Products Area of Interest Resources Support Company Careers Publications AREA OF INTEREST Neuroscience (3) Assay Method (1) Cancer Research (1) Developmental Biology (1) Genetic Health (1) Infectious Disease (1) Reproductive Biology (1) SPECIES Human (5) Mouse (1) TISSUE TYPE Blood (2) Brain (1) Colon (1) Organoid (1) Reproductive organ (1) Tumor - solid (1) JOURNAL Cell Reports (1) Genome research (1) Nature Biotechnology (1) Nature Immunology (1) 10X GENOMICS PRODUCT Single Cell Gene Expression (4) Single Cell Immune Profiling (2) Single Cell ATAC (1) Show more filters Show Only Featured Publications (1) 6 RESULTS FOR "32788748" Sort by: Nature Immunology, Aug 2020 Single-cell landscape of immunological responses in patients with COVID-19 Single Cell Immune Profiling DOI: 10.1038/s41590-020-0762-x PubMed: 32788748 ABSTRACT In coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, the relationship between disease severity and the host immune response is not fully understood. Here we performed single-cell RNA sequencing in peripheral blood samples of 5 healthy donors and 13 patients with COVID-19, including moderate, severe and convalescent cases. Through determining the transcriptional profiles of immune cells, coupled with assembled T cell receptor and B cell receptor sequences, we analyzed the functional properties of immune cells. Most cell types in patients with COVID-19 showed a strong interferon-α response and an overall acute inflammatory response. Moreover, intensive expansion of highly cytotoxic effector T cell subsets, such as CD4effector-GNLY (granulysin), CD8effector-GNLY and NKT CD160, was associated with convalescence in moderate patients. In severe patients, the immune landscape featured a deranged interferon response, profound immune exhaustion with skewed T cell receptor repertoire and broad T cell expansion. These findings illustrate the dynamic nature of immune responses during disease progression. AUTHORS Ji-Yuan Zhang, Xiang-Ming Wang, Xudong Xing, Zhe Xu, Chao Zhang, Jin-Wen Song, Xing Fan, Peng Xia, Jun-Liang Fu, Si-Yu Wang, Ruo-Nan Xu, Xiao-Peng Dai, Lei Shi, Lei Huang, Tian-Jun Jiang, Ming Shi, Yuxia Zhang, Alimuddin Zumla, Markus Maeurer, Fan Bai, Fu-Sheng Wang View on PubMed AREA OF INTEREST Infectious Disease SPECIES Human SAMPLE TYPES Cells from Blood