Patent Document ID: 20060246445
Application ID: 10541791
Patent Flag: 0

Claim One:
1. A method for providing an integrated genetic and physical map of a genome or part thereof, the method comprising the steps of: (a) providing at least two individual genetic markers for the genome or part thereof, preferably in the form of a genetic map; (b) characterising the genetic markers by means of at least one AFLP fragment identified through AFLP fingerprinting; (c) providing a library of clones comprising fragments of the genome or part thereof, preferably an artificial chromosome library such as a BAC or YAC; (d) generating a multitude of pools, each pool containing a multitude of individual clones from the library; (e) generating an AFLP fingerprint for each of the pools; (f) identifying in the multitude of pools a pool in which an AFLP fragment identified in (b) is present in the fingerprint of the pool; (g) generating an AFLP fingerprint for each of the individual clones in the pool identified in (f), and identifying the clone containing the AFLP fragment identified in (b) in its AFLP fingerprint; (h) generating a contig comprising the individual clone identified in step (g); (i) repeating steps (f-h) for at least a second AFLP fragment identified in (b) whereby the second or further AFLP fragments characterise a second or further genetic marker; and, (j) linking at least two of the contigs obtained in (h) to thereby obtain an integrated physical and genetic map of the genome or part thereof, which comprises at least two genetic markers; wherein the forward and reverse AFLP primers used in step (b) and (e) comprise K respectively L selective nucleotides at the 3′-end of the primers, wherein the forward and reverse AFLP primers used in step (g) comprise M respectively N selective nucleotides at the 3′-end of the primers, wherein K, L, M, N are integers from 0 to 10, and wherein K+L≧M+N.