Patent Document ID: 9489474
Application ID: 13576442
Patent Flag: 1

Claim One:
1. A method of identifying a point of action of a stimulus applied to an electrically active cell, the method comprising: generating action potentials and associated cellular parameters using a model comprising a first submodel based on Hodgkin-Huxley calculations, a second submodel operably linked thereto and based on reaction kinetics of cell metabolism, and a third submodel operably linked to the first and second submodels and based on Boolean dynamics representing signaling and associated cellular processes; storing a library of the generated action potentials and associated cellular parameters; applying the stimulus to the electrically active cell in vitro so that the electrically active cell generates an action potential; and comparing the cell-generated action potential with the generated action potentials stored in the library, wherein a match is predictive of the point of action of the stimulus according to cellular parameters stored in the library for the matching generated action potential, wherein the first, second, and third submodels are operably linked such that the model quantifies changes in intracellular processes based on physiological changes responsive to reaction kinetics of cell metabolism and signaling and associated cellular processes.