pinecone/core-2020-05-10-deduplication huggingface dataset:
Regnase-1 and Roquin Regulate a Common Element in Inflammatory mRNAs by Spatiotemporally Distinct Mechanisms

SummaryRegnase-1 and Roquin are RNA binding proteins essential for degradation of inflammation-related mRNAs and maintenance of immune homeostasis. However, their mechanistic relationship has yet to be clarified. Here, we show that, although Regnase-1 and Roquin regulate an overlapping set of mRNAs via a common stem-loop structure, they function in distinct subcellular locations: ribosome/endoplasmic reticulum and processing-body/stress granules, respectively. Moreover, Regnase-1 specifically cleaves and degrades translationally active mRNAs and requires the helicase activity of UPF1, similar to the decay mechanisms of nonsense mRNAs. In contrast, Roquin controls translationally inactive mRNAs, independent of UPF1. Defects in both Regnase-1 and Roquin lead to large increases in their target mRNAs, although Regnase-1 tends to control the early phase of inflammation when mRNAs are more actively translated. Our findings reveal that differential regulation of mRNAs by Regnase-1 and Roquin depends on their translation status and enables elaborate control of inflammation
An ancient dental gene set governs development and continuous regeneration of teeth in sharks

AbstractThe evolution of oral teeth is considered a major contributor to the overall success of jawed vertebrates. This is especially apparent in cartilaginous fishes including sharks and rays, which develop elaborate arrays of highly specialized teeth, organized in rows and retain the capacity for life-long regeneration. Perpetual regeneration of oral teeth has been either lost or highly reduced in many other lineages including important developmental model species, so cartilaginous fishes are uniquely suited for deep comparative analyses of tooth development and regeneration. Additionally, sharks and rays can offer crucial insights into the characters of the dentition in the ancestor of all jawed vertebrates. Despite this, tooth development and regeneration in chondrichthyans is poorly understood and remains virtually uncharacterized from a developmental genetic standpoint. Using the emerging chondrichthyan model, the catshark (Scyliorhinus spp.), we characterized the expression of genes homologous to those known to be expressed during stages of early dental competence, tooth initiation, morphogenesis, and regeneration in bony vertebrates. We have found that expression patterns of several genes from Hh, Wnt/β-catenin, Bmp and Fgf signalling pathways indicate deep conservation over ~450 million years of tooth development and regeneration. We describe how these genes participate in the initial emergence of the shark dentition and how they are redeployed during regeneration of successive tooth generations. We suggest that at the dawn of the vertebrate lineage, teeth (i) were most likely continuously regenerative structures, and (ii) utilised a core set of genes from members of key developmental signalling pathways that were instrumental in creating a dental legacy redeployed throughout vertebrate evolution. These data lay the foundation for further experimental investigations utilizing the unique regenerative capacity of chondrichthyan models to answer evolutionary, developmental, and regenerative biological questions that are impossible to explore in classical models
Reconceptualizing ‘effectiveness’ in environmental projects: Can we measure values-related achievements?

AbstractThere have been recent calls for a shift to an evidence-based paradigm in environmental management, grounded in systematic monitoring and evaluation, but achieving this will be complex and difficult. Evaluating the educational components of environmental initiatives presents particular challenges, because these programs often have multiple concurrent goals and may value 'human outcomes', such as value change, which are intangible and difficult to quantify. This paper describes a fresh approach based on co-creating an entirely new values-based assessment framework with expert practitioners worldwide. We first discuss the development of a generic framework of 'Proto-Indicators' (reference criteria constituting prototypes for measurable indicators), and then demonstrate its application within a reforestation project in Mexico where indicators and assessment tools were localized to enhance context-relevance. Rigorously derived using unitary validity, with an emphasis on relevance, practicability and logical consistency from user perspectives, this framework represents a step-wise advance in the evaluation of non-formal EE/ESD programs. This article also highlights three important principles with broader implications for evaluation, valuation and assessment processes within environmental management: namely peer-elicitation, localizability, and an explicit focus on ethical values. We discuss these principles in relation to the development of sustainability indicators at local and global levels, especially in relation to post-2015 Sustainable Development Goals
Kinetic Asymmetry of Subunit Exchange of Homooligomeric Protein as Revealed by Deuteration-Assisted Small-Angle Neutron Scattering

