Patent Document ID: 9567608
Application ID: 14632497
Patent Flag: 1

Claim One:
1. A method of introducing a nucleic acid of interest into a locus of interest in a chromosome of a cell, said method comprising introducing into said cell: (1) a plasmid comprising: a) a zinc finger nuclease (ZFN) site comprising, in a 5′ to 3′ direction, a first half binding ZFN site, a spacer, a second half-ZFN binding site; and b) said nucleic acid of interest; and (2) a heterodimeric ZFN capable of cleaving said ZFN site; wherein: said locus of interest comprises a nucleic acid comprising, in a 5′ to 3′ direction, said second half ZFN binding site, said spacer, and said first half ZFN binding site, and said introduction of (1) and (2) results in insertion of said nucleic acid of interest into said chromosome of said cell, and wherein upon said insertion, a zinc finder nuclease site comprising said first half binding ZFN site and said second half binding ZFN site is not reconstituted.