PORTFOLIO COMPANION DIAGNOSTICS ENABLING TECHNOLOGIES RESOURCES OUR CULTURE About Contact Careers Search HomePortfolioGeneticsAmplideX® PCR/CE HTT Kit AmplideX® PCR/CE HTT Kit The AmplideX PCR/CE HTT Kit* makes the detection and sizing of CAG trinucleotide repeats in the HTT gene easier than ever. In just a single PCR reaction, the assay overcomes the challenges of adjacent SNPs and variable CCG repeats to accurately and reliably report the number of repeats in one day, from as little as 10ng gDNA. Features & Benefits Analytical Characteristics Ordering Information Features & Benefits The AmplideX PCR/CE HTT Kit includes reagents to perform a two-primer, anchor-primed PCR of the HTT CAG repeat region followed by fragment sizing by capillary electrophoresis. This single-tube PCR reaction provides a streamlined workflow, enabling sample-to-result in approximately 6 hours using readily available laboratory equipment. Reduced Complexity Proprietary PCR solutions for GC-rich amplification and detection Eliminates need for multiple PCRs – one result, straightforward analysis Resolves zygosity and detects large expansions Optimized Workflow Fully kitted, end-to-end solutions that significantly reduce hands-on-time Sample-to-result possible within a single shift Identical PCR and CE conditions as the AmplideX PCR/CE DMPK Kit Quality Results Reliable, unambiguous results and a robust stutter peak pattern Accurate sizing across the entire CAG repeat range Software converts raw base pair data into number of repeats *For Research Use Only. Not for use in diagnostic procedures. Analytical Characteristics Excellent Concordance with Previously Characterized Samples (Table 1) Repeat profile is conserved across known SNPs (Figure 1) Clearly resolves zygosity (Figure 2) Table 1. Comparison of CAG repeat alleles in commercially available samples, as reported by Kalman et al. (2007) Figure 1. Repeat profile is conserved across known SNPs. Figure 2. Zygosity is clearly resolved using the AmplideX PCR/CE HTT Kit. Figure 3. Workflow for the AmplideX PCR/CE HTT Kit. Ordering Information Product Name Number of Reactions Catalog Number AmplideX® PCR/CE HTT Kit (RUO) 32 49657 T 1-877-777-1874; 512-681-5200 F 512-681-5202 E orders@asuragen.com VIEW SALES CONTACTS Asuragen Genetics AmplideX® Fragile X Dx & Carrier Screen Kit AmplideX® mPCR FMR1 AmplideX® PCR/CE FMR1 AmplideX® FMR1 Controls Xpansion Interpreter® AmplideX® PCR/CE SMN1/2 Plus Kit AmplideX® PCR/CE CFTR Kit AmplideX® PCR/CE C9orf72 Kit AmplideX® PCR/CE DMPK Kit AmplideX® PCR/CE HTT Kit QUICK LINKS Download Product Brochure Change Location US Non-US I acknowledge that the information on this site is for non-US customers only. The site may contain information that is not approved for your country. View Sales Contacts 2150 Woodward St., Suite 100 Austin, TX 78744 USA PORTFOLIO COMPANION DIAGNOSTICS ENABLING TECHNOLOGIES RESOURCES COMMUNITY About Contact Careers © Copyright 2021 ASURAGEN, INC. An Equal Opportunity Employer. All Rights Reserved. Privacy Policy Website Terms of Use Financial Conflict of Interest Policy