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Blog About About Tecan Who we are Mission, Vision and Values Management Board of Directors History Press center overview News archive Quality policy Investors Investors overview Stock quotes Annual reports Press Center Press center overview News archive Annual reports Careers Open positions Tecan Journal Tecan Journal Support Contact Life Sciences Diagnostics Partnering Tecan Group global Select language Products Liquid handling & automation Fluent® Overview Cell-based assays Compound Genomics FluentControl™ Literature Freedom EVO® series Overview Base Units Freedom EVO 75 base unit Freedom EVO 100 base unit Freedom EVO 150 base unit Freedom EVO 200 base unit Liquid handling arms Single channel LiHa Dual channel Liha 8 Plus 1 Access Liquid Liha Air Liha MultiChannel Arm™ (MCA 96) MultiChannel Arm™ (MCA 384) Robotic arms Robotic Manipulator (RoMa) Arm Extended Robotic Manipulator (RoMa) Arm Pick and Place (PnP) Arm Options Te-Shake™ Te-VacS™ Te-MagS™ MIO™ PosID™ Te-PS™ Te-Stack™ Carousel Technology Process security Software Freedom EVOware® Tecan D300e digital dispenser Overview Software Videos Literature Modules & options overview Robotic arms Air LiHa and liquid LiHa - best of both worlds Air liquid handling arm (LiHa) Liquid handling arm (LiHa) 8 Plus 1 access liquid handling arm MultiChannel Arm™ 96 (MCA 96) MultiChannel Arm™ 384 (MCA384) Robotic manipulator arm (RoMa) Pick and place (tube handling arm) Liquid handling arm options Fixed tips Disposable tips (DiTis) Pressure monitored pipetting Tip positioning system (Te-PS) Labware logistics Active worktable Stacking device Interlink option for multiple instruments Labware carriers Barcode sample identification Positive identification system Incubation devices Monitored incubator option Shakers/mixers/vortexers Te-Shake™ Plate readers & washers Washer and blot processor Microplate reader Separation devices Vacuum separation module (Te-Vacs™) Magnetic bead separation module (Te-MagS™) Multi dispensers DynamicFill™ technology (Te-Fill) Others ADME devices (pION) Cell transfection Nucleofector® Tecan Integration Group (TIG) Overview Insights Latest projects (TIG Flyer) Cell biology Protein science Genomics HTS - TRAC Sample preparation Chemistry Parts Others Technologies Microplate readers Infinite® F50 and Infinite F50 Robotic Technology Software Add-ons and Tools Literature Specifications Infinite® 200 PRO Read modes Software Accessories Scientific citations Infinite 200 PRO Configurations Lumi M Nano M Nano Plus F Nano Plus M Plex F Plex Upgrades Spark® Overview Configurations Technology Software Accessories Spark-Stack™ plate stacker Literature Webinars and Videos Spark read modes Absorbance Fluorescence Luminescence Cell counting & imaging Live cell imaging Live viewer Cell counting and viability Live-cell imaging and confluence Live-cell package Specification Sunrise™ Technology Software Add-ons and Tools Literature Specification Microplate washers HydroFlex™ Features Options Applications Software Specifications Videos Literature HydroSpeed™ Features Options Applications Software Specifications Videos Literature Microsite Software Freedom EVOware® Specifications Literature Related topics TouchTools™ Instant Pipetting™ CNS and EVO remote Process Security Extensibility Dynamic scheduling Labware logistics Recovery User management EVO Remote and CNS Nucleic acid purification wizard Video Literature Related topics TouchTools™ Nucleic acid extraction PCR wizard TouchTools™ Video Literature Related topics Freedom EVOware® Instant Pipetting™ wizard Nucleic acid purification wizard PCR wizard Instant Pipetting™ Related topics Freedom EVOware® TouchTools Suite™ CNS and EVO remote Freedom EVOware® sample tracking Features Specifications Literature Freedom EVOware® normalization wizard Specifications Literature Freedom EVOware® hit-picking Specifications i-control™ software Literature Magellan™ data analysis software Features Specifications Videos Literature XFluor™ Consumables Disposable Tips Liquid handling disposable tips Shop MCA 96 disposable tips Shop MCA 384 disposable tips Shop Labware Reagent troughs Microplates Shop Functional consumables D300e Dispensehead Cassettes QC Kit Shop AC Extraction Plate Shop Immunoassays & antibodies Applications Genomics Nucleic acid purification Quantification and normalization PCR set-up NGS sample preparation Biobanking Synthetic biology Drug discovery Target identification and validation Genomics Proteins Cell biology Screening and lead optimization Assay set-up and analysis Screening-cell-based and biochemical Immunoassays Protein characterization Literature Compound management Compound dissolution Library maintenance Assay plate preparation Literature ADME-Tox Permeability Solubility Metabolism Protein binding Lead characterization Literature Analytical chemistry Dilute and shoot Liquid-liquid extraction Cell biology Cloning and transfection Cell culture Cell-based assay Cellular imaging and analysis Technical literature / downloads Protein science Protein purification and bioprocessing Sample preparation for mass spectrometry Protein detection and analysis Crystallography Applied markets Agriculture Veterinary Food Forensic solutions Freedom EVO® with DNA IQ Performance data User interface Freedom EVO configuration Installation process Technical literature / download Freedom EVOware® normalization wizard Applications Specifications Technical literature / download ELISA solutions Sample preparation for Luminex® assays on Freedom EVO® Technical literature / download Case studies Integrated BioBank