Glucagon ELISA (10-1271-01) Kit format: 1 x 96 wells Measurement range: 1.5 – 130 pmol/L (5 – 453 pg/mL) Sample volume: 25 µL No purification of samples Sample types: Serum, EDTA Plasma, and cell-culture medium Measurement range: 1.5 - 130 pmol/L (5.95 - 453pg/mL) Detection limit: 0.75 pmol/L (2.61 pg/mL) No cross-reactivity to glicentin, proglucagon 1-16, and glucagon 3-29 Highly appreciated and often cited assay CE/IVD labeled on select markets - 000 + View in cart Contact us via e-mail Description The Mercodia Glucagon ELISA is a CE/IVD-labelled, highly specific and sensitive immunoassay for measuring glucagon in plasma and serum. A revolutionizing ELISA for sensitive and specific determination of glucagon in human samples. A research tool advancement that allows science to take the next step. The highly specific monoclonal antibodies used in this assay enable accurate determination of glucagon without significant cross-reactivity to other circulating pro-glucagon-derived peptides, e.g. glicentin. Mercodia Glucagon ELISA was launched in 2013 and has since contributed to advancing research by, for the first time, allowing specific and sensitive measurements of glucagon. A few years later, a glicentin ELISA was launched that made it possible to accurately measure circulating glicentin, and with help of this assay, it was shown that levels of glicentin are increasing during an OGTT in gastrectomy patients to levels not seen in any patient group before. Levels of oxyntomodulin are also increasing, but not to the same extent. When such high levels of glicentin appear in the circulation, also a low cross-reactivity will disturb the measurement of glucagon and give rise to falsely high levels. After discussions with different research groups, a sequential protocol that removes the small amount of cross-reactivity to glicentin was developed. As of October 1st, 2020, the assay protocol of Mercodia Glucagon ELISA 10-1271-01 is updated to make the sequential protocol default, abolishing cross-reactivity to glicentin. To run the old, simultaneous protocol, please refer to Technical Note TN34-161. Validated in human. For other species, please visit the Mercodia Glucagon ELISA 10µL product page Test characteristics Samples: Serum, EDTA plasma and cell culture medium. Sample volume: 25 µL Assay range: 1.5-130 pmol/L (5.95-453pg/mL) Detection limit: 0.75 pmol/L Incubation: 18-22 h (overnight) + 1 h, 30 min Detection method: absorbance Specificity The following cross-reactions have been tested: Glicentin n.d Oxyntomodulin 4.0 % Proglucagon 1-61 n.d Glucagon 3-29 n.d Directions for use Version 12.0: Lot.nr 32229, 31792, 32837 For other languages and older versions scroll down to All related documents. Technical notes Simultaneous protocol for Glucagon ELISA Sample collection for Mercodia Glucagon Instruction for manual wash Troubleshooting Instruction for producing additional calibrators Sample types and buffer compatibility Related material Material Safety Data Sheet EN An appreciated assay used in many studies - Bibliography Glucagon Assay Guide Related products Glucagon Control – Low, Medium, High Glucagon ELISA - 10 µL Glicentin ELISA References Kahn, S.E. et al. (2021). Hyperglucagonemia Does Not Explain the β-Cell Hyperresponsiveness and Insulin Resistance in Dysglycemic Youth Compared With Adults: Lessons From the RISE Study. Diabetes Care, Jun; dc210460. Zhu, X. et al. (2021). SGLT2i increased the plasma fasting glucagon level in patients with diabetes: A meta-analysis. European Journal of Pharmacology, 903, 174145 Bevacqua, R. J. et al. (2021). CRISPR-based genome editing in primary human pancreatic islet cells. Nature Communications, 12(1), 2397. Alexiadou, K. et al. (2020). Proglucagon peptide secretion profiles in type 2 diabetes before and after bariatric surgery: 1-year prospective study. BMJ open diabetes research & care, 8(1), e001076. Stern, J. H. et al. (2019) Obesity dysregulates fasting-induced changes in glucagon secretion. J. Endocrinol. 243(2), 149–1606. Roberts GP et al (2018) Gastrectomy with Roux-en-Y reconstruction as a lean model of bariatric surgery. Surg Obes Relat Dis May;14(5):562-5682. Gar, C. et al. (2018) Patterns of plasma glucagon dynamics do not match metabolic phenotypes in young women. J. Clin. Endocrinol. Metab. 103, 972–982. Raffort, J. et al. (2017) Fasting Circulating Glicentin Increases After Bariatric Surgery. Obes Surg 27:1581–1588. Miyachi, A. et al. (2017) Accurate analytical method for human plasma glucagon levels using liquid chromatography-high resolution mass spectrometry: comparison with commercially available immunoassays. Anal. Bioanal. Chem. 409, 5911–5918. Wewer Albrechtsen, N. J. et al. (2014) Hyperglucagonaemia analysed by glucagon sandwich ELISA: nonspecific interference or truly elevated levels? Diabetologia 57, 1919–1926. All related documents (76) Welcome to get in touch with us! 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