Patent Document ID: 9290811
Application ID: 14735066
Patent Flag: 1

Claim One:
1. A method for obtaining immune repertoire data for an individual, comprising: a) obtaining immunological receptor encoding mRNA from lymphocytes of the individual, wherein the lymphocytes comprise B cells or T cells; b) producing cDNA from the mRNA obtained in step (a); c) producing first amplicons from the cDNA produced in Step (b), wherein the amplicons are produced using a set of primers that amplify a plurality of immunoglobulin heavy chain VDJ exon sequences or that amplify a plurality of T cell receptor beta chain VDJ exon sequences, wherein said amplicons comprise both the VD junction and the DJ junction; d) sequencing first amplicons produced in Step (c) using massively-parallel sequencing to obtain at least 10 4 sequence reads of immunoglobulin heavy chain sequences or at least 10 4 sequence reads of T cell receptor beta chain sequences, comprising sequences from a plurality of different genomic V segments, a plurality of different genomic D segments, and a plurality of different genomic J segments, thereby producing immune repertoire sequence data; e) producing second amplicons from a control library comprising DNA with known immunological receptor sequences, wherein the second amplicons are produced using the primers used in step (c); f) sequencing second amplicons produced in Step (e) using massively-parallel sequencing to obtain at least 10 4 sequence reads of immunoglobulin heavy chain sequences or at least 10 4 sequence reads of T cell receptor beta chain sequences, comprising sequences from a plurality of different genomic V segments, a plurality of different genomic D segments, and a plurality of different genomic J segments, thereby producing control sequence data; g) determining amplification bias and/or sequencing error by comparing the control sequence data with the known sequences, wherein said comparing step comprises compiling sequence data on a computer-readable medium and processing said data on a computer to identify regions of similarity and difference between said sequences; h) normalizing the immune repertoire sequence data based on the amplification bias and/or sequencing error determined in (g), thereby producing corrected immune repertoire sequence data, wherein the corrected immune repertoire sequence data set has reduced amplification bias and/or sequencing error compared to the immune repertoire sequence data of step (d); thereby obtaining immune repertoire data for an individual.