Exploring the Plasticity of Cellular Fate Using Defined-Factor Reprogramming
Contributors: Eggan, Kevin; Engert, Florian; Dulac, Catherine; Meissner, Alexander
Published: 2012
Language: eng
Description: Cellular fate, once established, is usually stable for the lifetime of the cell. However, the mechanisms that restrict the developmental potential of differentiated cells are in principle reversible, as demonstrated by the success of animal cloning from a somatic genome through somatic cell nuclear transfer (SCNT). An increased understanding of the molecular determinants of cell fate has also enabled the reprogramming of cell fate using defined transcription factors; recently, these efforts have culminated in the discovery of four genes that convert somatic cells into induced pluripotent stem cells (iPSCs), which resemble embryonic stem cells (ESCs) and can give rise to all the cell types in the body.
Notes: Keywords: Induced pluripotent stem cell, Motor neuron, Regeneration, Reprogramming, TGF-beta, Transdifferentiation.

Thesis Ph.D. Harvard University 2012