G protein-coupled receptors in drug discovery
By: edited by Wayne R. Leifert
Series: Methods in molecular biology -- 552; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 552.; Springer protocols
Publisher: Humana Press
Published: 2009
Place of publication: New York
Language: eng
Number of pages: 376
ISBN-13: 9781603273169; 9781603273176
Subjects: Recherche; Drug development; Receptors; Screening; Untersuchungsmethode; Wirkstoff; Arzneimittelforschung; Laboratory Manuals; Protéines G; Pharmacie; Récepteurs; Receptors, G-Protein-Coupled; G-Proteine; G proteins; Drug Discovery; Rezeptor
Notes: Includes bibliographical references and index.
Table of contents: title: New insights into GPCR function: implications for HTS  -- R.M. Eglen and T. Reisine; title: Screening technologies for G protein-coupled receptors: from HTS to uHTS  -- M. De los Frailes and E. Diez; title: GPCR signaling: understanding the pathway to successful drug discovery  -- C. Williams and S.J. Hill; title: An overview on GPCRs and drug discovery: structure-based drug design and structural biology on GPCRs  -- K. Lundstrom; title: Understanding the ligand-receptor-G protein ternary complex for GPCR drug discovery  -- V.R. Ratnala and B. Kobilka; title: Assay data quality assessment  -- H. Gubler; title: Homology modeling of GPCRs  -- J. Simms ... [et al.]; title: GPCR expression using baculovirus-infected Sf9 cells  -- A.L. Aloia ... [et al.]; title: Radioligand binding assays: application of [(125)I]angiotensin II receptor binding  -- W.R. Leifert ... [et al.]; title: [35S]GTPgammaS binding in G protein-coupled receptor assays  -- T. Cooper, E.J. McMurchie and W.R. Leifert; title: A time-resolved fluorescent lanthanide (Eu)-GTP binding assay for chemokine receptors as targets in drug discovery  -- J. Labrecque, R.S. Wong and S.P. Fricker; title: Use of the DiscoveRx Hit hunter cAMPII assay for direct measurement of cAMP in Gs and Gi GPCRs  -- J. Bradley and D. McLoughlin; title: Use of aequorin for G protein-coupled receptor hit identification and compound profiling  -- S.J. Brough and P. Shah; title: BacMam: versatile gene delivery technology for GPCR assays  -- E.A. Davenport, P. Nuthulaganti and R.S. Ames; title: Yeast assays for G protein-coupled receptors  -- S.J. Dowell and A.J. Brown; title: GPCR microspot assays on solid substrates  -- Y. Fang and J. Lahiri; title: Resonant waveguide grating biosensor for whole-cell GPCR assays  -- Y. Fang, A.M. Ferrie and E. Tran; title: FRET-based measurement of GPCR conformational changes  -- S. Granier ... [et al.]; title: FLIPR assays of intracellular calcium in GPCR drug discovery  -- K.B. Hansen and H. Brauner-Osborne; title: Use of fluorescence indicators in receptor ligands  -- K.G. Harikumar and L.J. Miller; title: Application of fluorescence resonance energy transfer techniques to establish ligand-receptor orientation  -- K.G. Harikumar and L.J. Miller; title: Detection of GPCR/beta-arrestin interactions in live cells using bioluminescence resonance energy transfer technology  -- M. Kocan and K.D. Pfleger; title: Using reporter gene technologies to detect changes in cAMP as a result of GPCR activation  -- D.J. Rodrigues and D. McLoughlin; title: A quantum dot-labeled ligand-receptor binding assay for G protein-coupled receptors contained in minimally purified membrane nanopatches  -- J.L. Swift, M.C. Burger and D.T. Cramb; title: Xenopus oocyte electrophysiology in GPCR drug discovery  -- K.B. Hansen and H. Brauner-Osborne; title: Immunoprecipitation and phosphorylation of G protein-coupled receptors -- W.G. Thomas.