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Given a passage, construct a question on the basis of the information present in the passage. Construct the question in such a way that (i) it is unambiguous, (ii) it is answerable from the passage, (iii) its answer is unique (iv) it answer uses text phrases from the passage. Avoid creating questions that (i) can't be answered correctly without actually understanding the passage and (ii) uses the same words or phrases given in the passage.

To evaluate the effect of Ac-hE-18A-NH2 on TNF- secretion and mRNA expression in ox-LDL-stimulated RAW264.7 macrophages and to elucidate the possible mechanisms.', "Macrophages were incubated in the medium containing various concentrations of Ac-hE18A-NH2 (1-50 g/mL) with ox-LDL (50 g/mL) stimulated. The TNF- level and intracellular cholesterol content were measured by commercially available quantitation kits following the manufacturer's instructions. TNF- and ATP-binding cassette transporter A1 (ABCA1) mRNA expression were detected by real-time PCR. ABCA1 and IB protein -expression in the macrophages were determined by Western blot. NF-B activity was evaluated by electrophoretic mobility shift assay (EMSA).", 'Ox-LDL stimulation induced a significant increase in TNF- secretion, mRNA expression, cholesterol accumulation and nuclear factor-B (NF-B) activity in RAW264.7 macrophages. Ac-hE-18A-NH2 reduced TNF- secretion and mRNA expression, up-regulated the ABCA1 mRNA and protein expression, reduced the intracellular cholesterol content, and inhibited NF- B activation in a dose-dependent manner. Under the same condition and the same concentration, Ac-hE-18A-NH2 was more efficient than D-4F (apoA-I mimetic peptide) in inhibiting the inflammatory response induced by ox-LDL in the macrophages.
Output:
Does [ Ac-hE-18A-NH2 inhibit the inflammatory response induced by ox-LDL via inhibiting NF-B activation in RAW264.7 macrophages ]?