Patent Document ID: 9750826
Application ID: 14409668
Patent Flag: 1

Claim One:
1. A method for producing a glioblastoma mouse model comprising the steps of: (a) dividing a whole glioblastoma tissue, isolated from a patient, into 4 or more sections, collecting one or more pieces from each of the 4 or more sections, and homogenously mixing the collected pieces to prepare a homogenous mixture of the glioblastoma tissue pieces collected from each of the 4 or more sections of the whole glioblastoma tissue; (b) dissociating single cells of glioblastoma including glioblastoma stem cells from the homogenously mixed glioblastoma tissue pieces by sequentially performing mechanical dissociation and enzymatic dissociation with an enzyme mixture including protease and DNase; (b)′ removing erythrocytes by Percoll gradient centrifugation from a graft sample, which contains the glioblastoma cells as single cells obtained in step (b); and (c) orthotopically transplanting a graft sample comprising 1×10 5 to 2.0×10 5 glioblastoma cells obtained in step (b) in a volume of 5-10 μl, into the brain of an immunodeficient mouse by injecting the sample into the brain at the location of 1.5-2.5 mm left and 0.5-1.5 mm anterior to the bregma and 1.8-2.2 mm deep from the dura.