role of CD8+ T cells in the regulation of recipient immune responses induced by allogeneic platelet transfusions.
Published: 2004
Language: eng
Number of pages: 110
Description: The immune mechanisms resulting in platelet-induced alloimmunization are incompletely understood. Using a murine model of platelet transfusion, previous studies suggest allogeneic platelets stimulate early NO-dependent cytotoxicity, while repeated transfusions induce alloantibody production and late cytotoxic responses. To further understand how recipient anti-platelet responses are modulated, the effects of CD8+T cell depletion on cell-mediated cytotoxicity and antibody responsiveness were investigated. Cytotoxicity within BALB/c mice transfused with allogeneic platelets was measured via 6-hour chromium-release assays. Recipient sera was tested for antibody production. Splenic lymphocytes were analyzed, via intracellular staining, for cytokine production. Depletion of CD8+T cells enhanced NO-dependent cytotoxicities. CD8+ depleted recipients exhibited increased alloantibody production, primarily of the IgG2a isotype. Non-depleted recipients produced IgG1 and IgG3. Cytokine analysis revealed the effects of CD8+T cell depletion on recipient responses may be related to cytokine production. These results suggest that CD8+T cells regulate NO-dependent cytotoxic and humoral responses to allogeneic platelet transfusions.
Notes: Adviser:  John W. Semple.

Thesis (M.Sc.)--University of Toronto, 2004.

Electronic version licensed for access by U. of T. users.

Source: Masters Abstracts International, Volume: 42-06, page: 2156.

MICR copy on microfiche.