Patent Document ID: 10130683
Application ID: 14823637
Patent Flag: 1

Claim One:
1. A method of treating traumatic neuronal injury, comprising: providing human umbilical cord blood; centrifuging the human umbilical cord blood to obtain pelleted cord blood; isolating a mononuclear cell layer from the pelleted cord blood to provide human umbilical cord blood derived mononuclear cells; determining CD34 expression in the human umbilical cord blood derived mononuclear cells for use in a composition wherein the human umbilical cord derived mononuclear cells expressing less than 4% CD34+ cells are used in a composition; administering a therapeutically effective amount of the composition comprising the human umbilical cord blood derived mononuclear cells expressing less than 4% CD34+ cells and granulocyte colony stimulating factor to a patient suffering from the traumatic neuronal injury; wherein the human umbilical cord blood derived mononuclear cells are administered at a dosage of about 9.41×10 6 cells/kg to about 1.78×10 7 cells/kg and the granulocyte colony stimulating factor is administered at a dosage of about 300 mg/kg; wherein motor function is improved up to at least 56 days post traumatic neuronal injury.