Patent Document ID: 9388452
Application ID: 13638919
Patent Flag: 1

Claim One:
1. A method for modeling protein stability over time, comprising the steps: (a) determining a stability of a protein at a plurality of different temperatures within a specific temperature range, wherein the protein stability comprises a measure of retention of enzymatic activity over time; (b) creating an Arrhenius plot comprising the stability of the protein at the plurality of temperatures determined in step (a), the Arrhenius plot having a linear portion at lower temperatures and a non-linear portion at higher temperatures, the linear and non-linear portions joining at an inflection point on the Arrhenius plot; (c) identifying the inflection point at the junction between the linear portion and the non-linear portion of the Arrhenius plot created in step (b); (d) determining an assay temperature consisting of the temperature at the inflection point of the Arrhenius plot created in step (b); (e) performing an accelerated stability study of the protein at the assay temperature determined in step (d), wherein the stability study comprises determining a retention of enzymatic activity over time; and (f) determining a stability of the protein at a second temperature based on the accelerated stability data obtained in step (e), wherein the second temperature is lower than the assay temperature.