Phosphodiesterase methods and protocols
By: edited by Claire Lugnier
Contributors: Lugnier, Claire
Series: Methods in molecular biology -- v. 307; Methods in molecular biology (Clifton, N.J.) --  307
Publisher: Humana Press; Humana
Published: 2005
Place of publication: Totowa, N.J
Language: eng
Number of pages: 324
Subjects: Phosphodiesterases -- Laboratory manuals
Genres: Laboratory manuals
Notes: Includes bibliographical references and index
Table of contents: title: Study of cAMP microdomains in cells / Marco Mongillo ... [et al.]; title: High-resolution measurements of cAMP signals in three-dimensional microdomains / Jeffrey W. Karpen and Thomas C. Rich; title: Cygnets: in vivo characterization of novel cGMP indicators and in vivo imaging of intracellular cGMP / Akira Honda ... [et al.]; title: High-throughput screening of PDE activity in living cells / Thomas C. Rich and Jeffrey W. Karpen; title: Assessment of PDE isozyme contribution in cell and tissue extracts / Thäråse Keravis, Rima Thaseldar-Roumiä, and Claire Lugnier; title: Localization of the cGMP-hydrolysing phosphodiesterase type 9 in the rat brain by a non-radioactive in situ hybridization / Wilma C.G. Van Staveren and Marjanne Markerink-Van Ittersum; title: Determination of Ca2+/calmodulin-stimulated phosphodiesterase activity in intact cells / C. Yan; title: Adenovirus mediated overexpression of murine cyclic nucleotide phosphodiesterase 3B (mPDE3B) / Faiyaz Ahmad ... [et al.]; title: Identification of promoter elements in the 5'-flanking region of the murine cyclic nucleotide phosphodiesterase 3B gene / Hanguan Liu ... [et al.]; title: Purification of PDE6 isozymes from mammalian retina / Dana C. Pentia ... [et al.]; title: cGMP binding to the regulatory GAF domains of photoreceptor phosphodiesterase / Rick H. Cote; title: Renaturation of the catalytic domain of PDE4A expressed in escherichia coli as inclusion bodies / Wito Richter, Thomas Hermsdorf, and Dietrich Dettmer; title: Determining the subunit structure of phosphodiesterases using gel-filtration and sucrose density gradient centrifugation / Wito Richter; title: Crystallization of cyclic nucleotide phosphodiesterases / Hengming Ke, Qing Huai, and Robert X. Xu; title: Generation of PDE4 knockout mice by gene targeting / S. L. Catherine Jin, Anne M. Latour, and Marco Conti; title: Immunoprecipitating PDE2 phosphorylated and inactivated by associated protein kinase / J. Kelley Bentley; title: Methods to investigate ERK MAP kinase phosphorylation and regulation of activity of PDE4 cyclicamp-specific phosphodiesterases / Elaine V. Hill, Miles D. Houslay, and George S. Baillie; title: R; title: Radiolabeled ligand binding to the catalytic sites or allosteric sites of PDE5 and PDE11 / James L. Weeks ... [et al.]; title: Analysis of dimerization determinants of the PDE6 catalytic subunits / Khakim G. Muradov, Kimberley K. Boyd, and Nikolai O. Artemyev; title: Interaction between catalytic and inhibitory subunits of PDE6 / Nikolai O. Artemyev; title: Purification, reconstitution on lipid vesciles and assays of PDE6 and its activator G protein, transducin; title: Theodore G. Wensel, Feng He, and Justine A. Malinski