Patent Document ID: 10127350
Application ID: 14790810
Patent Flag: 1

Claim One:
1. A method of identifying a first peak signal corresponding to a first part of a biological sample from among other peak signals of other parts of the biological sample, the biological sample including a plurality of droplets that contain nucleic acids and correspond to different parts of the biological sample, the method comprising, at a computer system: receiving a data signal from a droplet digital polymerase chain reaction (ddPCR) experiment involving the biological sample, the data signal having an amplitude that varies over time, wherein the data signal comprises a plurality of overlapped peaks at different times; transforming the data signal to obtain a two-dimensional array of coefficients corresponding to localized functions, each coefficient corresponding to a localized function having a time location and a scale factor; identifying a first time region corresponding to a first peak of the first peak signal; removing the coefficients of the two-dimensional array corresponding to localized functions with time locations within the first time region; determining new coefficients for the two-dimensional array using a cut function that models tails of other peak signals; using the new coefficients to obtain an altered two-dimensional array; performing an inverse transform of the altered two-dimensional array to obtain a background signal; subtracting the background signal from the data signal to obtain the first peak signal corresponding to the first part of the biological sample; and determining a presence or absence of a nucleic acid sequence in the first part of the biological sample based on the first peak signal.