Exon skipping
Subtitle: methods and protocols
By: edited by Annemieke Aartsma-Rus
Series: Methods in molecular biology -- 867; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- 867.; Springer protocols
Publisher: Humana Press
Published: 2012
Place of publication: New York
Language: eng
Number of pages: 440
ISBN-13: 9781617797668; 9781617797675
Subjects: Genetic Techniques; Genetics; Exons; Exons (Genetics); Laboratory Manuals; Laboratory manuals
Notes: Includes bibliographical references and index.
Table of contents: title: DNA diagnostics and exon skipping / Umasuthan Srirangalingam and Shern L. Chew; title: Bioinformatics and mutations leading to exon skipping / F.O. Desmet and C. Béroud; title: Minigenes to confirm exon skipping mutations / Lourdes R. Desviat, Belén Pérez, and Magdalena Ugarte; title: Analysis and interpretation of RNA splicing alterations in genes involved in genetic disorders / Maaike P.G. Vreeswijk and Heleen M. van der Klift; title: Exon skipping mutations in neurofibromatosis / Emanuele Buratti and Diana Baralle; title: Overview on applications of antisense-mediated exon skipping / Willeke M.C. van Roon-Mom and Annemieke Aartsma-Rus; title: Overview on DMD exon skipping / Annemieke Aartsma-Rus; title: Overview on AON design / Annemieke Aartsma-Rus; title: Optimizing RNA/ENA chimeric antisense oligonucleotides using in vitro splicing / Yasuhiro Takeshima, Mariko Yagi, and Masafumi Matsuo; title: Optimizing antisense oligonucleotides using phophorodiamidate morpholino oligomers / Linda J. Popplewell, Alberto Malerba, and George Dickson; title: Optimizing splice-switching oligomer sequences using 2'-O-methyl phosphorothioate chemistry / Carl Adkin, Sue Fletcher, and Steve D. Wilton; title: Exon skipping quantification by real-time PCR / Alessandra Ferlini and Paola Rimessi; title: Antisense-mediated exon skipping to shift alternative splicing to treat cancer / Jing Wan; title: Antisense-mediated exon skipping to generate soluble receptors / A. Seda Yilmaz-Elis and J. Sjef Verbeek; title: Antisense-mediated exon skipping to reframe transcripts / Sandrina Turczynski ... [et al.]; title: U1 snRNA as an effective vector for stable expression of antisense molecules and for the inhibition of the splicing reaction / Julie Martone, Fernanda Gabriella De Angelis, and Irene Bozzoni; title: Engineering U7snRNA gene to reframe transcripts / Aurélie Goyenvalle; title: Dynamic fluorescent and luminescent reporters for cell-based splicing screens / Claude C. Warzecha, Ruben Hovhannisyan, and Russ P. Carstens; title: Antisense-mediated exon-skipping to induce gene knockdown / Petra Disterer and Bernard Khoo; title: Antisense-mediated exon inclusion / Yimin Hua and Adrian R. Krainer; title: Antisense genes to induce exon inclusion / Rachel Nlend Nlend and Daniel Schümperli; title: Using mini-genes to identify factors that modulate alternative splicing / Robert Morse, Adrian G. Todd, and Philip J. Young; title: Overview of alternative oligonucleotide chemistries for exon skipping / Amer F. Saleh, Andrey A. Arzumanov, and Michael J. Gait; title: Identification of peptides for tissue-specific delivery / Hans Heemskerk; title: Systemic delivery of antisense oligomer in animal models and its implications for treating DMD / Qi Long Lu and Bo Wu; title: Cell-penetrating peptides enhance systemic delivery of antisense morpholino oligomers / Hong M. Moulton; title: Optimizing tissue-specific antisense oligonucleotide-peptide conjugates / Corinne A. Betts ... [et al.]