Patent Document ID: 20020065609
Application ID: 09829855
Patent Flag: 0

Claim One:
1. A method to determine the genetic diversity of a population comprising the steps: a) providing a sample of interest containing a population of interest; b) isolating genomic DNA from the sample; c) performing PCR on the genomic DNA to amplify a subregion comprising a polymorphic site of interest using an upstream primer comprising a binding site for a solid support and a downstream primer to produce an amplified fragment; d) cleaving the amplified fragment with a restriction enzyme that cleaves the amplified fragment at a site close to the polymorphic region; e) binding the cleaved amplified fragment to the solid support to form an immobilized product; f) splitting the immobilized product into two pools; g) attaching a double-stranded linker to the immobilized product of each pool, wherein the linker comprises a Type IIS restriction enzyme site; h) digesting the product with the Type IIS restriction enzyme to form a tag; i) ligating the tags to one another to form ditags; j) amplifying the ditags by PCR; k) cleaving the ditags with the restriction enzyme of step c) and ligating the ditags to form a concatemer; l) cloning the concatemer into a vector; and m) determining the sequence the concatemer.