Patent Document ID: 10082499
Application ID: 14712357
Patent Flag: 1

Claim One:
1. A method for preparation of labeled metabolic products, comprising the steps: (a) providing a biological sample in vitro; (b) contacting the biological sample with a labeling buffer comprising a labeled substrate, wherein the substrate comprises at least one carbon atom and represents an educt or intermediate of a metabolic process of the biological sample and wherein the label is a stable isotope; (c) washing the biological sample using a wash-buffer, wherein said wash-buffer comprises a carbon resource being a relevant nutrient resource of the biological sample such that the biological sample is precluded from carbon deficiency during said washing, wherein said carbon resource comprises the substrate according to step b), wherein said substrate in the wash-buffer may be labeled or unlabeled; (d) quenching the biological sample such that metabolic processes within the biological sample are slowed down or stopped; and (e) extracting the labeled metabolic product from the biological sample.