RNAi
Subtitle: design and application
By: edited by Sailen Barik.
Contributors: Barik, Sailen.
Series: Methods in molecular biology -- v. 442; Methods in molecular biology (Clifton, N.J.) -- v. 442.
Publisher: Humana Press
Published: 2008
Place of publication: Totowa, N.J
Language: eng
Number of pages: 270
ISBN-13: 9781588298744
Subjects: RNA Interference -- Laboratory Manuals; RNA, Small Interfering -- Laboratory Manuals; Gene Silencing -- Laboratory Manuals; Genetic Techniques -- Laboratory Manuals; Small interfering RNA -- Laboratory manuals
Genres: Laboratory Manuals.; Laboratory manuals.
Notes: Includes bibliographical references and index.
Table of contents: title: Principles of dicer substrate (D-siRNA) design and function / Mohammed Amarzguioui and John J. Rossi; title: Expression, purification, and analysis of recombinant Drosophila dicer-1 and dicer-2 enzymes / Xuecheng Ye and Qinghua Liu; title: In vitro RNA cleavage assay for argonaute-family proteins / Keita Miyoshi ... [et al.]; title: Identifying siRNA-induced off-targets by microarray analysis / Emily Anderson ... [et al.]; title: Hydrodynamic delivery of siRNA in a mouse model of sepsis / Doreen E. Wesche-Soldato ... [et al.]; title: Nasal delivery of siRNA / Vira Bitko and Sailen Barik; title: RNA interference as a genetic tool in trypanosomes / Vivian Bellofatto and Jennifer B. Palenchar; title: Lentivirus-mediated RNA interference in mammalian neurons / Scott Q. Harper and Pedro Gonzalez-Alegre; title: Silencing genes by RNA interference in the protozoan parasite Entamoeba histolytica / Carlos F. Solis and Nancy Guillén; title: Use of RNAi in C. elegans / Tsuyoshi Ohkumo ... [et al.]; title: Application of siRNA against SARS in the rhesus macaque model / Qingquan Tang ... [et al.]; title: siRNA and shRNA as anticancer agents in a cervical cancer model / Wenyi Gu, Lisa Putral, and Nigel McMillan; title: Identification and expression analysis of small RNAs during development / Toshiaki Watanabe, Hiroshi Imai, and Naojiro Minami; title: Screening and identification of virus-encoded RNA silencing suppressors / Sumona Karjee, Mohammad Nurul Islam, and Sunil K. Mukherjee; title: Application of RNA interference in functional genomics studies of a social insect / Michael E. Scharf, Xuguo Zhou, and Margaret A. Schwinghammer; title: Temporal control of gene silencing by in ovo electroporation / Thomas Baeriswyl, Olivier Mauti, and Esther T. Stoeckli; title: Altering flower color in transgenic plants by RNAi-mediated engineering of flavonoid biosynthetic pathway / Yoshikazu Tanaka, Noriko Nakamura, and Junichi Togami; title: Transgenic RNA interference in mice / Pumin Zhang.