Improving Zinc Finger Nucleases - Strategies for Increasing Gene Editing Activities and Evaluating Off-Target Effects
Contributors: Joung, J. Keith; Mulligan, Richard C.; Bulyk, Martha L.; Gill, Grace B.
Published: 2012
Language: eng
Description: Zinc finger nucleases (ZFNs) induce double-strand DNA breaks at specific recognition sites. ZFNs can dramatically increase the efficiency of incorporating desired insertions, deletions, or substitutions in living cells. These tools have revolutionized the field of genome engineering in several model organisms and cell types including zebrafish, rats, and human pluripotent stem cells. There have been numerous advances in ZFN engineering and characterization strategies, some of which are detailed in this work.
Notes: Keywords: cytotoxicity, genome engineering, modular assembly, nickase, ZFN, zinc finger.

Thesis Ph.D. Harvard University 2012