Title: Contrast techniques in light microscopy
First published: 1996
Description: Contrast in an image is essential to distinguish features from one another and from the background. Contrast Techniques in Light Microscopy is a practical handbook describing the ways in which light interacts with the specimen in the microscope and the manner in which this is used to generate contrast in the image of the specimen. The book shows ways in which such interactions are used in practice and includes examples and case studies of contrast enhancement. The book describes techniques for both transmitted and reflected light, as well as the effects of the refractive index of the mounting medium surrounding the specimen. Techniques covered include bright field and dark ground, polarized light, phase contrast, modulation contrast, interference and fluorescence, and also contrast manipulation outside the microscope. Contrast Techniques in Light Microscopy is an essential practical guide for all microscopists.
Subjects: Phase-contrast microscopy, Microscopy, technique
LC classification: QH212.P5 B73 1996, QH212.P5 B73 1996