Contact us! Main navigation HOME PRODUCT TYPES CAPABILITIES APPLICATIONS RESOURCES ABOUT BUILD-A-FILTER CONTACT PRODUCTS Filters for PCR and RT-PCR Polymerase Chain Reaction (PCR) and Reverse-Transcriptase PCR (RT-PCR) are DNA-amplification methods used in a variety of fields. They can be used to detect small amounts of target DNA in many types of samples, from biological samples, as in disease testing (like COVID-19 or strep throat) to forensic testing (fingerprints and crime scenes). Because DNA is double-stranded, each round of replication results in 2 copies of the sequence. This rapidly increases the amount of DNA in the sample. By using primers (small DNA molecules that tell an enzyme where to start replicating) that are specific to the signal of interest, only the relevant DNA is amplified. Fluorescence probes are used to determine how much relevant DNA is in the sample. The fluorescent moieties are attached to short, single-stranded DNA probes that bind to specific sequences in the DNA sample. Fluorescence can be turned on or off depending on whether the DNA probe is bound to the DNA. Fluorescence intensity is proportional to the amount of the specific DNA sequence in the sample. Nearly all fluorescence systems consist of 3 major components- an excitation light source (lamp, LED or laser), filters (to clean up the excitation wavelengths and select the emission wavelengths) and a detector. Omega has been a leader in fluorescence filters since the 1970s. A list of stock filter sets for the common PCR dyes are listed below. These are 25 mm round for excitation and emission filters and 25.7x36 mm for dichroics designed to work at 45 degrees. We also have extensive plate stock and components available, so contact us if you have special configuration requests or you don’t see what you’re looking for here. Fluorophore Ex Max (nm) Em Max (nm) Suggested Filter Set SYBR green 497 520 XF404 Cy-5 651 670 XF416 FAM 494 520 XF404 JOE 520 548 XF405, XF402 VIC 538 554 XF405, XF402 HEX 535 556 XF405, XF402 NED 546 575 XF405, XF402 PET 556 595 XF402 ROX 575 602 XF424 TAMRA 555 580 XF402 TET 521 536 XF405, XF402 Texas Red 595 615 XF424 View Products Shop for all Fluorescence Sets Additional Information Learn more about PCR and RT-PCR Use our spectral viewer- curvomatic Learn more about how fluorescence works Omega Optical, LLC 21 Omega Drive Brattleboro, VT 05301 USA U.S. Toll-Free: 1-866-488-1064 International: +1 (802) 251-7300 Sales and Support: sales@omegafilters.com Press: marketing@omegafilters.com Terms and Conditions Site Map Careers Privacy Policy Events Employee Login ITAR Registered ISO 9001:2015 Certified Made in the USA Omega cannot guarantee availability of displayed products. Contact us for details, or to request pricing of a custom part. © 2014 - 2021 Omega Optical, LLC. All Rights Reserved. By using this website, you agree to our use of cookies. We use cookies to improve the user experience and to help our website run effectively. To learn more, read our Privacy Policy. ACCEPT