Focus on Multidimensional Microscopy
Contributors: Philip C. Cheng (Editor); P. P. Hwang (Editor); J. L. Wu (Editor); G. Wang (Editor); H. Kim (Editor)
Publisher: World Scientific Pub Co Inc
Published: December 1999
Language: eng
Format: Hardcover
Number of pages: 320
ISBN-13: 9789810239916
Subjects: Microscopy; Science/Mathematics; Science; Microscopes & Microscopy; General
First sentence: To use the fluorescence lifetime as a contrast parameter for microscopy instead of the fluorescent intensity has a number of advantages: (i) The fluorescence lifetime of a probe molecule often differs considerably from that of the background radiation or autofluorescence, providing an opportunity to suppress the contributions of the latter to the total signal; (ii) Fluorescence lifetime imaging provides an alternative to the technique of ratio imaging based on wavelength differences which is used for e.g. the imaging of ion concentrations in biological samples.