Patent Document ID: 10081839
Application ID: 15413200
Patent Flag: 1

Claim One:
1. A method for measuring the amounts of fetal chromosome segments in a maternal blood sample, comprising: obtaining fetal and maternal chromosome segments from cell-free DNA of a maternal blood sample; ligating at least one adapter to the chromosome segments, wherein the at least one adapter comprises a universal amplification sequence; performing universal amplification using universal primers that bind to the universal amplification sequence to generate amplified chromosome segments; performing clonal amplification of the amplified chromosome segments to generate clonally amplified chromosome segments; measuring the amounts of clonally amplified fetal chromosome segments and the amounts of clonally amplified maternal chromosome segments by performing next generation sequencing, wherein the measuring is performed without prior knowledge of fetal genotype and comprises quantitative allele measurements performed irrespective of allele value; and comparing the measured amounts of clonally amplified maternal chromosome segments with the measured amounts of clonally amplified fetal chromosome segments.