Patent Document ID: 10018621
Application ID: 13917332
Patent Flag: 1

Claim One:
1. A method for generating a data matrix, comprising: (a) inducing a plurality of signaling pathways in a culture comprising a combination of different human cell types by contacting said culture with two or more factors selected from the group consisting of: TNFα, IL-1, and IFN-γ, wherein said combination comprises primary human endothelial cells; (b) contacting said culture with a test compound, wherein said test compound is selected from the group consisting of: a genetic agent, a chemotherapeutic agent, and an anti-inflammatory agent; (c) measuring the effect of said test compound on assay parameters comprising measurement by immunoassay of IL-8 protein expression, MCP-1 protein expression, and E-selectin protein expression; (d) recording changes in said assay parameters as a result of said contacting; and (e) generating a data matrix from said recording, wherein said data matrix corresponds to said changes in said assay parameters.