Patent Document ID: 9803174
Application ID: 13558078
Patent Flag: 1

Claim One:
1. A method of producing a culture comprising dopaminergic neurons, comprising: a) initiating differentiation of human embryonic stem (hES) cells; b) culturing the initiated cells from a) in a medium comprising basic fibroblast growth factor (bFGF), optionally further comprising one or more factors selected from neurotrophin-3 (NT-3), brain-derived neurotrophic factor (BDNF), sonic hedgehog (SHH) and fibroblast growth factor 8(FGF8) for a first culture period; and c) culturing the cells from b) in a medium comprising neurotrophin-3 (NT-3) and brain-derived neurotrophic factor (BDNF), or alternatively comprising cyclic adenosine monophosphate (cAMP) and ascorbic acid for a second culture period sufficient to produce a culture comprising dopaminergic neurons.