The blood-brain and other neural barriers
Subtitle: reviews and protocols
By: edited by Sukriti Nag
Series: Springer protocols; Methods in molecular biology -- 686; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 686.
Publisher: Humana Press
Published: 2011
Place of publication: New York, N.Y
Language: eng
Number of pages: 502
ISBN-13: 9781607619376; 9781607619383
Subjects: Blood-Retinal Barrier; Blood-brain barrier; Blood-Brain Barrier; Laboratory Manuals; Blood-Nerve Barrier
Description: Evolving technologies starting with tracer studies, and more recently with genomics and proleomics, have provided novel information about the molecular properties of cerebral endothelium and astrocytes; however, further studies must be done in animal models of neurological diseases and in humans to get a clearer understanding of the pathogenesis of blood-brain barrier (BBB) breakdown in nervous system diseases. In The Blood-Brain and Other Neural Barriers: Reviews and Protocols, experts in the field present a series of cuttingedge protocols which can be used to study the barriers. Opening with detailed information on components of the neurovascular unit as well as the blood-cerebrospinal, blood-retinal, and blood-nerve barriers, the book continues with meticulous techniques to image the barriers in humans and experimental animals, followed by cutting-edge molecular techniques to study the BBB and novel models to study the barriers, and it concludes with techniques for the delivery of therapeutic agents across the BBB. Written in the highly successful Methods in Molecular Biology series format, protocol chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. --Book Jacket.
Notes: Includes bibliographical references and index.
Table of contents: title: Morphology and properties of brain endothelial cells / Sukriti Nag; title: Morphology and properties of pericytes / Paula Dore-Duffy and Kristen Cleary; title: Morphology and properties of astrocytes / Sukriti Nag; title: The blood-cerebrospinal fluid barrier : structure and functional significance / Conrad E. Johanson, Edward G. Stopa, and Paul N. McMillan; title: The blood-retinal barrier : structure and functional significance / E. Aaron Runkle and David A. Antonetti; title: The blood-nerve barrier : structure and functional significance / Ananda Weerasuriya and Andrew P. Mizisin; title: Detection of multiple proteins in intracerebral vessels by confocal microscopy / Janet L. Manias, Anish Kapadia, and Sukriti Nag; title: Multiparametric magnetic resonance imaging and repeated measurements of blood-brain barrier permeability to contrast agents / Tavarekere N. Nagaraja ... [et al.]; title: Detection of brain pathology by magnetic resonance imaging of iron oxide micro-particles / Daniel C. Anthony ... [et al.]; title: Measuring the integrity of the human blood-brain barrier using magnetic resonance imaging / Andrea Kassner and Rebecca Thornhill; title: Assessing blood-cerebrospinal fluid barrier permeability in the rat embryo / Norman R. Saunders ... [et al.]; title: Detection of blood-nerve barrier permeability by magnetic resonance imaging / Carsten Wessig; title: Isolation of human brain endothelial cells and characterization of lipid raft-associated proteins by mass spectroscopy / Romain Cayrol ... [et al.]; title: Analysis of mouse brain microvascular endothelium using laser capture microdissection coupled with proteomics / Nivetha Murugesan ... [et al.]; title: Molecular and functional characterization of P-glycoprotein in vitro / Gary N.Y. Chan and Reina Bendayan; title: Methods to study glycoproteins at the blood-brain barrier using mass spectrometry / Arsalan S. Haqqani ... [et al.]; title: Novel models for studying the blood-brain and blood-eye barriers in drosophila / Robert L. Pinsonneault ... [et al.]; title: Zebrafish model of the blood-brain barrier : morphological and permeability studies / Brian P. Eliceiri, Ana Maria Gonzalez, and Andrew Baird; title: Methods to assess pericyte-endothelial cell interactions in a coculture model / Gokulan Thanabalasundaram ... [et al.]; title: Isolation and properties of an in vitro human outer blood-retinal barrier model / Robin D. Hamilton and Lopa Leach; title: Isolation and properties of endothelial cells forming the blood-nerve barrier / Yasuteru Sano and Takashi Kanda; title: Treatment of focal brain ischemia with viral vector-mediated gene transfer / Hua Su and Guo-Yuan Yang; title: Blood-brain barrier disruption in the treatment of brain tumors / Marie Blanchette and David Fortin; title: Integrated platform for brain imaging and drug delivery across the blood-brain barrier / Umar Iqbal, Abedelnasser Abulrob, and Danica B. Stanimirovic; title: Targeting the choroid plexus-CSF-brain nexus using peptides identified by phage display / Andrew Baird ... [et al.]