Evidence for a role of Src tyrosine kinase in high glucose-induced collagen accumulation in mesangial cells.
Published: 2005
Language: eng
Number of pages: 162
Description: Src, the prototype of cellular members of Src family non-receptor tyrosine kinases, is involved in cell proliferation, differentiation, metabolism, motility and adhesion. However, its role in high glucose-induced diabetic complications has not been reported. Our purpose was to investigate the role of Src kinase in the mechanism by which high glucose (HG) causes collagen IV accumulation in cultured rat mesangial cells (MCs). Exposure of MCs to HG induced Src kinase activation. This effect was reduced by both PP2 and SU6656 inhibitors. HG activated the MAP kinase family members, p38 and ERK 1/2 but not JNK. This effect was blocked by PP2 and SU6656 inhibitors. HG-increased collagen IV accumulation in MCs was blocked by the Src kinase inhibitors. Collagen IV expression and phosphorylation of ERK 1/2 were reduced in transfected mesangial cells with dominant negative (DN)-Src cDNA in HG. These data suggest that HG mediates collagen accumulation via a Src-MAPK signaling pathway.
Notes: Source: Masters Abstracts International, Volume: 44-02, page: 0766.

Thesis (M.Sc.)--University of Toronto, 2005.

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