Patent Document ID: 9193984
Application ID: 13733687
Patent Flag: 1

Claim One:
1. A method for detecting interactions of a bait and a prey comprising the steps of: (a) preparing a cell comprising (i) a first construct comprising a bait, a first labeling material and a translocation module; and (ii) a second construct comprising a prey and a second labeling material; (b) allowing the bait of the first construct and the prey of the second construct to interact; (c) treating with a signaling material to translocate the first construct to the plasma membrane of the cell; and (d) detecting the translocation of the first construct and the second construct to the plasma membrane of the cell in the cell, wherein the translocation module is protein kinase C or its variants, wherein protein kinase C or its variants comprise an amino acid sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5 and SEQ ID NO:7.