Patent Document ID: 9757402
Application ID: 14855645
Patent Flag: 1

Claim One:
1. A method for measuring cell-to-cell transmission of α-synuclein aggregates using a cell system comprising a first cell expressing a first fusion protein where an N-terminal fragment of a fluorescent protein and α-synuclein are fused; and a second cell expressing a second fusion protein where a C-terminal fragment of the fluorescent protein and α-synuclein are fused, the method comprising: co-expressing the first fusion protein and the second fusion protein in the first cell and the second cell, respectively, through co-culture of the first cell and the second cell; and quantitatively detecting, in the first cell and the second cell, a bimolecular fluorescence complementation (BiFC) fluorescent signal generated by linking the N-terminal fragment and the C-terminal fragment of the fluorescent protein, to analyze cell-to-cell transfer and co-aggregation of α-synuclein proteins derived from adjacent cells, wherein the cell model system includes a transgenic neuroblastoma cell line having Accession No. KCLRF-BP-00322 as the first cell and a transgenic neuroblastoma cell line having Accession No. KCLRF-BP-00323 as the second cell.