Patent Document ID: 8481257
Application ID: 12158040
Patent Flag: 1

Claim One:
1. A high-throughput method for the discovery, detection and genotyping of a genetic marker in a plurality of samples, comprising the steps of: (a) providing DNA from a plurality of samples; (b) restricting the DNA with at least one restriction endonuclease to produce restriction fragments; (c) heating the restriction fragments to selectively dissociate the DNA fragments smaller than 120 bp; (d) for each sample, ligating adaptors to the restriction fragments 120 bp or larger to produce adaptor-ligated restriction fragments, wherein for each sample at least one adaptor is tagged with a different identifier tag; (e) amplifying the tagged adaptor-ligated restriction fragments with a primer pair, wherein at least one of the primers is complementary to at least part of the adaptor to produce a library of tagged amplified adaptor-ligated restriction fragments for each sample; (f) subjecting at least one library to high-throughput sequencing; (g) counting the number of sequences of each allele of a sequence polymorphism as a genetic marker in a library or between the libraries using the tag to distinguish between the libraries; and (h) determining a dominant or a co-dominant genotype of the genetic marker in the libraries based on the presence or absence of sequences of each allele of the sequence polymorphism, based on the high-throughput sequencing data obtained from step (f).