Patent Document ID: 7678579
Application ID: 11784292
Patent Flag: 1

Claim One:
1. A method for detecting characteristic general difference of metabolites between biological sample groups, the method comprising the steps of: extracting metabolites from a biological sample group and a control group without targeting a specific metabolite, by (i) extracting fraction 1 using a solid phase extraction method; (ii) separating fraction 2 from the fraction 1 using a liquid-liquid extraction method after making the fraction 1 buffered at pH 5˜5.5; (iii) separating fraction 3 from leftover liquid phase left after separating the fraction 2 , which is performed using a liquid-liquid extraction method after making the leftover liquid phase hydrolyzed by enzymes; and (iv) separating fraction 4 from leftover liquid phase left after separating the fraction 3 , which is performed using a liquid-liquid extraction method after making the leftover liquid phase buffered at pH 13˜13.5; performing chromatography on each fraction with a chromatography analysis device; converting a result of the chromatography into numerical values capable of being statistically processed; analyzing the numerical values from the biological sample group and the numerical values from the control group with principal component analysis and discriminant analysis to detect comprehensive difference between the numerical values from the biological sample group and the numerical values from the control group; and determining whether there is characteristic difference between the metabolites from the biological sample group and the metabolites from the control group.