Protein targeting protocols
By: edited by Mark van der Giezen
Contributors: Van der Giezen, Mark
Edition: 2nd ed
Series: Methods in molecular biology -- 390; Methods in molecular biology (Clifton, N.J.) --  390
Publisher: Humana Press
Published: 2007
Place of publication: Totowa, N.J
Language: eng
Number of pages: 501
ISBN-13: 9781588297020; 9781597454667
Subjects: Protein binding -- Research -- Laboratory manuals
Genres: Laboratory manuals
Description: Provides state-of-the-art information on the fast moving fields of import machinery of mitochondria and plastids, as well as a detailed protocol on the movement of protein complexes in bacterial membranes using fluorescent recovery after photobleaching. Bacterial protein targeting protocols using the Sec-system, type-V secretion apparatus, and the Tat-pathway are described, as is a periplasmic targeting protocol. Chapters also include bioinformatics methods to guide readers through the ever-increasing number of in silico tools currently available. Editor Mark van der Giezen has succeeded in including targeting protocols from different systems, including animal, plant, fungal, and protist models, providing insights into the intricate challenges presented by these diverse systems.
Notes: Includes bibliographical references and index