Genetics

**Genetics** is a discipline of biology. It is the science of heredity. This includes the study of genes, and the inheritance of variation and traits of living organisms. In the laboratory, genetics works by mating carefully selected organisms, and analysing their offspring. More informally, genetics is the study of how parents pass some of their characteristics to their children. It is an important part of biology, and gives the basic rules on which evolution acts.

The fact that living things inherit traits from their parents has been known since prehistoric times. It is used to improve crop plants and animals by selective breeding. The modern science of genetics tries to understand the process of inheritance. This began with the work of Gregor Mendel in the mid-nineteenth century. Although he did not know the physical basis for heredity, Mendel saw that organisms inherit traits via discrete units of inheritance, now called genes.

Modern genetics has expanded beyond inheritance. It studies the way genes work.

## DNA 
Living things are made of millions of tiny self-contained components called cells. Inside each cell are long and complex molecules called deoxyribonucleic acid, known for short as DNA. Some DNA stores information for making proteins. The bits of DNA which do this are known as genes. People look different from each other mainly because they have different versions of the human set of genes. 

However, a large part of DNA (more than 98% for humans) is non-coding DNA. These sections do not serve as patterns for protein sequences. What it does is code for important non-protein information. Examples are various important RNA molecules, and "scaffolding" bits and pieces like centromeres and telomeres. 

Every cell in the same living thing has the same DNA, but only some of it is used in each cell. For instance, some genes that tell how to make parts of the liver are switched off in the brain. What genes are used can also change over time. For instance, a lot of genes are used by a child early in pregnancy that are not used later.

A person has two copies of each gene, one from their mother, and one from their father. There can be several types of a single gene, which give different instructions: one version might cause a person to have blue eyes, another might cause them to have brown. These different versions are known as *alleles* of the gene.

Since a living thing has two copies of each gene, it can have two different alleles of it at the same time. Often, one allele will be *dominant*, meaning that the living thing looks and acts as if it had only that one allele. The unexpressed allele is called *recessive*. In other cases, you end up with something in between the two possibilities. Both alleles are expressed.

Most of the characteristics that you can see in a living thing have more than one gene which influences them. And many genes have multiple effects on the body, because their function will not have the same effect in each tissue. The multiple effects of a single gene is called *pleiotropism*. The whole set of genes is called the *genotype*, and the total effect of genes on the body is called the *phenotype*. These are key terms in genetics.

Imre Festetics, who published work in German in the first part of the 19th century, was totally forgotten until recently. He described several rules of genetic inheritance in his work  *Die genetische Gesätze der Natur*, 1819 (The genetic law of nature). His second law is the same as Mendel. In his third law, he developed the basic principles of mutation. None of the histories of genetics published in the 20th century mentions him.

Probably the most publicized idea before Mendel was that of Charles Darwin, whose idea of *pangenesis* had two parts. The first, that persistent hereditary units were passed on from one generation to another, was quite right. The second was his idea that they were replenished by 'gemmules' from the somatic (body) tissues. This was entirely wrong, and plays no part in science today. Darwin was right about one thing: whatever happens in evolution must happen by means of heredity, and so an accurate science of genetics is fundamental to the theory of evolution. This 'mating' between genetics and evolution took many years to organise. It resulted in the modern evolutionary synthesis.

### Mendelian genetics 
The basic rules of genetics were discovered by Imre Festetics, a landowner (17641847), and a monk named Gregor Mendel around 1865. For thousands of years people had noticed how some traits in parents are passed to their children. However, Mendel's work was different because he designed his experiments very carefully.

In his experiments, Mendel studied how traits were passed on in pea plants. He started his crosses with plants that bred true, and counted characters that were either/or in nature (either *tall* or *short*). He bred large numbers of plants, and expressed his results numerically. He used test crosses to reveal the presence and proportion of recessive characters.

Mendel explained the results of his experiment using two scientific laws:
* **1**. Factors, later called genes, normally occur in pairs in ordinary body cells, yet separate during the formation of sex cells. These factors determine the organism's traits, and are inherited from its parents. When gametes are produced by meiosis, the two factors separate. A gamete only receives one or the other. This Mendel called the *Law of segregation*.
* **2**. Alleles of different genes separate independently of one another when gametes are formed. This he called the *Law of Independent Assortment*. So Mendel thought that different traits are inherited independently of one another. We now know this is only true if the genes are not on the same chromosome, in which case they are not linked to each other.

Mendel's laws helped explain the results he observed in his pea plants. Later, geneticists discovered that his laws were also true for other living things, even humans. Mendel's findings from his work on the garden pea plants helped to establish the field of genetics. His contributions were not limited to the basic rules that he discovered. Mendel's care towards controlling experiment conditions along with his attention to his numerical results set a standard for future experiments. Over the years, scientists have changed and improved Mendel's ideas. However, the science of genetics would not be possible today without the early work of Gregor Mendel.

