Therapeutic applications of ribozymes and riboswitches
Subtitle: methods and protocols
By: edited by Daniel Lafontaine and Audrey Dubé, Department of Biology, Université de Sherbrooke, Sherbrooke, QC, Canada
Series: Methods in molecular biology -- 1103; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 1103.; Springer protocols (Series)
Publisher: Humana Press
Published: 2014
Place of publication: New York
Language: eng
Number of pages: 278
ISBN-13: 9781627037297
Copyright date: 2014
Subjects: Catalytic RNA; Laboratory manuals; Riboswitches; Laboratory Manuals; Therapeutic use; Riboswitch; Ribozym; Therapie
Notes: Includes bibliographical references and index.
Table of contents: title: Identification of regulatory RNA in bacterial genomes by genome-scale mapping of transcription start sites --; title: Screening inhibitory potential of anti-HIV RT RNA aptamers --; title: Design and evaluation of clinically relevant SOFA-HDV ribozymes targeting HIV RNA --; title: Directing RNase P-mediated cleavage of target mRNAs by engineered external guide sequences in cultured cells --; title: Design and analysis of hammerhead ribozyme activity against an artificial gene target --; title: Knockdown strategies for the study of proprotein convertases and proliferation in prostate cancer cells --; title: Use of tumor-targeting trans-splicing ribozyme for cancer treatment --; title: Characterization of hairpin ribozyme reactions --; title: Finding instances of riboswitches and ribozymes by homology search of structured RNA with infernal --; title: Structure-based virtual screening for the identification of RNA- binding ligands --; title: Probing riboswitch binding sites with molecular docking, focused libraries, and in-line probing assays --; title: Discovery of small molecule modifiers of micrornas for the treatment of HCV infection --; title: Bacterial flavin mononucleotide riboswitches as targets for flavin analogs --; title: Construction and application of riboswitch-based sensors that detect metabolites within bacterial cells --; title: Screening assays to identify artificial glmS ribozyme activators --; title: Analysis of riboswitch structure and ligand binding using small-angle X-ray scattering (SAXS) --; title: Use of SHAPE to select 2AP substitution sites for RNA-ligand interactions and dynamics studies --; title: Cell internalization SELEX: In vitro selection for molecules that internalize into cells --; title: DNA electronic switches based on analyte-responsive aptamers.