of Luxembourg Repsol Aqsen Blog About About Tecan Who we are Mission, Vision and Values Management Board of Directors History Press center overview News archive Quality policy Tecan Journal Investors Investors overview Stock quotes Annual reports Press Center Press center overview News archive Annual reports Careers - Open positions Support Contact Select language Life Sciences Products Liquid handling & automation Fluent® Overview Cell-based assays Compound Genomics FluentControl™ Literature Freedom EVO® series Overview Base Units Freedom EVO 75 base unit Freedom EVO 100 base unit Freedom EVO 150 base unit Freedom EVO 200 base unit Liquid handling arms Single channel LiHa Dual channel Liha 8 Plus 1 Access Liquid Liha Air Liha MultiChannel Arm™ (MCA 96) MultiChannel Arm™ (MCA 384) Robotic arms Robotic Manipulator (RoMa) Arm Extended Robotic Manipulator (RoMa) Arm Pick and Place (PnP) Arm Options Te-Shake™ Te-VacS™ Te-MagS™ MIO™ PosID™ Te-PS™ Te-Stack™ Carousel Technology Process security Software Freedom EVOware® Tecan D300e digital dispenser Overview Software Videos Literature Modules & options overview Robotic arms Air LiHa and liquid LiHa - best of both worlds Air liquid handling arm (LiHa) Liquid handling arm (LiHa) 8 Plus 1 access liquid handling arm MultiChannel Arm™ 96 (MCA 96) MultiChannel Arm™ 384 (MCA384) Robotic manipulator arm (RoMa) Pick and place (tube handling arm) Liquid handling arm options Fixed tips Disposable tips (DiTis) Pressure monitored pipetting Tip positioning system (Te-PS) Labware logistics Active worktable Stacking device Interlink option for multiple instruments Labware carriers Barcode sample identification Positive identification system Incubation devices Monitored incubator option Shakers/mixers/vortexers Te-Shake™ Plate readers & washers Washer and blot processor Microplate reader Separation devices Vacuum separation module (Te-Vacs™) Magnetic bead separation module (Te-MagS™) Multi dispensers DynamicFill™ technology (Te-Fill) Others ADME devices (pION) Cell transfection Nucleofector® Tecan Integration Group (TIG) Overview Insights Latest projects (TIG Flyer) Cell biology Protein science Genomics HTS - TRAC Sample preparation Chemistry Parts Others Technologies Microplate readers Infinite® F50 and Infinite F50 Robotic Technology Software Add-ons and Tools Literature Specifications Infinite® 200 PRO Read modes Software Accessories Scientific citations Infinite 200 PRO Configurations Lumi M Nano M Nano Plus F Nano Plus M Plex F Plex Upgrades Spark® Overview Configurations Technology Software Accessories Spark-Stack™ plate stacker Literature Webinars and Videos Spark read modes Absorbance Fluorescence Luminescence Cell counting & imaging Live cell imaging Live viewer Cell counting and viability Live-cell imaging and confluence Live-cell package Specification Sunrise™ Technology Software Add-ons and Tools Literature Specification Microplate washers HydroFlex™ Features Options Applications Software Specifications Videos Literature HydroSpeed™ Features Options Applications Software Specifications Videos Literature Microsite Software Freedom EVOware® Specifications Literature Related topics TouchTools™ Instant Pipetting™ CNS and EVO remote Process Security Extensibility Dynamic scheduling Labware logistics Recovery User management EVO Remote and CNS Nucleic acid purification wizard Video Literature Related topics TouchTools™ Nucleic acid extraction PCR wizard TouchTools™ Video Literature Related topics Freedom EVOware® Instant Pipetting™ wizard Nucleic acid purification wizard PCR wizard Instant Pipetting™ Related topics Freedom EVOware® TouchTools Suite™ CNS and EVO remote Freedom EVOware® sample tracking Features Specifications Literature Freedom EVOware® normalization wizard Specifications Literature Freedom EVOware® hit-picking Specifications i-control™ software Literature Magellan™ data analysis software Features Specifications Videos Literature XFluor™ Consumables Disposable Tips Liquid handling disposable tips Shop MCA 96 disposable tips Shop MCA 384 disposable tips Shop Labware Reagent troughs Microplates Shop Functional consumables D300e Dispensehead Cassettes QC Kit Shop AC Extraction Plate Shop Immunoassays & antibodies Applications Genomics Nucleic acid purification Quantification and normalization PCR set-up NGS sample preparation Biobanking Synthetic biology Drug discovery Target identification and validation Genomics Proteins Cell biology Screening and lead optimization Assay set-up and analysis Screening-cell-based and biochemical Immunoassays Protein characterization Literature Compound management Compound dissolution Library maintenance Assay plate preparation Literature ADME-Tox Permeability Solubility Metabolism Protein binding Lead characterization Literature Analytical chemistry Dilute and shoot Liquid-liquid extraction Cell biology Cloning and transfection Cell culture Cell-based assay Cellular imaging and analysis Technical literature / downloads Protein science Protein purification and bioprocessing Sample preparation for mass spectrometry Protein detection and analysis Crystallography Applied markets Agriculture Veterinary Food Forensic solutions Freedom EVO® with DNA IQ Performance data User interface Freedom EVO configuration Installation process Technical literature / download Freedom EVOware® normalization wizard Applications Specifications Technical literature / download ELISA solutions Sample preparation for Luminex® assays on Freedom EVO® Technical literature / download Case Studies Integrated BioBank of Luxembourg Repsol Aqsen Diagnostics Products Clinical automation solutions