ជញ្ជាំងកោសិកា

A **cell wall** is a structural layer surrounding some types of cells, situated outside the cell membrane. It can be tough, flexible, and sometimes rigid. It provides the cell with both structural support and protection, and also acts as a filtering mechanism. Cell walls are present in most prokaryotes (except mycoplasma bacteria), in algae, plants and fungi but rarely in other eukaryotes including animals.  A major function is to act as pressure vessels, preventing over-expansion of the cell when water enters.

The composition of cell walls varies between species and may depend on cell type and developmental stage. The primary cell wall of land plants is composed of the polysaccharides cellulose, hemicellulose and pectin. Often, other polymers such as lignin, suberin or cutin are anchored to or embedded in plant cell walls. Algae possess cell walls made of glycoproteins and polysaccharides such as carrageenan and agar that are absent from land plants. In bacteria, the cell wall is composed of peptidoglycan. The cell walls of archaea have various compositions, and may be formed of glycoprotein S-layers, pseudopeptidoglycan, or polysaccharides. Fungi possess cell walls made of the glucosamine polymer chitin. Unusually, diatoms have a cell wall composed of biogenic silica.

## ប្រវត្តិសាស្ត្រ
A plant cell wall was first observed and named (simply as a "wall") by Robert Hooke in 1665. However, "the dead excrusion product of the living protoplast" was forgotten, for almost three centuries, being the subject of scientific interest mainly as a resource for industrial processing or in relation to animal or human health.

In 1804, Karl Rudolphi and J.H.F. Link proved that cells had independent cell walls. Before, it had been thought that cells shared walls and that fluid passed between them this way.

The mode of formation of the cell wall was controversial in the 19th century. Hugo von Mohl (1853, 1858) advocated the idea that the cell wall grows by apposition. Carl Nägeli (1858, 1862, 1863) believed that the growth of the wall in thickness and in area was due to a process termed intussusception. Each theory was improved in the following decades: the apposition (or lamination) theory by Eduard Strasburger (1882, 1889), and the intussusception theory by Julius Wiesner (1886).

In 1930, Ernst Münch coined the term *apoplast* in order to separate the "living" symplast from the "dead" plant region, the latter of which included the cell wall.

By the 1980s, some authors suggested replacing the term "cell wall", particularly as it was used for plants, with the more precise term "extracellular matrix", as used for animal cells, but others preferred the older term.

## Properties
Cell walls serve similar purposes in those organisms that possess them. They may give cells rigidity and strength, offering protection against mechanical stress. In multicellular organisms, they permit the organism to build and hold a definite shape (morphogenesis). Cell walls also limit the entry of large molecules that may be toxic to the cell. They further permit the creation of stable osmotic environments by preventing osmotic lysis and helping to retain water. Their composition, properties, and form may change during the cell cycle and depend on growth conditions.

### Rigidity of cell walls
In most cells, the cell wall is flexible, meaning that it will bend rather than holding a fixed shape, but has considerable tensile strength. The apparent rigidity of primary plant tissues is enabled by cell walls, but is not due to the walls' stiffness. Hydraulic turgor pressure creates this rigidity, along with the wall structure. The flexibility of the cell walls is seen when plants wilt, so that the stems and leaves begin to droop, or in seaweeds that bend in water currents. As John Howland explains:

The apparent rigidity of the cell wall thus results from inflation of the cell contained within. This inflation is a result of the passive uptake of water.

In plants, a **secondary cell wall** is a thicker additional layer of cellulose which increases wall rigidity. Additional layers may be formed by lignin in xylem cell walls, or suberin in cork cell walls. These compounds are rigid and waterproof, making the secondary wall stiff. Both wood and bark cells of trees have secondary walls. Other parts of plants such as the leaf stalk may acquire similar reinforcement to resist the strain of physical forces.

### Permeability
The primary cell wall of most plant cells is freely permeable to small molecules including small proteins, with size exclusion estimated to be 30-60 kDa.  The pH is an important factor governing the transport of molecules through cell walls.

## ការវិវត្តន៍
Cell walls evolved independently in many groups, even in the photosynthetic eukaryotes. In these lineages, the cell wall is closely related to the evolution of multicellularity, terrestrialization and vascularization.

## Plant cell walls
The walls of plant cells must have sufficient tensile strength to withstand internal osmotic pressures of several times atmospheric pressure that result from the difference in solute concentration between the cell interior and external solutions. Plant cell walls vary from 0.1 to several µm in thickness.

### ស្រទាប់
Up to three strata or layers may be found in plant cell walls:

*The **primary cell wall**, generally a thin, flexible and extensible layer formed while the cell is growing.
*The **secondary cell wall**, a thick layer formed inside the primary cell wall after the cell is fully grown. It is not found in all cell types. Some cells, such as the conducting cells in xylem, possess a secondary wall containing lignin, which strengthens and waterproofs the wall.
*The **middle lamella**, a layer rich in pectins. This outermost layer forms the interface between adjacent plant cells and glues them together.

