Texcell / Research Cell Banking / Cell Line Generation Cell Line Generation Texcell’s Cell Culture team has nearly 25 years of experience using cell-based systems for protein expression. A key factor in controlling production costs of biopharmaceutical proteins is the rapid development of recombinant cell lines that produce high levels of expressed protein products. To help facilitate achieving this goal, our team offers the following important core services to expedite the development process at the R&D level: Transfection of recombinant plasmid DNA into mammalian cell lines via lipofectamine or PEI, or transduction of a lentiviral vector Stable cell selection using appropriate selective agents (G418, hygromycin, puromycin, etc.) Single-cell plating in 96-well plate or cloning by limiting dilution Cell clone validation by ELISA or qPCR Cryopreservation of the high-level expresser cell line clones for future use Preparation of a Research Cell Bank (RCB) from selected clonal cell lines Using the above approaches, an effective selection strategy can be designed to identify monoclonal cell lines that should stably express high levels of recombinant proteins in culture; these cells will then be ideally suited for transfer to our GMP Cell Banking prior to large-scale production of proteins in standard fed-batch fermentation processes. Contact us now and get started! Biosafety Viral Clearance GMP Cell Banking Research Cell Banking Cell Line Generation Cell Line Expansion Clonal Selection Research Cell Banking Medical Devices BioAssays in vitro BioAssays in vivo Toxicology Works contracted to Texcell SA might be eligible to Research Tax Credit (Crédit Impôt Recherche). Please inquire. Texcell SA 1, rue Pierre Fontaine 91058 Evry Cedex FRANCE Texcell Asia Level 37, Tapei 101 Tower n°7 Section 5 Xinyi Rd101 Taipei, TAIWAN Vivo Science FabrikStr. 3 48599 Gronau GERMANY Texcell Japan 4F Pacific Marks Nishi-Umeda 2-6-20 Umeda, Kita-ku, Osaka 530-0001, JAPAN Texcell North America 4991 New Design Road Frederick MD 21703 USA +1 (301) 363-5600 Follow us on LinkedIn ! Mentions légales - © 2020 Texcell We'll assume you're ok with this, but you can opt-out if you wish.AcceptReject

GMP Cell Banking Biosafety Viral Clearance Medical Devices R&D Cell Banking BioAssays in vitro BioAssays in vivo Toxicology Works contracted to Texcell SA might be eligible to Research Tax Credit (Crédit Impôt Recherche). Please inquire. Texcell SA 1, rue Pierre Fontaine 91058 Evry Cedex FRANCE Texcell Asia Level 37, Tapei 101 Tower n°7 Section 5 Xinyi Rd101 Taipei, TAIWAN Vivo Science FabrikStr. 3 48599 Gronau GERMANY Texcell Japan 4F Pacific Marks Nishi-Umeda 2-6-20 Umeda, Kita-ku, Osaka 530-0001, JAPAN Texcell North America 4991 New Design Road Frederick MD 21703 USA +1 (301) 363-5600 Follow us on LinkedIn ! Mentions légales - © 2020 Texcell We'll assume you're ok with this, but you can opt-out if you wish.AcceptReject Read More