Heterologous gene expression in E. coli
Subtitle: methods and protocols
By: edited by Thomas C. Evans, Jr., Ming-Qun Xu
Series: Springer protocols; Methods in molecular biology -- 705; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 705.
Publisher: Humana Press
Published: 2011
Place of publication: New York, NY
Language: eng
Number of pages: 310
ISBN-13: 9781617379666; 9781617379673
Subjects: Laboratory manuals; Genetics; Gene expression; Gene Expression; Laboratory Manuals; Escherichia coli
Notes: Includes bibliographical references and index.

Also available online.
Table of contents: title: Adjustment of codon usage frequencies by codon harmonization improves protein expression and folding / Evelina Angov, Patricia M. Legler, and Ryan M. Mease; title: SUMO fusion technology for enhanced protein expression and purification in prokaryotes and eukaryotes / Raymond J. Peroutka III, Steven J. Orcutt, James E. Strickler, and Tauseef R. Butt; title: Molecular and chemical chaperones for improving the yields of soluble recombinant proteins / Ario de Marco; title: Genetic selection of solubility-enhanced proteins using the twin-arginine translocation system / Adam C. Fisher, Mark A. Rocco, and Matthew P. DeLisa; title: Protein folding liquid chromatography / Quan Bai and Xindu Geng; title: Site-specific protein labeling by intein-mediated protein ligation / Inca Ghosh, Nancy Considine, Elissa Maunus, Luo Sun, Aihua Zhang, John Buswell, Thomas C. Evans, Jr., and Ming-Qun Xu; title: Efficient expression of human aromatase (CYP19) in E. coli / Norio Kagawa; title: Expression of recombinant cytochromes c in E. coli / Yuri Y. Londer; title: Semi-synthesis of glycoprotiens from E. coli through native chemical ligation / Jonathan P. Richardson and Derek Macmillan; title: Expression of recombinant proteins with uniform N-termini / Orsolya Király, Lan Guan, and Miklós Sahin-Tóth; title: Recent developments in difficult protein expression : a guide to E. coli strains, promoters, and relevant host mutations / James C. Samuelson; title: Periplasmic chaperones used to enhance functional secretion of proteins in E. coli / Martin Schlapschy and Arne Skerra; title: Engineering unusual amino acids into peptides using lantibiotic synthetase / Jun-ichi Nagao, Kouki Shioya, Yoshitaka Harada, Ken-ichi Okuda, Takeshi Zendo, Jiro Nakayama, and Kenji Sonomoto; title: The targeted expression of nucleotide sugar transporters to the E. coli inner membrane / Joe Tiralongo and Andrea Maggioni; title: Detection of protein-protein interactions in bacteria by GFP-fragment reconstitution / Akira Kanno, Takeaki Ozawa, and Yoshio Umezawa; title: Enhancing the solubility of recombinant proteins in Escherichia coli by using hexahistidine-tagged maltose-binding protein as a fusion partner / Ping Sun, Joseph E. Tropea, and David S. Waugh; title: Introducing predetermined mutations throughout a target gene using TDEM (Transposon-Directed Base-Exchange Mutagenesis) / Yun Cheol Kim; title: Fluorescent site-specific labeling of Escherichia coli expressed proteins with Sfp phosphopantetheinyl transferase / Aihua Zhang, Luo Sun, John Buswell, Nancy Considine, Inca Ghosh, Anastasiya Masharina, Christopher Noren, and Ming-Qun Xu.