### ការ​តែង​និពន្ធ
In the primary (growing) plant cell wall, the major carbohydrates are cellulose, hemicellulose and pectin. The cellulose microfibrils are linked via hemicellulosic tethers to form the cellulose-hemicellulose network, which is embedded in the pectin matrix. The most common hemicellulose  in the primary cell wall is xyloglucan. In grass cell walls, xyloglucan and pectin are reduced in abundance and partially replaced by glucuronarabinoxylan, another type of hemicellulose. Primary cell walls characteristically extend (grow) by a mechanism called acid growth, which involves turgor-driven movement of the strong cellulose microfibrils within the weaker hemicellulose/pectin matrix, catalyzed by expansin proteins. The outer part of the primary cell wall of the plant epidermis is usually impregnated with cutin and wax, forming a permeability barrier known as the plant cuticle.

Secondary cell walls contain a wide range of additional compounds that modify their mechanical properties and permeability. The major polymers that make up wood (largely secondary cell walls) include:
* cellulose, 35-50%
* xylan, 20-35%, a type of hemicellulose
* lignin, 10-25%, a complex phenolic polymer that penetrates the spaces in the cell wall between cellulose, hemicellulose and pectin components, driving out water and strengthening the wall.

Additionally, structural proteins (1-5%) are found in most plant cell walls; they are classified as hydroxyproline-rich glycoproteins (HRGP), arabinogalactan proteins (AGP), glycine-rich proteins (GRPs), and proline-rich proteins (PRPs). Each class of glycoprotein is defined by a characteristic, highly repetitive protein sequence. Most are glycosylated, contain hydroxyproline (Hyp) and become cross-linked in the cell wall. These proteins are often concentrated in specialized cells and in cell corners. Cell walls of the epidermis may contain cutin. The Casparian strip in the endodermis roots and cork cells of plant bark contain suberin. Both cutin and suberin are polyesters that function as permeability barriers to the movement of water. The relative composition of carbohydrates, secondary compounds and proteins varies between plants and between the cell type and age. Plant cells walls also contain numerous enzymes, such as hydrolases, esterases, peroxidases, and transglycosylases, that cut, trim and cross-link wall polymers.

Secondary walls - especially in grasses - may also contain microscopic silica crystals, which may strengthen the wall and protect it from herbivores.

Cell walls in some plant tissues also function as storage deposits for carbohydrates that can be broken down and resorbed to supply the metabolic and growth needs of the plant. For example, endosperm cell walls in the seeds of cereal grasses, nasturtium
and other species, are rich in glucans and other polysaccharides that are readily digested by enzymes during seed germination to form simple sugars that nourish the growing embryo.

### Formation
The middle lamella is laid down first, formed from the cell plate during cytokinesis, and the primary cell wall is then deposited inside the middle lamella. The actual structure of the cell wall is not clearly defined and several models exist - the covalently linked cross model, the tether model, the diffuse layer model and the stratified layer model. However, the primary cell wall, can be defined as composed of cellulose microfibrils aligned at all angles. Cellulose microfibrils are produced at the plasma membrane by the cellulose synthase complex, which is proposed to be made of a hexameric rosette that contains three cellulose synthase catalytic subunits for each of the six units. Microfibrils are held together by hydrogen bonds to provide a high tensile strength. The cells are held together and share the gelatinous membrane called the *middle lamella*, which contains magnesium and calcium pectates (salts of pectic acid). Cells interact though plasmodesmata, which are inter-connecting channels of cytoplasm that connect to the protoplasts of adjacent cells across the cell wall.

In some plants and cell types, after a maximum size or point in development has been reached, a *secondary wall* is constructed between the plasma membrane and primary wall. Unlike the primary wall, the cellulose microfibrils are aligned parallel in layers, the orientation changing slightly with each additional layer so that the structure becomes helicoidal.  Cells with secondary cell walls can be rigid, as in the gritty sclereid cells in pear and quince fruit. Cell to cell communication is possible through pits in the secondary cell wall that allow plasmodesmata to connect cells through the secondary cell walls.

## Fungal cell walls
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There are several groups of organisms that have been called "fungi". Some of these groups (Oomycete and Myxogastria) have been transferred out of the Kingdom Fungi, in part because of fundamental biochemical differences in the composition of the cell wall.  Most true fungi have a cell wall consisting largely of chitin and other polysaccharides.  True fungi do not have cellulose in their cell walls.

### True fungi
In fungi, the cell wall is the outer-most layer, external to the plasma membrane. The fungal cell wall is a matrix of three main components:
* chitin: polymers consisting mainly of unbranched chains of β-(1,4)-linked-N-Acetylglucosamine in the Ascomycota and Basidiomycota, or poly-β-(1,4)-linked-N-Acetylglucosamine (chitosan) in the Zygomycota. Both chitin and chitosan are synthesized and extruded at the plasma membrane. Algal cell walls contain either polysaccharides (such as cellulose (a glucan)) or a variety of glycoproteins (Volvocales) or both. The inclusion of additional polysaccharides in algal cells walls is used as a feature for algal taxonomy.

