Patent Document ID: 20140172318
Application ID: 14134353
Patent Flag: 0

Claim One:
1. A method for identifying and optimizing metabolic flux states comprising: providing a set of reactions for a cellular phenotype; identifying a set of enzymes that participate in the set of reactions for the cellular phenotype; providing the metabolic costs associated with production of each enzyme of the set of enzymes that participates in the set of reactions for the cellular phenotype; identifying a first pool of amino acids which participate in the set of reactions for the cellular phenotype; identifying a second pool of amino acids which are constituents of the set of enzymes that participate in the set of reactions for the cellular phenotype; maximizing the cellular phenotype by obtaining a maximum flux of the first pool of amino acids through the set of reactions such that all possible fluxes corresponding to the maximized cellular phenotype are identified; and obtaining a minimum-cost flux distribution of the second pool of amino acids for each of the possible fluxes corresponding to the maximized cellular phenotype based on the provided metabolic costs; whereby metabolic flux states that maximize the cellular phenotype are identified and metabolic costs associated with production of the set of enzymes that participate in the set of reactions for the cellular phenotype are minimized.