pinecone/core-2020-05-10-deduplication huggingface dataset:
Deficit irrigation and maturation stage influence quality and flavonoid composition of ‘Valencia’ orange fruit

BACKGROUND:Effects of continuous deficit irrigation (DI) and partial rootzone drying (PRD) treatments (50%ETc) in comparison\ud
with full irrigation (CI, 100% ETc) were investigated during ‘Valencia’ orange fruit maturation. Ultra-high-performance liquid\ud
chromatography/high-resolutionmass spectrometry was used to quantify hesperidin, narirutin, tangeritin, nobiletin, didymin\ud
and neoeriocitrin in the fruit juice and peel.\ud
RESULTS: No significant effect of irrigation was found on yield, juice soluble solids or acidity. Juice color was not influenced\ud
by irrigation or harvest date, whereas peel color increased during maturation and was more pronounced in CI and PRD fruits.\ud
Juice acidity reached a peak in May, while soluble solids increased linearly throughout maturation. Hesperidin was the major\ud
flavanone detected during maturation, with concentrations 200-fold higher in the fruit peel than in the juice. In the peel,\ud
narirutin, didymin and neoeriocitrin decreased while hesperidin, nobiletin and tangeritin increased with maturation. Narirutin\ud
synthesis in the orange fruit was insensitive to irrigation strategy. In fruit peels, PRD and DI induced the decline of hesperidin,\ud
nobiletin and tangeritin only in June, whereas in the juice, deficit irrigation treatments induced an increase in hesperidin and\ud
didymin.\ud
CONCLUSION: These results suggest that deficit irrigation, in particular the conditions imposed with PRD, may cause a\ud
significant accumulation shift of total flavonoids from the fruit peel into the juice, with a positive impact on juice quality and\ud
nutritional value. Fruit compositional changes during maturation also suggest that late harvest can improve fruit palatability\ud
and nutritional quality under the cultural and environmental conditions of this study
Evaluation of polyphenolic content and antioxidant activity in two onion varieties grown under organic and conventional production systems

BACKGROUND: Onions contain a number of bioactive compounds, in particular polyphenols. They are rich sources of such compounds in the human diet and offer significant health benefits to the consumer. Demand for organic crops is steadily increasing partly based on the expected health benefits of organic food consumption. The current study examines the influence of organic and conventional crop management practices on bioactive polyphenolic content of onion. RESULTS: We examined the effect of conventional, organic, and mixed cultivation practices on the content of total phenolics, total flavonoids and antioxidant activity in two varieties of onion grown over 4 years in a split-plot factorial systems comparison trial. Levels of total phenolics and total flavonoids showed a significant year-on-year variation and were significantly different between organic and conventional production systems. The levels of total phenolics, total flavonoids and antioxidant activity in general were significantly higher (P < 0.05) under fully organic compared to fully conventional management. CONCLUSION: Organic cultivation practices resulted in significantly higher levels of potential bioactive compounds in onion
Antioxidant properties and global metabolite screening ofthe probiotic yeast Saccharomyces cerevisiae var. boulardii

BACKGROUND: Saccharomyces cerevisiae var. boulardii is the only yeast species with probiotic properties. It is considered to have therapeutic significance in gastro-intestinal disorders. In this paper, a comparative physiological study between this yeast and Saccharomyces cerevisiae (BY4742) was performed by evaluating two prominent traits of probiotic species, responses to different stress conditions and antioxidant capacity. A global metabolite profile was also developed in order to identify which therapeutically important secondary metabolites are produced.\ud
RESULTS: S. cerevisiae var. boulardii showed no significant difference in growth patterns but greater stress tolerance when compared with S. cerevisiae. It also demonstrated a 6-10-fold greater antioxidant potential (judged by the DPPH assay), with 70-fold higher total phenolic content and 20-fold higher total flavonoid content in the extracellular fraction. These features were clearly differentiated by principal component analysis and further elucidated by metabolite profiling. The extracellular fraction of the S. cerevisiae var. boulardii cultures was found to be rich in polyphenolic metabolites viz. vanillic acid, cinnamic acid, phenyl ethyl alcohol (rose oil), erythromycin, amphetamine and vitamin B6 which results in the antioxidant capacity of this strain.\ud
CONCLUSION: This study presents a new perspective of differentiating the two genetically related strains of yeast, Saccharomyces cerevisiae and Saccharomyces cerevisiae var. boulardii by assessing their metabolome fingerprints. In addition to the correlation of the phenotypic properties with the secretory metabolites of these two yeasts, the work also emphasizes the potential to exploit S. cerevisiae var. boulardii in the industrial production of these metabolites
Investigation into the role of endogenous abscisic acid during ripening of imported avocado cv. Hass

