Proteomics Application notes µPAC™ Generation 2: setting a new standard in nanoLC column performance The performance of the 50 cm µPAC™ GEN2 Nano column is demonstrated on LCMS instrumentations of different sensitivity levels. The column is also assessed against state-of-the-art packed bed columns… Read More Next generation non-porous µPAC™ columns for ultra-sensitive nanoLC-MS proteome analysis in limited sample workflows The performance of the µPAC™ GEN2 Limited Sample Amount column is assessed against classic packed-bed columns for the bottom-up proteomics when working with low amount of sample on an… Read More High-throughput, routine and comprehensive proteome analysis using a micro Pillar Array Column (μPAC™) based capillary-flow LC-MS workflow In this application note, optimal LC methods for a variety of LC-MS based proteome analysis needs are described. PharmaFluidics’ μPAC™ capLC column is evaluated for different user needs ranging… Read More Bottom-Up Proteomic Profiling at Capillary LC Flow Rates In this application note, the performance of PharmaFluidics’ μPAC™ capLC columns is evaluated in a typical bottom-up proteomics workflow. 500 ng of tryptic digest sample (HeLa cell lysate) was… Read More Sensitive detection of peptides in Cytochrome C digest using nano LC with micro Pillar Array Columns (µPACᵀᴹ) and Microsaic 4500 MiD Mass Spectrometer Liquid chromatography coupled with mass spectrometry is a powerful analytical tool for detection and identification of chemicals in complex mixtures. The Microsaic 4500 MiD® is a miniaturised single quadrupole mass… Read More Peptide separation on micro pillar array columns (µPACᵀᴹ). Part 1: Cytochrome C tryptic digest Reversed phase cytochrome c tryptic digest separations are routinely employed for the analysis/quality control of HPLC column performance towards proteomics applications. Easily detectable in UV and with few components,… Read More Technical notes Technical note 9: µPAC™ Flex iON Connect ESI-MS interface µPAC™ Flex iON Connect ESI-MS interface The μPAC™ Flex iON Connect is a user-friendly interface to facilitate fast, reproducible and low dispersion connection of μPAC™ nanoLC columns to Thermo… Read More Technical note 8: µPAC™ LC, a new era in column-to-column reproducibility µPAC™ LC, a new era in column-to-column reproducibility The main goal of this study is to compare the column-to-column reproducibility of the μPAC™ capLC column format to state-of-the art… Read More Technical note 7: Assessment of capillary flow HPLC column performance Assessment of capillary flow HPLC column performance In this technical note, the performance of PharmaFluidics µPAC™ capLC columns is compared to the current state-of-the-art in commercial capillary flow LC… Read More Technical note 6: Peptide sample enrichment using μPAC™ Trapping columns Peptide sample enrichment using μPAC™ Trapping columns The online preconcentration of analytes onto low volume trapping columns is a commonly used injection strategy in nano- and microbore LC-MS/MS analysis… Read More Technical note 5: Routine proteome analysis using 50 cm μPAC™ columns Routine proteome analysis using 50 cm μPAC™ columns The practice of bottom-up proteomics relies to a large extent on the separation performance that can be achieved with state-of-the art… Read More Technical note 4: μPAC™ column performance with peptide standards: Thermo Scientific™ Pierce™ retention time calibration mixture μPAC™ column performance with peptide standards: Thermo Scientific™ Pierce™ retention time calibration mixture Peptide standards are critical in mass spectrometry based proteomics to ensure optimal and consistent system performance… Read More Technical note 3: μPAC™ column robustness in bottom-up proteomics μPAC™ column robustness in bottom-up proteomics Next to technical issues that can occur with the LC-MS system’s hard- and software, LC column failure is one of the most frequent… Read More Webinars Webinar – Practical applications of µPAC™ column technology using data-independent acquisition for clinical proteomics In this webcast, several workflows will be shown. The first part will discuss our collaboration with the researchers from Biognosys, working on a comprehensive proteomics method in Data Independent… Read More Webinar – Advanced proteomic workflows using micro Pillar Array Columns: Insights of ‘The proteome landscape of the kingdoms of life’ In this webcast, Dr. Geert Van Raemdonck of PharmaFluidics will explain the principles of the micro-chip based pillar array columns and demonstrate how the PharmaFluidics µPAC™ columns can contribute… Read More Webinar – Micro Pillar Array Columns for Analysis of Physiological Fluids Micro Pillar Array Columns for Analysis of Physiological Fluids Micro-pillar arrays provide a highly ordered chromatographic solid phase which minimizes peak dispersion through variability in path lengths through the… Read More Webinar – Tips and tricks when working with PharmaFluidics’ micro Pillar Array Columns Tips and tricks when working with PharmaFluidics’ micro Pillar Array Columns Compared to traditional LC columns, PharmaFluidics uses a fundamentally different approach to fabricate their chromatographic columns. Instead of… Read More Webinar – Capillary Flow LC-MS using µPAC™ columns-Combining Sensitivity with Robustness and Throughput Capillary Flow LC-MS using µPAC™ columns-Combining Sensitivity with Robustness and Throughput Mass-spectrometry based proteomics has become an essential tool in biological, biomedical and biopharmaceutical research. Due to the increased… Read More Webinar – A Novel Nanoflow LC–MS Approach for Bottom-up Proteomics Using the 200 cm long µPAC™ column A Novel