Patent Document ID: 8163700
Application ID: 12945683
Patent Flag: 1

Claim One:
1. A method of decreasing the inhibition of axonal outgrowth by a neuron, comprising contacting the neuron with an effective amount of a composition comprising a polypeptide selected from the group consisting of: (i) a first polypeptide comprising a neuronal binding region consisting of an Ig-like domain 1 (Igd1) of a protein, said first protein having at least 75% sequence identity to SEQ ID NO: 2, 4 or 6, said first polypeptide further comprising at least one of MAG Ig-like domain 5 (Igd5) amino acid residues 432 to 488 and lacking MAG Igd5 amino acid residues 450 to 490 or 450 to 453 of SEQ ID NO: 2, 4, or 6, wherein the ability of said first polypeptide to regulate neurite outgrowth as compared to endogenous or soluble MAG is reduced or eliminated without eliminating binding of said first polypeptide to neuronal surfaces; (ii) a second polypeptide comprising a MAG Ig5 amino acid sequence selected from the group consisting of amino acid residues 433-457, 422-451, 442-471, 446-455, 450-455, 466-471, and 472-477 of SEQ ID NO:2, 4, or 6, wherein the ability of said second polypeptide to regulate neurite outgrowth as compared to endogenous or soluble MAG is reduced or eliminated without eliminating binding of said second polypeptide to neuronal surfaces; (iii) a third polypeptide that differs from wild-type MAG in that said third polypeptide lacks residues 466-478 said residues being numbered according to an of SEQ ID NO: 2, 4, or 6, wherein the ability of said third polypeptide to regulate neurite outgrowth as compared to endogenous or soluble MAG is reduced or eliminated without eliminating binding of said third polypeptide to neuronal surfaces; and (iv) a fourth polypeptide consisting of amino acid residues 437-441 or 462-486 of SEQ ID NO: 2, 4 or 6.