AGRIS - 

2023

Báez, Wilfrido Javier Arrúa | Quintana, Juan Rafael Centurión | Montalbetti Moreno†, Yenny | Almada, Olga Yolanda Heinichen

Resumen: Animales de laboratorio criados en diferentes Bioterios muestran variaciones en sus características fisiológicas debido a la dieta, manejo, estado de salud, edad, vías de toma de muestras y otros factores. Por ello la importancia de determinar el perfil bioquímico de ratones y ratas convencionales del Bioterio de la Facultad de Ciencias Químicas de la Universidad Nacional de Asunción (FCQ-UNA). En el estudio experimental se utilizaron 60 ratones Swiss y 60 ratas Wistar, machos y hembras, de 11 semanas de edad. Se determinaron distintos parámetros bioquímicos como los perfiles renal, hepático, lipídico y proteico, electrolitos, glicemia y hemoglobina glicosilada. Los animales no fueron expuestos a tratamientos químicos o farmacológicos que pudieran cambiar su estado fisiológico natural. El análisis comparativo de los resultados con los presentados en la literatura, demostraron claramente la existencia de variaciones intra-especie. Las diferencias más notorias se observaron en el perfil hepático; ya que, los niveles de transaminasas séricas de los animales presentaron valores por debajo de la establecida en la bibliografía consultada. Además, claramente se evidenció valores inferiores de glicemia en los animales del Bioterio de la FCQ-UNA comparados con Bioterios de la región. Este estudio estandarizó el perfil bioquímico de ratas y ratones convencionales del Bioterio de la Facultad de Ciencias Químicas de la UNA, ofreciendo una herramienta valiosa a los investigadores para el análisis de sus resultados experimentales. | Abstract: Laboratory animals reared in different Animal Facilities show variations in their physiological characteristics due to diet, management, health status, age, sampling routes, and other factors. For this reason, the importance of determining the biochemical profile of conventional mice and rats from the Animal Facility of the Facultad de Ciencias Químicas of the Universidad Nacional de Asunción (FCQ-UNA). In the experimental study, were used 60 Swiss mice and 60 Wistar rats, males and females, 11 weeks old. Were determined different biochemical parameters such as renal, liver, lipid, and protein profiles, electrolytes, glycemia and glycosylated hemoglobin. The animals were not exposed to chemical or pharmacological treatments that could change their natural physiological state. The comparative analysis of the results with those presented in the literature clearly demonstrated the existence of intra-species variations. The most noticeable differences were observed in the liver profile; since the serum transaminase levels of the animals presented values below that established in the consulted bibliography. In addition, lower glycemia values were clearly evidenced in the animals of the Animal Facility of FCQ-UNA compared to others Animal Facilities of the region. This study standardized the biochemical profile of conventional rats and mice from the Animal Facility of the Facultad de Ciencias Químicas of UNA, offering a valuable tool to researchers for the analysis of their experimental results.

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Scientific Electronic Library Online Paraguay - Paraguay

2020

Nakamura, M. | Miyamoto, T. | Camer, G.A. | Koyama, T. | Matsui, Y. | Sugiura, T. | Moriyoshi, M. | Nakada, K. | Sawamukai, Y.

Cattle with retained fetal membrane (RFM) interrupt optimal dairy productivity including reproductive performance. In this 28-mo duration experimental study, several clinical tests (observation of morbid clinical signs, rectal temperature, vaginal discharge scoring, and blood inflammatory marker measurements) were completed in 19 cattle with RFM including 8 cattle with normally expelled fetal membrane. Twelve out of 19 cattle showed morbid clinical signs at 2.2 ± 1.3 days postpartum (dpp) and pyrexia at 2.3 ± 1.7 dpp. All cattle with RFM had vaginal discharge scores of 1-3 (4-point scale) that steadily persisted for 2-8 wk postpartum. Lower conception rates were noted in cattle with RFM than in cattle with no RFM (64% vs. 100%). Blood haptoglobin (Hp) levels of cattle with RFM and with morbid clinical signs were consistently elevated from 1-10 d (all P 0.01). The Hp of test cattle for 3 d correlated with the 'days open' (Rs

