pinecone/core-2020-05-10-deduplication huggingface dataset:
The component of Carica papaya seed toxic to A-aegypti and the identification of tegupain, the enzyme that generates it

As Aedes aegypti transmits the etiologic agents of both yellow and dengue fever; vector control is considered essential to minimise their incidence. the aim of this work was to identify the component of Carica papaya seed toxic to A. aegypti, and the identification of tegupain, the enzyme that generates it. Aqueous extracts (1%, w/v) of the seed tegument and cotyledon of C. papaya are not larvicidal isolately. However, a mixture of 17 mu g mL(-1) tegument extract and 27 mu g mL(-1) cotyledon extract caused 100% larval mortality in a bioassay. the mixture was no longer larvicidal after the tegument extract was pre-treated at 100 degrees C for 10 min. the enzyme tegupain efficiently hydrolysed the substrate Z-Phe-Arg-pNan (K-m 58.8 mu M, K-cat 28020 s(-1), K-cat/K-m 5 x 10(8) M-1 s(-1)), and its activity increased with 2 mM dithiothreitol (DTT), at 37 degrees C, pH 5.0. the chelating agent EDTA did not modify the enzyme activity. Inhibition of tegupain by cystatin (K-iapp 2.43 nM), E64 (3.64 nM, 83% inhibition), and the propeptide N-terminal sequence indicate that the toxic activity is due to a novel cysteine proteinase-like enzyme, rendered active upon the hydrolysis of a cotyledon component of C. papaya seeds. (c) 2013 Elsevier B.V. All rights reserved.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Federal de São Paulo, Dept Bioquim, BR-04044020 São Paulo, BrazilUniv Estadual Norte Fluminense, Biotechnol Lab, Campos Dos Goytacazes, RJ, BrazilUniversidade Federal de São Paulo, Dept Bioquim, BR-04044020 São Paulo, BrazilFAPESP: 2009/17058-6FAPESP: 2009/53766-5Web of Scienc
Stability of fluid flow in a Brinkman porous medium - a numerical study

The stability of fluid flow in a horizontal layer of Brinkman porous medium with fluid viscosity different from effective viscosity is investigated. A modified Orr-Sommerfeld equation is derived and solved numerically using the Chebyshev collocation method. The critical Reynolds number Rec, the critical wave number αc and the critical wave speed cc are computed for various values of porous parameter and ratio of viscosities. Based on these parameters, the stability characteristics of the system are discussed in detail. Streamlines are presented for selected values of parameters at their critical state.postprin
Artificial control of ZnO nanodots by ion-beam nanopatterning

The use of focused ion-beam (FIB) nanopatterning for manipulating self-assembled ZnO nanodots is described. Highly aligned ZnO-nanodot arrays with various periodicities (e.g., 750, 190, and 100 nm) on FIB-nanopatterned SiO[2]/Si substrates were prepared by metal-organic chemical-vapor deposition (MOCVD). The artificially assembled ZnO nanodots had an amorphous structure. Ga atoms incorporated into the surface areas of FIB-patterned nanoholes during FIB engraving were found to play an important role in the artificial control of ZnO, resulting in the production of ZnO nanodots on the FIB-nanopatterned areas. The nanodots evolved into single-crystalline dot clusters and rods with increasing MOCVD-growth time. In addition, microphotoluminescence measurements showed that the ZnO-nanodot arrays have low-dimensional quantum characteristics
Sets of finite perimeter and the Hausdorff-Gauss measure on the Wiener space

In Euclidean space, the integration by parts formula for a set of finite perimeter is expressed by the integration with respect to a type of surface measure. According to geometric measure theory, this surface measure is realized by the one-codimensional Hausdorff measure restricted on the reduced boundary and/or the measure-theoretic boundary, which may be strictly smaller than the topological boundary. In this paper, we discuss the counterpart of this measure in the abstract Wiener space, which is a typical infinite-dimensional space. We introduce the concept of the measure-theoretic boundary in the Wiener space and provide the integration by parts formula for sets of finite perimeter. The formula is presented in terms of the integration with respect to the one-codimensional Hausdorff-Gauss measure restricted on the measure-theoretic boundary
Regnase-1 and Roquin Regulate a Common Element in Inflammatory mRNAs by Spatiotemporally Distinct Mechanisms.

