Patent Document ID: 8741589
Application ID: 11451735
Patent Flag: 1

Claim One:
1. A method for determining pancreatic β-cell sufficiency in a subject comprising: a) administering one or more isotope-labeled sugars to a subject; wherein said one or more isotope-labeled sugars are metabolized to isotope-labeled and/or unlabeled H 2 O; b) obtaining one or more biological samples from said subject before, during or after the administration of said one or more isotope-labeled sugars, wherein at least one biological sample was obtained after the administration of said one or more isotope-labeled sugars; c) measuring the isotopic content of H 2 O in said one or more biological samples to determine the fractional amount of said isotope-labeled H 2 O in said one or more biological samples; d) determining the total amount of H 2 O in said subject before, during or after the administration of said one or more isotope-labeled sugars; e) multiplying said fractional amount of said isotope-labeled H 2 O in said one or more biological samples by said total amount of H 2 O in said subject to determine the total amount of said isotope-labeled H 2 O in said subject to thereby determine pancreatic β-cell sufficiency in said subject.