pinecone/core-2020-05-10-deduplication huggingface dataset:
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Maurocalcine transduction into cells

International audienceMaurocalcine (MCa) is a 33-amino-acid residue peptide toxin isolated from the scorpion Scorpio maurus palmatus. External application of MCa to cultured myotubes is known to produce Ca2+ release from intracellular stores. MCa binds directly to the skeletal muscle isoform of the ryanodine receptor, an intracellular channel target of the endoplasmic reticulum, and induces long lasting channel openings in a mode of smaller conductance. Here we investigated the way MCa proceeds to cross biological membranes to reach its target. A biotinylated derivative of MCa was produced (MCa(b)) and complexed with a fluorescent indicator (streptavidine-cyanine 3) to follow the cell penetration of the toxin. The toxin complex efficiently penetrated into various cell types without requiring metabolic energy (low temperature) or implicating an endocytosis mechanism. MCa appeared to share the same features as the so-called cell-penetrating peptides. Our results provide evidence that MCa has the ability to act as a molecular carrier and to cross cell membranes in a rapid manner (1-2 min), making this toxin the first demonstrated example of a scorpion toxin that translocates into cells
The transportation sector and low-carbon growth pathways: modeling urban, infrastructure and spatial determinants of mobility

International audienceThere is still a controversy as to the effect of spatial organization on CO2 emissions. This paper contributes to this debate by investigating the potentials offered by infrastructure measures favoring lower mobility in the transition to a low-carbon economy. This is done by embarking a detailed description of passenger and freight transportation in an energy-economy-environment (E3) model. In addition to the standard representation of transport technologies, this framework considers explicitly the "behavioural" determinants of mobility that drive the demand for transport but are often disregarded in mitigation assessments: constrained mobility needs (essentially commuting) imposed by the spatial organization of residence and production, modal choices triggered by installed infrastructure and the freight transport intensity of production processes. This study demonstrates that the implementation of measures fostering a modal shift towards low-carbon modes and a decoupling of mobility needs from economic activity significantly modifies the sectoral distribution of mitigation efforts and reduces the carbon tax levels necessary to reach a given climate target relatively to a "carbon price only" policy. This result is robust to a wide range of assumptions about exogenous parameters
Traveler-Oriented Advanced Traveler Information System Based on Discovery and Exploitation of Resources: Potentials and Challenges

International audienceAn Advanced Traveler Information System (ATIS) aims at providing travelers with multi-modal trip planning, route guidance services and advisory functions. Most ATIS’s provide travel solutions that cover only specific geographic areas, travel modes and/or transport operators, and are insufficiently personalized. Moreover, the solutions are normally constructed upon a fixed set of resources (e.g. data sources and services), and thus are not highly adaptable to travelers’ diverse needs. Considering numerous existing ATIS’s and other resources on the Web, our vision is to use them complementarily to improve the adaptability, completeness, and personalization of the solutions. We envision an ATIS design, called traveler-oriented ATIS, that discovers and exploits web resources in an integrated manner to construct travel solutions tailored to travelers’ needs. In this paper, we discuss common approaches to managing resources, upon which the solutions are built, employed in various ATIS’s and their limitations that lead to our vision. Challenges of the vision are investigated, and a possible approach to address them is presented along with potential applications of Semantic Web and Multi-Agent Systems in the approach to demonstrate a promising direction to concretize the traveler-oriented ATIS design
Determination of binary asteroid orbits with a genetic-based algorithm

Reproduced with permission. Copyright ESO. The original publication is available at www.aanda.org.International audienceAims: Over the past decade, discoveries of multiple and binary asteroid systems have played a significant role in our general understanding of small solar system bodies. Direct observations of satellites of asteroids are rare and difficult since they require the use of already over-subscribed facilities such as adaptive optics (AO) on large 8-10 m class telescopes and the Hubble Space Telescope (HST). The scarcity of data and the long temporal baseline of observations (up to 10 years) significantly complicate the determination of the mutual orbits of these systems. Methods: We implemented a new approach for determining the mutual orbits of directly-imaged multiple asteroids using a genetic-based algorithm. This approach was applied to several known binary asteroid systems (22 Kalliope, 3749 Balam, and 50 000 Quaoar) observed with AO systems and HST. This statistical method is fast enough to permit the search for an orbital solution across a large parameter space and without a priori information about the mutual orbit. Results: From 10 years of observation, we derived an orbital solution for Linus, companion of (22) Kalliope, with an accuracy close to the astrometric limit provided by the AO observations, assuming a purely Keplerian orbit. A search for non-Keplerian orbit confirmed that a J2 ~ 0 is the best-fitting solution. We show that the precession of the nodes could be detected without ambiguity, implying that Kalliope's primary may have an inhomogeneous internal structure. HST astrometric observations of Weywot, companion of the trans-Neptunian object (50 000) Quaoar, were used to derive its mass and its bulk density, which appears to be higher than the density of other TNOs. Finally, we derived a bundle of orbital solutions for (3749) Balam, with equally good fits, from the limited set of astrometric positions. They provide a realistic density between 1.3 and 3.7 g/cm3 for this S-type asteroid
First observation of CO at 345 GHz in the atmosphere of Saturn with the JCMT. New constaints on its origin

