Protein engineering
By: edited by Dan E. Robertson, Joseph P. Noel.
Contributors: Robertson, Dan E.; Noel, Joseph P.
Series: Methods in enzymology -- v. 388.
Publisher: Elsevier Academic Press
Published: 2004
Place of publication: Amsterdam; Boston
Language: eng
Number of pages: 413
Subjects: Protein engineering.; Proteins -- Synthesis.; Genetic engineering.; Proteins -- Structure-activity relationships.; Protein Conformation.; Proteins -- genetics.
Notes: Includes bibliographical references and indexes.
Table of contents: title: Section I. Methodology; title: Gene site saturation mutagenesis : a comprehensive mutagenesis approach; title: Single-stranded DNA family shuffling; title: Module shuffling; title: SCHEMA-guided protein recombination; title: Staggered extension process in vitro DNA recombination; title: Using incremental truncation to create libraries of hybrid enzymes; title: Random multirecombinant polymerase chain reaction; title: Gene library synthesis by structure-based combinatorial protein engineering; title: New enzymes from combinatorial library modules; title: Construction of protein fragment complementation libraries using incremental truncation; title: Section II. Applications: optimization and screening; title: GigaMatrix: a novel ultrahigh throughput protein optimization and discovery platform; title: High throughput microplate screens for directed protein evolution; title: Periplasmic expression as a basis for whole cell kinetic screening of unnatural enzyme reactivities; title: Engineering the thermotolerance and pH optimum of family 11 xylanases by site-directed mutagenesis; title: Screening for oxidative resistance; title: Consensus-based engineering of protein stability: from intrabodies to thermostable enzymes; title: Improving the functional expression of N-Carbamoylase by directed evolution using the green fluorescent protein fusion reporter system; title: Section III. Applications: directed evolution of enzymatic function; title: Directed evolution of lipases and esterases; title: Protein engineering of the cytochrome P450 monooxygenase from bacillus megaterium; title: Directed evolution of aldolases; title: Changing the enantioselectivity of enzymes by directed evolution; title: Control of stereoselectivity in phosphotriesterase; title: Section IV. Applications: evolution of biosynthetic pathways; title: Manipulation and analysis of polyketide synthases; title: Reactions catalyzed by mature and recombinant nonribosomal peptide synthetases; title: Engineering carotenoid biosynthetic pathways; title: Section V. Devices, antibodies, and vaccines; title: Directed evolution of bacteriorhodopsin for device applications; title: Engineering antibody affinity by yeast surface display; title: A conditionally replicating virus as a novel approach toward an HIV vaccine.