BACKGROUND
The importance of ethylene in avocado ripening has been extensively studied. In contrast, little is known about the possible role of abscisic acid (ABA). The present work studied the effect of 1-methylcyclopropene (1-MCP) (0.3 μL L−1), e+® Ethylene Remover and the combination thereof on the quality of imported avocado cv. Hass fruit stored for 7 days at 12 °C. Ethylene production, respiration, firmness, colour, heptose (C7) sugars and ABA concentrations in mesocarp tissue were measured throughout storage.
RESULTS
Treatment with e+® Ethylene Remover reduced ethylene production, respiration rate and physiological ripening compared with controls. Fruit treated with 1-MCP + e+® Ethylene Remover and, to a lesser extent 1-MCP alone, had the lowest ethylene production and respiration rate and hence the best quality. Major sugars measured in mesocarp tissue were mannoheptulose and perseitol, and their content was not correlated with ripening parameters. Mesocarp ABA concentration, as determined by mass spectrometry, increased as fruit ripened and was negatively correlated with fruit firmness.
CONCLUSIONS
Results suggest a relationship between ABA and ethylene metabolism since blocking ethylene, and to a larger extent blocking and removing ethylene, resulted in lower ABA concentrations. Whether ABA influences avocado fruit ripening needs to be determined in future research. © 2017 Society of Chemical Industr
Dry matter yield, chemical composition and estimated extractable protein of legume and grass species during the spring growth

BACKGROUND: Knowledge of the variation of extractable protein amount in legumes and grasses as affected by harvest time is important for identifying optimal combinations to enable a high protein production in a biorefinery. The extractability of protein was estimated using the Cornell Net Carbohydrate and Protein System across six harvests during the spring growth.\ud
RESULTS: The estimated extractable protein [g kg−1 dry matter (DM)] defined as the easily available fractions B1+B2 was significantly higher in white clover and lucerne at all harvests while, if the more cell wall attached fraction B3 can be extracted, white clover had the highest extractable protein amongst all species. Total yield of B1+B2 per ha was higher in white clover and red clover at the early growth while B1+B2+B3 was by far the highest for red clover through all harvests.\ud
CONCLUSION:White clover could be a good candidate for protein production purpose in a biorefinery due to its high extractable protein content per kg DM. In order to maximise the protein production capacity, harvest should take place during early growth due to a decline in protein extractability with maturity. The final economy of the concept will depend on the value of the fibre after extraction of the protein
A comparison of the phenolic profile and antioxidant activity of different Cichorium spinosum L. ecotypes

Wild greens are considered a rich source of phenolic compounds and antioxidants and an essential part of the so-called Mediterranean diet. In the present study, Cichorium spinosum L. ecotypes, cultivated or collected in situ from wild plants from the eastern Mediterranean, were evaluated regarding their phenolic composition and antioxidant activity. RESULTS: Significant differences were observed among the various studied ecotypes regarding their phenolic compound content and profile, especially between wild and cultivated ecotypes, as well as the phenolic acid content between commercial products and cultivated plants. The antioxidant activity also varied among the various studied ecotypes and growing conditions, with commercial products having the highest antioxidant activity, whereas wild ecotypes showed lower antioxidant activity. CONCLUSION: Cichorium spinosum leaves are a rich source of chicoric and 5-O-caffeoylquinic acid, while significant differences in total phenolic acids, flavonoids and phenolic compound content and in antioxidant activity were observed among the studied ecotypes, as well as between the tested growing conditions. According to the results of the present study, further valorization of C. spinosum species has great potential, since it could be used as a new alternative species in the food industry. © 2017 Society of Chemical Industry.info:eu-repo/semantics/publishedVersio
Targeting carbon for crop yield and drought resilience

