Cancer cell culture
Subtitle: methods and protocols
By: edited by Ian A. Cree
Edition: 2nd ed.
Series: Methods in molecular biology -- v. 731; Springer protocols; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 731.
Publisher: Humana Press
Published: 2011
Place of publication: Totowa, N.J
Language: eng
Number of pages: 502
ISBN-13: 9781617790799; 9781617790805
Subjects: Cultured Tumor Cells; Cancer cells; Laboratory manuals; Laboratory Techniques and Procedures
Notes: Includes bibliographical references and index.
Table of contents: title: Xenotransplantation of breast cancers; title: Feeder layers: Co-culture with nonneoplastic cells; title: Drug design and testing: Profiling of antiproliferative agents for cancer therapy using a cell-based methyl-[3h]-thymidine incorporation assay; title: Transfection and DNA-mediated gene transfer; title: Analysis of drug interactions; title: Development of rituximab-resistant B-NHL clones: An in vitro model for studying tumor resistance to monoclonal antibody-mediated immunotherapy; title: Proteomic evaluation of cancer cells: Identification of cell surface proteins; title: Measuring gene expression from cell cultures by quantitative reverse-transcriptase polymerase chain reaction; title: Expression analysis of homeobox genes in leukemia/lymphoma cell lines; title: Flow cytometric DNA analysis of human cancers and cell lines; title: Angiogenesis assays; title: Cell migration and invasion assays; title: Molecular breakpoint analysis of chromosome translocations in cancer cell lines by long distance inverse-PCR; title: Measurement of DNA damage in individual cells using the Single Cell Gel Electrophoresis (Comet) assay; title: Purification of annexin V and its use in the detection of apoptotic cells; title: Real-time cytotoxicity assays; title: Differential staining cytotoxicity assay: A review; title: Cell sensitivity assays: The ATP-based tumor chemosensitivity assay; title: Cell sensitivity assays: The MTT assay; title: Cellular chemosensitivity assays: An overview; title: Isolation of endothelial cells from human tumors; title: Isolation of inflammatory cells from human tumours; title: Establishment and culture of leukemia-lymphoma cell lines; title: Isolation and culture of ovarian cancer cells and cell lines; title: Isolation and culture of squamous cell carcinoma lines; title: Isolation and culture of melanoma and naevus cells and cell lines; title: Isolation and culture of colon cancer cells and cell lines; title: Generation of lung cancer cell line variants by drug selection or cloning; title: Quality assurance and good laboratory practice; title: Elimination of mycoplasmas from infected cell lines using antibiotics; title: Detecting mycoplasma contamination in cell cultures by polymerase chain reaction; title: Cell culture contamination; title: Cytogenetic analysis of cancer cell lines; title: Online verification of human cell line identity by STRDNA typing; title: Characterization and authentication of cancer cell lines: An overview; title: Storage of cell lines; title: Principles of cancer cell culture; title: Cancer biology.