The Effect of Aerobic and Anaerobic Exercise on Oxidative Stress and Cellular Fitness in Healthy Trained Young Men
NCT Number: NCT06582862
Status: ENROLLING_BY_INVITATION
Study Type: INTERVENTIONAL
Phases: NA
Conditions: Health, Subjective
Interventions: OTHER: Exercise
Sponsor: Indonesia University
Sex: MALE
Age: ADULT
Enrollment: 20
Study Design: Allocation: RANDOMIZED|Intervention Model: PARALLEL|Masking: NONE|Primary Purpose: BASIC_SCIENCE
Start Date: 2024-06-01
Completion Date: 2024-11-30
Locations: Faculty of Medicine Universitas Indonesia, Jakarta Pusat, Jakarta, 10430, Indonesia

Summary:
This study aims to analyze the effects of aerobic and anaerobic exercise on health at the molecular level. By examining these effects, the study seeks to provide the public with insights into which types of exercise offer the most significant health benefits. Participants will engage in aerobic and anaerobic exercises for one month, with 5 mL of venous blood collected by experienced phlebotomists both before and after the exercise period. While blood collection may cause mild discomfort and temporary bruising, these symptoms should subside within a few days. The collected blood will be processed to separate plasma and leukocytes for the assessment of oxidative damage, lipid profile and cellular fitness parameters. The oxidative damage markers to be measured include malondialdehyde (MDA) levels, H2O2, and antioxidants such as total antioxidant capacity, superoxide dismutase, and glutathione peroxidase. Cellular fitness will be evaluated by measuring mitochondrial biogenesis markers (succinate dehydrogenase and PGC-1A), ATP levels, and ATPase inhibitor levels. The benefits for the participants, they will receive include a laboratory assessment to evaluate cell damage, lipid profiles, and mitochondrial function. Additionally, the study results will help identify the most beneficial type of physical exercise for optimal health.

Primary Outcome Measures: Concentration of Malondialdehyde (MDA), MDA in nmoL/mL is measured using spectrophotometer from the subject's plasma, 1 month|Concentration of Hydrogen Peroxide, Hydrogen peroxide in mmol/L is measured using spectrophotometer from the subject's plasma, 1 month|Concentration of Superoxide dismutase (SOD), SOD in U/mL is measured using spectrophotometer from the subject's plasma, 1 month|Concentration of Total Antioxidant Capacity, Total Antioxidant Capacity in U/mL is measured using spectrophotometer from the subject's plasma, 1 month|Concentration of Glutathione Peroxidase (GPx), GPx in in U/mL is measured using spectrophotometer from the subject's plasma, 1 month
Secondary Outcome Measures: Concentration of Succinate Dehydrogenase (SDH), SDH in U/L is measured using spectrophotometer from the peripheral blood mononuclear cells of subject, 1 month|Concentration of Peroxisome proliferator-activated receptor-gamma coactivator (PGC)-1alpha, PGC-1alpha in ng/mL is measured using spectrophotometer from the the peripheral blood mononuclear cells of subject, 1 month|Concentration of Adenosine triphosphate (ATP), ATP in micromole/L is measured using spectrophotometer from the the peripheral blood mononuclear cells of subject, 1 month|Concentration of ATPase inhibitor, ATPase inhibitor in ng/mL is measured using spectrophotometer from the the peripheral blood mononuclear cells of subject, 1 month