International audienceWe have performed the first observation of the CO(3-2) spectral line in the atmosphere of Saturn with the James Clerk Maxwell Telescope. We have used a transport model of the atmosphere of Saturn to constrain the origin of the observed CO. The CO line is best-fit when the CO is located at pressures less than (15± 2) mbar with a mixing ratio of (2.5±0.6)×10-8 implying an external origin. By modelling the transport in Saturn's atmosphere, we find that a cometary impact origin with an impact 200-350 years ago is more likely than continuous deposition by interplanetary dust particles (IDP) or local sources (rings/satellites). This result would confirm that comet impacts are relatively frequent and efficient providers of CO to the atmospheres of the outer planets. However, a diffuse and/or local source cannot be rejected, because we did not account for photochemistry of oxygen compounds. Finally, we have derived an upper limit of 1×10-9 on the tropospheric CO mixing ratio
On preferred axes in WMAP cosmic microwave background data after subtraction of the integrated Sachs-Wolfe effect

International audienceThere is currently a debate over the existence of claimed statistical anomalies in the cosmic microwave background (CMB), recently confirmed in Planck data. Recent work has focussed on methods for measuring statistical significance, on masks and on secondary anisotropies as potential causes of the anomalies. We investigate simultaneously the method for accounting for masked regions and the foreground integrated Sachs-Wolfe (ISW) signal. We search for trends in different years of WMAP CMB data with different mask treatments. We reconstruct the ISW field due to the 2 Micron All-Sky Survey (2MASS) and the NRAO VLA Sky Survey (NVSS) up to ℓ = 5, and we focus on the Axis of Evil (AoE) statistic and even/odd mirror parity, both of which search for preferred axes in the Universe. We find that removing the ISW reduces the significance of these anomalies in WMAP data, though this does not exclude the possibility of exotic physics. In the spirit of reproducible research, all reconstructed maps and codes will be made available for download at http://www.cosmostat.org/anomaliesCMB.html
Mechanism and regulation of the Two-component FMN-dependent monooxygenase ActVA-ActVB from Streptomyces coelicolor.

International audienceThe ActVA-ActVB system from Streptomyces coelicolor is a two-component flavin-dependent monooxygenase involved in the antibiotic actinorhodin biosynthesis. ActVB is a NADH:flavin oxidoreductase that provides a reduced FMN to ActVA, the monooxygenase that catalyzes the hydroxylation of dihydrokalafungin, the precursor of actinorhodin. In this work, using stopped-flow spectrophotometry, we investigated the mechanism of hydroxylation of dihydrokalafungin catalyzed by ActVA and that of the reduced FMN transfer from ActVB to ActVA. Our results show that the hydroxylation mechanism proceeds with the participation of two different reaction intermediates in ActVA active site. First, a C(4a)-FMN-hydroperoxide species is formed after binding of reduced FMN to the monooxygenase and reaction with O(2). This intermediate hydroxylates the substrate and is transformed to a second reaction intermediate, a C(4a)-FMN-hydroxy species. In addition, we demonstrate that reduced FMN can be transferred efficiently from the reductase to the monooxygenase without involving any protein.protein complexes. The rate of transfer of reduced FMN from ActVB to ActVA was found to be controlled by the release of NAD(+) from ActVB and was strongly affected by NAD(+) concentration, with an IC(50) of 40 microm. This control of reduced FMN transfer by NAD(+) was associated with the formation of a strong charge.transfer complex between NAD(+) and reduced FMN in the active site of ActVB. These results suggest that, in Streptomyces coelicolor, the reductase component ActVB can act as a regulatory component of the monooxygenase activity by controlling the transfer of reduced FMN to the monooxygenase
Micro-crystalline inclusions analysis by PIXE and RBS

