Chaperonin protocols
By: edited by Christine Schneider
Contributors: Schneider, Christine, 1942-
Series: Methods in molecular biology -- 140; Methods in molecular biology (Clifton, N.J.) --  140
Publisher: Humana Press
Published: 2000
Place of publication: Totowa, N.J
Language: eng
Number of pages: 212
Subjects: Molecular chaperones -- Purification -- Laboratory manuals
Genres: Laboratory manuals
Description: "In Chaperonin Protocols, Christine Schneider has assembled a unique collection of readily reproducible protocols for the study of chaperonins, intracellular proteins critical to many biological processes. Written by experienced investigators who have successfully honed their methods to a fineness, the protocols focus on the purification of chaperonins from different species along with their corresponding cofactors, and on chaperonin activity assays for in vivo as well as in vitro work. Many activity assays are given for GroEL, which can also be applied to mitochrondrial Hsp60. There are also assays for the eukaryotic chaperonin TRiC and handy methods--for example, one for preparing labeled probes--that can be used for various purposes and prove helpful in numerous different procedures." "Critically important to a greater understanding of such disorders as cystic fibrosis, Alzheimer's disease, and BSE, Chaperonin Protocols offers both novice and experienced investigators fast access to today's best and most productive chaperonin methods, all explained in step-by-step detail to ensure robust and reproducible results. Book jacket."--Jacket.
Notes: Includes bibliographical references and index