Current methods of crop improvement are not keeping pace with projected increases in population growth. Breeding, focused around key traits of stem height and disease resistance, delivered the step‐change yield improvements of the green revolution of the 1960s. However, subsequently, yield increases through conventional breeding have been below the projected requirement of 2.4% per year required by 2050. Genetic modification (GM) mainly for herbicide tolerance and insect resistance has been transformational, akin to a second green revolution, although GM has yet to make major inroads into intrinsic yield processes themselves. Drought imposes the major restriction on crop yields globally but, as yet, has not benefited substantially from genetic improvement and still presents a major challenge to agriculture. Much still has to be learnt about the complex process of how drought limits yield and what should be targeted. Mechanisms of drought adaptation from the natural environment cannot be taken into crops without significant modification for the agricultural environment because mechanisms of drought tolerance are often in contrast with mechanisms of high productivity required in agriculture. However, through convergence of fundamental and translational science, it would appear that a mechanism of sucrose allocation in crops can be modified for both productivity and resilience to drought and other stresses. Recent publications show how this mechanism can be targeted by GM, natural variation and a new chemical approach. Here, with an emphasis on drought, we highlight how understanding fundamental science about how crops grow, develop and what limits their growth and yield can be combined with targeted genetic selection and pioneering chemical intervention technology for transformational yield improvements. © 2017 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry
Minimising food waste: a call for multidisciplinary research

Food losses and waste has always been a significant global problem for mankind, and one which has become increasingly recognised as such by policy makers, food producers, processors, retailers, and consumers. It is however an emotive subject whereby the extent, accuracy, and resolution of available data on postharvest loss and waste is questionable such that key performance indicators on waste can be misinformed. The nature and extent of food waste differs amongst developed economies, economies in transition, and developing economies. Whilst most emphasis has been put on increasing future crop production, far less resource has been and is still channelled towards enabling both established and innovative food preservation technologies to reduce food waste while maintaining safety and quality. Reducing food loss and waste is a more tractable problem than increasing production in the short to medium term, as its solution is not directly limited, for instance, by available land and water resources. Here we argue the need for a paradigm shift of current funding strategies and research programmes which will encourage the development, implementation, and translation of collective biological, engineering, and management solutions to better preserve and utilise food. Such cross disciplinary thinking across global supply chains is an essential element in the pursuit of sustainable food and nutritional security. The implementation of allied technological and management solutions is reliant on there being sufficient skilled human capital and resource. There is currently a lack of robust postharvest research networks outside of the developed world, and insufficient global funding mechanisms which can support such interdisciplinary collaborations. There is thus a collective need for schemes which encourage inter-supply chain research, knowledge exchange and capacity building to reduce food losses and waste
Natural vitamin B12 and fucose supplementation of green smoothies with edible algae and related quality changes during their shelf life

Background. Some algae are an excellent sources of vitamin B12, of special interest for vegetarian/vegan consumers, and of fucose to supplement fruit and vegetable beverages such as smoothies. Nevertheless, supplementation of smoothies with algae may lead to possible quality changes during smoothie shelf life that need to be studied. Therefore, the quality changes in fresh green smoothies supplemented (2.2%) with nine edible algae (sea lettuce, kombu, wakame, thongweed, dulse, Irish moss, nori, Spirulina and Chlorella) were studied throughout 24 days at 5 °C. Results. The initial vitamin C content – 238.7–326.0 mg kg−1 fresh weight (FW) – of a 200 g portion of any of the smoothies ensured full coverage of its recommended daily intake, and still supplying 50–60% of the recommended intake after 7 days. Chlorella and Spirulina smoothies showed the highest vitamin B12 content (33.3 and 15.3 µg kg−1 FW, respectively), while brown algae showed fucose content of 141.1–571.3 mg kg−1 FW. These vitamin B12 and fucose contents were highly maintained during shelf life. Conclusion. The Spirulina supplementation of a 200 g smoothie portion ensured full coverage of the recommended vitamin B12 intake, with lower vitamin C degradation, during a shelf life of 17 days. Furthermore, thongweed and kombu are also considered as excellent fucose sources with similar shelf life.The authors are grateful to Spanish Ministry of Economy and Competitiveness, Project AGL2013−48830−C2−1−R and FEDER for financial support. We would also like to thank SAKATA SEED IBERICA S.L. for providing Bimi® samples
The production of hydrolysates from industrially defatted rice bran and its surface image changes during extraction

