Two-dimensional electrophoresis protocols
By: edited by David Sheehan, Raymond Tyther
Series: Methods in molecular biology -- v. 519; Springer protocols; Methods in molecular biology -- v. 519.; Springer protocols
Publisher: Humana
Published: 2009
Place of publication: New York
Language: eng
Number of pages: 545
ISBN-13: 9781588299376; 9781597452816
Subjects: Two-dimensional electrophoresis; Methode; Electrophoresis, Gel, Two-Dimensional; Methods; Zweidimensionale Elektrophorese
Notes: Includes bibliographical references and index.
Table of contents: title: Two-dimensional electrophoresis : an overview / Richard Smith; title: Solubilization of proteins in 2DE : an outline / Thierry Rabilloud; title: Selection of pH ranges in 2DE / Mireille Starita-Geribaldi; title: Difficult proteins / Ben Herbert and Elizabeth Harry; title: Organelle proteomics / Matthias Plöscher ... [et al.]; title: Applications of chemical tagging approaches in combination with 2DE and mass spectrometry / Alexander Leitner and Wolfgang Lindner; title: Immunoblotting 2DE membranes / Brian McDonagh; title: Troubleshooting image analysis in 2DE / Bettina Levänen and Åsa M. Wheelock; title: Analysis of bacterial proteins by 2DE / Philip Cash and Evelyn Argo; title: Proteomic analysis of Caenorhabditis elegans / Pan-Young Jeong ...[et al.]; title: Protein extraction for 2DE / Claus Zabel and Joachim Klose; title: Analysis of proteins from marine molluscs / Suze Chora, Maria João Bebianno, and Michèle Roméo; title: Preparation and analysis of plastid proteomes by 2DE / Anne von Zychlinski and Wilhelm Gruissem; title: High resolution 2DE / Katrin Marcus ...[et al.]; title: Blue native-gel electrophoresis proteomics / Kelly Andringa, Adrienne King, and Shannon Bailey; title: 2DE for proteome analysis of human metaphase chromosomes / Kiichi Fukui and Susumu Uchiyama; title: Microsomal proteomics / Diana M. Wong and Khosrow Adeli; title: Prefractionation using microscale solution IEF / Won-A Joo and David Speicher; title: Diagonal electrophoresis for detection of protein disulphide bridges / Brian McDonagh; title: High-resolution large-gel 2DE / Claus Zabel and Joachim Klose; title: Silver staining of proteins in 2DE gels / Cécile Lelong ...[et al.]; title: Detection of 4-hydroxy-2-nonenal- and 3-nitrotyrosine-modified proteins using a proteomics approach / Rukhsana Sultana, Tanea Reed, and D. Allan Butterfield; title: Proteomic detection of oxidized and reduced thiol proteins in cultured cells / Sarah L. Cuddihy ...[et al.]; title: Detection of ubiquitination in 2DE / Brian McDonagh; title: Phosphoroproteome analysis by in-gel isoelectric focusing and tandem mass spectrometry / Sarka Beranova-Georgianni, Dominic M. Desiderio, and Francesco Giorgianni; title: Detection of protein glutathionylation / Elisabetta Gianazza, Ivano Eberini, and Pietro Ghezzi; title: Activity-based protein profiling of protein tyrosine phosphatases / Chad Walls, Bo Zhou, and Zhong-Yin Zhang; title: Active protease mapping in 2DE gels / Zhenjun Zhao and Pamela J. Russell; title: Two-dimensional difference gel electrophoresis / Gert Van den Bergh; title: Protein expression profiling / Brian P. Bradley, Bose Kalampanayil, and Michael C. O'Neill; title: C-terminal sequence analysis of 2DE-separated proteins / Bart Samyn, Kjell Sergeant, and Jozef Van Beeumen; title: Shotgun protein analysis by liquid chromatography-tandem mass spectrometry / Kazuishi Kubota, Toshiyuki Kosaka, and Kimihisa Ichikawa; title: De novo sequence analysis of N-terminal sulfonated peptides after in-gel guanidination / Kjell Sergeant, Jozef Van Beeumen, and Bart Samyn; title: Tryptic digestion of in-gel proteins for mass spectrometry analysis / Mai-Loan Huynh, Pamela Russell, and Bradley Walsh; title: Database interrogation algorithms for identification of proteins in proteomic separations / Patricia M. Palagi, Frédérique Lisacek, and Ron D. Appel; title: Creating 2DE databases for the world wide web / Christine Hoogland, Khaled Mostaguir, and Ron D. Appel.