Neuraminidase-1, a subunit of the elastin receptor, alters mitogenic growth factor receptors and down-regulates proliferation of arterial smooth muscle cells.
Published: 2006
Language: eng
Number of pages: 111
ISBN-13: 9780494213100
Description: Elastic fibers are extracellular matrix components that contribute to vascular cell quiescence by sequestering certain mitogenic growth factors. The present study demonstrates that neuraminidase-1 (Neu1), a member of the cell surface elastin receptor, can also down-regulate proliferation of human arterial smooth muscle cells (SMCs). Inhibition of endogenous Neu1 induced a significant up-regulation in SMC proliferation. Conversely, treatment of SMCs with an exogenous bacterial neuraminidase (Nase), which mimicked over-expression of Neu1, decreased cellular proliferation. Most notably, the activity of neuraminidase abolished mitogenic responsiveness of human arterial SMCs to PDGF-BB and IGF-II. Furthermore, fibroblasts derived from patients with congenital sialidosis (genetically deficient in Neu1) were significantly more responsive to PDGF-BB and IGF-II than normal fibroblasts. In conclusion, the current findings suggest that Neu1 down-regulates cellular proliferation by: (1) up-regulation of elastic fiber deposition and by (2) de-sialylation of cell-surface receptors for PDGF-BB and IGF-II.
Notes: Source: Masters Abstracts International, Volume: 45-03, page: 1382.

Thesis (M.Sc.)--University of Toronto, 2006.

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