Patent Document ID: 8603749
Application ID: 12515262
Patent Flag: 1

Claim One:
1. A multiplex method for determining abundance profiles of one or more target polynucleotide sequences across a plurality of samples, comprising: amplifying and tagging target polynucleotides by PCR in each of said plurality of samples with an amplification primer comprising a high throughput sequencing adaptor, a sample-specific tag sequence of at least four nucleotides in length, and a priming sequence to amplify said target polynucleotide sequence(s); combining the amplified polynucleotides and sequencing the polynucleotide pool in high throughput, so as to determine the sequence of at least 300 tagged polynucleotides for each of said samples; assigning the nucleotide sequences to the originating samples by the nucleotide sequence of the sample-specific tag, thereby determining abundance profiles of the target polynucleotide sequence(s) across the samples.