Mesenchymal stem cells
Subtitle: methods and protocols
By: edited by Darwin J. Prockop, Donald G. Phinney, Bruce A. Bunnell.
Contributors: Prockop, Darwin J.; Phinney, Donald G.; Bunnell, Bruce A.
Series: Methods in molecular biology -- 449; Methods in molecular biology (Clifton, N.J.) -- v. 449.
Publisher: Humana Press
Published: 2008
Place of publication: Totowa, NJ
Language: eng
Number of pages: 192
ISBN-13: 9781588297716
Subjects: Mesenchymal Stem Cells -- cytology; Cell Culture Techniques; Cells, Cultured; Cytological Techniques; Multipotent Stem Cells -- cytology; Stromal Cells -- cytology; Stem cells -- Laboratory manuals
Genres: Laboratory manuals.
Notes: Includes bibliographical references and index.
Table of contents: title: Part I. Isolation of human multipotential stromal cells from bone marrow and adipose tissue.  Isolation and culture of bone marrow-derived human multipotent stromal cells (hMSCs) / Margaret Wolfe ... [et al.].  Mesenchymal stem cells from adult bone marrow / Mark F. Pittenger.  A method to isolate and purify human bone marrow stromal stem cells / Stan Gronthos and Andrew C. W. Zannettino.  Adipose-derived stem cells / John K. Fraser ... [et al.].  Isolation of human adipose-derived stem cells from biopsies and liposuction specimens / Severine G. Dubois ... [et al.]; title: Part II. Manipulation of human multipotential stromal cells.  Colony forming unit assays for MSCs / Radhika Pochampally.  Differentiation and characterization of human MSCs / Roxanne L. Reger, Alan H. Tucker, and Margaret R. Wolfe.  Freezing harvested hMSCs and recovery of hMSCs from frozen vials for subsequent expansion, analysis, and experimentation / Roxanne L. Reger and Margaret R. Wolfe.  Gene expression analysis at the single cell level using the human bone marrow stromal cell as a model : sample preparation methods / Beerelli Seshi.  Assays of MSCs with microarrays / Joni Yöstalo, Radhika Pochampally, and Darwin J. Prockop.  Gene delivery to mesenchymal stem cells / Reza Izadpanah and Bruce A. Bunnell; title: Part III. Isolation and characterization of murine multipotential stromal cells from bone marrow.  Isolation of mesenchymal stem cells from murine bone marrow by immunodepletion / Donald G. Phinney.