Physical techniques in biological research
Subtitle: Physical chemical techniques
By: edited by Dan H. Moore ; contributors, J. Manuel Denuce [and seven others]
Edition: Second edition.
Publisher: Academic Press
Published: 1968
Place of publication: New York; London
Language: eng
Number of pages: 404
ISBN-13: 9781483167411; 9781483194899
Subjects: Biology; Technique; Experimental Biology; SCIENCE; Life Sciences
Description: Physical Techniques in Biological Research, Volume II, Part A: Physical Chemical Techniques focuses on physical chemical techniques that have been most widely used in the study of molecules of biological significance. This book outlines the theoretical basis of the methods, describes the apparatus and manipulations used, and describes the applications of the techniques by examples. Organized into seven chapters, this volume begins with an overview of the basic property that makes the use of isotopes as tracers possible. This text then explains the predicted behavior during separations of chemi.
Notes: Includes bibliographical references at the end of each chapters and indexes.
Table of contents: title: Front Cover; Physical Chemical Techniques; Copyright Page; List of Contributors; PREFACE TO FIRST EDITION OF VOLUME II; PREFACE TO THE SECOND EDITION OF VOLUME II; Table of Contents; CHAPTER 1. Tracer Techniques: Stable and Radioactive Isotopes; I. Introduction; II. Elements of Radioactivity; III. The Measurement of Radioactivity; IV. Radioàutography; V. Safe Handling of Radioactive Isotopes; VI. The Measurement of the Heavy Stable Isotopes; VII. The Useful Tracer Isotopes and Their Properties; VIII. The Synthesis of Labeled Compounds; IX. The Scope of the Tracer Technique.; title: X. Some Limitations of the Tracer TechniqueREFERENCES; CHAPTER 2. Ultracentrifugation; I. Introduction; II. Molecular Weight Determinations; III. Sedimentation Velocity; IV. Density Gradient Sedimentation; V. Hydrostatic Pressure Effects; REFERENCES; CHAPTER 3. Diffusion in Liquids; I. Diffusion-a Stochastic Process; II. Experimental Methods; III. Diffusiometry with Astigmatic Rayleigh Interference Optics; IV. Applications; REFERENCES; CHAPTER 4. Electrophoresis; I. Introduction; II. Microscopic Method; III. Early Moving Boundary Apparatus; IV. The Tiselius Apparatus; V. Optical Systems.; title: VI. Electrophoretic ProcedureVII. The Measurement of Conductivity and Calculation of Mobility; VIII. Some Hints on Interpretation of Patterns; IX. Applications of Moving Boundary Electrophoresis; X. Separatory Electrophoresis Techniques; REFERENCES; CHAPTER 5. Electrophoresis of Proteins and Peptides in Stabilizing Media; I. Introduction; II. Theoretical Considerations on Electrophoresis in Stabilizing Media; III. Description of the Supporting Media; IV. Special Methods; REFERENCES; CHAPTER 6. Surface Film Techniques; I. Films at the Air-Water Interface; II. Interfacial Films.; title: III. Transfer of Surface Films from a Liquid onto a Solid SurfaceIV. Thickness of Surface Films; V. Surface Potential; VI. Chemical Reactivity of Surface Films; VII. Retardation of Evaporation by Monolayers; VIII. Absorption Spectra of Surface Films; IX. Irradiation of Surface Films of Proteins; ACKNOWLEDGMENTS; REFERENCES; CHAPTER 7. Electrical Potential Differences; I. Introduction; II. Basic Definitions and Units; III. Theory of Reversible Cells; IV. Junction Potentials; V. ""Donnan"" Equilibria and Potentials; VI. Membrane Potentials; VII. Phase Boundary Potentials between Liquids.; title: VIII. Summary of Formulae for Electrical PotentialsIX. The Meaning and Eleetrometrie Measurement of pH; X. The Measurement of Potential Differences; XI. Preparation and Use of Electrodes; NOMENCLATURE; REFERENCES; APPENDIX; Author Index; Subject Index.