Patent Document ID: 9122777
Application ID: 12816232
Patent Flag: 1

Claim One:
1. A method for determining coronary artery disease risk in a subject, comprising: performing a reverse transcriptase polymerase chain reaction (RT-PCR) assay on a sample from the subject by using a plurality of distinct primer and probe sets that specifically hybridize to mRNA corresponding to each gene in at least one term selected from the group consisting of term 1, term 2, term 3, term 4, term 5, term 6, and term 7; wherein term 1 comprises gene 1, gene 2, and gene 3, wherein gene 1 is AF161365, wherein gene 2 is HNRPF or ACBD5, and wherein gene 3 is TFCP2 or DDX18; wherein term 2 comprises gene 4, gene 5, and gene 6, wherein gene 4 is AF289562 or CD248, wherein gene 5 is HNRPF or ACBD5, and wherein gene 6 is TFCP2 or DDX18; wherein term 3 comprises gene 7, gene 8, gene 9, and gene 10 wherein gene 7 is CD79B or CD19, wherein gene 8 is SPIB or BLK, wherein gene 9 is CD3D or LCK, and wherein gene 10 is TMC8 or CCT2; wherein term 4 comprises gene 11, gene 12, gene 13, and gene 14, wherein gene 11 is S100A12 or MMP9, wherein gene 12 is CLEC4E or ALOX5AP, wherein gene 13 is S100A8 or NAMPT, and wherein gene 14 is RPL28 or SSRP1; wherein term 5 comprises gene 15, gene 16, gene 17, gene 18, and gene 19, wherein gene 15 is S100A12 or MMP9, wherein gene 16 is CLEC4E or ALOX5AP, wherein gene 17 is S100A8 or NAMPT, wherein gene 18 is AQP9 or GLT1D1, and wherein gene 19 is NCF4 or NCF2; wherein term 6 comprises gene 20, gene 21, gene 22, gene 23, gene 24, gene 25, and gene 26, wherein gene 20 is CASP5 or H3F3B, wherein gene 21 is IL18RAP or TXN, wherein gene 22 is TNFAIP6 or PLAUR, wherein gene 23 is IL8RB or BCL2A1, wherein gene 24 is TNFRSF10C or PTAFR, wherein gene 25 is KCNE3 or LAMP2, and wherein gene 26 is TLR4 or TYROBP; and wherein term 7 comprises gene 27, gene 28, gene 29, and gene 30, wherein gene 27 is SLAMF7 or CX3CR1, wherein gene 28 is KLRC4 or CD8A, wherein gene 29 is CD3D or LCK, and wherein gene 30 is TMC8 or CCT2; generating, based on the assay, a dataset comprising data representing mRNA expression levels corresponding to each of the genes; obtaining data representing age of the subject and data representing gender of the subject; and generating, by a computer processor, a score indicative of coronary artery disease (CAD) risk by mathematically combining the data representing the mRNA expression levels, the data representing the age of the subject, and the data representing the gender of the subject, wherein a higher score relative to a control subject having less than 50% stenosis in all major vessels indicates an increased likelihood that the subject has CAD or a lower score relative to a control subject having greater than or equal to 50% stenosis in at least one major coronary vessel indicates a decreased likelihood that the subject has CAD.