DNA repair protocols
By: edited by Lotte Bjergbæk
Edition: 3rd ed.
Series: Methods in molecular biology -- 920; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- 920.; Springer protocols
Publisher: Humana Press/Springer
Published: 2012
Place of publication: New York
Language: eng
Number of pages: 631
ISBN-13: 9781617799976
Subjects: DNA repair; Laboratory manuals; DNA Repair; Laboratory Manuals
Notes: Includes bibliographical references and index.
Table of contents: title: DNA repair : front and center and not going away!  -- Vilhelm A. Bohr; title: Identification of genes required for damage survival using a cell-based RNAi screen against the Drosophila genome  -- Dashnamoorthy Ravi and Alexander James Roy Bishop; title: Isolating genes involved with genotoxic drug response in the nematode Caenorhabditis elegans using genome-wide RNAi screening  -- Lone Vedel Schøler, Tine Hørning Møller, Steffen Nørgaard, Lotte Vestergaard, and Anders Olesn; title: Establishment of the DNA repair-defective mutants in DT40 cells  -- Masamichi Ishiai, Emi Uchida, and Minoru Takata; title: Electrophoretic mobility shift assays for protein-DNA complexes involved in DNA repair  -- Chun Tsai, Vaughn Smider, Byung Joon Hwang, and Gilbert Chu; title: Comet assay : a sensitive genotoxicity test for the detection of DNA damage and repair  -- Günter Speit and Andreas Rothfuss; title: Detection of DNA damage by comet fluorescence in situ hybridization  -- Wiebke Schlörmann and Michael Glei; title: Quantitative DNA damage and repair measurement  with the yeast comet assay  -- Rui Oliveira and Björn Johansson; title: Analysis of DNA damage and repair in nuclear and mitochondrial DNA of animal cells using quantitative PCR  -- Amy M. Furda, Amanda Smith Bess, Joel N. Meyer, and Bennett Van Houten; title: In vitro DNA mismatch repair in human cells  -- Liya Gu, Charles M. Ensor, and Guo-Min Li; title: Eukaryotic DNA mismatch repair in vitro  -- Fenghua Yuan, Limin Song, Fengsong Liu, Liya Gu, and Yanbin Zhang; title: Immuno-slot blot assay for detection of UVR-mediated DNA damage  -- Mahsa Karbaschi, Nigel J. Brady, Mark D. Evans, and Marcus S. Cooke; title: Quantification of DNA photoproducts in mammalian cell DNA using  radioimmunoassay  -- Thomas R. Berton and David L. Mitchell; title: Measuring the formation and repair of UV damage at the DNA sequence level by ligation-mediated PCR  -- Ahmad Besaratinia and Gerd P. Pfeifer; title: Construction of plasmids containing site-specific DNA interstrand cross-links for biochemical and cell biological studies  -- Milica Enoiu, The Vinh Ho, David T. Long, Johannes C. Walter, and Orlando D. Schärer; title: Replication-coupled DNA interstrand cross-link repair in Xenopus egg extracts  -- Puck Knipscheer, Markus Räschle, Orlando Schärer, and Johannes C. Walter; title: In vitro base excision repair using mammalian cell extracts  -- Jason L. Parsons and Grigory L. Dianov; title: Using Arabidopsis cell extracts to monitor repair of DNA base damage in vitro  -- Dolores Córdoba-Cañero, Teresa Roldán-Arjona, and Rafael R. Ariza; title: In vitro monitoring of base excision repair in Saccharomyces cerevisiae  -- Simone Moertl and Fred Ahne; title: Mitochondrial base excision repair assays  -- Ricardo Gredilla and Tinna Stevnsner; title: O-hydroxylamine-coupled alkaline gel electrophoresis assay  for the detection and measurement of DNA single-strand breaks  -- April M. Luke and Jun Nakamura; title: DNA double-strand break damage and repair assessed by pulsed-field gel electrophoresis  -- Helen E. Bryant; title: Quantitative amplification of single-stranded DNA  -- Eva-Maria Holstein and David Lydall; title: Simple, non-radioactive measurement of single-stranded DNA  at telomeric, sub-telomeric, and genomic loci in budding yeast  -- James M. Dewar and David Lydall; title: Monitoring DNA recombination initiated by HO endonuclease  -- Neal Sugawara and James E. Haber; title: Monitoring I-SceI-induced double-strand break repair in DT40 Cells  -- David du Plessis and Julian E. Sale; title: I-SceI-based assays to examine distinct repair outcomes of mammalian chromosomal double strand breaks  -- Amanda Gunn and Jeremy M. Stark; title: Studying repair of a single protein-bound nick in vivo using the Flp-nick system  -- Ida Nielsen, Anni H. Andersen, and Lotte Bjergbæk; title: Measurement of diversification in the immunoglobulin light chain gene of DT40 cells  -- Julian E. Sale; title: Live cell microscopy of DNA damage response in Saccharomyces cerevisiae  -- Sonia Silva, Irene Gallina, Nadine Eckert-Boulet, and Michael Lisby; title: Use of gene targeting to study recombination in mammalian cell DNA repair mutants  -- Jennifer J. Rahn, Gerald M. Adair, and Rodney S. Nairn; title: In vitro rejoining of double strand breaks in genomic DNA   -- George Iliakis, Emil Mladenov, and Nge Cheong; title: An in vitro DNA double-strand break repair assay based on end-joining of defined duplex oligonucleotides  -- Kamal Datta, Shubhadeep Purkayastha, Ronald D. Neumann, and Thomas A. Winters; title: Assays of bypass replication of genotoxic lesions in cell-free extracts  -- Nana Nikolaishvili-Feinberg and Marila Cordeiro-Stone; title: Quantitative measurement of translesion DNA synthesis  in mammalian cells  -- Omer Ziv, Noam Diamant, Sigal Shachar, Ayal Hendel, and Zvi Livneh; title: Detection of PCNA modifications in Saccharomyces cerevisiae  -- Adelina A. Davies and Helle D. Ulrich; title: In vitro PCNA modification assays  -- Joanne L. Parker and Helle D. Ulrich; title: Analysis of inhibition of DNA replication in irradiated cells using the SV40 based in vitro assay of DNA replication  -- George Iliakis, Emil Mladenov, Ya Wang, and Hong Yan Wang; title: CHK1 kinase activity assay  -- Hong Yan Wang and Ya Wang; title: Histone H2AX phosphorylation : a marker for DNA damage -- Arishya Sharma, Kamini Singh, and Alexandru Almasan.