Please answer this: Given the following passage "South America became linked to North America through the Isthmus of Panama during the Pliocene, bringing a nearly complete end to South America's distinctive marsupial faunas. The formation of the Isthmus had major consequences on global temperatures, since warm equatorial ocean currents were cut off and an Atlantic cooling cycle began, with cold Arctic and Antarctic waters dropping temperatures in the now-isolated Atlantic Ocean. Africa's collision with Europe formed the Mediterranean Sea, cutting off the remnants of the Tethys Ocean. Sea level changes exposed the land-bridge between Alaska and Asia. Near the end of the Pliocene, about 2.58 million years ago (the start of the Quaternary Period), the current ice age began. The polar regions have since undergone repeated cycles of glaciation and thaw, repeating every 40,000100,000 years.", answer the following question. Note that the answer is present within the text. Question: What happened to the Atlantic during the Pliocene?
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Answer: dropping temperatures

Please answer this: Given the following passage "In the early 20th century, Carl von Voit and Max Rubner independently measured caloric energy expenditure in different species of animals, applying principles of physics in nutrition. In 1906, Edith G. Willcock and Frederick Hopkins showed that the amino acid tryptophan aids the well-being of mice but it did not assure their growth. In the middle of twelve years of attempts to isolate them, Hopkins said in a 1906 lecture that "unsuspected dietetic factors," other than calories, protein, and minerals, are needed to prevent deficiency diseases. In 1907, Stephen M. Babcock and Edwin B. Hart conducted the single-grain experiment, which took nearly four years to complete.", answer the following question. Note that the answer is present within the text. Question: What was proven in the 7th year of the 1900's?
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Answer: Edith G. Willcock and Frederick Hopkins showed that the amino acid tryptophan aids the well-being of mice but it did not assure their growth

Please answer this: Given the following passage "Although Brandenburg was a part of the Holy Roman Empire, the Prussian lands were not within the Holy Roman Empire and were with the administration by the Teutonic Order grandmasters under jurisdiction of the Emperor. In return for supporting Emperor Leopold I in the War of the Spanish Succession, Elector Frederick III was allowed to crown himself "King in Prussia" in 1701. The new kingdom ruled by the Hohenzollern dynasty became known as the Kingdom of Prussia. The designation "Kingdom of Prussia" was gradually applied to the various lands of Brandenburg-Prussia. To differentiate from the larger entity, the former Duchy of Prussia became known as Altpreuen ("Old Prussia"), the province of Prussia, or "East Prussia".", answer the following question. Note that the answer is present within the text. Question: Which of the following is not a person: Leopold, Frederick or Duchy?
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Answer:
Duchy