Patent Document ID: 20090272889
Application ID: 12405650
Patent Flag: 0

Claim One:
1. A method of calibrating a spectrophotometer, the method comprising: (a) harvesting rumen fluid from at least one ruminant animal; (b) combining the rumen fluid with a primer composition comprising a carbohydrate; (c) incubating the rumen fluid and carbohydrate of step (b) until a pre-determined endpoint is achieved, thereby yielding primed rumen fluid; (d) digesting a plant matter sample with the rumen fluid of step (c) to yield a digested sample and measuring fiber content of the digested sample; (e) measuring absorbance or reflectance of the plant matter sample of step (d) using a spectrophotometer; and then (f) calibrating the spectrophotometer by correlating the measured fiber content from step (d) with the measured absorbance or reflectance from step (e).