Regulation of neural migration by direct-current electric fields.
Published: 2006
Language: eng
Number of pages: 160
ISBN-13: 9780494213681
Description: Neuronal migration is a pivotal process during the course of brain development. The role of electrical fields in biological systems is well established, with several cell types exhibiting either polarized motion or growth, usually towards the cathode. I set out to expose cultured neurons to electrical fields in vitro, and to assess whether migration patterns are modulated. Microexplants were isolated from the lateral ganglionic eminence of E16 rat brains and visualized by time-lapse imaging. Control cultures exhibited a radial, symmetrical mode of cell migration. Upon exposure to an electric field (10-250 mV/mm), cells reoriented and migrated towards the cathode, exhibiting higher velocities than in control cultures. The extent of enhanced and velocity and cathodal-directed migration was field strength-dependent. Collectively, this study provides novel evidence that electrical fields can guide mammalian neuronal migration, suggesting that electric fields may regulate neuronal migration in the developing mammalian brain.
Notes: Source: Masters Abstracts International, Volume: 45-03, page: 1395.

Thesis (M.Sc.)--University of Toronto, 2006.

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