Reverse chemical genetics
Subtitle: methods and protocols
By: edited by Hisashi Koga
Series: Springer protocols; Methods in molecular biology -- 577
Publisher: Humana Press
Published: 2009
Place of publication: New York, NY
Language: eng
Number of pages: 272
ISBN-13: 9781607612315
Subjects: Genetic Techniques; Combinatorial Chemistry Techniques; Methods; Molecular Biology; Biochemical genetics; Laboratory Manuals
Notes: Includes bibliographical references and index.

Also available online.

Mode of access: Internet.
Table of contents: title: ORFeome cloning / Osamu Ohara; title: Systematic cloning of an ORFeome using the gateway system / Akihisa Matsuyama and Minoru Yoshida; title: High-throughput construction of ORF clones for production of the recombinant proteins / Hisashi Yamakawa; title: Computational overview of GPCR gene universe to support reverse chemical genomics study / Makiko Suwa and Yukiteru Ono; title: High-performance gene expression module analysis tool and its application to chemical toxicity data / Wataru Fujibuchi ... [et al.]; title: Human protein reference database and human proteinpedia as discovery tools for systems biology / T.S. Keshava Prasad, Kumaran Kandasamy, and Akhilesh Pandey; title: High-throughput production of the recombinant proteins expressed in Escherichia coli utilizing cDNA resources / Kiyo Shimada and Hisashi Koga; title: An insect cell-free system for recombinant protein expresion using cDNA resources / Takashi Syuzuki ... [et al.]; title: ^; title: Recombinant protein production by a kaiko-baculovirus system / Hidekazu Nagaya; title: Pulse-chase experiment for the analysis of protein stability in cultured mammalian cells by covalent fluorescent labeling of fusion proteins / Kei Yamaguchi ... [et al.]; title: Application of 2D-DIGE in cancer proteomics toward personalized medicine / Tadashi Kondo and Setsuo Hirohashi; title: Fully automated two-dimensional electrophoresis system for high-throughput protein analysis / Atsunori Hiratsuka and Kenji Yokoyama; title: Large-scale whole mount in situ hybridization of mouse embryos / Hirohito Shimizu, Kenta Uchibe, and Hiroshi Asahara; title: Identification of the specific binding proteins of bioactive small coumpound using affinity resins / Akito Tanaka; title: Protein microarrays : effective tools for the study of inflammatory diseases / Xiaobo Yu ... [et al.]; title: Preparation of highly sensitive protein array using reactive polymer / Toshifumi Shiroya ... [et al.]; title: ^; title: ^^; title: High-throughput SPR biosensor / Motoki Kyo ... [et al.]; title: Drug discovery through functional screening in the Drosophila heart / Takeshi Akasaka and Karen Ocorr; title: Permeable cell assay : a method for high-throughput measurement of cellular ATP synthetic activity / Kiyotaka Y. Hara; title: Novel methodology for immobilization of biomolecules on the surface of a photoresponsible polymer containing azobenzene moiety / Osamu Watanabe.; title: ^^