Phosphoinositide-3-kinases p110α and p110β mediate S phase entry in astroglial cells in the marginal zone of rat neocortex
Contributors: Fischer, Catharina 1977- Verfasser; Wilmes, Thomas 1979- Verfasser; Heimrich, Bernd 1956- Verfasser; Distel, Vanessa 1981- Verfasser; Klugbauer, Axel Verfasser; Meyer, Dieter K. Verfasser; Albert-Ludwigs-Universität Freiburg Institut für Experimentelle und Klinische Pharmakologie und Toxikologie; Albert-Ludwigs-Universität Freiburg Abteilung für Neuroanatomie; Albert-Ludwigs-Universität Freiburg Medizinische Fakultät; Albert-Ludwigs-Universität Freiburg
Publisher: Universität
Published: 2013
Place of publication: Freiburg
Language: eng
Subjects: Bromdesoxyuridin; Glycogen-Synthase-Kinase-3; Peripherie; Neocortex; Ratte; Nichtmedikamentöse Therapie
Description: Abstract: In cells cultured from neocortex of newborn rats, phosphoinositide-3-kinases of class I regulate the DNA synthesis in a subgroup of astroglial cells. We have studied the location of these cells as well as the kinase isoforms which facilitate the S phase entry. Using dominant negative (dn) isoforms as well as selective pharmacological inhibitors we quantified S phase entry by nuclear labeling with bromodeoxyuridine (BrdU). Only in astroglial cells harvested from the marginal zone (MZ) of the neocortex inhibition of phosphoinositide-3-kinases reduced the nuclear labeling with BrdU, indicating that neocortical astroglial cells differ in the regulation of proliferation. The two kinase isoforms p110α and p110β were essential for S phase entry. p110α diminished the level of the p27Kip1 which inactivates the complex of cyclin E and CDK2 necessary for entry into the S phase. p110β phosphorylated and inhibited glycogen synthase kinase-3β which can prevent S-phase entry. Taken together, both isoforms mediated S phase in a subgroup of neocortical astroglial cells and acted via distinct pathways
Notes: Frontiers in cellular neuroscience. 7 (2013), 24, DOI 10.3389/fncel.2013.00024, issn: 1662-5102

IN COPYRIGHT http://rightsstatements.org/page/InC/1.0 rs

Archivierung/Langzeitarchivierung gewährleistet