Patent Document ID: 9556479
Application ID: 14538653
Patent Flag: 1

Claim One:
1. A method of sequencing a DNA template, the method comprising: a) providing a reaction mixture comprising: the DNA template, a replication initiating moiety that complexes with or is integral to the template, a recombinant DNA polymerase capable of replicating at least a portion of the template using the moiety in a template-dependent polymerization reaction, wherein the recombinant polymerase comprises an amino acid sequence that is at least 80% identical to SEQ ID NO:1 and comprises an amino acid substitution at position 375 and an amino acid substitution at position 512, wherein numbering of positions is relative to SEQ ID NO:1, wherein the recombinant polymerase exhibits polymerase activity, and one or more nucleotide analogues, each of which nucleotide analogues is labeled on a phosphate group, wherein the base moiety of each of the analogues is selected from the group consisting of adenine, thymine, guanine, and cytidine; b) subjecting the reaction mixture to a polymerization reaction in which the recombinant polymerase replicates at least a portion of the template in a template-dependent manner, whereby the one or more nucleotide analogues are incorporated into the resulting DNA; and c) identifying a time sequence of incorporation of the one or more nucleotide analogues into the resulting DNA.