Immunochemical protocols
By: edited by Robert Burns
Contributors: Burns, Robert, 1959-
Edition: 3rd ed
Series: Methods in molecular biology -- 295; Methods in molecular biology (Clifton, N.J.) --  295
Publisher: Humana Press
Published: 2005
Place of publication: Totowa, N.J
Language: eng
Number of pages: 317
Subjects: Immunochemistry -- Laboratory manuals
Genres: Laboratory manuals
Notes: Includes bibliographical references and index
Table of contents: title: Immunization strategies for antibody production / Robert Burns; title: Synthetic peptides as antigens for antibody production / David C. Hancock and Nicola J. O'Reilly; title: Production of polyclonal antibody in rabbits / David C. Hancock and Nicola J. O'Reilly; title: Making hybridomas / Robert Burns; title: Growing hybridomas / Gary Entrican and Gareth Young; title: Phage display vectors for the in vitro generation of human antibody fragments / Michael Hust and Stefan Dobel; title: Purification of antibodies and preparation of antibodies fragments / Graham D. Kemp; title: Conjugation of antibodies to alkaline phosphatase / G. Brian Wisdom; title: Conjugation of antibodies to horseradish peroxidase / G. Brian Wisdom; title: Conjugation of antibodies to fluorescein or rhodamine / G. Brian Wisdom; title: Conjugation of antibodies to biotin / James D. Hirsch and Rosaria P. Haughland; title: Preparation of colloidal gold probes / David Hughes; title: Immunogold probes in light microscopy / David Hughes; title: Immunoelectron microscope techniques in plant virus diagnosis / Daphne M. Wright; title: Dual-antibody sandwich enzyme-linked immunosorbent assay / William Jordan; title: Competitive enzyme-linked immunosorbent assay / William Jordan; title: Immunoblotting techniques / Barbara Magi and Sabrina Liberatori; title: Epitope mapping / Glenn E. Morris; title: Generation and testing of immunoaffinity columns / Weilin L. Shelver; title: Immunocapture-polymerase chain reaction  / Vincent Mulholland; title: Magnetic cell sorting / Maciej Zborowski and Jeffrey J. Chalmers; title: Cell enrichment and immunochemical staining / Oscar R. Lara and Jeffrey J. Chalmers