pinecone/core-2020-05-10-deduplication huggingface dataset:
Redox modulation of STIM-ORAI signaling.

STIM1 and ORAI1 constitute the core machinery of the ubiquitous store-operated calcium entry pathway and loss of function in these proteins is associated with severe immune and muscular disorders. Other isoforms-STIM1L, STIM2, ORAI2 and ORAI3 exhibit varied expression levels in different cell types along with several other interaction partners and thereby play different roles to facilitate, regulate and fine-tune the calcium entry. STIM proteins convey the Ca(2+) store-depletion message to the PM and thereby participate in refilling of the ER by physically interacting with the Ca(2+)-selective ORAI channels at the PM. STIM and ORAI are exposed to oxidative modifications in the ER, the cytosol, and at the cell surface, and redox-mediated alterations in STIM/ORAI coupling might contribute to autoimmune disorders and cancer progression. This review discusses the redox reactivity of cysteine residues in STIM and ORAI isoforms, focusing on the oxidative modifications of STIM and ORAI proteins by which STIM-ORAI signaling can be modulated
Measurement of the production cross section for a W boson and two b jets in pp collisions at √s = 7 TeV

The production cross section for a W boson and two b jets is measured using proton–proton collisions at √s = 7 TeV in a data sample collected with the CMS experiment at the LHC corresponding to an integrated luminosity of 5.0 fb−1. The W + bb events are selected in the W → μν decay mode by requiring a muon with transverse momentum pT &gt; 25 GeV and pseudorapidity |η| &lt; 2.1, and exactly two b-tagged jets with pT &gt; 25 GeV and |η| &lt; 2.4. The measured W + bb production cross section in the fiducial region, calculated at the level of final-state particles, is σ(pp → W + bb) × B(W → μν) = 0.53 ± 0.05 (stat.) ± 0.09 (syst.) ± 0.06 (theo.) ± 0.01 (lum.) pb, in agreement with the standard model prediction. In addition, kinematic distributions of the W + bb system are in agreement with the predictions of a simulation using MadGraph and pythia
Search for the standard model higgs boson in the decay channel h -> zz(()*()) -> 4l with the ATLAS detector

A search for the Standard Model Higgs boson in the decay channel H -> ZZ(()*()) l(+)l(-)l'l'(-), where l = e, mu, is presented. Proton-proton collision data at root s = 7 TeV recorded with the ATLAS detector and corresponding to an average integrated luminosity of 2.1 fb(-1) are compared to the Standard Model expectations. Upper limits on the production cross section of a Standard Model Higgs boson with a mass between 110 and 600 GeV are derived. The observed (expected) 95% confidence level upper limit on the production cross section for a Higgs boson with a mass of 194 GeV, the region with the best expected sensitivity for this search, is 0.99 (1.01) times the Standard Model prediction. The Standard Model Higgs boson is excluded at 95% confidence level in the mass ranges 191-197, 199-200 and 214-224 GeV
Measurement of the inclusive jet cross-section in proton-proton collisions at root s=7 Tev using 4.5 fb(-1) of data with the ATLAS detector

Çetin, Serkant Ali (Dogus Author)The inclusive jet cross-section is measured in proton-proton collisions at a centre-of-mass energy of 7 TeV using a data set corresponding to an integrated luminosity of 4.5 fb(-1) collected with the ATLAS detector at the Large Hadron Collider in 2011. Jets are identified using the anti-k(t) algorithm with radius parameter values of 0.4 and 0.6. The double-differential cross-sections are presented as a function of the jet transverse momentum and the jet rapidity, covering jet transverse momenta from 100 GeV to 2 TeV. Next-to-leading-order QCD calculations corrected for non-perturbative effects and electroweak effects, as well as Monte Carlo simulations with next-to-leading-order matrix elements interfaced to parton showering, are compared to the measured cross-sections. A quantitative comparison of the measured cross-sections to the QCD calculations using several sets of parton distribution functions is performed
Numerical simulation of wave impact on a rigid wall using a two-phase compressible SPH method