炎症がRNA分解により制御されるメカニズムを解明 -二つのブレーキが炎症を巧妙にストップする-. 京都大学プレスリリース. 2015-05-22.Regnase-1 and Roquin are RNA binding proteins essential for degradation of inflammation-related mRNAs and maintenance of immune homeostasis. However, their mechanistic relationship has yet to be clarified. Here, we show that, although Regnase-1 and Roquin regulate an overlapping set of mRNAs via a common stem-loop structure, they function in distinct subcellular locations: ribosome/endoplasmic reticulum and processing-body/stress granules, respectively. Moreover, Regnase-1 specifically cleaves and degrades translationally active mRNAs and requires the helicase activity of UPF1, similar to the decay mechanisms of nonsense mRNAs. In contrast, Roquin controls translationally inactive mRNAs, independent of UPF1. Defects in both Regnase-1 and Roquin lead to large increases in their target mRNAs, although Regnase-1 tends to control the early phase of inflammation when mRNAs are more actively translated. Our findings reveal that differential regulation of mRNAs by Regnase-1 and Roquin depends on their translation status and enables elaborate control of inflammation
Solar cycle variation of magnetic flux ropes in a quasi-static coronal evolution model

The structure of electric current and magnetic helicity in the solar corona is closely linked to solar activity over the 11-year cycle, yet is poorly understood. As an alternative to traditional current-free "potential field" extrapolations, we investigate a model for the global coronal magnetic field which is non-potential and time-dependent, following the build-up and transport of magnetic helicity due to flux emergence and large-scale photospheric motions. This helicity concentrates into twisted magnetic flux ropes, which may lose equilibrium and be ejected. Here, we consider how the magnetic structure predicted by this model-in particular the flux ropes-varies over the solar activity cycle, based on photospheric input data from six periods of cycle 23. The number of flux ropes doubles from minimum to maximum, following the total length of photospheric polarity inversion lines. However, the number of flux rope ejections increases by a factor of eight, following the emergence rate of active regions. This is broadly consistent with the observed cycle modulation of coronal mass ejections, although the actual rate of ejections in the simulation is about a fifth of the rate of observed events. The model predicts that, even at minimum, differential rotation will produce sheared, non-potential, magnetic structure at all latitudes.PostprintPeer reviewe
Extensive MIS 3 glaciation in southernmost Patagonia revealed by cosmogenic nuclide dating of outwash sediments.

The timing and extent of former glacial advances can demonstrate leads and lags during 2 periods of climatic change and their forcing, but this requires robust glacial chronologies. In 3 parts of southernmost Patagonia, dating pre-global Last Glacial Maximum (gLGM) ice limits 4 has proven difficult due to post-deposition processes affecting the build-up of cosmogenic 5 nuclides in moraine boulders. Here we provide ages for the Río Cullen and San Sebastián 6 glacial limits of the former Bahía Inútil-San Sebastián (BI-SSb) ice lobe on Tierra del Fuego 7 (53-54°S), previously hypothesised to represent advances during Marine Isotope Stages 8 (MIS) 12 and 10, respectively. Our approach uses cosmogenic 10Be and 26Al exposure 9 dating, but targets glacial outwash associated with these limits and uses depth-profiles and 10 surface cobble samples, thereby accounting for surface deflation and inheritance. The data 11 reveal that the limits formed more recently than previously thought, giving ages of 45.6 ka 12 (+139.9/-14.3) for the Río Cullen, and 30.1 ka (+45.6/-23.1) for the San Sebastián limits. These dates 13 indicate extensive glaciation in southern Patagonia during MIS 3, prior to the well-14 constrained, but much less extensive MIS 2 (gLGM) limit. This suggests the pattern of ice 15 advances in the region was different to northern Patagonia, with the terrestrial limits relating 16 to the last glacial cycle, rather than progressively less extensive glaciations over hundreds of 17 thousands of years. However, the dates are consistent with MIS 3 glaciation elsewhere in 18 the southern mid-latitudes, and the combination of cooler summers and warmer winters with 19 increased precipitation, may have caused extensive glaciation prior to the gLGM
High-resolution stochastic integrated thermal-electrical domestic demand model

