pinecone/core-2020-05-10-deduplication huggingface dataset:
A Comparative Ultrastructural Study of Chondrosarcoma, Chordoid Sarcoma, and Chordoma

A morphologic and electron microscopic study was made of two chordoid sarcomas.
      These lesions were compared with two classical chondrosarcomas and two chordomas.
      These chondrosarcoma cells showed many features common to chondrocytes, such as
      abundant RER, well-developed Golgi complexes, and microvillous cytoplasmatic
      membranes. The chordoid sarcomas bore a close morphologic resemblance to the
      chordomas but the ultrastructural features revealed a close relationship to the
      chondrosarcomas. The chordoid sarcoma and chondrosarcoma cells had scalloped
      cytoplasmatic membranes, variable amounts of glycogen, round or oval nuclei and
      microfibrils, collagen, and electron-dense granules in the ground substance. The 
      chordoma was characterized by the presence of stellate and physalipherous cells, 
      as well as many transitional cells, with varying nuclear morphology; dilated and 
      irregular RER in contact with mitochondria and morphologically varied vacuoles
      are the main features in the cytoplasm. This study suggests that chordoid sarcoma
      represents a variety of the chondrosarcoma rather than a form of chordoma. These 
      findings also support the suggestion of Weiss that chordoid sarcoma is an
      extraskeletal myxoid chondrosarcom
Chromosome 1 abnormalities in cervical carcinoma

Abnormalities of chromosome 1 have been reported in a number of solid tumors and hematologic malignancies, indicating that this is a frequent event in neoplasia. Here we report our observations on aberrations of chromosome 1 in malignancies of the uterine cervix. Tumor material obtained from 148 patients with invasive carcinoma of the cervix and two cases of carcinoma in situ (CIS) was analyzed on direct preparations by G-banding. The results showed abnormalities of chromosome 1 to be one of the most common karyotypic changes, with 95% of the patients showing rearrangements of this chromosome. These changes were never seen as the sole abnormality but were always found in association with other chromosomal aberrations. Numerical rearrangements were present in 54% of the cases, with losses of unaltered chromosome 1 predominating. Consistent marker chromosomes included deletions of chromosome 1 at bands q32, p34, q42, p32, and p22, isochromosomes of both the "p" and "q" arms and translocations, particularly on the long arm. Specific regions on both arms of chromosome 1 (1p11-p13 and 1q21-q32) were preferentially overrepresented in changes involving this chromosome. Certain breakpoints were nonrandomly involved in the structural changes, particularly band 1q32 breaks occurring at this site in 88 instances. The presence of chromosome 1 aberrations in the two cases of CIS suggests that rearrangements of this chromosome are not always a secondary change contributing to the progression of the cancer, but also may represent an early cytogenetic event as in neuroblastoma, some leukemias, and myeloproliferative disorders
Treatment of hepatic epithelioid hemangioendothelioma with liver transplantation

Ten patients received liver transplants for unresectable epithelioid hemangioendothelioma (EHE). At the time of transplantation, four patients had microscopic metastases to the hilar lymph nodes, and one of the four also had metastases to a rib. The fifth patient had metastases to the lung, pleura, and diaphragm. The remaining five patients were believed to be free of metastatic disease. Two of these five patients died of metastatic disease at 3 and 16 months, respectively, after transplantation. Interestingly, all five patients with metastatic involvement are currently alive 40.6 ± 22 months (mean ± standard error of mean [SEM]) after transplantation, although one of these patients currently has metastatic disease to the lungs and mediastinum. Thus, the projected 5‐year actuarial survival rate is 76%, with two patients at risk after the third year. In conclusion, liver transplantation is a reasonable procedure for bulky, otherwise unresectable, EHE even in the presence of metastatic disease. Copyright © 1988 American Cancer Societ
Prognostic significance of DNA cytometry in cutaneous malignant lymphomas.

