Patent Document ID: 7943304
Application ID: 11034085
Patent Flag: 1

Claim One:
1. A method for generating an interphase chromosome profile comprising: obtaining a sample containing cells having at least one interphase chromosome for profiling; obtaining a plurality of different species specific, chromosome specific, and location specific DNA probes, wherein said DNA probes contain different antibody-specific labels and are capable of marking a normal control chromosome of the at least one interphase chromosome for profiling at substantially equidistant locations on the normal control chromosome; hybridizing said sample with said DNA probes; detecting the hybridization of said DNA probes to the at least one interphase chromosome for profiling using a plurality of different specific enzymes, a plurality of different specific antibodies, and different specific substrates, wherein said plurality of different specific enzymes and said plurality of different specific antibodies form a plurality of different specific enzyme-antibody conjugates, each of said enzyme-antibody conjugates comprises at least one of said specific antibodies and at least one of said specific enzymes and wherein each of said specific antibodies binds to one of said antibody specific labels that is attached to one of said specific DNA probes and wherein each of said specific enzymes reacts with its specific substrate from said specific substrates such that mixed differential major color bands are produced on the at least one interphase chromosome for profiling; and comparing said mixed differential major color bands on the at least one interphase chromosome for profiling with mixed differential major color bands on the normal control chromosome by visual analysis and determining the interphase chromosome profile of the at least one interphase chromosome for profiling, wherein said mixed differential major color bands on said normal control chromosome are generated by hybridizing said normal control chromosome with said DNA probes and detecting the hybridization of said DNA probes to said normal control chromosome using said enzyme-antibody conjugates and said specific substrates, and each adjacent band of said mixed differential major color bands on said normal control chromosome are spaced at substantially the same distance.