Single-molecule studies of eukaryotic DNA replication
Contributors: van Oijen, Antoine M.; Walter, Johannes C.; Hogle, James M.; Reck-Peterson, Samara L.; Rhind, Nicholas R.
Published: 2012
Language: eng
Description: DNA replication is a fundamental cellular process. However, the structure and dynamics of the eukaryotic DNA replication machinery remain poorly understood. A soluble extract system prepared from Xenopus eggs recapitulates eukaryotic DNA replication outside of a cell on a variety of DNA templates. This system has been used to reveal many aspects of DNA replication using a variety of ensemble biochemical techniques.
Notes: Keywords: eukaryotic DNA replication, Fen1, PCNA, PhADE, single molecule fluorescence.

Thesis Ph.D. Harvard University 2012