In this task, you're given a paragraph and title from the research paper. Your task is to classify whether the given title is suitable or not for the research paper based on the given paragraph. Return "True" if title is proper according to paragraph else "False".

Paragraph: Extracts of Anthemis hyalina (Ah), Nigella sativa (Ns) and peels of Citrus sinensis (Cs) have been used as folk medicine to fight antimicrobial diseases. To evaluate the effect of extracts of Ah, Ns and Cs on the replication of coronavirus (CoV) and on the expression of TRP genes during coronavirus infection, HeLa-CEACAM1a (HeLa-epithelial carcinoembryonic antigen-related cell adhesion molecule 1a) cells were inoculated with MHV-A59 (mouse hepatitis virus-A59) at moi of 30. 1/50 dilution of the extracts was found to be the safe active dose. ELISA kits were used to detect the human IL-8 levels. Total RNA was isolated from the infected cells and cDNA was synthesized. Fluidigm Dynamic Array nanofluidic chip 96.96 was used to analyze the mRNA expression of 21 TRP genes and two control genes. Data was analyzed using the BioMark digital array software. Determinations of relative gene expression values were carried out by using the 2 -DDCt method (normalized threshold cycle (Ct) value of sample minus normalized Ct value of control). TCID 50 / ml (tissue culture infectious dose that will produce cytopathic effect in 50 % of the inoculated tissue culture cells) was found for treatments to determine the viral loads. The inflammatory cytokine IL-8 level was found to increase for both 24 and 48 h time points following Ns extract treatment. TRPA1, TRPC4, TRPM6, TRPM7, TRPM8 and TRPV4 were the genes which expression levels changed significantly after Ah, Ns or Cs extract treatments. The virus load decreased when any of the Ah, Ns or Cs extracts was added to the CoV infected cells with Ah extract treatment leading to undetectable virus load for both 6 and 8hpi. Although all the extract treatments had an effect on IL-8 secretion, TRP gene expression and virus load after CoV infection, it was the Ah extract treatment that showed the biggest difference in virus load. Therefore Ah extract is the best candidate in our hands that contains potential treatment molecule(s).
Title: The effects of Nigella sativa (Ns), Anthemis hyalina (Ah) and Citrus sinensis (Cs) extracts on the replication of coronavirus and the expression of TRP genes family
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Paragraph: publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. Abstracts accepted for publication only Pathogenesis R2249 Effect of a probiotic dairy product containing Lactobacillus casei Shirota on the gut microbiota in irritable bowel syndrome: a randomised, placebo-controlled, double-blind study Objectives: It has been hypothesized that disturbances in the intestinal flora could be a factor in the onset and persistence of IBS complaints. The aim of the study was to analyse the effect of Lactobacillus casei Shirota (LcS) on the faecal flora of IBS patients. Methods: In a randomised, placebo controlled, double blind study, IBS patients fulfilling the Rome criteria II were included. Patients took 2 bottles daily for 8 weeks, containing either LcS or placebo. Faecal samples were collected before intervention (week 2/0,S1), at the end of intervention (week 6/8,S2) and during follow-up (week 14/16,S3). Microbial populations (Bacteroides, anaerobes, bifidobacteria, coliforms, clostridia, and lactobacilli) were enumerated using quantitative plating. Total bacterial counts were performed using FISH. Bacterial DNA was analysed by quantitative Real-Time PCR for the detection of Atopobium spp., Bacteroides-Prevotella-Porphyromonas group, Clostridium coccoides-Eubacterium rectale group, Clostridium leptum subgroup, Clostridium difficile, Clostridium perfringens, Desulfovibrio desulfuricans group, and Bifidobacterium spp. Statistical analysis was performed using Mann-Whitney U tests or Paired Wilcoxon tests. P < 0.05 was considered statistically significant. Results: No significant differences in total bacterial counts were seen between the treatment and placebo group. Using quantitative plating, no significant differences were seen for the different bacterial groups between both groups. Significantly lower bacterial counts were seen in both groups for clostridia for S2 compared to S1 and S3. In the placebo group, significantly higher amounts of bifidobacteria and lactobacilli were detected for S2 compared to S1. Using qRT-PCR, significantly lower counts for D. desulfuricans group were seen in the placebo group compared to the treatment group. In the treatment group significant differences were seen only for S2 compared to S3, whereas in the placebo group, no significant differences were detected for the different bacterial groups at the different sampling points. Analysis at the IBS subtype level revealed differences in microbiota between the different subtypes. Conclusions: Although significant changes in the microbiota were seen during intake of LcS in the treatment group, differences in microbial populations between both groups were not significant. Interestingly, specific findings are likely to be associated with a specific IBS subtype rather than with IBS in general. Objective: To asses the chest radiography findings of patients with H1N1 Influenza virus infection. Review of radiological findings of 110 patients with confirmed influenza A virus infection admitted to two Hospitals in Cantabria, Spain. The radiological findings were characterized by pattern of opacities, lung distribution and, presence or absence of pleural effusions or hiliar or mediastinal adenopahies. The initial radiography was abnormal in 39 (34%) patients, but only 28 (25%) were related exclusively to the influenza A virus infection. Characteristic imaging findings in these 28 patients included: parenchymal consolidation 68%, consolidation plus ground glass 25% and ground glass opacities in 7%. Seventeen patients had a diffuse distribution of the opacities, in 61% it was bilateral, and lower and middle zones were those most frequently affected. No pleural effusions or pathological mediastinal lymph nodes were seen in this initial radiography. Patients with abnormal chest X-ray were more frequently men, smokers, had dyspnoea, pleuritic pain and diarrhoea. Conclusions: Although more of patients with influenza virus A infection showed normal chest X-ray, those with abnormal chest radiographies, characteristically showed areas of consolidation with or without ground glass affectation, bilateral and diffuse distribution, and predilection for lower and middle zones. Pleural effusion or mediastinal adenopahty were not findings seen in the initial radiography.
Title: R2250 H1N1 virus infection: review of chest radiographic findings
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Paragraph: Microglia and macrophages are related cell types that play an important role in the pathogenesis of MS and EAE. This chapters reviews the role of these cells in the normal brain and their contribution to inflammatory demyelinating disease, including their role in antigen presentation, co-stimulation, and production of cytokines and other inflammatory mediators The microglia, a brain-resident, non-neuronal cell and the blood-derived or haematogenous macrophage represent two related cell types involved in key events in the development of pathology in Multiple Sclerosis and its autoimmune animal model, the Experimental Allergic Encephalomyelitis. Microglia and macrophages fulfil a variety of different functions, but they are also recognized for their ability to act as a particularly fine sensor of brain pathology. Both cell types are rapidly activated and recruited to sites of infection, neurodegeneration, stroke, autoimmune inflammatory models such as EAE and its presumptive human counterpart, multiple sclerosis. Microglia and macrophages are stimulated by a variety of cytokines, neurotransmitters, modulators and putative neurotoxins, extracellular matrix molecules and proteases present in the inflamed central nervous system. Moreover, both cell types are plastic in their morphology and cellular identity. The presence of dying cells and cell debris will cause a transformation of phagocytic microglia into a detached, rounded and migratory or amoeba-like (amoeboid) macrophage (Streit et al., 1988; Bohatschek et al., 2001). This also works in reverse: surrounded by a CNS environment, non-phagocytic macrophages freshly recruited from the blood stream will gradually develop
Title: Chapter A7 ROLE OF MICROGLIA AND MACROPHAGES IN EAE
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