HomeProductsMolecular Biology & Functional GenomicsGene Editing & Functional GenomicsCRISPR & Gene Editing CRISPR & Gene Editing THE ULTIMATE SUITE OF GENOME ENGINEERING TOOLS AND SERVICES Advances in genome engineering are enabling researchers to achieve unprecedented breakthroughs, from curing genetic disorders to developing disease-resistant crops. Sigma-Aldrich® Advanced Genomics delivers complete support for every step of CRISPR-based gene editing research at any scale. Target a single gene using Zinc Finger Nucleases or screen a whole genome with fully customizable CRISPR products. With over 350 years of technical leadership, Sigma-Aldrich® Advanced Genomics products come with expertise that extends Beyond the Bench - from discovery to therapeutic impact. The Sigma-Aldrich® Advanced Genomics portfolio delivers the ultimate suite of genome engineering tools and services to unlock your research potential. Whether you are looking to knockout, knock-in, knockdown, or overexpress your targets, our comprehensive suite of CRISPR gene-editing tools and services will take your research Beyond the Bench. Order Cas9 Proteins Order Pre-designed gRNA Order Custom gRNA Design your CRISPR gRNA BEST IN CLASS Cas9 PROTEINS Sigma-Aldrich® Advanced Genomics offers multiple Cas9 variants (wild-type, enhanced specificity, nickase, GFP-fused, catalytically inactive) and in several formats (plasmid, lentivirus, lyophilized protein). Choose from an industry leading selection of Cas9 proteins to meet your individual research needs. Explore our full list of Cas9 Products Learn more about CRISPRi and CRISPRa Gene Modulation Learn more about Cas9-GFP Fusion Proteins GUARANTEED CRISPR gRNA Every SygRNA® synthetic single guide RNA (sgRNA), crRNA and tracrRNA is synthesized using our proprietary process and reagents using the highest quality materials available. Editing activity is assured regardless of your target, cell type or application, and is backed by the most comprehensive guarantee in the industry. Order pre-designed synthetic CRISPR guide RNA (sgRNA) Order custom synthetic CRISPR RNA Learn more about SygRNA® synthetic CRISPR guides CRISPR INHIBITION AND ACTIVATION (CRISPRi AND CRISPRa) Gain even greater insights into complex biological networks using Sigma-Aldrich® Advanced Genomics CRISPR activation and CRISPR inhibition libraries for gene knockdown and overexpression experiments. Pooled CRISPRi and SAM CRISPRa lentiviral libraries are available off-the-shelf or customized to your specific needs. Learn more about CRISPRi & CRISPRa Genetic Screening Determining Which Screening Option is Right for Your Experiment CRISPRi Whole Genome and Non-Coding Libraries CRISPRa Whole Genome SAM Libraries CRISPR SCREENING WITH 10X GENOMICS COMPATIBILITY Sigma-Aldrich® Advanced Genomics offers the only 10x Genomics Certified Compatible lentiviral pools for CRISPR single-cell screening. Our optimized, custom lentiviral vectors are fully compatible with Chromium Single Cell Gene Expression v3 and v3.1 (Next GEM) solution utilizing Feature Barcode technology. Learn more about 10x Genomics Compatible products. WE’RE HERE TO HELP Whether you need help designing your CRISPR experiment, require assistance troubleshooting, or simply want to better understand how you can apply gene editing methods in your research, Sigma-Aldrich® Advanced Genomics is here to guide you. Wondering if CRISPR is right for your project? Connect with a Sigma-Aldrich® Advanced Genomics Expert. PRODUCTS Showing 1-20 of 50 Filter & Sort CAS9PROT Cas9 Protein, from Streptococcus pyogenes, recombinant, expressed in E. coli, 1X NLS View Pricing CAS9GFPPRO Cas9-GFP Protein, from Streptococcus pyogenes, fused with enhanced GFP, recombinant, expressed in E. coli, 3X NLS View Pricing SITRAN-RO X-tremeGENE™ siRNA Transfection Reagent, Polymer reagent for delivering siRNA to common cell lines View Pricing CAS9P Cas9 plasmid View Pricing DCAS9PROT dCas9-3XFLAG™-Biotin Protein, from Streptococcus pyogenes with D10A and H840A mutations, recombinant, expressed in E. coli, 1X NLS View Pricing CAS9MRNA Cas9 mRNA View Pricing ECAS9GFPPR eSpCas9-GFP Protein, from Streptococcus pyogenes with mutations conferring enhanced specificity, fused with enhanced GFP, recombinant, expressed in E. coli, 3X NLS View Pricing ESPCAS9PRO eSpCas9 Protein, from Streptococcus pyogenes with mutations conferring enhanced specificity, recombinant, expressed in E. coli, 1X NLS View Pricing CAS9GFPP CMV-CAS9-2A-GFP Plasmid View Pricing LVCAS9BST CAS9 Blasticidin Lenti Particles View Pricing CAS9D10APR Cas9-D10A Nickase Protein, from Streptococcus pyogenes with D10A mutation, recombinant, expressed in E. coli, 1X NLS