Salamanders in regeneration research
Subtitle: methods and protocols
By: edited by Anoop Kumar, Institute of Structural and Molecular Biology, Division of Biosciences, University College London, London, UK; András Simon, Department of Cell and Molecular Biology, Karolinska Institute, Stockholm, Sweden
Series: Methods in molecular biology -- 1290; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 1290.; Springer protocols (Series)
Publisher: Humana Press
Published: 2015
Place of publication: New York
Language: eng
Number of pages: 357
ISBN-13: 9781493924943; 9781493924950
Subjects: Regeneration (Biology); Amphibians as laboratory animals; Laboratory manuals; Regeneration; Research; Animal Models; Salamanders; Genetically Modified Animals; Urodela; Laboratory Manuals
Notes: Includes bibliographical references and index.
Table of contents: title: Variation in salamanders : an essay on genomes, development, and evolution / Jeremy P. Brockes; title: Maintaining eastern newts (Notophthalmus viridescens) for regeneration research / Hans-Georg Simon and Shannon Odelberg; title: Housing and maintenance of Ambystoma mexicanum, the Mexican axolotl / Johanna E. Farkas and James R. Monaghan; title: Husbandry of Spanish ribbed newts (Pleurodeles waltl) / Alberto Joven, Matthew Kirkham, and András Simon; title: Maintaining plethodontid salamanders in the laboratory for regeneration studies / Claudia Marcela Arenas, Andrea Gómez-Molina, and Jean Paul Delgado; title: Newt lens transdifferentiation : from lentectomy to immuno-FISH / Nobuyasu Maki; title: Studying newt brain regeneration following subtype specific neuronal ablation / Matthew Kirkham and Alberto Joven; title: The accessory limb model : an alternative experimental system of limb regeneration / Tetsuya Endo, David M. Gardiner, Aki Makanae, and Akira Satoh; title: High-efficiency electroporation of the spinal cord in larval axolotl / Aida Rodrigo Albors and Elly M. Tanaka; title: Pseudotyped retroviruses for infecting axolotl / Tzu-Hsing Kuo and Jessica L. Whited; title: Thyroxine-induced metamorphosis in the axolotllotl (Ambystoma mexicanum) / Peggy S. Coots and Ashley W. Seifert; title: Generation of aneurogenic larvae by parabiosis of salamander embryos / Anoop Kumar and Jean Paul Delgado; title: In vivo modulation and quantification of microRNAs during axolotl tail regeneration / Jami R. Erickson and Karen Echeverri; title: Derivation and long-term culture of cells from newt adult limbs and limb blastemas / Patrizia Ferretti and Anoop Kumar; title: Culture and transfection of axolotl cells / Jean-François Denis, Fadi Sader, Patrizia Ferretti, and Stéphane Roy; title: Isolation and culture of neurospheres from the adult newt brain / Liyakath Ali Shahul Hameed and András Simon; title: Methods for axolotl blood collection, intravenous injection, and efficient leukocyte isolation from peripheral blood and the regenerating limb / Ryan J. Debuque and James W. Godwin; title: Assessing cardiomyocyte proliferative capacity in the newt heart and primary culture / Hans-Georg Simon and Shannon Odelberg; title: Long-term organ cultures of newt hearts / Tanja Piatkowski and Thomas Braun; title: In vitro preparation of newt inner ear sensory epithelia as a model for repair and regeneration / Ruth R. Taylor; title: Transgenesis in axolotl (Ambystoma mexicanum) / Shahryar Khattak and Elly M. Tanaka; title: Generating and identifying axolotls with targeted mutations using Cas9 RNA-guided nuclease / G. Parker Flowers and Craig M. Crews; title: Gene manipulation for regenerative studies using the Iberian ribbed Newt, Pleurodeles waltl / Toshinori Hayashi and Takashi Takeuchi; title: Transcriptomics using axolotls / S. Randal Voss, Antony Athippozhy, and M. Ryan Woodcock; title: Sal-site : research resources for the Mexican axolotl / Nour W. Al Haj Baddar, M. Ryan Woodcock, Shivam Khatri, D. Kevin Kump, and S. Randal Voss; title: Data mining in Newt-omics, the repository for omics data from the newt / Mario Looso and Thomas Braun.