Patent Document ID: 20060057616
Application ID: 11204854
Patent Flag: 0

Claim One:
1. A method for amplifying genetic sequences, comprising the steps of: (1) first, reverse transcribing RNA to DNA, and conducting PCR using at least one outer primer having a sequence within the class: SEQ ID NO: 1 (AAYGGGTGAGTAACACGT) for Gram positive bacteria or, SEQ ID NO: 8 (RAYGGGTGAGTAAYGYMT) for Gram negative bacteria, and at least one primer having a sequence within the class: SEQ ID NO: 5 (CCCGRGAACGTATTCACSG), (2) second, conducting nested PCR, using inner primers, comprising at least one primer having a sequence within the class: SEQ ID NO: 6 (CTACGGGAGGCWGCAGTRRGGAAT), and at least one primer having a sequence within the class: SEQ ID NO: 7 (WGGGTATCTAATCCTRTTTGMTCCCCW), wherein R = G or A, S = G or C, W = A or T, M = A or C, and Y = T or C.