Patent Document ID: 10030219
Application ID: 15011385
Patent Flag: 1

Claim One:
1. A method of fabricating a neurovascular unit (NVU)-on-a-chip, comprising: forming a channel on a chip, passing through an extracellular matrix (ECM) simulation material containing a plurality of types of human-derived brain cells in a gel state; forming a human brain microvessel endothelial cell lining on an intraluminal surface of the channel; stabilizing the brain microvessel endothelial cell lining formed on the intraluminal surface by circulating a culture medium for co-culture in the channel thereby simulating and maintaining a blood brain barrier (BBB) of a human brain constituted by a plurality of human-derived brain tissue cells which are 3-dimensionally positioned around the channel on the chip of the neurovascular unit (NVU), wherein a plurality of types of human-derived brain cells consists of neuron, microglia, astrocyte, pericyte and neuron stem cell.