Mast cells
Subtitle: methods and protocols
By: edited by Guha Krishnaswamy, David S. Chi
Contributors: Krishnaswamy, Guha; Chi, David S
Series: Methods in molecular biology -- 315; Methods in molecular biology (Clifton, N.J.) -- 315
Publisher: Humana Press
Published: 2006
Place of publication: Totowa, N.J
Language: eng
Number of pages: 439
Subjects: Mast cells -- Laboratory manuals
Genres: Laboratory manuals
Description: A cutting-edge collection of readily reproducible techniques for the isolation, culture, and study of activation and signaling in human mast cells. These methods take advantage of the latest advances in molecular biology, technology, and information science. They include methods for the identification of mast cells, the development of mast cells in vitro, the study of mast cell signaling and gene expression, and the measurement of mast cell expression of inflammatory mediators. Additional chapters cover methods for studying mast cell interactions with other cell types (endothelial cells, fibroblasts, and B cells), the roles of mast cells in host defense, and mast cell apoptosis.
Notes: Includes bibliographical references and index
Table of contents: title: Paul Ehrlich's "Mastzellen"; title: from aniline dyes to DNA chip arrays : a historical review of developments in mast cell research / Harsha Vyas and Guha Krishnaswamy; title: The human mast cell : an overview / Guha Krishnaswamy, Omar Ajitawi, and David S. Chi; title: Mast cells in allergy and autoimmunity : implications for adaptive immunity / Gregory D. Gregory and Melissa A. Brown; title: Analysis of MCT and MCTC mast cells in tissue / Lawrence B. Schwartz; title: Mast cell ultrastructure and staining in tissue / Shruti A. Shukla ... [et al.]; title: Expression of cell surface antigens on mast cells : mast cell phenotyping / Alexander W. Hauswirth ... [et al.]; title: Identification of mast cells in the cellular response to myocardial infarction / Nikolaos G. Frangogiannis and Mark L. Entman; title: Growth of human mast cells from bone marrow and peripheral blood-derived CD34+ pluripotent progenitor cells / Arnold S. Kirshenbaum and Dean D. Metcalfe; title: Culture of human mast cells from hemopoietic progenitors / Hirohisa Saito; title: Isolation, culture, and characterization of intestinal mast cells / Gernot Sellge and Stephan C. Bischoff; title: Activation of nuclear factor kappa B / Chuanfu Li, Jim Kelley, and Tuanzhu Ha; title: Analysis of mitogen-activated protein kinase activation / Stephen C. Armstrong; title: Microarray and gene-clustering analysis / Tsung-Hsien Tsai, Denise M. Milhorn, and Shau-Ku Huang; title: Techniques to study Fc-RI signaling / Yuko Kawakami, Jiro Kitaura, and Toshiaki Kawakami; title: Human mast cell proteases : activity assays using thiobenzyl ester substrates / David A. Johnson; title: Mast cell histamine and cytokine assays / David S. Chi, S. Matthew Fitzgerald, and Guha Krishnaswamy; title: Measurement of mast cell cytokine release by multiplex assay / Kevin F. Breuel and W. Keith De Ponti; title: Assays for histamine-releasing factors : from identification and cloning to discovery of binding partners / Jacqueline M. Langdon and Susan M. MacDonald; title: Assays for nitric oxide expression / William L. Stone, Hongsong Yang, and Min Qui; title: Immunohistological detection of growth factors and cytokines in tissue mast cells / Zhenhong Qu; title: Endothelial cell activation by mast cell mediators / Kottarappat N. Dileepan and Daniel J. Stechschulte; title: Co-culture of mast cells with fibroblasts : a tool to study their cross-talk / Ido Bachelet, Ariel Munitz, and Francesca Levi-Schaffer; title: Detection of class switching and IgE synthesis in human B cells / Jérôme Péné ... [et al.]; title: Gene silencing using small interference RNA in mast cells / Deling Yin and Charles A. Stuart; title: Mast cell activation by lipoproteins / Jim Kelley ... [et al.]; title: Mast cell activation by stress / Ann L. Baldwin; title: In vivo models for studying mast cell-dependent responses to bacterial infection / Christopher P. Shelburne, James B. McLachlan, and Soman N. Abraham; title: Bacterial activation of mast cells / David S. Chi ... [et al.]; title: Activation of mast cells by streptolysin O and lipopolysaccharide / Michael Stassen ... [et al.]; title: Mast cell apoptosis / Alexander Gerbaulet, Karin Hartmann, and Yoseph A. Mekori