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University Library, University of the Philippines at Los Baños - Philippines

2020

Pulotov, F.Kh. | Devrishov, D.A., The K.I. Skryabin Moscow State Academy of Veterinary Medicine and Biotechnology

Polyvalent vaccines are widely used in the fight against clostridiosis, for the manufacture of which a sufficient amount of bacterial mass and toxins of clostridia are necessary. To obtain the latter, it is advisable to use nutrient media adapted for deep cultivation in an industrial environment. The aim of the work was to develop a prescription composition of nutrient media and improve the industrial technology for the production of polyvalent vaccine against animal clostridiosis. The study included the manufacture of an experimental vaccine series, the selection of the optimal ratio of components and the effective dose of the strains. Conducted chain cultivation of vaccine strains of clostridia in flasks, bottles and bioreactors on a newly developed nutrient medium (the formulation (g/l): liver extract – 10; casein peptone – 22.5; K2NРO4 – 2.3; KH2РO4 – 1.7; MgSO4 – 0.6. This allowed achieving a high yield of bacterial mass and toxin formation with a concentration of microbial cultures of an average of 9.8-11.0 x 10000000000 cells/cm3. In experiments on laboratory animals, it was found that the experimental series of a multivalent vaccine against animal clostridiоsis is harmless, areactogenic, provides the production of humoral immunity with a pronounced antitoxic effect against all strains of clostridia that are part of the drug. The proposed improvement is included in the regulation for the production of a multivalent vaccine against animal clostridiosis at Agrovet LLC. | Summaries (En, Ru) | Широкое применение в борьбе с клостридиозами нашли поливалентные вакцины, для изготовления которых необходимы достаточное количество бактериальной массы и токсина клостридий. Для получения последних целесообразно использовать питательные среды, адаптированные для глубинного культивирования в промышленных условиях. Целью работы было разработать рецептурный состав питательных сред и усовершенствовать промышленную технологию производства поливалентной вакцины против клостридиозов животных. Исследование включало в себя изготовление экспериментальной серии вакцины, подбор оптимального соотношения компонентов и эффективной дозы штаммов. Проводили цепочечное культивирование вакцинных штаммов клостридий во флаконах, бутылях и биореакторах на вновь разработанной питательной среде (состав (г/л): ливерный экстракт – 10; казеин-пептон – 22,5; К2НРО4 – 2,3; КН2РО4 – 1,7; MgSO4 – 0,6). Это позволило достичь высокого выхода бактериальной массы и токсинообразования с концентрацией микробных культур в среднем 9,8-11,0x10E9 кл./см3. В опытах на лабораторных животных установлено, что экспериментальная серия поливалентной вакцины против клостридизов животных является безвредной, ареактогенной, обеспечивает выработку гуморального иммунитета с выраженным антитоксическим действием против всех штаммов клостридий, входящих в состав препарата. Предложенное усовершенствование внесено в регламент производства поливалентной вакцины против клостридиозов животных в ООО "Агровет". | 3 tables | 18 ref.

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Central Scientific Agricultural Library - Russian Federation

2020

Valiullin, L.R. | Mukhammadiev, Rish.S. | Mukhammadiev, Rin.S. | Skvortsov, E.V. | Raginov, I.S., Federal Centre of Toxicologic and Irradiation Safety of Animals, Kazan

