Molecular Optimization Via Exercise (MOVE)
NCT Number: NCT06540287
Status: NOT_YET_RECRUITING
Study Type: OBSERVATIONAL
Conditions: Aging
Interventions: OTHER: Observational
Sponsor: Buck Institute for Research on Aging
Sex: ALL
Age: OLDER_ADULT
Enrollment: 80
Study Design: Observational Model: |Time Perspective: p
Start Date: 2024-08
Completion Date: 2027-05
Locations: Buck Institute for Research on Aging, Novato, California, 94945, United States

Summary:
This study is to investigate characteristics of muscle, blood, and urine samples from older adults (ages 65-80) who are either sedentary or athletes performing at an elite level for their age. This observational study will include bio-specimen collection, as well as physical and cognitive function testing and body composition.

Primary Outcome Measures: Muscle cellular aging - spatial transcriptomics, Differences between athletes and sedentary in muscle spatial transcriptomic between OEA and HOA groups, Day 2|Muscle cellular aging - single cell transcriptomics, Differences between athletes and sedentary in muscle single cell transcriptomic between OEA and HOA groups, Day 2
Secondary Outcome Measures: Differences between concentration of proteins in the muscle, Proteomic analysis to measure differences in protein abundance in EOA vs HOA, Day 2|Differences between concentration of metabolites in the muscle, Metabolomic analysis to measure differences in metabolite abundance in EOA vs HOA, Day 2|Differences between function of muscle mitochondria, Analysis of respiratory function of isolated mitochondria, Day 2|Blood metabolites, Metabolic analysis: differences between athletes and sedentary in blood metabolites, Day 2|Blood proteins, Proteomic analysis: differences between athletes and sedentary in blood protein concentration, Day 2|Blood cell immune function, Differences between athletes and sedentary in blood cell immune function, Day 2|Blood cell gene expression, Differences between athletes and sedentary in blood cell gene expression, Day 2|Urine metabolites, Differences between athletes and sedentary in urine metabolites, Day 2|Physical function - VO2 max, Oxygen uptake measured during a ramp test on a bicycle ergometer, Day 1|Physical function - grip strength, Measured in both hands using a dynamometer, Day 1|Physical function - 6 minute walk test, Measure distance covered in 6 minutes on an indoor walk track, Day 1|Physical function - short physical performance battery, SPBB includes balance, gait speed and chair sit tests, consolidated to one score, Day 1|Physical function - leg strength, Maximal leg press weight will be determined on a gym machine via progressive increases in weight, Day 1|Physical function - leg press fatigue, 70% of the maximal leg press weight will be used. Subjects will complete as many repetitions as possible of the 70% weight and number of repetitions will be recorded., Day 1
Other Outcome Measures: Body composition - BIA, Body composition measured by bioelectrical impedance, Day 1|Body composition - muscle mass, Total muscle mass measured by D3 creatine supplement followed by urine collection, Day 2|Cognitive function - Montreal Cognitive Assessment, Paper and interview based test that measures multiple domains of function, Day 1|Cognitive function - digit symbol substitution, Paper based test that involves matching symbols and numbers in a set time., Day 1|Cognitive function - trails A and B, Paper based test that involves joining numbers or numbers and letters in series, as fast as possible., Day 1|Social determinants of health, Assessed by Upstream Risks paper based self-complete questionnaire, Day 1|Sleep quality questionnaire, Pittsburgh Sleep Quality Index assessed by paper based self-complete questionnaire, Day 1|Overal function perception, SF-36 (Short Form 36) validated questionnaire (paper based, self complete) that determines ease of completion of different life activities., Day 1|Depression, Geriatric Depression Scale (GDS) validated questionnaire (paper based, self complete) that assesses presence of depression symptoms, Day 1