To the Editor:
There is a growing body of evidence that suggests that children have largely been spared of much of the morbidity associated with the ongoing SARS-CoV-2 pandemic 1,2 Over the last several weeks, however, there has been an increasing awareness and understanding of a hyperinflammatory shock syndrome that appears to mimic some aspects of Kawasaki disease in some pediatric patients. [3] [4] [5] It has been termed multisystem inflammatory syndrome in children (MIS-C), and clinically this syndrome presents as a constellation that may include high-spiking fevers, abdominal pain, rash, conjunctivitis, peripheral edema, hypotension, and cardiac dysfunction. [3] [4] [5] The pathophysiology that underlays this syndrome has yet to be elucidated; however, the overlap in clinical presentation with Kawasaki disease may suggest a commonality. Work to further clarify this relationship is ongoing. What is well described is an association between this syndrome and recent infection with SARS-CoV-2. 3 Given the clinical overlap of MIS-C with Kawasaki disease, there has been an effort to identify a common pathway that may be involved in the two entities. While the role that neutrophils play in the development of Kawasaki disease continues to be clarified, it is described in the literature that higher neutrophil to lymphocyte ratios (NLRs) may portend an increased risk of resistance to treatment with intravenous immunoglobulin as well as development of coronary aneurysms 6, 7 Indeed, there is also evidence that suggests that higher NLRs in patients with COVID-19 are associated with increased levels of inflammation and clinical severity. 8 Neutrophils in Kawasaki disease have been observed to exhibit morphologic changes, including vacuolization and toxic granulation, a phenomenon observed in inflammatory states and sepsis. [9] [10] [11] Here, we report our observations from peripheral blood smears for three children for whom the pediatric hematology service at our institution was consulted for assistance in management of COVID-19related coagulopathy. During the course of their care, we reviewed the peripheral smears of these children and noted the changes described below. These children all met diagnostic criteria for MIS-C as outlined by the Centers for Disease Control and Prevention. 12 All were less than 21 years of age and presented with fever and evidence of a severe inflammatory process, including elevation in CRP, ferritin, and D-dimer (Table 1 ). All required admission for severe illness with multisystem organ involvement and all were found to have evidence of current or recent infection with SARS-CoV-2.
Baseline characteristics, clinical features of illness, and hematologic parameters are depicted in Table 1 . Cell counts shown in Table 1 represent peak counts prior to receipt of corticosteroids. NLRs were calculated using the ratio of absolute neutrophil count (ANC) to absolute lymphocyte count (ALC). Peak inflammatory markers represent maximal values resulted during the course of admission. The images shown in Figure 1 are from peripheral smears obtained prior to receipt of systemic corticosteroids.
Through review of hematologic parameters for these three patients with MIS-C, we make two observations. The first is that these children exhibited high NLRs, a phenomenon previously described in adult patients with severe courses of COVID-19 but also in children with Kawasaki disease. 6, 7 This is notable, given considerable overlap between SARS-CoV-2-associated MIS-C and atypical Kawasaki disease. We additionally observed the consistent finding of a left-shifted blood smear with prominent bandemia and presence of other early myeloid forms, including metamyelocytes and myelocytes. Prominent vacuolization and toxic granulation, along with the presence of Dohle bodies, was observed in the myeloid lineage. These findings have been described in severe inflammatory processes, sepsis, and, notably, Kawasaki disease. [9] [10] [11] Morphologic changes in peripheral blood smears have been reported recently in patients with COVID-19, 13, 14 however this has, to our knowledge, not been described in the pediatric population. These 