Cell-cell interactions
Subtitle: methods and protocols
By: edited by Sean P. Colgan.
Contributors: Colgan, Sean P.
Series: Methods in molecular biology,; v. 341; Methods in molecular biology (Clifton, N.J.) ;; v. 341.
Publisher: Humana Press
Published: 2006
Place of publication: Totowa, N.J
Language: eng
Number of pages: 286
Subjects: Cell interaction; Cell junctions; Epithelial cells; Cell Communication -- physiology; Endothelial Cells -- physiology; Epithelial Cells -- physiology; Tight Junctions -- physiology
Description: A versatile collection of readily reproducible cell-cell interaction assays for uncovering cellular interactions at the molecular level, both in vitro and in vivo. The protocols cover a diverse set of cell-cell interaction models in both normal and pathological states, are readily adaptable to nearly any cell type and organ system, and include primary data and outcome analysis. In addition, the protocols follow the successful Methods in Molecular Biologyâ„¢ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Notes: Includes bibliographical references and index.
Table of contents: title: Blood/brain barrier and cell/cell interactions: methods for establishing in vitro models of the blood/brain barrier and transport measurements / Michael Aschner ... [et al.]; title: Interactions of intestinal epithelial cells with bacteria and immune cells: methods to characterize microflora and functional consequences / Geraldine Canny, Alexander Swidsinski, and Beth A. McCormick; title: The role of junctional adhesion molecules in interactions between vascular cells / Triantafyllos Chavakis and Valeria Orlova; title: Cell/cell interactions on solid matrices / Nancy A. Louis, Dionne Daniels, and Sean P. Colgan; title: Cell/cell interactions in the kidney: inducible expression of mutant G protein -subunits in MDCK cells for studies of epithelial cell junction structure and function / Ernesto Sabath and Bradley M. Denker; title: Nucleotide metabolism and cell/cell interactions / Holger K. Eltzschig ... [et al.]; title: Analysis of mammalian sperm/egg membrane interactions during in vitro fertilization / Genevieve B. Wortzman, Allison J. Gardner, and Janice P. Evans; title: Collagen gel contraction assay / Peter Ngo ... [et al.]; title: Methods to assess tissue permeability / Juan C. Ibla and Joseph K. Khoury; title: A biochemical method for tracking down cholera toxin transport from plasma membrane to golgi and endoplasmic reticulum / Heidi E. DeLuca and Wayne I. Lencer; title: Isolation and culture of murine heart and lung endothelial cells for in vitro model systems / Yaw-Chyn Lim and Frances W. Luscinskas; title: Expression cloning of signaling proteins regulated by cell adhesion / Michelle L. Matter and Joe W. Ramos; title: Assays for the calcium sensitivity of desmosomes / Anita J. Merritt, Anthea Scothern, and Tanusree Bhattacharyya; title: Tight junctions and cell/cell interactions / Markus Utech, Matthias Bruwer, and Asma Nusrat; title: Simultaneous immunofluorescence detection of cadherin and -catenin in mouse tissue sections / Nobuko Obara; title: In vitro neutrophil transepithelial migration / Winston Y. Lee ... [et al.]; title: The blot running assay: a method for identifying adhesion molecules mediating binding under shear conditions / Robert Sackstein and Robert Fuhlbrigge; title: Cell/cell interaction in the transcellular biosynthesis of novel -3-derived lipid mediators / Nan Chiang and Charles N. Serhan; title: Isolation and analysis of lipid rafts in cell/cell interactions / Aimee Landry and Ramnik Xavier.