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Support Center Login Blog Contact Us Solutions PROPERTIES Molar Mass Size Charge & Zeta Potential Interactions Conformation Conjugation --- 2 (2) --- 2 TECHNIQUES SEC-MALS FFF-MALS CG-MALS DLS MP-PALS SEC-IV --- 2 (3) APPLICATIONS Biotherapeutics Proteins Biopolymers Synthetic Polymers Nanoparticles --- 2 (4) OUR CUSTOMERS User Profiles SECTORS SERVED Biopharmaceutical Chemical Medical Device Academic Government Products INSTRUMENTS Instrument Overview MALS DLS & ELS Refractometers Viscometers FFF CG-MALS Request Product Quote --- 4 ACCESSORIES Accessories Overview --- 3 SOFTWARE ASTRA DYNAMICS CALYPSO ECLIPSE Host Computer Requirements --- 5 SERVICES Customer Support On-Site Services Compliance Sample Analysis Service Request Contract Labs --- 6 Library REFERENCES Featured Publications Bibliography FFF Bibliography --- 7 APPLICATION NOTES Search App Notes By Technique By Application BLOGS Blogs --- 8 WEBINARS By Technique By Application By Language --- 9 THEORY Introduction MALS FFF CG-MALS DLS ELS --- 10 Support Support Center Login Support Registration Firmware Updates Current Instruments Previous Instruments Support Help Training & Events Light Scattering Univ. Training Dates ILSC ILSC 2015 Past Colloquia User Meetings DC Region Indy Mid-Atlantic Region Northeast Region Research Triangle SF Bay Area Southern CA Region Live Webinars Seminars Calendar Application Note Contest LSU Registration LSU Germany Registration Form About Us Team Wyatt Executives R&D Customer Service & Support Applications Regional Sales & Support Marketing Team Wyatt Regions Careers Our History Scientific Advisors Robert H. Grubbs, Ph.D Lin Randall, Ph.D Trademarks Museum Marketing Campaigns ISO Certification Consultants Dr. Stepan Podzimek Contact Wyatt US & Canada Wyatt Northeast Lab Wyatt China Wyatt Europe Wyatt Technology Europe - Additional Information Wyatt France Wyatt UK Social@Wyatt Global Offices Email Signup Multi-Angle and Dynamic Light Scattering: Essential tools for characterizing proteins, polymers, nanoparticles and more What Can I Measure? What Can I Analyze? Biotherapeutics Proteins Biopolymers Synthetic Polymers Nanoparticles Aggregation While SEC-MALS with a DAWN or miniDAWN is the primary means of characterizing soluble protein aggregates, FFF-MALS, CG-MALS and DLS are valuable orthogonal techniques to meet FDA requirements. Conjugation SEC or FFF plus triple detection UV-MALS-RI and ASTRA software determine the molar mass of protein and modifier in conjugated biotherapeutics such as antibody-drug conjugates or PEGylated proteins. Formulation The DynaPro Plate Reader II performs high-throughput screening of excipients and buffers for optimal formulation in situ in standard microwell plates, detecting aggregation as well as conformational and colloidal stability indicators. Interaction CG-MALS combines a Calypso II and DAWN or miniDAWN to characterize the affinity and absolute molecular stoichiometry of drug-target binding, without labeling or immobilization. It also analyzes self-association at low, moderate and high concentrations. Read more... Identification A DAWN or miniDAWN MALS detector, combined with a Wyatt SEC column and the Optilab T-rEX dRI detector, determine absolute molar masses and sizes of peptides, proteins and other biomacromolecules independently of column calibration. Purification Validate protein purification and stability quickly and easily with a DynaPro NanoStar cuvette-based, combined dynamic light scattering & static light scattering detector, or a high-throughput DynaPro Plate Reader II. Charge The Möbiuζ is the only electrophoretic light scattering detector capable of providing reliable and repeatable measurements of a protein's net charge in high-salt buffers. Binding CG-MALS combines a Calypso and DAWN or miniDAWN to characterize the affinity and absolute molecular stoichiometry of simple or complex biomolecular interactions. CG-MALS requires no labeling or immobilization, addressing self-association as well as hetero-association. Read more... Absolute Molar Mass and Size A DAWN MALS detector and Optilab T-rEX dRI detector work together to determine distributions of absolute molar mass and size of natural or synthetic polymers independently of column calibration. Biodegradation Light scattering is an excellent means for assessing the degradation of