You will be given a definition of a task first, then some input of the task.
Given a passage classify if the passage has a definite objective/aim/goal or not. Output '1' if the passage has a defininte objective/aim/goal and output '0' if the passage does not have a definite objective/aim/goal.

Pluripotent embryonic stem cells are considered to be an unlimited cell source for tissue regeneration and cell-based therapy. Investigating the molecular mechanism underlying the regulation of embryonic stem cell expansion is thus important. 14-3-3 proteins are implicated in controlling cell division, signaling transduction and survival by interacting with various regulatory proteins. However, the function of 14-3-3 in embryonic stem cell proliferation remains unclear.', 'In this study, we show that all seven 14-3-3 isoforms were detected in mouse embryonic stem cells. Retinoid acid suppressed selectively the expression of 14-3-3 isoform. Knockdown of 14-3-3 with siRNA reduced embryonic stem cell proliferation, while only 14-3-3 transfection increased cell growth and partially rescued retinoid acid-induced growth arrest. Since the growth-enhancing action of 14-3-3 was abrogated by -catenin knockdown, we investigated the influence of 14-3-3 overexpression on -catenin/GSK-3. 14-3-3 bound GSK-3 and increased GSK-3 phosphorylation in a PI-3K/Akt-dependent manner. It disrupted -catenin binding by the multiprotein destruction complex. 14-3-3 overexpression attenuated -catenin phosphorylation and rescued the decline of -catenin induced by retinoid acid. Furthermore, 14-3-3 enhanced Wnt3a-induced -catenin level and GSK-3 phosphorylation. DKK, an inhibitor of Wnt signaling, abolished Wnt3a-induced effect but did not interfere GSK-3/14-3-3 binding.
Output:
0