DNA repair protocols
Subtitle: mammalian systems
By: edited by Daryl S. Henderson
Contributors: Henderson, Daryl S
Edition: 2nd ed
Series: Methods in molecular biology -- 314; Methods in molecular biology (Clifton, N.J.) -- 314
Publisher: Humana Press
Published: 2006
Place of publication: Totowa, N.J
Language: eng
Number of pages: 498
Subjects: DNA repair -- Laboratory manuals
Genres: Laboratory manuals
Description: This collection of readily reproducible techniques for repairing mammalian DNA contains fourteen entirely new chapters and many extensively revised chapters. The methods presented cover cytogenetic analysis, measuring the cellular response to ionizing radiation, detecting single-strand (nicks) and double-strand DNA breaks, detecting the presence of adducted bases in DNA, and preparing mismatch repair (MMR) plasmid substrates. Among the highlights are excellent coverage of both base excision repair (BER) and nucleotide excision repair (NER), useful assays for identifying and quantifying UV-induced DNA lesions and DNA breakage, gene therapy, environmental mutagenesis and cancer, and gene targeting.
Notes: Includes bibliographical references and index
Table of contents: title: Isolation of mutagen-sensitive Chinese hamster cell lines by replica plating / Małgorzata Z. Zdzienicka; title: Complementation assays adapted for DNA repair/deficient keratinocytes / Mathilde Fréchet ... [et al.]; title: Cytogenetic challenge assays for assessment of DNA repair capacities / William Au and Salama A. Salama; title: Evaluating the delayed effects of cellular exposure to ionizing radiation / Shruti Nagar, James J. Corcoran, and William F. Morgan; title: Inhibition of DNA synthesis by ionizing radiation: a marker for an S-phase checkpoint / Nicolaas G.J. Jaspers and Małgorzata Z. Zdzienicka; title: Analysis of inhibition of DNA replication in irradiated cells using the SV40 based in vitro assay of DNA replication / George Iliakis, Ya Wang, and Hong Yan Wang; title: Cytometric assessment of histone H2AX phosphorylation, a reporter of DNA damage / Xuan Huang and Zbigniew Darzynkiewicz; title: Detection of DNA strand breaks by flow and laser scanning cytometry in studies of apoptosis and cell proliferation (DNA replication) / Zbigniew Darzynkiewicz, Xuan Huang, and Masaki Okafugi; title: In vitro rejoining of double-strand breaks in genomic DNA / George Iliakis and Nge Cheong; title: Detection of DNA double-strand breaks and chromosomal translocations using ligation-mediated and inverse PCR / Michael J. Villalobos, Christopher J. Betti, and Andrew T.M. Vaughan; title: Plasmid-based assays for DNA end joining in vitro / George Iliakis ... [et al.]; title: Use of gene targeting to study recombination in mammalian DNA repair mutants / Rodney Nairn and Gerald Adair; title: Gene-specific and mitochondrial repair of oxidative DNA damage / R. Michael Anson, Penelope A. Mason, and Vilhelm A. Bohr; title: Quantitative PCR-based measurement of nuclear and mitochondrial DNA damage and repair in mammalian cells / Janine H. Santos ... [et al.]; title: Measuring the formation and repair of DNA damage by ligation-mediated PCR / Gerd P. Pfeifer; title: Immunochemical detection of UV-induced DNA damage and repair / Marcus S. Cooke and Alistair Robson; title: A dot-blot immunoassay for measuring repair of UV photoproducts / Shirley McCready; title: Quantification of photoproducts in mammalian cell DNA using radioimmunoassay / David L. Mitchell; title: DNA damage quantitation by alkaline gel electrophoresis / Betsy M. Sutherland, Paula V. Bennett, and John C. Sutherland; title: The comet assay: a sensitive genotoxicity test for the detection of DNA damage and repair / Günter Speit and Andreas Hartmann; title: Fast micromethod DNA single-strand break assay / Heinz C. Schröder ... [et al.]; title: 32P-postlabeling DNA-damage assays: PAGE, TLC, and HPLC / Shinya Shibutani, Sung Yeon Kim, and Naomi Suzuki; title: Electrophoretic mobility shift assays to study protein binding to damaged DNA / Vaughn Smider, Byung Joon Hwang, and Gilbert Chu; title: Construction of MMR plasmid substrates and analysis of MMR error correction and excision / Huixian Wang and John B. Hays; title: Analysis of enzymes that act at abasic sites in DNA / Walter A. Deutsch and Vijay Hegde; title: Base excision repair in mammalian cells / Yoshihiro Matsumoto; title: In vitro base excision repair assay using mammalian cell extracts / Guido Frosina ... [et al.]; title: Biochemical assays for the characterization of DNA helicases / Robert M. Brosh Jr. and Sudha Sharma; title: Repair synthesis assay for nucleotide excision repair activity using fractionated cell extracts and UV-damaged plasmid DNA / Maureen Biggerstaff and Richard D. Wood; title: Assaying for the dual incisions of nucleotide excision repair using DNA with a lesion at a specific site / Mahmud K.K. Shivji ... [et al.]; title: Analysis of proliferating cell nuclear antigen (PCNA) associated with DNA excision repair sites in mammalian cells / A. Ivana Scovassi and Ennio Prosperi; title: Analysis of DNA repair and chromatin assembly in vitro using immobilized damaged DNA substrates / Jill A. Mello, Jonathan G. Moggs, and Geneviève Almouzni