### Between Mendel and modern genetics 
Between Mendel's work and 1900 the foundations of cytology, the study of cells, was developed. The facts discovered about the nucleus and cell division were essential for Mendel's work to be properly understood.

:1832: Barthélémy Dumortier, the first to observe cell division in a multicellular organism.
:1841, 1852: Robert Remak  (1815–1865), a Jewish Polish–German physiologist, was the first person to state the foundation of cell biology: that cells only derive from other cells. This was later popularized by the German doctor Rudolf Virchow (1821–1902), who used the famous phrase *omnis cellula e cellula*, meaning, all cells from other cells.
:1865: Gregor Mendel's paper, *Experiments on plant hybridization* was published.
:1876: Meiosis was discovered and described for the first time in sea urchin eggs, by German biologist Oscar Hertwig (1849–1922).
:1878–1888: Walther Flemming and Eduard Strasburger describe chromosome behaviour during mitosis.
:1883: Meiosis was described at the level of chromosomes, by Belgian zoologist Edouard van Beneden (1846–1910), in *Ascaris* (roundworm) eggs.
:1883: German zoologist Wilhelm Roux (1850–1924) realised the significance of the linear structure of chromosomes. Their splitting into two equal longitudinal halves meant each daughter cell got the same chromosome complement. Therefore, chromosomes were the bearers of heredity.
:1889: Dutch botanist Hugo de Vries suggests that "inheritance of specific traits in organisms comes in particles", naming such particles *(pan)genes*.
:1890: The significance of meiosis for reproduction and inheritance was described in 1890 by German biologist August Weismann (1834–1914). He noted that two cell divisions were necessary to turn one diploid cell into four haploid cells if the number of chromosomes was to be maintained.
:1902–1904: Theodor Boveri (1862–1915), a German biologist, in a series of papers, drew attention to the correspondence between the behaviour of chromosomes and the results obtained by Mendel. He said that chromosomes were "independent entities which keep their independence even in the resting nucleus... What comes out of the nucleus is what goes into it".
:1903: Walter Sutton suggested that chromosomes, which segregate in a Mendelian fashion, are hereditary units. Edmund B. Wilson (1856–1939), Sutton's teacher, was the author of one of the most famous text-books in biology. Wilson called this idea the *Sutton–Boveri hypothesis*.

At this point, discoveries in cytology merged with the rediscovered ideas of Mendel to make a fusion called **cytogenetics**, (cyto = cell; genetics = heredity) which has continued to the present day.

### Rediscovery of Mendel's work 
During the 1890s several biologists began doing experiments on breeding. and soon Mendel's results were duplicated, even before his papers were read. Carl Correns and Hugo de Vries were the main rediscoverers of Mendel's writings and laws. Both acknowledged Mendel's priority, although it is probable that de Vries did not understand his own results until after reading Mendel. Though Erich von Tschermak was originally also credited with rediscovery, this is no longer accepted because he did not understand Mendel's laws. Though de Vries later lost interest in Mendelism, other biologists built genetics into a science.

Mendel's experimental results were later the object of some debate. Fisher analysed the results of the F2 (second filial) ratio and found them to be implausibly close to the exact ratio of 3 to 1. It is sometimes suggested that Mendel may have censored his results, and that his seven traits each occur on a separate chromosome pair, an extremely unlikely occurrence if they were chosen at random. In fact, the genes Mendel studied occurred in only four linkage groups, and only one gene pair (out of 21 possible) is close enough to show deviation from independent assortment; this is not a pair that Mendel studied.

## Tools of genetics 
### Mutations 
During the process of DNA replication, errors sometimes occur. These errors, called mutations, can have an effect on the phenotype of an organism. In turn, that usually has an effect on the organism's fitness, its ability to live and reproduce successfully.

Error rates are usually very low—1 error in every 10–100&nbsp;million bases—due to the "proofreading" ability of DNA polymerases. Error rates are a thousandfold higher in many viruses. Because they rely on DNA and RNA polymerases which lack proofreading ability, they get higher mutation rates.

Processes that increase the rate of changes in DNA are called mutagenic. Mutagenic chemicals increase errors in DNA replication, often by interfering with the structure of base-pairing, while UV radiation induces mutations by causing damage to the DNA structure. Twin studies of the modern kind did not appear until the 1920s.

## Genetics of prokaryotes and viruses 
The genetics of bacteria, archaea and viruses is a major field of research. Bacteria mostly divide by asexual cell division, but do have a kind of sex by horizontal gene transfer. Bacterial conjugation, transduction and transformation are their methods. In addition, the complete DNA sequence of many bacteria, archaea and viruses is now known.

Although many bacteria were given generic and specific names, like *Staphylococcus aureus*, the whole idea of a species is rather meaningless for an organism which does not have sexes and crossing-over of chromosomes. Instead, these organisms have **strains**, and that is how they are identified in the laboratory.

