HPLC methods for clinical pharmaceutical analysis
Subtitle: a user's guide
By: Hermann Mascher
Edition: 1st ed.
Publisher: Wiley-VCH
Published: 2012
Place of publication: Weinheim
Language: eng
Number of pages: 247
ISBN-13: 9783527331291
Subjects: High performance liquid chromatography; Drugs; Analysis; Pharmaceutical technology
Description: The author describes all steps of a routine pharmaceutical analysis in an intuitive fashion and explains how to develop robust and efficient methods for any clinical compound. A reference appendix contains tried and tested methods for 100 freqently encountered pharmaceuticals.
Notes: Includes bibliographical references and index.
Table of contents: title: 1.Introduction; title: 1.1.First Question: Determination of Ibuprofen in Plasma; title: 1.2.Second Question: Determination of Tryptophan in Urine; title: 1.3.Third Question: Determination of Paclitaxel in Tissue; title: 2.Planning of Analyses; title: 2.1.Introduction; title: 2.2.Limit of Detection (LOD) and Determination (LLOQ); title: 2.3.Detectors; title: 2.4.Structure of the Analyte; title: 2.5.Solubility of an Analyte; title: 2.6.Selection of the Detector; title: 3.Sample Preparation; title: 3.1.Dilution; title: 3.2.Protein Precipitation, Overview; title: 3.3.Extraction; title: 4.HPLC Separation; title: 4.1.HPLC Pumps; title: 4.2.Degasser; title: 4.3.Injector; title: 4.4.HPLC Columns; title: 5.Detection; title: 5.1.Detection in the Pharmaceutical/Bioanalytical Area; title: 5.2.Detection in the "Clinical Area" (Therapy Control/Compliance); title: 6.Chemical Derivatization for Detection Enhancement; title: 7.Validation Concepts; title: 7.1.Introduction; title: 7.2.Realization of the FDA Guideline; title: 8.Practical Hints Concerning Stability, Destruction and Degradation Products; title: 9.Metabolites; title: 10.Internal Standards; title: 11.Case Studies with Intensive Discussion for Each Substance; title: 11.1.Acetylcarnitine in Plasma; title: 11.2.Acetylcysteine in Plasma; title: 11.3.Acyclovir in Plasma and Urine; title: 11.4.Caffeine in Plasma; title: 11.5.Diazepam in Plasma; title: 11.6.Diclofenac in Plasma; title: 11.7.Dihydralazine in Plasma; title: 11.8.Duramycin (Moli1901) in Plasma; title: 11.9.Fluticasone Propionate in Plasma; title: 11.10.Hydroxytriamterene Sulfate and Triamterene in Plasma and Urine; title: 11.11.Ibuprofen in Plasma (also Enantiomeric Separation); title: 11.12.Minocycline in Plasma; title: 11.13.Norfloxacine in Plasma and Urine; title: 11.14.Paclitaxel in Plasma, Urine and Tissue; title: 11.15.Paracetamol (Acetaminophen) in Plasma; title: 11.16.Pimelic Acid in Plasma and Urine; title: 11.17.8-Prenylnaringenin in Plasma and Different Types of Tissues; title: 11.18.Silibinin in Plasma; title: 11.19.Valnemulin in Plasma, Different Tissue Types and in Animal Feed; title: 11.20.Vitamin B1 (Total Thiamine) in Plasma; title: Table 11.1 Recommended books; title: Table 11.2 Properties of substances; title: Appendix; title: Short Description of Determination for about 100 Substances; title: Substances listed in the Appendix.