biopolymers as a result of exposure to heat, light, high or low pH, and other stimuli. Polysaccharides Key ingredients in cosmetics, the paper industry and food products, polysaccharides are also critical materials in biomedical applications such as vaccines and blood coagulants. Characterize all types of polysaccharides by SEC combined with Wyatt's on-line light scattering, dRI and viscosity detectors. Paper and Pulp Lignin and cellulose present some of the most challenging biopolymeric analytes due to their unique optical and morphological properties. Wyatt offers multiple solutions for meeting these challenges. Read more... Absolute Molar Mass and Size A DAWN MALS detector and Optilab T-rEX dRI detector work together to determine distributions of absolute molar mass and size of natural or synthetic polymers independently of column calibration. Branching and Conformation Incorporate a WyattQELS DLS module and/or a ViscoStar II differential viscometer to characterize the branching ratio and conformation of complex polymers. Field-Flow Fractionation The Eclipse DualTec provides true hydrodynamic fractionation without the stress, surface interactions and non-ideal separation present in SEC. Integrates perfectly with Wyatt MALS, dRI, DLS and viscometry detectors. dn/dc and Zimm plots Wyatt offers several options to simplify laborious, error-prone dn/dc and Zimm analyses for natural and synthetic polymers. Read more... Accurate Size Distributions Accurate size distributions with true ensemble representation require fractionation + independent, on-line determination of size. A DAWN HELEOS II / Eclipse FFF-MALS system can characterize simple or complex fluids incorporating macromolecules with large and small nanoparticles. Conformation and Composition Information about nanoparticle shape and structure can be obtained by combining MALS analysis with dynamic light scattering. The WyattQELS DLS module integrates into the DAWN or miniDAWN for simultaneous MALS and DLS analysis. Add a UV, fluorescence and/or Optilab T-rEX dRI detector for compositional studies. High-Throughput Sizing Need to characterize your nanoparticles under hundreds of solvent conditions in the space of a few hours? The DynaPro Plate Reader II measures size distributions by DLS, in situ in standard 96-, 384- or 1536-well microtiter plates. A camera conveniently photographs each well to identify precipitates. Zeta Potential / Charge The Möbiuζ is the only zeta potential detector capable of hands-free, high-throughput measurements by coupling to an autosampler. It measures charge and size simultaneously to monitor aggregation or degradation caused by the applied electric field. Read more... For essential macromolecular and nanoparticle characterization—The Solution is Light™ 13 Key Questions to Ask Get the inside scoop on buying a Multi-Angle Light Scattering (MALS) or Dynamic Light Scattering (DLS) instrument! Request the Ultimate Guide today. Get Ultimate Guide Need more information? Request Product Info Resources Email Signup Light Scattering University Webinars Bibliography Request Product Information Contact Us Support Login Careers Wyatt Technology is the recognized leader in light scattering instrumentation and software for determining the absolute molar mass, size, charge and interactions of macromolecules and nanoparticles in solution. Wyatt's line of multi-angle static light scattering products couple to size exclusion chromatography (SEC-MALS), field-flow fractionation (FFF-MALS), and stop-flow composition-gradient systems (CG-MALS). Our dynamic light scattering (DLS) products operate in traditional cuvette as well as on-line and automated, high-throughput modes. We also offer unique instruments for electrophoretic light scattering (MP-PALS), differential refractometry, and differential viscosity. +1 805-681-9009 Headquarters | 6330 Hollister Ave, Santa Barbara, CA 93117 | Global Offices Site Map | Feedback | Privacy Policy © Wyatt Technology Corporation. All rights reserved. Solutions PROPERTIES Molar Mass Size Charge & Zeta Potential Interactions Conformation Conjugation --- 2 (2) --- 2 TECHNIQUES SEC-MALS FFF-MALS CG-MALS DLS MP-PALS SEC-IV --- 2 (3) APPLICATIONS Biotherapeutics Proteins Biopolymers Synthetic Polymers Nanoparticles --- 2 (4) OUR CUSTOMERS User Profiles SECTORS SERVED Biopharmaceutical Chemical Medical Device Academic Government Products INSTRUMENTS