Patent Document ID: 9080211
Application ID: 12605337
Patent Flag: 1

Claim One:
1. A method for generating and sequencing a library of DNA fragments having first and second tag sequences, comprising: (a) providing a target DNA; (b) providing a plurality of transposome complexes, wherein (1) at least one transposome complex comprises a hyperactive Tn5 transposase and at least a first polynucleotide comprising a Tn5-type transposon end sequence and a first tag sequence; and (2) at least one transposome complex comprises a hyperactive Tn5 transposase and at least a second polynucleotide comprising a Tn5-type transposon end sequence and a second tag sequence; wherein the transposome complexes of (1) and (2) comprise different sequences; (c) incubating the target DNA and transposome complexes under conditions whereby multiple transposon end sequences and associated tag sequences are inserted into the target DNA and the target DNA is fragmented into a multitude of double-stranded DNA fragments comprising one of the first or the second polynucleotides attached to each 5′ end of the DNA fragments; (d) non-selectively amplifying the double-stranded DNA fragments; and (e) sequencing the DNA fragments simultaneously in a single multiplex format.