MS sample preparation Positive pressure workstations Smart extraction consumables Dilute and shoot Liquid-liquid extraction Which smart sample prep solution best fits your application? Liquid handling & robotics Freedom EVO® series Freedom EVOlyzer® Specifications Software Technical literature/ downloads Freedom EVO® Clinical Specifications Technical literature/ downloads Clinical automation solutions Technologies Process security technologies Microplate readers Infinite® F50 and Infinite F50 Robotic Technology Software Add-ons and Tools Literature Specifications Infinite® 200 PRO Read modes Software Accessories Scientific citations Infinite® 200 PRO Configuration Lumi M Nano M Nano Plus F Nano Plus M Plex F Plex Upgrades Sunrise™ Technology Software Add-ons and Tools Literature Specifications Microplate washers HydroFlex™ Features Options Applications Software Specifications Videos Technical literature/ downloads HydroSpeed™ Features Options Applications Software Specifications Videos Technical literature/ downloads Microsite ProfiBlot™ 48 Western blot analyzer Specifications Technical literature/ downloads ProfiBlot™ T48 Specifications Technical literature/ downloads Microarray products Software Freedom EVOware® Specifications Technical literature/ downloads Related topics TouchTools™ Instant Pipetting™ CNS and EVO remote Process security Extensibility Dynamic scheduling Labware logistics Recovery User management EVO Logic™ Specifications TouchTools™ Video Technical literature/ downloads Related topics Freedom EVOware® Instant Pipetting™ wizard Nucleic acid purification wizard Instant Pipetting™ Related topics Freedom EVOware® TouchTools Suite™ CNS and EVO remote Freedom EVOware® sample tracking Features Specifications Technical Literature / Download Freedom EVOlution™ Technical literature/ downloads i-control™ software Technical literature/ downloads Magellan™ data analysis software Features Specifications Videos XFluor™ Consumables Immunoassays & antibodies Applications Molecular diagnostics Analytical chemistry Positive pressure workstations Smart extraction consumables Dilute and shoot Liquid-liquid extraction Pre- and post-analytical processing Immunodiagnostics Semi-automated immunoassay workflows Integrated immunoassay solutions Absorbance integrated Chemiluminescence integrated Software Literature Pre- and post-analytical processing Pre- and post-analytical processing Molecular diagnostics Workflows Immunoassay solutions Clinical genomics Molecular diagnostics Analytical chemistry Quality & regulatory Related products Freedom EVO® Freedom EVOlyzer® Freedom EVO® clinical Hybridization & microarrays Consumables Related topics Immunoassays & antibodies Multiplexing solutions with Luminex® Automated Immunoassays – ready to use Case Studies Munich Leukemia Laboratory Medical Laboratory Bremen Vitamin testing Partnering About Partnering Tecan advantage Partnering events Partners Case studies Siemens Healthineers Dako Omnis DaAn gene DA3000 Zivak Technologies Products & Services Customized development Concepting & design Our concept team Application expertise Risk management Platform developments Xantus Freedom EVO® series System developments Customized development Bringing it all together Tailored for your platform Liquid handling components Robotic components Cavro Omni Flex Cavro Mini Sample Processor (MSP) Cavro Omni Robot Cavro Robotic Sample Processor (RSP) Pump technologies Air Displacement Pipettor Cavro Air Displacement Pipettor (ADP) Syringe Pumps Cavro Centris Pump Cavro XLP 6000 Pump Cavro XCalibur Pump Cavro XMP 6000 Pump Cavro XE 1000 Pump Piston pumps Cavro Pulssar PBC Pump Cavro Pulssar PSR Pump Diaphragm Pumps Cavro MiniWash Pump (MW) Peristaltic Pumps Cavro Smart Peristaltic Pump (SP) Valves Smart valve Other valves Software Cavro Express Software Cavro Fusion Software Cavro integration kit Liquid handling modules Xantus modules Shaker Barcode reader Consumables & Spare parts Consumables Cavro disposable tips Shop Spare parts Syringes Valves Probes Racks Linear Option Board Smart Input/Output Board Partner services Manufacturing & support Contract manufacturing Service and support Co-marketing OEM Te-Care Helpdesk / Product Phase-outs Quality and regulatory Quality control and regulatory compliance Applications Histology - Cytology Molecular Diagnostics Applied markets Tecan Group Global Microplate readers Infinite® 200 PRO Infinite® 200 PRO Six configurations. Infinite possibilities. The Infinite 200 PRO is an easy-to-use multimode plate reader family that offers affordable, high performance detection solutions, referenced in more than 1800 peer reviewed publications. Based on this customer experience and feedback, the Infinite 200 PRO microplate plate reader has been continuously engineered for excellence. This proven platform has now evolved into a budget-friendly reader family consisting of six application-focused configurations, delivering performance and value to every lab, every day, for every assay. Overview Request pricing Talk to an expert Overview Read modes Software Accessories Scientific Citations Request pricing Talk to an expert Read modes Overview Read modes Software Accessories Scientific Citations Request pricing Talk to an expert Dedicated to delivering results for every assay, every day. Six application-focused configurations There are now six application-tailored configurations for the acclaimed and proven Infinite 200 PRO plate reader, including new options such as automated pathlength correction. This allows you to choose the perfect reader for your specific needs and budget with a fast delivery