Patent Document ID: 20140314299
Application ID: 13865036
Patent Flag: 0

Claim One:
1. A computer-implemented method for performing multi-channel hierarchical image segmentation analysis of tissue types, cell units and sub-cellular units in biological tissue, the method comprising: accessing image data corresponding to multi-channel multiplexed image of biological tissue sequentially fluorescent stained to manifest expression levels of a plurality of morphological biomarkers in the biological tissue; performing multi-channel image segmentation analysis on the image data based on the biomarker expression levels to identify locations and configurations of one or more cells in the biological tissue; performing multi-channel image segmentation analysis on the image data based on the biomarker expression levels to identify locations and configurations of one or more sub-cellular morphological units within the one or more cells; and rendering, on a visual display device, a hierarchical representation of the one or more cells and the one or more sub-cellular morphological units in the one or more cells.