Patent Document ID: 10017734
Application ID: 14910018
Patent Flag: 1

Claim One:
1. A production method of a dopaminergic neuron, comprising subjecting floor plate cells to the following step (1): (1) a step of culturing in a medium containing (i) a cAMP analogue and (ii) a MEK inhibitor, wherein the MEK inhibitor is (i) PD0325901: N-[(2R)-2,3-dihydroxypropoxy]-3,4-difluoro-2-[(2-fluoro-4-iodo-phenyl) amino] benzamide, (ii) PD184352: 2-(2-chloro-4-iodophenylamino)-N-cyclopropylmethoxy-3,4-difluorobenzamide, or (iii) SU5402: 2-[(1,2-dihydro-2-oxo-3H-indol-3-ylidene)methyl]-4-methyl-1H-pyrrole-3-propanoic acid.