Patent Document ID: 8748180
Application ID: 13387585
Patent Flag: 1

Claim One:
1. A microfluidic device for culturing cells and/or tissue comprising: a base layer; a cell culture chamber layer comprising one or more cell culture chambers; a fluidic channel layer comprising a plurality of fluid channels; a bottom frame; a gasket; and a top frame, wherein: the cell culture chamber layer is positioned between the fluidic channel layer and the base layer so that the one or more cell culture chambers are fluidically connected to one or more fluid channels of the plurality, the fluid channels have defined geometries that produce one or more desired flow rates through the fluid channels that simulate one or more physiological environments or conditions of interest, the bottom frame has an inlet hole and an outlet hole, the base layer has an inlet hole and an outlet hole, and the cell culture chamber layer has an inlet hole and an outlet hole, and wherein the inlet holes of the bottom frame, the base layer and the cell culture chamber layer and the outlet holes of the bottom frame, the base layer and the cell culture chamber layer align with one another, thereby allowing circulation of fluid through the cell culture chamber layer and the fluidic channel layer.