Patent Document ID: 10106850
Application ID: 15683252
Patent Flag: 1

Claim One:
1. A method for detection of genetic variation in two or more members of a population, comprising: amplifying nucleic acid fragments of at least a first and second member of a population to obtain amplified nucleic acid fragments, wherein the amplified nucleic acid fragments of the first member of the population comprises sequences from a nucleic acid sample of the first member of the population and a first unique identifier sequence, wherein the amplified nucleic acid fragments of the second member of the population comprises sequences from a nucleic acid of the second member of the population and a second unique identifier sequence, and wherein the first and second unique identifier sequences indicate different sample origins of the nucleic acid fragments; sequencing a pool of the amplified nucleic acid fragments using high throughput sequencing technology; clustering the sequences of amplified nucleic acid fragments of the first and second members of the population and comparing the clustered sequences to a reference sequence to identify genetic variation; and identifying the first and second member of the population as carrying the genetic variation using the first and second unique identifier sequences.