Patent Document ID: 8412459
Application ID: 12443639
Patent Flag: 1

Claim One:
1. A method for determining the sensitizing potential of a chemical compound, comprising the steps of: (a) providing a suitable cell culture of a specific cell type and providing a test sample and a control sample thereof, said test sample and said control sample being identical, (b) exposing said test sample to a chemical compound in a solvent and exposing said control sample to said solvent for a predetermined period of time, (c) determining for the test sample and the control sample gene expressions for a subset of at least 2 genes selected from the group of genes consisting of SEQ ID NOs: 1 to 29 and SEQ ID Nos: 31 to 45 respectively, (d) for this subset of at least 2 genes looking up in a database the gene expressions for a set of control and test samples, the test samples being exposed for said predetermined period of time to a set of chemical sensitizing model compounds comprising both sensitizers and non-sensitizers, (e) using the gene expressions of the said test sample and the said control sample of step (c) as input to a statistical classification model that is based on said database and that is trained and optimized to classify chemical compounds as either sensitizers or non-sensitizers using gene expressions for said subset of at least 2 genes, and (f) predicting through said model whether the chemical compound tested belongs to the class of sensitizers or to the class of non-sensitizers.