Patent Document ID: 20110235875
Application ID: 12732272
Patent Flag: 0

Claim One:
1. A method for optical segmentation of a biological sample positioned on a solid support and mounted on a fluorescent microscope comprising: transmitting light from a light source at a predetermined wavelength on to the biological sample wherein the light causes the biological sample to fluoresce; acquiring a fluorescent image of the biological sample using an image capture device; analyzing the fluorescent image utilizing, at least in part, featured based information or pixel intensity information to generate a masking pattern corresponding to a specific structure within the biological sample; transforming the masking pattern into a reformatted masking pattern to register the image of the image capture device with the spatial coordinates of a digital micro-mirror device (DMD); projecting the reformatted mask pattern onto the biological sample using the digital micro-mirror device (DMD) wherein said DMD is positioned between the light source and the biological sample and wherein the reformatted masked pattern is registered with the biological sample; acquiring a masked fluorescent image of the biological sample with the image capture device; and converting the masked fluorescent image into a digital image.