RNA turnover in eukaryotes
Subtitle: nucleases, pathways and analysis of mRNA decay
By: edited by Lynne E. Maquat, Megerditch Kiledjian
Contributors: ScienceDirect (Online service)
Series: Methods in enzymology -- v. 448
Publisher: Academic
Published: 2008
Place of publication: San Diego, Calif; London
Language: eng
Format: [electronic resource] :
Number of pages: 607
ISBN-13: 9780080922072; 9780123743787
Subjects: Nucleases; Molecular Biology; Genetic regulation; SCIENCE; Eukaryotic cells; Messenger RNA; Metabolism; RNA Stability; Life Sciences; Eukaryotic Cells; RNA Precursors; MRNA Cleavage and Polyadenylation Factors; Laboratory Manuals; Physiology
Notes: Includes bibliographical references and indexes.

Description based on print version record.
Table of contents: title: Analysis of mRNA decapping / S.W. Liu ... [et al.]; title: A kinetic assay to monitor RNA decapping under single- turnover conditions / B.N. Jones ... [et al.]; title: Purification and analysis of the decapping activator Lsm1p-7p-Pat1p complex from yeast / S. Tharun; title: Reconstitution of recombinant human LSm complexes for biochemical, biophysical, and cell biological studies / B.L. Zaric and C. Kambach; title: Regulated deadenylation in vitro / A.C. Goldstrohm, B.A. Hook and M. Wickens; title: Cell-free deadenylation assays with Drosophila embryo extracts / M. Jeske and E. Wahle; title: Measuring CPEB-mediated cytoplasmic polyadenylation-deadenylation in Xenopus laevis oocytes and egg extracts / J.H. Kim and J.D. Richter; title: The preparation and applications of cytoplasmic extracts from mammalian cells for studying aspects of mRNA decay / K.J. Sokoloski, J. Wilusz and C.J. Wilusz; title: In vitro assays of 5' to 3'-exoribonuclease activity / O. Pellegrini ... [et al.]; title: ^; title: Reconstitution of RNA exosomes from human and Saccharomyces cerevisiae cloning, expression, purification, and activity assays / J.C. Greimann and C.D. Lima; title: Biochemical studies of the mammalian exosome with intact cells / G. Schilders and G.J. Pruijn; title: Determining in vivo activity of the yeast cytoplasmic exosome / D. Schaeffer ... [et al.]; title: Approaches for studying PMR1 endonuclease-mediated mRNA decay / Y. Otsuka and D.R. Schoenberg; title: Methods to determine mRNA half-life in Saccharomyces cerevisiae / J. Coller; title: mRNA Decay analysis in Drosophila melanogaster drug-induced changes in glutathione S-transferase D21 mRNA stability / B. Akgul and C.P. Tu; title: Measuring mRNA stability during early Drosophila embryogenesis / J.L. Semotok ... [et al.]; title: Messenger RNA half-life measurements in mammalian cells / C.Y. Chen, N. Ezzeddine and A.B. Shyu; title: Trypanosomes as a model to investigate mRNA decay pathways / S. Archer ... [et al.]; title: ^; title: ^^; title: Cell type-specific analysis of mRNA synthesis and decay in vivo with uracil phosphoribosyltransferase and 4-thiouracil / M.D. Cleary; title: Analysis of cytoplasmic mRNA decay in Saccharomyces cerevisiae / D.O. Passos and R. Parker; title: Transcriptome targets of the exosome complex in plants / D. Belostotsky; title: Sensitive detection of mRNA decay products by use of reverse-ligation-mediated PCR (RL-PCR) / T. Grange; title: Tethering assays to investigate nonsense-mediated mRNA decay activating proteins / N.H. Gehring, M.W. Hentze and A.E. Kulozik; title: Assays for determining poly(A) tail length and the polarity of mRNA decay in mammalian cells / E.L. Murray and D.R. Schoenberg; title: Analyzing P-bodies in Saccharomyces cerevisiae / T. Nissan and R. Parker; title: Real-time and quantitative imaging of mammalian stress granules and processing bodies / N. Kedersha ... [et al.]; title: Cell biology of mRNA decay / D. Grunwald, R.H. Singer and K. Czaplinski.; title: ^^