Home Products Protein Purification Protein purification is fundamental to the study of protein function and involves a series of processes to express, enrich and purify a protein of interest from a complex mixture such as cell lysate. The fastest and most powerful method for this purpose is affinity purification. We offer a range of protein purification kits and reagents for purification of recombinant proteins using affinity tags such as HaloTag, GST- or His-tag. We also offer Magne® Protein G/A Beads, which provide an easy-to-use and rapid method for purification of polyclonal and monoclonal antibodies from serum, ascites fluid and cell culture medium. Learn about Protein Purification Shop Protein Purification products Protein Purification Product Groups Antibody Purification Rapid, simple and robot-friendly antibody purification for manual and automated purification needs. Protein Purification Kits HaloTag, GST and polyhistidine-based protein purification options. Top Protein Purification Products for Your Lab Magne® Protein G and Magne® Protein A Beads Magnetic affinity beads with Protein A or G for Ab purification from culture media, ascites and serum samples. G7471, G7472, G7473, G8781, G8782, G8783 High Capacity Magne® Streptavidin Beads Magnetic affinity beads for binding biotinylated antibodies and proteins from complex biological samples with low nonspecific binding. V7820, V7830 Magne® HaloTag® Beads Magnetic capture of HaloTag® fusion proteins for protein pull-downs and purification. Magnetics enable streamlined protein purification, eliminate multiple centrifugation steps. G7281, G7282 Need a product modified? Contact us to discuss custom and bulk formulations, custom packaging and automation options for protein purification products. Learn More Protein Purification Resources Webinar Webinar: Streamline Antibody Enrichment with Bead-Based Chemistries Resource On-Bead Antibody Conjugation with Magne® Protein A and G Beads Resource HaloTag® Technology for Protein Labeling and Analysis Protein Purification Basics Protein purification is a fundamental step for analyzing individual proteins and protein complexes. Escherichia coli remains the first choice of many researchers for producing recombinant proteins due to ease of use, rapid cell growth and low cost of culture. Proteins expressed in E. coli can be purified in relatively high quantities, but these proteins, especially eukaryotic proteins, may not exhibit proper protein activity or folding. Cultured mammalian cells offer an alternative option for producing properly folded and functional mammalian proteins with appropriate post-translational modifications. To simplify purification, affinity purification tags can be fused to a recombinant protein of interest. Common fusion tags are polypeptides, small proteins or enzymes added to the N- or C-terminus of a recombinant protein. The first step in purification of a recombinant protein is the preparation of the cell lysate or supernatant. For secreted proteins, minimal supernatant preparation is required, followed by selective binding, washing and elution of the purified protein. After purification the protein may be cleaved with a protease to remove the affinity tag. Related Protein Analysis Products and Resources Categories Mass Spectrometry Protein Expression Protein Interactions Protein Detection Guides and Selectors Protein Expression Protein Purification Let's find the product that meets your needs. Talk to a Scientist Manos UK Don't miss out! Stay notified of Promega events, products and news. Sign Up Need Assistance? Contact Promega Customer and Technical Support Local Contact +31 (0)71 532 42 44 Products & Resources Products Applications Custom Capabilities Resources About Promega Company Info ISO Certification Corporate Responsibility Press Releases Careers © 2021 Promega Corporation, an affiliate of Promega Benelux BV. All rights reserved. Legal and Trademarks Privacy Policy Terms and Conditions Trademarks Promotional Terms and Conditions Terms and Conditions of Sale and Services Manage Cookie Consent English – Belgium Promega's Cookie Policy Close We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy. If you are located in the EEA , the United Kingdom, or Switzerland, you can change your settings at any time by clicking Manage Cookie Consent in the footer of our website. Manage My Cookies Reject All Cookies Yes, I Accept