AbstractWe developed a novel, to our knowledge, technique for real-time monitoring of subunit exchange in homooligomeric proteins, using deuteration-assisted small-angle neutron scattering (SANS), and applied it to the tetradecamer of the proteasome α7 subunit. Isotopically normal and deuterated tetradecamers exhibited identical SANS profiles in 81% D2O solution. After mixing these solutions, the isotope sensitive SANS intensity in the low-q region gradually decreased, indicating subunit exchange, whereas the small-angle x-ray scattering profile remained unchanged confirming the structural integrity of the tetradecamer particles during the exchange. Kinetic analysis of zero-angle scattering intensity indicated that 1), only two of the 14 subunits were exchanged in each tetradecamer and 2), the exchange process involves at least two steps. This study underscores the usefulness of deuteration-assisted SANS, which can provide quantitative information not only on the molecular sizes and shapes of homooligomeric proteins, but also on their kinetic properties
Correlation functions of XX0 Heisenberg chain, q-binomial determinants, and random walks

AbstractThe XX0 Heisenberg model on a cyclic chain is considered. The representation of the Bethe wave functions via the Schur functions allows to apply the well-developed theory of the symmetric functions to the calculation of the thermal correlation functions. The determinantal expressions of the form-factors and of the thermal correlation functions are obtained. The q-binomial determinants enable the connection of the form-factors with the generating functions both of boxed plane partitions and of self-avoiding lattice paths. The asymptotical behavior of the thermal correlation functions is studied in the limit of low temperature provided that the characteristic parameters of the system are large enough
Improvement of the NOx selectivity for a planar YSZ sensor

AbstractThe present work aims to develop a sensor that can leave the laboratory and be industrialised. For this reason potentiometric sensors based on a solid electrolyte YSZ and 2 metallic electrodes (platinum and gold) were fabricated by screenprinting technology and were tested in a laboratory test bench for different concentrations of CO, NO and NO2 in oxygen rich atmosphere. By deposing a catalytic filter consisting of 1.7%Pt dispersed in alumina directly on the electrodes, it was shown that the selectivity towards NOx could be highly reinforced. This filter was characterized by the use of SEM, TPD, and XRD technology
Higher-dimensional supersymmetry as an origin of the three families for quarks and leptons

AbstractIn a (0,1) supersymmetric (SUSY) six-dimensional gauge theory, a gauge fermion gives rise to box anomalies. These anomalies are completely canceled by assuming a vector multiplet of (1,1) SUSY. With a T2/Z3 orbifold compactification of the extra two-dimensional space, the theory provides three chiral multiplets and three equivalent fixed points. We regard them as the origin of the three families of quarks and leptons. Quasi anarchy structure in the SU(5)-5∗ sector and hence the bilarge mixing in the neutrino oscillation are explained quite naturally in this framework. We also discuss a family symmetry as a remnant of the higher-dimensional R symmetry
A Financial Engineering Approach to Identify Stock Market Bubble

AbstractIn this paper we adopt an engineering method based on Al-Anaswah and Wilfling, state space model with Markov-switching, to capture the speculative bubbles of stock markets in China and US. We present the VAR log linear asset pricing model in state space model with Markov-switching, so that we can capture the unobservable speculative bubbles. Based on the dataset from Stock markets in China and US, we find empirically that the engineering technique we choose detect the stock markets bubbles effectively, and that the switching probabilities between the surviving and collapsing regimes. In-the-sample and out-of-sample forecasting further support our empirical evidence
Linear response theory for magnetic Schrödinger operators in disordered media

AbstractWe justify the linear response theory for an ergodic Schrödinger operator with magnetic field within the noninteracting particle approximation, and derive a Kubo formula for the electric conductivity tensor. To achieve that, we construct suitable normed spaces of measurable covariant operators where the Liouville equation can be solved uniquely. If the Fermi level falls into a region of localization, we recover the well-known Kubo–Str˘eda formula for the quantum Hall conductivity at zero temperature
Multiple Mechanistically Distinct Modes of Endocannabinoid Mobilization at Central Amygdala Glutamatergic Synapses

