Patent Document ID: 20130261012
Application ID: 13802255
Patent Flag: 0

Claim One:
1. A recombinant polynucleotide vector for integrating a target gene into a chicken immunoglobulin gene heavy chain locus, comprising: (a) a chicken immunoglobulin V H gene upstream nucleic acid sequence region; (b) a target gene that comprises a rearranged chicken immunoglobulin V H -D-J H gene that has been isolated from a population of chicken bursa of Fabricius cells; and (c) a chicken immunoglobulin J H gene downstream nucleic acid sequence region, wherein the target gene, upon being integrated into the chicken immunoglobulin heavy chain locus of a DT40 cell, is capable of undergoing either or both of (i) somatic hypermutation in an immunoglobulin V H region-encoding sequence, and (ii) gene conversion between a rearranged chicken immunoglobulin V H -encoding nucleic acid sequence and a DT40 V H pseudogene nucleic acid sequence.