--> PerkinElmer - Cisbio Home Life sciences Kinome investigation Exploring the high-potential world of kinases Your focus is kinase research? Cisbio has the solutions to help you move forward. The human kinome comprises 518 protein kinases and 20 lipid kinases. These proteins are involved in a wide variety of cellular processes, including cycle mechanisms, metabolism, transcription, cytoskeletal development, cellular motility, apoptosis, cell proliferation, differentiation, and even intracellular communication (Manning, G. et al. 2002. The Protein Kinase Complement of the Human Genome. Science (298):1912-1934). Kinase white paper It’s time for you to find out more about the kinome world and its implication in diseases! Read this highly informative white paper written by Cisbio in collaboration with Labiotech.eu. Read the white paper Kinase research made easy using HTRF® technology Working tirelessly to match your needs, Cisbio experts designed and developed multiple solutions for studying kinases, from kinase activity to ATP-competitive inhibitor binding. femme_ipad Kinase activity Next Kinase activity Universal solution or target-specific substrates? Cell-based or biochemical assays?The choice is yours! A universal solution: KinEASE assays Cisbio experts developed specific HTRF® KinEASE kits to investigate kinase activity, characterize kinases, and screen for inhibitors. The five kits in the product range offer a semi-universal method for addressing phosphorylation on Serine/Threonine and Tyrosine residues, respectively. Over 272 kinases have already been validated with this method. The principle for these assays is divided into two main steps: Enzymatic step: during the enzymatic step, the tagged substrate is incubated with the kinase of interest and ATP, allowing the phosphorylation reaction to begin. Detection step: detection reagents are added and recognize the phosphorylated substrate. The resulting Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) signal is proportional to the phosphorylation level. Measuring kinase activity under nearly any condition Based on HTRF® technology, the KinEASE kit is used as a universal tool to characterize Serine/Threonine and Tyrosine kinase activity. Read this document and find out more about the method. Download now Addressing multiple therapeutic areas with HTRF® KinEASE assays Check out this literature to discover three case studies using KinEASE assays in various therapeutic areas (cancer, neurological and autoimmune diseases). Download now A complete overview of the KinEASE assay Understand how the KinEASE assay works and watch a clear demonstration using HTRF® KinEASE – STK S1. A substrate-specific solution: LANCE® Ultra Kinase Assay LANCE® technology (LANthanide Chelate Excitation) Ultra is a solution in which your fluorescently labeled substrate can be chosen specifically. Over 300 kinases were tested and validated with this approach. The assay protocol is divided in two steps: Enzymatic step: the ULight-peptide substrate is phosphorylated in the presence of kinase and ATP. Detection step: the substrate is captured by an Eu-anti-phospho-substrate antibody. Upon excitation, the Eu-chelate transfers its energy to the ULightTM dye, resulting in fluorescent light emission at 665 nm. For more information on the technology and its various applications, be sure to have a look at the dedicated web page. Learn more about LANCE® Ultra platform LANCE® Ultra Kinase Guide This document helps you on your substrate choice and the corresponding Europium-labeled anti-phospho antibody. Download now Cell-based solution Cellular protein phosphorylation assays can be used for cell-based assays, providing the sensitivity required to detect endogenous and basal levels of phosphorylated proteins. Read our dedicated “Phosphorylation from A to Z” documentation to learn more about this solution. The Cell Signaling guide includes an overview of the assay. You will also find a complete assay description, case studies, applications and more. Download the guide Kinase Activity Next Enzymatic buffer kinase 5X HTRF KinEASE detection buffer HTRF KinEASE STK Discovery kit HTRF KinEASE-STK S1 kit HTRF KinEASE-STK S2 kit HTRF KinEASE-STK S3 kit HTRF KinEASE-TK kit SEB Supplemented Enzymatic Buffer STK-Substrate 1-biotin STK-Substrate 2-biotin STK-Substrate 3-biotin TK Substrate-biotin See all products PerkinElmer - Cisbio Get on the list to receive remarkable content Subscribe Life sciences In Vitro Diagnostic Cisbio Select your country Follow us This website stores cookies on your computer. These cookies are used to collect information about how you interact with our website and allow us to remember you. We use this information in order to improve and customize your browsing experience and for analytics and metrics about our visitors both on this website and other media. OK, accept all Deny all cookies Personalize Privacy policy