International audienceA characteristic feature of the nuclear microprobe using a 3 MeV proton beam is the long range of particles (around 70 μm in light matrices). The PIXE method, with EDS analysis and using the multilayer approach for treating the X-ray spectrum allows the chemistry of an intra-crystalline inclusion to be measured, provided the inclusion roof and thickness at the impact point of the beam (Z and e, respectively) are known (the depth of the inclusion floor is Z + e). The parameter Z of an inclusion in a mineral can be measured with a precision of around 1 μm using a motorized microscope. However, this value may significantly depart from Z if the analyzed inclusion has a complex shape. The parameter e can hardly be measured optically. By using combined RBS and PIXE measurements, it is possible to obtain the geometrical information needed for quantitative elemental analysis. This paper will present measurements on synthetic samples to investigate the advantages of the technique, and also on natural solid and fluid inclusions in quartz. The influence of the geometrical parameters will be discussed with regard to the concentration determination by PIXE. In particular, accuracy of monazite micro-inclusion dating by coupled PIXE-RBS will be presented
Stability and turbulent transport in Taylor–Couette flow from analysis of experimental data

International audienceThis paper provides a prescription for the turbulent viscosity in rotating shear flows for use e.g. in geophysical and astrophysical contexts. This prescription is the result of the detailed analysis of the experimental data obtained in several studies of the transition to turbulence and turbulent transport in Taylor-Couette flow. We first introduce a new set of control parameters, based on dynamical rather than geometrical considerations, so that the analysis applies more naturally to rotating shear flows in general and not only to Taylor-Couette flow. We then investigate the transition thresholds in the supercritical and the subcritical regime in order to extract their general dependencies on the control parameters. The inspection of the mean profiles provides us with some general hints on the mean to laminar shear ratio. Then the examination of the torque data allows us to propose a decomposition of the torque dependence on the control parameters in two terms, one completely given by measurements in the case where the outer cylinder is at rest, the other one being a universal function provided here from experimental fits. As a result, we obtain a general expression for the turbulent viscosity and compare it to existing prescription in the literature. Finally, throughout all the paper we discuss the influence of additional effects such as stratification or magnetic fields
COSIMA-Rosetta calibration for in-situ characterization of 67P/Churyumov-Gerasimenko cometary inorganic compounds

20 pages, 3 figures, 5 tablesInternational audienceCOSIMA (COmetary Secondary Ion Mass Analyser) is a time-of-flight secondary ion mass spectrometer (TOF-SIMS) on board the Rosetta space mission. COSIMA has been designed to measure the composition of cometary dust grains. It has a mass resolution m/{\Delta}m of 1400 at mass 100 u, thus enabling the discrimination of inorganic mass peaks from organic ones in the mass spectra. We have evaluated the identification capabilities of the reference model of COSIMA for inorganic compounds using a suite of terrestrial minerals that are relevant for cometary science. Ground calibration demonstrated that the performances of the flight model were similar to that of the reference model. The list of minerals used in this study was chosen based on the mineralogy of meteorites, interplanetary dust particles and Stardust samples. It contains anhydrous and hydrous ferromagnesian silicates, refractory silicates and oxides (present in meteoritic Ca-Al-rich inclusions), carbonates, and Fe-Ni sulfides. From the analyses of these minerals, we have calculated relative sensitivity factors for a suite of major and minor elements in order to provide a basis for element quantification for the possible identification of major mineral classes present in the cometary grains
Quasiperiodic emissions observed by the Cluster spacecraft and their association with ULF magnetic pulsations

International audience[1] Quasiperiodic (QP) emissions are electromagnetic waves at frequencies of about 0.5–4 kHz characterized by a periodic time modulation of the wave intensity, with a typical modulation period on the order of minutes. We present results of a survey of QP emissions observed by the Wide-Band Data (WBD) instruments on board the Cluster spacecraft. All WBD data measured in the appropriate frequency range during the first 10 years of operation (2001–2010) at radial distances lower than 10 R E were visually inspected for the presence of QP emissions, resulting in 21 positively identified events. These are systematically analyzed, and their frequency ranges and modulation periods are determined. Moreover, a detailed wave analysis has been done for the events that were strong enough to be seen in low-resolution Spatio-Temporal Analysis of Field Fluctuations-Spectrum Analyzer data. Wave vectors are found to be nearly field-aligned in the equatorial region, but they become oblique at larger geomagnetic latitudes. This is consistent with a hypothesis of unducted propagation. ULF magnetic field pulsations were detected at the same time as QP emissions in 4 out of the 21 events. They were polarized in the plane perpendicular to the ambient magnetic field, and their frequencies roughly corresponded to the modulation period of the QP events. Citation: Němec , F., O. Santolík, J. S. Pickett, M. Parrot, and N. Cornilleau-Wehrlin (2013), Quasiperiodic emissions observed by the Cluster spacecraft and their association with ULF magnetic pulsations
Tensor decomposition exploiting diversity of propagation velocities; application to localization of icequake events