This is an Open Access Article. It is published by Elsevier under the Creative Commons Attribution 4.0 Unported Licence (CC BY). Full details of this licence are available at: http://creativecommons.org/licenses/by/4.0/This paper describes the extension of CREST's existing electrical domestic demand model into an integrated thermal-electrical demand model. The principle novelty of the model is its integrated structure such that the timing of thermal and electrical output variables are appropriately correlated. The model has been developed primarily for low-voltage network analysis and the model's ability to account for demand diversity is of critical importance for this application. The model, however, can also serve as a basis for modelling domestic energy demands within the broader field of urban energy systems analysis. The new model includes the previously published components associated with electrical demand and generation (appliances, lighting, and photovoltaics) and integrates these with an updated occupancy model, a solar thermal collector model, and new thermal models including a low-order building thermal model, domestic hot water consumption, thermostat and timer controls and gas boilers. The paper reviews the state-of-the-art in high-resolution domestic demand modelling, describes the model, and compares its output with three independent validation datasets. The integrated model remains an open-source development in Excel VBA and is freely available to download for users to configure and extend, or to incorporate into other models
Quantum deformations of D=4 Euclidean, Lorentz, Kleinian and
  quaternionic o^*(4) symmetries in unified o(4;C) setting

We employ new calculational technique and present complete list of classical
$r$-matrices for $D=4$ complex homogeneous orthogonal Lie algebra
$\mathfrak{o}(4;\mathbb{C})$, the rotational symmetry of four-dimensional
complex space-time. Further applying reality conditions we obtain the classical
$r$-matrices for all possible real forms of $\mathfrak{o}(4;\mathbb{C})$:
Euclidean $\mathfrak{o}(4)$, Lorentz $\mathfrak{o}(3,1)$, Kleinian
$\mathfrak{o}(2,2)$ and quaternionic $\mathfrak{o}^{\star}(4)$ Lie algebras.
For $\mathfrak{o}(3,1)$ we get known four classical $D=4$ Lorentz $r$-matrices,
but for other real Lie algebras (Euclidean, Kleinian, quaternionic) we provide
new results and mention some applications.Comment: 13 pages; typos corrected. v3 matches version published in PL
Writing a skyrmion on multiferroic materials

This paper reports on a theoretical proposal for electrical creation of
magnetic skyrmions on a thin-film specimen of a multiferroic chiral magnet by
local application of an electric field, instead of an electric current, via an
electrode tip. This method can be traced back to the mutual coupling between
skyrmion spins and the electric polarizations in multiferroics, and represents
a unique technique for use in potential skyrmion-based memory devices without
Joule-heating losses.Comment: 5 pages, 4 figure
Paths of zeros of analytic functions describing finite quantum systems

Quantum systems with positions and momenta in Z(d), are described by the d
zeros of analytic functions on a torus. The d paths of these zeros on the
torus, describe the time evolution of the system. A semi-analytic method for
the calculation of these paths of the zeros, is discussed. Detailed analysis of
the paths for periodic systems, is presented. A periodic system which has the
displacement operator to a real power t, as time evolution operator, is
studied. Several numerical examples, which elucidate these ideas, are
presented
Time-dependent Aharonov-Bohm effect on the noncommutative space

We study the time-dependent Aharonov-Bohm effect on the noncommutative space.
Because there is no net Aharonov-Bohm phase shift in the time-dependent case on
the commutative space, therefore, a tiny deviation from zero indicates new
physics. Based on the Seiberg-Witten map we obtain the gauge invariant and
Lorentz covariant Aharonov-Bohm phase shift in general case on noncommutative
space. We find there are two kinds of contribution: momentum-dependent and
momentum-independent corrections. For the momentum-dependent correction, there
is a cancellation between the magnetic and electric phase shifts, just like the
case on the commutative space. However, there is a non-trivial contribution in
the momentum-independent correction. This is true for both the time-independent
and time-dependent Aharonov-Bohm effects on the noncommutative space. However,
for the time-dependent Aharonov-Bohm effect, there is no overwhelming
background which exists in the time-independent Aharonov-Bohm effect on both
commutative and noncommutative space. Therefore, the time-dependent
Aharonov-Bohm can be sensitive to the spatial noncommutativity. \draftnote{The
net correction is proportional to the product of the magnetic fluxes through
the fundamental area represented by the noncommutative parameter $\theta$, and
through the surface enclosed by the trajectory of charged particle.} More
interestingly, there is an anti-collinear relation between the logarithms of
the magnetic field $B$ and the averaged flux $\Phi/N$ (N is the number of
fringes shifted). This nontrivial relation can also provide a way to test the
spatial noncommutativity. For $B\Phi/N\sim 1$, our estimation on the
experimental sensitivity shows that it can reach the $\rm 10GeV$ scale. This
sensitivity can be enhanced by using stronger magnetic field strength, larger
magnetic flux, as well as higher experimental precision on the phase shift.Comment: 12 pages, 1 figure; v2, accepted version by PL
Preoperative predictive factors of aneurysmal regression using the reporting standards for endovascular aortic aneurysm repair.

