Chromatin and chromatin remodeling enzymes.
By: edited by C. David Allis, Carl Wu.
Contributors: Allis, C. David.; Wu, Carl.
Series: Methods in enzymology -- v. 376
Publisher: Elsevier/Academic Press
Published: 2004
Place of publication: San Diego
Number of pages: 458
ISBN-13: 9780121827809
Subjects: Chromatin.; Chromatin -- enzymology.; Chromatin.; Biofísica.; Histone.; Enzymologie.; Chromatine.; Immunochimie.
Table of contents: title: 1. Fluorescence anisotropy assays for analysis of ISWI-DNA and ISWI-nucleosome interactions; title: 2. Biophysical analysis of specific genomic loci assembled as chromatin in vivo; title: 3. Visualization and 3D structure determination of defined sequence chromatin and chromatin remodeling complexes; title: 4. Electron microscopic analysis of the RSC chromatin remodeling complex; title: 5. Use of optical trapping techniques to study single-nucleosome dynamics; title: 6. Single-molecule analysis of chromatin; title: 7. Biochemical and structural characterization of recombinant histone acetyltransferase proteins; title: 8. Use of nuclear magnetic resonance spectroscopy to study structure-function of bromodomains; title: 9. Assays for the determination of structure and dynamics of the interaction of the chromodomain with histone peptides; title: 10. Expression, purification, and biophysical studies of chromodomain proteins; title: 11. Quantitative assays for characterization of the Sir2 family of NAD+-dependent deacetylases; title: 12. Selective HAT inhibitors as mechanistic tools for protein acetylation; title: 13. Histone deacetylase inhibitors: assays to assess effectiveness in vitro and in vivo; title: 14. Immunochemical analysis of chromatin; title: 15. Generation and characterization of antibodies directed against di-modified histones, and comments on antibody and epitope recognition; title: 16. Generation and characterization of methyllysine histone antibodies; title: 17. Tips in analyzing antibodies directed against specific histone tail modifications; title: 18. Histone methylation: recognizing the methyl mark; title: 19. Analysis of genome-wide histone acetylation state and enzyme binding using DNA microarrays; title: 20. Use of chromatin immunoprecipitation assays in genome- wide location analysis of mammalian transcription factors; title: 21. High-throughput screening of chromatin immunoprecipitates using of CpG-island microarrays; title: 22. Chromatin immunoprecipitation in the analysis of large chromatin murine antigen receptor loci; title: 23. The use of chromatin immunoprecipitation assays in genome-wide analyses of histone modifications; title: 24. Sequential chromatin immunoprecipitation from animal tissues; title: 25. Immunofluorescent staining of polytene chromosomes: exploiting genetic tools; title: 26. Indirect immunofluorescent labeling of drosophila polytene chromosomes: visualizing protein interactions with chromatin in vivo; title: 27. X-chromosome inactivation in mouse embryonic stem cells: analysis of histone modifications and transcriptional activity using immunofluorescence and FISH; title: 28. X inactivation in mouse ES cells: histone modifications and FISH.