pinecone/core-2020-05-10-deduplication huggingface dataset:
Fine-needle aspiration cytology in the follow-up of Hodgkin lymphoma

Hodgkin lymphoma (HL) is characterized by long survival and risk of relapse and second neoplasm. The aim of this study is to evaluate the possibility of improving the accuracy of fine-needle cytology (FNC) in HL follow-up using Power Doppler ultrasound (US) assistance and immediate microscopic evaluation (ICE). The study was performed in two consecutive groups of 200 FNC in HL patients. In the first group FNC of palpable lymphnodes or extra lymph-nodal masses were performed without US assistance except for impalpable and/or deep located masses (nonassisted group); In the second group, all the FNC were performed under Power Doppler US assistance with ICE and immediately repeated in inadequate cases (assisted group). Cytological diagnoses were controlled by histology (61) or clinical follow-up (69); sensitivity and specificity were calculated in the two groups and to evaluate the effect of Power Doppler alone, adequate cases were compared with the total number of FNC in each of the two groups. FNC identified 90 negative cases, 3 false negatives, 70 HL relapse, 16 inadequate and 14 suspicious; second neoplasia were diagnosed in 12 cases and all histologically confirmed. Sensitivity and specificity were 64 and 84% in the nonassisted group and 86 and 94% in the assisted group and there were significant differences between the number of adequate cases v.s. the total number of FNC in each of the two groups. Sensitivity and specificity in assisted FNC are higher than in nonassisted ones. The main advantage of assisted FNC in the follow-up of HL is to produce accurate diagnoses avoiding invasive biopsies
Application of immunocytochemistry and  BRAF  mutational analysis to direct smears of metastatic melanoma

BACKGROUND:  The cytodiagnosis of melanoma in fine‐needle aspiration (FNA) specimens can be challenging, often requiring the use of immunocytochemistry. As constitutively activating mutations in the  BRAF  oncogene are present in at least 40% of melanomas, the use of FNA material to interrogate the  BRAF  mutational status is likely to increase. Because cell blocks, traditionally used for these studies, can occasionally exhibit insufficient tumor cellularity, the authors investigated the utility of direct smears for immunocytochemistry and  BRAF  mutational analysis.    METHODS:  Immunocytochemistry for S‐100, HMB‐45, and Mart‐1 was prospectively performed on direct smears in 17 FNAs of metastatic melanoma. Next,  BRAF  sequencing was performed using DNA isolated from archived Diff‐Quik–stained direct smears for 15 cases. In parallel, sequencing was performed using DNA obtained from corresponding cell blocks.    RESULTS:  S‐100 positivity in the tumor cells was observed in all 17 cases. HMB‐45 and Mart‐1 positivity was noted in 81% and 88% of cases, respectively. All 3 markers were positive in 76% of cases. Next, of the 15 archived melanoma FNAs tested,  BRAF  mutations were observed in 8 (53%); 5 and 3 melanomas harbored the V600E and V600K mutation, respectively. Corresponding cell blocks were also tested for all 15 cases, yielding concordant  BRAF  results in 14 (93%); 1 cell block yielded a false‐negative result.    CONCLUSIONS:  Cytologic direct smears represent a robust and valuable source of cellular material for immunocytochemistry and molecular studies, especially in instances in which inadequate cell block cellularity is anticipated or encountered. Cancer (Cancer Cytopathol) 2012. © 2011 American Cancer Society.     This study demonstrates that direct smears represent a robust and valuable source of cellular material for ancillary studies used in the cytologic diagnosis of melanoma. Direct smears can be effectively used for confirmatory immunocytochemical studies and molecular assays designed to interrogate the  BRAF  mutational status of melanoma, especially in scenarios in which inadequate cell block cellularity is anticipated or encountered
Potential pitfalls for false suspicion of papillary thyroid carcinoma: A Cytohistologic Review of 22 Cases

