Interactions between PP1c[gamma]2 and putative testis specific regulatory subunits.
Published: 2004
Language: eng
Number of pages: 110
Description: The gamma2 isoform of the type-1 Protein Phosphatase is a major serine/threonine phosphatase in the murine testis. Male mice lacking this phosphatase suffer from infertility as a result of defects in spermatogenesis. Members of this family of phosphatases share a high degree of sequence identity and possess unique structural arrangements of a single catalytic domain, such as the PP1cgamma protein, in conjunction with one more regulatory subunits. For this thesis, I performed a deletion series analysis of the unique carboxy-terminal domain of PP1cgamma2 to characterize the important regions needed for its interactions with four previously identified putative regulatory subunits. I identified a five amino acid sequence that is required for all of the interactions. Further, I show that the putative regulatory subunits are able to re-localize PP1cgamma2 in vivo from its natural non-specific distribution to either the nucleus or cytoplasm.
Notes: Adviser:  Sue Varmuza.

Thesis (M.Sc.)--University of Toronto, 2004.

Electronic version licensed for access by U. of T. users.

Source: Masters Abstracts International, Volume: 42-06, page: 2204.

MICR copy on microfiche (2 microfiches).