Patent Document ID: 8642263
Application ID: 11719033
Patent Flag: 1

Claim One:
1. A high-throughput method for quantitative measurement of global genomic 5-methylcytosine content, comprising: obtaining a test sample comprising genomic DNA from a human tissue sample; and determining, using a quantitative real-time, bisulfite-based PCR assay, the methylation status of at least one CpG dinucleotide sequence of at least two repetitive genomic sequences selected from the group consisting of Alu, LINE-1, and Chr1 centromeric Satα and juxtacentromeric Sat2 repeat sequences, wherein the methylation status of at least one CpG dinucleotide sequence of the Alu or LINE-1 repeat sequence is determined, and wherein the determined methylation status of the at least two repetitive genomic sequence significantly correlates with global genomic 5-methylcytosine content, and wherein a quantitative measurement of global 5-methylcytosine content is thereby provided.