Identification of differentially expressed genes and proteins in the retinas of three models of inherited photoreceptor degeneration.
Published: 2004
Language: eng
Number of pages: 89
Description: The mechanisms responsible for initiating apoptosis in inherited photoreceptor degenerations (IPDs) are unknown. To identify changes in gene and protein expression characteristic of photoreceptors with an increased risk of death in IPDs, retinas were analyzed from the Rom1-/- , Rds+/- and Rho P347S  models. Affymetrix microarrays and real-time PCR were used to compare the gene expression of Rom1-/- and Rds+/- retinas with wild-type, and ProteinChipRTM SELDI-TOF-MS was used to compare the protein expression of Rom1-/- and  Rho P347S retinas with wild-type. Edn2 was dramatically up-regulated in Rds+/- (32-fold) and  Rho P347S (14-fold) retinas, and SerpinG1, as well as Osmr, Stat3, Cebpd and  A2m, which encode proteins that act in a pathway, were up-regulated in both of these models. The transcriptional increase in these genes points to novel mechanisms utilized by the retina to resist photoreceptor cell death, and implies that Rds+/- and  Rho P347S retinas are undergoing oxidative stress and inflammation.
Notes: Adviser:  Roderick R. McInnes.

Thesis (M.Sc.)--University of Toronto, 2004.

Electronic version licensed for access by U. of T. users.

Source: Masters Abstracts International, Volume: 43-03, page: 0786.

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