pinecone/core-2020-05-10-deduplication huggingface dataset:
Xeno-free cGMP isolation and cryopreservation of clinical grade stromal vascular fraction

Introduction In the last decades, adipose tissue (AT) has attracted attention of the scientific and industrial communities for its high therapeutic potential. When focusing on the clinical translation of such type of cells, aspects such as Good Manufacturing Practices (GMP) compliance, scalability, reproducibility, and protocol validation must be ensured. Manufacturing of cells must warrant high quality concerning cell viability, cell yield immunophenotype, and absence of any microbial or pathological contamination. This project focused on the development of a complete manufacturing and cryopreservation process of therapeutic cells for human clinical delivery, ensuring all parameters and characteristics listed above.

Materials and Methods The new protocol developed is particularly compact and ensures economies of scale when increasing the volume of processed AT, which reduces manufacturing cost. In this project 100-200g AT/human donor, obtained from liposuction or lipectomy procedures (n=15) were processed under GMP conditions. Stromal vascular fraction (SVF) isolation and cryopreservation was performed using xeno-free and GMP grade reagents. Cell number and viability, before and after cryopreservation, was determined by trypan blue exclusion dye by three operators. Immunophenotype of SVF subpopulations was evaluated by flow cytometry (FACSCanto and FACSDiva software) for the expression of mesenchymal, endothelial, hematopoietic and pericytic markers: CD31, CD34, CD45, CD73, CD90, CD105, CD146 and HLA-DR. Mesenchymal trilineage differentiation was performed to demonstrate potential of the isolated cells.

Results The developed xeno-free cGMP procedure for isolation of clinical grade SVF cells resulted in a cell yield of 2.4x105 ±0.9 cells/g AT, and cell viability of 85.4%±7.1, which demonstrated differentiation into mesodermal lineages. After xeno-free cryopreservation cell viability was 80.0%±10.1. Regarding immunophenotype of SVF subpopulations (Fig.1), when considering mesenchymal markers co-expressed with CD341, 12%±5 of the cells presented the CD90+CD34+CD73+CD105+ phenotype, while 1.3%±0.8 of cells expressed concomitant mesenchymal markers in the absence of CD34. Regarding endothelial markers, 15%±6 of the cells are CD45-CD34+CD31+. Preliminary analysis of samples demonstrated no significant difference of phenotypic expression before and after xeno-free cryopreservation.  

Discussion and Conclusions To the best of our knowledge this project is pioneer in providing clinical grade cryopreserved hSVF, ensuring extremely high viability post-thaw, and maintenance of immunophenotypic characteristics of its sub-populations. This achievement is of great relevance for therapeutic use of adipose tissue regenerative cells.

 References 1 Scherberich A. et al. World J Stem Cells 5, 1, 2013; 2 Bourin P. et al. Cytotherapy. 0, 1, 2013
Constraints on non-Standard Model Higgs boson interactions in an effective Lagrangian using differential cross sections measured in the H→γγ decay channel at √s=8TeV with the ATLAS detector

The strength and tensor structure of the Higgs boson’s interactions are investigated using an effective Lagrangian, which introduces additional CP-even and CP-odd interactions that lead to changes in the kinematic properties of the Higgs boson and associated jet spectra with respect to the Standard Model. The parameters of the effective Lagrangian are probed using a fit to five differential cross sections previously measured by the ATLAS experiment in the H→γγ decay channel with an integrated luminosity of 20.3fb−1at √s=8TeV. Inorder to perform a simultaneous fit to the five distributions, the statistical correlations between them are determined by re-analysing the H→γγcandidate events in the proton–proton collision data. No significant deviations from the Standard Model predictions are observed and limits on the effective Lagrangian parameters are derived. The statistical correlations are made publicly available to allow for future analysis of theories with non-Standard Model interactions
Factorization and regularization by dimensional reduction.

