aakarsha v. rao
Graduate Research Assistant at University of Louisville
phd student | graduate ra | bioinformatics | metagenomics | gut microbiome | metabolomics | python | matlab | cardinal | uofl
reinventing expanding skillset include statistical , computational analytical component bolster research interest metagenomics , metabolomics , epigenetics , molecular biology immunology , pursuing phd degree bioinformatics . coding skill : python , c++ , excel bioinformatics tool : picrust2 , qiime2 , lefse soft skill : critical thinking , widely adaptive , quick learner , efficient problem solving ability take pride student life , always eager learn experiment ! nothing keep driven towards goal desire versatile self-sufficient biomedical research . looking forward connecting world !
University of Louisville
https://www.linkedin.com/company/university-of-louisville
Graduate Research Assistant
Louisville, Kentucky, United States
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University of Wisconsin-Madison
https://www.linkedin.com/company/uwmadison
Research Assistant
-- Examined Bromodomain inhibitions with JQ1 in the context of Head and Neck Squamous Cell Carcinoma. Independently designed, conducted and analyzed experiments to test the differences in response to JQ1 in two primary cell lines. 
--  Well versed in Mammalian Cell Culture, Protein Isolation, Western Blots and Flow Cytometry.
-- Was responsible for lab inventory management and administration of active protocols maintained in the lab.
-- Mentored undergraduate and graduate students to help design and brainstorm ideas for their projects.
Madison, Wisconsin Area
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University of Louisville
https://www.linkedin.com/company/university-of-louisville
Research Assistant
Worked on multiple NIH funded projects on Alcohol and Immune Suppression at The Division of Gastroenterology, Hepatology and Nutrition. My projects revolved around evaluating the effect of alcohol exposure (in vitro and ex-vivo) on various aspects:-
1. Human T-cell proliferation
2. Brain inflammation 
3. Epigenetic changes that dictate the changes in gene expression of certain classes of chemokines

Poster Presentation:
Presented at Research!Louisville 2017, University of Louisville, September 2017.
Title: Role of Promoter Associated Epigenetic Mechanisms In Alcohol- and HIV-Induced Immune Suppression
Authors: S. Ghare, PhD1, P. Peyrani, M.D.2, A. Vijaya Kumar, M.S.1, S. Joshi-Barve, PhD1, C. McClain, M.D.1,3, and S. Barve, PhD1.
1. Division of Gastroenterology, Hepatology and Nutrition, 2. Division of Infectious Diseases, Department of Medicine, University of Louisville; 3. Robley Rex VA Med Center, University of Louisville Alcohol Research Center