SummaryThe central amygdala (CeA) is a key structure at the limbic-motor interface regulating stress responses and emotional learning. Endocannabinoid (eCB) signaling is heavily implicated in the regulation of stress-response physiology and emotional learning processes; however, the role of eCBs in the modulation of synaptic efficacy in the CeA is not well understood. Here we describe the subcellular localization of CB1 cannabinoid receptors and eCB synthetic machinery at glutamatergic synapses in the CeA and find that CeA neurons exhibit multiple mechanistically and temporally distinct modes of postsynaptic eCB mobilization. These data identify a prominent role for eCBs in the modulation of excitatory drive to CeA neurons and provide insight into the mechanisms by which eCB signaling and exogenous cannabinoids could regulate stress responses and emotional learning
Long-range angular correlations of π, K and p in p–Pb collisions at sNN=5.02 TeV

AbstractAngular correlations between unidentified charged trigger particles and various species of charged associated particles (unidentified particles, pions, kaons, protons and antiprotons) are measured by the ALICE detector in p–Pb collisions at a nucleon–nucleon centre-of-mass energy of 5.02 TeV in the transverse-momentum range 0.3<pT<4 GeV/c. The correlations expressed as associated yield per trigger particle are obtained in the pseudorapidity range |ηlab|<0.8. Fourier coefficients are extracted from the long-range correlations projected onto the azimuthal angle difference and studied as a function of pT and in intervals of event multiplicity. In high-multiplicity events, the second-order coefficient for protons, v2p, is observed to be smaller than that for pions, v2π, up to about pT=2 GeV/c. To reduce correlations due to jets, the per-trigger yield measured in low-multiplicity events is subtracted from that in high-multiplicity events. A two-ridge structure is obtained for all particle species. The Fourier decomposition of this structure shows that the second-order coefficients for pions and kaons are similar. The v2p is found to be smaller at low pT and larger at higher pT than v2π, with a crossing occurring at about 2 GeV/c. This is qualitatively similar to the elliptic-flow pattern observed in heavy-ion collisions. A mass ordering effect at low transverse momenta is consistent with expectations from hydrodynamic model calculations assuming a collectively expanding system
Role of interplay between IL-4 and IFN-γ in the in regulating M1 macrophage polarization induced by Nattectin

AbstractRecently our group described that Nattectin, a C-type lectin of the venom of Thalassophryne nattereri shows a potent pro-inflammatory capacity. Here, we demonstrated that Nattectin is able to induce M1 macrophage marker iNOS, and up-regulate the expression of MHC class II, CD80, CD86 and CD40 molecules. The increase in MHC class II and CD49a integrin expression with MMP-9 production and endocytic capacity depend on lectin function of Nattectin. Moreover, the polarization of peritoneal and bone marrow-derived macrophages induced by Nattectin to M1 profile is dependent on Th1 cytokines (IL-12 and IFN-γ), and negatively regulated by Th2 cytokines (IL-4, IL-10 and IL-13). Also we reveal that IL-4 play a dual role in this polarization: a regular action of IL-4 was seen in the negative regulation of the CD40 expression, but an unexpected positive regulation was seen in the expression of CCR7 and MHC class II. Finally, our in vivo studies showed that the influx of neutrophils and small peritoneal macrophage - F4/80lowMHCIIhi induced by Nattectin is totally dependent on IL-4 and IFN-γ cytokines. Furthermore, the induction of IL-6 release is negatively regulated by IL-4 and positively regulated by IL-12 and IFN-γ. Together, the results allowed us to expand the knowledge about the regulation of macrophage activation, as well as confirmed the ability of Nattectin, a fish C-type lectin, as an important immunomodulatory agent
Global decomposition of a Lorentzian manifold as a Generalized Robertson–Walker space

