VIDEO GALLERY TOSOH BIOSCIENCE WORLDWIDE TOSOH GLOBAL English option Click to collapse HOME SEPARATIONS & PURIFICATION CLINICAL DIAGNOSTICS LEGAL The Chemistry of Innovation By bringing the best in quality products, services and technologies from Tosoh Corporation, Tosoh Bioscience is dedicated to pioneering systems used to assist in monitoring life-threatening diseases and certain cancers, helping to prevent epidemics, purify water, monitor our environment, and making living better for everyone, everywhere. Links from this site take you to separate Tosoh Bioscience subsidiary websites. Separations & Purification Portal 500+ specialty products in analytical HPLC columns, chromatographic resins, and GPC instruments to meet your laboratory needs. Find a global Tosoh separations & purification subsidiary near you. More Clinical Diagnostics Portal Superior automated immunoassay and glycohemoglobin analyzers, laboratory solutions, assays, and test-cup reagents. Find a global Tosoh clinical diagnostics subsidiary near you. More Home Tosoh Sitemap

VIDEO GALLERY TOSOH BIOSCIENCE WORLDWIDE TOSOH GLOBAL English option Click to collapse HOME SEPARATIONS & PURIFICATION CLINICAL DIAGNOSTICS LEGAL Video - Ion Exchange Chromatography HOME VIDEO Separations & Purification Ion Exchange Chromatography Separations & Purification Principles of Chromatography Ion Exchange Chromatography Hydrophilic Interaction Chromatography Reversed Phase Chromatography Size Exclusion Chromatography Hydrophobic Interaction Chromatography Mixed Mode Chromatography Affinity Chromatography Clinical Diagnostics VIDEO TEXT: Biomolecules generally have charged groups on their surfaces, which change with the pH of the solution. This is the basis for Ion Exchange Chromatography (IEC), in which molecules reversibly bind to an oppositely charged group of the packing material. Molecules with a higher charge density bind more strongly to the resin. The bound sample may be selectively removed from the stationary phase by changing pH or salt concentrations of the mobile phase. The higher the charge of the molecules and the stronger the binding to the stationary phase, the greater the change in the salt concentration required. IEC is a powerful separation tool because of its high selectivity and capacity. The technique is used for a variety of samples and is particularly effective for proteins. Home Tosoh Sitemap