Developmental divergence and functional convergence of alpha beta and gamma delta T cell lineages
By: by Taras U. Kreslavskiy
Published: 2009
Language: eng
Number of pages: 141
Description: αβ and γδ T-cells develop in the thymus from a common progenitor. At early stages thymocytes have a CD4 - CD8 - double negative (DN) phenotype. DN thymocytes rearrange TCR genes and express pre-TCR, γδ TCR or, rarely, TCRαβ on their surface. Pre-TCR expressing cells progress to the CD4+CD8+ double positive (DP) stage, whereas thymocytes that have chosen the γδ lineage fate do not become DP. Initially, αβ and γδ T-cell lineages were defined on the basis of TCR expression. It became clear, however, that expression of a TCRγδ can drive some cells to the DP stage and therefore support development of αβ lineage. At present αβ and γδ lineages are defined by whether or not cells progress to the DP stage. We utilized a fate mapping approach to demonstrate that premature expression of TCRαβ likewise is compatible with both αβ and γδ lineage fates. It was shown that TCR signal strength rather than TCR type per se influences lineage development with strong TCR signals favoring γδ and weaker - αβ lineage fate. It was unclear whether TCR signaling directly instructed lineage fate or confirmed the lineage choice made prior to TCR expression. By tracing the fate of single T cell precursors, we show that there is no commitment to either the αβ or γδ lineage before TCR expression and that modulation of TCR signaling in progeny of a single TCR-expressing cell changes lineage commitment.

TCR crosslinking in culture blocked the progression of immature TCRγδ+ thymocytes to the DP stage and led to acquisition of a surface phenotype reminiscent of a subset of non-conventional αβ lineage lymphocytes - NKT-cells. It was shown that NKT-cells depend in their surface phenotype and functional properties on the transcription factor PLZF. We demonstrate here that TCR crosslinking -- possibly mimicking agonist selection - leads to PLZF upregulation in immature TCRγδ+ thymocytes. A subset of ex vivo γδ T-cells required PLZF expression for its NKT-cell-like properties. These results reveal a remarkable plasticity in differentiation programs of αβ and γδ T-cell lineages that initially follow different developmental pathways but later can converge in the transcriptional regulation of similar effector programs.
Notes: "April 2009."

Thesis (Ph.D., Division of Medical Sciences (Immunology)--Harvard University, 2009.

Includes bibliographical references.