Patent Document ID: 8492163
Application ID: 13018156
Patent Flag: 1

Claim One:
1. A labeling reagent for use in mass spectrometry, represented by formula I; including a salt form and/or hydrate form thereof; wherein, Y is a 5, 6 or 7 membered heterocyclic ring that may be substituted or unsubstituted and that may optionally be cleavably linked to a support, wherein the heterocyclic ring comprises at least one ring nitrogen atom that is linked through a covalent bond to the group J; J is a group represented by formula —CJ′ 2 -, wherein each J′ is, independently of the other, hydrogen, deuterium, fluorine, chlorine, bromine, iodine, —R 3 , —OR 3 , —SR 3 , —R 3 ′OR 3 or —R 3 ′SR 3 ; K is a group represented by formula —(CK′ 2 ) n — or —((CK′ 2 ) m —X 2 —(CK′ 2 ) m ) p , wherein n is 0 or an integer from 2 to 10, each in is, independently of the other, an integer from 1 to 5, p is an integer from 1 to 4 and each K′ is, independently of the other, hydrogen, deuterium, fluorine, chlorine, bromine, iodine, —R 4 , —OR 4 , —SR 4 , —R 4 ′OR 4 or —R 4 ′SR 4 ; either 1) R 1 is hydrogen, deuterium or R 6 and R 2 is hydrogen, deuterium or R 7 ; or 2) R 1 and R 2 taken together is a group represented by formula —(CR′ 2 ) q — or —((CR′ 2 ) m —X 2 —(CR′ 2 ) m ) p — that forms a ring that bridges the two nitrogen atoms, wherein q is an integer from 1 to 10, each in is, independently of the other, an integer from 1 to 5, p is an integer from 1 to 4 and each R′ is, independently of the other, hydrogen, deuterium, fluorine, chlorine, bromine, iodine, —R 5 , —OR 5 , —SR 5 , —R 5 ′OR 5 or —R 5 ′SR 5 ; X 1 is ═O, ═S, ═NH or ═NR 7 ; each X 2 is, independently of the other, —O— or —S—; and Z is hydrogen or a covalently linked analyte; wherein, each R 3 , R 4 , R 5 , R 6 , and/or R 7 is, independently of the other, alkyl, alkenyl, alkynyl, aryl, heteroaryl or arylalkyl; each R 3 ′, R 4 ′, and/or R 5 ′ is, independently of the other, alkylene, alkenylene, alkynylene, arylene or alkylarylene; and the labeling reagent comprises at least one isotopically enriched site.