* Mannans: They form microfibrils in the cell walls of a number of marine green algae including those from the genera, *Codium*, *Dasycladus*, and *Acetabularia* as well as in the walls of some red algae, like *Porphyra* and *Bangia*.
* Xylans:
* Alginic acid: It is a common polysaccharide in the cell walls of brown algae.
* Sulfonated polysaccharides: They occur in the cell walls of most algae; those common in red algae include agarose, carrageenan, porphyran, furcelleran and funoran.

Other compounds that may accumulate in algal cell walls include sporopollenin and calcium ions.

The group of algae known as the diatoms synthesize their cell walls (also known as frustules or valves) from silicic acid (specifically orthosilicic acid, H4SiO4). The acid is polymerised intra-cellularly, then the wall is extruded to protect the cell. Significantly, relative to the organic cell walls produced by other groups, silica frustules require less energy to synthesize (approximately 8%), potentially a major saving on the overall cell energy budget and possibly an explanation for higher growth rates in diatoms.

In brown algae, phlorotannins may be a constituent of the cell walls.

### Water molds
The group Oomycetes, also known as water molds, are saprotrophic plant pathogens like fungi. Until recently they were widely believed to be fungi, but structural and molecular evidence has led to their reclassification as heterokonts, related to autotrophic brown algae and diatoms.  Unlike fungi, oomycetes typically possess cell walls of cellulose and glucans rather than chitin, although some genera (such as *Achlya* and *Saprolegnia*) do have chitin in their walls.  The fraction of cellulose in the walls is no more than 4 to 20%, far less than the fraction of glucans. The spore wall has been shown to possess three layers, the middle of which is composed primarily of cellulose, and the innermost is sensitive to cellulase and pronase. Bacterial cell walls are different from the cell walls of plants and fungi which are made of cellulose and chitin, respectively. The cell wall of bacteria is also distinct from that of Archaea, which do not contain peptidoglycan. The cell wall is essential to the survival of many bacteria, although L-form bacteria can be produced in the laboratory that lack a cell wall.  The antibiotic penicillin is able to kill bacteria by preventing the cross-linking of peptidoglycan and this causes the cell wall to weaken and lyse.

Gram-positive bacteria possess a thick cell wall containing many layers of peptidoglycan and teichoic acids. In contrast, Gram-negative bacteria have a relatively thin cell wall consisting of a few layers of peptidoglycan surrounded by a second lipid membrane containing lipopolysaccharides and lipoproteins. Most bacteria have the Gram-negative cell wall and only the Firmicutes and Actinobacteria (previously known as the low G+C and high G+C Gram-positive bacteria, respectively) have the alternative Gram-positive arrangement. These differences in structure can produce differences in antibiotic susceptibility, for instance vancomycin can kill only Gram-positive bacteria and is ineffective against Gram-negative pathogens, such as *Haemophilus influenzae* or *Pseudomonas aeruginosa*.

### Archaeal cell walls
Although not truly unique, the cell walls of Archaea are unusual. Whereas peptidoglycan is a standard component of all bacterial cell walls, all archaeal cell walls lack peptidoglycan, with the exception of one group of methanogens.  While the overall structure of archaeal *pseudo*peptidoglycan superficially resembles that of bacterial peptidoglycan, there are a number of significant chemical differences. Like the peptidoglycan found in bacterial cell walls, pseudopeptidoglycan consists of polymer chains of glycan cross-linked by short peptide connections.  However, unlike peptidoglycan, the sugar N-acetylmuramic acid is replaced by N-acetyltalosaminuronic acid, Many algae have a sheath or envelope of mucilage outside the cell made of exopolysaccharides. Diatoms build a frustule from silica extracted from the surrounding water; radiolarians, foraminiferans, testate amoebae and silicoflagellates also produce a skeleton from minerals, called test in some groups.  Many green algae, such as *Halimeda* and the Dasycladales, and some red algae, the Corallinales, encase their cells in a secreted skeleton of calcium carbonate.  In each case, the wall is rigid and essentially inorganic. It is the non-living component of cell. Some golden algae, ciliates and choanoflagellates  produces a shell-like protective outer covering called lorica. Some dinoflagellates have a theca of cellulose plates, and coccolithophorids have coccoliths.

An extracellular matrix is also present in metazoans. Its composition varies between cells, but collagens are the most abundant protein in the ECM.

## សូម​មើល​ផង​ដែរ
* Bacterial cell structure
* Plant cell

## ឯកសារយោង
## តំណ​ភ្ជាប់​ខាងក្រៅ
* Cell wall ultrastructure
* The Cell Wall