SEARCH MENU × Close By continuing to browse the site, you are agreeing to our use of cookies. Find out more LEARNING CENTER MERCODIA ELISA TECHNOLOGY ATHEROSCLEROSIS DIABETES MELLITUS OBESITY MERCODIA WHITE PAPERS MERCODIA ELISA TECHNOLOGY Mercodia kits are based strictly on stable, monoclonal antibodies combined with other top quality reagents. This ensures safe long-term production with minimal lot-to-lot variation. By the careful selection of reagents, Mercodia products provide accurate results by first run. The links underneath will lead you through the principle of the ELISA technology, the advantages of monoclonal antibodies and the accuracy of Mercodia´s assays. You will learn more about the hook effect and detection limit and how to calculate your results. And last but not least how you can establish your own controls. PRINCIPLE OF TECHNOLOGY ELISA (ENZYME-LINKED IMMUNOSORBENT ASSAY) is a widely used biochemical technique for the detection of an antigen in a sample. READ MORE POLYCLONAL VS MONOCLONAL ANTIBODIES Since the discovery by César Milstein and George Köhler in 1975 on the construction of a continuously growing cell line expressing a specific, predefined monoclonal antibody, it has been possible to produce unlimited amounts of pure and specific monoclonal antibodies. READ MORE WHEN ACCURACY MATTERS When analyzing samples in the ELISA platform, there are a variety of components that are affecting the test results. READ MORE ASSAY SENSITIVITY Sensitivity is a statistical term used to describe the ability of an assay to separate the blank from the specific analyte. Since there are a variety of methods used for determining sensitivity, the method must be known when comparing sensitivity between assays. READ MORE HIGH DOSE HOOK EFFECT The high dose hook effect refers to measured levels of antigen displaying a significantly lower absorbance than the actual level present in a sample. READ MORE CALCULATION OF RESULTS There are several types of curve-fitting methods for computerized calculation. READ MORE ESTABLISHMENT OF CONTROL RANGES AND TARGET VALUES The routine analysis of controls with known concentrations is a valuable tool for the acceptance of test runs and to validate that analyzed sample concentrations are in fact repeatable and reproducible at your laboratory. READ MORE ATHEROSCLEROSIS AND CARDIOVASCULAR COMPLICATIONS CARDIOVASCULAR DISEASE Cardiovascular disease (CVD) is the major cause of death globally. According to WHO an estimated 17.1 million people died from CVD in 2004. READ MORE RISK PREDICTION A developing atherosclerosis is an underlying cause of CVD and it is well accepted that inflammation is implicated in the pathogenesis. READ MORE MEASUREMENT OF OXIDIZED LDL Given the suggested patho-physiological significance of oxLDL, there has been a significant increase in the research of the pathogenic role of circulating oxLDL, giving new insights and questions in regards to the role of oxLDL in cardiovascular disease. READ MORE OXIDIZED LDL IN RISK PREDICTION Is oxLDL, as measured with the Mercodia Oxidized LDL ELISA, just another assay for LDL-cholesterol or does oxLDL provide any additional information? READ MORE REFERENCES References READ MORE DIABETES MELLITUS Diabetes Mellitus is a combination of several different diseases with various etiologies. Diabetes arises when the beta cells of the Langerhans islets in the pancreas is unable to meet insulin demands in the organism, either due to functional failure and destruction of the beta cell mass or because of insulin needs due to insulin resistance or growth that are disproportionate to insulin secretory capacity. This condition has been known for over 3000 years and today diabetes is one of the major health challenges of the 21st century. This non-communicable disease has reached epidemic proportions in all regions of the world and the prevalence of diabetes is expected to continue to rise inexorably if nothing is done to slow this epidemic. The International Diabetes Federation has predicted that the sufferers from diabetes will exceed 350 million by 2025. Estimation of people suffering from diabetes. Zimmer et al., 2001 The two most common forms of diabetes are Insulin Dependent Diabetes Mellitus (IDDM), also called Type 1 Diabetes, and Non Insulin Dependent Diabetes Mellitus (NIDDM), also called Type 2 Diabetes. 5 REASONS SCIENTISTS CHOOSE MERCODIA GLUCAGON ELISAS Learn more about our Glucagon advantages READ MORE MERCODIA GLUCAGON WHITE PAPERS Read Mercodia Glucagon White Papers and learn about the challenges of glucagon measurements READ MORE TYPE 1 DIABETES Type 1 diabetes represents approximately 10% of all cases of diabetes and is often referred to as juvenile-onset diabetes. READ MORE TYPE 2 DIABETES Type 2 diabetes is estimated to represent 90% of all cases of diabetes. READ MORE COMPLICATIONS Today, there is no cure for diabetes; although transplantation of donator islets or whole pancreas yields a very god outcome for a few individuals. READ MORE THERAPY Today, great efforts are made to create centers of education regarding diabetes with purpose to educate the patient in understanding the disease and to get tools for self-help. READ MORE SEQUENCE HOMOLOGY Preproinsulin is translated in the beta-cell cytoplasm after which it is cleaved into proinsulin in the ER and then transported to the Golgi apparatus. READ MORE DOG DIABETES Dogs are susceptible to several diseases that also occur in humans, including diabetes, dental and heart disease, epilepsy, cancer, hypothyroidism, and arthritis. READ MORE DAIRY COWS Dairy cows are bred for the ability to produce large yields of milk, from which dairy products are made. READ MORE CAT DIABETES DIABETES MELLITUS Diabetes mellitus is one of the most frequently diagnosed endocrinopathies in cats. READ MORE SHEEP DIABETES A decrease in dry matter intake is a major physiological change in ruminants. READ MORE EQUINE METABOLIC SYNDROME The term “equine metabolic syndrome” describes a collection of clinical signs that contribute to the development of founder (laminitis) in horses. READ MORE PORCINE MODELS Porcine models have been used in research for decades due to their anatomical and physiological similarities to humans. READ MORE OBESITY OBESITY-AT-A-GLANCE Obesity is a worldwide epidemic, raising serious concerns about health status and health care in the coming years. READ MORE LEPTIN The Study of Obesity: What to Measure READ MORE ADIPONECTIN The Study of Obesity: What to Measure READ MORE ASSOCIATED CONDITIONS AND RELEVANT BIOMARKERS Unfortunately, the negative health consequences of obesity are numerous and range from tolerable to life-threatening conditions. READ MORE CHILDHOOD OBESITY: A CONCERNING EPIDEMIC Obesity is a major risk factor for many chronic diseases, such as diabetes, cancer and cardiovascular disease. READ MORE MERCODIA WHITE PAPERS GLUCAGON Read Mercodia white papers regarding Glucagon as a biomarker READ MORE MERCODIA AB HEADQUARTERS IN UPPSALA, SWEDEN: SYLVENIUSGATAN 8A SE-754 50 UPPSALA, SWEDEN © COPYRIGHT 2017 EMAIL US PHONE + 46 18 57 00 70 FAX + 46 18 57 00 80 HOME PRODUCTS SERVICES LEARNING CENTER WEBINARS AND PODCASTS NEWS & EVENTS VIDEOS DIRECTIONS FOR USE MERCODIA CONTACT HELP & SUPPORT TERMS OF SERVICE INFORMATION ABOUT COOKIES SITEMAP REGISTER LOGIN Visit us on social media: facebook.com/mercodia twitter.com/Mercodia linkedin.com/company/mercodia-ab plus.google.com/113076055858025990989 Share current page