This site uses functional cookies and external scripts to improve your experience. Privacy policy My settings Accept Member access Global ELITechGroup proprietary technologies Unique Real-Time PCR technologies Products ELITechGroup Proprietary Technologies Molecular Diagnostics Over the years, we have developed a comprehensive suite of PCR technologies, including the revolutionary Minor Groove Binder (MGB), to design superior sensitivity and specificity real-time PCR assays beneficial for laboratories, and clinicians. Request a quote Pleiades probes Pleiades probes are proprietary probes concentrating all ELITechGroup PCR technologies, designed with a Minor Groove Binder (MGB), Eclipse® Dark Quencher, Aquaphluor® dyes, and Superbases® • Very low background and high hybridization-triggered fluorescence • Unique quenching effects with the combined action of MGB and Eclipse® Dark quencher • Exceptional specificity due to a shorter length • Improve sensitivity by combination high hybridization signal with low and stable background • Melt-curve analysis capability Probe MGB MGB is a synthetic molecule attached to the 5’-end of MGB DNA probes, that binds in the minor groove of hybrid DNA molecules • Increase stability of hybrid DNA enabling the design of shorter probes for excellent specificity and sensitivity polymorphisms discrimination • Identify emerging mutations and reliably discriminate clinically important variants • Increase reproducibly in A-T and G-C rich regions • Enable reliable, clear and unambiguous melt-curve analysis Eclipse® DarkQuencher Eclipse® DarkQuencher absorbs the fluorophore energy and dissipates it as heat; while a typical quencher re-emits part of the energy as light • Improve absorbance of fluorescence emitted from dye reagents • Decrease background level of fluorescence • Improve PCR assay sensitivity • Cover 4 spectra wavelengths Aquaphluor® dyes Aquaphluor® dyes are fluorescent compounds that can re-emit light upon light excitation • Comprehensive range of 25 different colours from UV to far-red developed • Perfectly adapted to multiplexed assays Superbases: Super A, Super T, Super G, and Super I Superbases are engineered nucleotides selectively used to optimize primers and probes design • Enhance A-T stability (e.g. rich A/T domains) • Reduces secondary structure (e.g. GG self-association) and fluorescence quenching • Confer close Tm value when paired with any bases Downloads Evergrowing Menu Brochure (EN) ELITe MGB® Catalog Brochure (EN) Already own this product? With the product code you gain access to the technical documents of this product. Instructions for use How can we help you? For general inquiries please fill out the form on the right – for questions about specific products or pricing on products fill out the form on that product page. Someone will be in touch with you soon. Request type General inquiry Product information Service Quote Request to be contacted by our local representative Profile Laboratory Hospital laboratory Supplier Press / Media Other May we send you emails in the future?* Yes No By using this form you agree with the storage and handling of your data by this website. Company About us Contact Careers News Please be aware. This website contains information on products that are targeted to a wide range of audiences and could contain product details or information otherwise not accessible or valid in your country. © 2021 ELITechGroup Privacy Policy Terms of use