AbstractGeneralized Robertson–Walker (GRW) spaces constitute a quite important family in Lorentzian geometry, and it is an interesting question to know whether a Lorentzian manifold can be decomposed in such a way. It is well known that the existence of a suitable vector field guaranties the local decomposition of the manifold. In this paper, we give conditions on the curvature which ensure a global decomposition and apply them to several situations where local decomposition appears naturally. We also study the uniqueness question, obtaining that the de Sitter spaces are the only nontrivial complete Lorentzian manifolds with more than one GRW decomposition. Moreover, we show that the Friedmann Cosmological Models admit an unique GRW decomposition, even locally
Observation of a peaking structure in the J/ψϕ mass spectrum from B±→J/ψϕK± decays

AbstractA peaking structure in the J/ψϕ mass spectrum near threshold is observed in B±→J/ψϕK± decays, produced in pp collisions at s=7 TeV collected with the CMS detector at the LHC. The data sample, selected on the basis of the dimuon decay mode of the J/ψ, corresponds to an integrated luminosity of 5.2 fb−1. Fitting the structure to an S-wave relativistic Breit–Wigner lineshape above a three-body phase-space nonresonant component gives a signal statistical significance exceeding five standard deviations. The fitted mass and width values are m=4148.0±2.4(stat.)±6.3(syst.)MeV and Γ=28−11+15(stat.)±19(syst.)MeV, respectively. Evidence for an additional peaking structure at higher J/ψϕ mass is also reported
From computer ethics to responsible research and innovation in ICT The transition of reference discourses informing ethics-related research in information systems

AbstractThe discourse concerning computer ethics qualifies as a reference discourse for ethics-related IS research. Theories, topics and approaches of computer ethics are reflected in IS. The paper argues that there is currently a broader development in the area of research governance, which is referred to as ‘responsible research and innovation’ (RRI). RRI applied to information and communication technology (ICT) addresses some of the limitations of computer ethics and points toward a broader approach to the governance of science, technology and innovation. Taking this development into account will help IS increase its relevance and make optimal use of its established strengths
Elucidation of the mechanisms of action of Bacteriophage K/nano-emulsion formulations against S. aureus via measurement of particle size and zeta potential

AbstractIn earlier work we have demonstrated the effect that nano-emulsions have on bacterial growth, and most importantly the enhanced bacteriophage infectivity against Staphylococcus aureus in planktonic culture when phage are carried in nano-emulsions. However, the mechanisms of enhancement of the bacteriophage killing effect are not specifically understood. This work focuses on the investigation of the possible interactions between emulsion droplets and bacterial cells, between emulsion droplets and bacteriophages, and finally interactions between all three components: nano-emulsion droplets, bacteria, and bacteriophages. The first approach consists of simple calculations to determine the spatial distribution of the components, based on measurements of particle size. It was found that nano-emulsion droplets are much more numerous than bacteria or bacteriophage, and due to their size and surface area they must be covering the surface of both cells and bacteriophage particles. Stabilisation of bacteriophages due to electrostatic forces and interaction with nano-emulsion droplets is suspected, since bacteriophages may be protected against inactivation due to ‘charge shielding’. Zeta potential was measured for the individual components in the system, and for all of them combined. It was concluded that the presence of nano-emulsions could be reducing electrostatic repulsion between bacterial cells and bacteriophage, both of which are very negatively ‘charged’. Moreover, nano-emulsions lead to more favourable interaction between bacteriophages and bacteria, enhancing the anti-microbial or killing effect. These findings are relevant since the physicochemical properties of nano-emulsions (i.e. particle size distribution and zeta potential) are key in determining the efficacy of the formulation against infection in the context of responsive burn wound dressings—which is the main target for this work.Data Access: All data created during this research are openly available from the University of Bath data archive at http://doi.org/10.15125/BATH-00166
Estrogen receptor subtype β2 is involved in neuromast development in zebrafish (Danio rerio) larvae

