Patent Document ID: 20060136184
Application ID: 10566953
Patent Flag: 0

Claim One:
1. A method for constructing a variant set for a biopolymer of interest, the method comprising: a) identifying, using a plurality of rules, a plurality of positions in said biopolymer of interest and, for each respective position in said plurality of positions, one or more substitutions for the respective position, wherein the plurality of positions and the one or more substitutions for each respective position in the plurality of positions collectively define a biopolymer sequence space; b) selecting a variant set, wherein said variant set comprises a plurality of variants of said biopolymer of interest and wherein said variant set is a subset of said biopolymer sequence space; c) measuring a property of all or a portion of the variants in said variant set; d) modeling a sequence-activity relationship between (i) one or more substitutions at one or more positions of the biopolymer of interest represented by the variant set and (ii) the property measured for all or said portion of the variants in the variant set; and e) redefining said variant set to comprise variants that include substitutions in said plurality of positions that are selected based on a function of said sequence-activity relationship.