Cart Applications HPLC/LC-MS We offer complete reagent sets for the quantification of vitamin status, oxidative health and metabolic disease. What is it? Pre & Post-Column Common Types What is HPLC? HPLC stands for High-Performance Liquid Chromatography. It uses the chemical properties of a stationary phase (the column) and a mobile phase (the buffer) to separate complex mixtures of molecules. The column serves to slow the movement of compounds to varying degrees based on their chemical or physical properties. By varying the properties of the two phases and the runtime parameters, different compounds can be separated in different ways. The column parameters can vary based on particle or pore size, internal diameter, column length and flow rate through the column. The buffer can vary based on composition (differing levels of aqueous or organic solvents) or by changing the solvent concentration over time (isocratic versus gradient). Search Browse Therapeutic Areas HPLC/LC-MS Shop Products HPLC/LC-MS FAQs Tips & Tricks Related Resources Related Articles + Vitamin D Metabolism and Function Read More 1 2 Technical Literature Handouts Browse Our HPLC/LC-MS Products Shop Now Applications Browse our helpful resources and product offerings by application. Immunoassays Providing a diverse offering of high quality assays to the life science community for over 20 years. Learn More HPLC/LC-MS Complete reagent sets for quantification of vitamin status, oxidative stress, and metabolic disease. Learn More Chemiluminescence Offering highly sensitive and accurate broad range assays, saving laboratories time and money. Learn More Sign up for news, tips, promotions and more. 800.592.5726 Contact