Patent Document ID: 20170166889
Application ID: 15431660
Patent Flag: 0

Claim One:
1. A method for creating a library of polynucleotides, comprising the steps of: providing multiple sets of initial probes, wherein each set of the initial probes comprises an initial forward probe, wherein the initial forward probe (i) is affixed to a bead or a solid surface, and (ii) comprises a sequence complementary to a first subsequence of a first target sequence and one of at least 1,000 unique barcode sequences; introducing the multiple sets of the initial probes into a plurality of compartments, under conditions selected such that more than a half of the plurality of compartments contain one or less than one set of the initial probes; and synthesizing polynucleotides by hybridizing the initial forward probe with the first subsequence of the first target sequence in the plurality of compartments, thereby generating the library of polynucleotides, wherein each of the polynucleotides comprises one of the at least 1,000 unique barcode sequences.