Исследования проводили с целью изучения эффективности использования микроорганизмов Bacillus subtilis, Lactococcus lactis, Lactobacillus plantarum для уменьшения токсичности растительного сырья, контаминированного в течении 20 суток микроскопическими грибами Fusarium sporotrichioides и Fusarium oxysporum. Исследования проводили методом биотестирования на простейших, кроликах (кожная проба), мышах, а также в опыте на белых крысах. Эффективность воздействия выбранных штаммов микроорганизмов при длительном (в течение 24 сут) влиянии на организм белых крыс оценивали по изменению живой массы, клинико-гематологических и биохимических показателей крови. При употреблении животными токсичного корма их живая масса к концу эксперимента была меньше, чем в контроле, на 12,1%. В группах, где совместно с токсичным кормом использовали микроорганизмы величина этого показателя изменялась незначительно. У крыс, получавших токсичный корм, концентрация эритроцитов, лейкоцитов и гемоглобина в крови снизилась, в сравнении с контролем, соответственно на 18,2, 25,6 и 15,0%. В группах, где для детоксикации корма использовали микроорганизмы, особенно в сочетании Bacillus subtilis + Lactococcus lactis + Lactobacillus plantarum, гематологические показатели оставались на уровне контроля. В группе с использованием токсичного корма величины АСТ, АЛТ, щелочной фосфотазы и общего билирубина в крови животных были выше, чем в контроле, соответственно на 72,1, 51,2, 44,1, 32,4%, а уровень глюкозы – ниже на 38,7%. При добавлении в токсичный корм микроорганизмов, особенно их консорциума, в состав которого входили штаммы всех трех изучаемых видов, биохимические показатели крови отличались от контроля незначительно. | The research aimed to study the effectiveness of using microorganisms Bacillus subtilis, Lactococcus lactis, Lactobacillus plantarum, and Lactococcus lactis to reduce the toxicity of plant materials contaminated for 14 days by microscopic fungi Fusarium sporotrichioides and Fusarium oxysporum. We conducted bio testing of protozoa, rabbits (skin test), and mice; we also experimented on white rats. The effectiveness of the long-term (for 21 days) effect of the selected strains of microorganisms on the body of white rats was assessed by the change in live weight, as well as by the change in clinical, haematological, and biochemical blood parameters. The live weight of the animals from the experimental group who consumed toxic feed by the end of the experiment was less than the live weight of the animals from the control group by 12.1%. In the groups where microorganisms were used together with toxic food, the value of this indicator changed insignificantly. The concentrations of erythrocytes, leukocytes, and haemoglobin in the blood of the rats fed with toxic food decreased, in comparison with the control by 18.2%, 25.6%, and 15.0%, respectively. In the groups where microorganisms were used to detoxify feed, especially in the combination Bacillus subtilis + Lactococcus lactis + Lactobacillus plantarum, haematological parameters remained at the control level. In the group using toxic food, the values of AST, ALT, alkaline phosphatase, and total bilirubin in the blood of animals were higher than in the control by 72.1%, 51.2%, 44.1%, and 32.4%, respectively; the glucose level was lower by 38.7%. When microorganisms, especially their consortium, which included the strains of all three studied species, were added to the toxic feed the biochemical parameters of the blood did not differ significantly from the control.

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Central Scientific Agricultural Library - Russian Federation

2019

Bogdanova, E.V. | Mel'nikova, E.I. | Grebenshchikov, A.V., Voronezh State Univ. of Engineering Technologies

A technology of reducing allergenicity of the basic whey protein beta-lactoglobulin present in an ultrafiltration concentrate of cheese whey has been developed. Bioconversion o is carried out by using enzyme preparations Flavorpro 750MDP and Promod 439L. The residual antigenicity of the obtained hydrolysate (BLH) is not more than 10%. There has been assessed bioavailability of hydrolysate and its permissible amount in the diet has been determined, which provides necessary level of essential amino acids to maintain vital functions of the body. Experiments were carried out on white inbred BALB/c mice aged of 15 days at onset of the experiment for 28 days. Animals of the control group (n=30) were on a diet consisting of a mixture of cereals with a single source of protein. The BLH was added to the diet of experimental group (n=30). Mass fraction of the total protein did not exceed 10% of dry matter in both diets. The mice were weighted and measured daily. Biochemical analysis of blood composition of experimental animals was carried out before the experiment and after it. According to research results, high bioavailability of the diet with BLH and effective absorption of amino acids in the animal organism during consuming BLH , and absence of load on the organs and tissues of mice have been established. | Разработана технология снижения аллергенности основного сывороточного белка бета-лактоглобулина, присутствующего в ультрафильтрационном концентрате подсырной сыворотки. Биоконверсия проводится с помощью ферментных препаратов Flavorpro 750MDP и Promod 439L. Остаточная антигенность полученного гидролизата (ГБЛ) не превышает 10%. Проведены оценка биодоступности ГБЛ и определение его допустимого количества в рационе питания, обеспечивающего уровень поступления эссенциальных аминокислот, необходимый для поддержания жизнедеятельности организма. Исследования проводили на белых инбредных мышах линии BALB/с (возраст на момент начала опыта 15 дн.) в течение 28 сут. Животных контрольной группы (n=30) содержали на рационе, состоявшем из смеси зерновых с единственным источником белка. В рацион экспериментальной группы (n=30) дополнительно вводили ГБЛ. Массовая доля общего белка в обоих рационах не превышала 10% по сухому веществу. Ежедневно определяли массу мышей и ее суточный прирост. До и после эксперимента проводили биохимический анализ крови мышей. По результатам исследований установлены высокая биодоступность рациона с ГБЛ, эффективное усвоение аминокислот в организме мышей, отсутствие нагрузки на органы и ткани мышей при потреблении ГБЛ в пищу.

