Increased expression of hypoxia inducible factor-1alpha (HIF-1alpha) and neuronal nitric oxide synthase (nNOS) in the cerebral cortex of anemic rats.
Published: 2006
Language: eng
Number of pages: 92
ISBN-13: 9780494213766
Description: Characterization of the adaptive cellular responses to hemodilutional anemia will increase our understanding of cerebral cortical homeostatic mechanisms. We hypothesize that acute hemodilutional anemia activates adaptive cellular regulatory mechanisms including HIF-1alpha and nNOS within the cerebral cortex. Anesthetized rats underwent isovolemic hemodilution with 10% pentastarch. Plasma TNFalpha, IL-1beta, IL-6 and cerebral cortical hypoxia-inducible factor-1alpha (HIF-1alpha) and neuronal nitric oxide synthase (nNOS) protein levels were characterized.Normotension, normoxemia and normocapnia were all maintained during hemodilution, while the hemoglobin concentration was reduced by 50%. In anemic animals, plasma IL-1beta and cerebral cortical HIF-1alpha and nNOS protein levels were significantly elevated, relative to controls, at 18 hours. Immunofluorescence localized HIF-1alpha to neurons and astrocytes while nNOS localized to neurons. These findings may support a regulatory role for HIF-1alpha and nNOS protein during anemia. Co-localization of these proteins to neurons and astrocytes may suggest that both cell types contribute to maintaining cerebal homeostasis during acute anemia.
Notes: Source: Masters Abstracts International, Volume: 45-03, page: 1397.

Thesis (M.Sc.)--University of Toronto, 2006.

Electronic version licensed for access by U. of T. users.

ROBARTS MICROTEXT copy on microfiche.