Type IV collagen in development and disease
Subtitle: towards gene therapy for X-linked Alport syndrome.
Published: 2004
Language: eng
Number of pages: 317
Description: X-linked Alport syndrome (XLAS) is a disorder characterized by nephropathy and deafness that is caused by mutations in the COL4A5  gene, which encodes the alpha5(IV) collagen chain. A canine model of XLAS was studied to elucidate the pathogenesis of this disease in humans and to establish the feasibility of gene therapy for its treatment. The temporal and spatial expression of the alpha1(IV)--alpha6(IV) chains in basement membranes (BMs) of the kidney, inner ear and testis was documented and correlated to normal and pathologic changes in BM ultrastructure and function. The loss of the alpha3(IV), alpha4(IV) and alpha6(IV) chains from all BMs in affected dogs stems from a failure at the level of protein assembly, and is permissive to normal development in all tissues studied. In affected kidney, the alpha1(IV) and alpha2(IV) chains are sufficient for normal glomerular structure and function on a short-term basis, but not for their long-term maintenance. In normal inner ear, changes in the distribution of the alpha3(IV)--alpha5(IV) chains occurred co-incident with, but were not required for the acquisition of mature auditory function, and their localization led to a new theory on the etiology of deafness in XLAS. In affected testis, the absence of the alpha3(IV)--alpha6(IV) chains leads to structural changes of the seminiferous tubule BM that impairs, but does not prevent normal function. A cDNA encoding canine alpha5(IV) collagen was cloned and expressed in vitro in 293 cells. By Northern blotting, an alpha5(IV) mRNA transcript of &sim;5.2 kb was expressed and the recombinant protein was detected by immunocytochemistry. The alpha5(IV) chain was secreted as a &sim;190 kDa monomer that did not form homotrimers, nor heterotrimers with the endogenous alpha1(IV) or alpha2(IV) chains, a finding consistent with its requirement for the alpha3(IV) and alpha4(IV), or alpha6(IV) chains for triple helical assembly. An adenoviral vector for the alpha5(IV) chain was used to express the transgene in smooth muscle of affected dogs, which lacks the alpha5(IV) and alpha6(IV) chains. Expression of the alpha5(IV) transgene rescued expression of the alpha6(IV) chain and both assumed a BM distribution. This finding provides 'proof of principle' that gene therapy for XLAS may be feasible.
Notes: Adviser:  Paul S. Thorner.

Thesis (Ph.D.)--University of Toronto, 2004.

Electronic version licensed for access by U. of T. users.

Source: Dissertation Abstracts International, Volume: 65-10, Section: B, page: 5091.