time. Learn more Easily upgradeable As instrument requirements regularly change according to ongoing research needs, each of the six configurations can be upgraded to a full multimode monochromator- and filter-based reader. This ensures your Infinite reader will deliver high quality results for every assay, every day. Learn more Infinite 200 PRO capabilities Absorbance Absorbance spectra Fluorescence (top and bottom) Fluorescence spectra (top and bottom) Time-resolved fluorescence (TRF) FRET TR-FRET Fluorescence polarization (FP) Luminescence (glow, flash, multicolor) Automated pathlength correction Absorbance cuvette port NanoQuant Plate™ Temperature control (RT +5°C to 42°C) Plate shaking (linear and orbital) Te-Inject™ reagent injectors QC tools for IQOQ services Connect™ microplate stacker ELISA DNA/RNA quantification Nucleic acid labeling efficiency Protein quantification ATP quantification Ca2+ detection HTRF®, DELFIA®, LanthaScreen® Transcreener® PolarScreen® GeneBLAzer® DLR® BRET (including NanoBRET™) Are you ready for Infinite delivery of results? Absorbance Superior performance and free wavelength choice - from ELISAs to low sample volume quantifications. Read more Fluorescence Choose between the unparalleled sensitivity of filters and the ultimate flexibility of monochromators. Read more Luminescence Optimized for glow-, flash- and multicolor-applications performed in 384-well plates. Read more Dedicated to delivering results for every assay, every day. The Infinite 200 PRO gives you a choice of monochromator- or filter-based optics. Monochromators offer you free wavelength selection and enable spectral scanning in both absorbance and fluorescence modes. Filter-based devices provide increased sensitivity and access to high-end detection technologies, such as TR-FRET and fluorescence polarization. Whichever you choose, you can be assured of high quality results from an affordable package. Despite its compact size, the Infinite 200 PRO is packed with features that set it apart. Its absorbance components are designed to offer superior performance for critical applications, such as A260/280 measurements, in a wide variety of microplates, with easy path length correction via the i-control™ software to improve data quality. The luminescence module is also completely separate from the fluorescence and absorbance optics, ensuring superb performance for glow, flash and dual-color luminescence applications. These are just two examples of how the Infinite 200 PRO is engineered to address every detail and deliver high quality results. Absorbance Superior performance and free wavelength choice – from ELISAs to low volume quantification of nucleic acids and proteins. The Infinite 200 PRO provides accurate and highly reproducible absorbance measurement capabilities, over a spectral range of 230 to 1000 nm. The absorbance module offers the flexibility to work with high or low volume samples, without compromising on your data quality. Whether you choose filter- or monochromator-based optics, you can be assured that the system is designed to tackle any absorbance assay with ease. Show more Affordable sensitivity: filter-based optics The Infinite F200 PRO’s filter-based absorbance module is the economical choice for standard absorbance applications, such as ELISAs and protein quantification assays (eg. BCA). A selection of application-specific absorbance filters are available, and the instrument’s filter slide allows you to load four absorbance filters at a time. Unmatched flexibility: monochromator-based optics Wavelength accuracy and precision are essential for an accurate and error-free nucleic acid quantification and purity checks (eg. A260/280 measurements). The Quad4 Monochromators of the Infinite M200 PRO offer industry-leading 260 nm wavelength accuracy (typically <0.5 %) and precision (typically <0.2 %) and, together with the NanoQuant Plate, provide a typical dsDNA detection limit of ≤1 ng/µl with an A260/280 ratio of over 1.8. NanoQuant Plate The patented NanoQuant Plate was the first tool specifically developed for absorbance-based, small volume nucleic acid analyses in a multimode reader. It is useful for a broad range of applications, such as DNA or RNA quantification, nucleic acid purity assessment and labeling efficiency determination. The NanoQuant Plate can simultaneously measure up to 16 samples, with sample volumes of as little as 2 µl. Quartz micro-spots are shaped to function as individual lenses, guaranteeing the best possible light transmission through each sample. Closing the plate lid effectively creates 16 quartz micro-cuvettes with a fixed path length of 0.5 mm – perfect for 230, 260 and 280 nm measurements. Samples can be easily applied using single or eight-channel pipettes, and each micro-spot can be quickly and easily cleaned to minimize the risk of cross-contamination. For convenience and optimal data quality, the NanoQuant Plate is the only low volume plate on the market that is 100 % calibration free, maintaining reliable performance while minimizing demands on your time. This innovative tool is truly a prerequisite for sensitive, reliable and easy DNA/RNA quantification in your laboratory. The system’s i-control software offers a preconfigured ‘one-click’ method for nucleic acid quantification, giving you quick, easy and reliable results at the touch of a button. Show more Additional functions Unique ways to improve absorbance results and functionality. Path length correction: Well-to-well variations in path length can be a major issue for absorbance measurements in microplates, so i-control offers easy path