RNA
Subtitle: methods and protocols
By: edited by Henrik Nielsen
Series: Springer Protocols; Methods in molecular biology -- 703
Publisher: Humana Press
Published: 2011
Place of publication: New York
Language: eng
Number of pages: 329
ISBN-13: 9781588299130; 9781597452489
Subjects: Untranslated RNA; Laboratory Manuals; Messenger RNA; Non-coding RNA
Notes: Includes bibliographical references and index.
Table of contents: title: The transcriptional landscape / Henrik Nielsen; title: Working with RNA / Henrik Nielsen; title: Synthesis of RNA by in vitro transcription / Bertrand Beckert and Benoît Masquida; title: Efficient poly(A)+ RNA selection using LNA oligo(T) capture / Nana Jacobsen, Jens Eriksen, and Peter Stein Nielsen; title: Genome browsers / Elfar Torarinsson; title: Web-based tools for studying RNA structure and function / Ajish D. George and Scott A. Tenenbaum; title: Northern blotting analysis / Knud Josefsen and Henrik Nielsen; title: Rapid amplification of cDNA ends (RACE) / Oladapo Yeku and Michael A. Frohman; title: Fractionation of mRNA based on the length of the poly(A) tail / Hedda A. Meijer and Cornelia H. de Moor; title: Analysis of mutations that influence pre-mRNA splicing / Zhaiyi Zhang and Stefan Stamm; title: S1 nuclease analysis of alternatively spliced mRNA / Martin Lützelberger and Jørgen Kjems; title: Promises and challenges in developing RNAi as a research tool and therapy / Mouldy Sioud; title: Inhibition of gene function in mammalian cells using short-hairpin RNA (shRNA) / Jørn Remi Henriksen ... [et al.]; title: Validation of RNAi by real time PCR / Knud Josefsen and Ying C. Lee; title: Profiling RNA polymerase II using the fast chromatin immunoprecipitation method / Joel Nelson, Oleg Denisenko, and Karol Bomsztyk; title: The post-transcriptional operon / Scott A. Tenenbaum, Jan Christiansen, and Henrik Nielsen; title: RIP-chip analysis : RNA-binding protein immunoprecipitation-microarray (chip) profiling / Ritu Jain ... [et al.]; title: Isolation of RNP granules / Lars Jønson, Finn Cilius Nielsen, and Jan Christiansen; title: Electrophoretic mobility shift assay for characterizing RNA-protein interaction / Keith T. Gagnon and E. Stuart Maxwell; title: Polysome analysis and RNA purification from sucrose gradients / Tomáš Mašek, Leoš Valášek, and Martin Pospíšek; title: Prediction of targets for microRNAs / Morten Lindow; title: Outsourcing of experimental work / Henrik Nielsen.