In vitro neurotoxicology
Subtitle: methods and protocols
By: edited by Lucio G. Costa, Gennaro Giordano, Marina Guizzetti
Series: Methods in molecular biology -- 758; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 758.; Springer protocols
Publisher: Humana/Springer
Published: 2011
Place of publication: New York
Language: eng
Number of pages: 444
ISBN-13: 9781617791697; 9781617791703
Subjects: Toxicity testing; Neurotoxins; Laboratory Manuals; Neurotoxicology; Toxicity Tests; In vitro; Toxicity; Laboratory manuals; Methods
Notes: Includes bibliographical references and index.
Table of contents: title: In vitro neurotoxicology : an introduction  -- Lucio G. Costa, Gennaro Giordano, and Marina Guizzetti; title: Primary neurons in culture and neuronal cell lines for in vitro neurotoxicological studies  -- Gennaro Giordano and Lucio G. Costa; title: Determination of metal interactions with the chaperone Hspa5 in human astrocytoma cells and rat astrocyte primary cultures  -- Evelyn Tiffany-Castiglioni ... [et al.]; title: Microglia cell culture: a primer for the novice  -- Anke Witting and Thomas Möller; title: Neural stem cells for developmental neurotoxicity studies  -- Roshan Tofighi ... [et al.]; title: Preparation, maintenance, and use of serum-free aggregating brain cell cultures  -- Paul Honegger ... [et al.]; title: Neurospheres as a model for developmental neurotoxicity testing  -- Ellen Fritsche, Kathrin Gassmann, and Timm Schreiber; title: Acute hippocampal slice preparation and hippocampal slice Cultures  -- Pamela J. Lein, Christopher D. Barnhart, and Isaac N. Pessah; title: Glia-neuron sandwich cocultures : an in vitro approach to evaluate cell-to-cell communication in neuroinflammation and neurotoxicity  -- Mariaserena Boraso and Barbara Viviani; title: In vitro models to study the blood brain barrier  -- Hilary Vernon, Katherine Clark, and Joseph P. Bressler; title: Measurements of cell death in neuronal and glial cells  -- Gennaro Giordano ... [et al.]; title: Measurements of neuronal apoptosis  -- Gennaro Giordano and Lucio G. Costa; title: Measurement of isoprostanes as markers of oxidative stress  -- Dejan Milatovic, Thomas J. Montine, and Michael Aschner; title: Assessment of glutathione homeostasis  -- Gennaro Giordano, Collin C. White, and Lucio G. Costa; title: Assessing neuronal bioenergetic status  -- Stephanie L.H. Zeiger, Jeannette N. Stankowski, and BethAnn McLaughlin; title: Fluorescent assessment of intracellular calcium in individual cells  -- David P. Cox, Collin C. White, and Terrance J. Kavanagh; title: Homeostatic regulation of glutamate neurotransmission in primary neuronal cultures  -- Victor Briz and Cristina Suñol; title: Detection of nitric oxide formation in primary neural cells and tissues  -- Ronald B. Tjalkens, David L. Carbone, and Guoyao Wu; title: JNK3-mediated apoptotic cell death in primary dopaminergic neurons  -- Won-Seok Choi, Heather M. Klintworth, and Zhengui Xia; title: Measurement of proteasomal dysfunction in cell models of dopaminergic degeneration  -- Faneng Sun, Anumantha G. Kanthasamy, and Arthi Kanthasamy; title: Cell signaling and neurotoxicity : protein kinase C in vitro and in vivo  -- Prasada Rao S. Kodavanti; title: Cell signaling and neurotoxicity : ³H-arachidonic acid release (phospholipase A₂) in cerebellar granule neurons  -- Prasada Rao S. Kodavanti; title: Quantitative assessment of neurite outgrowth in PC12 cells  -- Joshua A. Harrill and William R. Mundy; title: Measurements of astrocyte proliferation  -- Marina Guizzetti, Terrance J. Kavanagh, and Lucio G. Costa; title: Quantification of synaptic structure formation in cocultures of astrocytes and hippocampal neurons  -- Pamela J. Roqué ... [et al.]; title: Volume measurements in cultured primary astrocytes  -- Michael Aschner; title: Assessment of cholesterol homeostasis in astrocytes and neurons  -- Jing Chen, Lucio G. Costa, and Marina Guizzetti; title: Dose-response or dose-effect curves in in vitro experiments and their use to study combined effects of neurotoxicants -- Matteo Goldoni and Sara Tagliaferri.