Products Area of Interest Resources Support Company Careers Chromium Single Cell Immune Profiling Multiomic profiling of immune diversity, cell by cell Chromium Single Cell Immune Profiling provides a multiomic solution to your immunology questions. Analyze full-length, paired B-cell or T-cell receptors, surface protein expression, antigen specificity, and gene expression, all from a single cell. Request Pricing See How It Works Resources: Product Catalog Product Sheet Automation Kit Product Sheet Single Cell Buyer's Guide Multiomic cytometry Highly sensitive detection of 5’ mRNA with antigen specificity and phenotyping. Complete immune repertoire Paired, full-length Ig sequence, including isotypes, and T-cell receptor α/β. Immune mapping Combine immune repertoire with antigen specificity at single cell resolution. Streamlined data analysis Explore and visualize clonotype information with easy-to-use software. One-day lab workflow From sample to sequencing-ready library in one day, with an option for an automated workflow. Scalable solutions From standard experimental needs to higher throughput for comprehensive studies, find the kit that fits your research. Explore what you can do Discover novel cell types and functions Recover a comprehensive immune repertoire Explore immune receptor mapping Discover new biomarkers Flexible solutions to meet your needs Single cell resolution for comprehensive immune profiling Chromium Single Cell Immune Profiling lets you explore the incredible diversity of the innate and adaptive immune system with multiomic readouts and flexibility of scale. Download Product Sheet Multiomic analysis Simultaneously measure cell surface protein expression and antigen specificity with 5’ gene expression. Immune Repertoire ProfilingCell Surface ProteinAntigen Specificity Large-scale projects Tackle your most ambitious projects, from rare cell type discovery to translational studies, with our high-throughput (HT) kit and complementary solutions for larger-scale projects. Single Cell Immune Profiling HTTargeted Gene Expression Workflow automation Maximize lab productivity and obtain consistent, reproducible results with Single Cell Immune Profiling for Chromium Connect. Automated Single Cell Immune Profiling Proven results Videos Watch how customers are using Chromium Single Cell Immune Profiling. View all Publications Resources High Resolution Characterization of the Immune System with Single Cell Immune Profiling v2 Ravi Ramenani, Sarah Taylor, 10x Genomics, July 2020 Antibody Discovery and Immune Profiling Using High-Throughput Single B Cell Sequencing Ian Setliff, PhD, Candidate, Vanderbilt University, Nov 2018 A New Way of Exploring Immunity Mike Stubbington, Alvaro Martinez Barrio and Dagmar Walter, July 2019 View all Our end-to-end solution Chromium instruments with Next GEM technology Chromium X Series Our scalable instrument Chromium Controller Our compact instrument Chromium Connect Our automated instrument Chromium Single Cell Immune Profiling reagents With our reagent kits, explore adaptive immunity through multidimensional approaches, including 5’ mRNA, full-length paired BCR and TCR sequences, antigen-receptor specificity, cell surface proteins, and more. Analysis and visualization software Cell Ranger Our analysis pipelines Loupe Browser Our visualization software World-class technical and customer support Our expert support team can be contacted by phone or email. Workflow The base workflow for Single Cell Immune Profiling is shown below. If you’re using our Feature Barcoding Kit for Cell Surface Protein or Antigen Specificity, we provide additional protocols for modifying your cells of interest. For the automation option, separate reagents and protocols are available for Chromium Connect. 1 Prepare your sample Start with hundreds to hundreds of thousands of cells. Cells can be labeled with antibodies and/or peptide–MHCs conjugated with a unique oligonucleotide barcode. You can use cell suspensions from FACS/MACS, fine needle aspirates, TILs, PBMCs, and more. RESOURCES Single Cell Preparation Guide Demonstrated Protocols 2 Construct your 10x library Construct a 10x barcoded library using our reagent kits and a compatible Chromium instrument. Each member of the Chromium instrument family encapsulates each cell with a 10x barcoded Gel Bead in a single partition. Within each nanoliter-scale partition, cells undergo reverse transcription to generate cDNA, which shares a 10x Barcode with all cDNA from its individual cell of origin. RESOURCES User Guide / Protocol HT User Guide / Protocol Automated User Guide / Protocol 3 Sequence The resulting 10x barcoded library is compatible with standard NGS short-read sequencing on Illumina sequencers, for immune and transcriptional profiling of thousands of individual cells. RESOURCES Sequencer Compatibility List 4 Analyze your data Convert raw sequencing data into biologically meaningful insights with Cell Ranger. Cell Ranger processes data from paired immune receptor repertoire sequences, gene expression, and antigen specificity. ANALYSIS PIPELINES OUTPUT Output includes QC information and files that can be easily used for further analysis in our Loupe Browser visualization software or third-party R or Python tools. RESOURCES Cell Ranger Analysis Pipelines Running Cell Ranger in Cloud Analysis 5 Visualize your data Use our Loupe Browser visualization software to interactively explore your results and compare detailed clonotype information within and across samples and cell types to study gene usage, repertoire diversity, clonal expansion, and more. DO I NEED TO BE A BIOINFORMATICIAN TO USE IT? Loupe is a point-and-click software that’s easy for anyone to download and use. RESOURCES Loupe Visualization Software Downloadable Datasets Frequently asked questions What does Chromium Single Cell Immune Profiling enable you to do? Can I sequence the B- or T-cell receptors without gene expression? What types of samples are compatible? Do I get the full-length variable region? Am I able to isotype immunoglobulin? Related Products View All Products Products Single Cell Immune Profiling View details Single Cell Gene Expression View details Spatial Gene Expression View details Single Cell ATAC View details Capabilities Paired full-length TCR / BCR sequencing 5’ whole transcriptome gene expression Targeted gene expression 3' whole transcriptome gene expression Targeted gene expression Sample multiplexing 3' whole transcriptome gene expression Targeted gene expression Compatible with FFPE and fresh frozen tissue sections Epigenomic landscape by open chromatin profiling Extensions Cell Surface Protein Antigen Specificity Sample multiplexing Cell Surface Protein CRISPR Screening Spatial Gene Expression with Immunofluorescence Immunofluorescence protein co-detection – Instruments Chromium X Series Chromium Controller Chromium Connect Chromium X Series Chromium Controller Chromium Connect – Chromium X Series Chromium Controller Get Started Request Pricing ABOUT Products Platform Company Careers Contact Employment Verification LEGAL NOTICES Privacy Policy Terms of Use Email Preferences Other Legal Notices HEADQUARTERS +1 925 401 7300 +1 800 709 1208 info@10xgenomics.com RESOURCES Publications Support Videos SOCIAL SIGN UP FOR PRODUCT AND EVENT UPDATES Subscribe © 2021 10x Genomics. 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