Patent Document ID: 6159688
Application ID: 09040698
Patent Flag: 1

Claim One:
1. A method for generating recombined polynucleotide sequences encoding functionally similar polypeptides, comprising: (a) identifying at least one conserved region in a known polynucleotide encoding a functional polypeptide of interest; (b) designing at least one set of primers directed to the at least one conserved region(s), wherein a set of primers comprises a sense primer and an antisense primer; (c) contacting the at least one set of primers of step (b) with DNA from a first population of polynucleotide sequences encoding the functional polypeptide of interest under conditions wherein the primers anneal and extension sequences are generated; (d) contacting DNA from a second population of polynucleotide sequences encoding the functional polypeptide of interest with the extension sequences generated in step (c) wherein, the extension sequences generated in step (c) anneal to DNA from the second population of polynucleotide sequences encoding the functional polypeptide of interest to generate further extension sequences, wherein the extension sequences of step (d) act as primers and templates in one or more annealing and extending procedures, wherein recombined polynucleotide sequences are generated.