Patent Document ID: 8969043
Application ID: 13321877
Patent Flag: 1

Claim One:
1. A method for producing a product of interest comprising a) providing a mammalian host cell, b) culturing said cell according to a process A, c) culturing said cell according to a process B, which is different from process A, d) employing a comparative method suitable to distinguish between the different performance levels of said cell in process A versus process B, e) identifying biological functions and pathways which relate to the two different processes A and B and their respective performance levels, f) selecting genes of deregulated metabolic pathways according to the following criteria: i. signal intensity values ≧40, ii. p-value ≦0.1, iii. log 2 fold change (FC) of |FC|≧1.5, iv. FC criteria (iii) to be satisfied for at least one time point of the cultivation time, preferably for cultivation day ≦8, g) identifying at least one deregulated gene of lipid metabolism, h) cultivating said cell of step a) according to a process C, which is different from process A and process B, whereby said cultivation process C is adjusted by adding, modifying or omitting at least one component metabolized by an enzyme encoded by the identified gene of step g).