Studying and Improving Lambda Red Recombination for Genome Engineering in Escherichia coli
Contributors: Church, George M.; Kahne, Daniel E.; Silver, Pamela A.; Ausubel, Frederick N.
Published: 2013
Language: eng
Description: The phage-derived Lambda Red recombination system utilizes exogenous DNA in order to generate precise insertion, deletion, and point mutations in Escherichia coli and other bacteria. Due to its convenience, it is a frequently-used tool in genetics and molecular biology, as well as in larger-scale genome engineering projects. However, limited recombination frequency constrains the usefulness of Lambda Red for several important applications. In this work, I utilize a mechanism-guided approach in order to improve the power and utility of Lambda Red recombination.
Notes: Keywords: Lambda, Nuclease, Primase, Recombination, Recombineering, Red.

Thesis Ph.D. Harvard University 2013