Establishing a superior tetracycline-inducible gene expression system for mouse CD200, a potential therapeutic immunosuppressive molecule.
Published: 2007
Language: eng
Number of pages: 144
ISBN-13: 9780494273739
Description: CD200 (originally called OX2) is a membrane glycoprotein expressed by a variety of cell types, including lymphocytes, neurons and endothelium. It is the ligand for CD200 receptors (CD200Rs) whose expression is limited to hematopoietic cells, especially myeloid cells. A number of in vivo  studies have implied that the interaction of CD200 with CD200R1 may inhibit a variety of immune responses, including graft rejection, autoimmune disease, fetal loss syndrome, and cancer.To study further the biological function of CD200 and the CD200Rs, our lab produced TRE-CD200-EGFP transgenic mice using a Tet-On inducible system. This thesis evaluated the inducible function of rtTA2s-M2, a new generation of Tet-On regulator, and compared it to the old version of rtTA in vitro and in vivo. The data suggest that induction of the CD200-EGFP transgene in rtTA2s-M2 transgenic mice is superior to that achieved in rtTA transgenic mice following doxycycline feeding in vivo.
Notes: Source: Masters Abstracts International, Volume: 45-06, page: 3122.

Thesis (M.Sc.)--University of Toronto, 2007.

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