Aaron Almeida
Manager: Manufacturing Process Optimization at Catalent Pharma Solutions
Manager: Manufacturing Process Optimization at Catalent Pharma Solutions
Pharmaceutical manufacturing leader with core technical competencies in solid-phase chemistry, cell culture, chromatography and filtration based purification methods, bioconjugation chemistry and structural/conformational analysis.
Experienced in product development from basic research with in vitro and in vivo testing, target optimization, process development, tox batch production and technology transfer around both biologic and synthetic pharmaceutical agents. 
Experienced creating and leading client facing technical groups focused around process data monitoring and optimizing manufacturing operations.
Catalent Pharma Solutions
https://www.linkedin.com/company/catalent-pharma-solutions
Manager: Manufacturing Process Optimization
Helped design, develop and roll out new group within pharmaceutical manufacturing operations. 
Technical project leadership around tech transfer, batch production records, process monitoring, process data trending, deviation investigation, CAPA, and optimization through process modeling with a focus on wholistic project analytics and client management

CI group leadership around standard work flow, standard process analytics strategies using large data sets, process automation and process modeling.
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Catalent Pharma Solutions
https://www.linkedin.com/company/catalent-pharma-solutions
Group Leader/Supervisor: Downstream Process Development
Group and lab management focused around tech transfer to GMP manufacturing. Optimize platform development strategies for rapid process identification and procedural optimizations for efficient tech transfer. 
SOP and BPR document drafting and review around GMP manufacturing and development activities.
Lab and inventory management around lean six sigma principles.
Manage programs and production runs with supply chain and procurement
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Catalent Pharma Solutions
https://www.linkedin.com/company/catalent-pharma-solutions
Downstream Process Development Scientist
Downstream Project Lead for programs in Process Development and Tech Transfer.
Manager of downstream support for programs in cell line development and upstream process development. 
Plan to Execute Pillar Lead for site rollout of the new inventory management system JD Edwards EnterpriseOne 9.1
Project Planner support for Process Development
and Supply Chain Lead for Continuous Improvement projects in Materials Management for PD and GMP MFG material supply
Middleton, WI
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University of Wisconsin-Madison
https://www.linkedin.com/company/uwmadison
Adjunct Professor: Applied Biotechnology Program
Course lecturer for ABT770: Product Development
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University of Wisconsin-Madison
https://www.linkedin.com/company/uwmadison
Honorary Fellow: Applied Biotechnology Program
Course Development for ABT 770: Product Development
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Arrowhead Research Corp.
https://www.linkedin.com/company/arrowhead-pharmaceuticals
Senior Scientist
Main chemistry lead for RNA interference (RNAi) targeting ligand discovery research from initial concept and chemistry design, pharmacokinetic and biodistribution optimizations to early stage tox batch production.

