Detailed Instructions: In this task, you're given a paragraph and title from the research paper. Your task is to classify whether the given title is suitable or not for the research paper based on the given paragraph. Return "True" if title is proper according to paragraph else "False".
Q: Paragraph: It has been demonstrated that, in addition to genomic RNA, sgmRNA is able to serve as a template for the synthesis of the negative-strand [()-strand] complement. However, the cis-acting elements on the positive-strand [(+)-strand] sgmRNA required for ()-strand sgmRNA synthesis have not yet been systematically identified. In this study, we employed real-time quantitative reverse transcription polymerase chain reaction to analyze the cis-acting elements on bovine coronavirus (BCoV) sgmRNA 7 required for the synthesis of its ()-strand counterpart by deletion mutagenesis. The major findings are as follows. (1) Deletion of the 5'-terminal leader sequence on sgmRNA 7 decreased the synthesis of the ()-strand sgmRNA complement. (2) Deletions of the 3' untranslated region (UTR) bulged stem-loop showed no effect on ()-strand sgmRNA synthesis; however, deletion of the 3' UTR pseudoknot decreased the yield of ()-strand sgmRNA. (3) Nucleotides positioned from 15 to 34 of the sgmRNA 7 3'-terminal region are required for efficient ()-strand sgmRNA synthesis. (4) Nucleotide species at the 3'-most position (1) of sgmRNA 7 is correlated to the efficiency of ()-strand sgmRNA synthesis. These results together suggest, in principle, that the 5'-and 3'-terminal sequences on sgmRNA 7 harbor cis-acting elements are critical for efficient ()-strand sgmRNA synthesis in BCoV.
Title: Identification of Cis-Acting Elements on Positive-Strand Subgenomic mRNA Required for the Synthesis of Negative-Strand Counterpart in Bovine Coronavirus
A:
False