Patent Document ID: 20040005713
Application ID: 10444664
Patent Flag: 0

Claim One:
1. A method for expressing a nucleotide sequence of interest in a plant cell comprising, a) providing an Agrobacterium replicon having a T-DNA, wherein said T-DNA comprises in a, 5′ to 3′ or a 3′ to 5′ orientation, a first target site for a site-specific recombinase, a viral replicon, said nucleotide sequence of interest operably linked to a first promoter that drives expression in said plant cell, and a second target site for said site-specific recombinase, wherein said first and said second target site are directly repeated and identical with respect to one another; b) infecting said plant cell with an Agrobacterium carrying said Agrobacterium replicon under conditions that allow transfer of said T-DNA and expression of said site-specific recombinase in said plant cell; and, wherein said plant cell, said T-DNA, or said viral replicon comprises a nucleotide sequence encoding said site-specific recombinase, an active fragment of said site-specific recombinase, or an active variant of said site-specific recombinase, wherein said nucleotide sequence is operably linked to a second promoter that drives expression in said plant cell, and wherein said active fragment or said active variant of said site-specific recombinase retains the ability to catalyze a recombination event.