µPAC™ GEN2 Limited Sample Amount column The µPAC™ GEN2 Limited Sample Amount column is designed to maximize protein identifications when working with limited sample amounts. The micro Pillar Array Column technology proves to have exceptional results with regards to reproducibility over time and across laboratories. A second generation design of pillar structure is introduced, where the pillar structure and design have been optimized to enhance the chromatographic separation. With its 50 cm long and 3 µm deep separation channel allied with a non-porous pillars structure, the column is ideally suited for sample loads up to 20 ng of material where ultra-sensitivity is needed. The flow rate range of this column is 50 to 250 nL/min and the optimal gradient time ranges from 30 min to 120 min. The µPAC™ GEN2 Limited Sample Amount column is compatible with nanoViper™ fittings and can be integrated on all commercially available nano-LC systems. Must be used in combination with a grounding cable and has an upper pressure limit of 400 bar. Order now RELATED APPLICATION NOTES All Limited sample Flex iON Connect Next generation non-porous µPAC™ columns for ultra-sensitive nanoLC-MS proteome analysis in limited sample workflows The performance of the µPAC™ GEN2 Limited Sample Amount column is assessed against classic packed-bed columns for the bottom-up proteomics when working with low amount … Read More → RELATED MEDIA All Webinar Publications Technical notes Videos Technical notes Technical note 1: perfect order promotes permeability Perfect order and its role in highly efficient liquid chromatography on micro pillar array columns (µPACTM) – Part 1: Permeability Introduction As stated by Giddings … Read More → Technical notes Technical note 2: µPACTM performance in gradient separations Perfect order and its role in highly efficient liquid chromatography on micro pillar array columns (µPACTM) – Part 2: Performance in gradient separations Introduction As … Read More → Publications μPAC™ column technology for single cell proteomics Read or download the pdf version of this article here. Next to the development of dedicated low-input sample preparation methods, which aim at reducing sample … Read More → Publications Evaluation of Micro-Pillar Array Columns (µPACᵀᴹ) Combined with High Resolution Mass Spectrometry for Lipidomics Evaluation of Micro-Pillar Array Columns (µPACᵀᴹ) Combined with High Resolution Mass Spectrometry for Lipidomics – Koen Sandra et al – LCGC Europe HPLC 2017 Special … Read More → Publications PharmaFluidics and ThermoFisher present new proteomics results at HUPO2017 In collaboration with ThermoFisher, PharmaFluidics has presented new results on the benefits of µPAC™ in the analysis of proteomic samples. The poster, presented at HUPO2017, specifically … Read More → Publications PharmaFluidics and RIC present data on the comparison of biologicals and candidate biosimilars at BioProcess International 2017 At BioProcess International 2017 in Boston (USA), the results of a collaboration between the Research Institute for Chromatography and PharmaFluidics were presented. This poster gives … Read More → 1 2 3 … 5 Next » STAY UP TO DATE ON OUR LATEST INNOVATIONS Subscribe Copyright 2020 PharmaFluidics Technologiepark-Zwijnaarde 82, B-9052 Ghent (Zwijnaarde) T +32 (0)9 241 56 57 Privacy Policy & Disclaimer Cookie settings ACCEPT