50 cm µPACᵀᴹ column The 50 cm µPACᵀᴹ column is the column of choice in a routine proteomics research setting where improvements in reliability are needed aside excellent chromatographic performance. With an internal volume of 3 µL, this column is perfectly suited to perform high-throughput analyses with shorter solvent gradient times (30-, 60- and 90-minute gradients), even for limited sample amounts. The column is compatible with all commercially available nano-LC systems, and can be integrated smoothly in any experimental set-up. Must be used in combination with a grounding cable and preferably with a connecting capillary. Order now This column offers: Increased separation performance More than double the number of peptides and 72% more proteins identified in a 60 min analysis compared to a 15 cm packed bed column. Find out more in this technical note. Improved coverage The ultra-sensitive analysis of low-input proteomics samples enabled twice as many unique peptide and protein groups identifications compared to a traditional column. Discover the results in our webinar. Extreme robustness Each column has been manufactured by etching channels and pillar arrays out of a solid silicon wafer. The column is perfectly bidirectional and contains no particles or frits. This prevents blockages and retention time variation over multiple runs. Run your entire project on a single column. Increased column to column reproducibility Each column is manufactured using the same lithographic mask, making every column identical. Replace µPACᵀᴹs with minimal impact. Obtain results over time and across laboratories, sufficiently consistent for big data analysis. High flow rate flexibility The column can be operated at moderate LC pump pressures up to 350 bar over a wide range of flow rates (100 – 2,000 nL/min). The maximum operating pressure is 350 bar. RELATED MEDIA All Webinar Publications Technical notes Videos Publications New format for PharmaFluidics’ μPAC™ columns to meet surging customer demand Over the recent months, PharmaFluidics has focused on scaling up production of its μPAC™ nano-LC columns in response to a surge in customer demand and … Read More → Webinar Webinar – Tips and tricks when working with PharmaFluidics’ micro Pillar Array Columns Tips and tricks when working with PharmaFluidics’ micro Pillar Array Columns Compared to traditional LC columns, PharmaFluidics uses a fundamentally different approach to fabricate their … Read More → Webinar Webinar – Expanding the limits of routine proteomics using 50 cm micro Pillar Array Column (µPAC™) Expanding the limits of routine proteomics using 50 cm micro Pillar Array Column (µPAC™) The practice of bottom-up proteomics relies to a large extent on … Read More → Webinar Webinar – A New LC Approach to Complex Biological Samples by using micro Pillar Array Columns A New LC Approach to Complex Biological Samples by using micro Pillar Array Columns In the last decades, several advances have been made in liquid … Read More → Webinar Webinar – Practical applications of µPAC™ column technology using data-independent acquisition for clinical proteomics In this webcast, several workflows will be shown. The first part will discuss our collaboration with the researchers from Biognosys, working on a comprehensive proteomics … Read More → Publications Perfecting Proteomics Separations An innovative award-winning micro-chip chromatography ‘column’, µPACᵀᴹ promises high-resolution separations of complex biological samples. Prof Kris Gevaert and Dr Francis Impens, from Ghent University and … Read More → 1 2 3 Next » STAY UP TO DATE ON OUR LATEST INNOVATIONS Subscribe Copyright 2020 PharmaFluidics Technologiepark-Zwijnaarde 82, B-9052 Ghent (Zwijnaarde) T +32 (0)9 241 56 57 Privacy Policy & Disclaimer Cookie settings ACCEPT