Patent Document ID: 20120245036
Application ID: 13298559
Patent Flag: 0

Claim One:
1. A method of screening, evolving and expressing of an antibody, or antibody fragment, in a eukaryotic cell production host; the method comprising: a. screening an antibody library generated from a eukaryotic cell production host with antibody cell surface display for at least one predetermined property, characteristic or activity, b. evolving a template antibody from the library to produce a set of mutant antibodies in the eukaryotic cell production host; c. screening the mutant antibodies for the at least one predetermined property, characteristic or activity under screening conditions that mimic manufacturing or formulation conditions; and d. expressing an up-mutant antibody obtained from step c. in the eukaryotic cell production host; wherein the eukaryotic cell production host in a. and d. is the same.