Patent Document ID: 8338176
Application ID: 12182050
Patent Flag: 1

Claim One:
1. A method of deriving a homogeneous population of human neural stem cells (NSCs) comprising the steps of: (a) culturing human embryonic stem cells (ESCs) as spheres in serum-free media comprising epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), and leukemia inhibitory factor (LIF); (b) selecting and plating the spheres in a cell culture container in serum-free media comprising EGF, bFGF, and LIF until the spheres attach to the container and the cells form clusters; and (c) selecting and plating the clusters for a number of passages sufficient to change the adherent properties of the cells, wherein the cells grow as an adherent monolayer of nestin positive, homogenous, self-renewing cells.