In this task, you're given a paragraph and title from the research paper. Your task is to classify whether the given title is suitable or not for the research paper based on the given paragraph. Return "True" if title is proper according to paragraph else "False".

Input: Consider Input: Paragraph: Chitin is a linear polysaccharide of N-acetylglucosamine, which is highly abundant in nature and mainly produced by marine crustaceans. Chitosan is obtained by hydrolytic deacetylation. Both polysaccharides are renewable resources, simply and cost-effectively extracted from waste material of fish industry, mainly crab and shrimp shells. Research over the past five decades has revealed that chitosan, in particular, possesses unique and useful characteristics such as chemical versatility, polyelectrolyte properties, gel-and film-forming ability, high adsorption capacity, antimicrobial and antioxidative properties, low toxicity, and biocompatibility and biodegradability features. A plethora of chemical chitosan derivatives have been synthesized yielding improved materials with suggested or effective applications in water treatment, biosensor engineering, agriculture, food processing and storage, textile additives, cosmetics fabrication, and in veterinary and human medicine. The number of studies in this research field has exploded particularly during the last two decades. Here, we review recent advances in utilizing chitosan and chitosan derivatives in different technical, agricultural, and biomedical fields. A large number of chitosan-based composites were investigated for removal of metal ions from aqueous solutions. They include chitosan-polymer macromolecular complexes (as cellulose, cellulosic matrix like cotton fibers, alginate, polyvinyl alcohol, polyvinyl chloride), chitosan ceramics, as well as clay and silicate composites (bentonite, montmorillonite, perlite, and zeolite) (Wan Ngah et al. 2011) . Due to the vast number of scientific publications on chitosan-based adsorption that have been published, only a representative sample is depicted for Cr(VI) and Cu(II). Cognate composites were devised as adsorbents of other metal ions (Cd, As, Fe, Pb, Co, Pb, Hg, Ni, Zn, U) that can be found in the detailed reviews of Reddy and Lee (
Title: Chitin/Chitosan: Versatile Ecological, Industrial, and Biomedical Applications

Output: False

Input: Consider Input: Paragraph: Background: Bacterial pneumonia (BP) is an inflammation of the lower airways and lung parenchyma secondary to bacterial infection. The pathogenesis of BP in dogs is complex and the role of canine respiratory viruses has not been fully evaluated. Objectives: The aim of this study was to investigate the occurrence of viral co-infections in dogs with BP and to assess demographic or clinical variables as well as disease severity associated with viral co-infections. Animals: Twenty household dogs with BP caused by opportunistic bacteria and 13 dogs with chronic (>30 days) tracheobronchitis caused by Bordetella bronchiseptica (BBTB). Methods: Prospective cross-sectional observational study. Diagnosis was confirmed by clinical and laboratory findings, diagnostic imaging, and cytologic and microbiologic analysis of bronchoalveolar lavage or transtracheal wash fluid. Canine parainfluenza virus (CPIV), canine adenovirus, canine herpes virus, canine influenzavirus, canine distemper virus, canine respiratory coronavirus (CRCoV) and canine pneumovirus, as well as B. bronchiseptica and Mycoplasma spp. were analyzed in respiratory samples using PCR assays. Results: CPIV was detected in 7/20 and CRCoV in 1/20 dogs with BP. Respiratory viruses were not detected in dogs with BBTB. There were no significant differences in clinical variables between BP dogs with and without a viral co-infection. Conclusion and Clinical Importance: Respiratory viruses were found frequently in dogs with BP and may therefore play an important role in the etiology and pathogenesis of BP. Clinical variables and disease severity did not differ between BP dogs with and without viral co-infection.
Title: Co-infections with Respiratory Viruses in Dogs with Bacterial Pneumonia

