Patent Document ID: 9920315
Application ID: 14996613
Patent Flag: 1

Claim One:
1. A method comprising: a) fabricating a distributor chip comprising: a plurality of microfluidics channels, each microfluidics channel having an input port on a first surface and a plurality of output ports on a second surface opposite the first surface, each microfluidics channel, its input port and its plurality of output ports laying on a plane parallel to a subsequent or previous microfluidics channel, thereby obtaining parallel rows of the output ports on the second surface; b) fabricating a first overlay comprising parallel rows of microfluidics wells having positions corresponding to the parallel rows of the output ports; c) fabricating a receiver chip comprising parallel rows of microfluidics conduits having positions corresponding to the parallel rows of the microfluidics wells of the first overlay; d) fabricating a stopper layer permeable to air and impermeable to water; e) providing a support layer comprising parallel rows of openings corresponding to the parallel rows of the microfluidics conduits of the receiver chip; f) positioning the distributor chip on the first overlay, the first overlay on the receiver chip, the receiver chip on the stopper layer, and the stopper layer on the support layer; g) aligning the distributor chip, the first overlay, the receiver chip, the stopper layer and the support layer, by aligning the parallel rows of the output ports, the parallel rows of the microfluidics wells, the parallel rows of the microfluidics conduits, and the parallel rows of openings, thereby creating an aligned assembly; h) inserting a solution in each input port of each microfluidics channel of the plurality of microfluidics channels, each solution comprising same reagents and a different barcode for each input port; and j) removing the first overlay from the aligned assembly, thereby obtaining parallel rows of microfluidics wells, each row having the same reagents and a unique barcode in each row of the parallel rows of microfluidics wells of the first overlay.