The central oscillator generates a self-sustaining rhythm and is driven by two interacting feedback loops that are active at different times of day. The morning loop consists of CCA1 (Circadian and Clock-Associated 1) and LHY (Late Elongated Hypocotyl), which encode closely related MYB transcription factors that regulate circadian rhythms in Arabidopsis, as well as PRR 7 and 9 (Pseudo-Response Regulators.) The evening loop consists of GI (Gigantea) and ELF4, both involved in regulation of flowering time genes. When CCA1 and LHY are overexpressed (under constant light or dark conditions), plants become arrhythmic, and mRNA signals reduce, contributing to a negative feedback loop. Gene expression of CCA1 and LHY oscillates and peaks in the early morning, whereas TOC1 gene expression oscillates and peaks in the early evening. While it was previously hypothesised that these three genes model a negative feedback loop in which over-expressed CCA1 and LHY repress TOC1 and over-expressed TOC1 is a positive regulator of CCA1 and LHY, it was shown in 2012 by Andrew Millar and others that TOC1 in fact serves as a repressor not only of CCA1, LHY, and PRR7 and 9 in the morning loop but also of GI and ELF4 in the evening loop. This finding and further computational modeling of TOC1 gene functions and interactions suggest a reframing of the plant circadian clock as a triple negative-component repressilator model rather than the positive/negative-element feedback loop characterizing the clock in mammals.
What was the previous belief of feedback in the gene loop?
negative feedback loop

Input: Light-emitting diode
In the US, one kilowatt-hour (3.6 MJ) of electricity currently causes an average 1.34 pounds (610 g) of CO
2 emission. Assuming the average light bulb is on for 10 hours a day, a 40-watt bulb will cause 196 pounds (89 kg) of CO
2 emission per year. The 6-watt LED equivalent will only cause 30 pounds (14 kg) of CO
2 over the same time span. A building's carbon footprint from lighting can therefore be reduced by 85% by exchanging all incandescent bulbs for new LEDs if a building previously used only incandescent bulbs.

How much can a building's carbon footprint be reduced by switching to LEDs?
Output: 85%

Input: Article: In 1979, the Japanese state broadcaster NHK first developed consumer high-definition television with a 5:3 display aspect ratio. The system, known as Hi-Vision or MUSE after its Multiple sub-Nyquist sampling encoding for encoding the signal, required about twice the bandwidth of the existing NTSC system but provided about four times the resolution (1080i/1125 lines). Satellite test broadcasts started in 1989, with regular testing starting in 1991 and regular broadcasting of BS-9ch commencing on November 25, 1994, which featured commercial and NHK programming.

Now answer this question: When did regular broadcasting of BS-9ch start?

Output: November 25, 1994

Article: The SpanishAmerican War was a short decisive war marked by quick, overwhelming American victories at sea and on land against Spain. The Navy was well-prepared and won laurels, even as politicians tried (and failed) to have it redeployed to defend East Coast cities against potential threats from the feeble Spanish fleet. The Army performed well in combat in Cuba. However, it was too oriented to small posts in the West and not as well-prepared for an overseas conflict. It relied on volunteers and state militia units, which faced logistical, training and food problems in the staging areas in Florida. The United States freed Cuba (after an occupation by the U.S. Army). By the peace treaty Spain ceded to the United States its colonies of Puerto Rico, Guam, and the Philippines. The Navy set up coaling stations there and in Hawaii (which voluntarily joined the U.S. in 1898). The U.S. Navy now had a major forward presence across the Pacific and (with the lease of Guantanamo Bay Naval Base in Cuba) a major base in the Caribbean guarding the approaches to the Gulf Coast and the Panama Canal.

Question: Where did the American army fight the Spanish in the Spanish American War?
Ans: Cuba

Here is a question about this article: Schwarzenegger admitted that he has "behaved badly sometimes" and apologized, but also stated that "a lot of [what] you see in the stories is not true". This came after an interview in adult magazine Oui from 1977 surfaced, in which Schwarzenegger discussed attending sexual orgies and using substances such as marijuana. Schwarzenegger is shown smoking a marijuana joint after winning Mr. Olympia in the 1975 documentary film Pumping Iron. In an interview with GQ magazine in October 2007, Schwarzenegger said, "[Marijuana] is not a drug. It's a leaf. My drug was pumping iron, trust me." His spokesperson later said the comment was meant to be a joke.
What is the answer to this question: What drug does the documentary Pumping Iron show Schwarzenegger using?
****
So... marijuana

Article: Due to its location, the Punjab region came under constant attacks and influence from the west and witnessed centuries of foreign invasions by the Greeks, Kushans, Scythians, Turks, and Afghans. The city of Taxila, founded by son of Taksh the son Bharat who was the brother of Ram. It was reputed to house the oldest university in the world,[citation needed] Takshashila University. One of the teachers was the great Vedic thinker and politician Chanakya. Taxila was a great centre of learning and intellectual discussion during the Maurya Empire. It is a UN World Heritage site, valued for its archaeological and religious history.

Question: Who has invaded Punjab?
Ans:
Greeks, Kushans, Scythians, Turks, and Afghans