To evaluate the diagnostic pitfalls attributing to false suspicious interpretation of papillary thyroid carcinoma (PTC), we performed a retrospective cytohistologic review of thyroid nodules diagnosed as suspicious for papillary carcinoma with histology‐proven false suspicion of PTC. A total of 22 thyroid fine needle aspirates along with the corresponding surgical specimens were reviewed. The presence and/or absence of PTC‐associated morphologic features and their mimics were evaluated. One aspirate showed papillary‐like fragments with honeycomb architecture. One aspirate contained rare syncytial‐type fragments which were accompanied by honeycomb sheets of follicular cells and moderate amount of colloid. Intranuclear grooves presented occasionally in virtually all 22 aspirates. The grooves were noted mainly within round nuclei and appeared thin and/or incomplete. Rare intranuclear pseudoinclusions were detected in one otherwise benign‐appearing aspirates. Eight aspirates contained various numbers of histiocytes, as well as atypical polygonal, epithelioid, elongate, or spindle cells in which intranuclear grooves were occasionally seen. Conspicuous fragments of fibrocollagenous tissue with entrapped follicular cells were detected in one aspirate. The corresponding histological specimen revealed counterpart of the cytological findings described. Other features associated with PTC were inconspicuous. The findings demonstrated that the following factors may contribute to the false suspicion of PTC: (1) misinterpretation of papillary‐like tissue fragments with honeycomb arrangement and fragments offibrocollagenous tissue with entrapped follicular cells; (2) overinterpretation of suboptimal intranuclear grooves and rare intranuclear pseudoinclusion in the absence of the other features of PTC; (3) misinterpretation of the polygonal, epithelioid, elongate, or spindle cells that actually represented atypical cyst lining cells. Diagn. Cytopathol. 2012;40:E74–E79.  © 2011 Wiley Periodicals, Inc
CD147 immunohistochemistry discriminates between reactive mesothelial cells and malignant mesothelioma

Malignant mesothelioma (MM) is a rare form of cancer. Its histopathological diagnosis is very difficult, as it exhibits a number of different appearances that can be misinterpreted as metastatic invasion or atypical hyperplasia. Thus, there is an urgent need to identify adequate markers to distinguish between benign and malignant cells, allowing the implementation of appropriate therapies and, possibly, specific directed therapies. MM, like other tumors, show an increase in glucose uptake, due to high rates of glycolysis, inducing an intracellular overload of acids. In this context, monocarboxylate transporters (MCTs) emerge as important players, by mediating the transmembranar co‐transport of lactate with a proton, thereby, regulating pH and allowing continuous glycolysis. Importantly, proper MCT expression and activity depend on its co‐expression with a chaperone, CD147, which is associated with poor prognosis in cancer. Twenty‐two samples including reactive mesothelial cells, MM, and atypical mesothelial hyperplasias were evaluated for immunoexpression of MCT1, MCT4, and CD147. Expression of these proteins was compared with GLUT1 as a new promising marker for MM. Although MCT isoforms were not differentially expressed in the two types of cytological specimens, CD147, as GLUT1, was almost exclusively expressed in MM. Both MCT1 and MCT4 are not able to discriminate between mesothelial reactive cells and mesothelial malignant cells, while CD147 was able to distinguish these two proliferations. If confirmed, besides being a good marker for identification of MM, CD147 may also be a target for therapeutical strategies in this rare type of tumor. Diagn. Cytopathol. 2012;40:478–483. © 2012 Wiley Periodicals, Inc
The application of immunocytochemistry to direct smears in the diagnosis of effusions

Metastatic malignancy represents a common cause of effusions. Immunocytochemistry (ICC) is useful in confirming malignancy and gaining insight into the site of origin. Cell blocks are commonly utilized for this purpose; nonetheless, when the malignant cells are sparse, they may not be represented in cell blocks thereby precluding immunophenotypic characterization. Thus, we sought to investigate the utility of direct smear preparations as a platform for ICC in the diagnosis of effusions. Air‐dried, unstained direct smears were prepared from 49 malignant effusions and 17 reactive effusions for comparison. ICC for EMA and MOC‐31 highlighted the tumor cells in 91 and 98% of the malignant effusions tested, respectively. EMA immunoreactivity was focally observed within the calretinin‐positive mesothelial cell population in 1 (6%) of the 17 reactive effusions. ICC for MOC‐31 was negative in all reactive effusions. Site‐specific immunomarkers were also evaluated. Immunoreactivity for Napsin‐A and TTF‐1 were observed in 78 and 67% of metastatic lung adenocarcinomas, respectively. ICC for PAX8 highlighted metastatic Müllerian and thyroid carcinomas in 100% of cases tested. CDX‐2 immunoreactivity was observed in 25, 60, and 100% of metastatic gastric, pancreatic, and colorectal adenocarcinomas, respectively. Positivity for p63 was observed in 75% of metastatic urothelial cell carcinomas and the one case of pulmonary squamous cell carcinoma examined. Calretinin ICC highlighted the tumor cells in both malignant mesothelioma cases tested as well as the benign mesothelial cells in the reactive effusions. In conclusion, direct smears represent an effective platform for the performance of ICC in the diagnosis of malignant effusions. Diagn. Cytopathol. 2013. © 2012 Wiley Periodicals, Inc
Cytomorphologic features of metastatic urothelial carcinoma in serous effusions

