Patent Document ID: 7970548
Application ID: 10541791
Patent Flag: 1

Claim One:
1. A method for providing an integrated genetic and physical map of a genome or a part thereof, the method comprising the steps of: (a) providing at least two individual genetic markers for the genome or a part thereof in the form of a genetic map; (b) identifying at least one AFLP fragment characterizing each genetic marker by means of AFLP fingerprinting, employing at least one forward AFLP primer and at least one reverse AFLP primer; (c) providing a library of clones comprising fragments of the genome or a part thereof, which is an artificial chromosome library; (d) pooling individual clones in the library to generate a multitude of pools, each pool containing a multitude of individual clones from the library; (e) generating an AFLP fingerprint for each of the pools employing forward AFLP primers and reverse AFLP primers; (f) identifying in the multitude of pools a pool in which an AFLP fragment that was identified in step (b) is present in the fingerprint of the pool; (g) generating an AFLP fingerprint for each of the individual clones in the pool identified in step (f) employing forward AFLP primers and reverse AFLP primers, and identifying the clone containing the AFLP fragment identified in step (b) in such clone's AFLP fingerprint; (h) aligning the individual clone identified in step (g) to generate a contig; (i) repeating steps (f)-(h) for at least a second AFLP fragment identified in step (b) whereby the second, or a further, AFLP fragment characterizes a second, or a further, genetic marker; and, (j) linking at least two contigs obtained in step (h); thereby obtaining said integrated genetic and physical map of the genome or a part thereof, which comprises at least two genetic markers; wherein: (1) the forward AFLP primers used in steps (b) and (e) comprise K selective nucleotides at the 3′-end, (2) the reverse AFLP primers used in steps (b) and (e) comprise L selective nucleotides at the 3′-end, (3) the forward AFLP primers used in step (g) comprise M selective nucleotides at the 3′-end, and (4) the reverse AFLP primers used in step (g) comprise N selective nucleotides at the 3′ end, and wherein K, L, M, N are integers with a value from 0 to 10, and wherein the forward and reverse AFLP primers used in steps (b) and (e) are of higher selectivity, and the forward and reverse AFLP primers used in step (g) are of lower selectivity.