Patent Document ID: 20120295819
Application ID: 13458739
Patent Flag: 0

Claim One:
1. A method for amplifying a plurality of different target sequences within a sample, comprising: amplifying within a single amplification reaction mixture at least one hundred different target sequences from a sample including a plurality of different target sequences, wherein the amplifying includes contacting at least some portion of the sample with a plurality of target-specific primers, and a polymerase under amplification conditions, thereby producing at least one hundred different amplified target sequences, wherein at least two of the different amplified target sequences are less than 50% complementary to each other and wherein at least one of the plurality of target-specific primers and at least one of the amplified target sequences includes a cleavable group; cleaving a cleavable group of at least one amplified target sequence; ligating at least one adapter to at least one amplified target sequence in a blunt-ended ligation reaction, thereby producing one or more adapter-ligated amplified target sequences, and reamplifying at least one of the adapter-ligated amplified target sequences.