High-frequency ultrasound assessment of cardiac graft rejection in heterotopically transplanted mice.
Published: 2007
Language: eng
Number of pages: 169
ISBN-13: 9780494275009
Description: We explored the utility of ultrasound biomicroscopy (UBM) as an improved approach over the common method of finger palpation for assessing graft rejection in murine heterotopic cardiac grafts in vivo. Syngeneic (C3H->C3H, n=9) and allogeneic (BALB/c->C3H, n=13) grafts were imaged daily using a 30 MHz transducer for 14 days post-transplant or until rejection (median survival time=12 days), respectively. Mean palpation score for allografts significantly differed from isografts on day 7 post-transplant, whereas UBM detected significantly increased left ventricular (LV) wall thicknesses from day 2 onwards and LV dilation from day 10 post-transplant in allografts (P&le;0.001), but not isografts, which is likely due to myocardial edema and immune-mediated rejection. Novel Doppler waveforms of aortic and coronary flow revealed changes associated with LV dilation and diminishing systolic function in rejecting allografts. UBM-derived measurements of LV wall thicknesses provide a non-invasive method for detecting differences between isografts and allografts earlier than finger palpation.
Notes: Source: Masters Abstracts International, Volume: 45-06, page: 3027.

Thesis (M.Sc.)--University of Toronto, 2007.

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