International audienceBACKGROUND: Aneurysmal regression is a reliable marker for long-lasting success after endovascular aneurysm repair (EVAR). The aim of this study was to identify the preoperative factors that can predictably lead to aneurysmal sac regression after EVAR, according to the reporting standards of the Society for Vascular Surgery and the International Society of Cardiovascular Surgery (SVS/ISCVS). METHODS: From 199 patients treated by EVAR between 2000 and 2009, 164 completed computed tomography angiographies and duplex scan follow-up images were available. All computed tomography angiographies for enrolled patients in this retrospective study were analyzed with Endosize software (Therenva, Rennes, France) to provide spatially correct 3-dimensional data in accordance with SVS/ISCVS recommendations. Anatomic parameters were graded according to the relevant severity grades. A severity score was calculated at the aortic neck, the abdominal aortic aneurysm, and the iliac arteries. Clinical and demographic factors were studied. Patients with aneurysmal regression >5 mm were assigned to group A (mean age, 71.4 ± 8.9 years) and the others to group B (76.3 ± 8.3 years). RESULTS: Aneurysmal regression occurred in 66 patients (40.2%; group A). Univariate analyses showed smaller severity scores at the aortic neck (P = .02) and the iliac arteries (P = .002) in group A and calcifications and thrombus were less significant at the aortic neck (P = .003 and P = .02) and at the iliac arteries (P = .001 and P = .02), and inferior mesenteric artery patency was less frequent (68.2% vs 82.7%, P = .04). Two multivariate analyses were done: one considered the scores and the other the variables included in the scores. In the first, the patients of group A were younger (P = .002) and aortic neck calcifications were less significant (P = .007). In the second, group A patients were younger (P < .001) and the aortic neck scores were smaller (P = .04). There was no difference between the two groups in the type of implanted endoprosthesis or in the follow-up (group A: 46.4 ± 24 months; group B: 47.2 ± 22 months; P = .35). CONCLUSIONS: In this study, the young age of the patients and their aortic neck quality, in particular the absence of neck calcification, appear to have been the main factors affecting aneurysm shrinkage, such that they represent a target population for the improvement of EVAR results
Hα Moreton waves observed on December 06, 2006

We present high temporal resolution observations of a Moreton wave event detected with the Hα Solar Telescope from  Argentina (HASTA) in the Hα line 656.3nm, on December 6th, 2006.  Aims. The aim is to contribute to the discussion about the nature and triggering mechanisms of Moreton wave events.  Methods. We describe the HASTA telescope capabilities and the observational techniques developed. We carry out a detailed analysis  of the radiant point as probable origin of the event. There are different radiant point determinations depending on non?discernible  interpretations of the observations. We use a 2D reconstruction of the HASTA and the corresponding TRACE observations, together  with conventional techniques, to analyse the coronal nature of the phenomenon.  Results. Chromosphere: The kinematic parameters of the Moreton wave event, associated with a X6.5 flare, are determined. Corona:  Small-scale ejecta and the winking of remote filaments are analysed to discuss the type of coronal MHD wave that can originate the  observed patterns in Hα images and its probable origin. The results strongly depend on the radiant point determination and motion.  Conclusions. The detailed analysis of the Moreton wave event favors the hypothesis that the phenomenon can be described as the  chromospheric imprint of a single fast coronal shock whose origin could be related to a flare pressure pulse, although a CME origin  can not be discarded.Fil: Francile, Carlos Natale. Universidad Nacional de San Juan. Facultad de Ciencias Exactas Físicas y Naturales. Departamento de Informática. Observat.astron. Felix Aguilar; ArgentinaFil: Costa, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto de Astronomía Teórica y Experimental; ArgentinaFil: Elaskar, Sergio Amado
All AdS7 solutions of type II supergravity

