Patent Document ID: 10108778
Application ID: 14291930
Patent Flag: 1

Claim One:
1. A system for real-time identification of biological material in a sample potentially containing a plurality of nucleic acid molecules each from a corresponding one of a plurality of organisms, the sample being sequenced and analyzed in real-time, the system comprising: a computer system comprising one or more processors programmed with computer program instructions that, when executed, cause the computer system to: cause at least first nucleotide sequence information to be generated, the first nucleotide sequence information comprising a first string of one or more nucleotides generated from a first one of the plurality of nucleic acid molecules at a first time during real-time sequencing; compare the first string of one or more nucleotides and one or more reference nucleic acid sequences stored in a reference database; determine a probabilistic match between the first string of one or more nucleotides and the one or more reference nucleic acid sequences; determine that the probabilistic match does not satisfy a predetermined threshold; responsive to the determination that the probabilistic match does not satisfy the predetermined threshold, cause at least second nucleotide sequence information to be generated, the second nucleotide sequence information comprising a second string of one or more nucleotides generated from the first one of the plurality of nucleic acid molecules at a second time after the first time during real-time sequencing, wherein the second string of nucleotides includes at least one additional nucleotide generated from the first one of the plurality of nucleic acid molecules after the first time; compare the second string of one or more nucleotides and the one or more reference nucleic acid sequences stored in the reference database; determine a second probabilistic match between the second string of one or more nucleotides and at least one of the one or more reference nucleic acid sequences; determine that the second probabilistic match satisfies the predetermined threshold; determine a likelihood that one or more organisms corresponding to the at least one of the one or more reference nucleic acid sequences are present in the sample based at least in part on the second probabilistic match.