Patent Document ID: 8170852
Application ID: 12769555
Patent Flag: 1

Claim One:
1. A method for making a data structure relating a plurality of Saccharomyces cerevisiae reactants to a plurality of Saccharomyces cerevisiae reactions in a computer readable storage medium or media, comprising: (a) storing in a computer readable storage medium or media a data structure relating a plurality of Saccharomyces cerevisiae reactants to a plurality of Saccharomyces cerevisiae reactions, wherein each of said Saccharomyces cerevisiae reactions comprises a reactant identified as a substrate of the reaction, a reactant identified as a product of the reaction and a stoichiometric coefficient relating said substrate and said product; (b) storing in the computer readable storage medium or media a constraint set for said plurality of Saccharomyces cerevisiae reactions; (c) storing in the computer readable storage medium or media an objective function; (d) adding a metabolic reaction to said stored data structure to obtain a revised data structure and storing the revised data structure in the computer readable storage medium or media; (e) executing commands in a suitably programmed computer using said stored revised data structure, said stored constraint set, and said stored objective function for determining at least one flux distribution for the reactions related in said revised data structure that minimizes or maximizes said objective function when said constraint set is applied to said revised data structure; and (f) visually displaying said at least one flux distribution to a user.