Patent Document ID: 20090061520
Application ID: 11935164
Patent Flag: 0

Claim One:
1. A method for creating a synthetic genetic circuit comprising: a) providing: i) a host cell, ii) a first vector wherein said first vector comprises a selectable marker sequence and wherein said vector is stably integrated into said host cell genome, iii) a second vector comprising a different selectable marker sequence, genetic elements of interest, and first and second homologous sequence that are each homologous to a portion of said selectable marker sequence in said first vector, wherein said first homologous sequence is located 3′ of said different selectable marker sequence and said genetic elements of interest, and said second homologous sequence is located 5′ of said different selectable marker sequence and said genetic elements of interest, and b) applying said second vector to said host cell such that recombination occurs between said selectable marker sequence in said first vector and said first and second homologous sequences thereby causing insertion of said different selectable marker sequence and said genetic elements of interest at a location within said selectable marker sequence of said host cell's genome, thereby generating a synthetic genetic circuit.