Search HOME PRODUCTS SUPPORT WEBSHOP EVENTS COMPANY 5'-Position Modifications 5'-Phosphate The unmodified synthesis has only 3'- and 5'-hydroxy groups. 5'-Phosphate oligonucleotides for cloning are offered as a 5'-modification to the regular synthesis. 5'-Amino 5'-Amino modification is a primary amine linked to the carbon-5 of the 5'-ribose via a C3, C6, C12 or C2OC2-phosphate-linker. Amino-C4 and -CX are also possible (See “Amino groups and Carboxy-dT” under “Internal or 5'-positions”). Additionally different spacers are possible (See “Spacers” under “Internal or 5'-positions”). Please note that 5'-amino-C3 is not available with HPLC purification. 5'-Biotin The standard coupling of a Biotin to the 5' end of an oligonucleotide is done including a C6 linker, but C4 or our CXS linker is also optional (See “Biotin” under “Internal or 5'-positions”). Additionally, different spacers are possible (See “Spacers” under “Internal or 5' positions”). 5'-Thiol The 5'-Thiol modification is linked to the 5'-carbon of the ribose via a C6-phosphate-linker. 5'-Thiololigonucleotides are delivered dry and trityl-protected. Protocol for removal of the trityl-group prior to use is supplied. The Thiol modification can also be obtained from a disulfide (See “Miscellaneous” under “Internal or 5'-positions”). For more spacing different spacers are possible (See “Spacers” under “Internal or 5'-positions”). 5'-Fluorescent Dyes Please refer to our dye selection chart for available 5’-fluorescent dyes modifications. 5' modification with dyes includes the fluoresceins ABI-dyes HEX, TET, 6-FAM and the VIC analog CAL Fluor® Orange, the Rhodamines TAMRA and ROX, the Texas Red analog CAL Fluor Red 610 and the LC Red 640 analog CAL Fluor Red 635. Dye Abs. max. Em. max. Abs. coef. 6-FAM 494 517 83 TET 522 538 73 HEX 535 553 73 CAL Fluor Orange 560 540 561 94 Quasar® 570 (Cy3 analog) 550 570 150 TAMRA 560 583 89 CRF-610 (Cy3.5 analog) 581 596 150 ROX 587 607 82 Texas Red 596 615 85 CAL Fluor Red 610 590 610 108 CAL Fluor Red 635 616 635 112 Quasar 670 (Cy5 analog) 649 670 250 Quasar 705 (Cy5.5 analog) 678 704 250 Abs. coef.: Molar Extinction Coefficient x 1000 (cm-1M-1) Quick Links Dual-labeled BHQ Probes Dual-labeled BHQplus Probes Oligonucleotides Oligo Ordering Modifications FAQs Price list Questions? Name* Email* Questions/Comments* HOME Sitemap Legal Contact Us Copyright ©2020, LGC Biosearch Technologies +45 87 32 30 00