Patent Document ID: 9458509
Application ID: 13811126
Patent Flag: 1

Claim One:
1. A high-input detector module for classifying a cell status based on detecting whether an input microRNA is expressed at a specific level or higher than a reference level, said high-input detector module comprising: (a) an inducible promoter sequence operably linked to: (i) a repressor sequence encoding a repressor product, and (ii) one or more target sequences for microRNA, said one or more target sequences for microRNA are the target sequences of the one or more input microRNAs the module is designed to detect; (b) a repressible promoter sequence operably linked to an output sequence encoding an output product, said repressor product being specific for the repressible promoter sequence; and (c) one or more regulatory units, comprising a constitutive or inducible promoter sequence operably linked to: (i) a sequence encoding a transcriptional activator product, said transcriptional activator product activates the inducible promoter sequence operably linked to the repressor sequence and the one or more target sequences for microRNA of the module, and (ii) one or more target sequences for microRNA, said one or more target sequences for microRNA are the target sequences of the one or more input microRNAs the module is designed to detect.