Since an old observation by Beenakker et al., the evaluation of QCD processes in dimensional reduction has repeatedly led to terms that seem to violate the QCD factorization theorem. We reconsider the example of the process  and show that the factorization problem can be completely resolved. A natural interpretation of the seemingly non-factorizing terms is found, and they are rewritten in a systematic and factorized form. The key to the solution is that the D- and (4−D)-dimensional parts of the 4-dimensional gluon have to be regarded as independent partons. \u
Exploring anomalous $HZ\gamma $ couplings in $\gamma $-proton collisions
  at the LHC

The $HZ\gamma $ coupling, which is highly sensitive to the new physics beyond
the standard model, is studied through the process $pp\rightarrow p\gamma
p\rightarrow pHX$ at the LHC. To this purpose, an effective Lagrangian, in a
model independent approach, with dimension six operators is considered in this
paper. New interaction terms regarding beyond the standard model physics
include the Higgs boson anomalous vertices in both CP-even and CP-odd
structures. A detailed numerical analysis is performed to scrutinize the
accurate constraints on the effective $HZ\gamma $ couplings and to discuss how
far the corresponding bounds can be improved. This is achieved by testing all
the efficient Higgs decay channels and increasing the integrated luminosity at
three different forward detector acceptance regions. The numerical results
propose that the Higgs photoproduction at the LHC, as a complementary channel,
has a great potential of exploring the $HZ\gamma $ couplings.Comment: 16 pages, 5 figures, 5 tables, version accepted for publication in
  PL
Search for charged Higgs bosons produced in association with a top quark and decaying via H±→τν using pp collision data recorded at √s=13 TeV by the ATLAS detector

Charged Higgs bosons produced in association with a single top quark and decaying via H±→τν are searched for with the ATLAS experiment at the LHC, using proton–proton collision data at √s=13 TeV corresponding to an integrated luminosity of 3.2 fb−13.2 fb−1. The final state is characterised by the presence of a hadronic τ   decay and missing transverse momentum, as well as a hadronically decaying top quark, resulting in the absence of high-transverse-momentum electrons and muons. The data are found to be consistent with the expected background from Standard Model processes. A statistical analysis leads to 95% confidence-level upper limits on the production cross section times branching fraction, σ(pp→[b]tH±)×BR(H±→τν), between 1.9 pb and 15 fb, for charged Higgs boson masses ranging from 200 to 2000 GeV. The exclusion limits for this search surpass those obtained with the proton–proton collision data recorded at √s=8TeV
Phenolic compounds and aroma-impact odorants in herb liqueurs elaborated by maceration of aromatic and medicinal plants in grape marc distillates

Twenty-eight commercial herb liqueurs, elaborated by maceration of aromatic and medicinal plants in grape marc distillate, were analysed. Thirty-two volatile compounds were identified and quantified by gas chromatographymass spectrometry, whereas seven phenols were determined by high-performance liquid chromatography with UV detection. All compounds showed significant differences among the samples analysed as consequence of the initial composition of the distillate and the plants and spices used in the liqueur elaboration. Of the 32 volatile compounds identified, 18 were considered to be impact odorants (odour activity value ?1) and these were classified into six odorant series. Spice, fruity and floral were the series that contributed the most to the aroma profile of the evaluated liqueurs. A first principal components analysis (PCA) was performed on the concentration of the seven phenols and these accounted for 75.37% of the total variance and a second PCA was performed on the concentration of the 18 impact odorants and these accounted for 60.96% of the total variance; allowing for the classification of the liqueur samples into three different groups.We are grateful to the Spanish Ministry of Economy and Competitiveness for the financial support of this work (project CTQ201571436-C2-1-R), which has partial financial support from the FEDER funds of the European Union. José Manuel Salgado was supported by the grant SFRH/BPD/84440/2012 from Fundação para a Ciência e Tecnologia - FCT, Portugal
Prediction and warning system of SEP events and solar flares 4 for risk estimation in space launch operations

YesA web-based prototype system for predicting solar energetic particle (SEP) events and solar flares for use by space launch operators is presented. The system has been developed as a result of the European Space Agency (ESA) project SEPsFLAREs (Solar Events Prediction system For space LAunch Risk Estimation). The system consists of several modules covering the prediction of solar flares and early SEP Warnings (labeled Warning tool), the prediction of SEP event occurrence and onset, and the prediction of SEP event peak and duration. In addition, the system acquires data for solar flare nowcasting from Global Navigation Satellite Systems (GNSS)-based techniques (GNSS Solar Flare Detector, GSFLAD and the Sunlit Ionosphere Sudden Total Electron Content Enhancement Detector, SISTED) as additional independent products that may also prove useful for space launch operators.This work has been developed in the frame of 34 SEPsFLAREs project (ESA Contract Number 4000109626/13/NL/ 35 AK), which is an activity funded by ESA/ESTEC Space Environ- 36 ment (TEC-EES) section. The authors of this work are grateful to 37 ESA’s MONITOR project (Contract Number 4000100988/2010/F/ 38 WE) for allowing the use of GSFLAD and SISTED products. 39 We also thank AGAUR (Generalitat de Catalunya) for the financial 40 support from Grant PDJ 2014 00074
Supersymmetric perturbations of the M5 brane.

