Drug-DNA interaction protocols
By: edited by Keith R. Fox
Edition: 2nd ed.
Series: Springer protocols; Methods in molecular biology -- 613; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 613.
Publisher: Humana Press, Springer Science+Business Media
Published: 2010
Place of publication: New York, N.Y
Language: eng
Number of pages: 318
ISBN-13: 9781603274173
Subjects: Pharmaceutical Preparations; DNA; Methods; DNA Footprinting; Drug effects; DNA-drug interactions; DNA Probes; Polymerase Chain Reaction; DNA-Binding Proteins; Chemistry; Laboratory Manuals; Metabolism
Notes: Includes bibliographical references and index.
Table of contents: title: 1. Quantitative analysis of small molecule-nucleic acid interactions with a biosensor surface and surface plasmon resonance detection / Yang Liu and W. David Wilson; title: 2. Thermal melting studies of ligand DNA interactions / Aurore Guédin, Laurent Lacroix, and Jean-Louis Mergny; title: 3. Circular and linear dichroism of drug-DNA systems / Alison Rodger; title: 4. Drug binding to DNA-RNA hybrid structures / Richard T. Wheelhouse and Jonathan B. Chaires; title: 5. Quantification of binding data using capillary electrophoresis / Fitsumbirhan Araya, Graham G. Skellern, and Roger D. Waigh; title: 6. Determination of equilibrium association constants of ligand-DNA complexes by electrospray mass spectrometry / Valérie Gabelica; title: 7. Detection of adriamycin-DNA adducts by accelerator mass spectrometry / Kate Coldwell ... [et al.]; title: 8. Molecular modelling methods to quantitate drug-DNA interactions / Hao Wang and Charles A. Laughton; title: 9. Application of anomalous diffraction methods to the study of DNA and DNA-complexes / Derrick Watkins ... [et al.]; title: 10. DNase I footprinting / Antonia S. Cardew and Keith R. Fox; title: 11. Methods to characterize the effect of DNA-modifying compounds on nucleosomal DNA / Vidya Subramanian ... [et al.]; title: 12. REPSA: combinatorial approach for identifying preferred drug-DNA binding sequences / Michael W. Van Dyke; title: 13. In vitro transcription assay for resolution of drug-DNA interactions at defined DNA sequences / Benny J. Evison, Don. R. Phillips, and Suzanne M. Cutts; title: 14. In virtro footprinting of promoter regions within supercoiled plasmid DNA / Daekyu Sun; title: 15. Topoisomerase I-mediated DNA relaxation as a tool to study intercalation of small molecules and supercoiled DNA / Paul Peixoto ... [et al.]; title: 16. A high-throughput assay for DNA topoisomerases and other enzymes based on DNA triplex formation / Matthew R. Burrell, Nicolas P. Burton and Anthony Maxwell; title: 17. Measurement of DNA interstrand crosslinking in individual cells using the single cell gel electrophoresis (Comet) assay / Victoria J. Spanswick, Janet M. Hartley, and John A. Hartley; title: 18. Measurement of DNA interstrand crosslinking in naked DNA using Gel-based methods / Konstantinous Kiakos, Janet M. Hartley, and John A. Hartley; title: 19. An evaluation cascade for G-quadruplex telomere targeting agents in human cancer cells / Mekala Gunaratnam and Stephen Neidle.