Patent Document ID: 9284562
Application ID: 13512453
Patent Flag: 1

Claim One:
1. A biological circuit chemotactic converter composition comprising: at least one input module (IM) n nucleic acid comprising an inducible promoter nucleic acid sequence (iP A ) operably linked to a repressor nucleic acid sequence encoding a repressor protein (R A ); at least one genetic toggle switch (T S ) n nucleic acid comprising a first repressible promoter nucleic acid sequence (rP 1 ) that drives expression of a second repressor nucleic acid sequence (R 2 ) encoding a second repressor protein, and a second repressible promoter nucleic acid sequence (rP 2 ) that drives expression of a first repressor nucleic acid sequence (R 1 ) encoding a first repressor protein, (rP 1 -R 2 and rP 2 -R 1 ), wherein the rP 1 is inhibited by the first repressor protein and the rP 2 is inhibited by the second repressor protein and wherein R A of at least one input module acts as a repressor for rP 1 of at least one genetic toggle switch; at least one logic module (L M ) n nucleic acid consisting essentially of a repressible promoter nucleic acid sequence (rP B ) operably linked to a repressor nucleic acid sequence (R B ), wherein rP B is inhibited by the second repressor protein of at least one genetic toggle switch; and at least two sensor module (SM A and SM B ) n+1 nucleic acids, wherein each of the two sensor module nucleic acids comprises a repressible promoter nucleic acid sequence (rP C and rP D , respectively) operably linked to a nucleic acid sequence encoding a sensor molecule (sensor A and sensor B) and wherein n ≧1; wherein the repressible promoter nucleic acid sequence of at least one sensor module is repressed by the repressor protein encoded by at least one logic module, and the repressible promoter nucleic acid sequence of another of the at least two sensor modules is directly repressed by the second repressor protein encoded by at least one genetic toggle switch; and wherein if no input signal is received by the at least one input module nucleic acids, only sensor A is expressed, and wherein if an input signal is received by at least one input module nucleic acid, then sensor A is not expressed; and wherein the repressor proteins encoded by any of the logic module nucleic acid sequences are different from the repressor proteins encoded by any of the at least one input module nucleic acid sequences and are different from the repressor proteins encoded by any of the at least one genetic toggle switch nucleic acid sequences.