International audienceThe problem of direction of arrival (DoA) estimation of seismic plane waves impinging on an array of sensors is considered from a new deterministic perspective using tensor decomposition techniques. In addition to temporal and spatial sampling, further information is taken into account, based on the different propagation speed of  body  waves (P and S) through solid media. Performances are evaluated through simulated data in terms of the Cramér-Rao bounds and compared to other reference methods such as ESPRIT and MUSIC, in the presence of additive Gaussian circular noise. The proposed approach is then applied to real seismic data recorded at the Argentière glacier, occurring at the interface between the ice mass and the underlying bedrock.  MUSIC and ESPRIT  rely on the estimation of the covariance matrix of received data, thus requiring a large number of time samples. Moreover, information about propagation speed diversity is not taken into account by existing models in array processing. The discovered advantage in terms of the average error in estimating the direction of arrival of body waves is noteworthy, especially for a low number of sensors, and in separating closely located sources. Additionally, an improvement of precision in processing real seismic data is observed
Does convective aggregation need to be represented in cumulus parameterizations?

1942-2466Tropical deep convection exhibits a variety of levels of aggregation over a wide range of scales. Based on a multisatellite analysis, the present study shows at mesoscale that different levels of aggregation are statistically associated with differing large-scale atmospheric states, despite similar convective intensity and large-scale forcings. The more aggregated the convection, the dryer and less cloudy the atmosphere, the stronger the outgoing longwave radiation, and the lower the planetary albedo. This suggests that mesoscale convective aggregation has the potential to affect couplings between moisture and convection and between convection, radiation, and large-scale ascent. In so doing, aggregation may play a role in phenomena such as "hot spots" or the Madden-Julian Oscillation. These findings support the need for the representation of mesoscale organization in cumulus parameterizations; most parameterizations used in current climate models lack any such representation. The ability of a cloud system-resolving model to reproduce observed relationships suggests that such models may be useful to guide attempts at parameterizations of convective aggregation
Si Isotope Homogeneity of the Solar Nebula

International audienceThe presence or absence of variations in the mass-independent abundances of Si isotopes in bulk meteorites provides important clues concerning the evolution of the early solar system. No Si isotopic anomalies have been found within the level of analytical precision of 15 ppm in 29Si/28Si across a wide range of inner solar system materials, including terrestrial basalts, chondrites, and achondrites. A possible exception is the angrites, which may exhibit small excesses of 29Si. However, the general absence of anomalies suggests that primitive meteorites and differentiated planetesimals formed in a reservoir that was isotopically homogenous with respect to Si. Furthermore, the lack of resolvable anomalies in the calcium-aluminum-rich inclusion measured here suggests that any nucleosynthetic anomalies in Si isotopes were erased through mixing in the solar nebula prior to the formation of refractory solids. The homogeneity exhibited by Si isotopes may have implications for the distribution of Mg isotopes in the solar nebula. Based on supernova nucleosynthetic yield calculations, the expected magnitude of heavy-isotope overabundance is larger for Si than for Mg, suggesting that any potential Mg heterogeneity, if present, exists below the 15 ppm level
Numerical assessment of 3-D macrodispersion in heterogeneous porous media

International audienceHydrodynamic dispersion is a key controlling factor of solute transport in heterogeneous porous media that critically depends on dimensionality. The transverse macrodispersion (asymptotic dispersion transverse to the mean velocity direction) is known to vanish only in 2-D and not in 3-D. Using classical Gaussian correlated permeability fields with a lognormal distribution of variance inline image, we determine numerically the longitudinal and transverse dispersivities as functions of heterogeneity and dimensionality. We show that the transverse macrodispersion steeply increases with inline image underlying the essential role of flow lines braiding, a mechanism specific to 3-D systems that we qualitatively characterize by the increasing expansion of the flow lines transversally to the flow direction. The transverse macrodispersion remains however at least two orders of magnitude smaller than the longitudinal macrodispersion, which increases even more steeply with inline image. At moderate to high levels of heterogeneity, the transverse dispersion also converges much faster to its asymptotic regime than do the longitudinal dispersion. Braiding cannot be thus taken as the sole mechanism responsible for the high longitudinal macrodispersions. It could be either supplemented or superseded by stronger velocity correlations in 3-D than in 2-D. This assumption is supported by the much larger longitudinal macrodispersions obtained in 3-D than in 2-D, up to a factor of 5 for inline imag