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Article:The Eaton Place surgery closed its doors for the last time at lunchtime because its two partners are retiring.
Local Conservative MP Simon Kirby, who had campaigned for it to remain open, said he was very disappointed.
NHS England said patients would be able to register with other nearby practices at a drop-in event next week.
At least half the patients have already registered with one of the 13 surgeries closest to Eaton Place.
NHS England has offered the surgeries a payment of Â£25 per head for every patient they take.
Mr Kirby, the MP for Brighton Kemptown and Peacehaven, had hoped another GP would take on the practice and said discussions had been so advanced that the recruitment process for new staff had already begun.
"It is particularly saddening that the final discussions between these various groups have not been successful," he said.
Patients wishing to register with another GP can visit the Wellsbourne Health Centre, in Whitehawk, on 4 March between 10:00 and 14:00 GMT.

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Answer:
Six thousand patients in Brighton have been left trying to find a new doctor after talks to safeguard the future of their surgery broke down.

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Article:Members of Cymdeithas yr Iaith are taking the action as part of a campaign to get the powers transferred to the Welsh Assembly.
Chairwoman Heledd Gwyndaf said she was prepared to be jailed rather than pay for her licence until powers were devolved.
TV Licensing said the campaigners risked prosecution and a £1,000 fine.
"It's not an easy decision to make, we're obviously breaking the law," said Ms Gwyndaf of her refusal to pay the £147-a year licence.
"I have done this for many months now. I have a young family, I have three small children.
"I have received many letters telling me that I need to pay, telling me that a bailiff is on the way, giving me a date as to when the bailiffs will arrive."
But she said she believed devolving broadcasting was important to the Welsh language and to Wales as a nation.
"In other countries where broadcasting is already devolved, for example in Catalonia and the Basque country, they have six or eight radio stations and television stations in their mother language, or that broadcast bilingually," she said.
"There's no reason why we cannot devolve broadcasting to Wales."
The Department for Culture, Media and Sport said it could not comment because of the impending general election.
Labour's Welsh Government minister Alun Davies, who has responsibility for broadcasting, said he favoured greater accountability - but not full devolution.
"I don't think there's a groundswell of opinion across Wales for executive responsibility for broadcasting to be devolved to Wales," he said.
"But I think there is concern across Wales about what we see on our screens, and what sort of services we receive. And what I would like to see is a greater sense of accountability from broadcasters and regulators to Wales, and to the institutions of Wales."
He said he wanted to see broadcasters become more accountable to assembly members.
"We all know that we don't see enough programming made in Wales, we don't see the portrayal of Wales on our screens in a way that we deserve and should see, and I think it's time that both regulators and broadcasters came to Cardiff and explained what they are doing to serve Welsh audiences," he added.
Plaid Cymru and the Liberal Democrats support the devolution of broadcasting. The Conservatives favour shared accountability but not full devolution, while UKIP do not support any devolution of broadcasting powers.
In a statement, TV Licensing said: "Regardless of personal opinion, a TV Licence is required to watch or record TV programmes as they are broadcast, or watch BBC programmes on iPlayer."

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Answer:
More than 50 Welsh language campaigners are refusing to pay their TV licences until broadcasting powers are devolved.

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Article:The programme began in 2013 as Mr Trump's predecessor, Barack Obama, decided to put pressure on President Bashar al-Assad to stand down.
The decision to halt it was made almost a month ago, by which time the flow of arms had already slowed significantly.
The officials said the move was part of an effort to improve ties with Russia.
Russia's military support has helped Mr Assad hold on to power during the six-year civil war that has left more than 300,000 people dead and displaced 11 million others.
The Washington Post newspaper was the first to report the end of the CIA weapons programme on Wednesday night.
Mr Trump made his decision after a meeting in the Oval Office with National Security Adviser H R McMaster and CIA Director Mike Pompeo and before he held talks with Russian President Vladimir Putin at the G20 summit in Germany on 7 July, according to the Post.
Two days after the summit, a truce brokered by the US and Russia took effect in three southern Syrian provinces. But the end of the weapons programme was reportedly not a condition of the negotiations.
US officials told Reuters news agency there had long been doubts about the programme's effectiveness. Despite a lengthy vetting process, some rebels had defected to so-called Islamic State (IS) and other jihadist groups, they said.
The CIA-backed groups have also been targeted repeatedly by Russian air strikes, which began in September 2015.
One official said Mr Trump's decision was not a major concession to Russia given Mr Assad's grip on power, adding: "It's a signal to Putin that the administration wants to improve ties."
But another called the decision "momentous", telling the Post: "Putin won in Syria."
A separate US military programme to train, arm and provide air support to an alliance of Kurdish and Arab fighters battling so-called Islamic State will continue.
The White House and the CIA declined to comment on the reports.
Mr Trump had suggested before he took office in January that he might end support for the rebels and prioritise the fight against IS in eastern and northern Syria.
In late March, the White House said it had abandoned the goal of forcing Mr Assad to stand aside, explaining that "there is a political reality that we have to accept".
But the next month, after the US accused the Syrian government of a deadly chemical weapons attack on a rebel-held town, Mr Trump ordered a cruise missile strike on a Syrian air base and his secretary of state said Mr Assad's actions suggested "there would be no role for him to govern the Syrian people".
Mr Trump's decision to agree to the Russian demand to end the weapons programme comes as he faces intense scrutiny by Congress and a special counsel investigating alleged Russian interference in the 2016 US presidential election.

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assistant: US President Donald Trump has ended the CIA's clandestine programme to provide weapons and supplies to Syrian rebel groups, officials have said.