PRODUCTS SOLUTIONS SERVICE SCIENCE LAB COMPANY EN CONTACT Home Solutions Life Science Fluorescence Lifetime Imaging Fluorescence Lifetime Imaging Fluorescence lifetime imaging microscopy (FLIM) is an imaging technique that makes use of the inherent properties of fluorescent dyes. Aside from having characteristic emission spectra, each fluorescent molecule has a characteristic lifetime that reflects how long the fluorophore spends in the excited state before emitting a photon. Lifetime analysis provides you with information in addition to your standard fluorescence intensity measurements. Historically, lifetime imaging has been a slow, complex, and specialized technique. Its use was limited to experienced microscopists or physicists. Leica Microsystems is at the cutting edge of today’s fluorescence lifetime imaging advances. Our systems make lifetime imaging faster and easier to use than ever before, binging its advantages to every day confocal imaging experiments. SIMPLY GET IN TOUCH! Our experts on solutions for FLIM are happy to help you with their advice. Boost confocal imaging with fluorescence lifetime information Fluorescence lifetime imaging provides additional information that can help you improve the quality of your confocal imaging. It can be particularly useful to discriminate fluorescence probes that have overlapping fluorescent emission spectra, or to eliminate unwanted background fluorescent signals. FLIM is a crucial application for measuring and quantifying imaging data. As lifetime information is independent of fluorophore concentration, it is extremely well suited for functional imaging. Functional imaging goes beyond the traditional recording of the location and concentration of molecular species and enables further investigation of molecular function, interactions, and their environment. Additionally, FLIM enables novel imaging applications, such as the use of biosensors to study the cell’s microenvironment or imaging based on the intrinsic fluorescent properties of your sample. Learn more about FLIM and its imaging applications with the SP8 FALCON system by referring to this application note. The lifetime contrast of sepal of silverberry allows to separate different structure within the sample. The intensity image (yellow image) doesn't show any specific structure while the FastFLIM (rainbow scale image) and the lifetime fitted images (RGB, three component image) give insigth into the different part of the sample. The end of the video shows the three structures determined by the lifetime fit split into three channels (RGB). Setting the standard for lifetime imaging With the release of the fast and fully integrated lifetime imaging technology – the SP8 FALCON – Leica Microsystems set the benchmark for the future of lifetime imaging. Leica Microsystems received the prestigious R&D 100 Award for the SP8 FALCON system. This award recognizes the solution’s innovative approach to lifetime imaging. Leica Microsystems solutions for FLIM enable you to harness the power of fluorescence lifetime to extract a new dimension of information from every sample with your confocal experiment. Investigate cellular physiology and explore cellular dynamics with FALCON. Get immediate access to functional information such as metabolic status, pH and ion concentration with the potential of Tausense in STELLARIS. With our simplified, integrated platforms, you can gain new perspectives on your experiments, allowing you to harness new answers to your experimental questions. FLIM PRODUCTS 2 Filter by Area of Application Life Science Research Live Cell Imaging Super-Resolution Cancer Research Organoids and 3D Cell Culture Neuroscience Fluorescence Microscopy Fluorescence Lifetime Imaging Cell Biology Virology Model Organisms in Research STELLARIS 5 & STELLARIS 8 With the STELLARIS confocal platform, we have re-imagined confocal microscopy to get you closer to the truth. Live Cell Imaging , Super-Resolution , Cancer Research , Organoids and 3D Cell Culture , Neuroscience , Fluorescence Microscopy , Fluorescence Lifetime Imaging , Cell Biology , Virology , Model Organisms in Research STELLARIS 8 FALCON FLIM Microscope Fluorescence Lifetime Imaging , Live Cell Imaging , Organoids and 3D Cell Culture , Virology RELATED ARTICLES Read our latest articles about Fluorescence Lifetime Imaging The knowledge portal of Leica Microsystems offers scientific research and teaching material on the subjects of microscopy. The content is designed to support beginners, experienced practitioners and scientists alike in their everyday work and experiments. MORE ARTICLES Fluorescence Microscopy How Industrial Applications Benefit from Fluorescence Microscopy Watch this free webinar to know more about what you can do with fluorescence microscopy for industrial applications. We will cover a wide range of investigations where fluorescence contrast offers new… Read article Nov 24, 2021 Webinar 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 Interested to know more? Talk to our experts. We are happy to answer all your questions and concerns. CONTACT US Do you prefer personal consulting? Toggle © 2021 Leica Microsystems Sales & Service Terms and Conditions Imprint Terms of Use Privacy Policy Do not sell my personal data Product Security Cookie Settings Language:English