Patent Document ID: 20010036632
Application ID: 09741983
Patent Flag: 0

Claim One:
1. A method for detecting multiple target polynucleotides in a sample, the method comprising the steps of: a) providing an array of oligonucleotide primers wherein each array contains one or more groups of oligonucleotide primers immobilized on discrete areas of a solid phase support, each group of oligonucleotide primers selectable for a particular target polynucleotide and comprising primer sequences complementary to a sequence of the particular target polynucleotide; b) contacting the array with a reaction mixture comprising a sample, said sample containing or suspected of containing multiple target polynucleotides, said reaction mixture further comprising reagents suitable for polynucleotide hybridization and amplification; c) performing polymerase-mediated polynucleotide amplification for at least two rounds, whereby the target polynucleotides serve as initial templates for the synthesis of detectable nascent polynucleotides which are extended from the immobilized primers; and d) detecting the presence and quantity of the synthesized polynucleotides which are captured on discrete areas of the solid phase support via corresponding immobilized primers.