Patent Document ID: 10039794
Application ID: 14181285
Patent Flag: 1

Claim One:
1. A method of treating a subject with a neurodegenerative disease or disorder comprising: a) producing human parthenogenetic stem cells (hpSCs) by the method comprising: i) parthenogenetically activating a human oocyte by contacting the oocyte with an ionophore at high O 2 tension and contacting the oocyte with a serine-threonine kinase inhibitor under low O 2 tension; ii) cultivating the activated oocyte of (i) at low O 2 tension until blastocyst formation; iii) transferring the blastocyst to a layer of feeder cells, and culturing the transferred blastocyst under high O 2 tension; iv) mechanically isolating an inner cell mass (ICM) from trophectoderm of the blastocyst of (iii); and v) culturing the cells of the ICM of (iv) on a layer of feeder cells, wherein culturing (v) is carried out under high O 2 tension, thereby producing hpSCs; b) treating the hpSCs with SB2180708 and a bone morphogenic protein (BMP) inhibitor; and c) administering the cells into the brain of the subject, thereby treating the neurodegenerative disorder, wherein the neurodegenerative disease or disorder is selected from the group consisting of Parkinson's Disease, stroke, traumatic brain injury, disorders of the substantia nigra and/or dopamine pathway disorders.