Patent Document ID: 10047357
Application ID: 14481564
Patent Flag: 1

Claim One:
1. A method of evolution and expression of an antibody in a mammalian cell production host; the method comprising: a. evolving a template antibody to produce a set of mutant antibodies in a mammalian cell production host with antibody cell surface display, wherein the template antibody consists of an intact immunoglobulin molecule or a fragment of an immunoglobulin molecule and has a total of n amino acid residues, and said evolving comprises generating n-1 separate sets of mutant antibodies from the template antibody, each set comprising member antibodies having 19 different predetermined amino acid residues at a single predetermined position of the antibody, wherein each set of antibodies differs in the single predetermined position, and the number of different member antibodies generated is equivalent to [n−1]×19; b. screening the mutant antibodies for the at least one predetermined property, characteristic or activity; c. selecting an up-mutant antibody from the set of mutant antibodies based upon optimization of the at least one predetermined property, characteristic or activity compared to the template antibody; and d. expressing the up-mutant antibody in the same mammalian cell production host as was used in step (a).