Enzyme Purification and Related Techniques: Volume 22
Subtitle: Enzyme Purification and Related Techniques (Methods in Enzymology)
Contributors: Nathan P. Kaplan (Editor); Nathan P. Colowick (Editor); William B. Jakoby (Editor)
Publisher: Academic Press
Published: June 28, 1971
Language: eng
Number of pages: 648
ISBN-13: 9780121818852
First sentence: Buffers of any desired pH can be prepared quickly by mixing together the component stock solutions of a suitable buffer acid and that of its conjugate base in appropriate amounts according to Eq. (1), where the (1) pK represents the apparent pK at the ionic strength of the buffer and fd is the degree of dissociation, i.e., the fraction of total buffer in the dissociated form, A.