About Our People Resources Careers News & Events Contact Drug Development Crop Protection Chemical Safety Drug Development|Phase I support|Pathology Pathology Complete support in necropsy, histotechnology, microscopic evaluation and pathology interpretation for all our toxicology and carcinogenicity studies Our pathology department provides a complete support service for all the company’s toxicology and carcinogenicity studies. Its activities cover necropsy, histopathological processing, microscopic evaluation and pathology interpretation. INTRODUCTION We also offer a comprehensive contract service to many international pharmaceutical and chemical companies operating their own animal testing but relying on external professional support to maximise efficiency or to meet scheduling demands. All work is continually delivered to the highest quality standards, which is achieved by our rigorous in-house quality control programs and highly skilled, experienced technicians and pathologists. We believe in partnerships with our customers, both internally and externally, which results in a personal, professional service from a team who understand the business and the science. PATHOLOGY SERVICES NECROPSY Performed by trained technicians in purpose built suites, necropsies are supervised by experienced pathologists in separate facilities for rodents and non-rodents. Data are entered directly onto a dedicated pathology system HISTOTECHNOLOGY A highly efficient laboratory with automated technology can handle over 500,000 tissues a year. Fully trained staff and intrinsic quality systems ensure the work is produced to the highest standard Specialised Procedures Frozen sectioning Serial / Step sectioning Specialised embedding Photomicrography Immunohistochemistry Morphometric analysis Cell proliferation assays Special stains Archive ready packaging of residual wet tissues Microscopic Evaluation Sequani employs three full time toxicological pathologists with combined experience amounting to over 60 years. The qualifications of our pathologists are detailed below: Pathologist A: BDS(Edin) DipRCPath (Tox) FRCPath Pathologist B: BSc(Hons) PhD DipRCPath (Tox) FRCPath Pathologist C: DVM MRCVS Statistical analysis is overseen by one of the leading UK experts in this field.Sequani pathologists have special expertise in respiratory, endocrine, cardiovascular, ocular and dermal pathology, carcinogenicity studies, transgenic mice, juvenile organ development and toxicity in the juvenile brain. FOETAL EVALUATION A dedicated team of foetal morphologists, with vast experience in all aspects of foetal evaluation, undertakes all of the skeletal, and Bouins examination from studies undertaken at Sequani which ensures that we have consistency in reporting levels. Single and double skeletal staining techniques are fully validated and routinely performed using our suite of Shandon Pathcentre Teratology Processors. SPERM ANALYSIS We have the expertise and equipment to provide extensive analysis of sperm morphology, motility and concentration in rats. We use the Hamilton Thorne TOX IVOS software to produce reports detailing percentage motility and a range of velocity parameters from live epididymal samples as well as concentration data from both epididymal samples and homogenisation resistant spermatids from the testis. In addition we perform a differential morphological analysis detailing any gross abnormalities. OVARIAN FOLLICLE COUNTS We can perform counts for naked oocytes and small and medium follicles in serial ovarian sections taken from the rat. This can be achieved using standard Haematoxylin and Eosin staining or using immunohistochemical staining for PCNA. IMMUNOCYTOCHEMISTRY Method feasibility Method validation Slide preparation and evaluation CONSULTANCY Necropsy Histology Immunohistochemistry Peer Review CONTACT US LET'S FOCUS OUR NEWS & EVENTS European Teratology Society (ETS) 11 September 2014 Richard Bartlett appointed Secretary of the European Teratology Society READ MORE Celebrating a commitment to training 12 August 2014 Sequani's training is recognised for its quality and scope READ MORE Meet the Sequani team across Europe via our Autumn conference schedule 31 July 2014 Our experts will be at major meetings and conferences throughout the Autumn. Do come and see us to hear about our latest work and discuss forthcoming ... READ MORE VIEW ALL ARTICLES RELATED SPECIALISMS Phase I support NON CLINICAL STRATEGY Making use of the vast experience of our senior scientists and programme managers to design thorough and effective studies. Phase I support GENERAL TOXICOLOGY Quality studies in rodent and non-rodent species delivered on time to meet your product and international regulatory needs Phase I support GENETIC TOXICOLOGY A comprehensive range of genetic toxicity services for preclinical assessment and the early screening of candidate compounds Phase I support PATHOLOGY Complete support in necropsy, histotechnology, microscopic evaluation and pathology interpretation for all our toxicology and carcinogenicity studies ‹ › VIEW ALL great people, great work, real results HOW CAN WE HELP? Tel.: +44 1531 634121 Fax: +44 1531 634753 Sequani Limited, Bromyard Road, Ledbury HR8 1LH United Kingdom CONTACT US Terms of Use | Privacy