Patent Document ID: 9201050
Application ID: 13390885
Patent Flag: 1

Claim One:
1. A method for improving a chromatographic detection limit for an analyte, comprising: a) guiding the analyte through a chromatic separation apparatus; b) producing a chromatogram with a peak of the analyte detected by a detector arranged at an outlet of the chromatic separation apparatus; c) calculating, by a processor, a regression line for a baseline from measured values of a section in the chromatogram without a peak; d) calculating, by the processor, a regression function from measured values of the peak of the analyte, the calculated regression function consisting of a Gaussian function and the calculated regression line for the baseline; e) subtracting the calculated regression function from the chromatogram; f) calculating, by the processor, a regression polynomial for the baseline from values of the chromatogram modified in step e; g) calculating a further regression function from the measured values of the peak in the produced chromatogram, the further regression function consisting of the Gaussian function and the calculated regression polynomial; h) calculating, by the processor, a peak area between the calculated regression polynomial and the calculated further regression function; i) repeating step e), with the further regression function in place of the calculated regression function, and steps f), g) and h) until the calculated peak area changes by less than a preset amount to generate the improved chromatographic detection limit for the analyte; and j) determining a concentration of the analyte based on the calculated peak area.