Patent Document ID: 9029163
Application ID: 13214357
Patent Flag: 1

Claim One:
1. A method of analyzing the effect of a parameter on the stability of a protein, the method comprising: providing a first solution with a first value of said parameter and a predetermined minimum concentration of a chemical denaturant, a second solution having said first value of said parameter and a predetermined maximum concentration of said chemical denaturant, a third solution having a second value of said parameter and said predetermined minimum concentration of said chemical denaturant, and a fourth solution having said second value of said parameter and said predetermined maximum concentration of said chemical denaturant; combining, by an apparatus, said first solution and said third solution in various ratios to create a plurality of fifth solutions, each solution of said plurality of fifth solutions having a different value of said parameter between said first value and said second value, and having said predetermined minimum concentration of said chemical denaturant; combining, by said apparatus, said second solution and said fourth solution in various ratios to create a plurality of sixth solutions, each solution of said plurality of sixth solutions having a different value of said parameter between said first value and said second value such that each solution of said plurality of sixth solutions corresponds to one solution of said plurality of fifth solutions, and has said predetermined maximum concentration of said chemical denaturant; selecting, by said apparatus, a solution of said plurality of fifth solutions and a corresponding solution of said plurality of sixth solutions, such that said selected fifth and sixth solutions have the same value of said parameter; mixing, by said apparatus, said selected fifth and corresponding selected sixth solutions in various ratios to create a plurality of seventh solutions, each solution of said plurality of seventh solutions having the same value for said parameter and a concentration of chemical denaturant between said predetermined minimum concentration and said predetermined maximum concentration; measuring an observable property of each solution of said plurality of seventh solutions; and creating a denaturation graph based on said measured observable property of each solution of said plurality of seventh solutions and said concentration of said chemical denaturant of each solution of said plurality of seventh solutions.