18 pages, 11 figures, 33 references. Other author's papers can be downloaded at http://www.denys-dutykh.com/In this paper, an SPH method based on the SPH--ALE formulation is used for modelling two-phase flows with large density ratios and realistic sound speeds. The SPH scheme is further improved to circumvent the tensile instability that may occur in the SPH simulations. The two-phase SPH solver is then used to model a benchmark problem of liquid impact on a rigid wall. The results are compared with an incompressible Level Set solver. Furthermore, a wave impact on a rigid wall with a large entrained air pocket is modelled. The SPH simulation is initialised by the output of a fully non-linear potential flow solver. The pressure distribution, velocity field and impact pressure are then analysed
High-order Hamiltonian splitting for Vlasov-Poisson equations

International audienceWe consider the Vlasov-Poisson equation in a Hamiltonian framework and derive new time splitting methods based on the decomposition of the Hamiltonian functional between the kinetic and electric energy. Assuming smoothness of the solutions, we study the order conditions of such methods. It appears that these conditions are of  Runge-Kutta-Nyström type. In the one dimensional case, the order conditions can be further simplified, and efficient methods of order 6 with a reduced number of stages can be constructed. In the general case, high-order methods can also be constructed using explicit computations of commutators. Numerical results are performed and show the benefit of using high-order splitting schemes in that context. Complete and self-contained proofs of convergence results and rigorous error estimates are also given
Hippocampal subfield volumetry in mild cognitive impairment, Alzheimer's disease and semantic dementia.

International audienceBACKGROUND: Hippocampal atrophy is a well-known feature of Alzheimer's disease (AD), but sensitivity and specificity of hippocampal volumetry are limited. Neuropathological studies have shown that hippocampal subfields are differentially vulnerable to AD; hippocampal subfield volumetry may thus prove to be more accurate than global hippocampal volumetry to detect AD. METHODS: CA1, subiculum and other subfields were manually delineated from 40 healthy controls, 18 AD, 17 amnestic Mild Cognitive Impairment (aMCI), and 8 semantic dementia (SD) patients using a previously developed high resolution MRI procedure. Non-parametric group comparisons and receiver operating characteristic (ROC) analyses were conducted. Complementary analyses were conducted to evaluate differences of hemispheric asymmetry and anterior-predominance between AD and SD patients and to distinguish aMCI patients with or without β-amyloid deposition as assessed by Florbetapir-TEP. RESULTS: Global hippocampi were atrophied in all three patient groups and volume decreases were maximal in the CA1 subfield (22% loss in aMCI, 27% in both AD and SD; all p < 0.001). In aMCI, CA1 volumetry was more accurate than global hippocampal measurement to distinguish patients from controls (areas under the ROC curve = 0.88 and 0.76, respectively; p = 0.05) and preliminary analyses suggest that it was independent from the presence of β-amyloid deposition. In patients with SD, whereas the degree of CA1 and subiculum atrophy was similar to that found in AD patients, hemispheric and anterior-posterior asymmetry were significantly more marked than in AD with greater involvement of the left and anterior hippocampal subfields. CONCLUSIONS: The findings suggest that CA1 measurement is more sensitive than global hippocampal volumetry to detect structural changes at the pre-dementia stage, although the predominance of CA1 atrophy does not appear to be specific to AD pathophysiological processes
Suppression of Υ(1S)at forward rapidity in Pb–Pb collisions at √sNN=2.76TeV

International audienceWe report on the measurement of the inclusive Υ (1S) production in Pb–Pb collisions at √ s NN = 2.76 TeV carried out at forward rapidity (2.5 < y < 4) and down to zero transverse momentum using its μ + μ − decay channel with the ALICE detector at the Large Hadron Collider. A strong suppression of the inclusive Υ (1S) yield is observed with respect to pp collisions scaled by the number of independent nucleon– nucleon collisions. The nuclear modification factor, for events in the 0–90% centrality range, amounts to 0.30 ± 0.05(stat) ± 0.04(syst). The observed Υ (1S) suppression tends to increase with the centrality of the collision and seems more pronounced than in corresponding mid-rapidity measurements. Our results are compared with model calculations, which are found to underestimate the measured suppression and fail to reproduce its rapidity dependence. (http://creativecommons.org/licenses/by/3.0/). Funded by SCOAP 3
The Herschel Gould Belt Survey in          Chamaeleon II

