Products Selected Applications Company Service & Contact | Calculator | English HOME CARS & SRS WITH PICOEMERALD CARS & SRS with picoEmerald Stimulated Raman Scattering & Coherent anti-Stokes Raman Scattering More general information about the tuneable picosecond laser picoEmerald can be found here. Overview Specification Download Contact Stimulated Raman Scattering (SRS) Stimulated Raman Scattering (SRS) microscopy for non-invasive label-free imaging is facilitated via the picoEmerald system. SRS microscopy provides almost background-free imaging contrast. It allows simple spectroscopic identification of the samples’ fingerprint region based on Raman spectra databases. Switching over to SRS microscopy is relatively straightforward for researchers who already have CARS experience. Coherent Anti-Stokes Raman Spectroscopy (CARS) CARS Coherent anti-Stokes Raman Spectroscopy (as well as Stimulated Raman Scattering) create high imaging contrast without labeling. The technology involves two laser beams. CARS is sensitive to the vibrational modes of samples and visualizes the vibrational contrast of molecules. The samples, even living objects, remain almost unaffected. SRS Microscopy and CARS Spectroscopy with a Tunable ps Two-Color Laser CARS & SRS in a Single-box SRS Stimulated Raman Scattering microscopy and CARS Coherent Anti-Stokes Raman Spectroscopy have migrated from physics labs to life-science labs, so APE has responded with an easy-to-use and truly hands-off SRS and CARS light source: picoEmerald. In a single-box, picoEmerald provides fully automated temporal- and spatial-overlapping ultra-short pulse trains: 1032 nm from the laser oscillator, plus the OPO Signal beam, and OPO Idler beam. Turning Complexity into a Turn-Key System The light source is the most crucial and complex component of a SRS or CARS microscopy system. Proper setup and calibration are essential for accurate and reproducible imaging. picoEmerald is a turn-key system combining user-friendly automation features and the utility of open-architecture light sources in a single box. Applications Stimulated Raman Spectroscopy (SRS) Coherent Anti-Stokes Raman Spectroscopy (CARS) SRS Microscopy of Vibrational Probes Second Harmonics Imaging (SHG) Pump-Probe Spectroscopy Surface Enhanced Hyper Raman Spectroscopy (SEHRS) Setup and Use with Microscopes picoEmerald is compatible with nearly all popular confocal microscope systems. The current publication list names at least those configurations: Home-built setups Leica SP-Series Olympus FV-Series Nikon Ti-U Platform Thorlabs Multiphoton Zeiss LSM-Series Horiba By integrating SRS and CARS technology into Leica’s confocal SP5/SP8 microscopy system, Leica Microsystems has tightly integrated the picoEmerald into their LAS AF system software. Similary tight software integration is also available with Thorlabs’ multi-photon microscopes. Software integration into other third-party microscopes or home-built setups is also supported. Ethernet TCP/IP and serial RS232 interfaces are available with picoEmerald for this purpose. APE’s user-friendly automation combined with a graphical user interface (GUI) also makes it convenient when using picoEmerald and microscope as independent instruments, without the need for software integration. Introduction to Stimulated Raman Stimulated Raman Scattering (SRS) is based on the effect that excited molecules emit spontaneous Raman scattering, SRS is a further development of CARS. In the event of two different laser beams with a frequency difference which matches the vibrational frequency, the Raman signal will be amplified. So this process is not spontaneously anymore, it is stimulated. This technique requires two laser beams. A Pump and a Stokes beam. One of these beams must be tuneable in order to meet the difference of the frequencies, so that the molecules can be excited into the virtual state and to bring it to the vibrational state. As at CARS, the molecules are only excited if the frequency coincides with the vibrational resonance. The tuneable Pump beam gets a loss in intensity after excitation of the molecules (stimulated Raman loss, SRL). On the opposite, the intensity of the non-tuneable Stokes beam increases (stimulated Raman gain – SRG). The different intensities between input Pump beam and output Pump beam are very low. So low, that the true measurement signal is lost in the noise. To measure this difference, the lock-in technique is used. For this the Stokes beam is modulated to a fixed frequency by an Electro-Optical Modulator (EOM). APE has integrated such EOM into picoEmerald. Unlike CARS, SRS provides results with no non-resonant background. The assignments of CARS spectra are more complicated and thus the interpretation is more susceptible to errors. SRS is based on spontaneously Raman scattering and so the obtained SRS spectra are almost identical with spectra of spontaneously Raman scattering. Furthermore, SRS signal is directly linearly dependent to the concentration. Turning Complexity into a Turn-Key System The light source is the most crucial and complex component of a CARS Coherent Anti-Stokes Raman Spectroscopy or SRS Stimulated Raman Scattering system. Proper setup and calibration are essential for accurate and reproducible imaging. picoEmerald is a turn-key system combining user-friendly automation features and the utility of open-architecture light sources in a single box. WELCOME AT APE Our repertoire encompasses a large range of nonlinear optics equipment, from spectrometers to harmonic generators, from pulse compressors to quantum-dot single photon generation sources. Over the years, APE has built up an impressive array of more than 30 products entirely developed and produced within its headquarters in Berlin. NEWS ARPES news APE turns 29 years today. Mittweidaer Lasertagung Day of Photonics Innovation Seminar 2021 CONTACT US Address: Plauener Strasse 163-165 | Haus N | 13053 Berlin | Germany Phone: +49 30 986 011-30 Email: sales@ape-berlin.de APE ON LINKEDIN Linkedin GENERAL TERMS Impressum Terms & Conditions Privacy Information © Copyright 2018/2019. All Rights Reserved. We use cookies to ensure that we give you the best experience on our website. If you continue to use this site we will assume that you are happy with it.Ok