Chromatin remodeling is implicated in negative regulation of the Caenorhabditis elegans her-1 gene during sex determination.
Published: 2004
Language: eng
Number of pages: 120
Description: The nematode C. elegans has two sexual fates: hermaphrodites and males. Both sexual fates are governed by a series of genes that fall into a negative regulatory pathway. The male fate is specified by her-1 , a gene that produces two male-specific transcripts from two tandem promoters, P1 and P2. Genetic data have identified two sites (loss-of-function (lf) and gain-of-function (gf)) in the P1 promoter that may be regulatory sites for her-1 expression. In order to identify factors involved in the regulation of her-1, two yeast-one-hybrid screens were carried out using the lf  and gf sites. The if screen identified F13G3.1, a putative DNA binding protein containing three TFIIIA-type zinc fingers. The gf screen isolated C26C6.5, the worm homologue of p66, a component of the NuRD complex. NuRD is a nucleosome remodeling and histone deacetylase complex, implying that chromatin remodeling may be involved in the negative regulation of her-1.
Notes: Adviser:  Marc D. Perry.

Thesis (M.Sc.)--University of Toronto, 2004.

Electronic version licensed for access by U. of T. users.

Source: Masters Abstracts International, Volume: 42-06, page: 2105.

MICR copy on microfiche (2 microfiches).