Optimization in drug discovery
Subtitle: in vitro methods
By: edited by Zhengyin Yan, Gary W. Caldwell.
Contributors: Yan, Zhengyin.; Caldwell, Gary, 1953-
Series: Methods in pharmacology and toxicology
Publisher: Humana Press
Published: 2004
Place of publication: Totowa, N.J
Language: eng
Number of pages: 418
Subjects: Pharmaceutical chemistry -- Laboratory manuals; Physical biochemistry -- Laboratory manuals; Drugs -- Testing -- Laboratory manuals; Drug Evaluation, Preclinical -- methods
Genres: Laboratory manuals.
Notes: Includes bibliographical references and index.
Table of contents: title: pKa, solubility and lipophilicity: assessing physicochemical properties of lead compounds / Yushen Guo and Hong Shen; title: Use of caco-2 cell monolayers to study drug absorption and metabolism / Ming Hu ... [et al.]; title: Absorption screening using the PAMPA approach / Jeffrey A. Ruell and Alex Avdeef; title: In situ single-pass perfused rat intestinal model for absorption and metabolism / Eun Ju Jeong ... [et al.]; title: In vitro permeation study with bovine brain microvessel endothelial cells / Sung-Hack Lee ... [et al.]; title: An enzymatic microplate assay for testing P-glycoprotein substrates and inhibitors / S. Orlowski, J. Nugier and Eric Ezan; title: Evaluation of drug-transporter interactions using in vitro cell models / Yaming Su and Patrick J. Sinko; title: Plasma protein-binding methods in drug discovery / Lucinda H. Cohen; title: Isothermal titration calorimetry characterization of drug-binding energetics to blood proteins / Gary W. Caldwell and Zhengyin Yan; title: Metabolic stability assessed by liver microsomes and hepatocytes / David C. Ackley, Kevin T. Rockich, and Timothy R. Baker; title: In vitro drug metabolite profiling using hepatic S9 and human liver microsomes / Wu-Nan Wu and Linda A. McKown; title: In vitro identification of UDP-glucuronosyltransferases involved in drug metabolism / Michael H. Court; title: In vitro CYP induction in human hepatocytes / Daniel R. Mudra and Andrew Parkinson; title: High-throughput screening of human cytochrome P450 inhibitors using fluorometric substrates: methodology for 25 enzyme/substrate pairs / David M. Stresser; title: Evaluation of cytochrome P450 inhibition in human liver microsomes / Zhengyin Yan and Gary W. Caldwell; title: Identification of CYP mechanism-based inhibitors / Amin A. Nomeir, Jairam Palamanda, and Leonard Favreau; title: Detection of DNA adducts by 32P-postlabeling analysis / Naomi Suzuki, Padmaja M. Prabhu, and Shinya Shibutani; title: Covalent DNA adduct formation mediated by cytochrome P450 / Marie Stiborová; title: Application of in vitro comet assay for genotoxicity testing / Bojana Zegura and Metka Filipic; title: Assessing DNA damage using a reporter gene system / Xuming Jia and Wei Xiao; title: Improvement of the Ames test using human liver S9 preparation / Atsushi Hakura, Satoshi Suzuki, and Tetsuo Satoh; title: Screening for chemical mutagens using the mouse lymphoma assay / Tao Chen and Martha M. Moore; title: A high throughput binding assay for HERG / Keith Finlayson and John Sharkey; title: In vitro drug metabolism: thiol conjugation / Wei Tang and Randy R. Miller; title: In vitro screening assay of the reactivity of acyl glucuronides / Sébastien Bolze.