Participated in standardizing protocols and preparing SOPs for:
a) Chromatin preparation using micrococcal nuclease from alcohol treated THP-1 cells
b) Multi-color Flow Cytometry (BD FACS Canto II) to characterize human T-cell proliferation patterns, cell death assays (Annexin V and PI) and to characterize various immune cells from a mixed bag of cells from rat liver
Louisville, Kentucky Area
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University of Texas at Dallas
https://www.linkedin.com/company/university-of-texas-at-dallas
Graduate Student Research Volunteer
-Worked with ÄKTA pure system to perform protocols for Nickel, Anion Exchange and Gel Filtration chromatography
-Protein expression of FACT (FAcilitates Chromatin Transcription) chaperon and/or its domains Spt16, SSRP1 in bacterial DH5-alpha cells by transformation and in insect SF-21 cells by co-transfection
-Evaluated protein-protein interaction between FACT and histones H2A/H2B and H3/H4 by visualizing using Hi-throughput Interaction by Fluorescence Intensity (Hi-Fi) technique
Richardson, Texas
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Indian Institute of Science (IISc)
https://www.linkedin.com/company/indian-institute-of-science
Undergraduate Student Researcher
Studied gene expression patterns of novel genes - CALML5, SCRG1 and HORMAD1 in breast cancer tissue obtained from patients' biopsy samples.
-Processed tumor tissue samples by homogenization in liquid nitrogen.
-RNA isolation from samples processed and stored in TRI-zol
-qPCR to evaluate the gene expression patterns. 
-Introduction to data analysis on Graph Pad Prism.
Bengaluru Area, India
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Biocon
https://www.linkedin.com/company/biocon
Project Trainee
-Worked with senior scientists and researchers to design Monoclonal Antibodies (MAbs), novel and biosimilar by purifying protein expressed in CHO cell lines
-Purification techniques followed included High Performance Liquid Chromatography (HPLC), Size Exclusion Chromatography (SEC), Ion Exchange Chromatography (IEX)
-Inculcated the confidence to handle state of the art systems like Waters and Agilent independently, with minimal supervision
-Responsibilities: preparation of chromatography buffers, setting up the systems for running samples, analyzing results, record keeping of experimental protocols and results
Bangalore, India
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Bioinformatics
Doctor of Philosophy - PhD
University of Louisville
https://www.linkedin.com/school/university-of-louisville/
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Cell/Cellular and Molecular Biology
Master’s Degree
The University of Texas at Dallas
https://www.linkedin.com/school/university-of-texas-at-dallas/
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Biotechnology
Bachelor of Engineering (B.E.)
PES University
https://www.linkedin.com/school/pesuniversity/
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Epigenetic mechanisms underlying HIV-Infection induced susceptibility of CD4+ T cells to enhanced activation-induced FasL expression and cell death
Journal of Acquired Immune Deficiency Syndrome
177Lu-NM600 targeted radionuclide therapy extends survival in syngeneic murine models of triple-negative breast cancer.
Journal of Nuclear Medicine
http://jnm.snmjournals.org/content/61/8/1187.full.pdf+html
Scholarship: fully-funded admission to PhD program, University of Louisville
University of Louisville
Awarded scholarship for 100% funding (tuition + research + Graduate RA)
Gifted Student Development Program (2010-2014)
PES University
Distinction Awards and Merit certificates through 8 semesters of engineering in Biotechnology
Biochemistry
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Cell Biology
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Immunology
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Molecular Biology
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Neuropathology
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Proteomics
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Quantitative Biology
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RNA Biology
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Data Mining
CSE-632
Data Structures
CSE302
Design and Analysis of Algorithms
CSE-619
Introduction to Bioinformatics
CSE660
Probability
PHST-661
Python and Data Analytics
CSE590
Downstream Process Technology
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Genetic Engineering
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Heat and Mass Transfer
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Microbiology
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Upstream Process Technology
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RNA-Sequencing: Comparison of Clustering algorithms on RNA-Sequencing Cancer Dataset
Designed clustering algorithms (K-means, Hierarchical and BIRCH) to group 800+ samples based on the characteristic differential gene expression patterns
Tasks: Data Preprocessing and Descriptive Data Mining 
Packages: SciKit Learn, Pandas DataFrames, NumPy, Matplotlib
Machine Learning approach to Prediction of Credit Card Loan Outcomes
Trained 4 machine learning algorithms: Naive Bayes, Logistic Regression, Linear SVC, Decision Trees to predict probability of an applicant to be "at risk" or "at no risk" of delinquency.
Tasks accomplished in a nutshell: Exploratory Data Analysis, Data Preprocessing, Model Training, Analyzing performance of models using F1-score and Area under ROC curve
Packages: Pandas DataFrames, NumPy, SciKit learn, MatplotLib
Data Mining: Perceptions of GameStop controversy on Reddit and Twitter
Elucidated the influence of social media on perceptions of GameStop controversy. 
Data Preprocessing (Feature Selection, Feature Extraction, Data Normalization) Data Visualization
Packages: Pandas DataFrames, NumPy, MatplotLib
Pairwise Sequence Alignment, Expectation Maximization Algorithm and Implementation of Distance Matrices
-- Object Oriented Programming design for evaluating pairwise sequence alignment pattern and scores
-- Implementation of the Expectation-Maximization statistical model for Multiple Sequence Alignment in Python 
-- Comparison, Evaluation and Implementation of Distance Matrices in Python
Social Media Data Mining and Analysis: Natural Language Processing, Sentiment Analysis and Data Interpretation
Mined social media (Twitter) to collate tweets related to particular topics, preprocessed them, analyzed tweet text for sentiment and tonality, trained and tested unsupervised and supervised machine learning models to predict sentiment of tweets, interpreted and depicted data using graphs and word clouds. 
Packages used:
-- Social media mining: Tweepy library - Twitter API Trends; geopy library - geocoding 
-- Natural Language Processing: TextBlob, StaCy, nltk module
-- Data Interpretation: MatPlotlib, Pandas series and DataFrames, NumPy 
-- Machine Learning: SciKit learn package
Effect of BET bromodomain inhibition by JQ1 on the differences in proliferation and sustenance of HPV-positive Head and Neck Squamous Cell Carcinoma (HNSCC) cell lines.
Analysis of phenotypic outcomes of BET bromodomain inhibition in the context of Head and Neck Squamous Cell Carcinoma (HNSCC)
Role of Promoter Associated Epigenetic Mechanisms In Alcohol- and HIV-Induced Immune Suppression
Poster Presentation:
Presented at Research!Louisville 2017, University of Louisville, September 2017.
Title: Role of Promoter Associated Epigenetic Mechanisms In Alcohol- and HIV-Induced Immune Suppression
Authors: S. Ghare, PhD1, P. Peyrani, M.D.2, A. Vijaya Kumar, M.S.1, S. Joshi-Barve, PhD1, C. McClain, M.D.1,3, and S. Barve, PhD1.
1. Division of Gastroenterology, Hepatology and Nutrition, 2. Division of Infectious Diseases, Department of Medicine, University of Louisville; 3. Robley Rex VA Med Center, University of Louisville Alcohol Research Center
Gene Expression Studies in Breast Cancer
The project carried out at the "Indian Institute of Science" Bangalore, India, mainly centered around evaluating the expression of three novel genes namely CALML5 (Calmodulin Like 5), SCRG1 (Stimulator of Chondrogensis 1) and HORMAD1 (HORMA Domain Containing 1) by comparing their expression with TBP (TATA Box Binding Protein) - a housekeeping gene using quantitative Real Time PCR. The other experiments which were performed include RNA isolation following standard protocol, subsequent conversion of RNA to cDNA by reverse transcription and PCR (Polymerase Chain Reaction) of the cDNA.
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Human Subjects and HIPAA Research
40591784
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CITI Program
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HIPAA Privacy
43341490
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CITI Program
https://www.linkedin.com/in/priyankabhandary
Priyanka Bhandary
Ph.D. candidate | Bioinformatics and Computational Biology | Machine learning | Bioinformatics Scientist | NGS | RNA-seq |
Ames, IA
https://ie.linkedin.com/in/bhairavi-bk-437a60166
Bhairavi BK
MSc Diagnostics and Precision Medicine Graduate
Dublin
https://es.linkedin.com/in/arnau-peris-cuesta-a084781b6
Arnau Peris-Cuesta
Computational Biology / Bioinformatics
Alcoy
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Prachi Prakash Khekare
Research Assistant
Santa Clara, CA
https://ch.linkedin.com/in/suraj-balakrishna-9aa1b793
Suraj Balakrishna
Researcher at University Hospital Zürich
Zurich
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Monica Bautista
Doctoral Student at The University of Texas at Dallas
Forney, TX
https://www.linkedin.com/in/shruti-eswar
Shruti Eswar
Bioinformatician pursuing a doctorate degree | Cincinnati Children's Hospital | Northeastern Alumna
Cincinnati, OH
https://www.linkedin.com/in/swethakasetty
Swetha Kasetty
PhD Candidate at Dartmouth College
White River Junction, VT
https://www.linkedin.com/in/keerthananprabhu
Keerthana Nagesh Prabhu
Bioinformatics Associate at Sound Agriculture
Berkeley, CA
https://www.linkedin.com/in/sneha-mohan-7a912117b
Sneha Mohan
Neuroscience PhD Student at University of Georgia
Athens, GA
https://www.linkedin.com/in/sarada-preeta-kalainayakan
Sarada Preeta Kalainayakan, Ph.D.
Senior Medical Writer at Real Chemistry | Medical Affairs | Oncology | Immunooncology
Philadelphia, PA
https://in.linkedin.com/in/natasha-miller-32627a65
NATASHA miller
Student at sophia college
Bengaluru
https://www.linkedin.com/in/priyanarayananbinf
Priya Narayanan
Associate Scientist II, Bioinformatics Analyst at AbbVie (Genomics Research Center)
San Francisco Bay Area
https://in.linkedin.com/in/manasa-m-852489221
Manasa M
--
Bengaluru
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Zheng Zhou, Ph.D, PMP
Research Assistant | Biosciences Ph.D. | Seeking jobs in healthcare consulting or life sciences project management
Centreville, VA
https://www.linkedin.com/in/shobanajayapalan
Shobana Jayapalan
Research Associate-II| BioAssay Services & PD | Molecular Biologist| Cell & Gene Therapy Enthusiast
Greater Houston
https://www.linkedin.com/in/parmithakker
Parmi Thakker
Senior Research Associate at Ring Therapeutics
Boston, MA
https://www.linkedin.com/in/harit7
Harit Vishwakarma
PhD Student @ UW-Madison
Madison, WI
Divya Anantsri
      