BACKGROUND\ud
This research employed mild-subcritical alkaline water extraction (SAW) technique to overcome the difficulty of active compounds extractability from an industrially defatted rice bran (IDRB). Mild-SAW (pH 9.5, 130 °C, 120 min) treatment, followed by enzymatic hydrolysis (Protease G6) were applied to produce rice bran hydrolysate (RBH). Response surface methodology was used to identify proteolysis conditions for maximizing protein content and ABTS radical scavenging activity (ABTS-RSA). The microstructural changes during the extraction occurring in the IDRB were monitored. The selected RBH was characterised for protein recovery, yield, antioxidant activities, phenolic profile and hydroxymethylfufural (HMF) content.\ud
\ud
RESULTS\ud
Optimal proteolysis conditions were at 20 mL kg-1 IDRB (E/S) for 6 h. Under these conditions, the yield, ABTS-RSA, Ferric reducing antioxidant power and the total phenolic content of the RBH were 46.1%, 294.22 μmol trolox g-1, 57.72 μmol FeSO4 g-1, and 22.73 mg gallic acid g-1, respectively, with relatively low HMF level (0.21 mg g-1). The protein recovery was 4.8 times greater than the recovery obtained by conventional alkaline extraction. Its major phenolic compounds were p-coumaric and ferulic acids. The microstructural changes of IDRB confirmed that the mild-SAW/Protease G6 process enhanced the release of active compounds.\ud
\ud
CONCLUSION\ud
The process of mild-SAW followed by proteolysis promotes the release of active compounds from IDRB
1-Methylcyclopropene (1-MCP) effects on natural disease resistance in stored sweetpotato

BACKGROUND
The potential of 1-methylcyclopropene (1-MCP) to maintain postharvest storage of sweetpotato was studied. In two separate experiments, the orange-fleshed sweetpotato cv. Covington was fumigated with 1-MCP (1.0 μL L-1, 24 h) and roots stored at 15 oC. During storage, samples were evaluated for the respiration rate, sprout growth, weight loss, incidence of decay and changes in dry matter. The roots were further assayed for the temporal changes in individual non-structural carbohydrates and phenolic compounds in the skin and flesh tissues of the proximal (stem end), middle, and distal (root end) regions.

RESULTS
1-MCP treatment reduced root weight loss and decay but respiration rate and non-structural carbohydrates were not affected. No sprouting was recorded irrespective of the treatment. 1-MCP transiently suppressed the accumulation of individual phenolic compounds, especially in the middle and distal segments. This accentuated the proximal dominance of phenolic compounds. Isochlorogenic acid A and chlorogenic acid were the dominant phenolics in the skin and flesh tissues, respectively.

CONCLUSION
1-MCP treatment may have anti-decay effect and reduce weight loss. Therefore, storage trials which involve the use of continuous ethylene supplementation to inhibit sprout growth may be combined with 1-MCP to alleviate ethylene-induced weight loss and decay in sweetpotato
Concentrates of triterpenic acids obtained from crude olive pomace oils: characterization and evaluation of their potential antioxidant activity

