Plant microRNAs
Subtitle: methods and protocols
By: edited by Blake C. Meyers and Pamela J. Green
Series: Springer protocols; Methods in molecular biology -- v. 592; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 592.
Publisher: Humana Press
Published: 2010
Place of publication: New York
Language: eng
Number of pages: 267
ISBN-13: 9781603270045; 9781603270052
Subjects: Plants; Genetics; Methods; Molecular Biology; Plant molecular genetics; Small interfering RNA; Research; Methodology; Small Interfering RNA; Laboratory Manuals; MicroRNAs
Notes: Includes bibliographical references and index.

Also issued online.
Table of contents: title: Piecing the puzzle together : genetic requirements for miRNA biogenesis in Arabidopsis thaliana / Zhixin Xie; title: Prediction of plant miRNA genes / Matthew W. Jones-Rhoades; title: Methods for isolation of total RNA to recover miRNAs and other small RNAs from diverse species / Monica Accerbi ... [et al.]; title: miRNA target prediction in plants / Noah Fahlgren and James C. Carrington; title: A method to discover phased siRNA loci / Michael J. Axtell; title: Directed gene silencing with artificial microRNAs / Rebecca Schwab ... [et al.]; title: Bioinformatics analysis of small RNAs in plants using next generation sequencing technologies / Kan Nobuta ... [et al.]; title: High-throughput approaches for miRNA expression analysis / Cheng Lu and Frédéric Souret; title: In situ detection of miRNAs using LNA probes / Zoltán Havelda; title: Analysis of miRNA modifications / Bin Yu and Xuemei Chen; title: MicroRNA promoter analysis / Molly Megraw and Artemis G. Hatzigeorgiou; title: Computational methods for comparative analysis of plant small RNAs / Gayathri Mahalingam and Blake C. Meyers; title: Biotic stress-associated microRNAs : identification, detection, regulation, and functional analysis / Florence Jay ... [et al.]; title: Abiotic stress-associated miRNAs : detection and functional analysis / Dong-Hoon Jeong ... [et al.]; title: Processing of miRNA precursors / Yukio Kurihara and Yuichiro Watanabe; title: Purification of Arabidopsis argonaute complexes and associated small RNAs / Yijun Qi and Shijun Mi; title: Transient assays for the analysis of miRNA processing and function / Felipe F. de Felippes and Detlef Weigel.