Patent Document ID: 9829477
Application ID: 14429035
Patent Flag: 1

Claim One:
1. A planar patch clamp device for analyzing a neuron network, comprising: an electrical insulating plate having a first surface, on which a neuron network is formed, a second surface, which is the opposite side, and a through-hole having a diameter which allows passing through both sides of the plate surface, but does not allow the passage of a cell body; a first liquid pool section formed on the first surface, which enables holding a conductive solution; a second liquid pool section formed on the second surface, which enables holding a pipet solution, and is communicated with the first liquid pool through the through-hole; a first surface side-electrode disposed to be electrically conductive to the conductive solution; and a second surface side-electrode disposed to be electrically conductive to the pipet solution; wherein a cell plating section surrounded by a plurality of protrusions is formed on the first surface so that the protrusions restrict the movement of cells, but allow the formation of a neuron network, the through-hole is arranged in the cell plating section, and the peripheries of the through-hole are coated with an extracellular matrix-forming substance, and wherein each of the electrodes on the first surface side and second surface side comprise: (a) an electrode receptacle for holding each of the electrodes, wherein at least a portion of the receptacle wall that is in contact with the conductive solution or the pipet solution, when the conductive solution or the pipet solution is introduced into the liquid pool sections, is composed of an inorganic porous material; (b) each of the electrodes having a precious metal chloride (NmCl) layer formed on a surface layer section of the precious metal (Nm), and housed in the electrode receptacle; and (c) an electrode solution filled into the electrode receptacle, wherein the precious metal chloride (NmCl) and an alkali metal chloride are dissolved at saturated concentration; and wherein when cells are plated on the cell plating section, both of the first and second surface-side electrodes are not in contact with a cell body.