Assessing the role of mast cells in cardiac contractile dysfunction following hemorrhagic shock and resuscitation.
Published: 2004
Language: eng
Number of pages: 106
Description: Hemorrhagic shock (HS) is associated with significant myocardial contractile dysfunction. We investigated the role of cardiac mast cell degranulation in contractile dysfunction following HS using a rat model of HS. HS was induced by the removal of blood from the carotid artery until a MAP of 40mmHg was established and maintained for 60 minutes. Animals were resuscitated with their shed blood. 15 minutes prior to HS, animals received either saline, or mast cell stabilizing agents, ketotifen or cromolyn. HS resulted in an increased mast cell density in the heart and the induction of cardiac IL-1beta and IL-6 mRNA. Mast cell stabilization significantly improved cardiac function and attenuated the HS-induced rise in cardiac IL-1beta and IL-6 mRNA. Elevated serum beta-hexosaminidase during HS provided a marker of degranulation. These results suggest that mast cells and their granule mediators may contribute to the cardiac contractile dysfunction that occurs following HS/R.
Notes: Advisers:  Alex Romaschin; Tom Lindsay.

Thesis (M.Sc.)--University of Toronto, 2004.

Electronic version licensed for access by U. of T. users.

Source: Masters Abstracts International, Volume: 43-03, page: 0764.

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