Patent Document ID: 20090269749
Application ID: 12158040
Patent Flag: 0

Claim One:
1. A method for the high throughput discovery, detection and genotyping of one or more genetic markers in one or more samples, comprising the steps of: (a) providing DNA from one or more samples; (b) restricting the DNA with at least one restriction endonuclease to produce restriction fragments; (c) ligating adaptors to the restriction fragments to produce adaptor-ligated restriction fragments; (d) optionally, amplifying the adaptor-ligated restriction fragments with a primer pair that is complementary to the adaptors to produce pre-amplified adaptor-ligated restriction fragments; (e) amplifying the (optionally pre-amplified) adaptor-ligated restriction fragments with a primer pair, wherein at least one of the primers contains an identifier tag at the 5′ end of the primer to produce a library of tagged amplified subsets of adaptor-ligated restriction fragments for each sample; (f) optionally, pooling the libraries; (g) sequencing the libraries using high throughput sequencing technology; (h) clustering the sequences per library, using the identifier tag; (i) identify genetic markers within the library and/or between libraries (j) determine (co-)dominant genotypes of the genetic markers in one or more libraries.