Patent Document ID: 20090191547
Application ID: 12077966
Patent Flag: 0

Claim One:
1. A method for detecting one or more sequence changes in a plurality of target nucleic acid sequences in a nucleic acid sample comprising the steps of: a) performing a multiplexed PCR reaction on the nucleic acid sample in the presence of a plurality of target specific PCR primer pairs, wherein each said target specific PCR primer pair comprises a first and second target specific primers and is specific for one of said plurality of target nucleic acid sequences to generate a plurality of amplified products; wherein the first target specific primer comprises a first 5′ region which contains the same sequence for all first target specific primers and a first 3′ region specific for one of the plurality of target nucleic acid sequences; the second target specific primer comprises a first 5′ region which contains the same sequence for all second target specific primers and a second 3′ region specific for one of the plurality of target nucleic acid sequences; b) performing a PCR reaction on said plurality of amplified products with a first and second amplified product specific primer which is specific for said plurality of amplified products; wherein the first amplified product specific primer comprises a 3′ region identical to the first 5′ region of the first target specific primer, the second amplified product specific primer comprises a 3′ region identical to the second 5′ region of the second target specific primer, c) generating homoduplexes and heteroduplexes from the amplified products; d) detecting the presence of the heteroduplexes as an indicator of the one or more sequence changes.