The Enzymes
Subtitle: Nucleic acids
Edition: 3rd ed.
Publisher: Elsevier
Published: 1981
Place of publication: Oxford
Language: eng
Format: [electronic resource] :
Number of pages: 675
ISBN-13: 9780080865911
Subjects: Enzymes; Epidemiology; Nucleic acids; Epidemiologic methods; Enzymes -- Periodicals
Notes: Description based upon print version of record.

II. Purification and Chemical Properties

The Enzymes.
Table of contents: title: Front Cover; The Enzymes: Nucleic Acids; Copyright Page; Contents; List of Contributors; Preface; Section I. Ligases; Chapter 1. DNA Ligases; I. Introduction; II. Isolation and Physical Properties; III. Catalytic Properties; IV. Role of DNA Ligases in Vivo; V. Research Applications; Chapter 2. T4 RNA Ligase; I. Introduction; II. Purification and Properties; III. Reactions Catalyzed; IV. Biological Role; V. Applications; Section II. RNA Polymerares and Related Enzymes; Chapter 3. Bacterial DNA-Dependent RNA Polymerases; I. Background; II. Molecular Properties; III. Catalytic Properties; title: Chapter 4. Bacteriophage DNA-Dependent RNA PolymerasesI. Introduction; II. T7-Like RNA Polymerases; III. Other Bacteriophage RNA Polymerases; Chapter 5. Eukaryotic RNA Polymerases; I. Introduction; II. Purification; III. Subunit Structures; IV. Subunit Functions; V. Stimulatory Factors; VI. DNA Binding and Catalytic Properties of Purified RNA Polymerases; VII. Inhibitors; VIII. Eukaryotic Transcription Extract Systems; IX. Organelle- and Viral-Coded RNA Polymerases; Chapter 6. Priming Enzymes; I. Introduction; II. dnaG Gene Product from Escherichia coli; title: III. Multiple Pathways for dnaG Priming in SS to RF DNA ReplicationIV. Studies on the Specificity of dnaG-Template Interactions; V. Priming by RNA Polymerases; VI. Priming on Double-Stranded DNA; VII. Phage-Encoded Priming Enzymes; VIII. Priming in Eukaryotic Systems; Chapter 7. tRNA Nucleotidyltransferase; I. Introduction; II. Purification and Structural Studies; III. Catalytic Properties; IV. Biological Role; V. Research Applications; Chapter 8. Poly(A) Adding Enzymes; I. Introduction and Perspective; II. Purification and Properties; III. Multiple Poly(A) Polymerases; title: IV. Properties of Poly(A) Polymerase ProteinsV. Assay; VI. Stoichiometry; VII. Substrates; VIII. Primer Requirement; IX. Ion Requirements; X. Inhibitors; XI. Kinetics and Reaction Mechanism; XII. Biological Role; XIII. Regulation of Poly(A) Polymerases; XIV. Research Applications; Chapter 9. Capping Enzyme; I. Introduction; II. Vaccinia Virus Capping Enzymes; III. HeLa Cell Capping Enzymes; IV. Capping Enzyme from Rat Liver Nuclei; V. Role of the Capping Enzyme System in Vivo; VI. Research Applications of Vaccinia Virus Capping Enzyme; Chapter 10. Q© Replicase; I. Introduction; title: II. Purification and PropertiesIII. Catalytic Properties; Chapter 11. 2',5'-Oligoadenylate Synthetase; I. Introduction; II. Purification and Properties; III. Reactions Catalyzed; IV. Biological Role; Section III. RNA Nucleases and Related Enzymes; Chapter 12. Pancreatic Ribonuclease; I. Introduction; II. Preparation; III. Chemical Properties; IV. Physical Properties; V. Species Variations; VI. Bovine Seminal Plasma RNase; VII. Cytoplasmic RNase Inhibitor; VIII. Catalytic Properties; IX. Research Applications; Chapter 13. Ribonuclease T1; I. Introduction