Human monocyte response to oxidized ultra high molecular weight polyethylene sub-micron sized particles in vitro.
Published: 2005
Language: eng
Number of pages: 98
Description: Inflammatory tissue and periprosthetic osteolysis surrounding failed implants is attributed to the presence of UHMWPE wear debris particles and macrophages. The purpose of this thesis was to observe the response of human peripheral blood monocytes to the presence of oxidation on submicron sized oxidized UHMWPE particles. Oxidation was induced by gamma-irradiation and thermal treatment; FT-IR was used to characterize oxidation levels. Cell viability of 24 hour monocyte cultures was not affected by the presence of either virgin or oxidized particles (all containing 0.5 ppm of cobalt). At 24 hours, cytokine assays showed a significant increase in TNF-alpha, IL-1beta and IL-6 in the presence of submicron virgin UHMWPE. Some donors secreted less TNF-alpha and IL-1beta with oxidized particles. In the presence of oxidized particulate some donors were found to increase secretion of IL-6 and, in 7 day monocyte cultures, to have increased intracellular TRAP activity. Virgin UHMWPE did not increase TRAP activity levels in all donors. Since the response to the type and presence of oxidized particulate is dependent on the factor measured and donor, respectively, the findings stress the importance of studying PE surface chemistry using multiple variables (such as those use in this thesis) and multiple donors in future investigations on cellular response to wear debris particles.
Notes: Source: Masters Abstracts International, Volume: 44-02, page: 0958.

Thesis (M.A.Sc.)--University of Toronto, 2005.

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