Characterization of the novel mu opioid and D(1) dopamine receptor heterooligomer.
Published: 2006
Language: eng
Number of pages: 109
ISBN-13: 9780494161883
Description: Literature has suggested a possible association between the mu opioid and D1 dopamine receptors. Using BRET and a NLS-based assay, the mu opioid and D1 dopamine receptors were shown to specifically interact through the formation of a stable heterooligomer. Competition binding demonstrated no considerable changes in the binding affinities of either receptor following co-expression. However, single-point saturation binding displayed a significant increase in cell surface expression of the muOR upon co-expression with D1R, indicating novel regulation of receptor trafficking. Additionally, binding indicated a reduction in cell surface expression of the muOR upon co-expression with D5R, suggesting that these receptors may also heterooligomerize. A chimeric receptor approach was also utilized and suggests that select regions of the D1R are required for promoting muOR cell surface expression. Furthermore, immunocytochemistry demonstrated that these receptors co-localize in neurons of rat brain, supporting the possibility that these receptors may come into direct contact in vivo.
Notes: Source: Masters Abstracts International, Volume: 44-06, page: 2775.

Thesis (M.Sc.)--University of Toronto, 2006.

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