Patent Document ID: 20120190005
Application ID: 13321877
Patent Flag: 0

Claim One:
1. A method for a rational cell culturing process comprising: a) providing a self-replicating organism, b) culturing said organism according to a process A, c) culturing said organism according to a process B, which is different from process A, d) employing a comparative method suitable to distinguish between the different performance levels of said cell in process A versus process B, e) identifying biological functions and metabolic pathways which relate to the two different processes A and B and their respective performance levels, f) selecting components of deregulated metabolic pathways according to the following criteria: i. signal intensity values≧40, preferably ≧80 ii. p-value≦0.1, preferably ≦0.05 iii. log2 fold change (FC) of |FC|≧1.5, preferably ≧2.0, iv. FC criteria (iii) to be satisfied for at least one time point of the cultivation time, preferably for cultivation day 8, g) identifying at least one deregulated component of the lipid metabolism, h) cultivating said organism of step a) according to a process C, which is different from process A and process B, whereby said cultivation process C is adjusted by adding, modifying or omitting at least one component of step g).