Epiblast stem cells
Subtitle: methods and protocols
By: edited by Ramiro Alberio
Series: Methods in molecular biology -- 1074; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- 1074.; Springer protocols (Series)
Publisher: Humana Press
Published: 2013
Place of publication: New York
Language: eng
Number of pages: 216
ISBN-13: 9781627036276; 9781627036283
Subjects: Embryonic stem cells; Research; Embryonic Stem Cells; Laboratory Manuals; Germ Layers; Cytology
Description: In Epiblast Stem Cells: Methods and Protocols, expect researchers in the field provide a detailed collection of techniques and protocols useful to the study of the biology of the pluripotent epiblast. These include methods and techniques used to study epiblast development in different amniotes. This collection brings together contributions from the fields of embryology, stem cell biology and developmental biology together, providing a single volume with detailed procedures for the isolation and culture of epiblasts at different stages of development, and techniques for the study of differentiation into specific lineages.
Notes: Includes bibliographical references and index.
Table of contents: title: Generation and characterization of epiblast stem cells from blastocyst-stage mouse embryos / Daniel C. Factor, Fadi J. Najm, and Paul J. Tesar; title: Reversion of mouse postimplantation epiblast stem cells to a naïve pluripotent state by modulation of signalling pathways / Astrid Gillich, Siqin Bao, and M. Azim Surani; title: Converting mouse epiblast stem cells into mouse embryonic stem cells using small molecules / Wenlin Li and Sheng Ding; title: Isolation and culture of rabbit embryonic stem cells / Arata Honda; title: Goat embryonic stem-like cell derivation and characterization / Esmail Behboodi ... [et al.]; title: Derivation and culture of canine embryonic stem cells / Ian C. Tobias ... [et al.]; title: Isolation and culture pf porcine embryonic stem cells / Ivan Vassiliev and Mark B. Nottle; title: Isolation and culture of pig epiblast stem cells / Aida Rodriguez, David A. Contreras, and Ramiro Alberio; title: Isolation and culture of bovine embryonic stm cells / Shanbo Cao, Fang Wang, and Lin Liu; title: Analysis of molecular markers for staging peri-gastrulating bovine embryos / Isabelle Hue, Severine A. Degrelle, and Christoph Viebahn; title: Chicken embryonic stem cells : establishment and characterization / Pauline Aubel and Bertrand Pain; title: Manipulating the avian epiblast and epiblast-derived stem cells / Cantas Alev ... [et al.]; title: Stepwise differentiation from naïve state pluripotent stem cells to functional primordial germ cells through an epiblast-like state / Katsuhiko Hayashi and Mitinori Saitou; title: Isolation and culture of porcine neural progenitor cells from embryos and pluripotent stem cells / Mikkel A. Rasmussen, Vanessa J. Hall, and Poul Hyttel; title: Derivation of neural precursors from bovine preimplantation embryos / Giovanna Lazzari, Silvia Colleoni, and Cesare Galli.