Membrane trafficking
By: edited by Ales Vancura.
Contributors: Vancura, Ales.
Series: Methods in molecular biology -- 457; Methods in molecular biology (Clifton, N.J.) -- v. 457.
Publisher: Humana; Springer [distributor]
Published: 2008
Place of publication: Totowa, N.J; London
Language: eng
Number of pages: 405
ISBN-13: 9781588299253
Subjects: Biological transport; Biological Transport -- genetics -- Laboratory Manuals; Biological Transport -- physiology -- Laboratory Manuals; Cell Membrane -- genetics -- Laboratory Manuals; Cell Membrane -- physiology -- Laboratory Manuals; Protein Transport -- genetics -- Laboratory Manuals; Protein Transport -- physiology -- Laboratory Manuals; Membrane Transport Proteins -- analysis -- Laboratory Manuals; Cytological Techniques -- Laboratory Manuals
Genres: Laboratory Manuals.
Notes: Includes bibliographical references and index.
Table of contents: title: Pt. I. Yeast Cells; title: Ch. 1. Freight management in the cell / Johannes M. Herrmann, Anne Spang; title: Ch. 2. High-throughput protein extraction and immunoblotting analysis in saccharomyces cerevisiae / Todd C. Lorenz, Vikram C. Anand, Gregory S. Payne; title: Ch. 3. Genome-wide analysis of membrane transport using yeast knockout arrays / Helen E. Burston, Michael Davey, Elizabeth Conibear; title: Ch. 4. A cell-free system for reconstitution of transport between prevacuolar compartments and vacuoles in saccharomyces cerevisiae / Thomas A. Vida; title: Ch. 5. In vitro analysis of the mitochondrial preprotein import machinery using recombinant precursor polypeptides / Dorothea Becker, Martin Krayl, Wolfgang Voos; title: Ch. 6. In vitro import of proteins into isolated mitochondria / Karl Bihlmaier, Melanie Bien, Johannes M. Herrmann; title: Ch. 7. Synthesis and sorting of mitochondrial translation products / Heike Bauerschmitt, Soledad Funes, Johannes M. Herrmann; title: Ch. 8. In vivo labeling and analysis of mitochondrial translation products in budding and in fission yeasts / Karine Gouget, Fulvia Verde, Antoni Barrientos; title: Ch. 9. Exploring protein-protein interactions involving newly synthesized mitochondrial DNA encoded proteins / Darryl Horn, Flavia Fontanesi, Antoni Barrientos; title: Ch. 10. Purification of yeast membranes and organelles by sucrose density gradient centrifugation / Jennifer Chang, Victoria Ruiz, Ales Vancura; title: Ch. 11. Microscopic analysis of lipid droplet metabolism and dynamics in yeast / Heimo Wolinski, Sepp D. Kohlwein; title: Ch. 12. Use of bimolecular fluorescence complementation (BiFC) in yeast saccharomyces cerevisiae / Kari-Pekka Skarp, ... [et al.]; title: Pt. II. Mammalian cells; title: Ch. 13. Using quantitative fluorescence microscopy to probe organelle assembly and membrane trafficking / Brian Storrie, Tregei Starr, Kimberly Forsten-Williams; title: Ch. 14. Measuring secretory membrane traffic / Vytaute Starkuviene, ... [et al.]; title: Ch. 15. A correlative light and electron microscopy method based on laser micropatterning and etching / Julien Colombelli, ... [et al.]; title: Ch. 16. Approaches to investigate the role of signaling in ER-to-Golgi transport / Laura J. Sharpe, Ximing Du, Andrew J. Brown; title: Ch. 17. Recruitment of coat proteins to peptidoliposomes / Gregor Suri, Martin Spiess, Pascal Crottet; title: Ch. 18. SNAREs mediated fusion of liposomes / Jérôme Vicogne, Jeffrey E. Pessin; title: Ch. 19. Use of polarized PC12 cells to monitor protein localization in the early biosynthetic pathway / Ragna Sannerud, ... [et al.]; title: Ch. 20. Tracking the transport of E-cadherin to and from the plasma membrane / Matthew P. Wagoner, Kun Ling, Richard A. Anderson; title: Ch. 21. Analysis of nucleocytoplasmic shuttling of NFkB proteins in human leukocytes / Chandra C. Ghosh ... [et al.]; title: Ch. 22. Imaging pHluorin-based probes at hippocampal synapses / Stephen J. Royle, ... [et al.]; title: Ch. 23. Analysis of receptor tyrosine kinase internalization using flow cytometry / Ning Li, Kristen S. Hill, Lisa A. Elferink; title: Ch. 24. Measurement of receptor endocytosis and recycling / Jane M. Knisely, Jiyeon Lee, Guojun Bu; title: Ch. 25. Styryl dye-based synaptic vesicle recycling assay in cultured cerebellar granule neurons / Victor Anggono, Michael A. Cousin, Phillip J. Robinson; title: Ch. 26. Use of quantitative immunofluorescence microscopy to study intracellular trafficking / Vincent Blot, Timothy E. McGraw; title: Ch. 27. Dissecting GLUT4 traffic components in L6 myocytes by fluorescence-based, single cell assays / Costin N. Antonescu, Varinder K Randhawa, Amira Klip; title: Ch. 28. Functional genetic analysis of the mammalian mitochondrial DNA encoded peptides / Maria Pilar Bayona-Bafaluy, ... [et al.].