The current classification of cutaneous malignant lymphomas (ML) into low-grade and high-grade lymphomas was found to be of limited reproducibility and permitted only a rough prediction about outcome. With this in mind, the relationship between nuclear DNA content and both prognosis and histologic grading according to the Kiel classification was evaluated on Feulgen-stained imprint specimens. In all, 49 cases of malignant non-Hodgkin's lymphoma, primary of the skin or with an involvement of the skin as one of the first symptoms, were studied using a computerized high-resolution image analysis system. The 2c deviation index (2cDI), which reflects the variation of the nuclear DNA values around the normal diploid peak, was found to be the best prognostically relevant criterion. Using the 2cDI, a significant discrimination (P less than 0.001 in the U test) between low-grade and high-grade ML was achieved. The prognostic benefit of the 2cDI was well documented by a significant inverse correlation between the 2cDI and the period of time until the patients progressed at least into one higher stage or died of lymphoma (r equals -0.63, P less than 0.05). In addition, the 2cDI enabled prognosis of the course of disease. In the group with low 2cDI values (2cDI, less than 0.5), no progression of the disease was observed after 1 year. In the groups presenting with a 2cDI between 0.5 and 1.0 and higher than 1.0, a progression was found in 57% and 64% of the cases studied, respectively. In conclusion, these measurements indicate that the determination of DNA distribution patterns in imprint specimens allows a precise and objective prognostic evaluation of cutaneous ML
Ascitic fluid analysis for the differentiation of malignancy related and nonmalignant ascites

The authors tried to differentiate malignancy-related from nonmalignant ascites with a sequence of sensitive followed by specific ascitic-fluid parameters. There were four results of this study. First, of nine parameters investigated in a first series of 48 patients, 28 with nonmalignant and 20 with malignancy-related ascites, ascitic-fluid cholesterol and fibronectin yielded the best negative predictive value of 92% each. Carcinoembryonic antigen (CEA) and cytologic examination both showed a positive predictive value of 100%. Second, combining cytologic examination (sensitivity, 70%) and CEA determination (sensitivity, 45%) increased the sensitivity to 80%. Third, cytologic findings were negative in all ascitic-fluid samples with a cholesterol concentration below the cutoff value of 45 mg/100 ml. Fourth, based on the results of the first series of 48 patients, the diagnostic sequence with cholesterol as a sensitive parameter, followed by the combination of cytologic examination and CEA determination as specific parameters, was tested in a second series of 71 patients, 37 with nonmalignant and 34 with malignancy-related ascites. Again cytologic examination was negative in all samples with cholesterol levels below 45 mg/100 ml. In the total of 119 patients, this diagnostic sequence did not identify 9% of patients with malignancy-related ascites, and 82% of samples classified as malignancy related by cholesterol levels above 45 mg/100 ml were confirmed by positive cytologic examination and/or CEA level above 2.5 ng/ml. Thus, a diagnostic sequence with ascitic-fluid cholesterol determination, followed by cytologic examination and CEA determination, in samples with cholesterol levels above 45 mg/100 ml should permit a cost-efficient routine differentiation of malignancy-related from nonmalignant ascites.
Accelerated growth rates of recurrent hepatocellular carcinoma after liver transplantation

The growth rates of recurrent hepatocellular carcinoma (HCC) after orthotopic liver transplantation (OLTX) were estimated by calculating the tumor doubling time (TDT) in 20 patients. The mean TDT, calculated by multiple measurement of tumor size, was 44.3 ± 11.3 days (mean ± standard error) in 12 patients with pulmonary metastasis (range, 10 to 161 days) and 37.6 ± 8.9 days (range, 7 to 65 days) in 5 patients with liver allograft recurrence. The TDT as estimated by serum alpha‐fetoprotein (AFP) levels in 6 patients was 37.3 ± 10.0 days (range, 12 to 84 days). The mean TDT obtained from 5 control subjects with HCC who were treated with liver resection (without immunosuppression) was 273.8 ± 79.1 days (range, 82 to 560 days). The disease‐free period and survival time after OLTX both correlated well with the TDT (r = 0.546 and r = 0.701, respectively). The patients with fibrolamellar HCC had a greater TDT and a longer survival time than those with nonfibrolamellar HCC. Despite a wide range of TDT in patients who received transplants, their recurrent HCC tumors grew significantly faster than those of patients with the same disease who did not receive transplants. The factors involved in this accelerated growth rate may include the use of immunosuppressive drugs and the consequent suppression of host immunity against the growth of micrometastasis. Cancer 68:2095–2100. Copyright © 1991 American Cancer Societ
Quality of life changes during conformal radiation therapy for prostate carcinoma

