Patent Document ID: 9909180
Application ID: 14172642
Patent Flag: 1

Claim One:
1. A method of identifying an antigen-specific antibody lineage in a subject exposed to the antigen, comprising, sequencing nucleic acid from a biological sample obtained from the subject after exposure to the antigen, via high throughput sequencing, to determine CDR3 nucleic acid sequences, representing at least a portion of the subject's antibody repertoire; performing a linkage analysis of the CDR3 nucleic acid sequences to establish linked CDR3 sequences, wherein two CDR3 sequences are in a linkage if they have the same V and J assignment and no more than one amino acid difference in the CDR3 region; grouping CDR3 sequences into a plurality of lineages, wherein individual lineages of the plurality comprise (i) identical CDR3 sequences and (ii) CDR3 sequences that are associated with one another through one or more linkages; and comparing the CDR3 sequences in the plurality of lineages with a CDR3 sequence of an antigen-specific antibody obtained from the subject to determine the presence of the antigen-specific antibody lineage.