Patent Document ID: 20150307929
Application ID: 14795774
Patent Flag: 0

Claim One:
1. A method of generating an amplicon library from genomic DNA comprising: a) contacting a portion of a genomic DNA with a plurality of different target-specific primers having a cleavable group, and a polymerase under amplification conditions and producing a plurality of different target-specific amplicons by extending one or more of the plurality of target-specific primers having a cleavable group; b) digesting at least one of the plurality of different target-specific amplicons with a cleaving reagent; and c) ligating an adapter to the end of the at least one digested different target-specific amplicon and producing at least one adapter-ligated target-specific amplicon, wherein the adapter is not complementary to 20 contiguous nucleotides from a 3′ end or 5′ end of the at least one digested different target-specific amplicon.