Patent Document ID: 20120045841
Application ID: 13214357
Patent Flag: 0

Claim One:
1. A method of analyzing the effect of a parameter on the stability of a protein, comprising: providing a first solution with a first value of said parameter and a predetermined minimum amount of chemical denaturant; and a second solution having a second value of said parameter and a predetermined minimum amount of chemical denaturant; combining said first solution and said second solution in various ratios to create a plurality of third solutions, each having a desired amount of said parameter and a predetermined minimum amount of chemical denaturant; mixing said each of said third solutions with different amounts of said chemical denaturant to create solutions having different molarities of said chemical denaturant; measuring an observable property of each of said plurality of samples; and creating a plurality of denaturation graphs based on said observable property and said molarity, where each denaturation graph has a different amount of said parameter.