Patent Document ID: 9464122
Application ID: 13060143
Patent Flag: 1

Claim One:
1. A composition comprising a non-naturally occurring purified and/or isolated and stabilized tau oligomer and a buffer, wherein the tau oligomer comprises human recombinant tau protein produced recombinantly from bacteria in a buffer having a neutral pH, the tau oligomer being in a trimeric form having a first tau monomer containing a first cysteine, a second tau monomer containing a second cysteine, and a third tau monomer containing a third cysteine, wherein the first tau monomer is covalently attached to the second tau monomer and the second tau monomer is covalently attached to the third tau monomer by an intermolecular disulfide linkage between at least the first cysteine, the second cysteine and the third cysteine, and each monomer has an N-terminal and a C-terminal in a parallel orientation relative to each other in the trimeric form, and the tau oligomer is at least 90% purified, wherein the tau oligomer is formed without the addition of heparin or other polyanions.