Instructions: Given a passage, construct a question on the basis of the information present in the passage. Construct the question in such a way that (i) it is unambiguous, (ii) it is answerable from the passage, (iii) its answer is unique (iv) it answer uses text phrases from the passage. Avoid creating questions that (i) can't be answered correctly without actually understanding the passage and (ii) uses the same words or phrases given in the passage.
Input: Knowledge of sepsis-related end-organ inflammation invivo is limited. We investigated the cytokine response in skin and in serum in sepsis and its relation to multiorgan failure (MOF) and survival.', 'Cytokines were analysed in serum and in suction blister fluid of intact skin of 44 patients with severe sepsis and 15 healthy controls. Blister fluid and serum samples were collected within 48h of the first sepsis-induced organ failure. This is a substudy of a larger follow-up study on wound healing in sepsis.', 'Cytokine levels were higher in patients with sepsis vs. controls (interleukin [IL]-10, blisters: 65.9 vs. 4.3pg/ml, P<0.001, serum: 25.7 vs. 4.5pg/ml, P=0.004; IL-6, blisters: 41.9 vs. 0.03pg/ml, P<0.001, serum: 45.5 vs. 2.1pg/ml, P<0.001). Patients with MOF had higher levels of IL-10 (116.4 vs. 21.3pg/ml, P=0.015), IL-4 (0.7 vs. 0.07pg/ml, P=0.013) and basic fibroblast growth factor (bFGF) (25.9 vs. 9.5pg/ml, P=0.027) in blister fluid than patients without MOF. In blister fluid, survivors had lower levels of IL-10 (43.3 vs. 181.9pg/ml, P=0.024) and bFGF (15.8 vs. 31.9pg/ml, P=0.006) than non-survivors. In serum, survivors had higher levels of vascular endothelial growth factor (VEGF) (152.2 vs. 14.7pg/ml, P=0.012) and lower levels of IL-6 (38.5 vs. 91.1pg/ml, P=0.011) than non-survivors. The blister fluid levels of bFGF, TNF and VEGF did not correlate with the serum levels.
Output:
Do blister fluid and serum cytokine levels in severe sepsis in humans reflect skin dysfunction?