Given a scientific passage and an answer, generate a question for the given answer.

Ex Input:
Passage: Most plant cells have a large central vacuole . It can make up as much as 90 percent of a plant cell's total volume. The central vacuole is like a large storage container. It may store substances such as water, enzymes, and salts. It may have other roles as well. For example, the central vacuole helps stems and leaves hold their shape. It may also contain pigments that give flowers their colors. Answer: vacuole

Ex Output:
Most plant cells have a large central what?

Ex Input:
Passage: A genetic disorder that is caused by a mutation can be inherited. Therefore, people with a genetic disorder in their family may be concerned about having children with the disorder. Professionals known as genetic counselors can help them understand the risks of their children being affected. If they decide to have children, they may be advised to have prenatal ("before birth") testing to see if the fetus has any genetic abnormalities. One method of prenatal testing is amniocentesis. In this procedure, a few fetal cells are extracted from the fluid surrounding the fetus, and the fetal chromosomes are examined. Answer: children being affected

Ex Output:
Professionals known as genetic counselors can help them understand the risks of?

Ex Input:
Passage: Orbital Energies and Atomic Structure The energy of atomic orbitals increases as the principal quantum number, n, increases. In any atom with two or more electrons, the repulsion between the electrons makes energies of subshells with different values of l differ so that the energy of the orbitals increases within a shell in the order s < p < d < f. Figure 6.25 depicts how these two trends in increasing energy relate. The 1s orbital at the bottom of the diagram is the orbital with electrons of lowest energy. The energy increases as we move up to the 2s and then 2p, 3s, and 3p orbitals, showing that the increasing n value has more influence on energy than the increasing l value for small atoms. However, this pattern does not hold for larger atoms. The 3d orbital is higher in energy than the 4s orbital. Such overlaps continue to occur frequently as we move up the chart. Answer: it increases

Ex Output:
How is the energy of an atomic orbital affected as the principal quantum number increases?