Patent Document ID: 10041932
Application ID: 14866791
Patent Flag: 1

Claim One:
1. A method comprising steps of: (i) obtaining a first set of blood samples from human subjects having autism spectrum disorder (ASD) and a second set of blood samples from human subjects having non-ASD developmental delay (DD); (ii) measuring levels of metabolites in the first and second set of samples, wherein the metabolites comprise xanthine, gamma-CEHC, hydroxy-chlorothalonil, 5-hydroxyindoleacetate (5-HIAA), indoleacetate, p-cresol sulfate, 1,5-anhydroglucitol (1,5-AG), 3-(3-hydroxyphenyl)propionate, 3-carboxy-4-methyl-5-propyl-2-furanpropanoate (CMPF), 3-indoxyl sulfate, 4-ethylphenyl sulfate, 8-hydroxyoctanoate, hydroxyisovaleroylcarnitine (C5), isovalerylglycine, lactate, N1-Methyl-2-pyridone-5-carboxamide, pantothenate (Vitamin B5), phenylacetylglutamine, pipecolate, 3-hydroxyhippurate, and octenoylcarnitine; (iii) determining a distribution profile for the metabolites in the first set and second set of samples, wherein the distribution profile has a lower end and an upper end; (iv) defining for each metabolite: (a) a first threshold at the lower end of the distribution profile (left tail), wherein the first threshold is the 15 th percentile; and (b) a second threshold at the upper end of the distribution profile (right tail), wherein the second threshold is the 90 th percentile.