Established and managed in-house peptide synthesis facility with 3 full time employees. The group regularly used a combination of solution and solid-phase chemistry to generate non-traditional peptides with modifications that enabled conjugation, improved in vivo stability and/or improved biodistribution. Peptide synthesis followed by HPLC purification and lyophilization was regularly used to isolate the peptide on the single milligram to multigram batch scale size.
Madison, Wisconsin Area
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University of Wisconsin-Madison
https://www.linkedin.com/company/uwmadison
Graduate Student
As protein structure is intrinsically linked to the conformation that it adopts in solution, my research worked to experimentally identify how fundamental chemical features contribute to the stability of the parallel beta-sheet conformation. I regularly carried out non-traditional peptide synthesis in combination with solution phase chemistry to generate peptide oligomers (e.g. 10-30) of alpha-amino acid residues which contain multiple diacids and diamines incorporated into the peptide backbone. 
	Traditional biophysical strategies (Circular Dichroism, Analytical Ultracentrifugation) were used in coordination with NMR analysis (chemical shift and nOe based) to determine the conformation of the peptides. The nOe's would then be incorporated into molecular dynamics simulations to calculate the conformation of the peptides in solution.
Chemistry Department
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Organic Chemistry
Ph.D. Chemistry
University of Wisconsin-Madison
https://www.linkedin.com/school/uwmadison/
Under the supervision of Samuel H. Gellman
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Chemistry
Bachelors of Science
Union College
https://www.linkedin.com/school/union-college/
Under the supervision of Joanne D. Kehlbeck
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HIF2α targeted RNAi therapeutic inhibits clear cell renal cell carcinoma
Molecular cancer therapeutics
http://mct.aacrjournals.org/content/early/2017/10/27/1535-7163.MCT-17-0471
New Charge-bearing Amino Acid Residues That Promote Beta-Sheet Secondary Structure
J. Am. Chem. Soc
Proteinogenic amino acid residues that promote β-sheet secondary structure are hydrophobic (e.g., Ile or Val) or only moderately polar (e.g., Thr). The design of peptides intended to display β-sheet secondary structure in water typically requires one set of residues to ensure conformational stability and an orthogonal set, with charged side chains, to ensure aqueous solubility and discourage self-association. Here we describe new amino acids that manifest substantial β-sheet propensity, by virtue of β-branching, and also bear an ionizable group in the side chain.
http://pubs.acs.org/doi/abs/10.1021/ja510265e
Evaluation of a Cyclopentane-Based γ-Amino Acid for the Ability to Promote α/γ-Peptide Secondary Structure
Journal of Organic Chemistry
We report the asymmetric synthesis of the γ-amino acid (1R,2R)-2-aminomethyl-1-cyclopentane carboxylic acid (AMCP) and an evaluation of this residue’s potential to promote secondary structure in α/γ-peptides. Simulated annealing calculations using NMR-derived distance restraints obtained for α/γ-peptides in chloroform reveal that AMCP-containing oligomers are conformationally flexible. However, additional evidence suggests that an internally hydrogen-bonded helical conformation is partially populated in solution. From these data, we propose characteristic NOE patterns for the formation of the α/γ-peptide 12/10-helix and discuss the apparent conformational frustration of AMCP-containing oligomers
http://pubs.acs.org/doi/abs/10.1021/jo401501g?prevSearch=%255BContrib%253A%2Bgiuliano%252C%2Bm.w.%255D&searchHistoryKey=
Parallel β-Sheet Vibrational Couplings Revealed by 2D IR Spectroscopy of an Isotopically Labeled Macrocycle: Quantitative Benchmark for the Interpretation of Amyloid and Protein Infrared Spectra.
J. Am. Chem. Soc
Authors: Woys, A., Almeida A.M., Wang L., Chiu C-C, McGovern, M.P. de Pablo J.J., Skinner, J.L., Gellman, S.H., Zanni, M.T.
http://pubs.acs.org/doi/abs/10.1021/ja3074962
Parallel β-Sheet Secondary Structure Is Stabilized and Terminated by Interstrand Disulfide Cross-Linking
J. Am. Chem. Soc.
Disulfide bonds between Cys residues in adjacent strands of parallel β-sheets are rare among proteins, which suggests that parallel β-sheet structure is not stabilized by such disulfide cross-links. We report experimental results that show, surprisingly, that an interstrand disulfide bond can stabilize parallel β-sheets formed by an autonomously folding peptide in aqueous solution. NMR analysis reveals that parallel β-sheet structure is terminated beyond the disulfide bond, which causes deviation from the extended backbone conformation at one of the Cys residues.
http://pubs.acs.org/doi/abs/10.1021/ja208856c
Impact of Strand Length on the Stability of Parallel-β-Sheet Secondary Structure
Wiley/ Angewandte Chemie
Authors: Dr. Felix Friere (+), Aaron M. Almeida(+), John D. Fisk, Jay D. Steinkruger, Prof. Samuel H. Gellman 
These Authors contributed equally

Molecular design can be used to investigate whether the parallel-β-sheet secondary structure is subject to length-dependent cooperative stabilization along the strand direction. Extending strands by adding high-propensity residues leads to a steady increase in parallel-β-sheet stability, but inclusion of low-propensity residues can negate this stabilization.
http://onlinelibrary.wiley.com/doi/10.1002/anie.201102986/abstract
Helix formation in preorganized beta/gamma-peptide foldamers: hydrogen-bond analogy to the alpha-helix without alpha-amino acid residues.
J. Am. Chem. Soc.
Authors: Guo L, Almeida AM, Zhang W, Reidenbach AG, Choi SH, Guzei IA, Gellman SH.

We report the first high-resolution structural data for the beta/gamma-peptide 13-helix (i,i+3 C=O...H-N H-bonds), a secondary structure that is formed by oligomers with a 1:1 alternation of beta- and gamma-amino acid residues. Our characterization includes both crystallographic and 2D NMR data. Previous studies suggested that beta/gamma-peptides constructed from conformationally flexible residues adopt a different helical secondary structure in solution. Our design features preorganized beta- and gamma-residues, which strongly promote 13-helical folding by the 1:1 beta/gamma backbone.
http://pubs.acs.org/doi/abs/10.1021/ja103233a
Stereospecific synthesis of conformationally constrained gamma-amino acids: new foldamer building blocks that support helical secondary structure,
J. Am. Chem. Soc.
Authors : Li Guo, Yonggui Chi, Aaron M. Almeida, Ilia A. Guzei, Brian K. Parker and Samuel H. Gellman

