Biophotonics, Part B (Methods in Enzymology, Volume 361) (Methods in Enzymology)
Contributors: Gerard Marriott (Editor); Ian Parker (Editor)
Publisher: Academic Press
Published: February 2003
Language: eng
Number of pages: 590
ISBN-13: 9780121822644
First sentence: Total internal reflection fluorescence (TIRF) microscopy (also called "evanescent wave microscopy") provides a means to selectively excite fluorophores in an aqueous or cellular environment very near a solid surface (within 100 nm) without exciting fluorescence in regions farther from the surface.