--> PerkinElmer - Cisbio Home Life sciences / Technology / Immunoassay Technologies to Fit Every Need Immunoassay Platforms to Fit Every Need Be sure to choose the best possible option for success The right technology choice for a successful assay When it comes to choosing the right assay for your research, traditional technology almost always gives you the choice between going with ELISA (enzyme-linked immunosorbent assays) or Western Blot. While these techniques are quite well known and widely used, others can be implemented which will provide you with additional benefits like ease of use, scalability, or the absence of washing, without compromising on sensitivity and specificity. DELFIA®, LANCE®, HTRF®, or Alpha technologies are among the many options to explore! ELISA DELFIA HTRF and LANCE Ultra Alpha PLATE FORMAT 96-wells 96 to 384-wells Flexible: Anywhere from 96- to 1536-wells MINIATURIZATION Limited Limited Yes Yes TIME TO RESULTS 4-24 hr 4-6 hr 1-2 hr 1-2 hr – – Significant time saving reallocated back to science WASH STEPS Yes Yes No No While timely, in some instances wash steps can be beneficial. Wash steps may become necessary to remove nonbound analytes with higher cross-reactivity profiles and to decrease background Absence of wash steps increases throughput, results in ease of use, reduces time to results and lowers variability DYNAMIC RANGE 2 Log 5 Log 4 Log 5 Log – Large dynamic ranges are beneficial to avoid making extremely large dilutions during sample preparation. Large sample dilutions are cumbersome and lead to analytical imprecision/variability SENSITIVITY 10 pg/mL 10 pg/mL 10 pg/mL 1 pg/mL Sufficient sensitivity to assay in serum, fluid, cell supernatant & lysates Sufficient sensitivity to assay in cell supernatant & lysates Sufficient sensitivity to assay in cell supernatant & lysates. May lend itself to diluting then testing serum samples, due to its superior sensitivity AVIDITY No No Only with streptavidin Yes – – Avidity may be a sought after characteristic when detecting protein-protein interactions of low affinities SIGNAL STABILITY Up to 1 hr Days – Years 24 hr to days up to 24 hr – Allows for the plates to be left on the bench and read at an alternate later and more convenient time. Maximizes assay precision when incubation times vary – MULTIPLEXING No Yes (up to 3 targets) No Yes (up to 3 targets) READER REQUIREMENTS Chromogenic and Chemiluminescence Compatible TR-FRET Compatible TR-FRET Compatible Alpha Compatible – 100+ compatible reader 50+ compatible reader 10+ compatible reader CHEMISTRY – Fluorophore Chelate Fluorophore Chelate (LANCE Ultra) and Fluorophore Cryptate (HTRF) Fluorophore Embedded Beads – Extremely robust chemistry, light, temperature insensitive. Ion insensitive (HTRF only) – ANALYTE SIZE No limitation No limitation Subject to förster radius (9 nm) Subject to oxygen singlet diffusion (200 nm) DELFIA Next DELFIA technology DELFIA assay principle DELFIA (dissociation-enhanced lanthanide fluorescence immunoassay) is a time-resolved fluorescence (TRF) intensity technology. DELFIA has a similar assay principle and workflow to that of a traditional ELISA, but with the added benefits of a stable, time-resolved fluorescent signal improving assay dynamic range. DELFIA also offers a multiplexing capability. Learn more about DELFIA How DELFIA compares to ELISA Below is a workflow comparison between a DELFIA TRF assay and a traditional ELISA. PerkinElmer - Cisbio Get on the list to receive remarkable content Subscribe Life sciences In Vitro Diagnostic Cisbio Select your country Follow us