Using chemical genetics to probe Caenorhabditis elegans  biology.
Published: 2006
Language: eng
Number of pages: 87
ISBN-13: 9780494211564
Description: A screen of 10,000 chemicals on C. elegans strain CB4856 was conducted to assess if chemical genetics can be conducted in C. elegans. Out of this screen, 22 chemicals that induced an array of phenotypes were identified. One of the chemicals, named Nemadipine A, induced dramatic morphological and egg-laying defects and was later shown to specifically target egl-19, which encodes an L-type calcium channel in  C. elegans. Another chemical 185G3 is a strong candidate to probe embryogenesis since it induces a phenotype that phenocopies mutation described as embryonic lethality. In addition, the sensitivity assays conducted display evidence of pharmacogenetic variation in C. elegans. This chemical screen demonstrates that chemical genetics can be done in  C. elegans and strengthens this organism's value as a useful platform for drug and target discovery.
Notes: Source: Masters Abstracts International, Volume: 45-03, page: 1370.

Advisor: Peter McCourt.

Botany thesis.

Thesis (M.Sc.)--University of Toronto, 2006.

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