Nanoproteomics
Subtitle: methods and protocols
By: edited by Steven A. Toms, Robert J. Weil
Series: Methods in molecular biology -- v. 790
Publisher: Springer; Humana
Published: 2011
Place of publication: New York
Language: eng
Number of pages: 322
ISBN-13: 9781617793189; 9781617793196
Subjects: Proteomics; Nanotechnology
Description: As two relatively new fields of study, proteomics and nanotechnology have developed in parallel with each other to allow an increased precision in the identification of post-translational protein modifications as well as to provide a more automated isolation and detection of rare proteins in both serum and tissues. The Nanoproteomics: Methods and Protocols volume organizes and collects technical advances from leaders in the field to make laboratory protocols more readily available and understandable to those who are attempting to incorporate nanotechnologic techniques into their proteomic research. Conveniently divided into five sections, this detailed volume covers preliminary sample preparation, nanoscale fluidic devices and methods, nanostructured surfaces and nanomaterials, and nanoproteomic techniques to detect and understand protein and proteomic alterations specific to human pathology. Written in the highly successful series entitled Methods in Molecular Biology, these chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols that are readily reproducible, and tips on troubleshooting and avoiding known pitfalls. Convenient and authoritative, Nanoproteomics: Methods and Protocols offers key procedures that are culled from the laboratories of leaders in the field of nanoproteomics with the aim of helping researchers in their standardization and proliferation of protocols that will lead to a more wide scale adoption and smoother progress in this vital field.
Notes: Includes bibliographical references and index.
Table of contents: title: MALDI-TOF serum profiling using semiautomated serum peptide capture with magnetic reversed phase (C18) beads  -- Jaco C. Knol and Connie R. Jimenez; title: Proteomics of epithelial lining fluid obtained by bronchoscopic microprobe sampling  -- Lorenza Franciosi ... [et al.]; title: Protein identification using nano-HPLC-MS : ESI-MS and MALDI-MS interfaces  -- Rui Vitorino ... [et al.]; title: Three-dimensional peptide fractionation for highly sensitive nanoscale LC-based shotgun proteomic analysis of complex protein mixtures  -- Sricharan Bandhakavi ... [et al.]; title: Nanospray ion mobility mass spectrometry of selected high mass species  -- Iain Campuzano and Kevin Giles; title: Nanoelectrospray-MSn of native and permethylated glycans  -- Christina Bleckmann, Hildegard Geyer, and Rudolf Geyer; title: N-linked global glycan profiling by nanoLC mass spectrometry -- Michael S. Bereman and David C. Muddiman; title: ^; title: Applications of nanoscale liquid chromatography coupled to tandem mass spectrometry in quantitative studies of protein expression protein-protein interaction, and protein phosphorylation  -- Metodi V. Metodiev; title: Nano LC-MS/MS : a robust setup for proteomic analysis  -- Marco Gaspari and Giovanni Cuda; title: Nanofluidic devices for rapid continuous-flow bioseparation  -- Pan Mao and Jianping Fu; title: Quantifying attomole amounts of proteins from complex samples by nano-LC and selected reaction monitoring  -- Thomas Frohlich and Georg J. Arnold; title: Sample preparation method for gold nanoparticle-assisted laser desorption/ionization time-of-flight mass spectrometry  -- Yen-Hsiu Lin and Wei-Lung Tseng; title: Nanostructured TiO2 thin films for phosphoproteomics studies with MALDI mass spectrometry  -- Federico Torta ... [et al.]; title: Nanofilament silicon for matrix-free laser desorption/ionization mass spectrometry -- Chia-Wen Tsao and Don L. DeVoe; title: ^; title: Protein nanoarrays for high-resolution patterning of bacteria on gold surfaces  -- Cait Costello ... [et al.]; title: Engineered multifunctional nanotools for biological applications  -- Mohammed Ibrahim Shukoor ... [et al.]; title: Selective capture of phosphopeptides by zirconium phosphonate-magnetic nanoparticles  -- Liang Zhao, Renan Wu, and Hanfa Zou; title: Nanowire biosensors for label-free, real-time, ultrasensitive protein detection  -- Gengfeng Zheng and Charles M. Lieber; title: Generation of anti-infectome/anti-proteome nanobodies  -- Gholamreza Hassanzadeh-Ghassabeh, Dirk Saerens, and Serge Muyldermans; title: Isolation, propagation, and analysis of biological nanoparticles  -- Michael P. Linnes ... [et al.]; title: Functionalized soluble nanopolymers for phosphoproteome analysis  -- Anton Iliuk ... [et al.]; title: Elucidating structural dynamics of integral membrane proteins on native cell surface by hydroxyl radical footprinting and nano LC-MS/MS ; title: ^ -- Yi Zhu, Tiannan Guo, and Siu Kwan Sze; title: Application of electrostatic repulsion hydrophilic interaction chromatography to the characterization of proteome, glycoproteome, and phosphoproteome using nano LC-MS/MS -- Piliang Hao, Huoming Zhang, and Siu Kwan Sze.