In M-theory, the only AdS7 supersymmetric solutions are AdS7 × S 4 and its orbifolds. In this paper, we find and classify new supersymmetric solutions of the type AdS7 × M 3 in type II supergravity. While in IIB none exist, in IIA with Romans mass (which does not lift to M-theory) there are many new ones. We use a pure spinor approach reminiscent of generalized complex geometry. Without the need for any Ansatz, the system determines uniquely the form of the metric and fluxes, up to solving a system of ODEs. Namely, the metric on M 3 is that of an S 2 fibered over an interval; this is consistent with the Sp(1) R-symmetry of the holographically dual (1,0) theory. By including D8 brane sources, one can numerically obtain regular solutions, where topologically M 3 ≅ S 3
Indigenous yeast communities in the environment of  ‘Rovello  bianco’’ grape variety and their use in commercial white wine fermentation

The indigenous yeast communities associated \ud
with several vineyard habitats were analysed. Wild yeasts \ud
were isolated, differentiated at strain level and identified. \ud
A phylogenetic tree based on partial 26S rRNA genes \ud
was constructed. The strains were characterized and the \ud
indigenous Saccharomyces cerevisiae GR1 was then used \ud
to carry out a vinification process and compared with a \ud
commercial yeast. Wines obtained were subjected to \ud
chemical and sensory analysis. The comparison between \ud
the two products highlighted differences due to the fer- \ud
menting strains employed. The vineyard environment \ud
was found to strongly influence the composition of \ud
yeast communities, thus, confirming the theory of ‘terr- \ud
oir’ on the expression of wines. Moreover, vineyard \ud
inhabiting birds were in part responsible for the dis- \ud
semination of fermentative yeasts during their feedin
Indirect co-cultures of stem cells with chondrocytes for cartilage tissue engineering using PCL electrospun nanofiber meshes

Publicado em "Journal of Tissue Engineering and Regenerative Medicine", vol. 7, supp. 1 (2013)Mesenchymal Stem Cells (MSCs) have been recognized for their ability
to differentiate into cells of different tissues such as bone, cartilage or
adipose tissue, and therefore might be of interest for potential therapeutic
strategies. These cells are induced to differentiate by growth factors
supplementation in culture medium that will trigger differentiation
in the desired cell type. Chondrocytes are responsible for maintaining
the extracellular matrix (ECM) integrity of articular cartilage. Chondrocytes
have been shown to release growth factors that can ultimately
induce chondrogenic differentiation of undifferentiated cells, for example
MSCs. It is well known that chondrocytes tend to de-differentiate
when in 2D culture, losing their ability to produce a rich ECM. In this
process occurs a shift from collagen type II production to collagen type
I, among other factors, giving rise to a fibrocartilage tissue. In order to
overcome this problem, several tissue engineering strategies have been
proposed, involving different combinations of cells, including the use of
co-cultures. The present work presents a co-culture strategy using
human articular chondrocytes and stem cells (Wharton′s jelly stem
cells) for cartilage-like tissue production. We aimed at assessing the
paracrine effect that chondrocytes may have on stem cells by co-culturing
directly both cells on the two faces of NFM. The aim is to allow
communication of the two cells communities by soluble factors
released, but not having direct contact between them. Polycaprolactone
(PCL) nanofiber meshes (NFM) were produced by electrospinning. The
NFM were further placed into inserts (two in each insert) in order to
allow seeding each type of cells in opposite faces of the NFMs. Cells
were isolated from human samples collected at the local hospital, under
donors’ informed consent. After cells expansion, chondrocytes were
seeded on the top of the NFMs, whereas stem cells were seeded on the
bottom of the NFMs. Controls were performed by seeding chondrocytes
or stem cells in NFM. For evaluation of cell viability, proliferation and
distribution within the scaffolds, DNA, Alamar Blue and SEM methods
were used. Chondrogenic differentiation was evaluated using histological
staining, glycosaminoglycan quantification, qRT-PCR and immunolocalization.
Cells kept viable along the experiment. Stem cells were
able to over express cartilage related genes such as aggrecan, sox9 and
collagen type II when compared to the undifferentiated controls. Articular
chondrocytes induced the chondrogenic differentiation of stem
cells and ECM formation. The obtained results showed that this new
strategy enables the development of new therapies for cartilage repai