Patent Document ID: 4596035
Application ID: 06508448
Patent Flag: 1

Claim One:
1. A method for transforming forward light scatter and right angle light scatter parameter measurements into a combined one dimensional parameter for quantifying cellular subclasses of a sample comprising: (a) passing the cells, to be differentiated into subpopulations and counted, through a zone illuminated by a focused light source, substantially one at a time; (b) detecting light scattered by the passage of said cells through said zone in a substantially forward angle direction; (c) further detecting light scattered by the passage of said cells through said zone at a substantially right angle direction whereby each cell may be represented by a forward angle light scatter and a right angle light scatter measurement; (d) formulating a trajectory for connecting the cells represented by said forward angle and right angle light scatter measurements; (e) determining a series of lines orthogonal to said trajectory describable by a nonlinear function; and (f) counting the cells along each of said orthogonal lines whereby cellular subpopulations may be enumerated.