pinecone/core-2020-05-10-deduplication huggingface dataset:
Utilization of cellulose microcapillary tubes as a model system for culturing and viral infection of mammalian cells

Cryofixation by high-pressure freezing (HPF) and freeze substitution (FS) gives
excellent preservation of intracellular membranous structures, ideal for ultrastructural
investigations of virus infected cells. Conventional sample preparation methods of tissue
cultured cells can however disrupt the association between neighbouring cells or of
viruses with the plasma membrane, which impacts upon the effectiveness whereby
virus release from cells can be studied. We established a system for virus infection and
transmission electron microscopy preparation of mammalian cells that allowed optimal
visualisation of membrane release events. African horse sickness virus (AHSV) is a
non-enveloped virus that employs two different release mechanisms from mammalian cells, i.e. lytic release through a disrupted plasma membrane and a non-lytic buddingtype
release. Cellulose microcapillary tubes were used as support layer for culturing
Vero cells. The cells grew to a confluent monolayer along the inside of the tubes and
could readily be infected with AHSV. Sections of the microcapillary tubes proved easy
to manipulate during the HPF procedure, showed no distortion or compression, and
yielded well preserved cells in their native state. There was ample cell surface area
available for visualisation, which allowed detection of both types of virus release at the
plasma membrane at a significantly higher frequency than when utilising other methods.
The consecutive culturing, virus infection and processing of cells within microcapillary
tubes therefore represent a novel model system for monitoring intracellular virus life
cycle and membrane release events, specifically suited to viruses that do not grow to
high titres in tissue culture.http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1097-002
Inactivation of SACE_3446, a TetR family transcriptional regulator, stimulates erythromycin production in Saccharopolyspora erythraea

AbstractErythromycin A is a widely used antibiotic produced by Saccharopolyspora erythraea; however, its biosynthetic cluster lacks a regulatory gene, limiting the yield enhancement via regulation engineering of S. erythraea. Herein, six TetR family transcriptional regulators (TFRs) belonging to three genomic context types were individually inactivated in S. erythraea A226, and one of them, SACE_3446, was proved to play a negative role in regulating erythromycin biosynthesis. EMSA and qRT-PCR analysis revealed that SACE_3446 covering intact N-terminal DNA binding domain specifically bound to the promoter regions of erythromycin biosynthetic gene eryAI, the resistant gene ermE and the adjacent gene SACE_3447 (encoding a long-chain fatty-acid CoA ligase), and repressed their transcription. Furthermore, we explored the interaction relationships of SACE_3446 and previously identified TFRs (SACE_3986 and SACE_7301) associated with erythromycin production. Given demonstrated relatively independent regulation mode of SACE_3446 and SACE_3986 in erythromycin biosynthesis, we individually and concomitantly inactivated them in an industrial S. erythraea WB. Compared with WB, the WBΔ3446 and WBΔ3446Δ3986 mutants respectively displayed 36% and 65% yield enhancement of erythromycin A, following significantly elevated transcription of eryAI and ermE. When cultured in a 5 L fermentor, erythromycin A of WBΔ3446 and WBΔ3446Δ3986 successively reached 4095 mg/L and 4670 mg/L with 23% and 41% production improvement relative to WB. The strategy reported here will be useful to improve antibiotics production in other industrial actinomycete
The state of the art on European well-being research within the area of mental health

AbstractAs part of A Roadmap for Mental Health Research in Europe project, the aim of the present study was to perform a systematic mapping of the main publications in peer-reviewed journals for well-being research within the area of mental health or mental disorders in Europe. The PubMed and PsycINFO databases were used to identify papers on well-being within the area of mental health and mental disorders published from January 2007 to September 2014. Mean 5-year impact factors were obtained. The number of publications for each country was analysed by population size and gross domestic product (GDP). A total of 4,423 unique publications were identified. The number of publications increased for the analysed time period. France and the Netherlands had the highest 5-year mean impact factor. Publications per capita were higher in the Nordic countries, Ireland and the Netherlands. After adjusting for GPD, the most productive countries were the Nordic countries and the Netherlands. There is a marked variation in well-being publications by country in Europe. Eastern European countries produce little research taking into consideration the levels of resources available. Research on older adults was underrepresented and should be prioritised
Super-exponential inflation from a dynamical foliation of a 5D vacuum state

AbstractWe introduce super-exponential inflation (ω<−1) from a 5D Riemann-flat canonical metric on which we make a dynamical foliation. The resulting metric describes a super accelerated expansion for the early universe well known as super-exponential inflation that, for very large times, tends to an asymptotic de Sitter (vacuum dominated) expansion. The scalar field fluctuations are analyzed. The important result here obtained is that the spectral index for energy density fluctuations is not scale invariant, and for cosmological scales becomes ns(k<k⁎)≃1. However, for astrophysical scales this spectrum changes to negative values ns(k>k⁎)<0
Domain-Domain Interactions in Filamin A (16–23) Impose a Hierarchy of Unfolding Forces

