Patent Document ID: 8574831
Application ID: 11933747
Patent Flag: 1

Claim One:
1. A method of evaluating the effectiveness of a protease inhibitor as an antiviral therapy of an HIV-infected patient, comprising: (a) collecting a sample from an HIV-infected patient; (b) amplifying viral genomic sequences using primers targeting the HIV protease gene and determining whether the sample comprises a nucleic acid encoding a HIV protease having the mutation I84C combined with at least one additional mutation selected from L10I, K20R, K20T, L24I, L33F, L33I, M36I, M46L, A71T, A71V, V77I, V82I, or L90M, wherein the codon position number is compared to the sequence of reference wild-type HIV IIIB/LAI; and (c) using the presence of said at least one mutations of step b) to evaluate the effectiveness of said antiviral therapy.