Chemotaxis
Subtitle: methods and protocols
By: edited by Tian Jin and Dale Hereld
Series: Methods in molecular biology -- 571; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 571.; Springer protocols
Publisher: Humana Press
Published: 2009
Place of publication: New York, N.Y
Language: eng
Number of pages: 537
ISBN-13: 9781607611974; 9781607611981
Subjects: Laboratory manuals; Chemotaxis; Laboratory Manuals; Chimiotaxie
Description: "Fundamental to the development and vital functions of organisms, the migration of motile cells due to the detection of shallow gradients of specific chemical signals in their environments, or chemotaxis, can be clearly seen as a major force in cell biology. In Chemotaxis: Methods and Protocols, expert researchers in the field provide state-of-the-art methods for investigating cell migration behaviors, studying molecular components involved in detecting extracellular signals and directing cell movement, visualizing spatiotemporal dynamics of the components in signaling networks of chemotaxis in real time, and constructing quantitative models that simulate chemoattractant-induced cell responses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls." "Comprehensive and cutting-edge, Chemotaxis: Methods and Protocols serves scientists with practical guidance on the diverse methodologies that are currently propelling chemotaxis research forward in order to further our understanding of this vital biological system."--BOOK JACKET.
Notes: Includes bibliographical references and index.

Also issued online.
Table of contents: title: 1. Microfluidic techniques for the analysis of bacterial chemotaxis / Derek L. Englert, Arul Jayaraman, and Michael D. Manson; title: 2. Prokaryotic phototaxis / Wouter D. Hoff ... [et al.]; title: 3. Photoorientation in photosynthetic Flagellates / Donat-Peter Häder and Michael Lebert; title: 4. Dictyostelium slug phototaxis / Sarah J. Annesley and Paul R. Fisher; title: - 5. Electrotaxis and wound healing: experimental methods to study electric fields as a directional signal for cell migration / Guangping Tai ... [et al.]; title: 6. Chemotropism during yeast mating / Peter J. Follette and Robert A. Arkowitz; title: 7. Group migration and signal relay in Dictyostelium / Paul W. Kriebel and Carole A. Parent; title: 8. Quantitative analysis of distal tip cell migration in C. elegans / Myeongwoo Lee and Erin J. Cram; title: 9. Inflammation and wound healing in Drosophila / Brian Stramer and Will Wood; title: 10. Neutrophil motility in vivo using zebrafish / Jonathan R. Mathias, Kevin B. Walters, and Anna Huttenlocher; title: 11. Chemotaxis in neutrophil-like HL-60 cells / Arthur Millius and Orion D. Weiner; title: 12. Chemokine receptor dimerization and chemotaxis / José Miguel Rodríguez-Frade ... [et al.]; title: 13. Intravital two-photon imaging of adoptively transferred B lymphocytes in inguinal lymph nodes / Chung Park, Il-Young Hwang, and John H. Kehrl; title: 14. Breast cancer cell movement: imaging invadopodia by TIRF and IRM microscopy / Xuehua Xu, Peter Johnson, and Susette C. Mueller; title: 15. In Vivo assay for tumor cell invasion / Lorena Hernandez ... [et al.]; title: 16. Quantitative studies of neuronal chemotaxis in 3D / William J. Rosoff ... [et al.]; title: 17. Assays for chemotaxis and chemoattractant-stimulated TorC2 activation and PKB substrate phosphorylation in Dictyostelium / Yoichiro Kamimura, Ming Tang, and Peter Devreotes; title: 18. Biochemical responses to chemoattractants in Dictyostelium: ligand-receptor interactions and downstream kinase activation / Xin-Hua Liao and Alan R. Kimmel; title: 19. Quantifying in vivo phosphoinositide turnover in chemotactically competent Dictyostelium cells / Nadine Pawolleck and Robin S.B. Williams; title: 20. In Vivo measurements of cytosolic calcium in Dictyostelium discoideum / Claire Y. Allan and Paul R. Fisher; title: 21. Chemokine receptor signaling and HIV infection / Yuntao Wu; title: 22. Spatiotemporal stimulation of single cells using flow photolysis / Carsten Beta; title: 23. Spatiotemporal regulation of Ras-GTPases during chemotaxis / Atsuo T. Sasaki and Richard A. Firtel; title: 24. FRAP analysis of chemosensory components of Dictyostelium / Carrie A. Elzie and Chris Janetopoulos; title: 25. Monitoring dynamic GPCR signaling events using fluorescence microscopy, FRET imaging, and single-molecule imaging / Xuehua Xu, Joseph A. Brzostowski, and Tian Jin; title: 26. Imaging actin cytoskeleton dynamics in Dictyostelium chemotaxis / Günther Gerisch; title: 27. Analysis of actin assembly by in vitro TIRF microscopy / Dennis Breitsprecher ... [et al.]; title: 28. Single-molecule imaging techniques to visualize chemotactic signaling events on the membrane of living Dictyostelium cells / Yukihiro Miyanaga, Satomi Matsuoka, and Masahiro Ueda; title: 29. Imaging B-cell receptor signaling by single-molecule techniques / Pavel Tolar and Tobias Meckel; title: 30. Light microscopy to image and quantify cell movement / Deborah J. Wessels, Spencer Kuhl, and David R. Soll; title: 31. Mathematics of Experimentally generated chemoattractant gradients / Marten Postma and Peter J.M. van Haastert; title: 32. Modeling spatial and temporal dynamics of chemotactic networks / Liu Yang and Pablo A. Iglesias; title: 33. Computational modeling of signaling networks for eukaryotic chemosensing / Martin Meier-Schellersheim, Frederick Klauschen, and Bastian Angermann.