Patent Document ID: 9828634
Application ID: 15004188
Patent Flag: 1

Claim One:
1. A method of identifying differentiation markers expressed in pancreatic β-cell lineage cells, comprising: (a) culturing an isolated primary cell under conditions that comprise exogenously expressing Oct4, Sox2, cMyc, and Klf4 in said primary cell, such that said primary cell de-differentiates to generate an induced pluripotent stem cell (iPSC); (b) treating said iPSC under conditions such that said iPSC differentiates into a pancreatic β-cell, wherein said treating comprises: (i) culturing the iPSC in a chemically defined medium comprising fibroblast growth factor, Activin A and bone morphogenetic protein, (ii) culturing the cells produced from step (i) in the presence of chemically defined medium comprising insulin, transferrin, selenium, a fibroblast growth factor and nicotinamide, (iii) culturing the cells produced from step (ii) in the presence of a chemically defined medium comprising insulin, transferrin, selenium, retinoic acid, a bone morphogenetic protein inhibitor and nicotinamide; and (iv) culturing the cells produced from step (iii) in the presence of a serum-free medium comprising an insulin-like growth factor, insulin, nicotinamide, exendin-4 and/or GLP-1, a TGF-β inhibitor, and an agent that increases cAMP; and (c) identifying molecular markers expressed during differentiation, wherein said markers are indicative of differentiation of said iPS cell into a pancreatic β-cell.