Patent Document ID: 9968637
Application ID: 14718683
Patent Flag: 1

Claim One:
1. A method for generating human dopaminergic neurons that coexpress β-tubulin, FOXA2, and tyrosine hydroxlyase (TH), the method comprising: (a) contacting human pluripotent stem cells with about 10 −9 M to about 10 −7 M retinoic acid for at least three days, further contacting them with a WNT1 protein and optionally with 100-1000 ng/mL FGF8A for the at least three days to generate specified neuroectodermal cells; (b) contacting the neuroectodermal cells produced in step (a) 100-1000 ng/mL human sonic hedgehog (SHH) protein and 10-1000 ng/mL FGF8A protein for 18-35 days to generate FOXA2 expressing dopaminergic progenitor cells and (c) culturing the FOXA2 expressing dopaminergic progenitor cells of step (b) in a medium that lacks SHH and FGF8A but comprises brain-derived neurotrophic factor (BDNF), ascorbic acid, cyclic AMP (cAMP), glial-derived neurotrophic factor (GDNF) and transforming growth factor beta 3 (TGF-β3) for at least seven days, thereby generating human dopaminergic neurons expressing β-tubulin, FOXA2, and TH that are capable of structurally and functionally integrating into the host following transplantation.