International audienceContext. We report on the Herschel Gould Belt survey          (HGBS) of the Chamaeleon II (Cha II) star-forming region, focusing on the detection of          Class I to III young stellar objects (YSOs).Aims. We aim at characterizing the circumstellar material around these          YSOs and at understanding which disk parameters are most likely constrained by the new          HGBS data, which are expected to be crucial for studying the transition from optically          thick disks to evolved debris-type disks.Methods. We recovered 29 of the 63 known YSOs in Cha II with a detection          in at least one of the PACS/SPIRE pass-bands: 3 Class I YSOs (i.e.,100%), 1 flat source          (i.e., 50%), 21 Class II objects (i.e., 55%), 3 Class III objects (i.e, 16%), and the          unclassified far-infrared source IRAS 12522-7640. We explored PACS/SPIRE colors of this          sample and modeled their spectral energy distributions (SEDs) from the optical to            Herschel’s wavelengths with the RADMC-2D radiative transfer code.Results. We find that YSO colors are typically confined to the following          ranges:            −0.7 ≲ log (F70/F160) ≲ 0.5,            −0.5 ≲ log (F160/F250) ≲ 0.6,            0.05 ≲ log (F250/F350) ≲ 0.25          and            −0.1 ≲ log (F350/F500) ≲ 0.5.          These color ranges are expected to be only marginally contaminated by extragalactic          sources and field stars and, hence, provide a useful YSO selection tool when applied          together. We were able to model the SED of 26 of the 29 detected YSOs. We discuss the          degeneracy/limitations of our SED fitting results and adopted the Bayesian method to          estimate the probability of different values for the derived disk parameters. The Cha II          YSOs present typical disk inner radii  ≲0.1 AU, as previously estimated in the literature          on the basis of Spitzer data. Our probability analysis shows that, thanks          to the new Herschel data, the lower limits to the disk mass            (Mdisk) and characteristic radius            (RC) are well constrained, while the flaring            angle (1 + φ) is only marginally constrained. The lower limit            to RC is typically around 50 AU. The lower limits to            Mdisk are proportional to the stellar masses with a          typical 0.3% ratio, i.e., in the range estimated in the literature for young Class II          stars and brown dwarfs across a broad range of stellar masses. The estimated flaring          angles, although very uncertain, point toward very flat disks          (1 + φ ≲ 1.2), as found for low-mass M-type YSO samples in other          star-forming regions. Thus, our results support the idea that disk properties show a          dependence on stellar properties
Automated flow cytometric analysis across large numbers of samples and cell types.

International audienceMulti-parametric flow cytometry is a key technology for characterization of immune cell phenotypes. However, robust high-dimensional post-analytic strategies for automated data analysis in large numbers of donors are still lacking. Here, we report a computational pipeline, called FlowGM, which minimizes operator input, is insensitive to compensation settings, and can be adapted to different analytic panels. A Gaussian Mixture Model (GMM)-based approach was utilized for initial clustering, with the number of clusters determined using Bayesian Information Criterion. Meta-clustering in a reference donor permitted automated identification of 24 cell types across four panels. Cluster labels were integrated into FCS files, thus permitting comparisons to manual gating. Cell numbers and coefficient of variation (CV) were similar between FlowGM and conventional gating for lymphocyte populations, but notably FlowGM provided improved discrimination of "hard-to-gate" monocyte and dendritic cell (DC) subsets. FlowGM thus provides rapid high-dimensional analysis of cell phenotypes and is amenable to cohort studies
The effects of an insertion in the 5 ' UTR of the AMCase on gene expression and pulmonary functions

Cataloged from PDF version of article.Background: Studies regarding the physiological role of acidic mammalian chitinase (AMCase) and the effects of its genetic variants on asthma have produced conflicting results. 

Objectives: We aimed to determine the genetic variants in the AMCase gene that could regulate the gene expression and thus influence disease severity. 