          



            
              
        
              
  

    
        Aakarsha is a very hardworking and talented molecular biologist. I had the privilege of collaborating with her on various class projects and practical classes in immunology and molecular biology and found her enthusiasm for science to be very inspiring. She is definitely a team player and has an inquisitive mind. I strongly believe that her drive for scientific research strong technical skills will make her a great fit for a biotechnology industry position. 
Congratulations to Mengwei Jiang for receiving the AASLD Early Career Investigator Award in Basic Science! 👏🎉
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Chantal Vidal, Ph.D., a post-doc in Dr. Prasanna Alluri's lab, has been selected as a UT Southwestern Provost’s Initiative for Diverse Emerging…
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Our new study is rolling-out!!#MiaGB Study, #aging, #geriatrics #brainhealth, #dementia, #alzheimer #diet, #microbiome#probiotics, #postbiotics…
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Liked by Aakarsha V. Rao
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MCB/MCO Alumni
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Changing FDA Regulatory landscape in the United States
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Society for Industrial Microbiology and Biotechnology  (SIMB)
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MD Anderson Cancer Center
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Pharmaceutical, Biotech, Medical & Healthcare Network: Business, Jobs, Events & News
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Genomics Jobs
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Bioconductor R Packages
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UT Dallas JSOM Career Management Center
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UT Dallas - UTD - University of Texas at Dallas
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Cell and Molecular Biology
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