Patent Document ID: 9315870
Application ID: 13994732
Patent Flag: 1

Claim One:
1. A method for detecting CpG methylation of GPM6A (glycoprotein M6A) gene, the method comprising the steps of: (a) isolating genomic DNA from a clinical sample of a human subject selected from the group consisting of colorectal tissue, feces, urine, serum, and blood plasma; (b) treating the genomic DNA or a fragment thereof with bisulfite, wherein the genomic DNA or fragment thereof is treated with bisulfite in such a way that cytosine is converted into a base that is different in its base-pairing behavior in the DNA duplex; and (c) determining hypermethylation of the CpG of the promoter or the CpG of the first intron of GPM6A gene in the genomic DNA or fragment thereof treated with bisulfite according to step (b) with primers selected from the group consisting of SEQ ID NOS: 16 to 19.