In this task, you are given a multiple-choice question about healthcare. Answer the question based on your information and classify your answers into '1', '2', '3', and '4'.

Question: Sulfasalazine is used for the treatment of ulcerative colitis. It is a prodrug of 5-aminosalicylic acid that is activated by a process of:
Options: <1> Hydrolysis of an ester. <2> Hydrolysis of an amide. <3> Oxidative dealkylation. <4> Reducing rupture of the azoderivative. <5> Aromatic hydroxylation.
4

Question: The differential modification of RNA:
Options: <1> It can result in multiple products from one gene. <2> It occurs thanks to the transcription bubble. <3> It is catalyzed by RNA polymerase I. <4> It is catalyzed by RNA polymerase II. <5> It is catalyzed by RNA polymerase III.
1

Question: In the determination of water hardness due to the presence of Ca2 + and Mg2 + ions, the titrant reagent is used:
Options: <1> Methyl orange. <2> A standard solution of potassium chloride. <3> Black Eriochrome T. <4> A standard solution of ethylenediaminetetraacetic acid (EDTA). <5> A solution of AEDT previously standardized against a solution of potassium phthalate.
4