Directed Evolution of Sortase Activity and Specificity
Contributors: Liu, David R.; Saghatelian, Alan; Chaikof, Elliot
Published: 2014
Language: eng
Description: Nature employs complex networks of protein-tailoring enzymes to effect the post-translational modification of proteins in vivo. By comparison, modern chemical methods rely upon either nonspecific labeling techniques or upon the genetic incorporation of bioorthogonal handles. To develop truly robust bioconjugates it is necessary to develop methods which possess the exquisite activity and specificity observed in biological catalysts. One attractive strategy to achieve this is the engineering of protein-tailoring enzymes possessing user-defined specificity and high catalytic efficiency.
Notes: Keywords: Biochemistry, Bioconjugation, Chemical Biology, Directed Evolution, Sortase.

Thesis Ph.D. Harvard University 2014