Patent Document ID: 9547014
Application ID: 14125221
Patent Flag: 1

Claim One:
1. A system comprising: a platform comprising one or more channels, at least one channel of said one or more channels comprising: biological assay components suitable to detect the presence of a neural injury biomarker in a biological sample, wherein the neural injury biomarker is an enzyme selected from the group consisting of neuron specific enolase, phosphoglyceromutase, and nucleoside diphosphate kinase A, said components comprising: (i) one or more substrates of the neural injury biomarker and one or more co-factors; and (ii) one or more enzymes capable of acting upon the product of a reaction between the neural injury biomarker and the one or more substrates, wherein at least one enzyme is immobilized within the at least one channel on one or more supports and at least one enzyme is a signal-transducing molecule, said components positioned in the channel to permit a sequential reaction, whereby the neural injury biomarker, if present in the biological sample, will react with the one or more substrates causing a single reaction or a series of coupled reactions that cause production of a measurable signal by the signal-transducing molecule, wherein the measurable signal is selected from the group consisting of a photometrically detectable signal, an electrochemically detectable signal, a colorimetrically detectable signal, and a fluorescent signal.