Pattern of HLA allele co-expression influences immunodominance of anti-viral CTL responses.
Published: 2006
Language: eng
Number of pages: 74
ISBN-13: 9780494211977
Description: In order to assess whether co-expression of multiple, as opposed to expression of single, MHC class I alleles influences the pattern of anti-flu CTL epitope recognition and immunodominance (ImDc), doubly HLA transgenic (Tg) mice were established on a H2-K-/-/D-/- background. In flu-infected, doubly Tg A2/B7 or A2/B27 mice, IFN-gamma ELISpot assays with A2/M1.58-66 and B7/NP418-426 or B27/NP383-391 flu epitopes showed strong recognition of both peptides for both alleles. In contrast, in flu-infected doubly Tg B7/B27 mice, a reduced level of B27/NP383-restricted CTL response was detected while there was virtually no change in the response level of B7/NP418-restricted CTL. These results demonstrate a form of ImDe. Subsequent studies suggested that co-expression of HLA-B7 with HLA-B27 may lead to a partial deletion of Vbeta8.1+  B27/NP383-restricted CD8+ T cells in these doubly Tg B7/B27 mice.
Notes: Source: Masters Abstracts International, Volume: 45-03, page: 1448.

Thesis (M.Sc.)--University of Toronto, 2006.

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