Maternal RNA stability analyzed using gene expression profiling in Drosophila.
Published: 2004
Language: eng
Number of pages: 198
Description: The early development of the Drosophila embryo is controlled by maternal mRNA. At the midblastula transition there are two classes of maternal mRNA, a stable and an unstable class. In this thesis a time course of microarray experiments using the time period from Stage 14 oocytes to 4--6hr after an egg has been deposited, was generated for both wild-type and smg  mutant unfertilized eggs. It was determined that approximately 31% of the Drosophila genome is maternally expressed. Approximately 16% of these maternal transcripts were found to be unstable in wild-type unfertilized eggs. Previously it has been determined that in the smg mutant, the unstable transcripts, Hsp83 and nanos are stabilized. In the smg mutant there are about a quarter fewer unstable transcripts, indicating that SMG may be implicated in the degradation of many maternal transcripts.
Notes: Adviser:  H. Lipshitz.

Thesis (M.Sc.)--University of Toronto, 2004.

Electronic version licensed for access by U. of T. users.

Source: Masters Abstracts International, Volume: 43-03, page: 0786.

MICR copy on microfiche (3 microfiches).