Patent Document ID: 20070026411
Application ID: 11193271
Patent Flag: 0

Claim One:
1. A method of preparing labeled RNA from a wax-embedded tissue specimen, the method comprising: digesting the tissue specimen to provide a digested tissue specimen, purifying RNA from the digested tissue specimen to provide a purified RNA fraction, treating the purified RNA fraction with one or more enzymes selected from a cyclic phosphodiesterase, a phosphatase, or a kinase to provide a sample containing dephosphorylated RNA, contacting the sample with an enzyme having an RNA ligation activity in the presence of a labeled substrate under conditions sufficient to result in coupling of the labeled substrate to the RNA in the sample to provide labeled RNA, the conditions including a DMSO concentration in the range from about 20% to about 30%, wherein the labeled substrate comprises an observable label moiety and a nucleotide moiety the nucleotide moiety having a 3′ terminus, the observable label moiety directly attached to the 3′ terminus wherein the observable label moiety is selected from a chromogenic moiety or a fluorophore.