Hematopoietic stem cell protocols
By: edited by Kevin D. Bunting.
Contributors: Bunting, Kevin D.
Edition: 2nd ed.
Publisher: Humana; Humana Press
Published: 2008
Place of publication: Totowa, N.J
Language: eng
Number of pages: 312
ISBN-13: 9781588298683
Subjects: Hematopoietic stem cells; Genetic engineering; Hematopoietic Stem Cells -- cytology -- Laboratory Manuals; Cytological Techniques -- methods -- Laboratory Manuals; Hematopoietic Stem Cells -- physiology -- Laboratory Manuals
Genres: Laboratory Manuals.
Description: With new technologies emerging and old technologies being reimagined, the field of hematopoietic stem cell (HSC) research continues to grow rapidly in pursuit of improved therapies for many blood diseases. A team of researchers supply this volume with in-depth, readily reproducible methods for effective characterization of HSC and their developmental potential. An introductory chapter also hints at potential techniques that may develop in the future. Each protocol offers step-by-step laboratory instructions, lists of the necessary equipment, and tips on troubleshooting and avoiding known pitfalls.
Notes: Previous ed. : 2002.

Includes bibliographical references and index.
Table of contents: title: The expanding tool kit for hematopoietic stem cell research / William Tse and Kevin D. Bunting; title: Isolation of quiescent murine hematopoietic stem cells by homing properties / Tarja A. Juopperi and Saul J. Sharkis; title: Mobilization of hematopoietic stem and progenitor cells in mice / Simon N. Robinson and Ronald P. van Os; title: Transmigration of human CD34⁺ cells / Seiji Fukuda and Louis M. Pelus; title: Flow cytometry-based cell cycle measurement of mouse hematopoietic stem and progenitor cells / Hongmei Shen, Matthew Boyer, and Tao Cheng; title: Analysis of apoptosis in hematopoietic stem cells by flow cytometry / William G. Kerr; title: In vitro hematopoietic differentiation of murine embryonic stem cells / Jinhua Shen and Cheng-Kui Qu; title: Hematopoietic development of human embryonic stem cells in culture / Xinghui Tian and Dan S. Kaufman; title: In vitro human T cell development directed by notch-ligand interactions / Génève Awong, Ross N. La Motte-Mohs, and Juan Carlos Zuñiga-Pflücker; title: In vitro assays for cobblestone area-forming cells, LTC-IC, and CFU-C / Ronald P. van Os, Bertien Dethmers-Ausema, and Gerald de Haan; title: Hematopoietic stem cell transplant in mice by intra-femoral injection / Yuxia Zhan and Yi Zhao; title: Hematopoietic stem cell transplant into non-myeloblated W/Wv mice to detect steady-state engraftment defects / Zhengqi Wang and Kevin D. Bunting; title: Isolation and functional characterization of side population stem cells / Jonathan B. Johnnidis and Fernando D. Camargo; title: Transplanatation of chimeric fetal liver to study hematopoiesis / Sigrid Eckardt and K. John McLaughlin; title: Immunodeficient mouse models to study human stem cell-mediated tissue repair / Ping Zhou ... [et al.]; title: Retroviral transduction of murine hematopoietic stem cells / Peter Haviernik, Yi Zhang, and Kevin D. Bunting; title: Lentiviral gene transduction of mouse and human stem cells / Zhaohui Ye, Xiaobing Yu, and Linzhao Cheng; title: Retroviral integration site analysis in hematopoietic stem cells / Olga S. Kustikova, Christopher Baum, and Boris Fehse; title: A detailed protocol for bacterial artificial chromosome recombineering to study essential genes in stem cells / Andriy Tsyrulnyk and Richard Moriggl; title: Bioluminescence imaging of hematopoietic stem cell repopulation in murine models / Yuan Lin ... [et al.]