Patent Document ID: 8323898
Application ID: 12194952
Patent Flag: 1

Claim One:
1. A method for identifying pathogenic microorganism responsible for infection, the method comprising: performing gene amplification using one or more of specific primer sets Fungi 1 to Fungi 7 that specifically amplify a gene region common to 18S rRNA genes of fungi to obtain Tm values of the gene region of fungi; performing gene amplification using a specific primer set (spa or spa2) and (mecA or mecA2) that specifically amplifies the Spa gene and the mecA gene of methicillia resistant staphlococcus aureus to obtain Tm values of the Spa gene and the mecA gene; amplifying a gene region of a 16S rRNA gene of bacteria that includes a base sequence region common to bacteria using three or more primer sets including a first primer set, a second primer set, and a third primer set among specific primer sets Bac.1 to Bac.11 to obtain a combination of Tm values of the gene region of known bacteria; storing the Tm values of the gene region of the fungi, the Spa gene, the mecA gene, and the gene region of the known bacteria in a database; subjecting the DNA of an unknown microorganism to gene amplification using the primer sets used to create the database; and selecting known bacteria presumed to be included within an error range of a measured value and a measurement instrument based on the Tm values obtained using the first primer set from the database when it is proved that the unknown microorganism is bacteria based on a combination of Tm values of a specific gene region that has been determined to be positive, the specific gene region having been determined to be negative when being amplified by the specific primer set (spa or spa2) and (mecA or mecA2), and sequentially narrowing a range of the known bacteria included within the error range of the measurement instrument based on the Tm values obtained using the second primer set and the third primer set to identify the unknown microorganism.