Patent Document ID: 9528160
Application ID: 13834794
Patent Flag: 1

Claim One:
1. A method of selecting one or more patient-specific clonotypes correlated with a lymphoid neoplasm for monitoring a minimal residual disease thereof, the method comprising the steps of: (a) amplifying molecules of nucleic acid from T-cells and/or B-cells in a sample from a patient, the molecules of nucleic acid comprising recombined DNA sequences from T-cell receptor genes or immunoglobulin genes; (b) sequencing the amplified molecules of nucleic acid to form a clonotype profile containing the one or more patient-specific clonotypes; (c) comparing nucleotide sequences of the one or more patient-specific clonotypes from the clonotype profile to nucleotide sequences of clonotypes in a clonotype database, wherein the nucleotide sequences of clonotypes in a clonotype database are from at least one individual other than the patient to determine a presence, absence and/or level in the clonotype database of each of the nucleotide sequences of the one or more patient-specific clonotypes from the clonotype profile; and (d) selecting the one or more patient-specific clonotypes for monitoring the minimal residual disease which are each correlated with the lymphoid neoplasm and which are each absent from the clonotype database or at a level in the clonotype database that is below a predetermined frequency.