Patch-clamp methods and protocols
By: edited by Marzia Martina, Department of Translational Bioscience, National Research Council of Canada, Ottawa, ON, Canada,   Stefano Taverna, Department of Neuroscience & Brain Technologies, Italian Institute of Technology, Genoa, Italy
Edition: Second edition.
Series: Methods in molecular biology -- 1183; Springer protocols
Publisher: Humana Press
Published: 2014
Place of publication: New York
Language: eng
Number of pages: 403
ISBN-13: 9781493910953; 9781493910960
Copyright date: 2014
Subjects: Patch-clamp techniques (Electrophysiology)
Notes: Includes bibliographical references and index.
Table of contents: title: 1. History of electrophysiology and the patch clamp / Alexei Verkhratsky and Vladimir Parpura; title: 2. Whole-cell patch-clamp analysis of recombinant NMDA receptor pharmacology using  brief glutamate applications / Nathan G. Glasgow and Jon W. Johnson; title: 3. Patch clamp combined with voltage/concentration clamp to determine the kinetics and voltage dependency on N-methyl-D-aspartate (NMDA) receptor open channel blockers / Chris G. Parsons and Kate E. Gilling; title: 4. State-of-the-art automated patch clamp: heat activation, action potentials, and high throughput in ion channel screening / Sonja Stoelzle-Feix; title: 5. Industrializing electrophysiology: HT automated patch clamp on SyncroPatch96 using instant frozen cells / Liudmila Polonchuk; title: 6. Planar patch clamp for neuronal networks - considerations and future perspectives / Alessandro Bosca, Marzia Martina, and Christophe Py; title: 7. Methods for the study of synaptic receptor functional properties / Enrica Maria Petrini and Andrea Barberis; title: 8. Single cell RT-PCR, a technique to decipher the electrical, anatomical, and genetic determinants of neuronal diversity / Maria Toledo-Rodriguez and Henry Markham; title: 9. Whole-cell voltage clamp on skeletal muscle fibers with the silicone-clamp technique / Romain Lefebvre, Sandrine Pouvreau, Claude Collet, Bruno Allard, and Vincent Jacquemond; title: 10. Multiphoton imaging approaches for studying striatal dendritic excitability / Joshua L. Plotkin and D. James Surmeier; title: 11. Investigation of synaptic microcircuits using patch-clamp paired recordings in acute brain slices / Giovanni Russo and Stefano taverna; title: 12. Outside-out "sniffer-patch" clamp technique for in situ measures of neurotransmitter release / Émilie Muller-Chrétien; title: 13. A cost-effective method for preparing, maintaining, and transfecting neurons in organotypic slices / Cary Soares, Kevin F.H. Lee, Denise Cook and Jean-Claude Béïque; title: 14. Acute brain slice methods for adult and aging animals: application of targeted patch clamp analysis and optogenetics / Jonathan T. Ting, Tanya L. Daigle, Qian Chen and Guoping Feng; title: 15. Synaptic connectivity in engineered neuronal networks / Peter Molnar, Jung-Fong Kang, Neelima Bhargava, Mainak Das and James J. Hickman; title: 16. Modeling of action potential generation in NG108-15 cells / Peter Molnar and James J. Hickman; title: 17. Whole-cell patch-clamp recordings in freely moving animals / Albert K. Lee, Jâerãome Epsztein and Michael Brecht; title: 18. Pressure-polished borosilicate pipettes are "universal sealer" yielding low access resistance and efficient intracellular perfusion / Marco Aquila, Mascia Benedusi, Anna Fasoli and Giorgio Rispoli; title: 19. Current recordings at the single channel level in adult mammalian isolated cardiomyocytes / Romain Guinamard, Thomas Hof, and Laurent Sallâe; title: 20. Dynamic clamp as a tool to study the functional effects of individual membrane currents / Géza Berecki, Arie O. Verkerk, Antoni C.G. van Ginneken and Ronald Wilders; title: 21. Dynamic clamp in cardiac and neuronal systems using RTXI / Francis A. Ortega, Robert J. Butera, David J. Christini, John A. White and Alan D. Dorval II; title: 22. Ion selectivity of pore-forming peptides and ion channels measured in xenopus oocytes / Thierry Cens and Pierre Charnet; title: 23. Principles of single-channel kinetic analysis / Feng Qin.