menu The MicroGEM Advantage We’re changing the way people think about molecular biology and sample preparation. Traditional DNA and RNA extraction methods often rely on proteinase K, SDS, and purification by columns, beads, or solvent extraction. Extraneous agents are added that subsequently need to be removed – all in an effort to produce high-quality nucleic acids. Much of the problem is the inadequacy of the lysis step. Proteinase K doesn’t work at a high enough temperature to denature the proteins it is digesting. To compensate, SDS is added but is toxic to every downstream diagnostic. So it must be removed, initiating a cascade of steps – each solving what was done in the previous step. The MicroGEM Advantage Our specially-formulated cocktails of thermophilic and mesophilic enzymes and buffers work at different temperatures, creating coordinated temperature-driven reactions easily done using a thermal cycler or MicroGEM's PDQeX Nucleic Acid Extractor. Our simplified workflow produces high-quality DNA and RNA from a single cell or from thousands of cells – all in minutes, not hours – with good yields, less chance of error, and reduced risk of cross-contamination. Watch MicroGEM's webinar featuring our single-tube approach to effortless nucleic acid extraction and discover how to simplify your workflow. Workflow Comparison Using Temperature-Controlled Extraction vs Conventional Extraction Methods Comparison of MIcroGEM’s temperature-driven, single-tube, simple DNA extraction to conventional approaches using multiple steps, detergents, and denaturants The thermophilic proteinase activates with a simple temperature change. The whole process is easily automated in a single tube using standard laboratory equipment – a standard thermocycler can be programmed to carry out the extraction or use MicroGEM's PDQeX Nucleic Acid Extractor for simple automation. The result? Single-tube nucleic acid extractions, even from challenging samples, compatible with STR, PCR, qPCR and Whole Genome Sequencing without purification steps. Shop by Sample Type Bacteria Plant Cell Culture Sperm RNA Blood Forensic Life Sciences Tissue Forensic Life Sciences Saliva Forensic Life Sciences Instruments and Accessories Home Privacy Policy Shipping Policy Terms & Conditions of Sale Patent Information © 2021. MicroGEM International PLC info[at]microgembio.com Site by Geisler Young Stay connected Sign up for product updates and news from MicroGEM Sign Up