Microglia
Subtitle: methods and protocols
By: edited by Bertrand Joseph, Department of Oncology-Pathology, Karolinska Institutet, Cancer Centrum Karolinska, Stockholm, Sweden, Jose Luis Venero, Departamento de Bioquimica y Biología, Instituto de Biomedicina de Sevilla, Hospital Universitario, Sevilla, Spain
Contributors: Springer Science+Business Media
Series: Methods in molceular biology -- 1041; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 1041.; Springer protocols (Series)
Publisher: Humana Press; Springer
Published: 2013
Place of publication: New York
Language: eng
Number of pages: 350
ISBN-13: 9781627035194; 9781627035200
Copyright date: 2013
Subjects: Microglia; Laboratory Manuals; Central Nervous System; Cytology
Notes: Includes bibliographical references and index.
Table of contents: title: Part I; title: Overview of microglia biology --; title: Brief overview of multitalented microglia  -- Bertrand Joseph and José Luis Venero -- Part II; title: Isolation and culture of microglia --; title: Cell culturing of human and murine microglia cell lines  -- Johanna Rodhe; title: Microglia isolation from adult mouse brain  -- Jae-Kyung Lee and Malú G. Tansey; title: Preparation of primary microglia cultures from postnatal mouse and rat brains  -- Tomas Deierborg; title: Isolation of murine postnatal brain microglia for phenotypic characterization using magnetic cell separation technology  -- Ashley S. Harms and Malú G. Tansey; title: Isolation and culture of adult human microglia within mixed glial cultures for functional experimentation and high-content analysis  -- Amy M. Smith ... [et al.] -- Part III; title: Depletion and transduction of microglia --; title: Depletion of microglia from primary cellular cultures  -- Lorena Pont-Lezica, Sabrina Colasse, and Alain Bessis; title: Lentiviral transduction of cultured microglia  -- Takahiro Masuda ... [et al.] -- Part IV; title: Analysis of microglial cytokine production --; title: Microglial activation : measurement of cytokines by flow cytometry  -- Deepak Kumar Kaushik and Anirban Basu; title: In situ hybridization of cytokine mRNA using alkaline phosphatase-labelled oligodeoxynucleotide probes  -- Bettina Clausen, Christina Fenger, and Bente Finsen; title: Use of meso-scale discovery to examine cytokine content in microglia cell supernatant  -- Miguel A. Burguillos -- Part V; title: Analysis of microglia activation --; title: Analysis of microglial production of reactive oxygen and nitrogen species  -- Urte Neniskyte and Guy C. Brown; title: Quantification of active caspase-3 and active caspase-8 in microglia cells  -- Edel Kavanagh; title: Quantification of microglial phagocytosis by a flow cytometer-based assay  -- Refik Pul, Kandiyil Prajeeth Chittappen, and Martin Stangel; title: Quantification of microglial proliferation and apoptosis by flow cytometry  -- Alicia A. Babcock, Martin Wirenfeldt, and Bente Finsen; title: Fluorescence imaging of intracellular Ca2+, Na+, and H+ in cultured microglia  -- Tom Schilling and Claudia Eder; title: Patch clamp protocols to study ion channel activity in microglia  -- Tom Schilling and Claudia Eder -- Part VI; title: Analysis of microglia polarization --; title: Studying M1 and M2 states in adult microglia  -- Sadanand M. Gaikwad and Michael T. Heneka; title: Isolating, culturing, and polarizing primary human adult and fetal microglia  -- Bryce A. Durafourt ... [et al.] -- Part VII; title: Co-culture systems to analysis microglia interactions with other cell types --; title: Understanding microglia-neuron cross talk : relevance of the microglia-neuron cocultures  -- Fernando G. Correa, Miriam Hernangómez, and Carmen Guaza; title: Preparation of rodent primary cultures for neuron-glia, mixed glia, enriched microglia, and reconstituted cultures with microglia  -- Shih-Heng Chen, Esteban A. Oyarzabal, and Jau-Shyong Hong -- Part VIII; title: Analysis of microglia functions in vivo --; title: Microglia detection by enzymatic histochemistry  -- Beatriz Almolda, Berta González, and Bernardo Castellano; title: Tomato lectin histochemistry for microglial visualization  -- Nàdia Villacampa ... [et al.]; title: Immunohistochemical detection of microglia  -- Rocío M. de Pablos, Ana M. Espinosa-Oliva, and Antonio J. Herrera; title: Intrathecal Infusion of Microglia Cells  -- Takahiro Masuda ... [et al.]; title: Intracranial injection of LPS in rat as animal model of neuroinflammation  -- Ana M. Espinosa-Oliva, Rocío M. de Pablos, and Antonio J. Herrera; title: Analyses of microglia effector function using CX3CR1-GFP knock-in mice  -- Jenny A. Garcia, Sandra M. Cardona, and Astrid E. Cardona; title: In vivo two-photon microscopy of microglia  -- Satoru Kondo and Shigeo Okabe; title: Use of confocal microscopy in the study of microglia  in a brain metastasis model -- Manuel Sarmiento.