Patent Document ID: 8771647
Application ID: 11766522
Patent Flag: 1

Claim One:
1. A method for producing a human photoaged skin model, comprising: irradiating a skin area of an immunodeficient mouse; and discontinuing irradiation when the skin area is in a condition suitable for use as a human photoaged skin model; wherein: the skin area comprises human skin transplanted to the immunodeficient mouse; irradiating the skin area comprises irradiating for a period of from six to eight consecutive weeks, during which: the skin area is irradiated with 40 to 50 mJ/cm 2 of UV-B radiation during a first week of the period; the skin area is irradiated with 50 to 100 mJ/cm 2 of UV-B radiation during a second week of the period; the skin area is irradiated with 60 to 100 mJ/cm 2 of UV-B radiation during a third week, a fourth week, a fifth week, and a sixth week of the period; and the skin area is optionally irradiated with 60 to 100 mJ/cm 2 of UV-B radiation during an optional seventh week and an optional eighth week of the period; irradiating the skin area comprises irradiating with a total dose of UV-B radiation of from approximately 1.65 J/cm 2 to approximately 2.85 J/cm 2 ; and the skin area is in the condition suitable for use as a human photoaged skin model when the skin area has at least one condition selected from the group consisting of wrinkles, sagginess, age spots, freckles, lowered elasticity, increased darkening, increased yellowing, and reduced keratinous layer water content.