Agrobacterium protocols
By: edited by Kan Wang.
Contributors: Wang, Kan, 1957-
Edition: 2nd ed.
Series: Methods in molecular biology ;; 343; Methods in molecular biology (Clifton, N.J.) ;; v. 343.
Publisher: Humana Press
Published: 2006
Place of publication: Totowa, N.J
Language: eng
Subjects: Agrobacterium; Agricultural biotechnology; Rhizobium radiobacter -- genetics -- Laboratory Manuals; Rhizobium radiobacter -- isolation & purification -- Laboratory Manuals; Bacteriological Techniques -- methods -- Laboratory Manuals; Genetic Techniques -- Laboratory Manuals
Genres: Laboratory Manuals.
Description: "A gold standard collection of Agrobacterium-mediated transformation techniques for state-of-the-art plant genetic engineering, functional genomic analysis, and crop improvement. Volume 1 details the most updated techniques available for twenty-six plant species drawn from cereal crops, industrial plants, legume plants, and vegetable plants, and presents various methods for introducing DNA into three major model plant species, Arabidopsis thaliana, Medicago truncatula, and Nicotiana. The authors also outline the basic methods in Agrobacterium manipulation and strategies for vector construction. Volume 2 contains another thirty-three proven techniques for root plants, turf grasses, woody species, tropic plants, nuts and fruits, ornamental plants, and medicinal plants. Additional chapters provide methods for introducing DNA into non-plant species, such as bacteria, fungi, algae, and mammalian cells. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls."--Publisher's website.
Notes: Includes bibliographical references and index.