Lipases.
By: edited by Byron Rubin, Edward A. Dennis.
Contributors: Rubin, Byron.; Dennis, Edward A.
Series: Methods in enzymology -- v. 284.
Publisher: Academic Press
Published: 1997
Place of publication: San Diego
Language: eng
Number of pages: 408
Subjects: Lipase; Biotechnology; Lipase -- chemistry; Cloning, Molecular
Notes: Includes bibliographical references and indexes.
Table of contents: title: Sect.1. Primary and tertiary structure; title: Structure as a basis for understanding interfacial properties of lipases; title: Identification of conserved residues in family of esterase and lipase sequences; title: Identification of important motifs in protein sequences: program MULTIM and its applications tolipase-related sequences; title: Lipases and Ü/Ý hydrolase fold; title: Pancreatic lipases and their complexes with colipases and inhibitors: crystallization and crystal packing; title: Impact of structural information on understanding lipolytic function; title: Surface and electrostatics of cutinases; title: Sect.2. Isolation, cloning, expression, and engineering; title: Site-specific mutagenesis of human pancreatic lipase; title: Lipase eingineering: a window into strucutre-function relationships; title: Purification of carboxyl ester lipase (bile salt-stimulated lipase) from human milk and pancreas; title: Two novel lipases from thermophile Bacillus thermocatenulatus: screening, purificatin, cloning, overexpression, and properties; title: Vernonia lipase: a plant lipase with strong fatty acid slectivity; title: Hepatic lipase: high-level expression and subunit structure determination; title: Cloning, sequencing, and expression of Candida rugosa lipases; title: Influence of various signal peptides on secretion of mammalian acidic lipases in baculovirus-insect cell system; title: Large-scale purification and kinetic properties of recombinant hormone-sensitive lipase from baculovirus-insect cell systems; title: New pancreatic lipases: gene expression, protein secretion, and the newborn; title: Structure and function of engineered Pseudomonas mendocina lipase; title: Protein engineering of microbial lipases of industrial interest; title: Glycosylation of bile salt-dependent lipase (cholesterol esterase); title: Steroselectivity of lipase from Rhizopus oryzae toward triaclglycerols and analogs: computer-aided modeling and experimental validation.