Partial least squares modeling of keratinocyte differentiation through the integration of gene expression profiles and flow cytometry analysis.
Published: 2007
Language: eng
Number of pages: 228
ISBN-13: 9780494272725
Description: The use of keratinocytes in wound dressings for the treatment of chronic wounds is a viable treatment option, although limitations of this application arise when keratinocytes differentiate during initial expansion. This work involved the generation of partial least squares and multi-way partial least squares models to investigate gene expression events associated with keratinocyte differentiation. A heterogeneous keratinocyte population was characterized using multi-parameter flow cytometry and enriched using Percoll gradient separation. The two modeling approaches were used to took at gene expression events over time in conjunction with flow cytometry measurements of differentiation. Multi-way models were able to simplify analysis and provide a robust way to capture time course events. The models were able to focus on a set of common genes positively and negatively associated with differentiation, including members of the Notch signaling pathway. This study established models of differentiation and provided a basis for future model development.
Notes: Source: Masters Abstracts International, Volume: 45-06, page: 3158.

Thesis (M.A.Sc.)--University of Toronto, 2007.

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