Patent Document ID: 7920994
Application ID: 11584368
Patent Flag: 1

Claim One:
1. A method for achieving an optimal function of a biochemical reaction network in an eukaryotic cell comprising: (a) calculating optimal properties of a biochemical reaction network by applying a computational optimization method to a list of reactions representing said biochemical reaction network; (b) altering said list of reactions in the biochemical reaction network and re-computing the optimal properties; (c) repeating (b) until a desired optimal function is reached; (d) constructing an eukaryotic cell having the genetic makeup containing the biochemical reactions which result from (c); (e) placing the eukaryotic cell constructed under (d) in culture under a specified environment to obtain a population of eukaryotic cells; and (f) cultivating the eukaryotic cells as in step (e) for a sufficient period of time and under conditions to allow the cells to evolve to the desired optimal function determined under (c), wherein the biochemical reaction network comprises a comprehensive biochemical reaction network.