Patent Document ID: 20040197309
Application ID: 10481603
Patent Flag: 0

Claim One:
1. A method of inducing bone marrow stromal cells to differentiate into bone marrow stromal cell-derived Schwann cells in vitro, comprising the steps of: (1) collecting bone marrow stromal cells from bone marrow, and culturing said cells in a standard essential culture medium supplemented with a serum; (2) adding a reducing agent to said culture medium, and further culturing said cells; (3) adding a differentiation inducing agent to said culture medium, and further culturing said cells; and (4) adding a cyclic AMP-augmenting agent or a cyclic AMP analogue, and/or a differentiation, survival and growth stimulating factor which acts on nerves and glial cells to said culture medium, and further culturing said cells to obtain said bone marrow stromal cell-derived Schwann cells.