In vivo cellular imaging using fluorescent proteins
Subtitle: methods and protocols
By: edited by Robert M. Hoffman
Series: Methods in molecular biology -- 872
Publisher: Humana Press
Published: 2012
Place of publication: New York
Language: eng
Number of pages: 269
ISBN-13: 9781617797965; 9781617797972
Subjects: Cancer cells; Laboratory manuals; Imaging; Cancer
Description: The discovery and genetic engineering of fluorescent proteins has revolutionized cell biology. What was previously invisible in the cell often can be made visible with the use of fluorescent proteins. In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols presents state-of-the-art research that has contributed to the fluorescent protein revolution to visualize biological processes in the live animal. This volume covers an array of topics from the employment of the chick CAM model using fluorescent proteins and other fluorescent probes, to intravital fluorescent imaging, as well as 3-dimensional imaging, and design instructions on how to create new and improved far-red and infrared fluorescent proteins, to name a few. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols is the first volume in the new field of in vivo cell biology and it serves both professionals and novices with its well-honed methodologies.
Notes: Includes bibliographical references and index.