Patent Document ID: 20160169915
Application ID: 14903717
Patent Flag: 0

Claim One:
1. A method for identifying a metabolomic signature characteristic for autism in a human, the method comprising: a) assaying a collection of biosamples isolated from autistic subjects for one or a plurality of small molecule metabolites by gas chromatography mass spectrometry (GCMS); b) assaying a collection of biosamples isolated from non-autistic control subjects for one or a plurality of small molecule metabolites by GCMS; c) identifying one or a plurality of small molecule metabolites assayed by GCMS that are differentially produced in autistic subjects as compared to non-autistic control subjects; d) assaying the collection of biosamples isolated from autistic subjects for one or a plurality of small molecule metabolites by one or more untargeted liquid chromatography-high resolution mass spectrometry methodologies (LC/HRMS); e) assaying the collection of biosamples isolated from non-autistic control subjects for one or a plurality of small molecule metabolites by one or more untargeted LC/HRMS methodologies; f) identifying one or a plurality of small molecule metabolites assayed by the one or more untargeted LC/HRMS methodologies that are differentially produced in autistic subjects as compared to non-autistic control subjects; g) combining the plurality of small molecule metabolites identified by step c) and step f) to form a training set of small molecule metabolites; and h) selecting from the training set a subset of small molecule metabolites with a statistically significant abundance difference in the collection of biosamples isolated form autistic patients as compared to the collection of biosamples isolated from control non-autistic control subjects; wherein the subset of small molecules of step h) comprises a metabolomic signature for autism in a human.