Plant proteomics
Subtitle: methods and protocols
By: edited by Jesus V. Jorrin-Novo, Department of Biochemistry and Molecular Biology, University of Cordoba, Cordoba, Spain, Setsuko Komatsu, National Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba, Japan, Wolfram Weckwerth and Stefanie WienKoop, Department of Ecogenomics and Systems Biology, University of Vienna, Vienna, Austria
Edition: Second edition.
Series: Methods in molecular biology -- 1072
Publisher: Humana Press
Published: 2014
Place of publication: New York
Language: eng
Number of pages: 786
ISBN-13: 9781627036306; 9781627036313
Subjects: Plant proteins; Plant proteomics
Description: Plant Proteomics: Methods and Protocols, Second Edition presents recent advances made in the field of proteomics and their application to plant biology and translational research. In recent years, improvements in techniques and protocols for high-throughput proteomics have been made at all workflow stages, from wet (sampling, tissue and cell fractionation, protein extraction, depletion, purification, separation, MS analysis, quantification) to dry lab (experimental design, algorithms for protein identification, bioinformatics tools for data analysis, databases, and repositories). Divided into nine convenient sections, chapters cover topics such as applications of gel-free, label- or label-free, imaging and targeted approaches to experimental model systems, crops and orphan species, as well as the study and analysis of PTMs, protein interactions, and specific families of proteins, and finally proteomics in translational research. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Notes: Revision of: Plant proteomics / edited by Hervé Thiellement ... [et al.]. 2007.

Includes bibliographical references and index.