Generate the answer for a given question. The question requires common knowledge of English. Make sure that the answer is in detail, long, consistent with the context, and requires general knowledge of English. To be less repetitive, please vary your language for each question. Give a proper explanation to answer, and if necessary, provide the definition of various terms. And conclude your answer if it's long. If you have used other resources (e.g., Merriam Webster's.) to answer the question, include citations in your answer. You don't need to paraphrase the context of your resources, but make sure that your answer is consistent and complete.
--------
Question: Consider the following two sentences: Statement 1: The parameters were calculated a priori, and the other variables were calculated iteratively. Statement 2: The parameters were calculated a priori, and the other variables were calculated iteratively. This may seem trivial, but the only difference between the two sentences is the way in which the , has been formatted. In the first statement the , is in italics. In the second, the , is not in italics. Does it depend on the writer whether or not to italicize the punctuation marks adjoining the affected words? Or is there a definite rule governing this? The same question can be extended to other types of formatting, e.g. bold.

Answer: Here's one style manual's answer to that question: Italics do not include punctuation marks (end marks or parentheses, for instance) next to the words being italicized unless those punctuation marks are meant to be considered as part of what is being italicized: 'Have you read Stephen King's Pet Semetary?' (The question mark is not italicized here.) Also, do not italicize the apostrophe-s which creates the possessive of a title: 'What is the Courant's position on this issue?' This is really a style-manual problem, not an English usage or grammar problem. Each publication has its house style. Newspapers have their own style manuals, academic fields have theirs, and technical fields have theirs. There is no definite rule.

Question: What's an antonym for 'virgin'? A single word would be preferable.

Answer: This is OP's second question on EL&U, and the previous one refers to the fact that he's 'writing an application that deals with blind studies'. In light of that, I assume OP is effectively looking for a medical term. I'm not a doctor, but these people are, and they use the term coitally experienced. Here's an NGram showing they're not alone. In case anyone objects that (besides not being single-word) coitally experienced applies to males as well as females, I suggest post-coitarchal or perhaps my own neologism coitarchated (which I think would be well enough understood on first encounter by people working in that area). Coitarche (first experience of full intercourse) didn't really enter the lexicon until the early '80s, but it's clearly formed by association with menarche (a pubescent girl's first period), so I think that's good enough to say coitarche and derivatives relate more to the female than the male side of the experience.

Question: When should whilst be used instead of while? For example, should I use the first or the second sentence? They don't do this whilst they do that. They don't do this while they do that. Which would be correct?.

Answer:
I believe while and whilst are interchangeable, but whilst is more archaic and adds a nice flavoring to the sentence. That being said, 'whilst' doesn't seem to come up so often in the middle of a sentence as you had it there, so I would use 'while' in that case, even though both are grammatically correct. Whilst seems more apt to be put at the beginnings of sentences, e.g. Whilst going about my merry way I chanced upon a vagabond. Taking this serendipitous opportunity to satiate my bloodlust I decapitated him with a fork.