Home Applications Applied Sciences Plant Pathogen Detection Plant pathogens that cause plant diseases can harm our agricultural ecosystems and have devastating impacts on our livelihoods. Scientists studying plant pathology or phytopathology in agriculture play a key role in monitoring and managing plant diseases. To support their efforts, we provide molecular tools that can rapidly and accurately detect and diagnose plant pathogens such as viruses, viroids, bacteria, fungi and yeast. Need faster and more reliable plant pathogen detection? We’re here to help! Request a Consult Plant Pathogen DNA/RNA Extraction The key to managing the spread of plant pathogens is rapid and accurate detection. Plant pathogen detection begins with extracting DNA or RNA from plant samples, including leaf, seed or root. High-quality DNA is essential for achieving reproducible results in downstream qPCR or next-generation sequencing (NGS) methods. Our Maxwell® Instruments and nucleic acid extraction kits allow automated extraction of up to 48 plant samples at once. DNA/RNA is purified in less than one hour and can be used directly in qPCR and other downstream detection methods. Protocols for Plant Pathogen Nucleic Acid Purification Find protocols below for extracting plant pathogen DNA/RNA from infected plant tissue. Can’t find what you need? Contact our Technical Services team and they’ll work with our Applications Scientists to develop a solution just for you! Download Xylella fastidiosa Extraction ProtocolDownload Phytoplasma Extraction Protocol Maxwell® Kits for Plant Pathogen DNA/RNA Extraction Maxwell® RSC PureFood GMO and Authentication Kit Safe, automated DNA purification from food and feed samples. AS1600 Maxwell® RSC Plant RNA Kit Extracts RNA from plant tissue samples using the Maxwell® RSC Instrument. AS1500 Maxwell® RSC Plant DNA Kit Extracts DNA from plant tissue samples using the Maxwell® RSC Instrument. AS1490 Plant Pathogen PCR Detection Our qPCR and RT-qPCR products can detect and amplify target sequences of plant pathogens to accurately diagnose plant diseases. We provide custom made-to-order PCR master mixes to detect your plant pathogen of interest, and the PCR Optimization Kit can help you determine the optimal amplification condition. PCR Detection Kits GoTaq® Probe qPCR and RT-qPCR Systems Ready-to-use master mixes for probe-based qPCR or RT-qPCR. A6101, A6102, A6120, A6121, A6110 PCR Optimization Kit Preformulated PCR master mix components to determine optimal PCR amplification conditions. D2381 Xylella fastidiosa Detection Methods Xylella fastidiosa is a plant pathogen that has caused immense destruction to our environment and economy. This bacterium is spread by insects and infects plant xylem, the tissue that transports nutrients from roots to leaves, causing the plant to starve and die within 1–2 years. Currently there is no cure for X. fastidiosa infection, and it has now spread across the globe. In Europe, 20 million olive trees have been infected, devastating the olive oil industry. Molecular methods that allow fast, automated X. fastidiosa detection are crucial for monitoring the spread of the disease. Maxwell® RSC Instruments along with the Maxwell® RSC PureFood GMO and Authentication Kit is an established automated nucleic acid extraction method for identifying X. fastidiosa in infected plant tissue. An interlaboratory report supported by the European Union showed that the Maxwell® method achieves highly sensitive, accurate and reproducible results, with greater efficiency than traditional CTAB extraction. Learn more about Xylella fastidiosa detection and how the Maxwell® method compares with CTAB extraction in this blog. Interested in a complete qPCR kit for Xylella detection? Email us for more information Don't miss out! Stay notified of Promega events, products and news. Sign Up Need Assistance? Contact Promega Customer and Technical Support Local Contact +31 (0)71 532 42 44 Products & Resources Products Applications Custom Capabilities Resources About Promega Company Info ISO Certification Corporate Responsibility Press Releases Careers © 2021 Promega Corporation, an affiliate of Promega Benelux BV. All rights reserved. Legal and Trademarks Privacy Policy Terms and Conditions Trademarks Promotional Terms and Conditions Terms and Conditions of Sale and Services Manage Cookie Consent English – Belgium Promega's Cookie Policy Close We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy. If you are located in the EEA , the United Kingdom, or Switzerland, you can change your settings at any time by clicking Manage Cookie Consent in the footer of our website. Manage My Cookies Reject All Cookies Yes, I Accept