31 Páginas, 5 Tablas, 1 FiguraBackground\ud
\ud
Pentacyclic triterpenic acids (TA) are phytochemicals of increasing nutritional interest owing to their bioactive properties, such as anti‐inflammatory, antitumoral, antihyperglycemic and hepatoprotective. Crude olive pomace oils constitute a non‐exploited significant source of these compounds. In the present study, concentrates of TA were extracted and characterized from crude olive pomace oils that were obtained by centrifugation and subsequent solvent extraction, respectively. Specifically, the concentrates were obtained from the byproduct generated in the filtration of the oils. The solids were subjected to Soxhlet extractions with hexane to remove the residual oil and then with ethanol for the TA extraction.\ud
Results\ud
\ud
Concentrates containing 850–980 g kg−1 TA were isolated from the oils obtained by centrifugation, whereas those isolated from oils obtained by hexane extraction presented levels of TA that ranged from 510 to 900 g kg−1. Oleanolic (OA) and maslinic (MA) acids were the TA found in the concentrates. The relative contents of OA and MA were, respectively, 30:70 (w/w) and 77:23 (w/w). All concentrates also presented phenolic compounds at levels of g kg−1 and displayed slight antioxidant properties.\ud
Conclusion\ud
\ud
Concentrates of TA, containing MA and OA, can be readily obtained from a byproduct generated by filtration of crude olive pomace oils. Concentrates isolated from oils obtained by centrifugation were rich in MA, whereas those from oils extracted with hexane were rich in OA. The concentrates showed slight antioxidant properties that can be mainly attributed to the presence of phenolic compounds and not to TA. © 2018 Society of Chemical IndustryThis work was funded by the Spanish Ministry of Economy, Industry and Competitiveness through project AGL2013‐45110‐R. We would also like to thank ‘Oleícola El Tejar’ for providing the oils, Irene Pérez de la Rosa for technical assistance, Concepción Romero Barranco for the HPLC analyses of TA and phenolic compounds and José Julián Ríos Martín for the GC‐MS analysis.Peer reviewe
Reducing the cooking water-to-dried pasta ratio and environmental impact of pasta cooking

BACKGROUND: During daily pasta cooking, the general consumer pays little attention to water and energy issues. The aims of this work were to measure the cooking quality of a standard format of dry pasta by varying the water-to-dried pasta ratio (WPR) in the range of 12 to 2 L/kg, and assess the environmental impact of pasta cooking. 
RESULTS: In the above range of WPR, the cooked pasta water uptake (1.3±0.1 g/g), cooking loss (3.7±0.9 %), and degree of starch gelatinization (11.2±0.8 %) resulted to be about constant. Also, the main Texture Analysis parameters (e.g., cooked pasta hardness at 30 and 90% deformation, and resilience) showed no statistically significant sensitivity to WPR. On the contrary, by reducing WPR from 12 to 2 L/kg, the specific electric energy consumption linearly decreased from 1.93 to 0.39 Wh/g, and carbon footprint and eutrophication potential of pasta cooking lessened by about 80% and 50%, respectively. 
CONCLUSIONS: By using the environmentally sustainable pasta cooking procedure tested here, there would be no need to cook dry pasta in a large excess of water (i.e., 10 L/kg), as commonly suggested by the great majority of pasta manufacturers. However, such a great mitigation in the environmental impact of pasta cooking asks for novel more proper pasta cookers than those currently used
Effect of separate pod and root zone temperatures on yield and seed composition of three Spanish cultivars of groundnut (Arachis hypogaea L)

The effect of separate pod and root temperature regimes (all four combinations of 28/22 and 40/34 °C day/night temperature), imposed from the time of peg penetration until harvest, on yield and seed composition of three Spanish genotypes of groundnut (Arachis hypogaea L) was investigated. A decrease in pod temperature from 40/34 to 28/22 °C increased yield and oil, starch and protein mass per plant irrespective of root temperature. Additionally, a reduction in pod temperature decreased protein concentration and increased the sum of oil and starch concentration at a root temperature of 28/22 °C, whereas at a root temperature of 40/34 °C a decrease in pod temperature increased protein concentration. Root temperature reduction diminished oil concentration of genotypes AH 6179 and TMV 2 at a pod temperature of 40/34 °C. A decline in pod temperature affected fatty acid composition through a decrease in palmitic acid irrespective of root temperature and an increase in linoleic acid at a root temperature of 28/22 °C. A root temperature effect on fatty acid composition was not detected. It is concluded that field management practices and choice of genotype can influence groundnut yield and seed composition through effects on pod and root temperatur