Home About Services Drug Discovery News Careers Contact Receptor Autoradiography In vivo & ex vivo receptor occupancy (RO) offers a simple yet powerful way to measure compound/target engagement using a selective radioligand and receptor autoradiography. Beta – imaging technology, developed by Biospace Lab, France, significantly shortens the time needed for assessment of specific binding in the brain (hours versus weeks for tritiated ligands). Figure 1: Ex vivo receptor occupancy allows to establish correlation between occupancy at the receptor with both in vivo behavioral efficacy and drug exposure. High correspondence between behaviorally active doses of sazetidine in the forced swim test (1A) and α4β2 nicotinic acetylcholine receptor (nAChR) occupancy in the brain (1B). Specifically, sazetidine was inactive in the forced swim test at 0.3 mg/kg (i.p.) and showed very low receptor occupancy at this dose (<10%). In contrast, doses of sazetidine (1 and 3 mg/kg, i.p.) that produced robust behavioral activity in the forced swim test, corresponded with high levels of occupancy at the α4β2 nAChRs (~40% and ~90% respectively). Data published in: Psychopharmacology (Berl). (2011) 217(2): 199-210. Want to get in touch? Connect with the PsychoGenics research team. Connect With Us CONTACT PsychoGenics Inc. 215 College Road, Paramus, NJ 07652 Email: info@psychogenics.com Phone: (914) 406-8019 Fax: (914) 406-8090 Privacy Policy Terms of Use Financial Conflict of Interest Policy Copyright ©2021 PsychoGenics Inc. All rights reserved. We are using cookies to give you the best experience on our website. You can find out more about which cookies we are using or switch them off in settings. Accept