Patent Document ID: 9090874
Application ID: 13127142
Patent Flag: 1

Claim One:
1. A method for producing a recombinant dopaminergic neuron, the method comprising: (a) providing adult human olfactory epithelial-derived stem cells; (b) introducing, into the adult human olfactory epithelial-derived stem cells, one or more transgenes encoding a nurr-1 polypeptide, a pitx3 polypeptide and a Imx1a polypeptide, to produce transgenic adult human olfactory epithelial-derived stem cells; and (c) culturing the transgenic adult human olfactory epithelial-derived stem cells to produce recombinant dopaminergic neurons, wherein the recombinant dopaminergic neurons produce increased levels of intracellular dopamine relative to the levels of intracellular dopamine produced by adult human olfactory epithelial-derived stem cells expressing the nurr-1 polypeptide, the pitx3 polypeptide or the Imx1a polypeptide.