QUES: A series of experiments performed from the late 1800s to the early 1900s revealed that diabetes is caused by the absence of a substance normally produced by the pancreas. In 1869, Oskar Minkowski and Joseph von Mering found that diabetes could be induced in dogs by surgical removal of the pancreas. In 1921, Canadian professor Frederick Banting and his student Charles Best repeated this study, and found that injections of pancreatic extract reversed the symptoms produced by pancreas removal. Soon, the extract was demonstrated to work in people, but development of insulin therapy as a routine medical procedure was delayed by difficulties in producing the material in sufficient quantity and with reproducible purity. The researchers sought assistance from industrial collaborators at Eli Lilly and Co. based on the company's experience with large scale purification of biological materials. Chemist George Walden of Eli Lilly and Company found that careful adjustment of the pH of the extract allowed a relatively pure grade of insulin to be produced. Under pressure from Toronto University and a potential patent challenge by academic scientists who had independently developed a similar purification method, an agreement was reached for non-exclusive production of insulin by multiple companies. Prior to the discovery and widespread availability of insulin therapy the life expectancy of diabetics was only a few months.

What is purification caused from?
What is the answer?
ANS: unanswerable
QUES: The gut is generally an almost straight tube supported by the mesenteries (vertical partitions within segments), and ends with the anus on the underside of the pygidium. However, in members of the tube-dwelling family Siboglinidae the gut is blocked by a swollen lining that houses symbiotic bacteria, which can make up 15% of the worms' total weight. The bacteria convert inorganic matter such as hydrogen sulfide and carbon dioxide from hydrothermal vents, or methane from seeps to organic matter that feeds themselves and their hosts, while the worms extend their palps into the gas flows to absorb the gases needed by the bacteria.

What foods come from seeps?
What is the answer?
ANS: unanswerable
QUES: Florida High Speed Rail was a proposed government backed high-speed rail system that would have connected Miami, Orlando, and Tampa. The first phase was planned to connect Orlando and Tampa and was offered federal funding, but it was turned down by Governor Rick Scott in 2011. The second phase of the line was envisioned to connect Miami. By 2014, a private project known as All Aboard Florida by a company of the historic Florida East Coast Railway began construction of a higher-speed rail line in South Florida that is planned to eventually terminate at Orlando International Airport.

From North Florida, where will All Aboard Florida stretch to?
What is the answer?
ANS:
unanswerable