Patent Document ID: 20030165923
Application ID: 10236363
Patent Flag: 0

Claim One:
1. A method for identifying genetic markers comprising: a) obtaining genomic DNA from a first source having a first phenotype or trait of interest; b) digesting said DNA with at least one restriction endonuclease having a degenerate recognition or cleavage sequence comprising at least one degenerate base said degenerate sequence represented by the formula N m, where N is the extent of degeneracy, and m is the number of degenerate bases, to produce restriction fragments having N m different single-stranded overhangs for each restriction endonuclease; c) ligating restriction fragments having identical overhangs with a series of adapters whose sequences are complementary to said overhangs; d) amplifying said restriction fragments; e) separating said amplified restriction fragments to produce a restriction fragment pattern; f) obtaining genomic DNA from at least one additional source having a second phenotype or trait of interest; g) repeating b) through e) for said DNA from said at least one additional source; h) comparing the restriction fragment patterns from each source; i) selecting restriction fragments associated with a phenotype or trait of interest; and j) using said selected restriction fragments as markers for said phenotype or trait of interest.