Patent Document ID: 8735149
Application ID: 12935427
Patent Flag: 1

Claim One:
1. A method for directing terminal differentiation of neural precursors into motor neurons comprising: providing a population of neural precursors; culturing the neural precursors in a first culturing medium comprising a basic medium supplemented with at least hedgehog (HH) or an HH agonist and with retinoic acid (RA) to obtain neural progenitors having a caudal and ventral specification that is exhibited by at least expression of Ngn2 + ; after obtaining the neural progenitors having a caudal and ventral specification, culturing the neural progenitors having a caudal and ventral specification in a second culturing medium comprising a basic medium supplemented with at least one inhibitor of the Notch signaling pathway, and allowing the neural progenitors having a caudal and ventral specification to terminally differentiate into postmitotic motor neurons expression HB9.