Nanoflow LC–MS Approach for Bottom-up Proteomics Using the 200 cm long µPAC™ column Bottom-up proteomics using 50 to 100 µm C18 packed capillaries coupled to high resolution… Read More Webinar – Expanding the limits of routine proteomics using 50 cm micro Pillar Array Column (µPAC™) Expanding the limits of routine proteomics using 50 cm micro Pillar Array Column (µPAC™) The practice of bottom-up proteomics relies to a large extent on the separation performance that… Read More Webinar – A New LC Approach to Complex Biological Samples by using micro Pillar Array Columns A New LC Approach to Complex Biological Samples by using micro Pillar Array Columns In the last decades, several advances have been made in liquid chromatography (LC) column technology…. Read More Webinar – Capillary Flow LCMS Using micro Pillar Array Columns Combining Nano Flow Sensitivity with Analytical Flow Robustness and Throughput In this webcast, Dr. Jeff Op de Beeck of PharmaFluidics will explain the principles of the micro-chip based pillar array columns and demonstrate how the PharmaFluidics µPAC™ capLC column… Read More Watch our webinar on demand – Double your IDs for single cell proteomics using micro Pillar Array Columns (µPAC™) This webinar will explore the potential of newly emerging chromatographic technologies to significantly improve sensitivity for low input proteomics samples, as well as providing detailed experimental results as evidence…. Read More Webinar – Expanding the Limits of Shotgun Proteomics Using Micro Pillar Array Columns and Data-Independant Acquisition (DIA) Methods In this webinar, Dr. Jeff Op de Beeck, the application development manager of PharmaFluidics, will explain specific features of the micro-chip-based pillar array chromatography and Dr. Jan Muntel, a… Read More Webinar – Using Micro Pillar Array Columns in Clinical Proteomics Setup – A Novel Nanoflow LC–MS Approach Watch this webinar to hear, Dr. Jeff Op de Beeck, the Application Development Manager of PharmaFluidics, explain the µPAC™ technology and Dr. Goran Mitulović, head of the Proteomics Core… Read More Webinar: A novel nanoflow LC-MS approach for bottom-up proteomics using micro Pillar Array Columns Watch this webinar to hear, Dr. Jeff Op de Beeck, the Application Development Manager of PharmaFluidics, explain theµPAC™ technology. He is joined by Dr. Francis Impens, the Manager of… Read More Publications Executive Summary – Advanced Proteomic Workflows Using micro Pillar Array Columns: The Proteome Landscape of the Kingdoms of Life LCGC wrote an executive summary about our webinar with Johannes Müller (Max Planck Institute of Biochemistry, Mann group), ‘Advanced Proteomic Workflows Using micro Pillar Array Columns: The Proteome Landscape… Read More Duncan Smith – Robustness and repeatability The promise of μPAC™ in low flow LCMS Interview with Dr. Duncan Smith, Cancer Research UK (CRUK) Manchester Institute. Within Cancer Research UK Manchester Institute, Dr. Smith is Head of Mass Spectrometry, responsible for innovative biological mass… Read More Executive Summary – Capillary Flow LCMS Using Micro Pillar Array Columns Combining Nano Flow Sensitivity with Analytical Flow Robustness and Throughput LCGC wrote an executive summary about our webinar with ProGenTomics, Capillary Flow LCMS Using Micro Pillar Array Columns Combining Nano Flow Sensitivity with Analytical Flow Robustness and Throughput. Our… Read More Andrea Sinz – How to overcome challenges with XL-MS to study whole proteomes In this exclusive interview, Prof. Dr. Andrea Sinz discusses the steps her group has taken to move from single protein studies to complex networks and how they are hoping… Read More Routine Proteome Analysis Using 50 cm micro Pillar Array Columns – GEN According to PharmaFluidics, more sample components can be identified if separation channels are etched in silicon. The practice of bottom-up proteomics relies to a large extent on the separation… Read More Executive Summary LCGC – Expanding the Limits of Shotgun Proteomics Using Micro-Pillar Array Columns and Data-Independent Acquisition Methods LCGC wrote an executive summary about our webinar with Biognosys, Expanding the Limits of Shotgun Proteomics Using Micro-Pillar Array Columns and Data-Independent Acquisition Methods. Our Application Development Manager, Dr…. Read More Perfecting Proteomics Separations An innovative award-winning micro-chip chromatography ‘column’, µPACᵀᴹ promises high-resolution separations of complex biological samples. Prof Kris Gevaert and Dr Francis Impens, from Ghent University and VIB Proteomics Core Facility,… Read More Executive Summary LCGC – A Novel Nanoflow LC-MS Approach for Bottom-up Proteomics Using Micro Pillar Array Columns LCGC wrote an executive summary about our webinar, A Novel Nanoflow LC-MS Approach for Bottom-up Proteomics Using Micro Pillar Array Columns. Our Application Development Manager, Dr. Jeff Op de… Read More µPACᵀᴹ in practice – Part II: Goran Mitulović µPACᵀᴹ in practice – The Analytical Scientist, November, 2017 Part II: An interview with Goran Mitulović, Head of the Proteomics Core Facility at the Medical University of Vienna, Austria… Read More PharmaFluidics and ThermoFisher present new proteomics results at HUPO2017 In collaboration with ThermoFisher, PharmaFluidics has presented new results on the benefits of µPAC™ in the analysis of proteomic samples. The poster, presented at HUPO2017, specifically demonstrates that: Highly reproducible… Read More STAY UP TO DATE ON OUR LATEST INNOVATIONS Subscribe Copyright 2020 PharmaFluidics Technologiepark-Zwijnaarde 82, B-9052 Ghent (Zwijnaarde) T +32 (0)9 241 56 57 Privacy Policy & Disclaimer Cookie settings ACCEPT