Patent Document ID: 8093053
Application ID: 12405736
Patent Flag: 1

Claim One:
1. A culture medium comprising, dissolved or dispersed in base culture media and water, the following components: (a) nutrients; (b) glutamine; (c) glucose; (d) heparin (e) Hepes; (f) recombinant human EGF at about 10 to about 40 μg/mL; (g) recombinant human bFGF about 10 to about 40 μg/mL; (h) recombinant hLIF at about 5 to about 25 μg/mL; (i) DHEA at about 0.1 to about 5 μmol/L; (j) serum albumin; (k) lipids; and (l) a mixture of hormones comprising apo-transferrin, insulin, putrescine, selenium and progesterone.