Patent Document ID: 9062336
Application ID: 13789022
Patent Flag: 1

Claim One:
1. A process for amplifying the two complementary DNA strands in a DNA target duplex, wherein said process comprises: (a) contacting in buffered aqueous solution said DNA target duplex with a Basic Reaction Pellet comprising a polymerase, a recombinase, and all other components necessary to support a Recombinase Polymerase Amplification, together with (b) a solution containing any 2′-deoxynucleoside triphosphates that are required to complement each and every nucleotide in the said complementary DNA sequences and substituted primers, where (c) said substituted primers comprise a first primer that is substantially complementary in sequence to a segment at or near the 3′-end of the first of said DNA strands, and a second primer that is substantially complementary in sequence to a segment at or near the 3′-end of the second of said DNA strands, and where (d) within said substituted primer at least one adenine in at least one of its adenosine nucleotides is substituted by 2-aminopurine or diaminopurine, at least one guanine in at least one of its guanosine nucleotides is substituted by inosine, at least one thymine is substituted by 2-thiothymine, and at least one cytosine is substituted by N4-ethylcytosine, and wherein (e) the total number of said substitutions is at least four.