Basic methods in protein purification and analysis
Subtitle: a laboratory manual
By: edited by Richard J. Simpson, Peter D. Adams, Erica A. Golemis.
Contributors: Simpson, Richard J.; Adams, Peter D.; Golemis, Erica.
Publisher: Cold Spring Harbor Laboratory Press
Published: 2008
Place of publication: Cold Spring Harbor, N.Y
Language: eng
ISBN-13: 9780879698683; 9780879698676
Subjects: Proteins -- Purification -- Laboratory manuals; Proteins -- Analysis -- Laboratory manuals; Proteins -- isolation & purification -- Laboratory Manuals; Chromatography -- methods -- Laboratory Manuals; Electrophoresis -- methods -- Laboratory Manuals; Immunoprecipitation -- methods -- Laboratory Manuals; Proteins -- analysis -- Laboratory Manuals
Genres: Laboratory manuals; Laboratory Manuals
Notes: Includes bibliographical references and index.
Table of contents: title: Introduction to protein purification strategies; title: Preparation of cellular and subcellular extracts; title: One-dimensional polyacrylamide gel electrophoresis; title: Preparative two-dimensional gel electrophoresis with immobilized PH gradients; title: Ion-exchange chromatography; title: Size-exclusion chromatography; title: Reversed-phase high-performance liquid chromatography; title: Affinity and immunoaffinity chromatography; title: Metal chelate affinity chromatography; title: Multidimensional chromatography of intact proteins; title: Identification of protein-protein interactions by conventional column chromatography; title: Identification of associated proteins by coimmunoprecipitation; title: Chromatin immunoprecipitation of protein complexes; title: Identification of protein-protein interactions with glutathione-s-transferase fusion proteins; title: Direct visualization of protein interactions in living cells using bimolecular fluorescence complementation analysis; title: In silico tools to launch protein interaction analysis : creating focused networks around proteins of interest.