View Pricing CAS9D10AP CRISPR Cas9-D10A Nickase Plasmid View Pricing TRACRRNAMOD SygRNA® Cas9 Synthetic Modified tracrRNA View Pricing CAS9GFPV Cas9 GFP Lentiviral Particles View Pricing CAS9RFPP CMV-CAS9-2A-RFP Plasmid View Pricing CAS9BST CAS9 Blasticidin Lenti Plasmid View Pricing FNCAS9PROT FnCas9 Protein, from Francisella novicida, recombinant, expressed in E. coli, 1X NLS View Pricing CAS9GFPVP Cas9-GFP Lenti Plasmid View Pricing CTI1 CompoZr® Targeted Integration Kit - AAVS1, Transgene Integration in the Human Genome View Pricing CAS9HYGROV Cas9 Hygromycin Lentiviral Particles View Pricing Page 1 of 3 RELATED PRODUCT RESOURCES Cas9-Mediated Recombineering Enhances Metabolic Production of Kdo2-Lipid A by E. coli In this article, we present an application of our novel E. coli CRISPR/Cas-mediated Lambda-Red (λ-Red) homologous recombination (HR) vector system, which facilitates gene editing through the homology-directed repair (HDR) of double-stranded DNA breaks (DSBs) created by Cas9 endonuclease, using either ssDNA or dsDNA as an editing template. CRISPR Use License Agreement This Product and its use are the subject of one or more of the following patents controlled by The Broad Institute, Inc. (BROAD), the Massachusetts Institute of Technology (MIT), or the President and Fellows of Harvard College (HARVARD): U.S. Patent Nos. 8,697,357; 8,771,945, and any substitutions, divisions, continuations, reissues, renewals, re-examinations or extensions, and corresponding foreign patent applications and patents. What Is ZFN Technology? Zinc finger nucleases (ZFNs) are a class of engineered DNA-binding proteins that facilitate targeted editing of the genome by creating double-strand breaks in DNA at user-specified locations. How Do ZFNs Work? CompoZr Zinc Finger Nucleases (ZFNs) are used to create modified cell lines with targeted gene deletions, gene insertions, or gene corrections. Targeted Genome Editing Using Engineered Zinc Finger Nucleases Targeted genome editing with Zinc finger nucleases (ZFNs) has a wide variety of applications, and three key modes of action have been a focus thus far: targeted gene knockouts, targeted gene integration, and targeted gene correction. RELATED PROTOCOLS CRISPR/CAS9 Gene Editing Protocol for Human Induced Pluripotent Stem Cells (iPSCs) This article provides an extensive step-wise overview of CRISPR Cas9 protocol that can be used to perform gene editing in human induced pluripotent stem cells (iPSCs). Ribonucleoprotein (RNP) Protocols using SygRNA® gRNAs and Cas9 proteins Combine guaranteed sgRNAs with our comprehensive range of CRISPR products and tools, including Cas9 and delivery reagents, for efficient genome modification with higher specificity. Genome Editing in Plants with CRISPR/Cas9 Zinc finger nucleases (ZFNs), were introduced by Sigma-Aldrich less than 8 years ago, but in that time the technology of targeted genome editing has advanced rapidly. Most recently, the discovery of the CRISPR/Cas9 pathway has accelerated interest in this field, opening up new possibilities for research and development. RELATED PRODUCT CATEGORIES Genetic Screening We have the most comprehensive selection of screening tools in pooled and arrayed CRISPR, RNAi, and ORF formats. Whole-genome and custom designed libraries in arrayed or pooled formats meet all of your functional genomics screening needs at any scale. RNAi Single Clones Genome-wide human and mouse shRNA clones from the TRC1.5 and TRC2 collections, available as plasmids or high-titer virus. Efficient and stable gene knockdown with expert support and customization options available in a variety of vector backbones. RNAi Libraries Human and mouse lentivirus shRNA screening libraries and pools from the RNAi Consortium (TRC). Available as bacterial glycerol stocks, plasmid DNA or read-to-use lentiviral particles. MISSION® RNAi libraries are also available with a range of custom vectors, volumes and viral titers. RELATED SERVICES Cell Design Studio® Cell Engineering Services Cell Design Studio® is the leading provider of custom cell lines. Sigma-Aldrich® Advanced Genomics genome engineers can deliver even the most challenging edits in any cell line Genomic DNA Prep & Deconvolution Services Need help with deconvolution of pooled shRNA samples after screening is complete? We provide a pooled shRNA deconvolution service that will help you identify TRC clones that are of significance in your screen. HIGHLIGHTS Gene Editing 101 Book A free eBook designed to help you understand, design, and perform CRISPT experiments. Download your copy now! © 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. 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