Products Area of Interest Resources Support Company Careers Chromium Single Cell Multiome ATAC + Gene Expression Unify the transcriptome and epigenome in every cell Simultaneously profile gene expression and open chromatin from the same cell with Chromium Single Cell Multiome ATAC + Gene Expression. Multiply your power of discovery to characterize cell types and states, and uncover gene regulatory programs. Request Pricing Introducing Multiomic Insights Resources: Product Catalog Product Sheet Grant Writing Assistance Data Spotlight Single Cell Buyer's Guide One cell, two readouts Simultaneous detection of mRNA and chromatin accessibility from the same cell. Define gene regulation Discover new gene regulatory interactions. Single cell resolution Detect rare cell populations with single cell precision. Flexible and scalable Profile hundreds to tens of thousands of cells per sample. Streamlined data analysis Explore gene expression and chromatin accessibility simultaneously with easy-to-use software. Efficient lab workflow From sample to sequencing-ready library in two days. Uncover hidden insights Multiomic profiling of the transcriptome and epigenome at single cell resolution can transform your understanding of biology. Enhance your characterization of cell types and states, and gain deeper insights into underlying gene regulatory mechanisms with two readouts from every cell. Get started with Single Cell Multiome ATAC + Gene Expression and multiply your power of discovery. What You Can Do Deeper characterization of cell types and states Characterize complex cell populations and capture cellular heterogeneity to uncover hidden insights with combined epigenomic and gene expression profiling. Leverage gene expression markers to more easily interpret epigenetic profiles. Discover new gene regulatory interactions Combine discovery of regulatory elements with gene expression to explore gene regulatory interactions driving cell differentiation, development, and disease. Make the most of your precious samples Maximize insights from your limited samples by obtaining transcriptome and open chromatin information from the same cell. Explore disease complexity Understand how gene regulatory networks are disrupted in disease. Interrogate epigenetic remodeling and mechanisms of therapeutic resistance. Proven Results Videos Watch how customers are using Chromium Single Cell Multiome ATAC + Gene Expression View all Resources Simultaneous profiling of the transcriptome and epigenome at single cell resolution Laura DeMare and Kamila Belhocine, August 2020 Deep Immune Profiling at Scale: Efficient Pipelines and TEA-seq for Simultaneous Trimodal Measurements Peter Skene and Lucas Graybuck, Allen Institute for Immunology, April 2021 Highly multimodal measurements of single cells Eleni Mimitou, PhD, New York Genome Center, April 2021 View all Our End-to-End Solution Chromium Instrument with Next GEM technology Chromium X Series Our scalable instrument Chromium Controller Our compact instrument Chromium Single Cell Multiome ATAC + Gene Expression Reagents With our reagent kits, simultaneously detect gene expression and open chromatin regions from the same single cell and generate ready-to-sequence NGS libraries. Analysis and Visualization Software Cell Ranger ARC, our analysis pipeline and Loupe Browser, our visualization software. World-Class Technical and Customer Support Our expert support team can be contacted by phone or email. Workflow 1 Prepare your sample Start with a nuclei suspension isolated from cell culture, primary cells, or fresh or frozen tissue. RESOURCES Demonstrated Protocols 2 Construct your 10x library Construct a 10x barcoded library using our reagent kits and a compatible Chromium instrument. Each member of the Chromium instrument family encapsulates each cell with a 10x barcoded Gel Bead in a single partition. Within each nanoliter-scale partition, cells undergo reverse transcription to generate cDNA, which shares a 10x Barcode with all cDNA from its individual cell of origin. RESOURCES User Guide 3 Sequence The resulting 10x barcoded single cell gene expression and ATAC-seq libraries are compatible with standard NGS short-read sequencing on Illumina sequencers, for massively parallel profiling of thousands of individual cells. RESOURCES Sequencing Requirements Sequencing Technical Note 4 Analyze Your Data Our Cell Ranger ARC analysis software generates open chromatin and gene expression profiles for each cell, identifies clusters of cells with similar profiles, and calculates linkages between open chromatin peaks and gene expression. ANALYSIS PIPELINES OUTPUT Output includes QC information and files that can be easily used for further analysis in our Loupe Browser visualization software, or third-party R or Python tools. RESOURCES Cell Ranger ARC Analysis Pipeline Interpreting the Web Summary File 5 Visualize Your Data Use our Loupe Browser visualization software to interactively explore your results, compare chromatin accessibility and gene expression across cell types and states, and investigate linkages between open chromatin peaks and gene expression. DO I NEED TO BE A BIOINFORMATICIAN TO USE IT? Loupe is a point-and-click software that’s easy for anyone to download and use. RESOURCES Loupe Visualization Software Downloadable Datasets Frequently Asked Questions What does Single Cell Multiome ATAC + Gene Expression enable you to do? How does the sensitivity of Single Cell Multiome ATAC + Gene Expression compare to the standalone Single Cell 3’ Gene Expression and Single Cell ATAC assays? Is Single Cell Multiome ATAC + Gene Expression compatible with Feature Barcode technology? What types of samples can be used? Can Single Cell Multiome ATAC + Gene Expression data be analyzed in combination with individual assays? Related Products View All Products Products Single Cell Multiome ATAC + Gene Expression View details Single Cell ATAC View details Single Cell Gene Expression View details Single Cell Immune Profiling View details Capabilities Combined transcriptome and open chromatin profiling in the same nucleus Epigenomic landscape by open chromatin profiling 3' whole transcriptome gene expression Targeted gene expression Sample multiplexing Paired full-length TCR / BCR sequencing 5’ whole transcriptome gene expression Targeted gene expression Extensions – – Sample multiplexing Cell Surface Protein CRISPR Screening Cell Surface Protein Antigen Specificity Instruments Chromium X Series Chromium Controller Chromium X Series Chromium Controller Chromium X Series Chromium Controller Chromium Connect Chromium X Series Chromium Controller Chromium Connect Get Started Request Pricing ABOUT Products Platform Company Careers Contact Employment Verification LEGAL NOTICES Privacy Policy Terms of Use Email Preferences Other Legal Notices HEADQUARTERS +1 925 401 7300 +1 800 709 1208 info@10xgenomics.com RESOURCES Publications Support Videos SOCIAL SIGN UP FOR PRODUCT AND EVENT UPDATES Subscribe © 2021 10x Genomics. 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