We study long-wavelength supersymmetric deformations of brane solutions in supergravity using an extension of previous ideas within the general scheme of the blackfold approach. As a concrete example, we consider long-wavelength perturbations of the planar M2-M5 bound state solution in eleven-dimensional supergravity. We propose a specific ansatz for the first order deformation of the supergravity fields and explore how this deformation perturbs the Killing spinor equations. We find that a special part of these equations gives a projection equation on the Killing spinors that has the same structure as the κ-symmetry condition of the abelian M5 brane theory. Requiring a match between supergravity and gauge theory implies a specific non-linear gauge-gravity map between the bosonic fields of the abelian M5 brane theory and the gravity-induced fluid-like degrees of freedom of the blackfold equations that control the perturbative gravity solution. This observation sheds new light on the SUGRA/DBI correspondence
Eroding dipoles and vorticity growth for Euler flows in R 3 : Axisymmetric flow without swirl

A review of analyses based upon anti-parallel vortex structures suggests that structurally stable
dipoles with eroding circulation may offer a path to the study of vorticity growth in solutions of
Euler’s equations in R3
. We examine here the possible formation of such a structure in axisymmetric
flow without swirl, leading to maximal growth of vorticity as t
4/3
. Our study suggests that the
optimizing flow giving the t
4/3 growth mimics an exact solution of Euler’s equations representing
an eroding toroidal vortex dipole which locally conserves kinetic energy. The dipole cross-section
is a perturbation of the classical Sadovskii dipole having piecewise constant vorticity, which
breaks the symmetry of closed streamlines. The structure of this perturbed Sadovskii dipole is
analyzed asymptotically at large times, and its predicted properties are verified numerically. We
also show numerically that if mirror symmetry of the dipole is not imposed but axial symmetry
maintained, an instability leads to breakup into smaller vortical structures
Pseudorapidity dependence of the anisotropic flow of charged particles in Pb–Pb collisions at √sNN = 2.76 TeV

We present measurements of the elliptic (v2), triangular (v3) and quadrangular (v4) anisotropic azimuthal flow over a wide range of pseudorapidities (−3.5<η<5). The measurements are performed with Pb–Pb collisions at √sNN = 2.76 TeV using the ALICE detector at the Large Hadron Collider (LHC). The flow harmonics are obtained using two- and four-particle correlations from nine different centrality intervals covering central to peripheral collisions. We find that the shape of vn(η)\ud
 is largely independent of centrality for the flow harmonics n=2–4, however the higher harmonics fall off more steeply with increasing |η|. We assess the validity of extended longitudinal scaling of v2 by comparing to lower energy measurements, and find that the higher harmonic flow coefficients are proportional to the charged particle densities at larger pseudorapidities. Finally, we compare our measurements to both hydrodynamical and transport models, and find they both have challenges when it comes to describing our data
Search for top squark pair production in final states with one isolated lepton, jets, and missing transverse momentum in √s = 8 TeV pp collisions with the ATLAS detector

The results of a search for top squark (stop) pair production in final states with one isolated lepton, jets, and missing transverse momentum are reported. The analysis is performed with proton-proton collision data at √s = 8 TeV collected with the ATLAS detector at the LHC in 2012 corresponding to an integrated luminosity of 20 fb[superscript −1]. The lightest supersymmetric particle (LSP) is taken to be the lightest neutralino which only interacts weakly and is assumed to be stable. The stop decay modes considered are those to a top quark and the LSP as well as to a bottom quark and the lightest chargino, where the chargino decays to the LSP by emitting a W boson. A wide range of scenarios with different mass splittings between the stop, the lightest neutralino and the lightest chargino are considered, including cases where the W bosons or the top quarks are off-shell. Decay modes involving the heavier charginos and neutralinos are addressed using a set of phenomenological models of supersymmetry. No significant excess over the Standard Model prediction is observed. A stop with a mass between 210 and 640 GeV decaying directly to a top quark and a massless LSP is excluded at 95% confidence level, and in models where the mass of the lightest chargino is twice that of the LSP, stops are excluded at 95% confidence level up to a mass of 500 GeV for an LSP mass in the range of 100 to 150 GeV. Stringent exclusion limits are also derived for all other stop decay modes considered, and model-independent upper limits are set on the visible cross-section for processes beyond the Standard Model.European Organization for Nuclear ResearchUnited States. Dept. of EnergyNational Science Foundation (U.S.)Brookhaven National Laborator
Measurement of the W charge asymmetry in the W →μν decay mode in pp collisions at √s = 7 TeV with the ATLAS detector

