Elastin polypeptide-coated small-diameter vascular grafts.
Published: 2004
Language: eng
Number of pages: 170
Description: In this work, we examined the feasibility of using recombinant human elastin polypeptide (EP) to coat e-PTFE vascular grafts. First, EP was cross-linked using: glutaraldehyde, lysine diisocyanate, and pyrolloquinoline quinone. Second, we attempted to cross-link EP coatings applied to e-PTFE vascular grafts. Acceptable coatings were achieved through passive adsorption from 70% ethanol and DMSO solutions. Unfortunately, coating cross-linking with glutaraldehyde or pyrolloquinoline quinone resulted in an inappropriate topography for the desired application. The use of gamma irradiation resulted in an ideal cross-linked EP coating. However, gamma irradiation is not recommended with e-PTFE. Finally, we preliminarily assessed the smooth muscle cell (SMC) and endothelial cell (EC) response to our EPs. [3H] thymidine uptake experiments showed slower SMC proliferation on EP compared to growth on gelatin. In addition, our analysis shows lower collagen and elastase secretions indicating that the presence of EP does not appear to upregulate the cells in culture.
Notes: Adviser:  Kimberly Woodhouse.

Thesis (M.A.Sc.)--University of Toronto, 2004.

Electronic version licensed for access by U. of T. users.

Source: Masters Abstracts International, Volume: 43-03, page: 0909.

MICR copy on microfiche (2 microfiches).