A highly stereoselective synthesis of novel cyclically constrained γ-amino acid residues is presented. The key step involves organocatalytic Michael addition of an aldehyde to 1-nitrocyclohexene. After aldehyde reduction, this approach provides optically active β-substituted δ-nitro alcohols (96−99% ee), which can be converted to γ-amino acid residues with a variety of substituents at the α position. We have used these new building blocks to prepare α/γ-peptide foldamers that adopt a specific helical conformation in solution and in the solid state.
http://pubs.acs.org/doi/abs/10.1021/ja907233q
A γ-Amino Acid that Favors 12/10-Helical Secondary Structure in α/γ-Peptides
http://pubs.acs.org/doi/abs/10.1021/ja5076585
Chromatographic capture of cells to achieve single stage clarification in recombinant protein purification
https://aiche.onlinelibrary.wiley.com/doi/10.1002/btpr.3227
ALPHA-V BETA-6 INTEGRIN LIGANDS AND USES THEREOF
20190248832
http://www.freepatentsonline.com/y2019/0248832.html
Compositions and Methods for Inhibiting Gene Expression of Hif2alpha
20180230470
https://patents.justia.com/patent/20180230470
Compositions and Methods for Inhibiting Gene Expression of LPA
20180195070
https://patents.justia.com/patent/20180195070
Disulfide-containing alkyne linking agents
10,047,361
https://patents.justia.com/patent/10047361
https://www.linkedin.com/in/kimberly-s-8aaa21115
Kimberly S.
Research Specialist at AgraFilms
Greater Madison Area
https://www.linkedin.com/in/lucas-j-king
Lucas King
Supervisor, cGMP Downstream/mRNA Therapeutic Biomanufacturing  at Catalent Pharma Solutions
Madison, WI
https://www.linkedin.com/in/ethan-grant-3787b9138
Ethan Grant
Senior Scientist I at Catalent Pharma Solutions
Greater Madison Area
https://www.linkedin.com/in/kevin-jose-46724857
Kevin Jose
Sr. Group Leader at Catalent
Greater Madison Area
https://www.linkedin.com/in/connor-rupke-533907193
Connor Rupke
--
Raleigh, NC
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Kevin Vergenz
Los Angeles, CA
Los Angeles Metropolitan Area
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Bhaumik Vyas
Senior Process Engineer at Catalent Pharma Solutions
Madison, WI
https://www.linkedin.com/in/eric-york-6a9b3929
Eric York
Head of Data Visualization @ Wayfair
Watertown, MA
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Jennifer Finta
Process Engineer at Catalent Pharma Solutions
Madison, WI
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Shannon M. Flahive
Venture Capital Investor | Business Strategist
Denver Metropolitan Area
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Radha Patel
VP, Retail @ Whoop
Boston, MA
https://www.linkedin.com/in/ronald-zuniga-3724921b
Ronald Zuniga
Supervisor Downstream Biomanufacturing, Fujifilm. College Station, Texas at FUJIFILM Diosynth Biotechnologies
Seguin, TX
https://www.linkedin.com/in/darren-thiel-612797171
Darren Thiel
Quality Assurance Specialist at Catalent Pharma Solutions
Greater Madison Area
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Katie Berth
Manager, flex MS&T at Catalent Pharma Solutions
Madison, WI
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Joshua Maurer
Quality Assurance Supervisor at Catalent Pharma Solutions
Greater Madison Area
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William Dunmire
Recreations Director at Valley Lo Club
Milwaukee, WI
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Emily Schirmer
Senior Director Process Development at Catalent Pharma Solutions
Madison, WI
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Jitendra Mishra, PhD, MBA
Associate Director
Berkeley, CA
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Guohua Liang
Senior Manager, API Manufacturing and Process Development at Avedro, Inc.
Acton, MA
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Aaron Almeida
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Solicitor
Greater Adelaide Area
Aaron Almeida
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SAP Supply Chain Senior Consultant at Deloitte US-India | IBM Alumnus | NITK Alumnus
Bengaluru
Aaron Almeida
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Regional Data Analyst at TikTok
United Arab Emirates
Aaron Almeida
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Associate Consultant at Oracle Financial Services Software Limited
Mumbai
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Wisconsin Alumni (Official)
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Oligonucleotide and Peptide Development Professionals
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Union College Alumni Group
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Samuel H. Gellman Group Alumni
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Midwest Life Science Network (LSN)
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University of Wisconsin–Madison
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TIDES (Peptides & Oligonucleotides) Community
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Organic Chemists in Drug Discovery
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Peptide Synthesis
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