Company Resources Products Applications Safety Contact Us Products Life Sciences Biocatalysis Chromatography - Synthetic resins Chromatography - Agarose resins Solid Phase Peptide & Oligonucleotide Synthesis Shallow Shell™ Adsorbents Chromatographic Separation Resins Catalysts Chelation Resins Hydrometallurgy Nuclear Power Powdered Resins for Condensate Polishing Pharmaceutical - API's Potable Water Ultrapure Water Strong Acid Cation Gel Strong Acid Cation Macroporous Weak Acid Cation Strong Base Anion Gel Strong Base Anion Macroporous Weak Base Anion Gel Weak Base Anion Macroporous Ready to Use Mixed Beds Inert/Spacer Resins Chromatography - Agarose resins Products > Life Sciences > Chromatography - Agarose resins > Praesto™ - Agarose resins Ion exchange chromatography (IEX) separates proteins and other biomolecules based on their charge. IEX resins have charged functional groups that bind molecules with an opposite charge. Bound molecules are most commonly eluted by an ascending salt gradient. Alternatively, a shift in pH that changes the charge of the target molecule can be introduced. The most common ion exchangers are based on sulphopropyl chemistry (strong cation exchangers, Praesto SP) or quaternary amine chemistry (strong anion exchangers, Praesto Q) and maintain their charge over a wide pH interval. Praesto SP and Praesto Q are high performance ion exchange resins optimized for large scale purification of recombinant proteins such as monoclonal antibodies and other medium sized biomolecules. Affinity chromatography is a high resolution technique used for the purification of biomolecules. Affinity chromatography resins are produced by coupling ligands that specifically binds to the target biomolecule to a chromatographic matrix. The most common example is Protein A affinity chromatography where Protein A is covalently coupled to a base matrix and subsequently used for purification of monoclonal antibodies. Praesto Pure resins are the ideal base matrix for production of affinity chromatography resins with minimal unspecific interaction and long functional life time. Gel filtration also commonly called size exclusion chromatography (SEC), is used to separate molecules on the basis of their molecular size. Praesto Pure has a very high exclusion limit and can be used to purify large macromolecules, for example viruses and plasmids, from smaller molecules such as host cell proteins or DNA fragments. Available particle sizes. To cover most possible applications the Praesto family of agarose resins are available in three different particle sizes, 45, 65, and 90 μm mean particle diameter. A smaller particle size will result in better efficiency (higher plate number) which translates to higher dynamic binding capacity and better resolution. However, the small particle size comes at the cost of increased column back pressure and thus a lower maximum flow rate. The optimal particle size for a particular application will thus depend on a number of different parameters. Examples are final scale, resolution, productivity, and cost. For further information contact us Product Name Application Ionic Form PraestoTM SP45 Protein purification N/A PraestoTM SP65 Protein purification N/A PraestoTM SP90 Protein purification N/A PraestoTM Q45 Protein purification N/A PraestoTM Q65 Protein purification N/A PraestoTM Q90 Protein purification N/A PraestoTM Pure45 Protein purification N/A PraestoTM Pure65 Protein purification N/A PraestoTM Pure90 Protein purification N/A Quick Links Home Company Resources Products Applications Safety Contact Sitemap Americas T +1 800.343.1500 americas@purolite.com Europe T +44 1443 229334 europe@purolite.com Asia Pacific T +86 571 876 31382 asiapacific@purolite.com © 2013 PUROLITE This site is protected by copyright and trade mark laws under U.S. and International law. All Rights Reserved. Terms & Conditions Privacy Policy Export Compliance Statement