Patent Document ID: 4219411
Application ID: 05943453
Patent Flag: 1

Claim One:
1. An apparatus for separating a biological cell subpopulation from a biological cell mixture including magnetic functional polymeric particles bound to the cells of said subpopulation comprising in combination: tubular means defining a circuit having an inlet a first and second outlet and a wall portion permeable to a magnetic field disposed in said circuit; first two-way valve means mounted in said circuit and connected to said first outlet; second two-way valve means mounted in said circuit and connected to said second outlet; magnetic field means mounted to generate a magnetic field adjacent said wall portion; a mixing chamber having a particle inlet, a cell suspension inlet, flushing media inlet, recycle inlet and an outlet connected by means of a third valve to the inlet to the circuit; pump means for pumping particle or cell suspension through said circuit; and a recycle conduit connected to said second outlet to the recycle inlet.