Regulation of alternative splicing during the cell cycle.
Published: 2004
Language: eng
Number of pages: 100
Description: Alternative splicing generates mRNA isoforms from a single precursor mRNA, and is thought to be a key step in generating proteomic diversity. Recent evidence indicates that transcriptional activators function in the regulation of splicing, however to what extent this occurs within the cell is unknown. Reported here are studies investigating the role of E2F1 in the constitutive and alternative splicing of endogenous target genes. Furthermore, global effects of E2F1 expression on alternative splicing, and the global regulation of alternative splicing during the human cell cycle were investigated using a novel microarray platform. The results of these studies provide evidence that E2F1 impacts the splicing of its target genes, and for the existence of cell cycle regulated alternative splicing events. These results set the stage for future studies investigating the mechanisms by which activators influence the splicing of target genes, and the possible roles of alternative splicing in cell cycle progression.
Notes: Adviser:  Benjamin Blencowe.

Thesis (M.Sc.)--University of Toronto, 2004.

Electronic version licensed for access by U. of T. users.

Source: Masters Abstracts International, Volume: 43-03, page: 0787.

MICR copy on microfiche (2 microfiches).