Patent Document ID: 20130045495
Application ID: 13660563
Patent Flag: 0

Claim One:
1. A method for selectively detecting vascular endothelial growth factor (VEGF) forms greater than 110 amino acids (VEGF 110+ ) in a biological sample, the forms including VEGF 121 , the method comprising the steps of: (a) contacting and incubating the biological sample with a capture reagent immobilized to a solid support, wherein the capture reagent comprises a monoclonal antibody that recognizes and binds specifically to residues greater than 110 of human VEGF; (b) separating the biological sample from the immobilized capture reagents; (c) contacting the immobilized capture reagent-target molecule complex with a detectable antibody that binds to the KDR and/or FLT1 receptor binding domains of VEGF or that binds to an epitope in VEGF 1-110; and (d) measuring the level of VEGF 110+ bound to the capture reagents using a detection means for the detectable antibody.