This Letter reports a measurement of the muon charge asymmetry from W bosons produced in proton–proton collisions at a centre-of-mass energy of 7 TeV with the ATLAS experiment at the LHC. The asymmetry is measured in the W→μν decay mode as a function of the muon pseudorapidity using a data sample corresponding to a total integrated luminosity of 31 pb[superscript −1]. The results are compared to predictions based on next-to-leading order calculations with various parton distribution functions. This measurement provides information on the u and d quark momentum fractions in the proton.European Organization for Nuclear ResearchUnited States. Dept. of EnergyNational Science Foundation (U.S.)Brookhaven National Laborator
Measurement of J/ψ production in pp collisions at s√=2.76TeV

The production of J/ψ mesons is studied with the LHCb detector using data from pp collisions at  s√=2.76s=2.76  TeV corresponding to an integrated luminosity of 71 nb[superscript −1]. The differential cross-section for inclusive J/ψ production is measured as a function of its transverse momentum pT. The cross-section in the fiducial region 0 < pT  < 12 GeV/c and rapidity 2.0 < yy < 4.5 is measured to be 5.6 ± 0.1 (stat) ± 0.4 (syst) μb, with the assumption of unpolarised J/ψ production. The fraction of J/ψ production from b-hadron decays is measured to be (7.1 ± 0.6 (stat) ± 0.7 (syst))%.National Science Foundation (U.S.
Measurement of spin correlations in $t\overline{t}$ production using the matrix element method in the muon+jets final state in pp collisions at $\sqrt{s}$ = 8 TeV

The consistency of the spin correlation strength in top quark pair production with the standard model (SM) prediction is tested in the muon+jets final state. The events are selected from pp collisions, collected by the CMS detector, at a centre-of-mass energy of 8 TeV, corresponding to an integrated luminosity of 19.7fb$^{−1}$. The data are compared with the expectation for the spin correlation predicted by the SM and with the expectation of no correlation. Using a template fit method, the fraction of events that show SM spin correlations is measured to be 0.72 ± 0.08 (stat)$^{+0.15} _{−0.13}$(syst), representing the most precise measurement of this quantity in the muon+jets final state to date
Search for Higgs boson decays to a photon and a Z boson in pp collisions at root s = 7 and 8 TeV with the ATLAS detector

A search is reported for a neutral Higgs boson in the decay channel H→ZγH→Zγ, Z→ℓ+ℓ−Z→ℓ+ℓ− (ℓ=e,μℓ=e,μ), using 4.5 fb−1 of pp collisions at View the MathML sources=7 TeV and 20.3 fb−1 of pp collisions at View the MathML sources=8 TeV, recorded by the ATLAS detector at the CERN Large Hadron Collider. The observed distribution of the invariant mass of the three final-state particles, mℓℓγmℓℓγ, is consistent with the Standard Model hypothesis in the investigated mass range of 120–150 GeV. For a Higgs boson with a mass of 125.5 GeV, the observed upper limit at the 95% confidence level is 11 times the Standard Model expectation. Upper limits are set on the cross section times branching ratio of a neutral Higgs boson with mass in the range 120–150 GeV between 0.13 and 0.5 pb for View the MathML sources=8 TeV at 95% confidence level.G. Aad … P. Jackson … L. Lee … A. Petridis … N. Soni ... M. White ... et al. (The ATLAS Collaboration
A socioemotional wealth perspective on how collaboration intensity, trust, and international market knowledge affect family firms' multinationality

