Patent Document ID: 20130143745
Application ID: 13532674
Patent Flag: 0

Claim One:
1. A method for determining essential genetic elements of an organism's genome, the method comprising: performing hyper-saturated transposon mutagenesis using an engineered transposon bearing a first adaptor sequence; selecting for viable mutants under a given selection condition; pooling the selected viable mutants; isolating transposon junctions through a shared parallel PCR strategy, the shared parallel PCR strategy including integrating a second adaptor such that a first adaptor sequence and a second adaptor sequence flank 5′ and 3′ regions of a sample DNA, respectively; sequencing the sample DNA in an ultra high-throughput adapter based DNA sequencer; and mapping transposon insertion sites onto the organism's genome; and identifying the essential genetic elements based on frequency, locations and insertion orientation of the mapped transposon insertion sites.