Electrokinetically-driven micofluidic devices for DNA extraction and analysis.
Published: 2006
Language: eng
Number of pages: 121
ISBN-13: 9780494161708
Description: This thesis focuses on electrokinetically-driven microfluidic devices for DNA analysis. These are designed, optimized via numerical simulation, and experimentally tested. Processes considered include solid phase extraction (SPE) of DNA, and DNA hybridization for the detection of single-nucleotide polymorphism (SNP) genetic mutations. Also included is an experimental analysis of the influence of temperature on the zeta potential parameter (and microchannel flow velocity). The SPE device was successful with input samples of pre-purified lambda-DNA and whole HeLa cancer cells, with a 68% maximum efficiency. The DNA hybridization device was capable of detecting the SNP associated with the childhood disease, Spinal Muscular Atrophy, using both pre-purified and polymerase chain reaction (PCR)-amplified DNA. Zeta potential experiments concluded that many buffers commonly-used in microfluidic applications experience a strong dependence on temperature, contrary to common treatment of this variable. Failure to compensate for this can result in underestimation of in-channel flow velocity as high as 30%.
Notes: Source: Masters Abstracts International, Volume: 44-06, page: 2983.

Thesis (M.A.Sc.)--University of Toronto, 2006.

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