The involvement of EGF, EGFr, MSX1, PAX9 and LEF1 in EL mouse third molar hypodontia.
Published: 2004
Language: eng
Number of pages: 89
Description: The EL mouse is an excellent model to study hypodontia because 97% of these animals lack third molars (3M). EGF, Msx1, Pax9 and Lef1 have roles in tooth formation. One purpose of this study was to map EGF/EGFr expression during 3M development, in order to determine the link between 3M hypodontia and EGF/EGFr. The other was to associate Lef1, Msx1 and Pax9 mutations and 3M hypodontia. EGF/EGFr expression was examined using in situ hybridization in normal and EL mice. Different patterns of EGF and EGFr were seen in the mesenchymal and epithelial tissues during all developmental stages with weak expression after P9. The most striking difference was at the 3M EL P7 (bell) stage, when EGF was relatively absent although receptor was present. After comparing EL and normal mouse cDNA sequences for Msx1, Pax9 and Lef1, a threonine170serine substitution mutation is predicted to have occurred in Lef1. This study suggests possible roles for EGF and Lef1 in EL mice 3M hypodontia.
Notes: Adviser: G. V. Kulkarni.

Thesis (M.Sc.)--University of Toronto, 2004.

Electronic version licensed for access by U. of T. users.

Source: Masters Abstracts International, Volume: 43-03, page: 0811.

MICR copy on microfiche (1 microfiche).