Antibody-drug conjugates
By: edited by Laurent Ducry
Series: Methods in molecular biology -- 1045; Methods in molecular biology (Clifton, N.J.) -- v. 1045.
Publisher: Humana Press; Springer
Published: 2013
Place of publication: New York
Language: eng
Number of pages: 317
ISBN-13: 9781627035408; 9781927035415
Subjects: Immunoglobulins; Antibodies; Pharmaceutical Preparations; Drug Combinations
Description: Antibody-drug conjugates (ADCs) represent a promising therapeutic approach for cancer patients by combining the antigen-targeting specificity of monoclonal antibodies (mAbs) with the cytotoxic potency of chemotherapeutic drugs. In Antibody-Drug Conjugates, expert researchers provide detailed protocols for many of the key ADC techniques necessary for working in the field. These chapters and methodologies are aimed at the key tasks necessary to identify a suitable target, properly design the mAb, the linker and the payload, as well as to conjugate them in a reproducible and scalable fashion. Written in the highly successful Methods in Molecular Biology format, these detailed chapters include the kind of practical implementation advice that guarantees quality results. Authoritative and timely, Antibody-Drug Conjugates aims to further drive ADC development and thus help toward improving cancer treatments of the future.
Notes: Includes bibliographical references and index.
Table of contents: title: Antibody-drug conjugate (ADC) clinical pipeline : a review  -- Ingrid Sassoon and Veronique Blanc; title: Antibody-drug conjugate target selection : critical factors  -- Neil H. Bander; title: Selecting an optimal antibody for antibody-drug conjugate therapy : internalization and intracellular localization  -- Jay Harper ... [et al.]; title: Antibody-drug conjugate payloads  -- Jan Anderl ... [et al.]; title: Linker technologies for antibody-drug conjugates  -- Birte Nolting; title: In vivo testing of drug-linker stability  -- Pierre-Yves Abecassis and Celine Amara; title: Pharmacokinetics and ADME characterizations of antibody-drug conjugates  -- Kedan Lin, Jay Tibbitts, and Ben-Quan Shen; title: Safe handling of cytotoxic compounds in a biopharmaceutical  environment  -- Miriam I. Hensgen and Bernhard Stump; title: Micro- and mid-scale maleimide-based conjugation of cytotoxic drugs to antibody hinge region thiols for tumor targeting  -- James E. Stefano ... [et al.]; title: Protocols for lysine conjugation  -- Marie-Priscille Brun and Laurence Gauzy-Lazo; title: Engineering THIOMABs for site-specific conjugation  of thiol-reactive linkers  -- Sunil Bhakta, Helga Raab, and Jagath R. Junutula; title: Enzymatic antibody modification by bacterial transglutaminase  -- Patrick Dennler, Roger Schibli, and Eliane Fischer; title: Formulation development of antibody-drug conjugates  -- William J. Galush and Aditya A. Wakankar; title: Conjugation process development and scale-up  -- Bernhard Stump and Jessica Steinmann; title: Methods for conjugating antibodies to nanocarriers  -- Anil Wagh and Benedict Law; title: Drug-to-antibody ratio (DAR) by UV/Vis spectroscopy  -- Yan Chen; title: Drug-to-antibody ratio (DAR) and drug load distribution by hydrophobic interaction chromatography and reversed phase high-performance liquid chromatography  -- Jun Ouyang; title: Drug-to-antibody ratio (DAR) and drug load distribution  by LC-ESI-MS  -- Louisette Basa; title: Determination of charge heterogeneity and level of unconjugated antibody by imaged cIEF  -- Joyce Lin and Alexandru C. Lazar; title: Risk-based scientific approach for determination  of extractables/leachables from biomanufacturing  of antibody-drug conjugates (ADCs) -- Weibing Ding.