Patent Document ID: 8906620
Application ID: 12077966
Patent Flag: 1

Claim One:
1. A method for detecting one or more sequence changes in a plurality of target nucleic acid sequences in a nucleic acid sample comprising the steps of: a) performing a multiplexed PCR reaction on the nucleic acid sample in the presence of a plurality of target specific PCR primer pairs, wherein each said target specific PCR primer pair comprises a first and a second target specific primer and is specific for one of said plurality of target nucleic acid sequences to generate a plurality of amplified products; wherein said first target specific primer comprises a first 5′ region which contains the same sequence for all first target specific primers of said plurality of target specific PCR primer pairs and a first 3′ region specific for said one of the plurality of target nucleic acid sequences; said second target specific primer comprises a second 5′ region which contains the same sequence for all second target specific primers of said plurality of target specific PCR primer pairs and a second 3′ region specific for said one of the plurality of target nucleic acid sequences, and wherein said first 5′ region of said first target specific primer and said second 5′ region of said second target specific primer are different from each other; b) performing a PCR reaction on said plurality of amplified products with a first and a second amplified product specific primer which are specific for said plurality of amplified products; wherein the first amplified product specific primer comprises a 3′ region identical to the first 5′ region of the first target specific primer, the second amplified product specific primer comprises a 3′ region identical to the second 5′ region of the second target specific primer; c) generating homoduplexes and heteroduplexes from the amplified products; and d) detecting the presence of the heteroduplexes as an indicator of the one or more sequence changes.