Patent Document ID: 20050125850
Application ID: 10727399
Patent Flag: 0

Claim One:
1. A transgenic mouse comprising a somatic cell, comprising: (a) in a first chromosome of a chromosome pair, a polynucleotide comprising a promoter operably linked to a chimeric sequence encoding an N-terminal portion of a first marker and a C-terminal portion of a second marker separated by a recombinase target site; (b) at a homologous location of a second chromosome of the chromosome pair, a polynucleotide comprising a promoter operably linked to a chimeric sequence encoding an N-terminal portion of the second marker and a C-terminal portion of the first marker separated by a recombinase target site; and (c) a recombinase functionally expressed by the cell, and which promotes recombination between the target sites of the first and second chromosomes; wherein recombinase-promoted somatic mitotic recombination between the target sites yields alternative pairs of X- or Z-segregated progeny cells, wherein the X-segregated progeny cells comprise a first progeny cell comprising the first chromosome, and a recombined variant of the second chromosome comprising the promoter operably linked to a sequence encoding the N- and C-terminal portions of the first marker; and a second progeny cell comprising the second chromosome, and a recombined variant of the first chromosome comprising the promoter operably linked to a sequence encoding the N- and C-terminal portions of the second marker, and wherein the Z-segregated progeny cells comprise a first progeny cell comprising the first chromosome and the second chromosome, and a second progeny cell comprising a recombined variant of the first chromosome comprising the promoter operably linked to a sequence encoding the N- and C-terminal portions of the first marker; and a recombined variant of the second chromosome comprising the promoter operably linked to a sequence encoding the N- and C-terminal portions of the second marker, wherein the first X-segregated progeny cell produces a first marker-specific signal, the second X-segregated progeny cell produces a second marker-specific signal, the first Z-segregated progeny cell produces neither a first nor second marker-specific signal, and the second Z-segregated progeny cell produces both a first and a second marker specific-signal.