length correction, giving you full control for comparable readouts every time. Cuvette port: For larger sample volumes, the Infinite 200 PRO’s integrated cuvette port allows highly accurate and precise absorbance measurements, eliminating the need for a standalone cuvette spectrophotometer. Show more Zoom Key absorbance applications DNA/RNA quantification and purity checks (A260/280) Protein quantification (eg. BCA, Bradford, Lowry) ELISAs 600 nm growth curves (bacteria, yeast) Enzyme kinetics Compound characterization Literature Automating ELISAs on Tecan’s Freedom EVO® using Optimiser™ technology from Siloam Biosciences Siloam Biosciences’ OptiMax microplate (based on the Optimiser technology platform) offers rapid, sensitive and specific chemifluorescence-based ELISA procedures using exceedingly small sample volumes. The speed, sensitivity and small sample requirements are achieved as a result of the unique microfluidic design of the Optimiser technology. Protein quantification: BCA™, Modified Lowry and Bradford assays In this note we describe the use of Tecan’s Infinite F200 PRO and Infinite M200 PRO instruments for easy and sensitive protein quantification using different absorbance-based assays. Automated solution for monitoring growth of Staphylococcus aureus This application note describes the detection of S. aureus growth using Tecan’s Infinite 200 PRO microplate reader to perform 600 nm absorbance measurements. The influence of assay parameter and instrument settings on data quality is discussed. Low Volume DNA and RNA Quantification - NanoQuant The NanoQuant Plate from Tecan allows researchers to measure the concentration and purity of nucleic acids of up to 16 samples simultaneously. Additionally, the efficiency of the nucleic acid labeling with dyes can be easily determined with this tool. RNA quantification: Sorted Mouse Keratinocyte Stem Cells at Karolinska institute The isolation of adult stem cells results in very low amounts of cells due to their rare incidence in already formed tissues and organs. Here we describe the preparation and evaluation of RNA samples from a novel population of mouse keratinocyte stem cells marked by Lgr5+ expression. We used tools specifically designed for handling and measuring low RNA quantities including RNA purification and the subsequent quantification with the Infinite M200 PRO. Protein Quantification in Small-Volume Samples Tecan´s NanoQuant detection technology has been developed to facilitate the quantification of biomolecules in small-volume samples. The present note describes the implementation of the NanoQuant Plate for protein measurements with regard to essential assay parameters such as linearity, uniformity, and reproducibility. Optical pathlength correction for absorbance measurements One of the most used methods for determining nucleic acid concentrations is based on measuring the optical density of a sample at 260 nm (OD260). According to the Lambert-Beer law, the amount of absorbed light is proportional to the concentration of the sample, and to the pathlength the light has to pass through when going through the sample. Fluorescence Unparalleled sensitivity or ultimate flexibility – the choice is yours. Fluorescence signal detection in microplates is at the heart of life sciences research. The Infinite 200 PRO is available with filter- or monochromator-based optics, enabling sensitive and flexible, top or bottom reading fluorescence measurements. Filters provide sensitivity and access to advanced detection technologies – such as TR-FRET, HTRF® and FP – whereas monochromators offer you free wavelength selection between 280 and 850 nm, plus spectral scanning (excitation and emission) for fluorescent dyes. No matter the optical choice, you still get great performance in a proven instrument, helping to drive your research forward. For a proven fluorescence top and bottom performance the Infinite 200 PRO supports all black microplates of the Tecan microplate portfolio. Show more Filter-based fluorescence optics The Infinite F200 PRO’s filter-based fluorescence optics represent an affordable solution for both basic assays and advanced screening applications, such as FP, HTRF and TR-FRET. The reader’s filter slides incorporate an ID chip that allows filter definitions to be saved directly on the slides. This makes filter slide handling very convenient, as the system will automatically recognize the filters installed on each slide. Standard applications, such as fluorescence-based DNA/RNA quantification assays, can be performed in both microplates and Tecan’s low volume NanoQuant Plate. A dedicated bottom reading optical fiber offers improved performance and additional functionality for cell-based assays. Show more Dichroic mirror The filter optics for top reading fluorescence measurements include a dichroic mirror which dramatically improves the limit of detection (LOD) for dyes that are excited below and emit light above 510 nm. This ensures a typical LOD of just 85 amol/well (384-well plate, 100 µl) for fluorescein-based measurements, allowing you to perform advanced screening assays. HTRF certified The Infinite F200 PRO’s top reading fluorescence optics are certified for HTRF, and offer especially good performance in white, low volume 384-well plates. This set-up offers very good value compared to high-end HTRF readers, giving you 384-well plate throughput at a fraction of the cost. Monochromator-based fluorescence optics The ability to choose any wavelength at any time allows you to run almost any fluorescence-based assay with just a couple of clicks – that is the true advantage