AbstractThe quaternary structure of Filamin A (FLNa) 16–23 was recently shown to exhibit multiple domain-domain interactions that lead to a propeller-like construction. Here we present single-molecule force spectroscopy experiments to show a wide variety of mechanical responses of this molecule and compare it with its linear counterpart FLNa 1–8. The compact structure of FLNa 16–23 leads to a broad distribution of rupture forces and end-to-end lengths in the force-extension mode and multiple unraveling timescales in the force-clamp mode. Moreover, a subset of force-extension trajectories reveals a mechanical hierarchy in which the rupture of domain-domain interactions at high forces (>200 pN) liberates the unfolding of individual domains at low forces (∼100 pN). This mechanism may also explain the order-of-magnitude difference in the rates of the biexponential fits to the distribution of unfolding dwell times under force-clamp. Overall, FLNa 16–23 under a force of 100 pN is more compliant than the linear FLNa 1–8. Because a physiological role of FLNa is to crosslink actin filaments, this range of responses allows it to accommodate a broad spectrum of forces exerted by the cell and its environment
Perturbative QCD study of Bs decays to a pseudoscalar meson and a tensor meson

AbstractWe study two-body hadronic Bs→PT decays, with P(T) being a light pseudoscalar (tensor) meson, in the perturbative QCD approach. The CP-averaged branching ratios and the direct CP asymmetries of the ΔS=0 modes are predicted, where ΔS is the difference between the strange numbers of final and initial states. We also define and calculate experimental observables for the ΔS=1 modes under the Bs0–B¯s0 mixing, including CP averaged branching ratios, time-integrated CP asymmetries, and the CP observables Cf, Df and Sf. Results are compared to the Bs→PV ones in the literature, and to the B→PT ones, which indicate considerable U-spin symmetry breaking. Our work provides theoretical predictions for the Bs→PT decays for the first time, some of which will be potentially measurable at future experiments
Production activities and economic dependency by age and gender in Europe: A cross-country comparison

AbstractWe compare selected European countries using an economic dependency ratio which emphasizes the role of age-specific levels of production and consumption. Our analysis reveals large differences in the age- and gender-specific level and type of production activities across selected European countries and identifies possible strategies to adjust age-specific economic behaviour to an ageing population. The cross-country differences in economic dependency of children and elderly persons are largely determined by the age at which people enter, respectively exit, the labour market. The ability of the working age population to support children and elderly persons in turn is strongly influenced by the participation of women in paid work. We also provide a measure for the age-specific production and consumption in form of unpaid household work. The inclusion of unpaid household work leads to a decrease of the gender differences in production activities and indicates that the working age population supports children and elderly persons not only through monetary transfers but also through services produced by unpaid work (e.g. childcare, cooking, cleaning…). Given the available data, we cannot distinguish the age profile of consumption by gender and have to assume – in case of unpaid work - that each member of the household consumes the same. Hence, our results have to be regarded as a first approximation only. Our paper aims to argue that a reform of the welfare system needs to take into account not only public transfers but also private transfers, in particular the transfers in form of goods and services produced through unpaid household work
Housing and the (re)configuration of energy provision in Cape Town and São Paulo: Making space for a progressive urban climate politics?

AbstractThis paper takes as its starting point the argument that infrastructure networks cannot merely be thought of as the backdrop against which climate politics is played out in the city, but are instead fundamental to the ways in which this is conducted. Bringing infrastructure networks into view as a central tenet to the governing of climate change is not simply a matter of recognizing such spheres as critical sites for urban climate policy, but rather requires a conceptual approach that moves beyond a concern with the capacities of individual actors and institutions, and engages with the ways in which power operates and is constituted through the socio-materiality of the city. Drawing on theories of governmentality we develop such an approach and use it to examine the ways in which housing is providing a means through which experimentation with climate governance is taking place in Cape Town and São Paulo. Through this analysis we find that climate change is being mobilized both as a means to create new flows of carbon finance and as a means through which to advance calls for social inclusion and ‘dignified’ housing. Rather than being characterized by one dominant model, we suggest that the governing of climate change in cities in the global South is multiple, combining market and social logics in eclectic ways, where one is not predominant and both are in constant negotiation, dialogue and contestation
Quantum spin models for the SU(n)1 Wess–Zumino–Witten model

