Patent Document ID: 20030113750
Application ID: 10172086
Patent Flag: 0

Claim One:
1. A method for determining genetic and/or epigenetic parameters for the classification, differentiation and/or diagnosis of prostate tumours or the predisposition to prostate cancer by analysing cytosine methylations, characterised in that the following steps are carried out: a. obtaining a biological sample containing genomic DNA b. extracting the genomic DNA c. in the genomic DNA sample, cytosine bases which are unmethylated at the 5-position are converted, by chemical treatment, to uracil or another base which is dissimilar to cytosine in terms of hybridisation behaviour; and d. amplifying at least one fragment of the chemically pretreated genomic DNA using sets of primer oligonucleotides and a polymerase, wherein the genomic CpG sequences are located within at least one of the chemically pretreated genomic sequences according to Seq. ID No.1 to Seq. ID No.112, and sequences complementary thereto.