Single-molecule and super-resolution fluorescence studies of the structure and function of telomerase and telomere
Contributors: Zhuang, Xiaowei; Murray, Andrew W.; Francis, Nicole J.; Cohen, Adam E.
Published: 2012
Language: eng
Description: Telomerase and telomere play crucial roles in the maintenance of genomic stability. Through its ability to extend chromosome ends with G-rich telomeric sequence, telomerase solves the end-replication problem of linear chromosomes and allows complete replication of the genetic information. Telomere along with its protein partners solves the end-protection problem and guards the chromosome ends against aberrant DNA damage response. In this thesis, I present two single-molecule fluorescence-based studies that determined the functional structure of telomerase RNA within active telomerase holoenzyme and probed the structure of telomere and its dependence on telomere binding proteins.
Notes: Keywords: FRET, single-molecule, STORM, telomerase, telomere, Xist.

Thesis Ph.D. Harvard University 2012