Test No. 412 : Subacute Inhalation Toxicity
Subtitle: 28-Day Study
By: Organisation for Economic Co-operation and Development
Series: OECD Guidelines for the Testing of Chemicals, Section 4
Publisher: OECD Publishing
Published: 2009
Place of publication: Paris
Language: eng
Format: [electronic resource] /
Number of pages: 14
ISBN-13: 9789264070783
Subjects: Ecology; Euthenics; Nature and nurture; Adaptation (Biology)
Description: This revised Test Guideline 412 (TG 412) has been designed to fully characterize test article toxicityby the inhalation route following repeated exposure for a limited period of time (28 days), and to provide data for quantitative inhalation risk assessments. Groups of at least 5 male and 5 female rodents are exposed 6 hours per day for 28 days to a) the test article at three or more concentration levels, b) filtered air (negative control), and/or c) the vehicle (vehicle control). Animals are generally exposed 5 days per week but exposure for 7 days per week is also allowed. Males and females arealways tested, but they may be exposed at different concentration levels if it is known that one sex ismore susceptible to a given test article. This guideline allows the study director the flexibility toinclude satellite (reversibility) groups, bronchoalveolar lavage (BAL), neurologic tests, and additionalclinical pathology and histopathological evaluations in order to better characterize the toxicity of a testarticle.