Home Resources Product and Technology Guides Learn more about basic and advanced methods for cell biology, nucleic acid analysis, protein analysis and basic research in these educational resources. Each guide provides an overview of the basic science behind the methods and techniques described, as well as links to products and other helpful resources. Cell Biology Introduction to 3D Cell Culture 3D cell cultures are increasingly being used for basic research and drug discovery. Here you'll learn what 3D cell cultures are, why and how they are being used, and how to validate assays that work with 3D models. ATP Assays Learn how ATP assays work and how to choose the right assays for your needs. Cell Line Authentication Resources Misidentified cell lines can lead to lost resources, time and effort. Explore why cell line authentication is important and learn how to confirm the identity of your cells. Choosing a Luciferase Reporter Assay A comparison guide to help you select the right luciferase assay for your needs. Interpreting Multiplex Cell Health Data The CellTox Green Cytotoxicity Assay can be used with other cell health assays to provide richer information about why and how cells die. This interactive guide teaches you how to interpret the results from these experiments. Live-Cell Kinetic Assays for Time Course Analysis Learn about simplified cell-based assays that allow you to repeatedly measure one sample well over time. Assays for Biochemical Targets This selector tool is designed to help you quickly find the right Promega assay for your protein target of interest. Cell Imaging An overview of methods used for imaging studies in cell biology. Reporter Genes and their Applications How to use bioluminescent reporter genes to study eukaryotic gene expression and cellular events. Apoptosis An introduction to apoptotic cell death, including methods to confirm apoptosis. Cell Viability Assay Guide How to choose the right cell viability assay for your needs. Transfection A guide to transfection including protocols and optimization tips. Nucleic Acid Analysis Subcloning Guide A guide to the fundamentals of molecular cloning, including restriction digestion, DNA ligation, vector dephosphorylation, and bacterial transformation. Restriction Enzyme Resource Guide Restriction enzymes 101, including reference information and links to restriction digest tools. DNA Purification An overview of DNA extraction methods for manual and automated purification needs. Epigenetics An introduction to the study of epigenetic changes in gene expression. PCR Amplification An overview of methods for PCR, RT-PCR and qPCR. Lab Equipment and Supplies Buffers for Biochemical Reactions All you need to know about preparing buffers. Fluorophores A guide to Promega Fluorometers and Fluorescence Modules or Optical Kits. Protein Analysis Protease Digestion for Mass Spectrometry A collection of protocols for in-gel and in-solution protein digestion with trypsin, trypsin/lys-c or alternative proteases. Protein Expression An introduction to in vitro transcription and translation methods. Protein Purification An overview of common methods for protein purification. A Proteomics Product Guide An overview of products used for protein expression, purification and analysis. Don't miss out! Stay notified of Promega events, products and news. Sign Up Need Assistance? Contact Promega Customer and Technical Support Local Contact +31 (0)71 532 42 44 Products & Resources Products Applications Custom Capabilities Resources About Promega Company Info ISO Certification Corporate Responsibility Press Releases Careers © 2021 Promega Corporation, an affiliate of Promega Benelux BV. All rights reserved. Legal and Trademarks Privacy Policy Terms and Conditions Trademarks Promotional Terms and Conditions Terms and Conditions of Sale and Services Manage Cookie Consent English – Belgium Promega's Cookie Policy Close We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Some of these cookies are essential for our website to work. For others, we won’t set them unless you accept them. To find out more about cookies and how to manage cookies, read our Cookie Policy. If you are located in the EEA , the United Kingdom, or Switzerland, you can change your settings at any time by clicking Manage Cookie Consent in the footer of our website. Manage My Cookies Reject All Cookies Yes, I Accept