Human cell culture protocols.
Contributors: Picot, Joanna.
Edition: 2nd ed. / edited by Joanna Picot.
Series: Methods in molecular medicine -- 107
Publisher: Humana Press
Published: 2005
Place of publication: Totowa, N.J
Language: eng
Number of pages: 359
Subjects: Human cell culture -- Laboratory manuals.; Cells, Cultured -- Laboratory Manuals.; Cytological Techniques -- Laboratory Manuals.
Genres: Laboratory manuals.; Laboratory Manuals.
Description: Revised and updated collection of readily reproducible techniques for culturing human cells. This new edition includes a wide range of human cell types relevant to human disease and new chapters on fibroblasts, Schwann cells, gastric and colonic epithelial cells, and parathyroid cells. The protocols follow the series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Notes: Includes bibliographical references and index.
Table of contents: title: Establishment and maintenance of normal human keratinocyte cultures / Claire Linge; title: Cultivation of normal human epidermal melanocytes in the absence of phorbol esters / Mei-Yu Hsu, Ling Li, and Meenhard Herlyn; title: Isolation and culture of human osteoblasts / Alison Gartland ... [et al.]; title: Human osteoclast culture from peripheral blood monocytes : phenotypic characterization and quantitation of resorption / Katherine A. Buckley ... [et al.]; title: Human chondrocyte cultures as models of cartilage-specific gene regulation / Mary B. Goldring; title: Human myoblasts and muscle-derived SP cells / Grace K. Pavlath and Emanuela Gussons; title: Cell cultures of autopsy-derived fibroblasts / Volker Meske, Frank Albert, and Thomas G. Ohm; title: Culture and differentiation of human adipocyte precursor cells in primary culture / Vanessa van Harmelen, Thomas Skurk, and Hans Hauner; title: Human mononuclear phagocytes in tissue culture / Yona Keisari; title: Purification of peripheral blood natural killer cells / Bice Perussia and Matthew J. Loza; title: Human fetal brain cell culture / Mark P. Mattson; title: Culturing human schwann cells / Victor J. Turnbull; title: Well-differentiated human airway epithelial cell cultures / M. Leslie Fulcher ... [et al.]; title: Isolation and culture of human alveolar epithelial cells / Carsten Ehrhardt, Kwang-Jin Kim, and Claus-Michael Lehr; title: A new approach to primary culture of human gastric epithelium / Pierre Chailler and Daniel MÄnard; title: Isolation and culture of human colon epithelial cells using a modified explant technique employing a noninjurious approach / Hamid A. Mohammadpour; title: Isolation and culture of human hepatocytes / Martin Bayliss and Graham Somers; title: Glomerular epithelial and mesangial cell culture and characterization / Heather M. Wilson and Keith N. Stewart; title: Isolation and culture of human renal cortical cells with characteristics of proximal tubules / Gabrielle M. Hawksworth; title: Culture of parathyroid cells / Per Hellman; title: Long-term culture and maintenance of human islets of langerhans in memphis serum-free media / Daniel W. Fraga; title: Primary culture of human antral endocrine and epithelial cells / Susan B. Curtis and Alison M. J. Buchan; title: Conjunctiva organ and cell culture / Monica Berry and Marcus Radburn-Smith; title: Establishment and maintenance of in vitro cultures of human retinal pigment epithelium / Martin J. Stevens.