Given a passage and a query based on the passage, generate an unambiguous, concise and simple answer to the query from information in the passage. Note that the answer may not be present in exact form.

Input: Consider Input: The 2004-2005 crop year yielded 13.4 million U.S. tons (12.2 million metric tons) of stalks and 1.56 million U.S. tons (1.42 million metric tons) of raw sugar. Putting Florida's cane crop into perspective, prior to the Cuban embargo in 1961 Florida had only 50,000 acres (20,234 hectares) of sugarcane. What is the value of Florida's sugar crop? Recoverable sugar, by weight, has increased from below 10 percent in 1984 to 11.6 percent in 2000-01. In 2004-05, Florida contributed an estimated 51.3% of the cane sugar and 21.4% of the total (from sugarcane and beets combined) sugar produced in the United States. Where does the rest of the sugar produced in the U.S. come from? In the sugar-production process, raw sugar is moved from warehouse to refinery, where the sugar is re-dissolved and refined to obtain the purity required for white sugar crystals. Credits: Sugar Cane Growers Cooperative of Florida In 2007-08, Florida contributed an estimated 48 percent of the cane sugar and 24.3 percent of the total (from sugarcane and beets, combined) sugar produced in the United States. In comparison, Florida's 2007-2008 sugarcane crop year yielded 13.3 million U.S. tons (12.1 million metric tons) of stalks and 1.55 million U.S. tons (1.41 million metric tons) of raw sugar. Placing the current size of Florida's sugarcane crop into perspective historically, prior to the Cuban embargo in 1961, Florida had only 50,000 acres (20,234 hectares) of sugarcane. All Florida sugarcane is destined for one of the five sugarcane mills in south Florida. Corporations that grow their own cane and extract the sugar in their own mills produce over 65% of the cane. Independent growers for grower-owned cooperative mills produce another large portion, 25-30%. In 2004, Florida's raw sugar crop was valued at roughly $550 million (http://www.ers.usda.gov/StateFacts/FL.htm ). Most of the fibrous portion of the cane stalks (bagasse) was burned as fuel for the mills and saved an estimated 113 million gallons of fuel oil or 2.1 billion kilowatt hours of electricity. How much sugarcane is grown in Florida? In recent years there has been a slight decline in commercial sugarcane production in Florida. Sugarcane acreage has decreased from a high of 454,400 acres (183,727 hectares) in the 2000-2001crop year to approximately 400,000 acres (161,874 hectares) for the 2004-2005 crop year. The number of farms growing sugar beets declined slightly from 4,022 to 3,913, while average area harvested per farm also rose slightly from 312 to 319 acres. The number of sugarcane farms dropped from 692 to 666, but have fallen from 953 sugarcane farms in the United States in 2002. Sugarcane production. Sugarcane is a crop that can be grown throughout Florida. In many areas of the state, sugarcane is grown only as a hobby crop for syrup production or as a source of chewing cane. However, in South Florida, near Lake Okeechobee, sugarcane is grown commercially for the production of crystal or white sugar. An average stalk, therefore, has about 2.6 pounds (1.2 kilograms) of juice, which is roughly 11 percent sugar by weight. Thus, an average stalk contains about 0.3 pounds (0.14 kilograms) of sugar. Cane sugar and beet sugar are identical in chemistry and quality. Query: how many acres of sugar cane in florida

Output: In 1961 Florida had only 50,000 acres of sugarcane.

