MicroRNAs and the immune system
Subtitle: methods and protocols
By: edited by Silvia Monticelli
Series: Springer protocols; Methods in molecular biology -- 667; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 667.
Publisher: Humana Press
Published: 2010
Place of publication: New York, NY
Language: eng
Number of pages: 330
ISBN-13: 9781607618102
Subjects: Small interfering RNA; Immunology; Immune system; Laboratory Manuals; Laboratory manuals; Physiology; MicroRNAs
Description: In recent years, the critical role of microRNAs has been revealed within the biology of cells that constitute the immune system. In MicroRNAs and the Immune System: Methods and Protocols, expert researchers explore the latest techniques for studying miRNA expression, including the most up-to-date data on splinted ligation and qRT-PCR assays, as well as high-throughput profiling through cloning, deep sequencing, and microarrays. Chapters outline methods to study miRNA functions in various cell types from a single cell type level to entire model organisms, and present studies of miRNAs in the context of viruses and the immune response. Tools are also provided to help navigate bioinformatics databases on miRNAs and their targets. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Contemporary and innovative, MicroRNAs and the Immune System: Methods and Protocols is an essential handbook for immunologists, biochemists, and molecular biologists.
Notes: Includes bibliographical references and index.

Also available online.
Table of contents: title: A rapid, quantitative assay for direct detection of microRNAs and other small RNAs using splinted ligation / Sangpen Chamnongpol, Patricia A. Maroney, and Timothy W. Nilson; title: Normalization of microRNA quantitative RT-PCR data in reduced scale experimental designs / Gary J. Latham; title: MicroRNA detection in bone marrow cells by LNA-FISH / Silvana Debernardi and Amanda Dixon-McIver; title: Measuring microRNA expression in size-limited FACS-sorted and microdissected samples / Kai P. Hoefig and Vigo Heissmeyer; title: MicroRNA cloning from cells of the immune system / Haoquan Wu, Joel Neilson, and N. Manjunath; title: High-throughput profiling in the hematopoietic system / Muller Fabbri, Riccardo Spizzo, and George A. Calin; title: Construction of small RNA cDNA libraries for deep sequencing / Molly F. Thomas and K. Mark Ansel; title: MicroRNA-profiling in formalin-fixed paraffin-embedded specimens / Ulrich Lehmann; title: Expression of miRNAs in lymphocytes : a review / Raquel Malumbres and Izidore S. Lossos; title: Mouse models for miRNA expression : the ROSA26 locus / Stefano Casola; title: Regulation of monocytopoiesis by microRNAs / Laura Fontana, Antonio Sorrentino, and Cesare Peschle; title: MicroRNA activity in B lymphocytes / Virginia G. de Yébenes and Almudena R. Ramiro; title: Isolation and characterization of microRNAs of human mature erythrocytes / Carolyn Sangokoya, Gregory LaMonte, and Jen-Tsan Chi; title: Stable overexpression of miRNAs in bone marrow-derived murine mast cells using lentiviral expression vectors / Ramon J. Mayoral and Silvia Monticelli; title: Monitoring microRNA activity and validating microRNA targets by reporter-based approaches / Alessia Baccarini and Brian D. Brown; title: Lentivirus-mediated antagomir expression / Ewa Surdziel, Matthias Eder, and Michaela Scherr; title: Solution structure of miRNA:mRNA complex / Mirko Cevec and Janez Plavec; title: MiRNA editing / Dylan E. Dupuis and Stefan Maas; title: Computational prediction of microRNA targets / Xiaowei Wang; title: Large-scale integration of microRNA and gene expression data for identification of enriched microRNA-mRNA associations in biological systems / Preethi H. Gunaratne ... [et al.]; title: Identification and validation of the cellular targets of virus-encoded microRNAs / Kin-Hang Kok, Ting Lei, and Dong-Yan Jin.