Patent Document ID: 20120006278
Application ID: 13257746
Patent Flag: 0

Claim One:
1. A method for breeding scallop population with orange-red adductor muscles, wherein the method comprises: (1) randomly collecting adult scallops from wild scallop populations; slightly opening shells of the adult scallops, observing colors of adductor muscles in the shells; selecting and growing scallops that have orange-red adductor muscles; separating male and female scallops; promoting selected scallops to sexual maturity; (2) using high performance liquid chromatography (HPLC) technique to detect carotenoid contents in the orange-red adductor muscles of the selected scallops from (1); selecting male and female scallops with above 15 μg/g carotenoid contents as parental seeds; (3) repeating the steps of (1) and (2) for four generations until 100% of breeding scallops have stable carotenoid contents and an average carotenoid content of two year old scallops is above 30 μg; (4) then collecting scallops with orange-red adductor muscles from cultivated scallop populations or wild scallop populations; opening shells of the scallops and observing appearance of sex glands; selecting scallops with well developed sex glands as breeding parents; (5) putting the selected scallops of (4) in indoor culturing pools with filtered sea water; washing shells of the parental seeds to remove additive mud and other materials; gradually and slowly increasing culturing temperature with less than 1° C. increase every day until culturing temperature is 8-8.5° C. higher than environmental sea water temperature; culturing the scallops for additional 20 days to induce sexual maturity; (6) separating males and females of sexually matured breeding parents from (5); drying the scallop in dark for 30-40 minutes; inducing the breeding parents to spawn eggs and sperms using warmer filter sea water which is 2° C. higher than the culturing sea water temperature of (5); separately collecting the eggs and sperms; (7) mixing the eggs with the sperms; observing fertilized eggs under a microscope, each egg being surrounded by 3-4 sperms; and fertilization being finished within 1.5 hour; and (8) incubating and growing the fertilized eggs of (7) according to normal cultivation methods.