Generation of cDNA libraries
Subtitle: methods and protocols
By: edited by Shao-Yao Ying
Contributors: Ying, Shao-Yao
Series: Methods in molecular biology --  221; Methods in Molecular Biology (Clifton, N.J.) -- 221
Publisher: Humana Press
Published: 2003
Place of publication: Totowa, N.J
Language: eng
Number of pages: 335
Subjects: Gene libraries -- Laboratory manuals; Antisense DNA -- Laboratory manuals
Genres: Laboratory manuals
Notes: Includes bibliographical references and index
Table of contents: title: Complementary DNA libraries: an overview / Shao-Yao Ying; title: Rapid amplification of cDNA ends / Yue Zhang; title: cDNA generation on paramagnetic beads / Zhaohui Wang and Michael G.K. Jones; title: Construction of a normalized cDNA library by mRNA-cDNA hybridization and subtraction / Ye-Guang Chen; title: Amplification of cDNA ends using PCR suppression effect and step-out PCR / Mikhail V. Matz ... [et al.]; title: Use of inverse PCR to clone cDNA ends / Sheng-He Huang, Steven H.M. Chen, and Ambrose Y. Jong; title: Construction of size-fractionated cDNA library assisted by an in vitro recombination reaction / Osamu Ohara; title: Construction of a full-length enriched and 5'-end enriched cDNA library using the oligo-capping method / Yutaka Suzuki and Sumio Sugano; title: cDNA library construction using in vitro transcriptional amplification / Shi-Lung Lin and Henry Ji; title: Amplification of representative cDNA pools from microscopic amounts of animal tissue / Mikhail V. Matz; title: Single cell cDNA library construction using cycling aRNA amplification / Shi-Lung Lin; title: mRNA/cDNA library construction using RNA-polymerase cycling reaction / Shi-Lung Lin and Shao-Yao Ying; title: Quality assessment of cDNA libraries / Hans-Jürgen Fülle; title: Assessment of the quality of mRNA libraries by agarose gel electrophoresis / Tsen-Yin Lin and Shao-Yao Ying; title: PACS RT-PCR: a method for the generation and measurement of any poly(A)-containing mRNA not affected by contaminating genomic DNA / Igor Nepluev and Rodney J. Folz; title: Single-cell mRNA library analysis by northern blot hybridization / Shi-Lung Lin; title: Generation of cDNA libraries for profiling gene expression of given tissues or cells / Xin Zhang, Qiu-Hua Huang, and Ze-Guang Han; title: Screening poly (dA/dT(-)) cDNA for gene identification / San Ming Wang ... [et al.]; title: Generation of longer cDNA fragments from SAGE tags for gene identification / Jian-Jun Chen ... [et al.]; title: Generation of full-length cDNA libraries enriched for differentiatlly expressed genes / Bakhyt Zhumabayeva ... [et al.]; title: Subtractive hybridization for the identification of differentially expressed genes using uracil-DNA glycosylase and mung-bean nuclease / Tsen-Yin Lin and Shao-Yao Ying; title: Subtractive cloning of differential genes using RNA-PCR / Shao-Yao Ying and Shi-Lung Lin; title: Strategy for construction of a cDNA encoding a repetitive amino acid sequence / Masahiro Asada and Toru Imamura; title: Preparing lambda libraries for expression of proteins in prokaryotes or eukaryotes / Rebecca L. Mullinax and Joseph A. Sorge; title: Peptide library construction from RNA-PCR-derived RNAs / Shi-Lung Lin; title: Identifying interacting proteins in an Escherichia coli-based two-hybrid system / Bonnie Wu, Rebecca L. Mullinax, and Joseph A. Sorge; title: Future perspectives / Shao-Yao Ying