## Genes and development 
### Gene expression 
Gene expression is how the heritable information in a gene, the sequence of DNA base pairs, is made into a functional gene product, such as protein or RNA. The basic idea is that DNA is transcribed into RNA, which is then translated into proteins. Proteins make many of the structures and all the enzymes in a cell or organism.

Several steps in the gene expression process may be modulated (tuned). This includes both the transcription and translation stages, and the final folded state of a protein. Gene regulation switches genes on and off, and so controls cell differentiation, and morphogenesis. Gene regulation may also serve as a basis for evolutionary change: control of the timing, location, and amount of gene expression can have a profound effect on the development of the organism. The expression of a gene may vary a lot in different tissues. This is called pleiotropism, a widespread phenomenon in genetics.

Alternative splicing is a modern discovery of great importance. It is a process where *from a single gene a large number of variant proteins can be assembled*. One particular *Drosophila* gene (DSCAM) can be alternatively spliced into 38,000 different mRNA molecules.

### Epigenetics & control of development 
Epigenetics is the study of changes in gene activity which are *not* caused by changes in the DNA sequence. It is the study of gene expression, the way genes bring about their phenotypic effects.

These changes in gene activity may stay for the remainder of the cell's life and may also last for many generations of cells, through cell divisions. However, there is no change in the underlying DNA sequence of the organism. Instead, non-hereditary factors cause the organism's genes to behave (express themselves) differently.

Hox genes are a complex of genes whose proteins bind to the regulatory regions of target genes. The target genes then activate or repress cell processes to direct the final development of the organism.

### Extranuclear inheritance 
There are some kinds of heredity which happen outside the cell nucleus. Normal inheritance is from both parents via the chromosomes in the nucleus of a fertilised egg cell. There are some kinds of inheritance other than this.

#### Organelle heredity 
Mitochondria and chloroplasts carry some DNA of their own. Their make-up is decided by genes in the chromosomes and genes in the organelle. Carl Correns discovered an example in 1908. The four o'clock plant, *Mirabilis jalapa*, has leaves which may be white, green or variegated. Correns discovered the pollen had no influence on this inheritance. The colour is decided by genes in the chloroplasts.

#### Infectious heredity 
This is caused by a symbiotic or parasitic relationship with a microorganism.

#### Maternal effect 
In this case nuclear genes in the female gamete are transcribed. The products accumulate in the egg cytoplasm, and have an effect on the early development of the fertilised egg. The coiling of a snail, *Limnaea peregra*, is determined like this. Right-handed shells are genotypes *Dd* or *dd*, while left-handed shells are *dd*.

The most important example of maternal effect is in *Drosophila melanogaster*. The protein product maternal-effect genes activate other genes, which in turn activate still more genes. This work won the Nobel Prize in Physiology or Medicine for 1995. The two scientists shared the 2020 Nobel Prize in Chemistry.

### Genetics of human behaviour 
Many well-known disorders of human behaviour have a genetic component. This means that their inheritance partly causes the behaviour, or makes it more likely the problem would occur. Examples include:
*Autism
*ADHD (attention deficit disorder)
*Drug use and abuse
*Risk taking
*Schizophrenia

Also, normal behaviour is also heavily influenced by heredity:
*Learning and cognitive ability
*Personality.

## Related pages 
*ENCODE: the complete analysis of the human genome
*Gene therapy
*Epigenetics
*Variation

## References 
### Standard works 
*Alberts B, Bray D, Hopkin K, Johnson A, Lewis J, Raff M, Roberts K, Walter P. 2013. *Essential cell biology*, 4th Edition. Garland Science. ISBN 978-1-317-80627-1.
*Griffiths A.J.H. & others 2000. *An introduction to genetic analysis*. 7th ed, Freeman, New York.  https://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=iga.section.60
*Hartl D. & Jones E. 2005. *Genetics: analysis of genes and genomes*. 6th ed, Jones & Bartlett. .
*King R.C;  Mulligan P.K. & Stansfield W.D. 2013. *A dictionary of genetics*. 8th ed, Oxford University Press. 
*Klug, William S. *et al* 2012. *Concepts of genetics*. 10th ed, Pearson. .
*Lodish H, Berk A, Zipursky LS, Matsudaira P, Baltimore D, Darnell J(2000. Molecular Cell Biology. 4th ed, New York: Scientific American Books. ISBN 978-0-7167-3136-8

#### Works on genetic manipulation 
*Jennifer A. Doudna and Samuel H. Sternberg. 2017. *A Crack in Creation: gene editing and the unthinkable power to control evolution*. Houghton Mifflin Harcourt. ISBN 978-1-847-92382-0
*Carey, Nessa 2011. *The epigenetics revolution: how modern biology is rewriting our understanding of genetics, disease and inheritance*. London: Icon Books. ISBN 978-184831347-1
*Carey, Nessa 2019. *Hacking the code of life: how gene editing will write our futures*. London: Icon Books. ISBN 978-1-78578-625-9