Cryo-EM
Subtitle: Sample preparation and data collection
By: edited by Grant J. Jensen
Contributors: ScienceDirect (Online service)
Series: Methods in enzymology -- v. 481
Publisher: Academic Press/Elsevier
Published: 2010
Place of publication: San Diego, Calif
Language: eng
Format: [electronic resource] /
ISBN-13: 9780123749062; 9780080956954
Subjects: Electron microscopy; Technique; Proteins; Structure; Genomics
Notes: Title from PDF title page (ScienceDirect, viewed Oct. 4, 2010).

Includes bibliographical references and indexes.
Table of contents: title: Part A: Sample preparation and data collection. 3d reconstruction from electron micrographs a personal account of its development; title: Preparation of 2d crystals of membrane proteins for high-resolution electron crystallography data collection; title: Helical crystallization of soluble and membrane binding proteins; title: Plunge freezing for electron cryomicroscopy; title: A practical guide to the use of monolayer purification and affinity grids; title: Grafix: Stabilization of fragile macromolecular complexes for single particle cryo-EM; title: Cryonegative staining of macromolecular assemblies; title: Liposomes on a streptavidin crystal: A system to study membrane proteins by cryo-EM; title: Micromanipulator-assisted vitreous cryosectioning and sample preparation by high-pressure freezing; title: Site-specific biomolecule labeling with gold clusters; title: How to operate a cryo-electron microscope; title: Collecting electron crystallographic data of two-dimensional protein crystals; title: Automated data collection for electron microscopic tomography; title: Correlated light and electron cryo-microscopy; title: Phase plates for transmission electron microscopy; title: Radiation damage in electron cryomicroscopy.