Manipulating the Mouse Embryo
Subtitle: A Laboratory Manual
Contributors: Andras Nagy (Editor)
Edition: 3 edition
Publisher: Cold Spring Harbor Laboratory Press
Published: December 15, 2002
Language: eng
Format: Hardcover
Number of pages: 764
ISBN-13: 9780879695743
Subjects: Developmental biology; Genetic engineering; Genetics (non-medical); Reference; Laboratory Medicine; Embryos; Science/Mathematics; Science; Mice; Life Sciences - Biology - General; Laboratory manuals; Genetics; Life Sciences - Biology - Developmental Biology; Transgenic mice
First sentence: THESE ARE PROBABLY THE BEST OF TIMES to be studying mouse genetics and embryology.
Description: Provides background information and detailed protocols for developing a mouse colony and using the animals in transgenic and gene-targeting experiments. The protocols list the animals, equipment, and reagents required and step-by-step procedures. Topics include in vitro culture of preimplantation embryos, surgical procedures, the production of chimeras, and the analysis of genome alterations. The third edition adds protocols for cloning mice, modifying embryonic stem cells, intracytoplasmic sperm injection, and cryopreservation of embryos.