Dr. K.K. Sabu Jawaharlal Nehru Tropical Botanic Garden & Research Institute (JNTBGRI) Home About Current Research Recent publications Research Team Friday, July 3, 2015 High quality RNA extraction from small cardamom tissues rich in polysaccharides and polyphenols Abstract Due to the presence of a diverse array of metabolites, no standard method of RNA isolation is available for plants. We noted that polysaccharide and polyphenol contents of cardamom tissues critically hinder the RNA extraction procedure. Hence, we attempted several methods for obtaining intact mRNA and small RNA from various cardamom tissues. It was found that protocols involving a combination of commercial kits and conventional CTAB (cetyl trimethylammonium bromide) methods yielded RNA with good purity, higher yield, and good integrity. The total RNA isolated through this approach was found to be amenable for transcriptome and small RNA analysis through next-generation sequencing platforms. Keywords RNA isolation; Next-generation sequencing (NGS); mRNA; miRNA; RIN value http://dx.doi.org/10.1016/j.ab.2015.05.017 Full paper: http://authors.elsevier.com/a/1RJrWlbACuNf Analytical Biochemistry Fasiludeen Nadiyaa, Narayanannair Anjalia, Appukuttannair Gangaprasadb, Kalluvettankuzhy Krishnannair Sabua, , a Biotechnology and Bioinformatics Division, Jawaharlal Nehru Tropical Botanic Garden & Research Institute (JNTBGRI), Thiruvananthapuram 695562, Kerala, India b Department of Botany, University of Kerala, Thiruvananthapuram 695037, Kerala, India Received 22 May 2015, Accepted 28 May 2015, Available online 3 June 2015 Corresponding author Kalluvettankuzhy Krishnannair Sabu - sabu@jntbgri.res.inBiotechnology and Bioinformatics Division, Jawaharlal Nehru Tropical Botanic Garden & Research Institute (JNTBGRI), Thiruvananthapuram 695562, Kerala, India. Email ThisBlogThis!Share to TwitterShare to FacebookShare to Pinterest Labels: miRNA, mRNA, Next-generation sequencing, NGS, RNA Older Posts Home Subscribe to: Posts (Atom) Search My Blog Loading... Previous Posts Previous Posts July (2) October (1) August (1) May (1) December (1) August (1) July (1) March (1) February (1) January (1) November (1) March (14) Popular Posts PhD Thesis: Intraspecific variations in Andrographis paniculata Nees Abstract: For the biodiversity- rich developing countries in the tropics, chemical and genetic prospecting of their plant genetic resources ... Using SAS® procedures to estimate genetic variances and heritability in a rice cross Abstract Heritability is a measure of possible genetic advancement under selection. Estimation of variance components could imply partitioni... 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Links Jawaharlal Nehru Tropical Botanic Garden & Research Institute (JNTBGRI) Kerala State Council for Science, Technology & Environment (KSCSTE) Kerala University University Kebangsaan Malaysia(UKM) UKM Rice project Prof. Wickneswari Ratnam My PhD Thesis Sabu @ Academia.edu Simple template. Template images by simonox. Powered by Blogger.