Home About Carbosynth Basket Our Distributors Careers Contact Us <<back 2-Nitrophenyl b-D-galactopyranoside CAS No: 369-07-3 Synonyms: ONPG Product Code: EN06363 MDL No: MFCD00063255 Chemical Formula: C12H15NO8 Molecular Weight: 301.25 Product Structure References: 1. Maruhn D, Clin. Chim. Acta 1976, 73, 453 2. Levvy GA, Conchie J, Methods Enzymol. 1966, 8, 571-84 3. Naider F, et al., Biochemistry 1972, 11, 3202-7 Click to load specification Pack Size Pack Price Hazardous Surcharge Special Packaging Number of Packs 10g $38.00 Not Applicable Not Applicable Check Availability 25g $65.00 Not Applicable Not Applicable Check Availability 50g $85.00 Not Applicable Not Applicable Check Availability 100g $125.00 Not Applicable Not Applicable Check Availability 250g $270.00 Not Applicable Not Applicable Check Availability Custom Pack -Unit- g $0.00 Not Applicable Not Applicable Enter a pack size. Scale Available 100’s kgs For requirements greater than the catalogue prepacks email: enquiries@carbosynth.com Custom Synthesis For custom synthesis or special requests email: enquiries@carbosynth.com, call +44 (0)1635 578444 or fill out our contact form ONPG ONPG is the common abbreviation for (ortho) 2-Nitrophenyl-β-D-galactopyranoside. ONPG is used in colourimetric and spectrophotometric assays for the detection of β-galactosidase activity which are of interest in clinical, environmental, food and molecular biology arenas.1 ONPG is hydrolysed by β-galactosidase to yield galactose and ortho-Nitrophenol, which is yellow in colour and absorbs light at 420 nm. The intensity of the colour can be used to determine the rate at which β-galactosidase can hydrolyse lactose. The impact of experimental factors on the reproducibility of β-galactosidase assays has been reported.2 In common with several other β-galactosidase substrates such as X-Gal, ONPG does not act as an inducer of genes under the control of the lac operon in bacteria such as E. coli. In the absence of a lactose analogue such as IPTG (Product Code: E10593 I) transcription of β-galactosidase will not take place and ONPG will not be hydrolysed. Refs: 1. J Lederberg, J. Bacteriol., 1950, 20, 137. 2. A Giacomini et al, FEMS Microbiol. Lett., 2006, 100, 87. Products Carbohydrates Monosaccharides Oligosaccharides Enzyme substrates Detergents All - Alphabetically Nucleosides Nucleosides Phosphoramidites Fine Chemicals Fine Chemicals Antibiotics Antibiotics Featured Products 2-Chloro-4-nitrophenyl a-L-fucopyranoside PUGNAc X-Gal ONPG IPTG Non-animal Origin EDC.HCl, EDAC.HCl HOPO The Schöllkopf Chiral Auxiliary: (S) The Schöllkopf Chiral Auxiliary: (R) N-Acetylneuraminic acid All featured products... Navigation Home About Carbosynth Our Distributors Basket Contact Us Sitemap Carbosynth Limited 8 & 9 Old Station Business Park - Compton - Berkshire - RG20 6NE - UK Tel: +44 (0)1635 578444- Fax +44 (0)1635 579444 email: sales@carbosynth.com | terms and conditions Product Name Product Code Keyword Full Text CAS No MDL No carbohydrates nucleosides fine chemicals antibiotics featured products Your Basket Number of Items 0 Value 0.00 Our Catalogues NEW