Patent Document ID: 9506037
Application ID: 13844313
Patent Flag: 1

Claim One:
1. A method for differentiating human embryonic stem cells (hESCs) or human induced pluripotent stem cells (hiPSCs) into early vascular cells (EVCs) in vitro, comprising the steps of: plating a single-cell suspension of hESCs or hiPSCs onto a surface coated with suitable materials selected from the group consisting of type I collagen, fibronectin, and type IV collagen; adding culture medium; culturing the cells for several days; adding Vascular Endothelial Growth Factor (VEGF) and a transforming growth factor-beta (TGF-β) inhibitor to the culture medium; culturing the cells for several days; and harvesting the resulting EVCs, wherein said EVCs express CD73 and one or more of CD105 and CD146.