Patent Document ID: 9248152
Application ID: 13878346
Patent Flag: 1

Claim One:
1. A method for treating Alzheimer's disease, comprising: administering to a subject suffering from Alzheimer's disease, wherein the subiect exhibits a decreased expression of acetylcholine, a therapeutically effective amount of a pharmaceutical composition comprising transfected HB1.F3 human neural stem cells (NSCs) in a manner that the transfected NSCs stably express human choline acetyltransferase (ChAT) in a brain tissue of the subject, wherein the transfected HB1.F3 human NSCs are transfected with a retroviral vector comprising a polynucleotide encoding human ChAT including the amino acid sequence of SEQ ID NO:1, a human cytomegalovirus (CMV) promoter which is operatively linked to the polynucleotide encoding human ChAT, and a SV 40 polyadenylation signal sequence, wherein the NSCs are immortalized by an introduction of a retroviral vector containing v-myc oncogene, and wherein the method restores the acetylcholine level of the subject comparable to a normal acetylcholine level and improves learning and memory function of the subiect when compared to a subject that was administered with non-transfected HB1.F3 NSCs.