Patent Document ID: 9982279
Application ID: 15632001
Patent Flag: 1

Claim One:
1. A nucleic acid-guided nuclease system comprising: (a) a nucleic acid-guided nuclease comprising the amino acid sequence of SEQ ID NO: 7, wherein the nucleic-acid guided nuclease is encoded by a wild-type or a codon-optimized nucleic acid; (b) an engineered guide nucleic acid capable of complexing with the nucleic acid-guided nuclease, wherein the engineered guide nucleic acid comprises any one of SEQ ID NO: 88, SEQ ID NO: 93, and SEQ ID NO: 94, and (c) an editing sequence having a change in sequence relative to the sequence of a target region in a genome of a cell and a mutation in a protospacer adjacent motif (PAM) site; wherein the system results in a genome edit in the target region in the genome of the cell facilitated by the nuclease, the engineered guide nucleic acid, and the editing sequence.