Patent Document ID: 7541160
Application ID: 10978701
Patent Flag: 1

Claim One:
1. A method for detecting multiple isoforms of vascular endothelial growth factor (VEGF) in a biological sample, comprising: (a) incubating a biological sample with a capture reagent immobilized on a solid support to bind multiple isoforms of VEGF to the capture reagent at a pH of about 6.0 to about 9.5, wherein the capture reagent comprises a mixture comprising a polyclonal antibody that binds VEGF and a monoclonal antibody that specifically binds to amino acid residues 111-165 of human VEGF; and (b) detecting VEGF bound to the immobilized capture reagent by contacting the bound VEGF with a detectable antibody that binds to N-terminal amino acid residues 1-110 of VEGF.