Products Applications Custom Resources Support About Home Products Sequencing Sanger Sequencing ProDye™ Terminator Sequencing System Share Sanger Sequencing Chemistry for Capillary Electrophoresis Platforms Easily implemented with the same protocol, workflows and calibration Designed for a wide range of sequencing applications Provides enhanced productivity while reducing costs Size 24 reactions 200 reactions 1000 reactions Catalog number selected: CR4324 € 278,00 Your price: Log in Add to Cart Add to Helix Overview Protocols Specifications Resources Related Products Sanger Sequencing in Perfect Harmony with Your Workflow The ProDye™ Terminator Sequencing System provides an improved method of fluorescent Sanger sequencing using a proprietary thermostable DNA polymerase included in the master mix. Compatible with a variety of DNA templates, the ProDye™ Terminator Sequencing System can be used with the Spectrum Compact CE System as well as the Applied Biosystems® 3130, 3130xl, 3500, 3500xL, 3730, 3730xl and SeqStudio® Genetic Analyzers. The ProDye™ Terminator Sequencing System uses dTMR, dCXR, dRSixG and dROneTen terminator dyes, the same dyes contained in the BigDye® Terminator V3.1 Cycle Sequencing Kit. Your Sample, Our Sequencing Kit, Any Instrument Integrate the ProDye™ Terminator Sequencing System seamlessly into a familiar workflow. Familiar Reaction Setup Includes all the components for the sequencing reaction in a ready-to-use, premixed format—just add your template and the template-specific primer. Proprietary enzyme blend in 2.5X Master Mix Additional 5X Sequencing Buffer and Nuclease-Free Water is included Supports 10μl reactions and 20μl 0.5X reactions Compatible with a Variety of Thermal Cyclers Simple cycling protocol designed for obtaining maximum read lengths. Compatible with a Variety of Samples and Running Conditions Sequence plasmid DNA and other samples using 'Standard' or 'Fast' sequencing protocols. Chromatograms from NIST plasmid ERCC-00083 cycle sequenced with ProDye™ Terminator Sequencing System and detected on the Spectrum Compact CE System using the ‘Standard’ sequencing protocol. Plasmid ERCC00083 from NIST was cycle sequenced with ProDye™ Terminator Sequencing System. Sequencing reactions were purified with ethanol/EDTA-based precipitation and denatured in Hi-Di™ Formamide before detection on the Spectrum Compact CE System using the T_Seq_36_Std protocol (Panel A) and T_Seq_36_Fast protocol (Panel B). Chromatograms from control pGEM®-3Zf(+) plasmid cycle sequenced with ProDye™ Terminator Sequencing System and detected on the Spectrum Compact CE System using the ‘Standard’ sequencing protocol. pGEM®-3Zf(+) plasmid DNA was cycle sequenced with ProDye™ Terminator Sequencing System. Sequencing reactions were purified with ethanol/EDTA-based precipitation and denatured in Hi-Di™ Formamide before detection on the Spectrum Compact CE System using the T_Seq_36_Std protocol (Panel A) and T_Seq_36_Fast protocol (Panel B). Sequencing Analysis Read Lengths Combine ProDye™ Terminator Sequencing System with the Spectrum Compact CE System for more bases in less time. Chromatograms from control pGEM®-3Zf(+) plasmid cycle sequenced with ProDye™ Terminator Sequencing System and detected on the Spectrum Compact CE System using T_Seq_36_Std protocol (Panel A) and T_Seq_36_Fast protocol (Panel B). Product Application: Sanger Sequencing of SARS-CoV-2 Sequence a region of the SARS-CoV-2 genome using the ProDye™ Terminator Sequencing System with analysis by capillary electrophoresis on the Spectrum Compact CE System. Download Product Application Learn about the Spectrum Compact CE System ProView™ Sequencing Software ProView™ Sequencing Software is a customizable Sanger sequencing viewer capable of displaying .ab1 files generated on many currently available capillary electrophoresis (CE) sequencers. This software can be used to view and edit sequence information, display electropherograms, generate reports, display embedded information and save edited files in multiple formats. Get ProView Sequencing Software for Free Comprehensive Technical Support We understand that your work can’t stop and we are dedicated to minimizing interruptions to your lab workflow. Whether you are working with a new sample type, implementing Sanger sequencing on a new instrument, or anything in between, you can be confident that your sequencing will hit all the right notes with our comprehensive technical support. Promega scientists are ready to help, and we will be there when you need us. Explore Support Options Protocols Complete Protocol ProDye™ Terminator Sequencing System Technical Manual PDF (5263 KB) – English Specifications You are viewing: CR4324 Change Configuration What's in the box? Item Part # Size ProDye™ 2.5X Master Mix CR100A 1 × 200μl ProDye™ 5X Sequencing Buffer CR101A 1 × 1ml pGEM®-3Zf(+) Vector P227A 1 × 20μg pUC/M13 Primer, Forward (24mer) Q560A 1 × 2μg Nuclease-Free Water P119A-C 1 × 1.25ml SDS Search for SDS Certificate of Analysis Search by lot number Use Restrictions For Research Use Only. Not for Use in Diagnostic Procedures. Storage Conditions Resources Other Resources What Is Sanger Sequencing? What Is Capillary Electrophoresis? Similar Products ProDye™ 4C Matrix Standard DNA fragments labeled with four different fluorescent dyes in one tube for use with the ProDye™ Terminator Sequencing System. CR4500 ProDye™ 5X Sequencing Buffer Optimized for use with the ProDye™ Terminator Sequencing System. CR1011 ProDye™ Sanger Sequencing Standard DNA of a known sequence template for control reactions prepared with ProDye™ Terminator Sequencing System. CR4604, CR4696 Frequently Used With Spectrum Compact CE System An affordable benchtop instrument for Sanger sequencing and fragment analysis. CE1304, CE1307, CE1305 Spectrum Compact CE System Consumables Plug-and-play prefilled consumables with guided software user interface for the Spectrum Compact CE System. CE2300, CE2301, CE2302, CE2304, CE2307, CE2308, CE2332, CE2340, CE2399 Let's find the product that meets your needs. Talk to a Scientist Stephen UK Don't miss out! Stay notified of Promega events, products and news. Sign Up Need Assistance? 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