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Central Scientific Agricultural Library - Russian Federation

2018

Cardona, H. de | Parra López, L.G.

Bogotá (Colombia) : Instituto Zooprofiláctico Colombiano, 1967 | 19312 | p. 90-96 | Instituto Zooprofiláctico Colombiano, Bogotá (Colombia) | Trabajos presentados. 6. Congreso Nacional de Medicina Veterinaria y Zootecnia. Bogotá (Colombia), 28-31 Ago 1967

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Corporación colombiana de investigación agropecuaria - Colombia

2018

Villafañe Arévalo, F. | Torrenegra G, R.D. | Pedraza Rodríguez, C.E.

Se hizo un estudio experimental con el objeto de relacionar algunas plantas con la etiología de la hematuria vesical bovina. 4 especies vegetales recolectadas y seleccionadas en una región donde existe un alto índice de hematuria fueron suministradas a ratones (Mus musculus c. swiss albino). Las especies Hydrocotyle bomnplandii, Thelipteris rudis y Thuidium peruvianum se proporcionaron a los ratones en forma de extractos acuosos, la planta Chupea racemosa, se suministró en mezcla con el concentrado. La presencia de estas 4 especies, ha sido confirmada en zonas donde se desarrolla la enfermedad. Los resultados histopatológicos, permitieron verificar el desarrollo de micro hematuria en cada uno de los grupos a los que se suministró la especie vegetal. Se efectuó el estudio fitoquímico de la especie H. bonplandii, de la cual se aislaron sustancias con propiedades químicas que pueden tener relación con la etiología de la hematuria vesical bovina | Revista ACOVEZ (Colombia) | 13054 | v. 7 (24) p. 11-19

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Corporación colombiana de investigación agropecuaria - Colombia

2018

Nam, M.H., Seoul National University, Seoul, Republic of Korea | Chun, M.S., Seoul National University, Seoul, Republic of Korea | Seong, J.K., Seoul National University, Seoul, Republic of Korea | Kim, H.G., Hongik University, Seoul, Republic of Korea

The aim of this study is to evaluate the reporting quality of animal experiments in Korea using the Animals in Research: Reporting In Vivo Experiments (ARRIVE) guideline developed in 2010 to overcome the reproducibility problem and to encourage compliance with replacement, refinement and reduction of animals in research (3R's principle). We reviewed 50 papers published by a Korean research group from 2013 to 2016 and scored the conformity with the 20-items ARRIVE guideline. The median conformity score was 39.50%. For more precise evaluation, the 20 items were subdivided into 57 sub-items. Among the sub-items, status of experimental animals, housing and husbandry were described under the average level. Microenvironment sub-items, such as enrichment, bedding material, cage type, number of companions, scored under 10%. Although statistical methods used for the studies were given in most publications (84%), sample size calculation and statistical assumption were rarely described. Most publications mentioned the IACUC approval, but only 8% mentioned welfare-related assessments and interventions, and only 4% mentioned any implications of experimental methods or findings for 3R. We may recommend the revision of the present IACUC proposal to collect more detailed information and improving educational program for animal researchers according to the ARRIVE guideline.

