Glycobiology
Subtitle: a practical approach
By: edited by Minoru Fukuda and Akira Kobata.
Contributors: Fukuda, Minoru, 1945-; Kobata, Akira.
Series: The Practical approach series ;; 125
Publisher: IRL Press at Oxford University Press; Oxford University Press, USA
Published: 1993
Place of publication: Oxford; New York
Language: eng
Number of pages: 401
Subjects: Glycoconjugates.; Glycoproteins.; Glycoconjugates.; Glycoproteins.
Description: Glycobiology is now an area of intense research activity due to a growing awareness of the important biological roles of glycoproteins and the development of recombinant glycoproteins for therapeutic purposes. This book describes detailed protocols for all the main methods currently used to elucidate the structure and biosynthesis of glycoproteins. It covers the isolation, fractionation, and structural characterization of glycoproteins, and also of phosphatidylinositol anchors.

Glycobiology: A Practical Approach describes the essential analytical techniques required to study glycoproteins and will be an important practical guide for all researchers, including those in the pharmaceutical industry, working in this field.
Notes: Includes bibliographical references and index.
Table of contents: title: Isolation and characterization of glycoproteins / Sven R. Carlsson; title: Compositional analysis of glycoproteins / Adriana E. Manzi, Ajit Varki; title: Isolation and fractionation of N-linked oligosaccharides from glycoproteins; title: Release of N-linked oligosaccharides and fractionation by gel filtration / Akira Kobata, Tamao Endo; title: Fractionation of oligosaccharides by serial affinity chromatography with use of immobilized lectin columns / Akira Kobata, Katsuko Yamashita; title: Fractionation of glycopeptides and oligosaccharides from glycoproteins by HPLC / Yuan C. Lee, Kevin G. Rice; title: Structural characterization of oligosaccharides from glycoproteins; title: Glycosidase treatment and other methods, including methylation analysis / Akira Kobata, Seiichi Takasaki; title: FAB-MS and ES-MS of glycoproteins / Anne Dell ... [et al.]; title: Nuclear magnetic resonance spectroscopy of glycoprotein-derived carbohydrate chains / Karl Hard, Johannes F.G. Vliegenthart; title: Structural characterization of N-glycans obtained from metabolically-radiolabelled glycoproteins / Richard D. Cummings.; title: (cont) Structural characterization of mucin-type O-linked oligosaccharides / Friedrich Piller, Veronique Piller; title: Characterization of nuclear and cytoplasmic glycoproteins / William K. Berlin, John A. Hanover; title: GPI membrane anchors: isolation and analysis / Michael A.J. Ferguson; title: Suppliers of specialist items.