Instrument Overview MALS DAWN HELEOS II DAWN 8+ miniDAWN TREOS II µDAWN DLS & ELS DynaPro Plate Reader II DynaPro NanoStar Möbiuζ DynaPro Plate Reader II for Biotherapeutic and Nanoparticle Development (2) DynaPro Plate Reader II for Biotherapeutic and Nanoparticle Development Refractometers Optilab T-rEX Optilab UT-rEX Viscometers ViscoStar III FFF Eclipse DualTec Eclipse AF4 CG-MALS Calypso II Request Product Quote --- 4 ACCESSORIES Accessories Overview MALS Accessories WyattQELS COMET SEC Columns WISH Injection Module DLS Compatibility Kit Batch Conversion Kit microCuvette Orbit DLS & ELS Accessories Quartz Cuvettes Disposable Cuvettes NanoFilter Kit Atlas Möbiuζ Dip Cell FFF Accessories Eclipse Channels Thermos Miscellaneous Accessories RI Calibration Kit Solaris SysTrack Microbatch Kit --- 3 SOFTWARE ASTRA DYNAMICS CALYPSO ECLIPSE Host Computer Requirements --- 5 SERVICES Customer Support On-Site Services Compliance Sample Analysis Service Request Contract Labs --- 6 Library REFERENCES Featured Publications Bibliography FFF Bibliography --- 7 APPLICATION NOTES Search App Notes By Technique SEC-MALS μSEC-MALS FFF-MALS CG-MALS Batch MALS DLS ELS SEC-IV By Application Biotherapeutics E-book Advancing Biopharma Analysis with a Light Scattering Toolkit Proteins Biopolymers Synthetic Polymers Nanoparticles BLOGS Blogs --- 8 WEBINARS By Technique SEC-MALS FFF-MALS CG-MALS SEC-IV DLS ELS By Application Biotherapeutics Proteins Polymers Nanoparticles By Language Register for Webinar --- 9 THEORY Introduction MALS Chromatography Mode Batch Mode FFF CG-MALS DLS ELS --- 10 Support Support Center Login Support Registration Firmware Updates Current Instruments Current Instruments HELEOS II TREOS II μDAWN Optilab (U)T-rEX ViscoStar III Eclipse DualTec/AF4/3+ Plate Reader II NanoStar Möbiuζ Previous Instruments Previous Instruments HELEOS I TREOS I Optilab rEX Eclipse 3 Plate Reader I ViscoStar Product Launch Presentations Support Help Training & Events Light Scattering Univ. Training Dates Training Dates - US Training Dates - Germany Training Dates – France Register for LSU France ILSC ILSC 2015 2015 Short Courses 2015 ILSC Photos Past Colloquia 2015 ILSC 2012 ILSC 2011 ILSC 2010 ILSC 2009 ILSC 2008 ILSC 2007 ILSC 2006 ILSC 2005 ILSC 2004 ILSC 2003 ILSC 2002 ILSC Focus Meetings 2015 Focus Meeting - Formulation 2012 Focus Meeting - DLS 2011 FFF Focus Meeting 2010 FFF Focus Meeting 2009 FFF Focus Meeting 2008 FFF Focus Meeting 2007 FFF Focus Meeting User Meetings DC Region DC Light Scattering User Meeting DC Regional Protein & Biotech Registration Indy Indy Light Scattering User Meeting Indy User Meeting Registration Mid-Atlantic Region Mid-Atlantic Polymer/Nanoparticle Mid-Atlantic Protein/Biotech Mid-Atlantic Protein & Biotech Registration test form Northeast Region Northeast Protein/Biotech Northeast Protein & Biotech Registration Research Triangle Research Triangle Protein & Biotech Registration Research Triangle Protein & Biotech SF Bay Area SF Bay Area Protein/Biotech SF Bay Area User Meeting Registration Southern CA Region Southern CA Protein/Biotech Southern CA Protein & Biotech Registration Live Webinars Seminars Calendar Application Note Contest LSU Registration LSU Germany Registration Form About Us Team Wyatt Executives Philip J. Wyatt, Ph.D. Ross Bryant Geofrey K. Wyatt Clifford D. Wyatt Steve Minne R&D Craig Bennett, Ph.D. Vincent H. Hsieh, Ph.D. Michael I. Larkin, Ph.D. Steven P. Trainoff, Ph.D. Customer Service & Support Sigrid C. Kuebler, Ph.D. Applications Jeffrey A. Ahlgren, Ph.D. Michelle H. Chen, Ph.D. Sophia Kenrick, Ph.D. Regional Sales & Support John Champagne, Ph.D. Robert P. Collins Stephanie Cope, Ph.D. Andrew O. Meyer, Ph.D. Marketing Daniel Some, Ph.D. Team Wyatt Regions Team Wyatt US & Canada Team Wyatt Europe Christoph Johann Dr. Jacek Achrem-Achremowicz Christian Denisse Dr. Christian Ackerschott Jens R. Reichenberger Dr. Roger Scherres Dr. Dierk Roessner Milena Johann Sebastian Viertel Team Wyatt France & UK Nicolas Mignard Lionel Rouzic Stephanie Terme-Ferrari Careers Our History Scientific Advisors Robert H. Grubbs, Ph.D Lin Randall, Ph.D Trademarks Museum Marketing Campaigns ISO Certification Consultants Dr. Stepan Podzimek Contact Wyatt US & Canada Wyatt Northeast Lab Wyatt China Wyatt Europe Wyatt Technology Europe - Additional Information Wyatt France Wyatt UK Social@Wyatt Global Offices Email Signup