Metastatic urothelial carcinoma (UC) to serous effusion (SE) is extremely rare and its cytomorphological features have only been described in case reports. In this study, we searched the pathology database at University of Michigan for SEs due to metastatic UC in the last 20 years. A total of 25 cases from 20 patients with clinically and pathologically confirmed metastatic UC in SEs were retrieved. The specimens consisted of 15 pleural, 8 peritoneal, and 2 pericardial effusions. Smears were reviewed and evaluated for the following features: cellularity, single cells, cell clusters or short cords, cell wrapping, “windows” between the cells, two‐tone cytoplasm, cytoplasmic vacuoles, signet ring cells, nuclear to cytoplasmic (N/C) ratio, nuclear hyperchromasia, irregular nuclear membrane, nuclear centricity, double or multiple nuclei, nucleoli, anaplastic cells and mitosis. Our results showed that UC manifested in SEs predominantly as a single cell population with or without clusters or short cords, and frequently exhibited the “cell wrapping” of two or more cells. Individual UC cell in SEs exhibited nuclear enlargement with increased N/C ratio, irregular nuclear membranes, hyperchromatic coarse chromatin and frequently prominent nucleoli. Double or multinucleated cells, cells with vacuolated cytoplasm or signet ring appearance were also frequently present. Our results demonstrated that while certain features could suggest the diagnosis of UC, the cytomorphological features are not specific and often overlap with those of reactive mesothelium, mesothelioma, metastatic adenocarcinoma, or squamous cell carcinoma in SEs. Accurate diagnosis of UC rests on the combination of clinical history, cytomorphologic features and appropriate immunohistochemical panel. Diagn. Cytopathol. 2013. © 2012 Wiley Periodicals, Inc
Deciduoid mesothelioma: Cytologic presentation and diagnostic pitfalls

We report two cases of malignant deciduoid mesothelioma (MDM), a very rare variant of malignant mesothelioma (MM). Case 1: An 18‐year‐old male with no history of asbestos exposure presented with worsening abdominal pain, anorexia, and vomiting after a motor vehicle accident. A CT scan showed small amount of ascites and abdominal mass. An exploratory laparotomy revealed multiple yellow tan, firm nodules on the peritoneum and omentum. He received palliative treatment and died 5 months after the diagnosis. Case 2: A 64‐year‐old female with history of asbestos exposure initially presented with abdominal distension. CT scan showed abdominal mass with a large amount of ascites. A diagnostic laparoscopy revealed multiple peritoneal nodules. She underwent several regimens of chemotherapy over a period of 69 months and is still alive to date. In both cases, features of mesothelial origin were subtle and the smears showed predominantly single cells with marked nuclear atypia. The second case also contained few two‐dimensional loose cell clusters with scalloped or hobnail borders. The clusters often exhibited a pseudoacinar structure surrounding a globular extracellular material. Groups of three to four cells often formed doublets and triplets with cell‐to‐cell windows.  Our results show that MDM may not present with the traditional cytological features described in MM and can manifest with more nuclear pleomorphism resulting in erroneous diagnosis. Recognition of the subtle mesothelial features along with the appropriate ancillary tests is essential for accurate diagnosis. Diagn. Cytopathol. 2013. © 2012 Wiley Periodicals, Inc
Fine-needle aspiration cytology of Merkel cell carcinoma-A review of 69 cases

