The paradox of micronutrients : in vitro and human studies

Many essential micronutrients found in fruits and vegetables exhibit antioxidant
properties, protecting against oxidation and oxidative stress. Their ability to react with
electrons also enables them to act as pro-oxidants, causing damage opposed to
preventing it. While fruit and vegetables rich in micronutrients generally protect against
oxidative damage and related diseases, supplementation studies with micronutrients
have not been verified to have the same health beneficial effect. In fact, even harmful
consequences have been seen. The reason for the ambiguous results is still not fully
understood and this thesis investigates some of the factors believed to be involved. The
focus is on the effect of micronutrients on DNA lesions, potential contributors to cancer
development. The thesis takes you into chemical properties of micronutrients (paper I
and II), it also explores their health effects in two human studies (paper III and IV).




When investigating pro-oxidant properties of thirteen micronutrients in this thesis, two
of them stood out oxidising the DNA nucleoside deoxyguanosine in a dose-dependent
manner – namely vitamin A and vitamin C. Compounds permitted in supplements as
either ‘vitamin A’ (four compounds) or ‘vitamin C’ (five compounds) also differed in
their potencies to act as pro-oxidants. The combination of vitamin C and copper
particularly stood out, inducing oxidation to pure deoxyguanosine, but also to the DNA
of cells in culture, a potential concern since both are commonly found in multivitamin
supplements.




In an observational study, the impact of folate/folic acid status, intake or genes on DNA
lesions (uracil misincorporation, oxidative DNA lesions, DNA breaks) in mononuclear
cells of 36 infertile women was explored. Folate and related parameters were found not
to correlate with DNA lesions in these women with adequate folate status. Our results
add to the carefully accumulating evidence indicating that, even though folate status
has been associated with DNA lesions, the effect seems to be more evident in subjects
with folate deficiency. An increased interest has been drawn towards micronutrient
supplements imitating the natural content of fruit, vegetables and berries, as for
example with berry extracts. This thesis report on a cross-over intervention study where
chronic kidney disease patients were administered a supplement containing oil extracts
from the sea buckthorn berry rich in micronutrients and fatty acids. An eight-week
administration did not affect their levels of DNA lesions (oxidative DNA lesions, DNA
breaks) in minor salivary glands, nor mouth dryness or disease specific parameters.




Taken together, the results within this thesis add to the somewhat confusing evidence
on micronutrient supplements and their properties and health effects. It emphasises that
each micronutrient has unique properties, some with strong pro-oxidant activity
dependent on factors in their immediate surrounding. The results also add to the
growing evidence of a lack of effect on health from micronutrient supplements. It
might be that supplementation with micronutrients provides best benefit in those with
initially low nutrient levels