PRODUCTS TECHNOLOGY RESOURCES CONTACT US EVERYTHING YOU NEED RESOURCES Access detailed manuals and instructions on how to perform the protocol. Get guidelines on instrumentation and reagents for performing Spatial Transcriptomics in-house. Tissue Optimization Adapt the ST protocol to your specific fresh frozen tissue type Library Preparation & Sequencing Prepare Spatial RNA-seq libraries from intact tissue sections. Data Processing & Analysis Data is intuitively processed and analysed with tailored bioinformatics tools. Orders Purchase our glass slides and perform Spatial Transcriptomics experiments in-house. GETTING STARTED Where can I find the Tissue Optimization Manual? Are instructional videos available? How should I store TO/LP slides? What tissue types can be used with the Spatial Transcriptomics technology? What quality control measures should I take before starting a Tissue Optimization experiment? How should tissue be frozen? Do I need to perform a Tissue Optimization experiment if I know the optimal permeabilisation and tissue removal conditions for my tissue of interest? How big can my tissue section be? Where can I find the Tissue Optimization Kit (discontinued) Safety Data Sheets? USING IT Can I use a fixative other than formaldehyde to fix my tissue? Do I need to include the positive and the negative control in my Tissue Optimization experiments? How should I image my H&E stained sections? How can I image the fluorescent cDNA footprint? RESULTS What is the expected result of a Tissue Optimization experiment? TROUBLESHOOT Tissue was not entirely removed after tissue removal step. Why can’t I detect a fluorescent signal at the end of my Tissue Optimization experiment? CONTACT 10x Genomics CONTACT SUPPORT support@10xgenomics.com FOLLOW