Background\ud
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This study reviewed the clinical presentation, cytologic findings and the immunophenotype of 69 Merkel Cell Carcinoma (MCC) cases sampled by FNA.\ud
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Methods\ud
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Demographic and clinical data, the cytology findings and results of ancillary testing were reviewed. \ud
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Results\ud
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Median patient age was 78 years (37 – 104) with a 1:1.8 female to male ratio. The most common FNA sites sampled included lymph nodes in the neck, the axillary region, the inguinal region and the  parotid gland. Most patients had a history of MCC (68%) &/or non-MCC malignancy (70%). \ud
The common cytologic pattern was a cellular smear with malignant cells arranged in a dispersed pattern with variable numbers of disorganised groups of cells. Cytoplasm was scant or absent and nuclei showed mild to moderate anisokaryosis, stippled chromatin, inconspicuous nucleoli and nuclear molding. Numerous apoptotic bodies were often present.\ud
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Cell block samples (28 cases) were usually positive for cytokeratins in a perinuclear dot pattern, including 88% of cases with CK20 positivity. CD56 was the most sensitive (95%) neuroendocrine marker on cell blocks and was also positive with flow cytometry in 9 cases tested.\ud
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Conclusions\ud
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MCC is most commonly seen in FNA specimens from the head and neck of elderly patients, often with a history of previous skin lesions. Occasional cases present in younger patients and some may be mistaken for other round blue cell tumors, such as lymphoma. CD 56 may be a useful marker in cell block preparations and in flow cytometric analysis of MCC
Molecular diagnosis of infectious diseases using cytological specimens

Pathologists have an important role in the diagnosis of infectious disease (ID). In many cases, a definitive diagnosis can be made using cytopathology alone. However, several ancillary techniques can be used on cytological material to reach a specific diagnosis by identifying the causative agent and consequently defining the management of the patient. This review aims to present the effectiveness of the application of molecular studies on cytological material to diagnose IDs and discuss the advantages and disadvantages of the various molecular techniques according to the type of cytological specimen and the infectious agents.info:eu-repo/semantics/publishedVersio
Lung cancer samples preserved in liquid medium: One step beyond cytology

Lung cancer is one of the most common cancer types in men and women worldwide with a high mortality rate. World Health Organization (WHO) classification has accepted biopsy as the primary sample for lung cancer diagnosis, pathological classification and molecular testing for management of patients, yet, the use of alternative sampling procedures is highly encouraged. Bronchial cytological samples require a less invasive collection technique and may be suitable for pathological and molecular analysis and storage in liquid medium. Furthermore, the molecular analysis of bronchial cytological samples allows the detection of molecular biomarkers, which may be useful for the selection of molecular targeted therapies. Thus, the purpose of this review is to describe the usefulness of bronchial cytological samples preserved in liquid medium from lung cancer patients for pathological diagnosis and molecular investigation.Rui Manuel Reis and Adhemar Longatto-Filho have a Brazilian National Counsel of Technological and Scientific Development (CNPq) Produtivity scholarship.info:eu-repo/semantics/publishedVersio
Vaginal Enterobius vermicularis diagnosed on liquid-based cytology during Papanicolaou test cervical cancer screening: A report of two cases and a review of the literature.

Enterobiasis is one of the most common human parasitic infections. It is considered an intestinal parasite, but cases of extra-intestinal affections exist, notably infections of the female genital tract. Enterobius vermicularis (EV) eggs (or ova) have been found in the cervical smears of two patients in our institute during the last 16 years. No gynaecological or gastrointestinal symptoms were reported, and there was no known intestinal infection in these two cases. A review of the available literature revealed rare cases of vaginal enterobiasis, with a wide range of clinical presentations, many patients being asymptomatic. The diagnosis may sometimes be difficult, mainly because of the lack of clinical suspicion. However, cytological identification of EV in cervico-vaginal smears is important, especially when considering the risk of ascending infections of the genital tract associated with severe complications
Carbohydrate-derived fulvic acid (CHD-FA) inhibits carrageenan-induced inflammation and enhances wound healing : efficacy and toxicity study in rats