AbstractWe propose 1D and 2D lattice wave functions constructed from the SU(n)1 Wess–Zumino–Witten (WZW) model and derive their parent Hamiltonians. When all spins in the lattice transform under SU(n) fundamental representations, we obtain a two-body Hamiltonian in 1D, including the SU(n) Haldane–Shastry model as a special case. In 2D, we show that the wave function converges to a class of Halperin's multilayer fractional quantum Hall states and belongs to chiral spin liquids. Our result reveals a hidden SU(n) symmetry for this class of Halperin states. When the spins sit on bipartite lattices with alternating fundamental and conjugate representations, we provide numerical evidence that the state in 1D exhibits quantum criticality deviating from the expected behaviors of the SU(n)1 WZW model, while in 2D they are chiral spin liquids being consistent with the prediction of the SU(n)1 WZW model
Reduction of anionic sites in the glomerular basement membrane by heparanase does not lead to proteinuria

Heparan sulfate in the glomerular basement membrane has been considered crucial for charge-selective filtration. In many proteinuric diseases, increased glomerular expression of heparanase is associated with decreased heparan sulfate. Here, we used mice overexpressing heparanase and evaluated the expression of different heparan sulfate domains in the kidney and other tissues measured with anti-heparan sulfate antibodies. Glycosaminoglycan-associated anionic sites were visualized by the cationic dye cupromeronic blue. Transgenic mice showed a differential loss of heparan sulfate domains in several tissues. An unmodified and a sulfated heparan sulfate domain resisted heparanase action in vivo and in vitro. Glycosaminoglycan-associated anionic sites were reduced about fivefold in the glomerular basement membrane of transgenic mice, whereas glomerular ultrastructure and renal function remained normal. Heparanase-resistant heparan sulfate domains may represent remnant chains or chains not susceptible to cleavage. Importantly, the strong reduction of glycosaminoglycan-associated anionic sites in the glomerular basement membrane without development of a clear renal phenotype questions the primary role of heparan sulfate in charge-selective filtration. We cannot, however, exclude that overexpression of heparanase and heparan sulfate loss in the basement membrane in glomerular diseases contributes to proteinuria
Selective impairment of global motion integration, but not global form detection, in schizophrenia and bipolar affective disorder

AbstractRecent evidence suggests that schizophrenia is associated with impaired processing of global visual motion, but intact processing of global visual form. This project assessed whether preserved visual form detection in schizophrenia extended beyond low-level pattern discrimination to a naturalistic form-detection task. We assessed both naturalistic form detection and global motion detection in individuals with schizophrenia spectrum disorder, bipolar affective disorder, and healthy controls. Individuals with schizophrenia spectrum disorder and bipolar affective disorder were impaired relative to healthy controls on the global motion task, but not the naturalistic form-detection task. Results indicate that preservation of visual form detection in these disorders extends beyond configural forms to naturalistic object processing
The two-boundary Temperley–Lieb algebra

AbstractWe study a two-boundary extension of the Temperley–Lieb algebra which has recently arisen in statistical mechanics. This algebra lies in a quotient of the affine Hecke algebra of type C and has a natural diagrammatic representation. The algebra has three parameters and, for generic values of these, we determine its representation theory.We use the action of the centre of the affine Hecke algebra to show that all irreducible representations lie within a finite dimensional diagrammatic quotient. These representations are fully characterised by an additional parameter related to the action of the centre. For generic values of this parameter there is a unique representation of dimension 2N and we show that it is isomorphic to a tensor space representation. We construct a basis in which the Gram matrix is diagonal and use this to discuss the irreducibility of this representation
Search for new phenomena in final states with large jet multiplicities and missing transverse momentum with ATLAS using s=13 TeV proton–proton collisions

AbstractResults are reported of a search for new phenomena, such as supersymmetric particle production, that could be observed in high-energy proton–proton collisions. Events with large numbers of jets, together with missing transverse momentum from unobserved particles, are selected. The data analysed were recorded by the ATLAS experiment during 2015 using the 13 TeV centre-of-mass proton–proton collisions at the Large Hadron Collider, and correspond to an integrated luminosity of 3.2 fb−1. The search selected events with various jet multiplicities from ≥7 to ≥10 jets, and with various b-jet multiplicity requirements to enhance sensitivity. No excess above Standard Model expectations is observed. The results are interpreted within two supersymmetry models, where gluino masses up to 1400 GeV are excluded at 95% confidence level, significantly extending previous limits
Triage of Patients With Moderate to Severe Heart Failure Who Should Be Referred to a Heart Failure Center?

