Patent Document ID: 9453262
Application ID: 14066967
Patent Flag: 1

Claim One:
1. A kit comprising at least five pairs of tagged forward and reverse primer pairs, and a software program: wherein the primer pairs are disposed separately, and each forward and reverse primer comprises, in 5′ to 3′ order: a priming sequence, a tag sequence of from 4 to 36 nucleotides in length, and a probe sequence targeting a variable genetic region for amplification, wherein: (A) the priming sequence is the same between said primer pairs, with the proviso that forward and reverse primers may have the same or different priming sequences; (B) the tag sequence in each forward and reverse primer pair is the same, and different from the tag sequence of the other primer pairs; and (C) the probe sequences between the primer pairs are the same, and target for amplification a variable genetic region selected from: a 16S rRNA sequence, an 18S rRNA sequence, and an ITS sequence; and wherein the software program deconvolves, from a mixture of nucleotide sequences, the profile of nucleotide sequences for a plurality of samples by identifying said tags in the sequences.