BACKGROUND: \ud
\ud
The objective of this study was to describe prospectively quality of life (QOL) before and after radiotherapy for patients with prostate carcinoma.\ud
\ud
METHODS: \ud
\ud
Forty-three patients with T1-T3 prostate carcinoma who underwent conformal external beam radiation therapy were randomized either to the complete European Organization for Research and Treatment of Cancer (EORTC) QOL questionnaire (EORTC QLQ-C30) or the Medical Outcomes Study Group Short Form Health Survey (SF-36) at baseline, at 3 weeks and 6 weeks after initial treatment, and at 6 weeks and 5 months after the completion of radiotherapy. The measures were self-reported patient QOL, and values are given as the mean +/- standard error of the mean. Changes in QOL are described from baseline to the end of treatment in both questionnaire groups. \ud
\ud
RESULTS: \ud
\ud
Emotional role functioning, as measured with the SF-36 questionnaire, significantly improved from 68.2 +/- 9.9 at baseline to 93.3 +/- 5.2 at the end of therapy (P = 0.02). The EORTC QLQ-C30 questionnaire revealed consistent values of emotional functioning during treatment (72.7 +/- 5.9 at baseline) but showed a significant improvement 6 weeks after therapy (89.0 +/- 4.4; P = 0.01). Role functioning deteriorated from 80.1 +/- 6.5 at baseline to 62.5 +/- 8.8 at the end of radiotherapy (P = 0.02). Symptoms of fatigue were shown to increase significantly from 26.9 +/- 6.0 at baseline to 37.7 +/- 7.6 at the end of therapy (P = 0.02). No significant changes in the other dimensions were observed in either questionnaire. \ud
\ud
CONCLUSIONS: \ud
\ud
After radiotherapy for prostate carcinoma, patients experience a temporary deterioration of fatigue and role functioning, as measured with the EORTC QLQ-C-30. Despite physical deterioration, the authors observed an improvement in emotional functioning scores with both questionnaires. This may have been due to psychological adaptation and coping
Biallelic inactivation of the APC gene is associated with hepatocellular carcinoma in familial adenomatous polyposis coli

BACKGROUND
Certain primary hepatic tumors have been associated with familial adenomatous polyposis (FAP), a condition caused by germline mutations of the adenomatous polyposis coli (APC) gene. However, a genetic association between FAP and hepatocellular carcinoma (HCC) has not been shown. This study tested the hypothesis that biallelic inactivation of the APC gene contributed to the development of HCC in a patient with FAP and a known germline mutation of the APC gene at codon 208, but no other risk factors for HCC.

METHODS
Total RNA and genomic DNA were isolated from the tumor, and in vitro synthesized protein assay and DNA sequencing analysis were used to screen for a somatic mutation in the APC gene.

RESULTS
A somatic one–base pair deletion at codon 568 was identified in the wild-type allele of the APC gene.

CONCLUSIONS
To the authors' knowledge, this study provides the first evidence that biallelic inactivation of the APC gene may contribute to the development of HCC in patients with FAP. Cancer 2001;92:332–9. © 2001 American Cancer Society.PublishedN/
Contribution of femoral and proximal sciatic nerve branches to the sensory innervation of hindlimb digits in the rat

