Molecular cloning, the condensed protocols
Subtitle: a laboratory manual
By: Joseph Sambrook, David W. Russell ; associate authors, Nina Irwin, Kaaren A. Janssen.
Contributors: Russell, David W. 1957-; Irwin, Carleen Ann.; Janssen, Kaaren A.
Publisher: Cold Spring Harbor Laboratory Press
Published: 2006
Place of publication: Cold Spring Harbor, N.Y
Language: eng
Number of pages: 800
Subjects: Molecular cloning -- Laboratory manuals; Cloning, Molecular -- Laboratory Manuals
Genres: Laboratory manuals.; Laboratory Manuals.
Notes: Includes index.
Table of contents: title: Plasmids and their usefulness in molecular cloning; title: Bacteriophage and its vectors; title: Working with bacteriophage M13; title: Working with high-capacity vectors; title: Gas electrophoresisof DNA and pulsed-field agarose; title: Preparation and analysis of eukaryotic genomic DNA; title: Extraction, purification, and analysis of mRNA from eukaryotic cells; title: In vitro amplification of DNA by the polymerase chain reaction; title: Preparation of radiolabeled DNA and RNA probes; title: Working with synthetic oligonucleotide probes; title: Preparation of cDNA libraries and gene identification; title: DNA sequencing; title: Mutagenesis; title: Screening expression libraries; title: Expression of cloned genes in escherichia coli; title: Introducing cloned genes into cultured mammalian cells; title: Analysis of gene expression in cultured mammalian cells; title: Protein interaction technologies.