Role of the accessory proteins tapasin and Bap29/31 in the biogenesis of MHC class I molecules.
Published: 2004
Language: eng
Number of pages: 129
Description: Class I histocompatibility molecules, consisting of a heavy chain, a light chain (beta2-microglobulin) and peptide, are assembled in the endoplasmic reticulum before being exported to the Golgi apparatus and the cell surface where they are surveyed by cytotoxic T cells. Assembly of the heavy/light chain heterodimer with peptide occurs in the peptide loading complex, which consists of the transporter associated with antigen processing, tapasin, ERp57, calnexin and calreticulin. In the first part of this thesis, I have used a mutational approach to investigate the physical organization of the peptide loading complex with a particular emphasis on tapasin. Several residues were identified to be involved in the interaction between H-2 D d and tapasin and mutations at these positions resulted in phenotypes of low surface expression, altered endoplasmic reticulum to Golgi transport and mildly affected peptide loading. These complex observations, analyzed in the context of similar studies performed with HLA-A2 and H-2 Ld  suggest that the organization of the peptide loading complex can vary depending on the specific class I molecule examined. In the second part of this thesis, I investigated later events in the biogenesis of class I molecules, namely their export from the endoplasmic reticulum to the Golgi apparatus. I found that the putative cargo receptor Bap31 binds to two allotypes of mouse class I molecules with the interaction initiated at the time of heavy chain association with beta2-microglobulin and maintained until the class I molecule has left the endoplasmic reticulm. Consistent with an important role in recruiting class I molecules to transport vesicles, I showed that in the absence of Bap31 and its binding partner Bap29, there is a loss of class I colocalization with p137, a component of mammalian COPII coats. This observation is also associated with a delay in class I traffic from the endoplasmic reticulum to the Golgi. These observations are consistent with the view that class I molecules are largely recruited to endoplasmic reticulum exit sites by Bap29/31 and that Bap29/31 is a cargo receptor for major histocompatibility complex class I molecules.
Notes: Adviser: David B. Williams.

Thesis (Ph.D.)--University of Toronto, 2004.

Electronic version licensed for access by U. of T. users.

Source: Dissertation Abstracts International, Volume: 65-10, Section: B, page: 5060.