Patent Document ID: 9677070
Application ID: 13802255
Patent Flag: 1

Claim One:
1. A recombinant polynucleotide vector for integrating a target gene into a chicken immunoglobulin gene heavy chain locus, comprising: (a) a chicken immunoglobulin V H gene upstream nucleic acid sequence region comprising a sequence homologous to the 5′ side of the start codon of endogenous genomic DNA encoding a chicken immunoglobulin V H gene; (b) a target gene that comprises a rearranged chicken immunoglobulin V H -D-J H gene that has been isolated from a V H library of already rearranged V H -D-J H regions isolated from a population of chicken bursa of Fabricius cells, wherein said V H -D-J H gene is rearranged such that the V H , D and J H genes are joined together; and (c) a chicken immunoglobulin J H gene downstream nucleic acid sequence region comprising a sequence homologous to the 3′ side of the splice site of endogenous genomic DNA encoding a chicken immunoglobulin J H gene; wherein the target gene, upon being integrated into the chicken immunoglobulin heavy chain locus of a DT40 cell, is capable of undergoing either or both of (i) somatic hypermutation in an immunoglobulin V H region-encoding sequence, and (ii) gene conversion between a rearranged chicken immunoglobulin V H -encoding nucleic acid sequence and a DT40 V H pseudogene nucleic acid sequence.