Patent Document ID: 20100317103
Application ID: 12303670
Patent Flag: 0

Claim One:
1. A method for inducing the differentiation of neural progenitors from human embryonic stem cells with high efficiency and purity and expanding the same, comprising the steps of: (a) attaching the embryoid bodies cultured for 5 to 21 days, in which the cystic embryoid bodies had been removed, to the surface of culture dishes coated with Matrigel®, laminin, or L-polyornithine, and culturing in media containing 0.5×N-2 supplement for 5 to 7 days to inhibit the growth of other cells than neural cells; (b) dissecting and isolating neural structures from cells derived from attached embryoid bodies expanded for 3 to 7 days in media containing N-2 and bFGF; (c) removing spot-forming fibroblast-like cells and cystic structures, which are generated during the procedure of culturing spherical neural masses derived from the dissected and isolated neural structures, and isolating only the expanded neural structures in the spherical neural masses; and (d) repeating the step (c) at least four times to produce a large number of spherical neural masses with high purity.