Patent Document ID: 20080003661
Application ID: 11743668
Patent Flag: 0

Claim One:
1. A method for developing a culture medium using in silico analysis and genome information, comprising the steps of: (a) constructing a metabolic network using genome information of target eukaryotic cell or prokaryotic cell; (b) removing any one among external metabolites from the constructed metabolic network and conducting metabolic flux analysis using in silico simulation; (c) determining the removed external metabolite as a component of a minimal synthetic medium when a objective function value is 0 after the in silico simulation, wherein the objective function value is 0 if any one of the components required by eukaryotic or prokaryotic cell cannot be produced, or returning to step (b) when the objective function value is not 0; (d) confirming whether a relevant metabolite can be produced internally and used without providing it externally when relevant metabolic reaction formula exists after examining the relevant reaction formula and deficient enzymes through comparison with other organism species synthesizing the metabolite by itself; (e) determining components of a minimal synthetic medium determined by repeating steps (b) to (d) as a minimal synthetic medium for in silico; and (f) determining a final culture medium after culturing target eukaryotic or prokaryotic cells in the determined minimal synthetic medium for in silico.