Home Products Immunoassays Lumit™ Immunoassays for Research Lumit™ Immunoassays provide a simple and fast alternative to conventional immunoassay methods including sandwich ELISAs and Western blots. Lumit™ assays are sensitive, have broad dynamic range and can be completed in as little as 30 minutes, making them a compelling alternative to labor-intensive ELISA and Western blot methods. Advantages of Lumit™ Assays over conventional immunoassays include: Simple add-mix-read protocol with no washing steps Direct analyte measurement in the cell culture plate or on medium removed from the cells No immobilization to plates, beads or other surfaces required Sensitive luminescence detection with wide dynamic range Lumit™ Assays are available as catalog items, early-access materials or by custom order. Learn about Lumit™ Immunoassays Shop Lumit™ Immunoassays New bioluminescent assay to study SARS-CoV-2 neutralizing antibody response. Free webinar. How Lumit™ Immunoassays Work Lumit™ Immunoassays are based on NanoLuc® Binary Technology (NanoBiT®). In Lumit™ Immunoassays, antibodies are chemically labeled with the small and large subunits of NanoLuc® Luciferase, known as SmBiT and LgBiT, respectively. In the presence of an analyte, the two antibodies come into close proximity, allowing SmBiT and LgBiT to form an active enzyme and generate a bright luminescence signal. Filter By Lumit™ Assay Type Build Your Own Assay Cytokine Detection HMGB1 Detection Insulin and Glucagon Neonatal Fc Receptor Binding FcγR Binding Protein Phosphorylation SARS-CoV-2 Product Type Detection Reagents Labeling Kit Secondary Antibodies Shop Lumit™ Immunoassay Products Sort by: Newest Alphabetical A-Z Alphabetical Z-A Showing 0 of 15 Products Lumit™ Immunoassays Available by Custom Order In addition to the early-access materials listed above, we offer consultation on development of customized Lumit™ Assays. Please contact us to discuss at the link below to discuss development of a custom solution. Request a Consult What are Lumit™ Immunoassays? Lumit™ Immunoassays are based on the chemical labeling of antibodies and proteins with SmBiT and LgBiT using HaloTag® technology. In the presence of an analyte, the two antibodies come into close proximity and allow SmBiT and LgBiT to form an active enzyme and generate a bright luminescence signal proportional to analyte concentration. They provide a fast alternative to traditional immunoassays, such as ELISA and Western blotting. The Lumit™ Immunoassay Labeling Kit is used to label pairs of antibodies to create Lumit™ Assays. Lumit™ Detection Reagents and pre-labeled LgBiT and SmBiT secondary antibody conjugates are also available. Lumit™ Immunoassays for cytokine detection quantitatively measure target analytes in cell culture samples with a simple, no-wash assay protocol. The assays can be performed directly on cell culture samples or on medium transferred from cell plates. In the Lumit™ Immunoassay Cellular System, the LgBiT and SmBiT subunits are conjugated to a pair of secondary antibodies from two different species. In a multiwell plate, seeded cells are lysed using a NanoBiT®-compatible lysis buffer (digitonin), and the target protein is detected by adding an antibody mix containing two primary antibodies against the protein along with the SmBiT- and LgBiT-conjugated secondary antibodies. The Lumit™ FcRn Binding Immunoassay is a competition assay to measure the interaction between human FcRn and Fc proteins, including antibodies. A Human IgG1 labeled with LgBiT (Tracer-LgBiT) is used as the tracer. A C-terminal biotinylated human FcRn bound to Streptavidin-SmBiT is used as the target. In the absence of an antibody analyte sample, Tracer-LgBiT binds to the hFcRn-SmBiT target resulting in maximum luminescence signal. Unlabeled IgG will compete with Tracer-LgBiT for binding to the FcRn target, resulting in a concentration dependent decrease in luminescent signal. Immunoassay Related Resources Resource White Paper: A Rapid and Sensitive Method for Analyte Detection Webinar Webinar: Lumit™ Immunoassays: An Easier Faster Method for Analyte Detection Resource Paper: A Homogeneous Bioluminescent Immunoassay to Probe Cellular Signaling Pathway Regulation Related Protein Analysis Products and Resources Categories Protein Analysis Protein Detection Protein Interactions Bioassays Related Groups Microplate Readers Protein Quantification Protein Labeling Live-Cell Protein Interactions Guides and Selectors Luminometer and Microplate Reader Selection Guide NanoLuc® Luciferase: One Enzyme, Endless Capabilities Top Products Nano-Glo® HiBiT Extracellular Detection System Nano-Glo® HiBiT Lytic Detection System Let's find the product that meets your needs. Talk to a Scientist Petra Germany Don't miss out! 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