Patent Document ID: 20100100332
Application ID: 12253676
Patent Flag: 0

Claim One:
1. A computer-implemented method of determining a melting temperature, Tm, of DNA, the method comprising: receiving a dataset representing a melt curve for a DNA sample, the dataset including a plurality of data points each having a pair of coordinate values; numerically determining first derivative values for data points of the melt curve; determining a baseline for the first derivative values; subtracting out the baseline from the first derivative values to produce modified first derivative values; determining a first maximum value of the modified first derivative values; calculating an approximation of a curve that fits the modified first derivative values by applying a Levenberg-Marquardt (LM) regression process to a Gaussian Mixture Model function to determine one or more parameters of the function, wherein said parameters include initial conditions, and wherein the first maximum value is used as an initial condition for a first parameter; and outputting the first parameter, wherein the determined first parameter represents a melting temperature, Tm, of the DNA sample.