Ethanol production in actinomycetes after expression of synthetic adhB and pdc
Contributors: Luzhetska, Marta Verfasser; Ebeling, Sandra 1984- Verfasser; Bechthold, Andreas 1962- Verfasser; Albert-Ludwigs-Universität Freiburg Fakultät für Chemie, Pharmazie und Geowissenschaften; Albert-Ludwigs-Universität Freiburg Pharmazeutische Biologie und Biotechnologie; Albert-Ludwigs-Universität Freiburg
Publisher: Universität
Published: 2012
Place of publication: Freiburg
Language: eng
Subjects: Ethanolproduktion; Actinomycetaceae; Streptomyces saraceticus; Alkoholdehydrogenasen; Pyruvatdecarboxylase; Arzneimittelentwicklung; Arzneimitteldesign
Description: Abstract: Streptomyces strains are producing many important secondary metabolites, in many cases used as pharmaceuti-cal drugs. Though the usage of these bacteria for the production of fuel has never been described. We could show, that the expression of two artificial ethanologenic genes of Zymomonas mobilis in different actinomycetes strains resulted in the production of ethanol. The synthetic genes adhB and pdc encoding an alcohol dehydrogenase and a pyruvate decarboxy-lase, respectively, were expressed in eight different Streptomyces strains. Best production was obtained using Streptomy-ces coelicolor A3(2) harboring both genes. By variation of the cultivation conditions, the amount of ethanol produced by the strain could be increased up to 2.6 g/l
Notes: The open biotechnology journal. 6 (2012), 13-16, DOI 10.2174/1874070701206010013, issn: 1874-0707

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