Cyclooxygenases
Subtitle: methods and protocols
By: edited by Samir S. Ayoub, Roderick J. Flower, Michael P. Seed
Series: Methods in molecular biology -- 644; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 644.; Springer protocols
Publisher: Humana Press; Springer Science+Business Media
Published: 2010
Place of publication: New York, N.Y
Language: eng
Number of pages: 218
ISBN-13: 9781588299536; 9781597453646
Subjects: Laboratory Manuals; Prostaglandin-Endoperoxide Synthases; Cyclooxygenase Inhibitors; Cyclooxygenases; Cyclooxygenase 1; Cyclooxygenase 2
Notes: Includes bibliographical references and index.
Table of contents: title: Part I. In vitro protocols for studying expression and activity of cyclooxygenase enzymes and characterisation of their inhibition patterns; title: 1. In vitro cyclo-oxygenase expression and activity protocols : introduction to Part I / Roderick J. Flower; title: 2. Techniques used to study regulation of cyclooxygenase-2 promoter sites / Hiroyasu Inoue and Rieko Nakata; title: 3. Purification of recombinant human COX-1 and COX-2 / James K. Gierse; title: 4. Cloning and expression of cyclooxygenase-1 and cyclooxygenase-2 / Nicholas R. Staten and Beverly A. Reitz; title: 5. Expression of cyclooxygenase isoforms in the baculovirus expression system / John C. Hunter, Natalie M. Myres, and Daniel L. Simmons; title: 6. Peroxidase active site activity assay / Kelsey C. Duggan, Joel Musee, and Lawrence J. Marnett; title: 7. Study of inhibitors of the PGH synthases for which potency Is regulated by the redox state of the enzymes / Olivier Boutaud and John A. Oates; title: 8. Different methods for testing potential cyclooxygenase-1 and cyclooxygenase-2 inhibitors / Stefan Laufer and Sabine Luik; title: 9. In vitro cyclooxygenase activity assay in tissue homogenates / Samir S. Ayoub; title: 10. Techniques used to characterize the binding of cyclooxygenase Inhibitors to the cyclooxygenase active site / William F. Hood; title: 11. In vitro Assays for cyclooxygenase activity and inhibitor characterization / Mark C. Walker and James K. Gierse; title: Part II. Extraction and measurement of prostanoids and isoprostanes; title: 12. Extraction and measurement of prostanoids and isoprostanes : introduction to Part II / Paola Patrignani; title: 13. Prostanoid extraction and measurement / Lorenzo Polenzani and Samir S. Ayoub; title: 14. Measurement of 8-iso-prostaglandin F2a in biological fluids as a measure of lipid peroxidation / Stefania Tacconelli, Marta L. Capone, and Paola Patrignani; title: Part III. In vivo models to study involvement of cyclooxygenase products in health and disease; title: 15. In vivo models to study cyclooxygenase products in health and disease: introduction to Part III  / Derek W. Gilroy, Melanie Stables, and Justine Newson; title: 16. Protocols to assess the gastrointestinal side effects resulting from inhibition of Cyclo-Oxygenase isoforms / Brendan J.R. Whittle; title: 17. Cyclooxygenase enzymes and their products in the carrageenan-induced pleurisy in rats / Adrian R. Moore, Samir S. Ayoub, and Michael P. Seed; title: 18. Iloprost-induced nociception : determination of the site of anti-nociceptive action of cyclooxygenase inhibitors and the Involvement of cyclooxygenase products in central mechanisms of nociception / Samir S. Ayoub and Regina M. Botting.