Patent Document ID: 20130316342
Application ID: 13849452
Patent Flag: 0

Claim One:
1. A method for uniquely labeling a gene of interest in an individual cell nuclei, comprising the steps of: (a) obtaining and providing a single cell from a patient; (b) treating the single cell and fixing it to a substrate for analysis to increase accessibility of a target chromosome and to reduce nonspecific binding; (c) providing a set of three probes to uniquely label the gene of interest in a target chromosome, wherein a first probe is selected to hybridize to the proximal region of the gene on the target chromosome, the second probe is selected to hybridize to the gene of interest, and the third probe is selected to hybridize to the distal region of the gene of interest on the target chromosome, wherein the combination of the three probes provides a unique label for the gene of interest in the individual cell nuclei.