Patent Document ID: 7595194
Application ID: 11415056
Patent Flag: 1

Claim One:
1. A method of isolating a pure population of mammalian CNS glial-restricted precursor cells wherein said cells are capable of self-renewal in adherent feeder-cell-independent culture medium and of differentiation to CNS glial cells but not CNS neuronal cells, comprising the steps of: (a) isolating a population of mammalian CNS neuroepithelial stems cells; (b) incubating the neuroepithelial stem cells in a medium lacking an effective amount of chick embryo extract for a period of time sufficient for the cells to begin differentiating; (c) subjecting the incubated cells to specific antibody capture using an A2B5 antibody characteristic of glial-restricted precursor cells to result in a captured subpopulation of cells; and (d) incubating the captured subpopulation of cells in a medium configured for supporting adherent growth thereof comprising effective amounts of fibroblast growth factor and platelet derived growth factor.