Given an abstract, generate a keyword (a noun phrase) that best describes the focus or contribution of the paper. Such keywords can be directly from the given abstract or outside it.

Q: Abstract: A 52K glycoprotein is secreted by human breast cancer cells in culture after estrogen stimulation. Using monoclonal antibodies, we have quantitated and characterized the corresponding proteins of the cell compartment. Using pulse-chase experiments, we have shown that about 40% of the 52K protein is secreted, the majority being successively processed into a 48K and a 34K protein. This last protein is very stable. The processing is inhibited by lysosomotropic agents and leupeptin, suggesting that it occurs in acidic vesicles, such as lysosomes or endosomes. Estradiol increased the intracellular level of immunoreactive 52K related proteins by 4-fold. Its effect is, however, more obvious in the medium, since there is a constitutive level in the cell. The stimulatory effects of estradiol on [3H]mannose and [35S]methionine incorporation into these proteins were similar and the endoglycosydase H sensitivity of the proteins was not altered, suggesting that estradiol did not modulate the glycosylation step. Antiestrogens did not stimulate synthesis and glycosylation of the 52K related proteins. Estradiol also increased the stability of the 52K precursor as well as that of total proteins. We conclude that the secreted 52K protein is the precursor of two cellular proteins of 48K and 34K. Estradiol stimulates both the intracellular accumulation of these proteins and the secretion of the precursor.

A: Ammonium Chloride
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Q: Abstract: We reviewed 72 patients with penetrating trauma to the extremities who underwent arteriography for proximity injury only. None of the patients had clinical evidence of vascular trauma. There were 62 males and ten females, with a mean age of 29.9 years. Gunshot wounds were the most common cause of injury (91.7%) and the thigh was the most common site of injury (47.2%). A normal arteriogram was found in 55 of 72 patients (76.4%). The remaining 17 patients (23.6%) had arteriographic abnormalities that did not warrant surgery. Only one patient was explored (1.4%) for spasm of the popliteal artery. No vascular injury was found at surgery. This study suggests that routine arteriography in proximity injury only may be unnecessary and that these patients could safely be admitted to the hospital for a 24-hour period of observation.

A: Adult
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Q: Abstract: In the radial hemolysis test for rubella virus-specific antibodies, sera obtained shortly after primary rubella produce a characteristically altered hemolysis zone, termed soft hemolysis. The occurrence of soft hemolysis was analyzed statistically in 6,723 sera. The specificity of soft hemolysis for infection with rubella virus was 99.0%. Its sensitivity in primary infections was 97.3%, and the predictive value for rubella serodiagnosis (increase in IgG level or detectable IgM) was 91.2%. The temporal association of soft hemolysis with rubella was determined with sera obtained from 129 persons during late convalescence (70-310 days after onset of symptoms). Soft hemolysis disappeared by 190 days after the onset of symptoms and the presence of ordinary hemolysis ruled out primary rubella in the preceding 70-100 days. Soft hemolysis was caused by IgG antibody to rubella virus, and thus represents a new serodiagnostic principle. This technique, together with an Igm assay, allows rapid diagnosis of recent rubella from a single serum sample.

A:
Antibodies, Viral
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