Title: Manipulating the mouse embryo
Description: Provides background information and detailed protocols for developing a mouse colony and using the animals in transgenic and gene-targeting experiments. The protocols list the animals, equipment, and reagents required and step-by-step procedures. Topics include in vitro culture of preimplantation embryos, surgical procedures, the production of chimeras, and the analysis of genome alterations. The third edition adds protocols for cloning mice, modifying embryonic stem cells, intracytoplasmic sperm injection, and cryopreservation of embryos.
Subjects: Mice, Laboratory manuals, Embryos, Genetic engineering, Transgenic mice, Mice as laboratory animals, Embryology, Genetics, Souris, Manuels de laboratoire, Embryons, Génie génétique, Souris transgéniques, Souris (Animal de laboratoire), Embryo, Embryonalentwicklung, Experiment, Maus, Transgene Tiere, Gentechnologie, Labortechnik, Ratos (embriologia), Animais de laboratório, Embriologia comparada, Embriologia, Manuals de laboratori, Transgenic animals