Contact Us Manufacturing Engineered Adoptive Cell Therapies ACTengine® Patients eligible for clinical trials with ACTengine® product candidates have a portion of their white blood cells collected using a well-established process called leukapheresis, a procedure in which a fraction of the white blood cells of a patient is extracted from their peripheral blood. These white blood cells are transferred to a manufacturing facility where peripheral blood mononuclear cells (“PBMCs”), which are a subset of white blood cells, are isolated from the leukapheresis product. PBMCs or a selected subset of T cells (e.g. CD8+ T cells) is the starting point of the ACTengine® manufacturing process. T cells contained within PBMCs are activated and subsequently mixed with a lentiviral vector which introduces the genes encoding the target specific TCR into the T cells. These genetically engineered T cells are expanded in the presence of a cytokine mixture. All in all, the total ACTengine® manufacturing time is ~1 week. The ex vivo expanded T cells are concentrated and frozen before undergoing quality control release testing. The resulting cell product can be stored frozen long-term until the patient is ready to receive the infusion. Next-generation Enhancement Strategies Immatics’ latest proprietary ACTengine® manufacturing processes are designed to generate cell product candidates within a short manufacturing window and deliver highly proliferative T cells, with the capability to infiltrate the patient’s tumor and function in a challenging solid tumor microenvironment. Immatics is actively investigating multiple next-generation enhancement strategies to render T cells even more potent to combat solid tumors. For advanced-stage clinical trials and commercial supply, manufacturing processes are planned to be further optimized to ensure a robust manufacturing capability incorporating functionally closed and automated manufacturing systems as well as the use of serum free, chemically defined media. Immatics’ T cell products are manufactured by Immatics personnel at the UTHealth Evelyn H. Griffin Stem Cell Therapeutics Research Laboratory in a state-of-the-art cGMP facility exclusively used by Immatics in Houston, Texas. TCER® A TCER® consists of three distinct elements: Immatics’ TCER® molecules can be produced and purified utilizing established processes to manufacture antibodies. Immatics’ TCER® scaffold is the result of a campaign to engineer and evaluate various molecular scaffolds incorporating binding domains derived from affinity and stability enhanced TCRs and from T cell recruiting antibodies, respectively. The TCER® architecture, in Immatics’ in vitro testing, proved to be superior to other tested scaffolds with respect to preclinical efficacy, stability and physio-chemical properties, so called “developability”. TCER® molecules can readily be expressed in CHO-cells with titers comparable to antibody-based biologics. The TCER® protein can be purified using common chromatographic techniques and size-exclusion-chromatography, facilitating the cGMP-compliant manufacturing in established plants. The manufacturing development phase of IMA401 TCER® is ongoing and includes cell line development, upstream and downstream process development, GMP production, fill and finish, release testing, storage and stability testing. Immatics N.V. Immatics Biotechnologies GmbH Paul-Ehrlich-Str. 15 72076 Tuebingen Germany Phone: +49 7071 5397-0 Fax: +49 7071 5397-900 E-Mail: info@immatics.com Immatics US, Inc. 2201 W. Holcombe Blvd., Suite 205 Houston, Texas 77030 Business line: +1 346 204-5400 Fax line: +1 346 204-5391 E-Mail: info@immatics.com Immatics Biotechnologies GmbH Machtlfinger Str. 11 81379 Munich Germany Phone: +49 89 540415-0 Fax: +49 89 540415-905 E-Mail: info@immatics.com Imprint Privacy Policy English Privacy Policy German Privacy Settings © 2021 Immatics N.V. By clicking “Accept All Cookies”, you agree to the storing of cookies on your device to enhance site navigation, analyze site usage, and assist in our marketing efforts. Accept All Cookies Privacy Settings