RNA
Subtitle: methods and protocols
By: edited by Henrik Nielsen
Series: Springer Protocols; Methods in molecular biology -- 703; Springer protocols (Series); Methods in molecular biology (Clifton, N.J.) -- v. 703.
Publisher: Humana Press
Published: 2011
Place of publication: New York, N.Y
Language: eng
Number of pages: 329
Copyright date: 2011
Subjects: Messenger RNA; Non-coding RNA; Untranslated RNA
Notes: Includes bibliographical references and index.
Table of contents: title: The transcriptional landscape; title: Working with RNA; title: Synthesis of RNA by in vitro transcription; title: Efficient poly(A)+ RNA selection using LNA oligo(T) capture; title: Genome browsers; title: Web-based tools for studying RNA structure and function; title: Northern blotting analysis; title: Rapid amplification of cDNA ends (RACE); title: Fractionation of mRNA based on the length of the poly(A) tail; title: Analysis of mutations that influence pre-mRNA splicing; title: S1 nuclease analysis of alternatively spliced mRNA; title: Promises and challenges in developing RNAi as a research tool and therapy; title: Inhibition of gene function in mammalian cells using short-hairpin RNA (shRNA); title: Validation of RNAi by real time PCR; title: Profiling RNA polymerase II using the fast chromatin immunoprecipitation method; title: The post-transcriptional operon; title: RIP-chip analysis : RNA-binding protein immunoprecipitation-microarray (chip) profiling; title: Isolation of RNP granules; title: Electrophoretic mobility shift assay for characterizing RNA-protein interaction; title: Polysome analysis and RNA purification from sucrose gradients; title: Prediction of targets for microRNAs; title: Outsourcing of experimental work.