Vitamin and Unsaturated Fatty Acid Supplements in Healthy and Uveitis Human Subjects
NCT Number: NCT06491979
Status: RECRUITING
Study Type: INTERVENTIONAL
Phases: NA
Conditions: Healthy|Uveitis|Autoimmune Diseases
Interventions: DIETARY_SUPPLEMENT: Vitamin A treatment|DIETARY_SUPPLEMENT: Monounsaturated fatty acid mixture|DIETARY_SUPPLEMENT: Polyunsaturated fatty acid mixture|DIETARY_SUPPLEMENT: Fatty acid mixture
Sponsor: Tianjin Medical University
Sex: ALL
Age: ADULT, OLDER_ADULT
Enrollment: 200
Study Design: Allocation: NON_RANDOMIZED|Intervention Model: SINGLE_GROUP|Masking: NONE|Primary Purpose: BASIC_SCIENCE
Start Date: 2024-07
Completion Date: 2026-12
Locations: Tianjin Medical University Eye Hospital, Tianjin, Tianjin, 300071, China

Summary:
The goal of this clinical trial is to learn if dietary supplements containing vitamin and unsaturated fatty acid works to intervene T cell inflammatory factor expression in patients with uveitis and healthy subjects in adults.

Primary Outcome Measures: Inflammatory factors-Interleukin-17A (IL-17A), Flow cytometry analysis of peripheral blood mononuclear cells (PBMCs) to detect the ratio of Interleukin-17A (IL-17A) -producing cells in CD4+ T cells and CD8+ T cells, 3 weeks|Inflammatory factors-Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF), Flow cytometry analysis of peripheral blood mononuclear cells (PBMCs) to detect the ratio of Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) -producing cells in CD4+ T cells and CD8+ T cells, 3 weeks|Inflammatory factors-Interferon gamma (IFN-γ), Flow cytometry analysis of peripheral blood mononuclear cells (PBMCs) to detect the ratio of Interferon gamma (IFN-γ) -producing cells in CD4+ T cells and CD8+ T cells, 3 weeks|Inflammatory factors-Interleukin-2 (IL-2), Flow cytometry analysis of peripheral blood mononuclear cells (PBMCs) to detect the ratio of Interleukin-2 (IL-2) -producing cells in CD4+ T cells and CD8+ T cells, 3 weeks|Inflammatory factors-Interleukin-4 (IL-4), Flow cytometry analysis of peripheral blood mononuclear cells (PBMCs) to detect the ratio of Interleukin-4 (IL-4) -producing cells in CD4+ T cells and CD8+ T cells, 3 weeks|Inflammatory factors-Interleukin-9 (IL-9), Flow cytometry analysis of peripheral blood mononuclear cells (PBMCs) to detect the ratio of Interleukin-9 (IL-9) -producing cells in CD4+ T cells and CD8+ T cells, 3 weeks|Inflammatory factors-Interleukin-10 (IL-10), Flow cytometry analysis of peripheral blood mononuclear cells (PBMCs) to detect the ratio of Interleukin-10 (IL-10) -producing cells in CD4+ T cells and CD8+ T cells, 3 weeks|Inflammatory factors-Tumor Necrosis Factor alpha (TNF-α), Flow cytometry analysis of peripheral blood mononuclear cells (PBMCs) to detect the ratio of Tumor Necrosis Factor alpha (TNF-α) -producing cells in CD4+ T cells and CD8+ T cells, 3 weeks
Secondary Outcome Measures: Cytokine levels-Interleukin-17A (IL-17A), Analysis of cytokine levels in blood. Detect the concention of Interleukin-17A (IL-17A) in plasma, 3 weeks|Cytokine levels-Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF), Analysis of cytokine levels in blood. Detect the concention of Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) in plasma, 3 weeks|Cytokine levels-Interferon gamma (IFN-γ), Analysis of cytokine levels in blood. Detect the concention of Interferon gamma (IFN-γ) in plasma, 3 weeks|Cytokine levels-Interleukin-2 (IL-2), Analysis of cytokine levels in blood. Detect the concention of Interleukin-2 (IL-2) in plasma, 3 weeks|Cytokine levels-Interleukin-10 (IL-10), Analysis of cytokine levels in blood. Detect the concention of Interleukin-10 (IL-10) in plasma, 3 weeks|Cytokine levels-Interleukin-9 (IL-9), Analysis of cytokine levels in blood. Detect the concention of Interleukin-9 (IL-9) in plasma, 3 weeks|Cytokine levels-Interleukin-4 (IL-4), Analysis of cytokine levels in blood. Detect the concention of Interleukin-4 (IL-4) in plasma, 3 weeks|Cytokine levels-Tumor Necrosis Factor alpha (TNF-α), Analysis of cytokine levels in blood. Detect the concention of Tumor Necrosis Factor alpha (TNF-α) in plasma, 3 weeks
Other Outcome Measures: Transcriptomics analysis, Analyze the changes in peripheral blood mononuclear cells using transcriptomics. Detect the cell populations , genes number and expression., 3 weeks|Proteomics analysis, Analyze the changes in peripheral blood mononuclear cells using proteomics. Detect the proteins expression., 3 weeks|post-translational modification proteomics analysis, Analyze the changes in peripheral blood mononuclear cells using post-translational modification proteomics. Detect the protein various modification, such as lactylation, crotonylation, palmitoylation and methylation., 3 weeks|metabolomics analysis, Analyze the changes in peripheral blood mononuclear cells using metabolomics. Detect the metabolites numbers, localization and level., 3 weeks