John celebrates his 40th birthday and later goes skinny dipping with Jenny in their swimming pool. Increasingly disenchanted with his job, he decides to accept a position as a reporter with The Philadelphia Inquirer with Jenny's blessing, and the family moves to a farm in rural Pennsylvania. John soon realizes that he is a better columnist than reporter and pitches the column idea to his editor. Life is idyllic until the aging Marley begins to show signs of arthritis and deafness. An attack of gastric dilatation volvulus almost kills him, but he recovers. When a second attack occurs, it becomes clear surgery will not help him, and Marley is euthanized with John at his side. The family pay their last respects to their beloved pet as they bury him beneath a tree in their front yard.

Can we conclude that does marley die in the movie marley and me?
yes