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Finally, an introduced species may unintentionally injure a species that depends on the species it replaces. In Belgium, Prunus spinosa from Eastern Europe leafs much sooner than its West European counterparts, disrupting the feeding habits of the Thecla betulae butterfly (which feeds on the leaves). Introducing new species often leaves endemic and other local species unable to compete with the exotic species and unable to survive. The exotic organisms may be predators, parasites, or may simply outcompete indigenous species for nutrients, water and light.

Where does the Prunus spinosa leaf much sooner?
Answer: In Belgium
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An important guiding principle of Buddhist practice is the Middle Way (or Middle Path), which is said to have been discovered by Gautama Buddha prior to his enlightenment. The Middle Way has several definitions:

Guatama discovered the middle path before his what?
Answer: enlightenment
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The Gram stain, developed in 1884 by Hans Christian Gram, characterises bacteria based on the structural characteristics of their cell walls. The thick layers of peptidoglycan in the "Gram-positive" cell wall stain purple, while the thin "Gram-negative" cell wall appears pink. By combining morphology and Gram-staining, most bacteria can be classified as belonging to one of four groups (Gram-positive cocci, Gram-positive bacilli, Gram-negative cocci and Gram-negative bacilli). Some organisms are best identified by stains other than the Gram stain, particularly mycobacteria or Nocardia, which show acid-fastness on ZiehlNeelsen or similar stains. Other organisms may need to be identified by their growth in special media, or by other techniques, such as serology.

What stain shows better test results on Norcadia bacteria?
Answer:
ZiehlNeelsen