Recombinant gene expression
Subtitle: reviews and protocols
By: edited by Paulina Balbás and Argelia Lorence
Contributors: Balbás, Paulina; Lorence, Argelia
Edition: 2nd ed
Series: Methods in molecular biology -- 267; Methods in molecular biology (Clifton, N.J.) --  267
Publisher: Humana Press
Published: 2004
Place of publication: Totowa, N.J
Language: eng
Number of pages: 506
Subjects: Genetic recombination -- Laboratory manuals; Gene expression
Genres: Laboratory manuals
Other titles: Recombinant gene expression protocols
Description: Leading investigators, all of them experts on particular gene expression systems, provide a stellar set of original protocols for gene expression supported by overviews and troubleshooting guides for the biological systmes addressed. This book provides a global view of gene expression in prokaryotes, fungi, plants and animals and will be an invaluable reference in the collection of all biological science laboratories.
Notes: Rev. ed. of: Recombinant gene expression protocols / edited by Rocky S. Tuan. c1997

Includes bibliographical references and index
Table of contents: title: Host cell compatibility in protein expression / James J. Greene; title: Production of recombinant proteins / Laura A. Palomares, Sandino Estrada-Mondaca, and Octavio T. Ramírez; title: Folding promoting agents in recombinant protein production / Beatrix Fahnert; title: Back to basics / Paulina Balbás and Francisco Bolivar; title: Alpha-complementation enabled t7 expression vectors and their use for the expression of recombinant polypeptides for protein transduction experiments / Mikhail F. Alexeyev, Viktoriya V. Pastukh, Inna N. Shokolenko, and Glenn L. Wilson; title: Expression of recombinant alkaline phosphatase conjugates in Escherichia coli / Jean-Claude Boulain and Frédéric Ducancel; title: Overexpression of chromosomal genes in Escherichia coli / Fernando Valle and Noemí Flores; title: Chromosomal expression of foreign and native genes from regulatable promoters in Escherichia coli / Lu Zhou, Ke Zhang and Barry L. Wanner; title: Plasmid vectors for marker-free chromosomal insertion of genetic material in Escherichia coli / Sylvie Le Borgne, Francisco Bolivar, and Guillermo Gosset; title: Copy-control pBAC/oriV vectors for genomic cloning / Jadwiga Wild and Waclaw Szybalski; title: Copy-control tightly regulated expression vectors based on pBAC/oriV / Jadwiga Wild and Waclaw Szybalski; title: Cell-free protein synthesis with prokaryotic combined transcription-translation / James R. Swartz, Michael C. Jewett, and Kim A. Woodrow-Mumford; title: Genetic tools for the manipulation of moderately halophilic bacteria of the family halomonadaceae / Carmen Vargas and Joaquín J. Nieto; title: Gene transfer and expression of recombinant proteins in moderately halophilic bacteria / Amalia S. Afendra, Carmen Vargas, Joaquín J. Nieto and Constantin Drainas; title: Recombinant protein production in antarctic gram-negative bacteria / Angela Duilio, Maria Luisa Tutino, and Gennaro Marino; title: Recombinant protein production in yeasts / Danilo Porro and Diethard Mattanovich; title: Controlled expression of homologous genes by genomic promoter replacement in the yeast Saccharomyces cerevisiae / Kevin J. Vestrepen and Johan M. Thevelein; title: High-throughput expression in microplate format in saccharomyces cerevisiae / Caterina Holz and Christine Lang; title: High-throughput expression in microplate format in Pichia pastoris / Mewes Bötner and Christine Lang; title: Multiple gene expression by chromosomal integration and CRE-lox mediated marker recycling in saccharomyces cerevisiae / Björn Johansson and Bärbel Hahn-Hägerdal