The present study was performed to investigate the possibility of 'aberrant' innervation of the tips of the hindlimb digits in the rat, i.e., from other sources than the femoral and the main sciatic branches (tibial, peroneal, sural). Cutaneous injections of fluorescent tracers in the digits were combined with either selective nerve transections to restrict afferent routes followed by detection of labeled neurons in dorsal root ganglia (DRGs), or by a delayed application of a second tracer to afferent nerves under study to detect double labeled neurons in DRGs. The results show that the tips of the digits were represented in DRGs L3-6. The femoral nerve afferents from digits 1 and 2 projected primarily to DRG L3 and to a smaller extent to DRG L4. A small number of neurons from primarily medial digits 1 and 2, but also from lateral digits 3-5, were found to project to DRGs L4 and L5 via a proximal branch that leaves the sciatic nerve near the sciatic notch and runs distally in the posterior part of the thigh, here called the musculocutaneous nerve of the hindlimb. We also have some evidence indicating innervation of the tips of the digits from the posterior cutaneous nerve of the thigh. Aberrant innervation such as that described here might contribute to remaining and perhaps abnormal sensibility after nerve injury and is of interest for the interpretation of results in experimental studies of collateral and regenerative sprouting after such injur
Broca's region subserves imagery of motion : a combined cytoarchitectonic and fMRI study

Broca's region in the dominant cerebral hemisphere is known to mediate the production of language but also contributes to comprehension. Here, we report the differential participation of Broca's region in imagery of motion in humans. Healthy volunteers were studied with functional magnetic resonance imaging (fMRI) while they imagined movement trajectories following different instructions. Imagery of right-hand finger movements induced a cortical activation pattern including dorsal and ventral portions of the premotor cortex, frontal medial wall areas, and cortical areas lining the intraparietal sulcus in both cerebral hemispheres. Imagery of movement observation and of a moving target specifically activated the opercular portion of the inferior frontal cortex. A left-hemispheric dominance was found for egocentric movements and a right-hemispheric dominance for movement characteristics in space. To precisely localize these inferior frontal activations, the fMRI data were coregistered with cytoarchitectonic maps of Broca's areas 44 and 45 in a common reference space. It was found that the activation areas in the opercular portion of the inferior frontal cortex were localized to area 44 of Broca's region. These activations of area 44 can be interpreted to possibly demonstrate the location of the human analogue to the so-called mirror neurones found in inferior frontal cortex of nonhuman primates. We suggest that area 44 mediates higher-order forelimb movement control resembling the neuronal mechanisms subserving speech. Hum. Brain Mapping 11:273-285, 2000. (C) 2000 Wiley-Liss, Inc
A point interpolation method for two-dimensional solids

A Point Interpolation Method (PIM) is presented for stress analysis for two-dimensional solids. In the PIM, the problem domain is represented by properly scattered points. A technique is proposed to construct polynomial interpolants with delta function property based only on a group of arbitrarily distributed points. The PIM equations are then derived using variational principles. In the PIM, the essential boundary conditions can be implemented with ease as in the conventional Finite Element Methods. The present PIM has been coded in FORTRAN. The validity and efficiency of the present PIM formulation are demonstrated through example problems. It is found that the present PIM is very easy to implement, and very flexible for obtained displacements and stresses of desired accuracy in solids. As the elements are not used for meshing the problem domain, the present PIM opens new avenue to develop adaptive analysis codes for stress analysis in solids and structures
Dynamic inflation of non-linear elastic and viscoelastic rubberlike membranes

International audienceThe present paper deals with the dynamic inflation of rubber-like membranes. The material is assumed to obey the hyperelastic Mooney's model or the non-linear viscoelastic Christensen's model. The governing equations of free inflation are solved by a total Lagrangian finite element method for the spatial discretization and an explicit finite-difference algorithm for the time-integration scheme. The numerical implementation of constitutive equations is highlighted and the special case of integral viscoelastic models is examined in detail. The external force consists in a gas flow rate, which is more realistic than a pressure time history. Then, an original method is used to calculate the pressure evolution inside the bubble depending on the deformation state. Our numerical procedure is illustrated through different examples and compared with both analytical and experimental results. These comparisons yield good agreement and show the ability of our approach to simulate both stable and unstable large strain inflations of rubber-like membranes
Disease mapping models: an empirical evaluation. Disease Mapping Collaborative Group

