Products Applications Custom Resources Support About Home Products Protein Detection We offer a broad range of tools for detecting and quantifying proteins, from traditional immunological detection reagents to novel methods that monitor protein abundance in live cells. Protein labeling products include HaloTag® Interchangeable Labeling Technology—a versatile protein analysis tool used to image live or fixed mammalian cells, purify proteins, investigate protein interactions and more. The HiBiT protein tagging system provides a powerful method for monitoring and quantifying proteins at endogenous expression levels with a simple luminescent signal. Learn about Protein Detection Shop Protein Detection products Protein Detection Product Groups Protein Degradation Assays to detect ternary complex formation, ubiquitination, E3 ligase target engagement and subsequent protein degradation by the ubiquitin-proteasome system. Protein Quantification Sensitive bioluminescent-based methods to detect and quantify HiBiT-tagged proteins in cells, on the cell surface or secreted in the medium. Protease Assays Bioluminescent assays to measure the activity of purified cellular proteases and proteasomes. Protein Labeling Cell imaging and in vivo protein localization tools. Includes HaloTag® technology, antibody blocking reagents, and fluorescent and colorimetric labeling systems for immunoblotting. Primary and Secondary Antibodies Antibodies, substrates and accessory products for immunoblotting, immunocytochemistry and immunohistochemistry. Top Protein Detection Products for Your Lab HaloTag® Fluorescent Ligands For protein labeling and detection. Each includes a chloroalkane group for HaloTag® protein attachment. G8251, G8252, G2801, G2802, G8272, G8273, G8581, G8582, G1001, G1002, G8471, G8472, G2991, G3221, G8281, G8282, G8591, G8592 Janelia Fluor® 549 and 646 HaloTag® Ligands Dyes for fluorescent labeling, confocal imaging and high-resolution single-molecule imaging studies of HaloTag® fusion proteins in living cells. GA1110, GA1111, GA1120, GA1121 Nano-Glo® HiBiT Lytic Detection System Bioluminescent method for detecting the total amount of HiBiT-tagged proteins in the cell. N3030, N3040, N3050 Need a product modified? Contact us to discuss custom packaging, formulation and format options for protein analysis products. Learn More Protein Detection Resources Resource Quantify Proteins Tagged with a Luminescent Peptide Webinar Tracking Protein Localization and Movement in Cells Protein Quantitation and Detection Basics The detection and quantitation of individual proteins is one of the fundamental aspects of proteomics. Immunological-based methods such as quantitative enzyme-linked immunosorbent assays (ELISA), Western blotting and dot blotting are very common and sensitive assays for protein detection, and they use antibodies that react specifically with entire proteins or specific epitopes (e.g., fusion tags) after cell lysis. Detection techniques are typically based on chemiluminescence or fluorescence. For live-cell detection of proteins, fluorescence imaging microscopy is now routinely used. The most common and conventional method is the use of intrinsically fluorescent proteins (FPs) related in structure or sequence to green fluorescent protein (GFP). Alternatively, a series of self-labeling enzymes have been developed that can covalently attach a fluorescent ligand to one of its own amino acid residues. These enzymes are similar in size to FPs. If these enzyme domains are fused in frame to a protein, the pair can be labeled by introducing a cell-permeable fluorescent ligand, which covalently reacts with the fusion tag. Related Protein Analysis Products and Resources Categories Mass Spectrometry Protein Expression Protein Interactions Protein Purification Guides and Selectors Protein Expression Protein Purification Let's find the product that meets your needs. Talk to a Scientist Angus UK Don't miss out! Stay notified of Promega events, products and news. Sign Up Need Assistance? Contact Promega Customer and Technical Support Local Contact +31 (0)71 532 42 44 Products & Resources Products Applications Custom Capabilities Resources About Promega Company Info ISO Certification Corporate Responsibility Press Releases Careers © 2021 Promega Corporation, an affiliate of Promega Benelux BV. All rights reserved. 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