QUES: In the absence of suitable plate culture techniques, some microbes require culture within live animals. Bacteria such as Mycobacterium leprae and Treponema pallidum can be grown in animals, although serological and microscopic techniques make the use of live animals unnecessary. Viruses are also usually identified using alternatives to growth in culture or animals. Some viruses may be grown in embryonated eggs. Another useful identification method is Xenodiagnosis, or the use of a vector to support the growth of an infectious agent. Chagas disease is the most significant example, because it is difficult to directly demonstrate the presence of the causative agent, Trypanosoma cruzi in a patient, which therefore makes it difficult to definitively make a diagnosis. In this case, xenodiagnosis involves the use of the vector of the Chagas agent T. cruzi, an uninfected triatomine bug, which takes a blood meal from a person suspected of having been infected. The bug is later inspected for growth of T. cruzi within its gut.
What can Mycobacterium leprae and Treponema pallidum never be grown in?

ANS: unanswerable

QUES: Internationally, Victoria took a keen interest in the improvement of relations between France and Britain. She made and hosted several visits between the British royal family and the House of Orleans, who were related by marriage through the Coburgs. In 1843 and 1845, she and Albert stayed with King Louis Philippe I at chateau d'Eu in Normandy; she was the first British or English monarch to visit a French one since the meeting of Henry VIII of England and Francis I of France on the Field of the Cloth of Gold in 1520. When Louis Philippe made a reciprocal trip in 1844, he became the first French king to visit a British sovereign. Louis Philippe was deposed in the revolutions of 1848, and fled to exile in England. At the height of a revolutionary scare in the United Kingdom in April 1848, Victoria and her family left London for the greater safety of Osborne House, a private estate on the Isle of Wight that they had purchased in 1845 and redeveloped. Demonstrations by Chartists and Irish nationalists failed to attract widespread support, and the scare died down without any major disturbances. Victoria's first visit to Ireland in 1849 was a public relations success, but it had no lasting impact or effect on the growth of Irish nationalism.
How was the House of Orleans and the British Royal family unrelated?

ANS: unanswerable

QUES: The first written account of a game played was on October 15, 1862, on the Montreal Cricket Grounds. It was between the First Battalion Grenadier Guards and the Second Battalion Scots Fusilier Guards resulting in a win by the Grenadier Guards 3 goals, 2 rouges to nothing.[citation needed] In 1864, at Trinity College, Toronto, F. Barlow Cumberland, Frederick A. Bethune, and Christopher Gwynn, one of the founders of Milton, Massachusetts, devised rules based on rugby football. The game gradually gained a following, with the Hamilton Football Club formed on November 3, 1869, (the oldest football club in Canada). Montreal formed a team April 8, 1872, Toronto was formed on October 4, 1873, and the Ottawa FBC on September 20, 1876.
What was the first recorded set of devised rules of a Canadian football game?

ANS: unanswerable

QUES: At the centre of this wing is the famous balcony with the Centre Room behind its glass doors. This is a Chinese-style saloon enhanced by Queen Mary, who, working with the designer Sir Charles Allom, created a more "binding" Chinese theme in the late 1920s, although the lacquer doors were brought from Brighton in 1873. Running the length of the piano nobile of the east wing is the great gallery, modestly known as the Principal Corridor, which runs the length of the eastern side of the quadrangle. It has mirrored doors, and mirrored cross walls reflecting porcelain pagodas and other oriental furniture from Brighton. The Chinese Luncheon Room and Yellow Drawing Room are situated at each end of this gallery, with the Centre Room obviously placed in the centre.
What is the secondary theme of the Centre Room?

ANS:
unanswerable