Methods: Genetic variants of the AMCase gene were determined by sequencing of asthmatics and healthy controls in up to -1 kb in the promoter region and exon 1 and 2. In an association study, a population of asthmatic (n = 504) and healthy Turkish children (n = 188) were genotyped for the observed SNPs. A replication study was performed in a North American adult population of patients with mild (n = 317) and severe (n = 145) asthma. The functional properties of the insertion were determined by promoter reporter assay, electromobility shift assay and transcription factor ELISA experiments. 

Results: Of the identified SNPs, only a ten base pair insertion (CAATCTAGGC) in the 5'UTR region of exon 2 was significantly associated with lower FEV(1) (beta = -14.63 SE = 6.241, P = 0.019) in Turkish children with asthma. However, in the adult population, the same insertion showed a trend toward higher FEV(1). The insertion was shown to have enhancer activity and the mutant probe possessing the insertion had higher binding affinity for the nuclear extracts. 

Conclusion: Our study shows that a ten base pair insertion in the 5'UTR region of AMCase gene may modify gene expression and thus may affect the severity of asthma. However, its effects appear to be different in different populations. (C) 2011 Elsevier Ltd. All rights reserved
Bub1 maintains centromeric cohesion by activation of the spindle checkpoint.

Bub1 is a component of the spindle assembly checkpoint (SAC), a surveillance mechanism that ensures genome stability by delaying anaphase until all the chromosomes are stably attached to spindle microtubules via their kinetochores. To define Bub1's role in chromosome segregation, embryogenesis, and tissue homeostasis, we generated a mouse strain in which BUB1 can be inactivated by administration of tamoxifen, thereby bypassing the preimplantation lethality associated with the Bub1 null phenotype. We show that Bub1 is essential for postimplantation embryogenesis and proliferation of primary embryonic fibroblasts. Bub1 inactivation in adult males inhibits proliferation in seminiferous tubules, reducing sperm production and causing infertility. In culture, Bub1-deficient fibroblasts fail to align their chromosomes or sustain SAC function, yielding a highly aberrant mitosis that prevents further cell divisions. Centromeres in Bub1-deficient cells also separate prematurely; however, we show that this is a consequence of SAC dysfunction rather than a direct role for Bub1 in protecting centromeric cohesion
Developing pervasive multi-agent systems with nature-inspired coordination

Pervasive computing systems can be modelled effectively as populations of interacting autonomous components. The key challenge to realizing such models is in getting separately-specified and -developed sub-systems to discover and interoperate with each other in an open and extensible way, supported by appropriate middleware services. In this paper, we argue that nature-inspired coordination models offer a promising way of addressing this challenge. We first frame the various dimensions along which nature-inspired coordination models can be defined, and survey the most relevant proposals in the area. We describe the nature-inspired coordination model developed within the SAPERE project as a synthesis of existing approaches, and show how it can effectively support the multifold requirements of modern and emerging pervasive services. We conclude by identifying what we think are the open research challenges in this area, and identify some research directions that we believe are promising
Paramedics' experiences of financial medicine practices in the pre-hospital environment : a pilot study

Abstract: The term “financial medicine” refers to the delivery of health-related services where the generation of financial gain or “profit” takes precedence over the provision of care that is reflective of evidence-based best practice. The practicing of financial medicine includes over-servicing and overbilling, both of which have led to a sharp rise in the cost of health care and medical insurance in South Africa. For this reason, the practicing of financial medicine has been widely condemned both internationally and locally by the Health Professions Council of South Africa (HPCSA) and allied Professional bodies. Objectives: This qualitative pilot study explored and described the experiences of South African Paramedics with regard to the practicing of financial medicine in the local pre- hospital emergency care environment
Magnetic nanoparticles potential for stem cell functionalization

Publicado em "Journal of Tissue Engineering and Regenerative Medicine", vol. 7, supp. 1 (2013)Stem cells have the capacity to migrate within tissues to damaged areas. The ability to remotely monitor and manipulate cells encouraging their precise positioning to desired sites for tissue regeneration would have a potential impact in tissue engineering and regenerative medicine. Cell monitoring and cell localization can be potentially achieved by the internalization of magnetic nanoparticles (MNPs) in the cells. This might allow for the investigation of migratory patterns through tracking studies, the targeting of particle-labeled cells to desired locations via the application of an external magnetic field and, finally, for activation stem cells to initiate various cellular responses to induce the differentiation [1]. The application of a magnetic field can then enhance biological performance through the stimulation of cell proliferation, migration and differentiation.

