Practical biotransformations
Subtitle: a beginner's guide
By: Gideon Grogan.
Series: Postgraduate chemistry series
Publisher: Wiley
Published: 2009
Place of publication: Chichester, West Sussex
Language: eng
ISBN-13: 9781405193672; 9781405171250
Subjects: Biosynthesis; Enzymes -- Biotechnology; Microbial biotechnology; Organic compounds -- Synthesis
Notes: Includes bibliographical references and index.
Table of contents: title: Biotransformations, microbes and enzymes; title: Introduction; title: Biotransformations; title: Microorganisms; title: Organism nomenclature; title: Enzymes; title: Types of enzymatic reactions; title: Enzymatic cofactors; title: Some basic characteristics of enzyme catalysis; title: Types of biocatalyst biotransformations by whole cells or isolated enzymes; title: Conclusion; title: An overview of biocatalyst sources and web-based information; title: Introduction; title: Microbial culture collections; title: Obtaining organisms from other research groups; title: Selective enrichments; title: Metagenomics; title: Enzyme suppliers and biocatalyst development companies; title: Genome mining for biocatalysts; title: Obtaining amino acid and gene sequence information on biocatalysts; title: Obtaining DNA templates for cloning; title: Custom gene synthesis; title: Other interesting web resources for biocatalysis; title: Conclusion; title: Setting up a laboratory for biotransformations; title: Introduction; title: Microbiological containment; title: On containment issues and genetically-modified organisms; title: Equipment for handling microorganisms; title: Techniques and terms in microbiology sterility, asepsis and aseptic technique; title: Disposal of viable microbial waste and disinfection of reusable equipment; title: Equipment for enzymology and molecular biology; title: General reagents and chemicals in a biotransformations laboratory; title: Conclusion; title: A beginner's guide to preparative whole-cell microbial biotransformations; title: Introduction; title: Storage, maintenance and growth of microorganisms; title: General microbiological methods; title: Examples of whole-cell biotransformations using bacteria; title: Biotransformation by filamentous fungi and yeasts; title: Whole-cell biotransformations by recombinant strains of E. coli; title: Conclusion; title: A beginner's guide to biotransformations by commercially available isolated enzymes; title: Introduction; title: Lipases; title: Hydrolytic reactions using lipases; title: Using lipases for acylation reactions; title: Other hydrolases; title: Commercially available coenzyme-dependent enzymes; title: Carbon-carbon bond forming reactions; title: Conclusion; title: A beginner's guide to the isolation and analysis and use of home-grown enzyme biocatalysts; title: Introduction; title: Cell growth and harvesting; title: Cell disruption; title: A typical procedure for making a cell extract from a recombinant strain of E. coli; title: Purification of enzymes - a brief guide; title: Techniques for protein purification; title: Isolation of recombinant enzymes using histidine tags; title: Estimation of protein.