of the system’s Quad4 Monochromators. This set-up also gives you the unique ability to characterize the fluorescent properties of dyes and compounds by conducting spectral scans. Pairing these features with a dedicated bottom reading optical fiber for cell-based assays gives you greater flexibility for virtually any fluorescence application. Zoom Automated z-focusing Automatic adjustment of z-focusing for top reading fluorescence measurements makes it easy to miniaturize your assays on the Infinite M200 PRO. This ensures high sensitivity for all plate formats, offering you near-identical performance in up to 384-well low volume plates. This feature is complemented by integrated background correction, providing automatic optimization of the signal-to-noise ratio for every assay. Zoom Fluorescence spectral scanning Quad4 Monochromators technology enables highly sensitive scanning of fluorescent signals. Both excitation and emissions scans can be easily set up in the system’s i-control software, allowing rapid assay optimization. This ensures that you can always produce the best possible signal acquisition for your fluorescence assays. Zoom Key applications DNA/RNA quantification (eg. PicoGreen®, RiboGreen®) Protein quantification (eg. NanoOrange®) ELISAs ORAC assays Cell-based assays (viability, cytotoxicity, etc.) Fluorescence polarization (eg. PolarScreen®) TRF (eg. DELFIA®) FRET (eg. GeneBLAzer®) TR-FRET (eg. HTRF®, LanthaScreen®, Transcreener®) Dye and compound characterization (excitation- and emission scans) Literature Fluorescence-Based DNA Quantification in Small Volume Samples This note describes the implementation of the Infinite 200 PRO multimode reader and the associated NanoQuant Plate for fluorescence-based DNA quantification in small volume samples using Pico Green®, an ultra-sensitive fluorescent dye for the quantitation of double-stranded DNA. ORAC Assay for the determination of antioxidant capacity in foods This application note describes the use of the Infinite® F200 PRO in combination with a commercially available ORAC assay kit, using different beverages as antioxidant samples. Implementation of HTRF® Assay Technology on Infinite® F200 PRO The present application note summarizes the results of experiments with the HTRF (Homogeneous Time-Resolved Fluorescence, Cisbio Bioassays) technology on Infinite F200 PRO. Transcreener® ADP2 Fluorescence Intensity Assay This technical note describes the successful implementation of the Transcreener Fluorescence Intensity Assay on Tecan’s quad-4 monochromator-based Infinite M200 PRO and Tecan’s filter-based Infinite F200 PRO multimode reader. Transcreener® ADP2 TR-FRET Red Assay The Transcreener ADP2 TR-FRET Red Assay is intended for the detection of ADP production by any kinase or ATPase, and has been developed specifically for high throughput screening applications. The assay is based on the competitive binding of ADP to a monoclonal antibody-terbium conjugate, and uses a homogeneous TR-FRET detection mechanism. LanthaScreen® TR-FRET Assay- Implementation on Tecan´s Infinite® F200 Multimode Reader This Technical Note describes the successful implementation of Invitrogen’s LanthaScreen TR-FRET assay system on Tecan’s Infinite F200 PRO filter-based multimodular detection system. The Infinite F200 has performed according to Invitrogen’s LanthaScreen certification program criteria and was successfully validated by Invitrogen as “LanthaScreen® Certified”. DNA and RNA quantification: fast and simple with PicoGreen® dsDNA and RiboGreen® RNA quantification reagents This note describes the use of the fluorescence microplate reader Tecan Infinite F200 PRO or M200 PRO, for detection of as lias 20 pg dsDNA in a 200 μl assay volume. Tweaking fluorescence scans This Technical Note describes the relevant instrument parameters to acquire optimal fluorescence excitation and emission spectra on the Infinite M200 PRO. Cell Proliferation and Cell Viability Analysis in in vitro Systems- Cell Culture Methods on Tecan´s Infinite® 200 In this Application Note the three assay systems introduced above (i.e. MTT assay, CellTiter96 AQueous One Solution Cell Proliferation assay (MTS) and CellTiter-Blue Cell Viability Assay (resazurin) are compared using the human cholangiocarcinoma cell line CCLP-1. The compatibility of these assay systems with Tecan´s Infinite M200 PRO Quad4 Monochromators-based multimode reader is demonstrated. Luminescence Multiple applications, dynamic results The Infinite 200 PRO is adept at producing high quality luminescence measurements for glow, flash and dual-color applications. It features an optical fiber optimized for up to 384-well plates, plus optional 384-well injection capabilities, delivering great sensitivity and a large dynamic signal range for your assays. Engineered to deliver an industry-leading dynamic range and to allow you to use higher density plate formats, this sophisticated luminescence module can replace a standalone luminometer, saving you time and money. For a proven luminescence performance the Infinite 200 PRO supports all white microplates of the Tecan microplate portfolio. Show more Zoom Glow luminescence The luminescence optics in Tecan readers are based on a unique concept; an optical density filter in the module’s filter-wheel attenuates strong signals by a factor of up to 100, but only if you really need it. Combined with a dedicated photomultiplier tube (PMT) set up for single photon counting, this eliminates the conflict between sensitivity and dynamic range commonly associated with instruments that use a single PMT for fluorescence and luminescence