This study focus on determining the effect of magnetic stimulation in human adipose stem cells (hASCs) behavior in order to establish the interactions between MNPs uptake by the cell, the MNPs concentrations, and magnitude/frequency of the external magnetic field during the internalization process
Measurement of the tt¯ production cross-section as a function of jet multiplicity and jet transverse momentum in 7 TeV proton--proton collisions with the ATLAS detector

The tt¯ production cross-section dependence on jet multiplicity and jet transverse momentum is reported for proton--proton collisions at a centre-of-mass energy of 7 TeV in the single-lepton channel. The data were collected with the ATLAS detector at the CERN Large Hadron Collider and comprise the full 2011 data sample corresponding to an integrated luminosity of 4.6 fb−1. Differential cross-sections are presented as a function of the jet multiplicity for up to eight jets using jet transverse momentum thresholds of 25, 40, 60, and 80 GeV, and as a function of jet transverse momentum up to the fifth jet. The results are shown after background subtraction and corrections for all detector effects, within a kinematic range closely matched to the experimental acceptance. Several QCD-based Monte Carlo models are compared with the results. Sensitivity to the parton shower modelling is found at the higher jet multiplicities, at high transverse momentum of the leading jet and in the transverse momentum spectrum of the fifth leading jet. The MC@NLO+HERWIG MC is found to predict too few events at higher jet multiplicities
Search for the Standard Model Higgs boson in the decay channel H->ZZ(*)->4l with the ATLAS detector

A search for the Standard Model Higgs boson in the decay channel H -> ZZ(()*()) l(+)l(-)l'l'(-), where l = e, mu, is presented. Proton-proton collision data at root s = 7 TeV recorded with the ATLAS detector and corresponding to an average integrated luminosity of 2.1 fb(-1) are compared to the Standard Model expectations. Upper limits on the production cross section of a Standard Model Higgs boson with a mass between 110 and 600 GeV are derived. The observed (expected) 95% confidence level upper limit on the production cross section for a Higgs boson with a mass of 194 GeV, the region with the best expected sensitivity for this search, is 0.99 (1.01) times the Standard Model prediction. The Standard Model Higgs boson is excluded at 95% confidence level in the mass ranges 191-197, 199-200 and 214-224 GeV
Microwave assisted synthesis of dihydrobenzo4,5imidazo1,2-apyrimidin-4- ones; Synthesis, in vitro antimicrobial and anticancer activities of novel coumarin substituted dihydrobenzo4,5imidazo1,2-apyrimidin-4-ones

The present article describes the synthesis of dihydrobenzo4,5imidazo1, 2-apyrimidin-4-one (2a-h) under microwave irradiation. The product was obtained in excellent yield (74-94%) in a shorter reaction time (2 min). These molecules (2a, b) further reacted with various substituted 4-bromomethylcoumarins (3a-f) to yield a new series of coumarin substituted dihydrobenzo4,5imidazo1,2-a pyrimidin-4-ones (4a-h). The structure of all the synthesized compounds were confirmed by spectral studies and screened for their in vitro antibacterial activity against three Gram-positive bacteria viz., Staphylococcus aureus, Enterococcus faecalis, Streptococcus mutans and three Gram-negative bacteria viz., Escherichia coli, Klebsiella pneumonia, Pseudomonas aeruginosa and antifungal activity against Candida albicans, Aspergillus niger, Aspergillus fumigatus, Aspergillus flavus, Fusarium oxysporum, Penicilliumchrysogenum and anticancer activity against Dalton's Ascitic Lymphoma (DAL) cell line. In general, all the compounds possessed better antifungal properties than antibacterial properties. The coumarin substituted dihydrobenzo4,5imidazo1,2- apyrimidin-4-one (4g) (R = i-Pr, R1 = 6-Cl) was found to be the most potent cytotoxic compound (88%) against Dalton's Ascitic Lymphoma cell line at the concentration of 100 Î¼g/mL. Â© 2013 Elsevier Masson SAS. All rights reserved