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Korea Agricultural Science Digital Library - Republic of Korea

2018

Yun, J.Y., Chungbuk National University, Cheongju, Republic of Korea | Hyun, B.H., Osong Medical Innovation Foundation, Cheongju, Republic of Korea | Nam, S.Y., Chungbuk National University, Cheongju, Republic of Korea | Yun, Y.W., Chungbuk National University, Cheongju, Republic of Korea | Lee, H.J., Gyeongsang National University, Jinju, Republic of Korea | Lee, B.J., Chungbuk National University, Cheongju, Republic of Korea

This study investigated the anti-cancer potential of a near-infrared fluorescence (NIRF) molecule conjugated with Cetuximab (Cetuximab-NIRF) in six-week-old female BALB/c athymic (nu+/nu+) nude mice. A431 cells were cultured and injected into the animals to induce solid tumors. Paclitaxel (30 mg/kg body weight (BW)), Cetuximab (1 mg/kg BW), and Cetuximab-NIRF (0.25, 0.5 and 1.0 mg/kg BW) were intraperitoneally injected twice a week into the A431 cell xenografts of the nude mice. Changes in BW, tumor volume and weight, fat and lean mass, and diameter of the peri-tumoral blood vessel were determined after two weeks. Tumor volumes and weights were significantly decreased in the CetuximabNIRF (1 mg/kg BW) group compared with the control group (P less than 0.001). Lean mass and total body water content were also conspicuously reduced in the Cetuximab-NIRF (1 mg/kg BW) group compared with the vehicle control group. Peri-tumoral blood vessel diameters were very thin in the Cetuximab-NIRF groups compared with those of the paclitaxel group. These results indicate that the conjugation of Cetuximab with NIRF does not affect the anti-cancer potential of Cetuximab and NIRF can be used for molecular imaging in cancer treatments.

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Korea Agricultural Science Digital Library - Republic of Korea

2018

Lee, S., Pusan National University, Busan, Republic of Korea | Kwak, J.H., Kyungsung University, Busan, Republic of Korea | Kim, S.H., Pusan National University, Busan, Republic of Korea | Yun, J., Cheongju University, Cheongju, Republic of Korea | Cho, J.Y., Korea National Sport University, Seoul, Republic of Korea | Kim, K., Kyungpook National University, Daegu, Republic of Korea | Hwang, D., Pusan National University, Miryang, Republic of Korea | Jung, Y.S., Pusan National University, Busan, Republic of Korea

Animal models have been used to elucidate the pathophysiology of varying diseases and to provide insight into potential targets for therapeutic intervention. Although alternatives to animal testing have been proposed to help overcome potential drawbacks related to animal experiments and avoid ethical issues, their use remains vital for the testing of new drug candidates and to identify the most effective strategies for therapeutic intervention. Particularly, the study of metabolic diseases requires the use of animal models to monitor whole-body physiology. In line with this, the National Institute of Food and Drug Safety Evaluation (NIFDS) in Korea has established their own animal strains to help evaluate both efficacy and safety during new drug development. The objective of this study was to characterize the response of C57BL/6NKorl mice from the NIFDS compared with that of other mice originating from the USA and Japan in a chemical-induced diabetic condition. Multiple low-dose treatments with streptozotocin were used to generate a type-1 diabetic animal model which is closely linked to the known clinical pathology of this disease. There were no significantly different responses observed between the varying streptozotocin-induced type-1 diabetic models tested in this study. When comparing control and diabetic mice, increases in liver weight and disturbances in serum amino acids levels of diabetic mice were most remarkable. Although the relationship between type-1 diabetes and BCAA has not been elucidated in this study, the results, which reveal a characteristic increase in diabetic mice of all origins are considered worthy of further study.

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Korea Agricultural Science Digital Library - Republic of Korea