Patent Document ID: 7853410
Application ID: 11975638
Patent Flag: 1

Claim One:
1. A method of making a recombinant nucleic acid, the method comprising: providing a plurality of parental character strings corresponding to a plurality of nucleic acids, which character strings, when aligned for maximum identity, comprise at least one region of heterology; aligning the character strings; defining a set of character string subsequences, which set of subsequences comprises subsequences of at least two of the plurality of parental character strings; providing a set of oligonucleotides corresponding to the set of character string subsequences; annealing members of the set of oligonucleotides with one another in vitro; after annealing, elongating one or more members of the set of oligonucleotides with a polymerase, or ligating at least two members of the set of oligonucleotides with a ligase, thereby producing one or more recombinant nucleic acid; selecting the recombinant nucleic acid, or a polypeptide encoded by the recombinant nucleic acid for a desired trait or property, thereby providing a selected recombinant nucleic acid; and recombining the selected recombinant nucleic acid with one or more of (i) a nucleic acid homologous to a parental character string, and (ii) an oligonucleotide member from the set of oligonucleotides.