Patent Document ID: 9274128
Application ID: 14484846
Patent Flag: 1

Claim One:
1. A method to diagnose Parkinson's disease in a subject comprising the steps of: extracting nucleic acid from a test sample obtained from the subject; measuring an amount of a component in a test sample obtained from the subject, wherein the component is selected from the group consisting of Parkin Interacting Substrate (PARIS), a metabolite of Parkin interacting Substrate (PARIS), an mRNA coding for Parkin Interacting Substrate (PARIS), peroxisome proliferator-activated receptor gamma coactivator 1-α, (PGC-1α), and an mRNA coding for PGC-1α, using at least one primer pair selected from the group consisting of: (a) a first primer pair consisting of a reverse primer consisting of SEQ ID NO: 120 and a forward primer consisting of SEQ ID NO: 119; (b) a second primer pair consisting of a reverse primer consisting of SEQ ID NO: 32 and a forward primer consisting of SEQ ID NO: 31; and (c) a third primer pair consisting of a reverse primer consisting of SEQ ID NO: 76 and a forward primer consisting of SEQ ID NO: 75; determining the expression of the component; and comparing the amount of the component against a baseline expression value to determine whether the levels of PARIS, metabolite of PARIS, an mRNA coding for PARIS are elevated or to determine whether the levels of PGC-1α, or an mRNA coding for PGC-1α are reduced as compared to levels of a healthy subject.