measurements. This unique optical combination gives you superior sensitivity for glow luminescence measurements (typically ~225 amol/well ATP), and an industry-leading dynamic range of 108. For most glow luminescence-based assays (such as ATP quantification, chemiluminescent ELISAs, reporter assays, etc.) it is beneficial to miniaturize the assay, as the signal strength is not directly related to the sample. Moving to higher density plate formats therefore saves you time and money. The Infinite 200 PRO uses a luminescence fiber optimized for 384-well plates, guaranteeing minimum signal cross-talk between wells, a critical factor when working in higher density plate formats. Show more Zoom Flash luminescence The Te-Inject is the optional injector module for the Infinite 200 PRO, and offers automatic 384-channel injections with both injectors. This enables highly sensitive ATP quantification assays, such as the ENLITEN® ATP assays, as well as high throughput cell-based reporter assays. The optical design of the luminescence module guides light through a filter wheel equipped with both color and attenuation filters, which is then detected by a dedicated PMT. The attenuation filter allows the PMT to operate in its most sensitive mode while still providing maximum performance for both weak and strong signals. Combined with the Te-Inject, this ensures a very low LOD (typically 12 amol/well ATP) in a 384-well plate (ENLITEN ATP assays, Promega). Thanks to its dual-channel injector module, the Infinite 200 PRO is one of the few DLReady™ readers on the market, meaning it is certified for Promega’s Dual Luciferase Reporter® assays. This technology requires the injection of two different solutions into the same well of a 384-well plate – a technical requirement not supported by most readers. Show more Zoom Dual Color luminescence The Infinite 200 PRO’s luminescence filter wheel is equipped with four color filters, allowing you to perform sensitive dual-color luminescence applications such as NanoBRET™, BRET2™, ChromaGlo™ and others. The Infinite 200 PRO is also equipped with dedicated NanoBRET filters, tested and validated at Promega. This technology allows you to study protein-protein interactions using NanoLuc® Luciferase as the BRET donor and HaloTag® protein (labeled with the NanoBRET 618 fluorophore) as the acceptor. This assay format is designed to offer improved spectral overlap, increased signal strength and lower background signals compared to conventional BRET assays, providing you with a reproducible method for monitoring and screening protein interactions in live cells. Show more Key applications ATP quantification Luminescence reporter assays BRET2 NanoBRET ChromaGlo Dual Luciferase Reporter gene assays Chemiluminescent ELISAs Ca2+ release assays Literature Infinite® 200 PRO – luminescence sensitivity This Technical Note describes optimized test set-ups, instrument settings and the statistical equations that were used to determine the maximum instrument sensitivity of the Infinite F200 PRO filter-based microplate reader and the Infinite M200 PRO Quad4 Monochromators-based microplate reader for flash and glow luminescence. Determination of microbial cell viability The BacTiter-Glo Microbial Cell Viability Assay was tested on the Infinite F200 PRO filter-based multimode reader, using the instrument’s highly sensitive luminescence module. Dual-Luciferase® Reporter Gene Assay This note describes the Dual Luciferase Reporter Assay performance using Tecan’s Infinite 200 PRO multimode reader equipped with a two channel injector. Implementation of NanoBRET Assay™ Technology Dynamic interactions between proteins are key mediators of multiple cellular functions and are involved in almost all biological processes. Accordingly, they are valuable targets for the development of novel drug therapies. In this context, bioluminescence resonance energy transfer (BRET), developed by Promega, is a well-established detection technology for the investigation of protein-protein interactions in living cells. However, its application has so far been somewhat hampered by its limited dynamic range and sensitivity. Absorbance Filter Monochromator 6 - to 384-well plates 6 - to 384-well plates Filter optics Quad4 Monochromators™ optics 230-1,000 nm; filter dependent, 4 positions 230-1,000 nm; 1 nm resolution Low volume NanoQuant Plate™ Low volume NanoQuant Plate™ Cuvette port Fluorescence Filter Monochromator 6- to 384-well plates 6- to 384-well plates Top and bottom reading Top and bottom reading Filter optics Quad4 Monochromators optics 10 W, 40 Hz xenon flash lamp 10 W, 40 Hz xenon flash lamp 50 % dichroic mirror Fixed bandwidth: Ex = 9 nm, Em = 20 nm TRF, FRET, TR-FRET and FP Spectral scanning in top and bottom reading modes, TRF, FRET Luminescence 6- to 384-well plates 384-channel optical fiber Glow luminescence Flash luminescence OD 2 attenuation filters Dual-color luminescence, incl. BRET Temperature RT + 5°C to 42°C Te-Inject™ - reagent injectors 6- to 384-well plates Light protection cover Single or dual dispenser Accessories MultiCheck™ plate for QC and IQOQ Connect™ microplate stacker External barcode scanner NanoQuant Plate for low volume samples i-control™ Software designed for your workflow The Infinite 200 PRO plate reader family is supplied with the easy-to-use i-control software interface, allowing you quickly define a workflow for each of your applications. Intuitive, workflow oriented software. Complex workflows made easy i-control allows you to create a workflow for each application, using ‘drag and drop’ processing steps to generate your assay