µPACᵀᴹ capLC column The µPACᵀᴹ capLC column serves those who are looking for increased robustness and throughput without losing sensitivity. A flow rate versatility between 1 and 15 µL/min at moderate pressures enables short gradient separations. The µPAC™ technology ensures an exceptionally high reproducibility over time and across laboratories. This makes the µPAC™ capLC column ideally suited for applications such as (clinical) proteomics, metabolomics and biopharmaceutical analyses. The column is compatible with all commercially available capillary LC systems, and can be integrated smoothly in any experimental set-up. Must be used in combination with a grounding cable. Order now This column offers: Optimal separation performance Introducing perfect order in the chromatographic separation bed guarantees minimal peak dispersion resulting in high peak capacities and increased proteome coverage. Discover the results in our application note about peptide mapping. Easy to implement Plug and play connectivity by introducing commonly used 1/16” fittings from both sides, makes the column compatible with any third party LC vendor. Extreme robustness Each column has been manufactured by etching channels and arrays of pillars out of a solid silicon wafer. The column offers a high permeability and contains no particles or frits. This prevents blockages and retention time variation over multiple runs. Run your entire project on a single column. Increased column to column reproducibility The microfabrication production process, using the same lithographic mask, ensures that every column is identical. Replace µPAC™ capLC columns without shifts in retention time or differences in performance. Obtain results over time and across laboratories, sufficiently consistent for big data analysis. High flow rate flexibility The capLC column can be operated at moderate LC pump pressures up to 350 bar over a wide range of flow rates between 1 and 15 µL/min. The entire capillary flow segment is covered with a single column as demonstrated in this webinar. RELATED APPLICATION NOTES All capLC 50cm Detailed glycosylation analysis of therapeutic enzymes using µPAC™ capLC-MS and all-ion fragmentation The use of micro Pillar Array Columns (μPAC™) for characterizing glycosylation of therapeutic enzymes is presented. Recombinant human acid α-glucosidase (hGAA) was digested and resulting … Read More → capLC High-throughput, routine and comprehensive proteome analysis using a micro Pillar Array Column (μPAC™) based capillary-flow LC-MS workflow In this application note, optimal LC methods for a variety of LC-MS based proteome analysis needs are described. PharmaFluidics’ μPAC™ capLC column is evaluated for … Read More → capLC Bottom-Up Proteomic Profiling at Capillary LC Flow Rates In this application note, the performance of PharmaFluidics’ μPAC™ capLC columns is evaluated in a typical bottom-up proteomics workflow. 500 ng of tryptic digest sample … Read More → RELATED MEDIA All Webinar Publications Technical notes Videos Webinar Webinar – Capillary Flow LC-MS using µPAC™ columns-Combining Sensitivity with Robustness and Throughput Capillary Flow LC-MS using µPAC™ columns-Combining Sensitivity with Robustness and Throughput Mass-spectrometry based proteomics has become an essential tool in biological, biomedical and biopharmaceutical research. … Read More → Webinar Webinar – Micro Pillar Array Columns for Analysis of Physiological Fluids Micro Pillar Array Columns for Analysis of Physiological Fluids Micro-pillar arrays provide a highly ordered chromatographic solid phase which minimizes peak dispersion through variability in … Read More → Webinar Webinar – A New LC Approach to Complex Biological Samples by using micro Pillar Array Columns A New LC Approach to Complex Biological Samples by using micro Pillar Array Columns In the last decades, several advances have been made in liquid … Read More → Webinar Webinar – Capillary Flow LCMS Using micro Pillar Array Columns Combining Nano Flow Sensitivity with Analytical Flow Robustness and Throughput In this webcast, Dr. Jeff Op de Beeck of PharmaFluidics will explain the principles of the micro-chip based pillar array columns and demonstrate how the … Read More → Webinar Webinar – Practical applications of µPAC™ column technology using data-independent acquisition for clinical proteomics In this webcast, several workflows will be shown. The first part will discuss our collaboration with the researchers from Biognosys, working on a comprehensive proteomics … Read More → Webinar Webinar – Advanced proteomic workflows using micro Pillar Array Columns: Insights of ‘The proteome landscape of the kingdoms of life’ In this webcast, Dr. Geert Van Raemdonck of PharmaFluidics will explain the principles of the micro-chip based pillar array columns and demonstrate how the PharmaFluidics … Read More → 1 2 Next » STAY UP TO DATE ON OUR LATEST INNOVATIONS Subscribe Copyright 2020 PharmaFluidics Technologiepark-Zwijnaarde 82, B-9052 Ghent (Zwijnaarde) T +32 (0)9 241 56 57 Privacy Policy & Disclaimer Cookie settings ACCEPT