Patent Document ID: 9448226
Application ID: 13696861
Patent Flag: 1

Claim One:
1. A method of toxicological evaluation of a candidate substance on at least one tissue or organ, comprising the steps of: (a) obtaining a bioartificial tissue or organ by simulation or modeling of the metabolic activity of said tissue or organ by at least one bioreactor; (b) exposing said bioartificial tissue or organ to said substance by introducing said substance inside the at least one bioreactor during said at least one bioreactor functioning; (c) observing the metabolic response of the bioartificial tissue or organ and acquiring, without an a priori method, an associated multidimensional data set; (d) identifying by means of a method of multivariate statistical analysis the components of the multidimensional data set which are quantitatively correlated with predetermined variables; (e) generating a predictive model, consisting of a function between the predetermined variables and the components of the data set actually selected, on the basis of the components of the data set actually selected; (f) testing of the predictive nature of said model by at least one statistical method of estimating reliability; and (g) identifying the metabolic response of the bioartificial tissue or organ in the form of biomarkers associated with the components of the data set selected for the model.