Further investigation of the role of Fli-1 in haematopoiesis using a conditional transgenic mouse model.
Published: 2005
Language: eng
Number of pages: 57
Description: The ets transcription factor Fli-1 is essential for murine vascular development and is important for the development of lymphocytes, erythrocytes, megakaryocytes, granulocytes and monocytes. More importantly, Fli-1 plays roles in various murine and human cancers, warranting further investigation into its natural and pathological functions.A tissue-specific Fli-1 overexpressing transgenic mouse model was generated using the Cre-loxP system, in which exogenous Fli-1 expression can be traced by the green fluorescence gene. Overexpression in haematopoietic and endothelial tissue caused a partial block in the development of EGFP+ double negative (CD8-/CD4-) thymocytes. Furthermore, Fli-1 overexpressing splenic T and B cells showed a marked decrease in proliferation after mitogenic stimuli. This indicates that both T cell development and proliferation are tightly controlled by ETS transcription factors; it is necessary to examine the other members of this family in the EGFP+ and negative cells from these mice, in addition to analyzing other haematopoietic lineages for appropriate markers and function.
Notes: Source: Masters Abstracts International, Volume: 44-02, page: 0779.

Thesis (M.Sc.)--University of Toronto, 2005.

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