Validation of putative prognostic markers for non-small cell lung carcinoma.
Published: 2007
Language: eng
Number of pages: 116
ISBN-13: 9780494274842
Description: Lung cancer is the second most diagnosed cancer in North America. Several microarray studies have reported gene expression signatures capable of classifying non-small cell carcinoma (NSCLC) patients according to prognosis. However, these signatures rarely overlap and most have not been independently validated using other methods. We therefore validated putative prognostic markers from previously published NSCLC microarray expression studies in a large independent patient cohort by real-time quantitative PCR. Two independent algorithms were used to select prognostic subsets, identifying a 6- and 3-gene classifier that could significantly segregate NSCLC patients. Next, preliminary experiments examined the potential biological role of the most robust prognostic gene in the classifiers, syntaxin 1A (STX1A). Loss of STX1A expression by RNAi had no effect on cell proliferation or cell cycle, but decreased the ability of H460 cells to migrate through collagen IV and form colonies in soft agar. STX1A represents a novel target for further investigation.
Notes: Source: Masters Abstracts International, Volume: 45-06, page: 3141.

Thesis (M.Sc.)--University of Toronto, 2007.

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