Structural investigation of a blue chromoprotein and its yellow variant from sea anemone.
Published: 2006
Language: eng
Number of pages: 98
ISBN-13: 9780494161555
Description: The green fluorescent protein (GFP) family is comprised of diverse members thus far isolated from various species in Cnidaria. In search of new members in the Cnidopus japonicus species of sea anemone, we identified a non-fluorescent chromoprotein that belongs to the GFP superfamily. This protein named Kaede Green (KGr) appears blue in colour with an absorbante maximum of 610 nm. Replacement of Tyr64 with Leu in wild-type KGr leads to an apparent blue to yellow colour change with a new spectral absorbante maximum of 417 nm. To investigate the correlation between structure and absorbante of KGr and the yellowish mutant, we determined their crystal structures to 1.8 A and 2.0 A, respectively. The KGr chromophore closely resembles the "Kindling Fluorescent Protein" (KFP) and Rtms5 in that the five-membered heterocyclic ring adopts a trans non co-planar chromophore. The yellow mutant contains a small chromophore due to the lack of the hydroxyphenyl ring from Tyr64. These results help our understanding of KGr and other members of the GFP family.
Notes: Source: Masters Abstracts International, Volume: 44-06, page: 2726.

Thesis (M.Sc.)--University of Toronto, 2006.

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