This paper was accepted for publication in the journal Journal of World Business and the definitive published version is available at http://dx.doi.org/10.1016/j.jwb.2016.02.004.Internationalization theory does not account for the priority family firms place on socioemotional wealth (SEW). This can reshape how critical theoretical dimensions of collaboration intensity, network trust, and international market knowledge exert their effects. Bringing together the internationalization model of Johanson and Vahlne (2009) with SEW theory, our study of 334 German-speaking family firms reveals international market knowledge mediates the relationship between collaboration intensity and family firms' multinationality. High network trust positively moderates the relationship between collaboration intensity and the acquisition of international market knowledge. Our work expands the predictive ability of Johanson and Vahlne's (2009) important model
Tactical treatment with copper oxide wire particles and symptomatic levamisole treatment using the FAMACHA © system in indigenous goats in South Africa

Haemonchosis is considered to be the most economically important gastrointestinal disease of small
ruminants in the tropics and subtropics. However, chemical anthelmintics, which were the mainstay of
control, have been compromised by a high prevalence of resistance worldwide. Copper oxide wire
particles (COWP) have been shown to have anthelmintic effects, but few studies have examined their use
under field conditions. The use of COWP was therefore evaluated as a tactical anthelmintic treatment in
indigenous goats raised under communal farming conditions in Bergville, KwaZulu-Natal Province,
South Africa. At the beginning of the summer rainfall season (October 2007), the faecal egg counts of
172 female goats belonging to 15 farmers were determined and this sampling continued every four weeks
until the second week of January 2008. The goats within each of the 15 herds were ranked according to
their faecal egg counts for this week. The goats were sequentially paired off within each ranking starting
with those goats with the highest counts. One goat from each pair was randomly allocated to a treated or
control group. Two weeks later, a 4 g COWP bolus was randomly administered to each goat in the treated
group. Faecal egg counts were carried out on the goats two weeks following treatment, and the sampling
of the goats then proceeded every four weeks until October 2008. Except for the six-week period prior to
the administration of the COWP, the goats were examined according to the FAMACHA© system and
symptomatically treated with 12 mg/kg levamisole when anaemic. The percentage reduction in faecal egg
count due to the COWP treatment was 89.0%. Mean pre- and post-treatment faecal egg counts for the
COWP-treated group (n = 73) were 2347 eggs per gram of faeces (epg) and 264 epg, respectively. The
corresponding values for the untreated controls (n = 66) were 2652 epg and 2709 epg. The prevalence of
Haemonchus spp. larvae in pre- and post-treatment faecal cultures was 72% and 46%, respectively.
Symptomatic anthelmintic treatments in combination with mid-summer tactical treatments with COWP appear to be useful strategies for the control of Haemonchus contortus in indigenous goats in this farming
system and this approach could have application in other similar agro-ecological zones.This project was funded by The Wellcome Trust project number 075812/A/04/7, Onderstepoort  Veterinary Institute (OVI), South Africa and KwaZulu-Natal Department of Agriculture, Environmental Affairs and Rural Development (KZN DoAEA&RD), South Africa.www.elsevier.com/locate/vetparhb2013ab201
An explanation of one-loop induced h→μτ decay

AbstractWe discuss a possibility to explain the excess of h→μτ at one-loop level. We introduce three generations of vector-like lepton doublet L′ and two singlet scalars S1,2 which are odd under Z2, while all the standard model fields are even under this discrete symmetry. We show that S1 can be a good dark matter candidate. We show that we can explain the dark matter relic abundance, large part of the discrepancy of muon g−2 between experiments and the standard model predictions, as well as the h→μτ excess of ∼1%, while evading constraints from experiments of dark matter direct detection and charged lepton flavor violating processes. We also consider prospects of production of S2 at LHC with energy s=14 TeV
Minimal seesaw model with S4 flavor symmetry

AbstractWe discuss a neutrino mass model based on the S4 flavor symmetry within the minimal seesaw framework, in which only two right-handed neutrinos are introduced and transform as 2 under S4. Although the model contains less free parameters compared to the typical seesaw models, it provides a successful description of the observed neutrino parameters, and in particular, a nearly tri-bimaximal mixing pattern can be naturally accommodated. In addition, the heavy right-handed neutrino masses are found to be non-degenerate, while only the normal hierarchical mass spectrum is compatible with experiments for light neutrinos