Receptor signal transduction protocols
By: edited by Gary B. Willars and R.A. John Challiss
Edition: 3rd ed.
Series: Springer protocols; Methods in molecular biology -- 746; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 746.
Publisher: Springer
Published: 2011
Place of publication: New York
Language: eng
Number of pages: 479
ISBN-13: 9781617791253; 9781617791260
Subjects: Methodology; Cell receptors; Cellular signal transduction; Signal Transduction; Laboratory Manuals; Physiology; Research
Description: [Publisher-supplied data] As our understanding of G protein-coupled receptor (GPCR) signal transduction continues to grow, we cannot help but be struck by the emerging complexity and the ability of this receptor superfamily to continually surprise us as new facets are discovered. In this third edition of Receptor Signal Transduction Protocols, the expert contributors have taken into account the constant evolution of the GPCR field and dealt with methods that allow researchers to address many of the important rising issues. Serving as a companion to the previous editions, this volume contains thorough revisions of some core chapters, the complete rewriting of others to encompass new technological developments since the publication of the prior editions, and the commissioning of brand new, cutting-edge chapters to expand on previous coverage. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to the respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Receptor Signal Transduction Protocols, Third Edition serves the scientific community with a broad collection of the current, practical methodologies in this highly promising field.
Notes: Includes bibliographical references and index.
Table of contents: title: Inducible expression of G protein-coupled receptors in transfected cells  -- Beryl Koener and Emmanuel Hermans; title: Using the Flp-In T-Rex system to regulate GPCR expression  -- Richard J. Ward, Elisa Alvarez-Curto, and Graeme Milligan; title: Viral infection for GPCR expression in eukaryotic cells  -- Antonio Porcellini, Luisa Iacovelli, and Antonio De Blasi; title: Generation of epitope-tagged GPCRs  -- Yan Huang and Gary B. Willars; title: Use of site-directed mutagenesis to study GPCRs  -- Alex C. Conner ... [et al.]; title: Approaches to study GPCR regulation in native systems  -- Jonathon M. Willets; title: Heterologous expression of GPCRs in fission yeast  -- John Davey and Graham Ladds; title: Radioligand binding methods for membrane preparations and intact cells  -- David B. Bylund and Myron L. Toews; title: Quantification of GPCR mRNA using real-time RT-PCR  -- Trond Brattelid and Finn Olav Levy; title: Determining allosteric modulator mechanism of action : integration of radioligand binding and functional assay data  -- Christopher J. Langmead; title: Design and use of fluorescent ligands to study ligand-receptor interactions in single living cells  -- Stephen J. Briddon, Barrie Kellam, and Stephen J. Hill; title: Examining site-specific GPCR phosphorylation  -- Adrian J. Butcher, Andrew B. Tobin, and Kok Choi Kong; title: Ubiquitination of GPCRs  -- Adriana Caballero and Adriano Marchese; title: [35S]GTPgS binding as an index of total G-protein and Ga-subtype-specific activation by GPCRs  -- Rajendra Mistry, Mark R. Dowling, and R. A. John Challiss; title: Using calcium imaging as a readout of GPCR activation  -- Martin D. Bootman and H. Llewelyn Roderick; title: Measuring spatiotemporal dynamics of cyclic AMP signaling in real-time using FRET-based biosensors  -- Frank Gesellchen ... [et al.]; title: Determining the activation of Rho as an index of receptor coupling to G12/13 proteins  -- Michio Nakaya ... [et al.]; title: Use of translocating fluorescent biosensors for real-time monitoring of GPCR-mediated signaling events  -- Carl P. Nelson and R.A. John Challiss; title: Study of GPCR-protein interactions using gel overlay assays and glutathione-S-transferase-fusion protein pull-downs  -- Ashley E. Brady ... [et al.]; title: Study of GPCR-protein interactions by BRET  -- Martina Kocan and Kevin D.G. Pfleger; title: Time resolved FRET strategy with fluorescent ligands to analyze receptor interactions in native tissues : application to GPCR oligomerization  -- Martin Cottet ... [et al.]; title: Peptide affinity purification for the isolation and identification of GPCR-associated protein complexes  -- Pascal Maurice, Avais M. Daulat, and Ralf Jockers; title: Tandem affinity purification and identification of GPCR-associated protein complexes  -- Avais M. Daulat, Pascal Maurice, and Ralf Jockers; title: Identification of GPCR localization in detergent resistant membranes  -- Ranju Kumari and Anna Francesconi; title: Analysis of GPCR localization and trafficking  -- James N. Hislop and Mark von Zastrow; title: Statistical methods in research -- Domenico Spina.