Patent Document ID: 9625462
Application ID: 14814139
Patent Flag: 1

Claim One:
1. An immunoassay kit for selectively detecting VEGF 110+ forms in a biological sample, the forms including VEGF 121 , the kit comprising: (a) as capture reagent, a monoclonal antibody against human VEGF comprising the six complementarity-determining regions (CDRs) of monoclonal antibody 5C3, obtainable from hybridoma 5C3.1.1 deposited at the American Type Culture Collection (ATCC) under accession number PTA-7737, wherein the monoclonal antibody binds specifically to the residues greater than 110 of human VEGF and binds VEGF 121 ; and (b) as detection reagent, a detectable antibody comprising the six CDRs of monoclonal antibody A4.6.1, obtainable from hybridoma A4.6.1 deposited at ATCC under accession number HB10709, wherein said detectable antibody binds to the KDR and/or FLT1 receptor binding domains of VEGF or that binds to an epitope in VEGF 1-110.