Products Area of Interest Resources Support Company Careers Chromium Single Cell Gene Expression Reveal the full complexity of cellular diversity, cell by cell Chromium Single Cell Gene Expression provides single cell transcriptome 3' gene expression and multiomic capabilities to profile tens of thousands of cells. Explore cellular heterogeneity, novel targets, and biomarkers with combined gene expression, surface protein expression, or CRISPR edits in each cell. Request Pricing See How It Works Resources: Product Catalog Product Sheet Grant Writing Assistance Single Cell Buyer's Guide Single cell resolution at scale Capture the full heterogeneity of a sample, in hundreds of thousands of cells. Multiomic cellular readout Measure 3’ mRNA, cell surface proteins, CRISPR perturbations, and more. Solutions for any scale From low-throughput pilot experiments to million-cell studies, find the kit that fits your needs. Streamlined data analysis Explore and interpret single cell gene expression profiles with easy-to-use software. One-day lab workflow From sample to sequencing-ready library in one day. Diverse sample compatibility Demonstrated on fixed, fresh, and frozen samples, as well as whole cells and nuclei. Explore what you can do Characterize complex cell populations Discover novel cell types and states Unravel complex cellular processes Scalable functional genomics Flexible solutions to meet your needs The standard in single cell gene expression Chromium Single Cell Gene Expression can do it all—from handling large-scale projects to discovering the diversity of gene expression, cell by cell. Download Product Sheet Initial insights Get started with single cell gene expression—run pilot studies or optimize future experiments with an affordable solution for small-scale projects. Single Cell Gene Expression LT Multiomic analysis Simultaneously measure cell surface protein expression or CRISPR perturbations with 3’ gene expression. CRISPR ScreeningCell Surface Protein Large-scale projects Tackle your most ambitious projects, from drug screening to pre-clinical trials, with complementary solutions for larger-scale projects. Single Cell Gene Expression HTTargeted Gene Expression Sample Multiplexing with 3' CellPlex Workflow automation Maximize lab productivity and obtain consistent, reproducible results with Single Cell Gene Expression for Chromium Connect. Automated Single Cell Gene Expression Proven Results Videos Watch how customers are using Chromium Single Cell Gene Expression. View all Publications Resources Getting Started with Single Cell Gene Expression Jens Durruthy Durruthy, Ph.D, March 2019 Tumor Dissociation for Single Cell RNA-seq Jill Herschleb and Sharmila Chatterjee, June 2018 Scale your single cell studies with sample multiplexing Jason Koth and Elliott Meer, January 2021 View all Our end-to-end solution Chromium instruments with Next GEM technology Chromium X Series Our scalable instrument Chromium Controller Our compact instrument Chromium Connect Our automated instrument Chromium Single Cell Gene Expression reagents With our reagent kits, explore cellular diversity through 3’ mRNA, cell surface proteins, CRISPR perturbations, and more. Analysis and visualization software Cloud Analysis Our data processing platform Cell Ranger Our analysis pipelines Loupe Browser Our visualization software World-class technical and customer support Our expert support team can be contacted by phone or email. Workflow Scroll to the right to see the base workflow for Single Cell Gene Expression. If you’re using 3’ CellPlex or our Feature Barcode Kit for CRISPR Screening or Cell Surface Protein, we provide additional protocols for preparing your samples. 1 Prepare your sample Start with hundreds to hundreds of thousands of cells. Follow best practices for washing, counting, and concentrating cells to minimize the presence of cellular aggregates, and retrieve high-quality single cell suspensions. RESOURCES Single Cell Preparation Guide Demonstrated Protocols 2 Construct your 10x library Construct a 10x barcoded library using our reagent kits and a compatible Chromium instrument. Each member of the Chromium instrument family encapsulates each cell with a 10x barcoded Gel Bead in a single partition. Within each nanoliter-scale partition, cells undergo reverse transcription to generate cDNA, which shares a 10x Barcode with all cDNA from its individual cell of origin. RESOURCES User Guide / Protocol LT User Guide / Protocol HT User Guide / Protocol Automated User Guide / Protocol 3 Sequence The resulting 10x barcoded library is compatible with standard NGS short-read sequencing on Illumina sequencers, for massive transcriptional profiling of thousands of individual cells. RESOURCES Sequencer Compatibility List 4 Analyze your data Our Cell Ranger analysis software generates expression profiles for each cell, identifies clusters of cells with similar profiles, and aggregates data from multiple samples. ANALYSIS PIPELINES OUTPUT Output includes QC information and files that can be easily used for further analysis in our Loupe visualization software, or third-party R or Python tools. RESOURCES Cell Ranger Analysis Pipelines Running Cell Ranger in Cloud Analysis 5 Visualize your data Use our Loupe Browser visualization software to interactively explore your results, study expression patterns for genes of interest, determine cell types, and perform comparative analysis between clusters and samples. DO I NEED TO BE A BIOINFORMATICIAN? Loupe is a point-and-click software that’s easy for anyone to download and use. RESOURCES Loupe Browser Visualization Software Downloadable Datasets Frequently asked questions What does Chromium Single Cell Gene Expression enable you to do? What RNA types can be profiled with the solution? What types of samples are compatible? How do I analyze my single cell data? Is there a cell size limit? Related Products View All Products Products Single Cell Gene Expression View details Single Cell Immune Profiling View details Spatial Gene Expression View details Single Cell ATAC View details Capabilities 3' whole transcriptome gene expression Targeted gene expression Sample multiplexing Paired full-length TCR / BCR sequencing 5’ whole transcriptome gene expression Targeted gene expression 3' whole transcriptome gene expression Targeted gene expression Compatible with FFPE and fresh frozen tissue sections Epigenomic landscape by open chromatin profiling Extensions Sample multiplexing Cell Surface Protein CRISPR Screening Cell Surface Protein Antigen Specificity Spatial Gene Expression with Immunofluorescence Immunofluorescence protein co-detection – Instruments Chromium X Series Chromium Controller Chromium Connect Chromium X Series Chromium Controller Chromium Connect – Chromium X Series Chromium Controller Get Started Request Pricing ABOUT Products Platform Company Careers Contact Employment Verification LEGAL NOTICES Privacy Policy Terms of Use Email Preferences Other Legal Notices HEADQUARTERS +1 925 401 7300 +1 800 709 1208 info@10xgenomics.com RESOURCES Publications Support Videos SOCIAL SIGN UP FOR PRODUCT AND EVENT UPDATES Subscribe © 2021 10x Genomics. 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