Riboswitches
Subtitle: methods and protocols
By: edited by Alexander Serganov
Series: Methods in molecular biology -- v. 540; Springer protocols
Publisher: Humana
Published: 2009
Place of publication: New York, NY
Language: eng
Number of pages: 366
ISBN-13: 9781934115886; 9781597455589
Subjects: Laboratory Techniques and Procedures; Ligands (Biochemistry); RNA; Messenger RNA; Gene Expression Regulation; Chemistry; Laboratory Manuals; Physiology; RNA-protein interactions
Notes: Includes bibliographical references and index.
Table of contents: title: Predicting riboswitch regulation on a genomic scale / J.E. Barrick; title: Enzymatic ligation strategies for the preparation of purine riboswitches with site-specific chemical modifications / R. Rieder, C. Hobartner and R. Micura; title: Application of fluorescent measurements for characterization of riboswitch-ligand interactions / B. Heppell ... [et al.]; title: Transcriptional approaches to riboswitch studies / A. Mironov, V. Epshtein and E. Nudler; title: Kinetics of riboswitch regulation studied by in vitro transcription / J.K. Wickiser; title: Molecular basis of RNA-mediated gene regulation on the adenine riboswitch by single-molecule approaches / J.F. Lemay ... [et al.]; title: Methods for analysis of ligand-induced RNA conformational changes / C.A. Brautigam, C.A. Wakeman and W.C. Winkler; title: Monitoring RNA-ligand interactions using isothermal titration calorimetry / S.D. Gilbert and R.T. Batey; title: Preparation and crystallization of riboswitch-ligand complexes / O. Pikovskaya ... [et al.]; title: ^; title: Crystallization of the glmS ribozyme-riboswitch / D.J. Klein and A.R. Ferre-D'Amare; title: Riboswitch conformations revealed by small-angle X-ray scattering / J. Lipfert, D. Herschlag and S. Doniach; title: Time-resolved NMR spectroscopy: ligand-induced refolding of riboswitches / J. Buck ... [et al.]; title: Analysis of the RNA backbone: structural analysis of riboswitches by in-line probing and selective 2'-hydroxyl acylation and primer extension / C.A. Wakeman and W.C. Winkler; title: Identification of metabolite-riboswitch interactions using nucleotide analog interference mapping and suppression / J.K. Soukup and G.A. Soukup; title: RNA-dependent RNA switches in bacteria / T.M. Henkin; title: Probing mRNA structure and sRNA-mRNA interactions in bacteria using enzymes and lead(II) / C. Chevalier ... [et al.]; title: Structural probing of RNA thermosensors / C. Chiaruttini, F. Allem and M. Springer; title: ^; title: ^^; title: Ribosomal initiation complexes probed by toeprinting and effect of trans-acting translational regulators in bacteria / P. Fechter ... [et al.]; title: Isolation and characterization of the heat shock RNA 1 / I. Shamovsky and E. Nudler; title: Analysis of tRNA-directed transcription antitermination in the T box system in vivo / T.M. Henkin; title: In vitro selection of conformational probes for riboswitches / G. Mayer and M. Famulok; title: A green fluorescent protein (GFP)-based plasmid system to study post-transcriptional control of gene expression in vivo / J.H. Urban and J. Vogel; title: High-throughput screens to discover synthetic riboswitches / S.A. Lynch, S. Topp and J.P. Gallivan; title: A mammalian cell-based assay for screening inhibitors of RNA cleavage / L. Yen, B.R. Stockwell and R.C. Mulligan; title: In vitro selection of glmS ribozymes / K.H. Link and R.R. Breaker.; title: ^^