DNA replication
Subtitle: methods and protocols
By: edited by Sonya Vengrova, Coventry, UK, Jacob Dalgaard, Warwick Medical School, University of Warwick, Coventry, UK
Edition: Second edition.
Series: Methods in molecular biology -- 1300; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- 1300.; Springer protocols (Series)
Publisher: Humana Press
Published: 2015
Place of publication: New York
Language: eng
Number of pages: 295
ISBN-13: 9781493925957; 9781493925964
Subjects: DNA replication; Laboratory manuals; DNA Replication; Methodology; Research
Work titles: DNA replication (Vengrova)
Description: Updated and revised, this thorough volume covers a range of methods focusing on systems, including mammalian, yeast, bacterial and archaeal. This second edition of DNA Replication: Methods and Protocols describes approaches to analyze whole genomes to single molecules, as well as both in vivo and in vitro experiments. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, DNA Replication: Methods and Protocols, Second Edition provides a collections of methods intended for newcomers to this research field and for established laboratories.
Notes: Includes bibliographical references and index.
Table of contents: title: A High-Throughput Confocal Fluorescence Microscopy Platform to Study DNA Replication Stress in Yeast Cells; title: Microscopy Techniques to Examine DNA Replication in Fission Yeast; title: High-Resolution Analysis of Mammalian DNA Replication Units; title: Analyzing the Dynamics of DNA Replication In Mammalian Cells Using DNA Combing; title: Measuring DNA Content by Flow Cytometry in Fission Yeast; title: Incorporation of Thymidine Analogs for Studying Replication Kinetics in Fission Yeast; title: EdU Incorporation for FACS and Microscopy Analysis of DNA Replication in Budding Yeast; title: Determination of Deoxyribonucleoside Triphosphate Concentrations in Yeast Cells by Strong Anion-Exchange High-Performance Liquid Chromatography Coupled with Ultraviolet Detection; title: Measuring Ribonucleotide Incorporation Into DNA In Vitro and In Vivo; title: Detection and Sequencing of Okazaki Fragments In S. cerevisiae; title: ChIP-Seq to Analyze the Binding of Replication Proteins to Chromatin; title: Chromatin Immunoprecipitation to Detect DNA Replication and Repair Factors; title: Molecular Genetic Methods to Study DNA Replication Protein Function in Haloferax volcanii, A Model Archaeal Organism; title: Single-Molecule Observation of Prokaryotic DNA Replication; title: Analyzing the Response to Dysfunction Replication Forks Using the RTS1 Barrier System in Fission Yeast; title: Purification of Restriction Fragments Containing Replication Intermediates from Complex Genomes for 2-D Gel Analysis; title: Isolation of Restriction Fragments Containing Origins of Replication from Complex Genomes.