Cell cycle control
Subtitle: mechanisms and protocols
By: edited by Tim Humphrey, Gavin Brooks
Contributors: Humphrey, Tim; Brooks, Gavin
Series: Methods in molecular biology -- 296; Methods in molecular biology (Clifton, N.J.) --  296
Publisher: Humana Press
Published: 2005
Place of publication: Totowa, N.J
Language: eng
Number of pages: 402
Subjects: Cell cycle -- Laboratory manuals
Genres: Laboratory manuals
Description: Details emerging methodologies for studying the cell cycle, its kinases, and kinase inhibitors. The authors focus the issues of gene expression in vivo and in vitro, the analysis of cyclin-dependent kinase inhibitors, protein degradation mediated by the proteosome, the analysis of the transformed cell phenotype, and innovative techniques to detect apoptosis. Many of the protocols are new, such as electron microsocopy to detect apoptosis and proteosome-mediated degradation, whereas others study the interactions between protein-protein, protein-DNA, and protein-RNA.
Notes: Includes bibliographical references and index
Table of contents: title: Cell cycle molecules and mechanisms of the budding and fission yeasts / Tim Humphrey and Amanda Pearce; title: The plant cell cycle : an overview / John H. Doonan; title: The C. elegans cell cycle / Sander van den Heuvel; title: Developmental control of growth and cell cycle progression in drosophila / Lisa Swanhart, Jeremy Kupsco, and Robert J. Duronio; title: The xenopus cell cycle : an overview / Anna Philpott and P. Renee Yew; title: The mammalian cell cycle : an overview / Jane V. Harper and Gavin Brooks; title: Synchronization of cell populations in G1/S and G2/M phases of the cell cycle / Jane V. Harper; title: Mapping origins of DNA replication in eukaryotes / Susan A. Gerbi; title: In situ assay for analyzing the chromatin binding of proteins in fission yeast / Stephen E. Kearsey ... [et al.]; title: Application of magnetic beads to purify cells transiently transfected with plasmids encoding short hairpin RNAs / Peter D. Adams; title: The cell cycle and virus infection / Steven R. Emmett ... [et al.]; title: Methods for preparation of proteins and protein complexes that regulate the eukaryotic cell cycle for structural studies / Julie Welburn and Jane Endicott; title: E2F transcription factors and pRb pocket proteins in cell cycle progression / Ludger Hauck and Rüdiger von Harsdorf; title: Forkhead (FOX) transcription factors and the cell cycle : measurement of DNA binding by FoxO and FoxM transcription factors / Katrina A. Bicknell; title: Measurement of geminin activity in xenopus egg extracts / Thomas J. McGarry; title: CDK-activating kinases : detection and activity measurements / Stéphane Larochelle and Robert P. Fisher; title: Cyclins, cyclin-dependent kinases, and cyclin-dependent kinase inhibitors : detection methods and activity measurements / Gavin Brooks; title: Measurement of wee kinase activity / Paul R. Mueller and Walter F. Leise III; title: CDC25 dual-specificity protein phosphatases : detection and activity measurements / Sorab N. Dalal and Melanie Volkening; title: Assaying cell cycle checkpoints : activity of the protein kinase Chk1 / Carmela Palermo and Nancy C. Walworth; title: Polo-like kinase-1 : activity measurement and RNAi-mediated knockdown / Marcel A.T.M. van Vugt and René H. Medema; title: The Ipl1/aurora kinase family : methods of inhibition and functional analysis in mammalian cells / Claire Ditchfield, Nicholas Keen, and Stephen S. Taylor; title: Purification of the Ndc80 kinetochore subcomplex from xenopus eggs / Mark L. McCleland and P. Todd Stukenberg