the 9,19-cyclolanostane triterpenoid glycosides from C. simplex is shown in Fig. 8 .
Solution:
Cycloartenol triterpenoid saponins from Cimicifuga simplex (Ranunculaceae) and their biological effects