The objectives of this study were to evaluate the safety and anti-inflammatory and wound healing characteristics of carbohydrate derived fulvic acid (CHD-FA) in rats. A daily oral dosage of CHD-FA at 100mg/kg and higher effectively reduced carrageenan-induced
paw oedema in rats which was comparable to an oral dosage of 10mg/kg indomethacin. Furthermore, the topical application of CHD-FA formulated to contain 1.75% active product in an cetomicrogol cream at pH 1.98, compared favourably with fusidic acid cream (10mg/g) in accelerating the healing of excised wounds infected with S. aureus. No signs of toxicity were observed in rats during the 6-day acute and 6 month chronic oral treatment with CHD-FA at
100mg/Kg bodyweight. Topical application of CHD-FA, formulated in UEA cream and applied to the right ears of mice at 400mg/Kg bodyweight on days one, and 7 to 38 produced no adverse events. No signs of toxicity were observed in the teratogenicity study where CHD-FA was administered at 100mg/kg bodyweight to pregnant female mice by gavage 3 days before fertilization to 14 days of pregnancy.
In conclusion, CHD-FA is a safe compound with anti-inflammatory and wound healing properties and merits further evaluation in the treatment of patients suffering from similar conditions.Fulvimed (Pty) Ltd, The Technology and Human Resources for Industry Programme of the National Research Foundation and the Department of Trade and Industry (THRIP)http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1098-2299dm201
Drainage network reorganization affecting the Nene and Welland catchments of eastern England as a result of a late Middle Pleistocene glacial advance

Cycladic Archaeology and Research: New approaches and discoveries reflects the present exciting times in Cycladic archaeology. New excavations are bringing to light sanctuaries unmentioned by literary sources and inscriptions (e.g., Kythnos, Despotiko); new theoretical approaches to insularity and networks are radically changing our views of the Cyclades as geographic and cultural unit(s). Furthermore, the restoration and restudy of older sites (e.g., Delos, Paros, Naxos) are challenging old truths, updating chronologies and contexts throughout the Mediterranean and beyond. This volume is intended to share these recent developments with a broader, international audience. The essays have been carefully selected as representing some of the most important recent work and include significant previously-unpublished material. Individually, they cover archaeological sites and materials from across the Cycladic islands, and illustrate the diversity of the islands’ material culture across the Geometric, Archaic, Classical, Hellenistic, Roman, and Late Antique periods. Together, they share common themes such as the importance of connectivity, and the role of each island’s individual landscape and its resources in shaping human activity. The work they represent attests the ongoing appeal of the islands and of the islanders in the collective imagination, and demonstrates the scope for still further innovative work in the years ahead
Long‐term evolution of the Old Rhine estuary : unraveling effects of changing boundary conditions and inherited landscape.

The long‐term morphodynamic evolution of estuaries depends on a combination of antecedent topography and boundary conditions, including fluvial input, sea‐level change and regional‐landscape interactions. Identifying effects of such boundary conditions on estuary evolution is important to anticipate future changes in specific boundary conditions and for hindcasting with numerical and physical models. A comprehensive synthesis of the evolution of the former Old Rhine estuary is presented here, together with its boundary conditions over its full lifespan from 6,500 to 1,000 cal. yr bp. This system formed during a period of sea‐level high stand, during which the estuary served as the main River Rhine outlet. The estuary went through three stages of evolution: a maturation phase in a wide infilling back‐barrier basin, a stable mature phase and an abandoning phase, both in a laterally confined setting. The Old Rhine River formed by a river avulsion around 6,500 cal. yr bp that connected to a tidal channel within a large back‐barrier basin. Decelerating sea‐level rise caused the back‐barrier basin to silt up around 5,700 cal. yr bp, resulting in shoreline progradation by beach‐barrier formation until ∼2,000 cal. yr bp. Beach‐barrier formation along the coast and natural levee formation along the river triggered peat formation in the coastal plain, laterally constraining the estuary and limiting overbank deposition, which caused most sediment to accumulate offshore. The abandoning phase started around 2,200 cal. yr bp when a series of upstream avulsions led to a substantial reduction in fluvial input. This induced a period of enhanced estuarine overbank clay deposition that continued into near‐complete silting up and estuary closure around 1200 ad. These findings exemplify how tidal systems, formed in wide coastal plains during sea‐level high stand, depend on antecedent conditions, and how they respond to connection and disconnection of a large river over long, millennial timescales