The analysis of small area disease incidence has now developed to a degree where many methods have been proposed. However, there are few studies of the relative merits of the methods available. While many Bayesian models have been examined with respect to prior sensitivity, it is clear that wider comparisons of methods are largely missing from the literature. In this paper we present some preliminary results concerning the goodness-of-fit of a variety of disease mapping methods to simulated data for disease incidence derived from a range of models. These simulated models cover simple risk gradients to more complex true risk structures, including spatial correlation. The main general results presented here show that the gamma-Poisson exchangeable model and the Besag, York and Mollie (BYM) model are most robust across a range of diverse models. Mixture models are less robust. Non-parametric smoothing methods perform badly in general. Linear Bayes methods display behaviour similar to that of the gamma-Poisson methods.status: publishe
Bayesian Modelling of Inseparable Space-Time Variation in Disease Risk

This paper proposes a unified framework for a Bayesian analysis of incidence or mortality data in space and time. We introduce four different types of prior distributions for space $\times$ time interaction in extension of a model with only main effects.  Each type implies a certain degree of prior dependence for the interaction parameters, and corresponds to the product of one of the two spatial with one of the two temporal main effects.  The methodology is illustrated by an analysis of Ohio lung cancer data 1968-88 via Markov chain Monte Carlo simulation. We compare the fit and the complexity of several models with different types of interaction by means of quantities related to the posterior deviance.  Our results confirm an epidemiological hypothesis about the temporal development of the association between urbanization and risk factors for cancer.
Modelling categorical covariates in Bayesian disease mapping by partition structures

We consider the problem of mapping the risk from a disease using a series of regional counts of observed and expected cases, and information on potential risk factors.  To analyse this problem from a Bayesian viewpoint we propose a methodology, which extends a spatial partition model by including categorical covariate information. Such an extension allows to detect clusters in the residual variation, reflecting further, possibly unobserved, covariates.  The methodology is implemented by means of reversible jump Markov chain Monte Carlo sampling.  An application is presented, in order to illustrate and compare our proposed extensions with a purely spatial partition model. Here we analyse a well-known dataset on lip cancer incidence in Scotland.
Familial Associations of Lipid Profiles: A Generalised Estimating Equations Approach

Elevated plasma levels of apolipoproteins A1 (apoA1) and B (apoB) are important protective factors and risk factors, respectively, for atherosclerosis and coronary heart disease. It is well known that both apoA1 and apoB reveal strong familial aggregation. Our goal was to investigate whether exogenous variables influence these associations. We used marginal regression models for the mean and association structure (Generalised Estimating Equations 2; GEE2) to analyse data from 1435 family members within 469 families of different sizes included in the Donolo-Tel Aviv Three-Generation Offspring Study. The usual robust variance matrix was approximated by extensions of jackknife estimators of variance to GEE2 models. Upon use of this approach estimation of standard errors in models with quite complex correlation structures was possible. All analyses were easily carried out using a menu-driven stand-alone software tool for marginal regression modelling. We demonstrate that a variety of hypotheses can be tested using Wald statistics by modelling regression matrices for the association structure. We show that correlation for apoB between parent-offspring pairs increased with decreasing age difference and that pairs with individuals of the same gender had more similar apoA1 levels than individuals of different gender. Associations between different relative pairs did not all agree with those expected from differences in kinship coefficients. The analysis using GEE2 models revealed structures that would not have been detected by other models and should therefore be used in addition to traditional approaches of analysing family data. GEE2 should be considered a standard method for the investigation of familial aggregation. [ Published in: Statistics in Medicine 19, Issue 24, 3345-3357 ]
Effect of airflow rate on yields of Steinernema carpocapse Az 20 in liquid culture in an external-loop airlift bioreactor

Maximization of the contact between males
and females is a key factor in the production of the nematode Steinernema carpocapsae in a bioreactor.
The influence of the airflow rate in male and female distribution and mass production in an external-loop bioreactor with a deceleration zone was studied. When operating
at an airflow rate of 0.05 vvm, a high retention of females in the deceleration zone of the bioreactor was observed and a larger nematode productivity was obtained.
At this aeration rate there was a higher proportion of males in that zone, which together with the lower
circulation rate, increases the probability of encounters, thereby explaining the increase in productivity