Patent Document ID: 6013456
Application ID: 09245984
Patent Flag: 1

Claim One:
1. A method for determining the base sequence of a portion of a single stranded nucleic acid by determining the base sequence of a complementary strand comprising the steps of: a) providing a reaction mixture comprising a single stranded nucleic acid, a capture probe/primer which is complementary to a portion of the single stranded nucleic acid, and a plurality of labeled identical length oligonucleotide 5'-monophsphates wherein each opligonucleotide 5'-monophophate compxises a label that is specific for and identifies that oligonucleotide 5'-monophosphate; b) hybridizing the capture probe/primer with the single stranded nucleic acid to form a captured probe-nucleic acid hybrid; c) ligating more than one of the plurality of the oligonucleotide 5'-monophosphates in a contiguous manner onto the capture probe/primer in one continuous process to synthesize the complementary strand, wherein ligation of oligonucleotide 5'-monophosphates only occurs in the presence of the hybridized primer; d) removing all nonligated oligonucleotide 5'-monophosphates; e) detecting the plurality of labels which were attached in the ligation; f) relating the plurality of detected labels to the identity of their corresponding oligonucleotide 5'-monophosphates; and g) determining the base sequence of the portion of the nucleic acid from the identity of the plurality of oligonucleotide 5'-monophosphates identified in step f.