ObjectivesThe purpose of this study was to evaluate simple criteria for referral of patients from the general practitioner to a heart failure (HF) center.BackgroundIn advanced HF, the criteria for heart transplantation, left ventricular assist device, and palliative care are well known among HF specialists, but criteria for referral to an advanced HF center have not been developed for generalists.MethodsWe assessed observed and expected all-cause mortality in 10,062 patients with New York Heart Association (NYHA) functional class III to IV HF and ejection fraction <40% registered in the Swedish Heart Failure Registry between 2000 and 2013. Next, 5 pre-specified universally available risk factors were assessed as potential triggers for referral, using multivariable Cox regression: systolic blood pressure ≤90 mm Hg; creatinine ≥160 μmol/l; hemoglobin ≤120 g/l; no renin-angiotensin system antagonist; and no beta-blocker.ResultsIn NYHA functional class III to IV and age groups ≤65 years, 66 to 80 years, and >80 years, there were 2,247, 4,632, and 3,183 patients, with 1-year observed versus expected survivals of 90% versus 99%, 79% versus 97%, and 61% versus 89%, respectively. In the age ≤80 years group, the presence of 1, 2, or 3 to 5 of these risk factors conferred an independent hazard ratio for all-cause mortality of 1.40, 2.30, and 4.07, and a 1-year survival of 79%, 60%, and 39%, respectively (p < 0.001).ConclusionsIn patients ≤80 years of age with NYHA functional class III to IV HF and ejection fraction <40%, mortality is predominantly related to HF or its comorbidities. Potential heart transplantation/left ventricular assist device candidacy is suggested by ≥1 risk factor and potential palliative care by multiple universally available risk factors. These patients may benefit from referral to an advanced HF center
Rational quantum integrable systems of DN type with polarized spin reversal operators

AbstractWe study the spin Calogero model of DN type with polarized spin reversal operators, as well as its associated spin chain of Haldane–Shastry type, both in the antiferromagnetic and ferromagnetic cases. We compute the spectrum and the partition function of the former model in closed form, from which we derive an exact formula for the chain's partition function in terms of products of partition functions of Polychronakos–Frahm spin chains of type A. Using a recursion relation for the latter partition functions that we derive in the paper, we are able to numerically evaluate the partition function, and thus the spectrum, of the DN-type spin chain for relatively high values of the number of spins N. We analyze several global properties of the chain's spectrum, such as the asymptotic level density, the distribution of consecutive spacings of the unfolded spectrum, and the average degeneracy. In particular, our results suggest that this chain is invariant under a suitable Yangian group, and that its spectrum coincides with that of a Yangian-invariant vertex model with linear energy function and dispersion relation
Neutrino masses induced by R-parity violation in a SUSY SU(5) model with additional 5̄′L+5′L

AbstractWithin the framework of an SU(5) SUSY GUT model, a possible general form of the neutrino mass matrix induced by R-parity violation is investigated. The model has matter fields 5̄′L+5′L in addition to the ordinary matter fields 5̄L+10L and Higgs fields Hu+H̄d. The R-parity violating terms are given by 5̄L5̄L10L, while the Yukawa interactions are given by H̄d5̄′L10L. Since the matter fields 5̄′L and 5̄L are different from each other at the unification scale, the R-parity violation effects at a low energy scale appear only through the 5̄′L↔5̄L mixings. In order to make this R-parity violation effect harmless for proton decay, a discrete symmetry Z3 and a triplet–doublet splitting mechanism analogous to that in the 5-plet Higgs fields are assumed
Integer point sets minimizing average pairwise L1 distance: What is the optimal shape of a town?

AbstractAn n-town, n∈N, is a group of n buildings, each occupying a distinct position on a 2-dimensional integer grid. If we measure the distance between two buildings along the axis-parallel street grid, then an n-town has optimal shape if the sum of all pairwise Manhattan distances is minimized. This problem has been studied for cities, i.e., the limiting case of very large n. For cities, it is known that the optimal shape can be described by a differential equation, for which no closed-form solution is known. We show that optimal n-towns can be computed in O(n7.5) time. This is also practically useful, as it allows us to compute optimal solutions up to n=80
X-Klaim and Klava Programming Mobile Code

AbstractHighly distributed networks have now become a common infrastructure for a new kind of wide-area distributed applications whose key design principle is network awareness, namely the ability to deal with dynamic changes of the network environment. Network-aware computing has called for new programming languages that exploit the mobility paradigm as the basic interaction mechanism. In this paper we present the Klaim (Kernel Language for Agent Interaction and Mobility) framework for programming mobile code applications, namely the X Klaim programming language and the Java-based run-time system Klava. In particular, we illustrate how Klava handles mobile code. Finally, an example is shown that is implemented using this framework
Leptonic CP phases near the μ–τ symmetric limit

AbstractThe neutrino masses and mixings indicated by current neutrino oscillation experiments suggest that the neutrino mass matrix possesses an approximate μ–τ exchange symmetry. In this study, we explore the neutrino parameter space and show that if a small μ–τ symmetry breaking is considered, the Majorana CP phases must be unequal and non-zero independently of the neutrino mass scale. Moreover, a small μ–τ symmetry breaking favors quasi-degenerate masses. We also show that Majorana phases are strongly correlated with the Dirac CP violating phase. Within this framework, we obtain robust predictions for the values of the Majorana phases when the experimental indications for the Dirac CP phase are used