Patent Document ID: 9186643
Application ID: 13692615
Patent Flag: 1

Claim One:
1. A method for producing an encapsulated gene product, comprising the steps of: (a) providing within a first microcapsule within a microfluidic channel a first genetic element encoding a first gene product, the microcapsule comprising an aqueous fluid substantially surrounded by an immiscible fluorinated oil including a fluorinated polymer surfactant, wherein the microcapsule is able to contact without fusing with another microcapsule due to presence of the surfactant; (b) providing conditions within the first microcapsule suitable for the expression of the first genetic element to form its first gene product; (c) introducing at least one of a first microbead to the first microcapsule, wherein the first microbead is either encapsulated within the first microcapsule prior to the expression of the first genetic element or is encapsulated within the first microcapsule subsequent to the expression of the first genetic element; and (d) complexing the first gene product with at least one of the plurality of first microbeads via a capture moiety on the first microbead.