Patent Document ID: 20130280712
Application ID: 13865316
Patent Flag: 0

Claim One:
1. A method of detecting the presence or absence of a target nucleic acid in a test sample comprising: (a) inputting into a learning statistical classifier system data from a training set of samples where the amount of the target nucleic acid and a control nucleic acid is known, (b) using the learning statistical classifier system, calculating a plurality of weights for a general linear classifier; (c) building a general linear classifier with the plurality of weights calculated by the learning statistical classifier system in step (b); (d) contacting the test sample with a reaction mixture containing reagents necessary to amplify the target and the control nucleic acids by polymerase chain reaction (PCR) under conditions enabling PCR; (e) measuring at least one amplification-dependent parameter for the target and the control nucleic acids to obtain a test set of data; (f) applying the general linear classifier built in step (c) to the data obtained in step (e) to classify the test sample as containing or not containing the target nucleic acid, thereby detecting the presence or absence of the target nucleic acid in the test sample.