Type 2 diabetes
Subtitle: methods and protocols
By: edited by Claire Stocker
Series: Methods in molecular biology -- 560; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 560.; Springer protocols
Publisher: Humana Press
Published: 2009
Place of publication: New York; London
Language: eng
Number of pages: 278
ISBN-13: 9781934115152; 9781597454483
Notes: Includes bibliographical references and index.
Table of contents: title: 1. Selecting the "right" mouse model for metabolic syndrome and type 2 diabetes research / Edward H. Leiter; title: 2. Nutritional models of type 2 diabetes mellitus / Beverly Sara Muhlhausler; title: 3. The isolation and purification of rodent pancreatic islets of Langerhans / Jacqueline F. O ̓Dowd; title: 4. The measurement of insulin secretion from isolated rodent islets of Langerhans / Anna L. Nolan and Jacqueline F. O ̓Dowd; title: 5. The incubation and monitoring of cell viability in primary rat islets of Langerhans and pancreatic B-cell lines / Noel G. Morgan, Eleftheria Diakogiannaki, and Mark A. Russell; title: 6. In vitro culture of isolated islets of Langerhans : analysis of function  / Anna L. Nolan; title: 7. Single cell RT-PRC identification of genes expressed by human islet endocrine cells / Dany Muller, Peter M. Jones, and Shanta J. Persaud; title: 8. Laser capture microdissection of human pancreatic B-cells and RNA preparation for gene expression profiling / Lorella Marselli ... [et al.]; title: 9. In vitro transdifferentiation of human hepatoma cells into pancreatic-like cells / Wan-chun Li; title: 10. The measurement of GLUT4 translocation in 3T3-L1 adipocytes / Nicky Konstantopoulos and Juan Carlos Molero-Navajas; title: 11. Pancreatic remodeling : beta cell apoptosis, proliferation and neogenesis and the measurement of beta cell mass and of individual beta cell size / Eduard Montanya and Noélia Téllez; title: 12. Morphology of pancreatic islets : a time course of pre-diabetes in Zucker fatty rats / Petra Augstein and Eckhard Salzsieder; title: 13. Fluorescent Immunohistochemistry and in situ hybridization analysis of pancreas / Xiuli Wang, Shundi Ge, and Gay M. Crooks; title: 14. The measurement of insulin secretion using pancreas perfusion in the rodent / Edward T. Wargent; title: 15. Hyperinsulinemic-euglycemic clamp to assess insulin  sensitivity in vivo / Jason K. Kim; title: 16. Gene expression analysis in diabetes research / Peter White and Klaus H. Kaestner; title: 17. Gene expression mining in type 2 diabetes research / Donald R. Dunbar.