Patent Document ID: 10053667
Application ID: 15000382
Patent Flag: 1

Claim One:
1. A method of producing p75+ Hnk1+ Ap2+ multipotent neural crest-like cells from human embryonic stem cells (hESCs) or human induced pluripotent stem cells (hiPSCs) comprising differentiating said stem cells in a differentiation medium in the absence of feeder cells consisting essentially of an effective amount of a glycogen synthase kinase (GSK) inhibitor in combination with an effective amount of an Activin A inhibitor to produce a population of cells comprising p75+ Hnk1+ Ap2+ multipotent neural crest-like stem cells and PAX6+ neural progenitor cells, wherein said GSK inhibitor is (2′Z,3′E)-6-Bromoindimbin-3′-oxime (BIO) and said Activin A inhibitor is SB 431542 and wherein said PAX6+ neural progenitor cells produced comprise no more than 10% of the total population of p75+ Hnk1+ Ap2+ multipotent neural crest-like cells and PAX6+ neural progenitor cells; and optionally isolating said neural p75+ Hnk1+ Ap2+ multipotent neural crest-like cells from said population of cells.