Patent Document ID: 7920993
Application ID: 11525380
Patent Flag: 1

Claim One:
1. A method for achieving a desired optimal function of a comprehensive biochemical reaction network in a living cell, comprising: (a) representing a listing of the biochemical reactions in the network in a computer; (b) calculating optimal properties of the network under specified environmental conditions by applying a computational optimization method to the list of reactions representing said biochemical reaction network; (c) altering the list of reactions in the network and re-computing the optimal properties; (d) repeating (c) until a desired optimal function is reached under one of the specified environmental conditions, wherein the altered list of reactions providing said desired optimal function determines a set of biochemical reactions; (e) constructing a cell having the genetic makeup containing the biochemical reactions which result from (d); (f) placing the cells constructed under (e) in culture under the specified environmental condition providing the desired optimal function; and (g) cultivating the cells as in step (f) for a sufficient period of time and under the specified environmental condition to allow the cells to evolve to the desired optimal function determined under (d).