Patent Document ID: 9606109
Application ID: 14850628
Patent Flag: 1

Claim One:
1. A method of assessing infection or risk for an infection with a bacteria or a virus, comprising: contacting a first sample comprising T-cells and B-cells obtained from a patient with an infection or at risk for infection with the bacteria or the virus with an antigen from the bacteria or the virus, respectively, under conditions sufficient to induce cell proliferation by the antigen; contacting a second sample comprising T-cells and B-cells obtained from the patient with an infection or at risk for infection with a third-party antigen under conditions sufficient to induce cell proliferation by the third-party antigen not from the bacteria or virus; measuring T-memory cell proliferation and T helper cell proliferation induced by the antigen from the bacteria or the virus by detecting a plurality of markers in the first sample following contacting the first sample with the antigen from the bacteria or the virus, respectively, wherein the plurality of markers comprises CD154, CD8, at least one T helper cell marker, at least one T-memory cell marker and one T-cell marker; measuring T-memory cell proliferation and a T helper cell proliferation by the third party antigen by detecting a plurality of markers in the second sample following contacting the second sample with the third-party antigen, wherein the plurality of markers comprises CD154, CD8, at least one T helper cell marker, at least one T-memory cell marker and one T-cell marker, comparing T-memory cell proliferation or T helper cell proliferation induced by the antigen from the bacterial or the virus in the first sample to T-memory cell proliferation or T helper cell proliferation induced by viable third-party antigen in the second sample, wherein decreased T-memory cell proliferation or T helper cell proliferation in the first sample as compared to the second sample indicates that the patient has the infection or is at risk for the infection.