Patent Document ID: 7771921
Application ID: 11536576
Patent Flag: 1

Claim One:
1. A process for preparing sperm cell samples, the process comprising: collecting semen from a male mammal; freezing said semen to form frozen semen; thawing said frozen semen to form frozen-thawed semen; incubating sperm cells contained within said frozen-thawed semen in a concentration of Hoechst 33342 stain of greater than 40 micro-molar; establishing the temperature at which said sperm cells are incubated between about 30 degrees centigrade and about 40 degrees centigrade; adjusting a duration of time said sperm cells are incubated in said concentration of Hoechst 33342 stain; staining said sperm cells with sufficient uniformity to allow X-chromosome bearing sperm cells to be differentiated from Y-chromosome bearing sperm cells based upon the magnitude of fluorescence; determining the sex characteristic of a plurality of sperm cells contained within said frozen-thawed semen; separating said sperm cells according to the determination of their sex characteristic; isolating sperm cells separated according to the determination of their sex in a collection element; establishing a sample from said sperm cells isolated in said collection element; wherein fertilizing at least one egg of a female mammal with said sample at success levels of at least about 70% of success with spermatozoa that have not been separated and/or frozen is achieved.