Human cell culture protocols
By: edited by Ragai R. Mitry, Robin D. Hughes
Edition: 3rd ed.
Series: Methods in molecular biology -- 806; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 806.; Springer protocols
Publisher: Humana  Press
Published: 2012
Place of publication: New York
Language: eng
Number of pages: 435
ISBN-13: 9781617793660; 9781617793677
Subjects: Human cell culture; Laboratory manuals
Notes: Includes bibliographical references and index.
Table of contents: title: 1. Introduction to cell culture / Christina Philippeos, Robin D. Hughes, Anil Dhawan and Ragai R. Mitry; title: 2. Isolation and cultivation of dermal stem cells that differentiate into functional epidermal melanocytes / Ling Li, Mizubo Fukunaaga; title: 3. Isolation, cultivation, and application of human alveolar epithelial cells / Nicole Daum, Anna Kuehn, Stephanie Hein, Ulrich F. Schaefer, Hanno Huwer, and Claus-Michael Lehr; title: 4. Culture of parathyroid cells / Peyman Björklund and Per Hellman; title: 5. Functional analysis of human islets of langerhans maintained in culture / Shanta J. Persaud, Bo Liu, and Peter M. Jones; title: 6. Conversion of non-endocrine human pancreatic cells to insulin-producing cells for treatment of diabetes / Min Zhao and Guo Cai Huang; title: 7. Evaluation of cytochrome P450 activities in human hepatocytes in vitro / María José Gómez-Lechón, Agustín Lahoz, José V. Castell, and María Teresa Donato; title: 8. Human hepatocytes: isolation, culture, and quality procedures / Daniel Knobeloch, Sabrina Ehnert, Lilianna Schyschka, Peter Büchler, Michael Schoenberg, Jörg Kleeff, Wolfgang E. Thasler, Natascha C. Nussler, Patricio Godoy, Jan Hengstler, and Andrea K. Nussler; title: 9. Preclinical testing ov virotherapeutics for primary and secondary tumors of the liver / Martina Zimmermann, Timo Weiland, Michael Bitzer, and Ulrich M. Lauer; title: 10. Isolation and functional studies of human fetal gastric epithelium in primary culture / Pierre Chailler, Jean-Franc̦ois Beaulieu, and Daniel Ménard; title: 11. Isolation, characterization, and culture of normal human intestinal crypt and villus cells / Jean-Franc̦ois Beaulieu and Daniel Ḿenard; title: 12. Primary culture of human renal proximal tubule epithelial cells and interstitial fibroblasts / Claire C. Sharpe and Mark E.C. Dockrell; title: 13. Glomerular epithelial and mesangial cell culture and characterization / Heather M. Wilson and Keith N. Stewart; title: 14. Culture of isolated human adipocytes and isolated adipose tissue / Kirstin A. Carwswell, Mi-Jeong Lee, and Susan K. Fried; title: 15. Primary culture of human adipocyte precursor cells: expansion  and differentiation / Thomas Skurk and Hans Hauner; title: 16. Primary culture of ovarian cells for research on cell interactions in the hormonal control of steroidogenesis / Jerzy F. Galas; title: 17. Human vascular smooth muscle cell culture / Diane Proudfoot and Catherine Shanahan; title: 18. Culture of human endothelial cells from umbilical veins / Richard C.M. Siow; title: 19. Human peripheral blood mononuclear cell culture for flow cytometric analysis of phosphorylated mitogen-activated protein kinases / Athanasios Mavropoulos, Daniel Smyk, Eirini I. Rigopoulou, and Dimitrios P. Bogdanos; title: 20. Human CD4+CD25(high [superscript]) CD127 (low/neg [superscript]) regulatory T cells / Haibin Su, Maria Serena Longhi, Pengyun Wang, Diego Vergani, and Yun Ma; title: 21. Human chondrocyte cultures as models of cartilage-specific gene regulation / Miguel Otero, Marta Favero, Cecilia Dragomir, Karim El Hachem, Ko Hashimoto, Darren A. Plumb, and Mary B. Goldring; title: 22. Isolation and culture of human osteoblasts / Alison Gartland, Robin M.H. Rumney, Jane P. Dillon, and James A. Gallagher; title: 23. Human osteoclast culture and phenotypic characterization / Ankita Agrawal, James A. Gallagher, and Alison Gartland; title: 24. Effects of temperature generated from the holmium: Yag laser on human osteoblasts in monolayer tissue culture / Moustafa I. Hafez, Anne Sandison, Richard R. H. Coombs, Ian D. McCarthy, and Al-Shymaa M. Hafez; title: 25. Laser microdissection microscopy: application to cell culture / Ahlam Mustafa, Cathy Cenayko, Ragai R. Mitry, and Alberto Quaglia; title: 26. Automated adherent human cell culture (mesenchymal stem cells) / Robert Thomas and Elizabeth Ratcliffe; title: 27. Culturing and differentiating human mesenchymal stem cells for biocompatible scaffolds in regenerative medicine / William R. Otto and Catherine E. Sarraf.