instruction:
In this task, you are given a question and a context passage. You have to answer the question based on the given passage.
question:
What needs to decrease before pituitary growth hormone can increase in the blood?, Context: In addition to the negative consequences of sleep deprivation, sleep and the intertwined circadian system have been shown to have strong regulatory effects on immunological functions affecting both the innate and the adaptive immunity. First, during the early slow-wave-sleep stage, a sudden drop in blood levels of cortisol, epinephrine, and norepinephrine induce increased blood levels of the hormones leptin, pituitary growth hormone, and prolactin. These signals induce a pro-inflammatory state through the production of the pro-inflammatory cytokines interleukin-1, interleukin-12, TNF-alpha and IFN-gamma. These cytokines then stimulate immune functions such as immune cells activation, proliferation, and differentiation. It is during this time that undifferentiated, or less differentiated, like naive and central memory T cells, peak (i.e. during a time of a slowly evolving adaptive immune response). In addition to these effects, the milieu of hormones produced at this time (leptin, pituitary growth hormone, and prolactin) support the interactions between APCs and T-cells, a shift of the Th1/Th2 cytokine balance towards one that supports Th1, an increase in overall Th cell proliferation, and naive T cell migration to lymph nodes. This milieu is also thought to support the formation of long-lasting immune memory through the initiation of Th1 immune responses.
answer:
cortisol, epinephrine, and norepinephrine

question:
Who were the Spanish monarchs that financed Christopher Columbus?, Context: The 1469 marriage of Isabella I of Castile and Ferdinand II of Aragon and the 1479 death of John II of Aragon led to the creation of modern-day Spain. In 1492, Granada was captured from the Moors, thereby completing the Reconquista. Portugal had during the 15th century particularly under Henry the Navigator gradually explored the coast of Africa, and in 1498, Vasco da Gama found the sea route to India. The Spanish monarchs met the Portuguese challenge by financing the expedition of Christopher Columbus to find a western sea route to India, leading to the discovery of the Americas in 1492.
answer:
Isabella I of Castile and Ferdinand II of Aragon

question:
What are the elements so necessary?, Context: Interspersed repeats mainly come from transposable elements (TEs), but they also include some protein coding gene families and pseudogenes. Transposable elements are able to integrate into the genome at another site within the cell. It is believed that TEs are an important driving force on genome evolution of higher eukaryotes. TEs can be classified into two categories, Class 1 (retrotransposons) and Class 2 (DNA transposons).
answer:
TEs are an important driving force on genome evolution of higher eukaryotes