FIND A DISTRIBUTOR SALES@BIONET.COM CONTACT PRODUCTS SERVICES THE KNOWLEDGE HUB WHY BIONET? COMPARISON LEARN WITH BIONET THE KNOWLEDGE HUB Support is at the core of Bionet’s strategy, and this is a tangible example of it. Here you will find the resources that you need to push and enhance your bioprocess forward, as well as to feel part of its scientific community. CATEGORIES Expert References See all > Details that Matter See all > Scientific Academy See all > Nice Offers See all > DETAILS THAT MATTER BIONET: YOUR BIOPROCESS TECH AND SERVICE PARTNER FROM THE LAB TO YOUR INDUSTRIAL BIOPRODUCTION PLANT See more > SCIENTIFIC ACADEMY CHARACTERIZATION OF A BACILLUS AMYLOLIQUEFACIENS FERMENTATION FOR A FED-BATCH STRATEGY USING THE BIONET’S BBREATH EXHAUST GAS ANALYZER See more > EXPERT REFERENCES HOW TO USE THE MICROBIAL RESPIRATION IN THE AEROBIC FERMENTATION PROCESS: OUR, CER AND RQ DETERMINATION WITH BIONET’S BBREATH See more > SCIENTIFIC ACADEMY SCALE-UP OF E. COLI CULTURE FOR OPTIMAL FED-BATCH STRATEGY USING EXPONENTIAL FEEDING Escherichia coli is one of the most popular expression systems for the production of recombinant proteins. In the present study, a brief protocol to apply a fed-batch mode at lab scale with E.coli and the scale-up to 30 liters was performed. The high cell density of E.coli growth was achieved using a fed-batch mode in […] See more > SCIENTIFIC ACADEMY RECOMBINANT PROTEIN EXPRESSION IN PICHIA PASTORIS IN A BIOREACTOR VIA SEMI-CONTINUOUS MODE Pichia pastoris has been successfully used as an efficient expression system for heterologous protein production. Herein, we report the development of a semi-continuous process to improve the production of recombinant proteins in P. pastoris by its cultivation in a BIONET’s F1 benchtop 3-liter bioreactor equipped with a continuous process module (CPM).Six repetitive batches were conducted […] See more > DETAILS THAT MATTER ADDITION AND BIOREACTOR WEIGHT CONTROLS There are different fermentation modes in bioreactor systems: batch, fed-batch, and continuous mode. The batch mode is the simplest. The bioreactor is filled with the medium containing all the components that the organism requires to grow or produce determined biomolecules like a metabolite or protein. This fermentation mode has a production limitation: once the limiting […] See more > EXPERT REFERENCES TECHNOLOGY CHARACTERIZATION TEMPERATURE CONTROL part of the work at Bionet Pilot Plant is to test and characterize all our technologies as well as to find new process optimization strategies, wich are the aim of this series Request now > EXPERT REFERENCES A GUIDE ON MEMBRANE SELECTION The most common membrane modules used in the Biotech industry for Tangential Flow Filtration (TFF) are cassettes, hollow fiber and ceramic membranes. Each membrane type has certain characteristics (see Table 1) in terms of batch volume, sensibility to and with product type, and its own lifetime, that makes it more or less suitable for a […] See more > EXPERT REFERENCES CONTINUOUS PROCESS The Continuous Process in the context of a bioreactor operation mode is used to prolong the production phase of a culture during its growth in a steady-state (at a constant rate and in constant conditions) to achieve higher biomass and/or metabolites amounts. There are two main process control strategies that can be implemented in Continuous […] See more > EXPERT REFERENCES 5 STEPS INTO SCALE-UP OF MICROBIAL FERMENTATION Planning the jump into Industrial is a challenging experience that all successful bioprocesses and bioprocesists go through. With many factors having to be taken into account (at the biological, biochemical, mechanical levels among others), there is no other option than to follow a plan. What is the process information that cannot be dismissed? What process […] Request now > DETAILS THAT MATTER FLEXIBLE BENCHTOP BIOREACTORS See more > SCIENTIFIC ACADEMY EFFECT OF FERMENTATION DEFOAMING AGENTS ON TFF See more > SCIENTIFIC ACADEMY PRODUCTIVITY ENHANCEMENT OF B. SUBTILIS BY CASCADE CONTINUOUS FERMENTATION The majority of microbial fermentations are performed in batch or fedbatch mode, further increasing energy consumption and steps, limiting the productivity, conducting high costs. In some cases, these limitations are based on the equipment and technologies available [1]. The successful establishment of open production process based on continuous mode will lead to reducing the production […] See more > NICE OFFERS BLACK FRIDAY Find discounts on Sterile Addition Accessories during Bionet’s Technological Black Friday See more > SCIENTIFIC ACADEMY RECOMBINANT PROTEIN EXPRESSION IN P. PASTORIS IN A BIOREACTOR VIA A SEMICONTINUOUS MODE Pichia pastoris has been successfully used as an efficient expression system for heterologous protein production.Herein, we report the development of a semicontinuous process to improve the production of recombinant proteins in P.pastoris by its cultivation in a BIONET’ s F1 benchtop bioreactor of 3 L equipped with a continuous process module(CPM). Six repetitive batches were […] See more > NICE OFFERS BIOQUIZ See more > SCIENTIFIC ACADEMY MONOCLONAL ANTIBODY PRODUCTION The core of the technological infrastructure required for biopharmaceutical manufacturing is the bioreactor, used in all stages from R&D to biomanufacturing. The F0-BABY bioreactor line by BIONET is comprised of robust and user-friendly laboratory bioreactor models that can be configured for different cell expression systems like microbial cultures or cell cultures. In the present work, […] See more > EXPERT REFERENCES CHEMICAL CLEANING OF MEMBRANES The chemical cleaning of the ceramic and/or hollow fiber membranes after a filtration stage must receive the attention it deserves. Having defined an effective cleaning protocol is just as important as having optimized filtration conditions when dealing with a tangential flow filtration process in your downstream. Moreover, I would dare to say, the latter is […] See more > EXPERT REFERENCES NEVER GIVE ANYTHING FOR GRANTED WHEN IT COMES TO CULTIVATING MAMMALIAN CELLS! It is widely agreed that the most important quality factor of a bioreactor is its capacity to keep its inside sterile throughout the whole process and despite the number of operational human interactions that will take part during such period, including sampling and harvesting steps. Therefore, it is widely assumed that a bioreactor will do […] See more > DETAILS THAT MATTER CUSTOMIZATION OF AGITATION SYSTEMS AT ITS BEST At BIONET, practical integration of knowledge derived from research as well as that gained from our clients’ experience happens on a continuous manner. This is vital to our evolution as a company and to the technological development and success of our products. An example of this is the effort that we deploy in adapting the […] See more > EXPERT REFERENCES THE DILUTION RATE (D) AND THE CONTINUOUS FERMENTATION PROCESS When a continuous fermentation process is carried out, it is critical that the condition of steady-state must be reached. The main process parameters that can be used for the chemostat technique are the dilution rate, specific growth rate and the yield of the product on the substrate. The dilution rate (D), usually in units […] See more > GET MORE INFO I have read and accept the Legal Notice and the Privacy Policy. 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