Patent Document ID: 9103826
Application ID: 13490360
Patent Flag: 1

Claim One:
1. A method for differentiating dengue disease severity in an individual having a dengue virus infection, the method comprising: (1)-determining the presence and quantity of or absence of high molecular weight albumin*2, high molecular weight albumin*3, IL-10, fibrinogen, complement 4A (C4A), immunoglobulin, tropomyosin, and albumin*1 in a plasma sample from the individual by (a) contacting a plasma sample from the individual with probes that specifically bind to high molecular weight albumin*2, high molecular weight albumin*3, IL-10, fibrinogen, complement 4A (C4A), immunoglobulin, tropomyosin, or albumin*1, wherein a complex forms comprising the specific probe and high molecular weight albumin*2, high molecular weight albumin*3, IL-10, fibrinogen, complement 4A (C4A), immunoglobulin, tropomyosin, and albumin*1, if present in the sample, and (b) measuring the amount of specific probe bound to the high molecular weight albumin*2, high molecular weight albumin*3, IL-10, fibrinogen, complement 4A (C4A), immunoglobulin, tropomyosin, and albumin*1; and (2) identifying the individual's risk of developing dengue hemorrhagic fever (DHF) based on a multivariate adaptive regression splines (MARS) classifier of DHF that includes basis functions of IL-10, fibrinogen, C4A, immunoglobulin, tropomyosin, albumin*1, albumin*2 and albumin*3.