Antibody phage display
Subtitle: methods and protocols
Edition: 2nd ed. / edited by Robert Aitken.
Series: Methods in molecular biology -- 562; Springer protocols
Publisher: Humana Press
Published: 2009
Place of publication: Dordrecht; New York
Language: eng
Number of pages: 240
ISBN-13: 9781603273015; 9781603273022
Subjects: Monoclonal Antibodies; Laboratory Manuals; Bacteriophages; Monoclonal antibodies
Notes: Includes bibliographical references and index.

Mode of access: Internet.
Table of contents: title: Antibody phage display: overview of a powerful technology that has quickly translated to the clinic / B. Kotlan and M.C. Glassy; title: Design and construction of synthetic phage-displayed Fab libraries / J. Bostrom and G. Fuh; title: Affinity isolation of antigen-specific circulating B cells for generation of phage display-derived human monoclonal antibodies / H.J. Ditzel; title: Construction of phage antibody repertoires from the blood of West Nile virus-infected donors / M. Throsby and J. de Kruif; title: Anti-beta(2)GP-I and anti-prothrombin antibodies generated by phage display / J. Cabiedes and A.R. Cabral; title: The generation and selection of single-domain, v region libraries from nurse sharks / M.F. Flajnik and H. Dooley; title: The isolation of scFvs against small target molecules / I. Shaw and M. Kane; title: Display and selection of scFv antibodies on HEK-293T cells / M. Ho and I. Pastan; title: Isolation of scFvs that inhibit the NS3 protease of hepatitis C virus by a combination of phage display and a bacterial genetic screen / L. Nahary, A. Trahtenherts and I. Benhar; title: Guided selection methods through chain shuffling / B. Guo-Qiang and H. Xian-Li; title: Sequential antigen panning for selection of broadly cross-reactive HIV-1-neutralizing human monoclonal antibodies / M.Y. Zhang and D.S. Dimitrov; title: Selection of antibodies able to rapidly enter mammalian cells / M.A. Poul; title: Identification of the specificity of isolated phage display single-chain antibodies using yeast two-hybrid screens / N. Rasmussen and H.J. Ditzel; title: Generation of bispecific and tandem diabodies / S.M. Kipriyanov; title: Intrabody expression in eukaryotic cells / L. Guglielmi and P. Martineau; title: High-level periplasmic expression and purification of scFvs / S.M. Kipriyanov; title: Expression of single-chain Fv fragments in E. coli cytoplasm / L. Guglielmi and P. Martineau; title: High-level expression of a phage display-derived scFv in Pichia pastoris / L.M. Damasceno ... [et al.].