Patent Document ID: 20020114855
Application ID: 09789528
Patent Flag: 0

Claim One:
1. A method for the detection of the analeptic and psychostimulant properties of extract obtained from Acorus calamus, using Drosophila melanogaster Sh 5 eag 1 said method comprising the steps of: (i) preparing acetone and methanol extracts of leaves of the plant Acorus calamus, (j) treating Sh 5 eag 1 Drosophila melanogaster mutant flies in the media selected from normal fly food, normal food mixed with phenobarbital, normal food mixed with ethanol, normal food mixed with the alcoholic sample prepared above, normal food mixed with both the alcoholic sample prepared above and phenobarbital and normal food mixed with both the alcoholic sample of step (a) and ethanol, (k) subjecting differently treated flies to ether anesthesia and then determining extent of time required by them to recover, (l) observing spontaneous locomotor activity in the differently treated flies and increase in the spontaneous locomotor activity is indicative of analeptic activity of extract of Acorus calamus against ethanol, (m) Observing the recovery time following ether and chloroform anesthesia in flies not pretreated with phenobarbital wherein quick recovery of the flies is indicative of analeptic activity of the extract against phenobarbital, (n) Observing the recovery time in flies anesthetized with ether and pretreated with phenobarbital, wherein quick recovery of the flies is indicative of analeptic activity of the extract, (o) observing the spontaneous locomotor activity of the flies under the influence of ethanol, wherein quick recovery of the flies is indicative of analeptic activity of the extract (p) observing the spontaneous locomotor activity of the flies not under the influence of ethanol, wherein quick recovery of the flies is indicative of the psychostimulant activity of the extract.