Input: Consider Input: Proteins are separated by charge in agarose because the pores of the gel are too large to sieve proteins. Gel electrophoresis can also be used for separation of nanoparticles. Gel electrophoresis uses a gel as an anticonvective medium and/or sieving medium during electrophoresis, the movement of a charged particle in an electrical field. What are the components of a gel electrophoresis chamber? What kinds of macromolecules can you look at with gel electrophoresis? Do you need to use different techniques for the different kinds of macromolecules? How do you visualize the macromolecules in the gel? What are real-life examples of what gel electrophoresis is used for? Bibliography Once these are separated, a photo is taken of the gel, providing a picture of the separated molecular parts. This process is called electrophoresis and helps us to better understand and identify the molecular components of living things. Forensics In the world of forensics, gel electrophoresis is used to obtain a DNA fingerprint of a criminal. This means that scientists can accurately tell whether two pieces of DNA, one found at a crime scene and one belonging to a suspect, are matches. Gel electrophoresis is a method for separation and analysis of macromolecules and their fragments, based on their size and charge. It is used in clinical chemistry to separate proteins by charge and/or size and in biochemistry and molecular biology to separate a mixed population of DNA and RNA fragments by length, to estimate the size of DNA and RNA fragments or to separate proteins by charge. Nucleic acid molecules are separated by applying an electric field to move the negatively charged molec Gel Electrophoresis and SDS-PAGE Gel Electrophoresis which includes Protein Gel Electrophoresis Current Uses of Gel Electrophoresis -Gel electrophoresis is a technique used for the separation of deoxyribonucleic acid (DNA), ribonucleic acid (RNA), or protein molecules by using an electric field and a gel matrix. DNA Gel electrophoresis is usually used in order for investigations. DNA profiling is the process of determining an individual's DNA characteristics, called a DNA profile, that is very likely to be different in unrelated individuals, thereby being as unique to individuals as are fingerprints. DNA profiling with the aim of identifying not an individual but a species is called DNA barcoding. DNA profiling is most commonly used as a forensic technique in criminal investigations to identify an unidentified person or whose identity needs to be confirmed, or to place a person at a crime scene or to eliminate a person from consideration. Electrophoresis is used in DNA for fingerprints for forensics, paternity testing, checking PCR reactions, disease detection and to look for evolutionary relationships among organisms. A solution of negatively charged DNA molecules are pulled through gel using an electric field. Thanks to TV shows like CSI, many people are familiar with the use of gel to separate macromolecules like DNA. However, gel electrophoresis91112 can also be used to separate out proteins. Different proteins have different sizes, mainly due to the number of building blocks in their structure. Gel Electrophoresis is used to separate DNA fragments by their sizes and charges. It makes it possible to identify the differences and similarities between different plants and animals. Although there are some different types of Gel Electrophoresis such as Capillary and Agarose, this is how the general technology works: Quick Answer. Electrophoresis, also called gel electrophoresis, is used to separate the macromolecules of DNA, RNA and proteins. DNA and RNA are separated according to their size and length, while proteins are separated according to their charges. Query: how is gel electrophoresis used in the field of forensics

Output: In the world of forensics, gel electrophoresis is used to obtain a DNA fingerprint of a criminal.

Input: Consider Input: The Ultimate Guide to TSA Pre-Check. Updated 4 months ago. Book Your Lounge Now. In response to the attacks on September 11, 2001, the US government created the Transportation Security Administration (TSA) to develop policies to help secure nationwide transportation systems, including airports, highways, railroads, buses, and mass transit systems. If you do get approved for TSA Pre-Check, you can choose to receive your Known Traveler Number (KTN) either through the mail or online. When booking flights from airports that support Pre-Check, be sure to do so with an airline that TSA Pre-Check covers, and be sure to enter in your KTN. Your TSA Pre-Check eligibility is valid for 5 years, after which you will have to re-apply. Implementing a key component of the TSA's intelligence-driven, risk-based approach to security, TSA PreCheck enhances aviation security by placing more focus on pre-screening individuals who volunteer to participate to expedite the travel experience. No, not all airports have a TSA PreCheck lane, and those that do might not have TSA PreCheck at every checkpoint. Learn more about participating Southwest Airlines airports or for more information on checkpoint locations and hours of operation, visit tsa.gov. After verifying whether or not your departure airport has an open TSA PreCheck lane at your security checkpoint, look for signs with the TSA PreCheck logo at the entrance to the checkpoint lanes. While it was initially provided at only 4 airports, the TSA Pre-Check program is now available in over 160 US airports. 1 While it was formerly available only for domestic flights within the United States, TSA Pre-Check has expanded its reach to include certain international flights departing from US airports. Eligibility for TSA Pre-Check. If you are a US citizen and active elite member of any of the partner airlines' Frequent Flyer Programs, you may be invited to opt-in for TSA Pre-Check. If you choose to do so, you will only be eligible to go through TSA Pre-Check when you are flying on that FFP's airline. In order to be eligible for TSA Pre-Check for all airlines that allow for the service, you must have a Known Traveler Number (KTN). Not sure where you're posting from but there's far fewer interview sites for Global Entry than for TSA Pre-check. Do a little looking around on the Global Entry site to see where your closest interview will take place. Took about 8 business days to get the letter with my Pre-check number. I looked up the TSA precheck office that was nearest me, made an appointment through the TSA site. (Find locations here) Since approximately 2000 people apply to TSA Pre-Check on a daily basis, it may be advisable to make an appointment to avoid long waiting times. In order to determine your eligibility for Pre-Check, the TSA uses your fingerprints to check against law enforcement, immigration, and intelligence databases. TSA pre check must be renewed every 5 years. I have a credit card that pays for TSA precheck, so my cost was zero. But I would have paid because it is a game changer. While domestically I have been automatically put in TSA pre-check when traveling solo, and that was nice, I don't see the advantage for everyone. Query: how long does it take to get your ktn number after it is showing eligibility determined
Output: It takes 5 years to get your KTN number after it is showing eligibility determined.