PRODUCTS CONTACT COMPANY EVENTS ACADEMY NEWS What is flow cytometry? Home/Academy/Cytognos technology/Flow Cytometry/What is flow cytometry? Chronic Lymphoproliferative Disorders Acute Leukemia Plasma Cell Dyscrasias Primary Immunodeficiencies Monocyte subsets monitoring Cytognos technology EuroFlow™ Flow Cytometry What is flow cytometry? What is an antibody? Applications of flow cytometry The flow cytometer Software Flow Cytometry What is flow cytometry? Last update: October 1st, 2019 Cytometry is a process in which physical and/or chemical characteristics of single cells, of other biological or nonbiological particles, are measured. In flow cytometry, the measurements are made simultaneously as the cells or particles, suspended in fluid stream, pass individually through a beam of light of a measuring device, the flow cytometer. Some flow cytometers can physically separate cell subsets (sorting) based on their cytometric characteristics (cell sorters). Flow cytometry can be used to count large numbers of cells or particles based on size, internal complexity, phenotype, cellular state, cell function, DNA content, gene expression, and to quantify these same cellular properties at a single-cell level. This allows to evaluate: Whether cells are present in a specimen How many are there What kinds of cells are they What their functional characteristics might be OPTICAL MICROSCOPY FLOW CYTOMETRY Its working depends on the light scattering features of the cells under investigation, which may be derived from dyes or monoclonal antibodies targeting either extracellular molecules located on the surface or intracellular molecules inside the cell (see Flow Cytometry probes). This approach makes flow cytometry a powerful tool for detailed analysis of complex populations in a short period of time. Resources Publications: Adan A, et al. Flow cytometry: basic principles and applications. Crit Rev Biotechnol. 2017 Mar;37(2):163-176. Go to publication Cossarizza A, et al. Guidelines for the use of flow cytometry and cell sorting in immunological studies. Eur J Immunol. 2017 Oct;47(10):1584-1797. Go to publication Piyasena ME, et al. Multinode acoustic focusing for parallel flow cytometry. Anal Chem. 2012 Feb; 84(4): 1831–1839. Go to publication Ward M, et al. Fundamentals of Acoustic Cytometry. Curr. Protoc. Cytom. 49:1.22.1-1.22.12. Go to publication Brown M and Wittwer C. Flow Cytometry: Principles and Clinical Applications in Hematology. Clinical Chemistry. 2000 April;46:8(B)1221–1229. Go to publication Howard M. Shapiro Practical flow cytometry, 4th ed. John Wiley hi Sons, Inc. 2003 August; 736 Pages. Go to publication Our products Next Generation Flow Solutions Acute Leukemia Chronic Lymphoproliferative Disorders Plasma Cell Dyscrasias Primary Immunodeficiencies Omnicyt™ Infinicyt™ The company About us Contact & Support Events Work with us Projects Subscribe to Newsletter Useful information EuroFlow™ Cytognos technology Minimal Residual Disease Clinical trials Scheduled webinars FAQs Legal Notice Legal Notice Terms of use Privacy Policy Cookie Policy Terms and Conditions of Sale Anti-corruption Policy License Agreement Cytognos, S.L. complies with ISO 9001:2015 Quality Management and ISO 13485:2016 for the design, development, production and sale of antibodies and flow cytometry kits for In Vitro products and research. Cytognos, S.L. products for In Vitro Diagnostic comply with Directive 98/79/EC. Copyright © 2021 Cytognos S.L.. All Rights Reserved. All products listed are labeled with some regulatory status as per indicated below: “RUO” or “For Research Use Only”: not intended for diagnostic or therapeutic procedures. “CE-IVD”: products intended for in vitro diagnostic use and conforming to European Directive (98/79/EC). “LUO” or “Laboratory Use Only”: general purpose laboratory reagents. “ASR” or “Analyte Specific Reagent”: reagents in which the analytical and performance characteristics are not established. ASRs are regulated by the US Food and Drug Administration (FDA). Cookies We use cookies to improve our website and your experience when using it. Cookies used for the essential operation of the site have already been set. To find out more about the cookies we use and how to delete, please read more. Accept Read more Change Settings