AbstractEstrogens are known to play a role in both reproductive and non-reproductive functions in mammals. Estrogens and their receptors are involved in the development of the central nervous system (brain development, neuronal survival and differentiation) as well as in the development of the peripheral nervous system (sensory-motor behaviors). In order to decipher possible functions of estrogens in early development of the zebrafish sensory system, we investigated the role of estrogen receptor β2 (ERβ2) by using a morpholino (MO) approach blocking erβ2 RNA translation. We further investigated the development of lateral line organs by cell-specific labeling, which revealed a disrupted development of neuromasts in morphants. The supporting cells developed and migrated normally. Sensory hair cells, however, were absent in morphants' neuromasts. Microarray analysis and subsequent in situ hybridizations indicated an aberrant activation of the Notch signaling pathway in ERβ2 morphants. We conclude that signaling via ERβ2 is essential for hair cell development and may involve an interaction with the Notch signaling pathway during cell fate decision in the neuromast maturation process
New Trends in Teacher's Education. Educational Placement of the Adopted Child

AbstractIn Italy, the number of adopted school-age children is increasing. According to the Commission of Intercountry Adoption (2013), 3106 children were adopted, 47.5% of them are between 5 and 9 years old. The present action-research aims at exploring the spread of good approaches in schools in terms of welcoming of adopted children. For this purpose, 268 teachers of primary schools were involved in analyzing the social representation about adopted children and their family. The results show a simplified vision of the adoptive family, which is described as heroic family or, on the contrary, as a family with difficulty
Cellular activation of hypothalamic hypocretin/orexin neurons facilitates short-term spatial memory in mice.

The hypothalamic hypocretin/orexin (HO) system holds a central role in the regulation of several physiological functions critical for food-seeking behavior including mnemonic processes for effective foraging behavior. It is unclear however whether physiological increases in HO neuronal activity can support such processes. Using a designer rM3Ds receptor activation approach increasing HO neuronal activity resulted in improved short-term memory for novel locations. When tested on a non-spatial novelty object recognition task no significant difference was detected between groups indicating that hypothalamic HO neuronal activation can selectively facilitate short-term spatial memory for potentially supporting memory for locations during active exploration.This work was supported by the Royal Society – UK (JAS), Sigrid Juselius Foundation (TA) and Erasmus Scholarship (EP).Open Access funded by Wellcome Trust.Peer-reviewedPublisher Versio
Observation of the decay $B_{s}^{0} \to \eta_{c} \phi$ and evidence for
  $B_{s}^{0} \to \eta_{c} \pi^{+} \pi^{-} $

A study of $B^{0}_{s} \to \eta_{c} \phi$ and $B^{0}_{s} \to \eta_{c}
\pi^{+}\pi^{-}$ decays is performed using $pp$ collision data corresponding to
an integrated luminosity of 3.0$\,\rm fb^{-1}$, collected with the LHCb
detector in Run~1 of the LHC. The observation of the decay $B^{0}_{s} \to
\eta_{c} \phi$ is reported, where the $\eta_{c}$ meson is reconstructed in the
$p\bar p$, $K^+K^-\pi^+\pi^-$, $\pi^+\pi^-\pi^+\pi^-$ and $K^+K^-K^+K^-$ decay
modes and the $\phi(1020)$ in the $K^+ K^-$ decay mode. The decay $B^{0}_{s}
\to J/\psi \phi$ is used as a normalisation channel. Evidence is also reported
for the decay $B^{0}_{s} \to \eta_{c} \pi^{+}\pi^{-}$, where the $\eta_{c}$
meson is reconstructed in the $p\bar p$ decay mode, using the decay $B^{0}_{s}
\to J/\psi \pi^+ \pi^-$ as a normalisation channel. The measured branching
fractions are \begin{eqnarray*} {\mathcal B (B^{0}_{s} \to \eta_{c} \phi)} &=&
\left(5.01 \pm 0.53 \pm 0.27 \pm 0.63 \right) \times 10^{-4} \,, \nonumber
  \\ {\mathcal B (B^{0}_{s} \to \eta_{c} \pi^+ \pi^-)} &=& \left(1.76 \pm 0.59
\pm 0.12 \pm 0.29 \right) \times 10^{-4} \,, \end{eqnarray*} where in each case
the first uncertainty is statistical, the second systematic and the third
uncertainty is due to the limited knowledge of the external branching
fractions.Comment: 30 pages, 9 figures All figures and tables, along with any
  supplementary material and additional information, are available at
  https://lhcbproject.web.cern.ch/lhcbproject/Publications/LHCbProjectPublic/LHCb-PAPER-2016-056.htm