Patent Document ID: 8995740
Application ID: 13865036
Patent Flag: 1

Claim One:
1. A computer-implemented method for performing multi-channel hierarchical image segmentation analysis of tissue types, cell units and sub-cellular units in biological tissue, the method comprising: accessing image data corresponding to multi-channel multiplexed image of biological tissue sequentially fluorescent stained to manifest expression levels of a plurality of morphological biomarkers in the biological tissue; performing multi-channel image segmentation analysis on the image data based on the biomarker expression levels to identify locations and configurations of one or more cells in the biological tissue; performing multi-channel image segmentation analysis on the image data based on the biomarker expression levels to identify locations and configurations of one or more sub-cellular morphological units within the one or more cells, wherein; the one or more sub-cellular morphological units include a membrane or a cytoplasm, and the image segmentation analysis identifies the membrane or cytoplasm by using a probability map to generate a ring-like structure modeling the membrane or the cytoplasm; and rendering, on a visual display device, a hierarchical representation of the one or more cells and the one or more sub-cellular morphological units in the one or more cells.