Therapeutic antibodies
Subtitle: methods and protocols
By: edited by Antony S. Dimitrov
Series: Methods in molecular biology -- 525; Springer protocols
Publisher: Humana Press; Springer [distributor]
Published: 2009
Place of publication: New York, NY; London
Language: eng
Number of pages: 585
ISBN-13: 9781934115923
Subjects: Methods; Monoclonal Antibodies; Therapeutic use; Immunoglobulins; Laboratory Manuals; Immunotherapy
Notes: Includes bibliographical references and index.
Table of contents: title: Therapeutic antibodies : current state and future trends-is a paradigm change coming soon? / Dimiter S. Dimitrov and James D. Marks -- Preparation of recombinant viral glycoproteins for novel and therapeutic antibody discovery / Yee-Peng Chan ... [et al.] -- Design of a human synthetic combinatorial library of single-chain antibodies / Limor Nahary and Itai Benhar -- Construction of a human antibody domain (V(H)) library / Weizao Chen ... [et al.] -- Generation and selection of rabbit antibody libraries by phage display / Christoph Rader -- Construction of a large naïve human phage-displayed Fab library through one-step cloning / Zhongyu Zhu and Dimiter S. Dimitrov -- Identification of target and function specific antibodies for effective drug delivery / Yu Zhou and James D. Marks -- Screening of specific internalization Fab fragment from human naive phage library by combinational bio-panning / Xin Wang and Brian B. Cao; title: Competitive antigen panning for selection of HIV-1 neutralizing human monoclonal antibodies specific for gp41 / Mei-Yun Zhang and Dimiter S. Dimitrov -- Selection of non-aggregating V(H) binders from synthetic V(H) phage-display libraries / Mehdi Arbabi-Ghahroudi, Roger MacKenzie, and Jamshid Tanha -- Isolation of full-length IgG antibodies from combinatorial libraries expressed in Escherichia coli / Yariv Mazor ... [et al.] -- Multiplexed flow cytometry : high-throughput screening of single-chain antibodies / Joanne Ayriss ... [et al.] -- Human antibody repertoires / Per-Johann Meijer ... [et al.] -- Rapid screening platform for stabilization of scFvs in Escherichia coli / Brian R. Miller, Scott M. Glaser, and Stephen J. Demarest -- In vitro antibody affinity maturation targeting germline hotspots / Mitchell Ho and Ira Pastan -- Affinity maturation by phage display / Holger Thie ... [et al.]; title: Production of chimeric heavy-chain antibodies / Jianbing Zhang, Roger MacKenzie, and Yves Durocher -- Mammalian cell display for antibody engineering / Mitchell Ho and Ira Pastan -- Improving antibody binding affinity and specificity for therapeutic development / Jenny. Bostrom ... [et al.] -- Construction and production of an IgG-like tetravalent bispecific antibody for enhanced therapeutic efficacy / Dan Lu and Zhenping Zhu -- Deimmunization of monoclonal antibodies / Tim D. Jones ... [et al.] -- Anti-CD22 onconase : preparation and characterization / Dianne L. Newton, Luke H. Stockwin, and Susanna M. Rybak -- Antibody-cytotoxic agent conjugates : preparation and characterization / Rajeeva Singh and Hans K. Erickson -- High-level production of a humanized immunoRNase fusion protein from stably transfected myeloma cells / Jürgen Krauss ... [et al.] -- Antibody fragment expression and purification / Dimana Dimitrova, Vidita Choudhry, and Christopher C. Broder; title: Scaling-up and production of therapeutic antibodies for preclinical studies / Yang Feng and Dimiter S. Dimitrov -- Antibody production by the gram-positive bacterium Bacillus megaterium / Eva Jordan ... [et al.] -- Neutralization of HIV by antibodies / Ilia Prado, Timothy R. Fouts, and Antony S. Dimitrov -- Dynamics of antibody domains studied by solution NMR / Bang K. Vu ... [et al.] -- In vivo method for establishing synergy between antibodies to epidermal growth factor receptor and vascular endothelial growth factor receptor-2 / James R. Tonra ... [et al.] -- Passive immunization with human neutralizing monoclonal antibodies against HIV-1 in macaque models : experimental approaches / Ruth M. Ruprecht -- Organizing, exploring, and analyzing antibody sequence data : the case for relational-database managers / John Owens.