Output: True

Input: Consider Input: Paragraph: In order to differentiate recent isolates of avian infectious bronchitis virus (IBV) in Taiwan, polymerase chain reaction (PCR), restriction fragment length polymorphism (RFLP), and direct sequencing methods were used to type 25 IBV Taiwan isolates. Two conserved sequences that flank the hypervariable region I (HVR I) in the N-terminus of S1 protein gene were chosen as primers. Sequences of 228-231 base pairs (bp) were amplified by PCR from 25 Taiwan isolates and 4 reference strains (H120, Conn, JMK, Holte). PCR products were digested with 5 restriction endonucleases, BsoFI, DdeI, MboII, AluI, RsaI, and different IBV isolates were grouped according to their RFLP patterns. The RFLP patterns of the 4 reference strains in this study matched the published sequences in GenBank. Except 1 vaccine strain, the other 24 Taiwan isolates were different from these 4 and 18 other IBV strains whose sequences were published. The data from PCR-RFLP and sequencing of IBV genomes showed that the 24 Taiwan isolates can be divided into 2 distinct groups, I and II. Seven RFLP patterns are identified in group I and only 1 in group II. 1678 C.-H. Wang and C.-T. Tsai antibodies that normally form the basis for comparison of IBV isolates are induced largely by the N-terminus of the S1 protein [7, 9, 11-]. In the present study, we used reverse transcription polymerase chain reaction (RT-PCR) coupled with restriction fragment length p o l y m o r p h i s m (RFLP) of the R T -P C R products to classify our isolates. Using these techniques, we present a convenient m e t h o d for identifying the R F L P patterns of IBV isolates in Taiwan which are different from those of k n o w n IBV strains. Materials and methods Twenty-five Taiwan isolates of IBV were used in this study. and V1397. Four sets of primers successfully used by other [1, 12, 13, 18] and one new set of primers were used in this study. The published primers included S1 [12], $2 [13], M-N [-1], and N primers [18] . For the new primers, the S1 sequence of the 22 published IBV were analyzed using the Clustal method to find conserved sequences. The new primers (C2U-C3L) were located in the N-terminus of the S1 gene which contains hypervariabte region I (HRV I, nucleotides, nt 142 320); this region is reponsible for induction of antibodies to the IBV that have been the basis for defining of different IBV serotypes [9, 11] . The sequences and locations of the new primers used for the PCR were C2U: TGGTT GGCAT TTACA CGGGG (114-133) and C3L: CAATG GGTAA CAAAC AC (341-325, as in Beaudette strain). Viral RNA was extracted as described [t2] with some modification. Briefly, guanidine thiocyanate (Sigma), and NaOAC were added to allantoic fluid, and extracted with phenolchloroform-isoamylalcohol. The RNA was further precipitated in isopropanol (Merck) and resuspended in alcohol and stored at -20 C until it was used in RT-PCR. Genetic typing of infectious bronchitis virus in Taiwan 1679 All glassware and double distilled water (DDW) were treated with 0.1% diethyl-pyrocarbonate (Sigma) for removing RNase contamination. The RT-PCR was accomplished in one step. The mixture included 10 ~tl of pfu polymerase 10 x buffer (Stratagene, USA), 8 gl of 2.5 mM dNTP (Stragene), 0.4 gl of 40 U/gl RNasin (Promega), 0.3 gl RTase (AMV, Promega), 1 ~tl primers (20 ~tM), 0.8 gl of 2.5 U/gl pfu polymerase (Stragene), 60 gl DDW and 20 gl viral RNA. The RT-PCR was conducted in a FTS-960 thermal cycler (Corbett Research, Australia) where the RT was performed at 42 C for 1 h. PCR was then performed during 35 cycles of denaturation at 94 C for 30 sec, annealing at 45 C for 1 rain, and polymerization at 72 C for 1 rain. The final polymerization step was conducted at 72 C for 7 rain. The PCR products were analyzed on a 2% gel containing ethidium bromide (0.5 gg/ml), and the amplified DNA was detected using an ultaviolet transilluminator.
Title: Genetic grouping for the isolates of avian infectious bronchitis virus in Taiwan
Output: False