Patent Document ID: 20100120098
Application ID: 12605337
Patent Flag: 0

Claim One:
1. A method of tagging a fragment of a target DNA, comprising: i) incubating target DNA with a transposase and a transposon end or transposon end composition comprising a transferred strand comprising a tag domain and a 3′ portion comprising a transferred strand sequence, under conditions wherein a transposition reaction is catalyzed by said transposase, wherein: a) said target DNA is fragmented; and b) said transferred strand of said transposon end or transposon end composition is joined to a 5′ end of a fragment of said target DNA to produce a 5′-tagged target DNA fragment; and ii) incubating said 5′ tagged target DNA fragment with a nucleic acid modifying enzyme under conditions wherein a 3′ tag is joined to a 3′ end of said 5′ tagged target DNA fragment to produce a di-tagged target DNA fragment.