Patent Document ID: 20100255515
Application ID: 12817827
Patent Flag: 0

Claim One:
1. A method for detecting selective vascular endothelial growth factor (VEGF) forms (VEGF 110+ ) in a biological sample comprising the steps of: (a) contacting and incubating the biological sample with a capture reagent immobilized to a solid support, wherein the capture reagent is a antibody that recognizes same epitope as antibody 5C3 against human VEGF, said monoclonal antibody binding specifically to residues greater than 110 of human VEGF; (b) separating the biological sample from the immobilized capture reagents; (c) contacting the immobilized capture reagent-target molecule complex with a detectable antibody that binds to the KDR and/or FLT1 receptor binding domains of VEGF or that binds to an epitope in VEGF1-110; and (d) measuring the level of VEGF 110+ bound to the capture reagents using a detection means for the detectable antibody.