Products Selected Applications Company Service & Contact | Calculator | English HOME COHERENT ANTI-STOKES RAMAN SCATTERING Coherent Anti-Stokes Raman Scattering Overview Download Contact Coherent Anti-Stokes Raman Scattering (CARS) Coherent anti-Stokes Raman Scattering (as well as Stimulated Raman Scattering) create high imaging contrast without labeling. The technology involves two laser beams. The pump laser beam and a Stokes laser beam interact, producing an anti-Stokes signal. CARS is sensitive to the vibrational modes of samples and visualizes the vibrational contrast of molecules. The samples, even living objects, remain almost unaffected. A Coherent Anti-Stokes Raman Scattering (CARS) signal was first recorded in the mid-1960s. At that time, it was called “Three wave mixing experiment”. After ten years, first applications on biological samples were shown. CARS spectroscopy is used to get detailed information of molecules or identification of components within a sample, because of the structural fingerprint. CARS – Microscopy is an imaging method which is based on the intrinsic vibrational contrast of certain molecules and thus allows a high-resolution representation of structures. Using two laser beams with a certain difference of the two frequencies, vibrational states of molecules can be probed. To differentiate between vibrational states, one of the beams, Pump or Stokes, must be tuneable. The resulting inelastic scattering of blue-shifted photons (Anti-Stokes or CARS – Signal) are used for imaging. While fluorescence microscopy is more sensitive, CARS – Microscopy offers a higher resolution at lower power level due to the multi-photon process, without the need of complex preparation of the sample with dyes (label free) that can influence the sample and the results of the experiment. In respect to this, this method is suitable for studies on living samples (in vivo), whereby the sample remains virtually unaffected. Reference Guide In our reference guide we present more than 50 + publications for which the picoEmerald has been used to employ Stimulated Raman Scattering or Coherent anti-Stokes Raman Spectroscopy. Here it is available for Download: APEs picoEmerald Reference List Edition October 2017 Related Product CARS & SRS with picoEmerald Stimulated Raman Scattering & Coherent anti-Stokes Raman Scattering WELCOME AT APE Our repertoire encompasses a large range of nonlinear optics equipment, from spectrometers to harmonic generators, from pulse compressors to quantum-dot single photon generation sources. Over the years, APE has built up an impressive array of more than 30 products entirely developed and produced within its headquarters in Berlin. NEWS ARPES news APE turns 29 years today. Mittweidaer Lasertagung Day of Photonics Innovation Seminar 2021 CONTACT US Address: Plauener Strasse 163-165 | Haus N | 13053 Berlin | Germany Phone: +49 30 986 011-30 Email: sales@ape-berlin.de APE ON LINKEDIN Linkedin GENERAL TERMS Impressum Terms & Conditions Privacy Information © Copyright 2018/2019. All Rights Reserved. We use cookies to ensure that we give you the best experience on our website. If you continue to use this site we will assume that you are happy with it.Ok