2D PAGE
Subtitle: sample preparation and fractionation
By: edited by Anton Posch.
Contributors: Posch, Anton.
Series: Methods in molecular biology -- v. 424-425; Methods in molecular biology (Clifton, N.J.) -- v. 424-425.
Publisher: Humana Press
Published: 2008
Place of publication: Totowa, NJ
Language: eng
ISBN-13: 9781603272094; 9781588297228; 9781603270649
Subjects: Proteomics; Two-dimensional electrophoresis; Proteomics -- methods; Electrophoresis, Gel, Two-Dimensional -- methods
Other titles: Two-dimensional PAGE sample preparation and fractionation
Notes: Includes bilbiographical references and indexes.
Table of contents: title: [Volume 1. focuses on the basics of sample preparation and protein labeling techniques with an emphasis on fractionation by chemical reagents, chromatography, and electrophoresis methods.  The inclusion of meticulous enrichment further aids the protein researcher.]; title: Volume 2. Application of fluorescence dye saturation labeling for differential proteome analysis of 1,000 microdissected cells from pancreatic ductal adenocarcinoma precursoe lesions / Barbara Sitek ... [et al.]; title: Albumin and immunoglobulin depletion of human plasma / Rosalind e. Jenkins ... [et al.]; title: Multi-component immunoaffinity subtraction and reversed-phase chromatography of human serum / James Martosella and Nina Zolotarjova; title: Immunoaffinity fractionation of plasma proteins by chicken IgY antibodies / Lei Huang and Xiangming Fang; title: Proteomics of cerebrospinal fluid methods for sample processing / John E. Hale ... [et al.]; title: Sample preparation of bronchoalveolar lavage fluid / Baptiste Leroy, Paul Falmagne, and Ruddy Wattiez; title: Preparation of nasal secretions for peoteome analysis / Begona Casado, Paolo Iadarola, and Lewis K. Pannell; title: Preparation of urine samples for proteomic analysis / Rembert Pieper; title: Isolation of cytoplasmatic proteins from cultures cells for two-dimensional gel electrophoresis / Ying Wang, Jen-Fu Chiu, and Qing-Yu He; title: Sample preparation of culture medium from Madin-Darby Canine Kidney cells / Daniel Ambort, Daniel Lottaz, and Erwin Sterchi; title: Sample preparation for mass spectromentry analysis of formalin-fixed paraffin-embedded tissue proteomic analysis of formalin-fixed tissue / Nicolas A. Stewart and Timothy D. Veenstra; title: Metalloproteomics in the molecular study of cell physiology and disease / Hermann-Josef Thierse, Stefanie Helm, and Patrick Pankert; title: Protein extraction from green plant tissue / Ragnar Flengsrud; title: The terminator a device for high-throughput extraction of plant material / B. M. van den Berg; title: Isolation of mitochondria from plant cell culture / Etienne H. Meyer and A. Harvey Millar; title: Isolation and preparation of choroplasts from Arabidopsis thaliana plants / Sybille E. Kubis, Kathryn S. Lilley, and Paul Jarvis; title: Isolation of plant cell wall proteins / Elisabeth Jamet ... [et al.]; title: Isolation and fractionation of the endoplasmic reticulum from castor bean (Ricinius communis) endosperm for proteomic analyses / William J. Simon, Daniel J. Maltman, and Antoni R. Slabas; title: Cell wall fractionation for yeast and fungal proteomics / Aida Pitarch, Césa Nombela, and Concha Gil; title: Sample preparation procedure for cellular fungi / Alois Harder; title: Isolation and enrichment of secreted proteins from filamentous fungi / Martha L. Medina and Wilson A. Frnacisco; title: Isolation and solubilization of cellular membrane proteins from bacteria / Kheir Zuobi-Hasona and L. Jeannine Brady; title: Isolation and solubilization of gram-positive bacterial cell wall-associated proteins / Jason N. Cole, Steven P. Djordjevic, and Mark J. Walker; title: Cell fractionation of parasitic protozoa / Wanderley de Souza, José Andrés-Morgado-Diaz, and Narcisa L. Cunha-e-Silva