Monoclonal antibodies
Subtitle: methods and protocols
By: edited by Vincent Ossipow, Départment de Biochemie, University of Geneva, Geneva, Switzerland, Nicolas Fischer, Novimmune SA, Plan-les-Ouates, Switzerland
Contributors: Fischer Nicolas, editor; Springer Science+Business Media
Edition: Second Edition.
Series: Methods in Molecular Biology  : -- 1131; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 1131.; Springer protocols (Series)
Publisher: Humana Press
Published: 2014
Place of publication: New York
Language: eng
Number of pages: 575
ISBN-13: 9781627039918; 9781627039925
Copyright date: 2014
Subjects: Monoclonal antibodies; Laboratory manuals; Monoclonal Antibodies; Laboratory Manuals
Description: Monoclonal Antibodies: Methods and Protocols, Second Edition expands upon the previous edition with current, detailed modern approaches to isolate and characterize monoclonal antibodies against carefully selected epitopes. This edition includes new chapters covering the key steps to generate high quality monoclonals via different methods, from antigen generation to epitope mapping and quality control of the purified IgG. Chapters are divided into four parts corresponding to four distinct objectives. Part I covers monoclonal antibody generation, Part II deals with monoclonal antibody expression and purification, Part III presents methods for monoclonal antibody characterization and modification, and Part IV describes selected applications of monoclonal antibodies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Monoclonal Antibodies: Methods and Protocols, Second Edition provides crucial initial steps of monoclonal antibody generation and characterization with state-of-the art protocols.--
Notes: Previous copyright, 2007.

Includes bibliographical references and index.
Table of contents: title: Antigen production for monoclonal antibody generation  -- Giovanni Magistrelli and Pauline Malinge; title: Method for the generation of antibodies specific  for site and posttranslational modifications  -- Hidemasa Goto and Masaki Inagaki; title: Immunization, hybridoma generation, and selection for monoclonal antibody production  -- Hyung-Yong Kim, Alexander Stojadinovic, and Mina J. Izadjoo; title: Hybridoma technology for the generation of rodent mAbs via classical fusion  -- Efthalia Chronopoulou [and three others]; title: Generation of rabbit monoclonal antibodies  -- Pi-Chen Yam and Katherine L. Knight; title: Screening hybridomas for cell surface antigens by high-throughput homogeneous assay and flow cytometry  -- Alejandro Uribe-Benninghoff [and four others]; title: Screening and subcloning of high producer transfectomas using semisolid media and automated colony picker  -- Suba Dharshanan and Cheah Swee Hung; title: Design and generation of synthetic antibody libraries for phage display  -- Gang Chen and Sachdev S. Sidhu; title: Selection and screening using antibody phage display libraries  -- Patrick Koenig and Germaine Fuh; title: Yeast surface display for antibody isolation : library construction, library screening, and affinity maturation  -- James A.Van Deventer and Karl Dane Wittrup; title: Human B cell immortalization for monoclonal antibody production  -- Joyce Hui-Yuen, Siva Koganti, and Sumita Bhaduri-McIntosh; title: Using next-generation sequencing for discovery of high- frequency monoclonal antibodies in the variable gene repertoires from immunized mice  -- Ulrike Haessler and Sai T. Reddy; title: Cloning, reformatting, and small-scale expression of monoclonal antibody isolated from mouse, rat, or hamster hybridoma  -- Jeremy Loyau and François Rousseau; title: Cloning of recombinant monoclonal antibodies from hybridomas in a single mammalian expression plasmid  -- Nicole Müller-Sienerth [and three others]; title: Monoclonal antibody purification by ceramic hydroxyapatite chromatography  -- Larry J. Cummings, Russell G. Frost, and Mark A. Snyder; title: Rapid purification of monoclonal antibodies using magnetic microspheres  -- Pauline Malinge and Giovanni Magistrelli; title: Generation of cell lines for monoclonal antibody production  -- Krista Alvin and Jianxin Ye; title: Expression and purification of recombinant antibody formats and antibody fusion proteins  -- Martin Siegemund [and four others]; title: Purification of antibodies and antibody fragments using CaptureSelect [trademark] affinity resins  -- Pim Hermans, Hendrik Adams, and Frank Detmers; title: Reformatting of scFv antibodies into the scFv-Fc  format and their downstream purification  -- Emil Bujak [and three others]; title: Antibody V and C domain sequence, structure, and interaction analysis with special reference to IMGT®  -- Eltaf Alamyar [and three others]; title: Measuring antibody affinities as well as the active concentration  of antigens present on a cell surface   -- Palaniswami Rathanaswami; title: Determination of antibody structures  -- Robyn L. Stanfield; title: Affinity maturation of monoclonal antibodies by multi-site- directed mutagenesis  -- Hyung-Yong Kim, Alexander Stojadinovic, and Mina J. Izadjoo; title: Epitope mapping with membrane-bound synthetic overlapping peptides  -- Terumi Midoro-Horiuti and Randall M. Goldblum; title: Epitope mapping by epitope excision, hydrogen/deuterium exchange, and peptide-panning techniques combined with in silico analysis -- Nicola Clementi [and five others]; title: Fine epitope mapping based on phage display and extensive mutagenesis of the target antigen  -- Gertrudis Rojas; title: Epitope mapping with random phage display library   -- Terumi Midoro-Horiuti and Randall M. Goldblum; title: Epitope mapping of monoclonal and polyclonal antibodies using bacterial cell surface display  -- Anna-Luisa Volk, Francis Jingxin Hu, and Johan Rockberg; title: Ion exchange-high-performance liquid chromatography (IEX-HPLC)  -- Marie Corbier, Delphine Schrag, and Sylvain Raimondi; title: Size exclusion-high-performance liquid chromatography (SEC-HPLC)  -- Delphine Schrag, Marie Corbier, and Sylvain Raimondi; title: N-glycosylation characterization by liquid chromatography with mass spectrometry  -- Song Klapoetke; title: Fc engineering of antibodies and antibody derivatives by primary sequence alteration and their functional characterization  -- Stefanie Derer [and six others]; title: Labeling and use of monoclonal antibodies in immunofluorescence : protocols for cytoskeletal and nuclear antigens  -- Christoph R. Bauer; title: Generation and use of antibody fragments for structural studies of proteins refractory to crystallization   -- Stephen J. Stahl, Norman R. Watts, and Paul T. Wingfield; title: Antibody array generation and use -- Carl A.K. Borrebaeck and Christer Wingren.