200 cm µPACᵀᴹ column The 200 cm µPACᵀᴹ column provides the ultra-high resolution needed to extract the maximum amount of information from highly complex samples. Dig deeper into the proteome, metabolome or lipidome, or detect minor contaminations or very subtle variations between biomolecules. This is possible by using longer gradients enabled by the benefits of perfect order. The column is compatible with all commercially available nano-LC systems, and can be integrated smoothly in any experimental set-up. Must be used in combination with a grounding cable and preferably with a connecting capillary. Order now This column offers: Optimal separation performance Obtain more than double the protein identifications than on a 40 cm packed bed column as demonstrated in this webinar. Improved coverage µPACᵀᴹ provides comprehensive coverage in single shot proteome analysis, nearing the mark of 10,000 identifications. Discover the results in our executive summary or webinar. Extreme robustness Each column has been manufactured by etching channels and pillar arrays out of a solid silicon wafer. The column is perfectly bidirectional and contains no particles or frits. This prevents blockages and retention time variation over multiple runs. Run your entire project on a single column. Increased column to column reproducibility Each column is manufactured using the same lithographic mask, making every column identical. Replace μPACᵀᴹs without retention time shifts. Obtain results over time and across laboratories, sufficiently consistent for big data analysis. High flow rate flexibility The column can be operated at moderate LC pump pressures up to 350 bar over a wide range of flow rates (100 – 1,000 nL/min). RELATED APPLICATION NOTES All 200cm 50cm 50cm GEN2 Biopharmaceuticals capLC Host cell proteins Limited sample Lipidomics Metabolomics Proteomics 200 cm Oligonucleotide characterization using μPAC™ nanoLC C18 columns in Ion-pairing reversed phase liquid chromatography (IPRP-LC) mode In this application note, the resolving power of micro Pillar Array Columns (μPAC™) is demonstrated and compared to packed bed columns. Excellent peak capacity and … Read More → 50cm µPAC™ Generation 2: setting a new standard in nanoLC column performance The performance of the 50 cm µPAC™ GEN2 Nano column is demonstrated on LCMS instrumentations of different sensitivity levels. The column is also assessed against … Read More → Flex iON Connect Next generation non-porous µPAC™ columns for ultra-sensitive nanoLC-MS proteome analysis in limited sample workflows The performance of the µPAC™ GEN2 Limited Sample Amount column is assessed against classic packed-bed columns for the bottom-up proteomics when working with low amount … Read More → 50cm Detailed glycosylation analysis of therapeutic enzymes using µPAC™ capLC-MS and all-ion fragmentation The use of micro Pillar Array Columns (μPAC™) for characterizing glycosylation of therapeutic enzymes is presented. Recombinant human acid α-glucosidase (hGAA) was digested and resulting … Read More → capLC High-throughput, routine and comprehensive proteome analysis using a micro Pillar Array Column (μPAC™) based capillary-flow LC-MS workflow In this application note, optimal LC methods for a variety of LC-MS based proteome analysis needs are described. PharmaFluidics’ μPAC™ capLC column is evaluated for … Read More → capLC Bottom-Up Proteomic Profiling at Capillary LC Flow Rates In this application note, the performance of PharmaFluidics’ μPAC™ capLC columns is evaluated in a typical bottom-up proteomics workflow. 500 ng of tryptic digest sample … Read More → 1 2 3 Next » RELATED MEDIA All Webinar Publications Technical notes Videos Technical notes Technical note 1: perfect order promotes permeability Perfect order and its role in highly efficient liquid chromatography on micro pillar array columns (µPACTM) – Part 1: Permeability Introduction As stated by Giddings … Read More → Technical notes Technical note 2: µPACTM performance in gradient separations Perfect order and its role in highly efficient liquid chromatography on micro pillar array columns (µPACTM) – Part 2: Performance in gradient separations Introduction As … Read More → Publications μPAC™ column technology for single cell proteomics Read or download the pdf version of this article here. Next to the development of dedicated low-input sample preparation methods, which aim at reducing sample … Read More → Publications Evaluation of Micro-Pillar Array Columns (µPACᵀᴹ) Combined with High Resolution Mass Spectrometry for Lipidomics Evaluation of Micro-Pillar Array Columns (µPACᵀᴹ) Combined with High Resolution Mass Spectrometry for Lipidomics – Koen Sandra et al – LCGC Europe HPLC 2017 Special … Read More → Publications PharmaFluidics and ThermoFisher present new proteomics results at HUPO2017 In collaboration with ThermoFisher, PharmaFluidics has presented new results on the benefits of µPAC™ in the analysis of proteomic samples. The poster, presented at HUPO2017, specifically … Read More → Publications PharmaFluidics and RIC present data on the comparison of biologicals and candidate biosimilars at BioProcess International 2017 At BioProcess International 2017 in Boston (USA), the results of a collaboration between the Research Institute for Chromatography and PharmaFluidics were presented. This poster gives … Read More → 1 2 3 … 5 Next » STAY UP TO DATE ON OUR LATEST INNOVATIONS Subscribe Copyright 2020 PharmaFluidics Technologiepark-Zwijnaarde 82, B-9052 Ghent (Zwijnaarde) T +32 (0)9 241 56 57 Privacy Policy & Disclaimer Cookie settings ACCEPT