protocol, which can be saved for future use. Dedicated applications with built-in data reduction The software includes special applications for quick and easy DNA/RNA quantification and labeling efficiency determination, reducing the need for user input. Highlights Real-time data export Direct export to Excel for convenient data reduction. All your data can be automatically exported to Windows®-compatible formats, such as Excel, making data reduction easy and convenient. The instrument automatically records all well-specific data, reader settings and additional information, such as time stamps or temperatures, ensuring you have a complete picture. Show more Extended dynamic range Detection of very low signals. Automatic adjustment of gain settings during a measurement means you never have to compromise on sensitivity, allowing the detection of very low signals. i-control takes care of everything, correlating and displaying all of your results in one single data set. Show more Automated z-focusing Increased sensitivity for fluorescence. Significantly improve the quality of your results with enhanced sensitivity in top reading fluorescence intensity mode. Automatic z-focus adjustment determines the focal point for the best signal-to-blank ratio in each microplate well, regardless of the plate type, well shape or sample volume. This unique feature makes it easy to set up your reader for optimum performance with varying assay parameters. Show more Magellan™ Let Tecan care about your routine data calculations Get more from your microplate reader with Magellan, the universal reader control and data analysis software from Tecan. Magellan supports the complete range of Tecan microplate readers, enabling quick and easy measurement of samples for first-time and expert users alike. A versatile user interface and powerful data reduction capabilities enable the optimization of sophisticated experimental work, making routine tasks easy. Read more Add-ons and QC-tools for Infinite 200 PRO Te-Inject™ - reagent injectors Optional reagent injectors give you valuable dispensing capabilities for any assay, unlocking new applications and workflows in your lab. Injectors are critical for many assays, most notably flash luminescence and calcium flux assays (Fura-2, Fluo-4, etc.). The ability to inject and immediately start measuring is crucial for collecting accurate data. The Infinite 200 PRO’s Te-Inject module comes with a 1 ml syringe, and can be equipped with one or two channels. The software allows you to easily select an injection volume in 1 µl increments, with a dead volume of just 100 µl. Show more NanoQuant Plate™ This device allows parallel quantification and analysis of up to 16 nucleic acid or protein samples, in sample volumes as little as 2 µl. For convenience and optimal data quality, the NanoQuant Plate is the only low volume plate on the market that is 100 % calibration free. This saves time and gives you consistent and reliable performance. In addition to standard absorbance measurements, the NanoQuant Plate is compatible with top reading fluorescence measurements (eg. for PicoGreen® or RiboGreen® assays), improving your DNA/RNA detection limits. Show more Microplates Tecan Microplates for absorbance, fluorescence and luminescence measurements as well as cell imaging in SBS format. Tecan readers and washers are designed to be compatible with ANSI/SLAS (SBS) format. Black and white biochemical assay plates designed for fluorescence and luminescence measurement assays. Low auto phosphorescence of white plates for good quality data and reduced pre-measurement incubation, and a unique combination of black plates with black lids for using SPARK lid lifting system for uninterrupted assays. A collection of tissue-culture (TC) treated, sterile cell culture plates with individual lids for confluence and bottom-fluorescence measurements of cell-based applications. Performance assured with minimum background signal. Show more Connect™ – microplate stacker The Connect is a versatile microplate stacker designed to allow walkaway batch processing of up to 50 plates per run. Read more MultiCheck™ – QC package Gain a new level of confidence in your laboratory equipment with this accurate, cost-effective and near-effortless solution. The MultiCheck QC package is designed to enable rapid functional checks for Tecan multimode readers, and supports all major reading modes, including FI, TRF, FP, absorbance and luminescence. It consists of the MultiCheck software package and an advanced QC plate, with IQOQ documentation also available for the Infinite 200 PRO. Read more Cuvette port For higher sample volumes, an integrated cuvette port in the Infinite 200 PRO allows highly accurate and precise absorbance measurements of samples, eliminating the need for a stand alone cuvette spectrophotometer. List of scientific citations Brochure The Infinite 200 PRO is an easy-to-use multimode plate reader family that offers affordable, high performance detection solutions, referenced in more than 1800 peer reviewed publications. Read the infinite brochure Typical performance values Infinite 200 PRO instrument specifications and typical performance values... See the typical performance values Related products Spark® The latest Spark multimode microplate reader – your daily research partner. Microplate washers Designed to provide optimized washing of cells, beads and ELISAs. Sunrise™ A versatile microplate absorbance reader for 96-well plates. Infinite® F50 Innovatively designed, next generation, 8-channel absorbance plate readers. 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