Breast cancer research protocols
By: edited by Susan A. Brooks, Adrian Harris.
Contributors: Brooks, Susan A.; Harris, Adrian, 1950-
Series: Methods in molecular medicine -- 120
Publisher: Humana Press
Published: 2006
Place of publication: Totowa, N.J
Language: eng
Number of pages: 517
ISBN-13: 9781588291912; 9781592599691
Subjects: Breast -- Cancer -- Laboratory manuals; Breast Neoplasms -- genetics -- Laboratory Manuals; Breast Neoplasms -- pathology -- Laboratory Manuals; Genetic Techniques
Genres: Laboratory manuals.; Laboratory Manuals.
Notes: Includes bibliographical references and index.
Table of contents: title: Breast tissue banking: collection, handling, storage, and release of tissue for breast cancer research / Linda Snell and Peter H. Watson; title: Frozen biopsy collection and storage / Russell D. Leek; title: Serial biopsies/FNAs and their assessment / Irene Boeddinghaus and Stephen R.D. Johnson; title: Breast tissue microarrays / Robert J. Springall and Cheryl E. Gillett; title: Preparation of tumor homogenates for subsequent preparation of cytosols, membrane fractions, RNA, and DNA / Naomi Robertson and Russell Leek; title: Isolation of RNA from tumor samples: single-step guanidinium acid/phenol method / Naomi Robertson and Russell Leek; title: Isolation of DNA from tumor samples / Naomi Robertson and Russell Leek; title: Laser-assisted microdissection and isolation of DNA and RNA / Ulrich Lehmann and Hans Kreipe; title: Traditional and established indicators of prognosis and treatment success / Derek E. Roskell and Ian D. Buley; title: Sentinel lymph node biopsy in breast cancer / Celia Chao and Kelly M. McMasters; title: Pathological evaluation of axillary sentinel lymph nodes in breast cancer / Michael Z. Gilcrease and Aysegul Sahin; title: Measurement of estrogen receptor status by immunocytochemistry in paraffin wax sections / Bharat Jasani ... [et al.]; title: Markers of apoptosis / Falzul H. Sarkar and Yiwei Li; title: Quantitative angiogenesis in breast cancer / Stephen B. Fox; title: Immunohistochemistry / Cheryl E. Gillett; title: Detection of aberrant glycosylation in breast cancer using lectin histochemistry / Tracey M. Carter and Susan A. Brooks; title: SDS-PAGE and western blotting to detect proteins and glycoproteins of interest in breast cancer research / Chloe Osborne and Susan A. Brooks; title: Breast cancer proteomics using two-dimensional electrophoresis / Miriam V. Dwek and Sarah L. Rawlings; title: Procedures for the quantitative protein determination of urokinase and its inhibitor PAI-1 in human breast cancer tissue extracts using ELISA / Manfred Schmitt ... [et al.]; title: Fluorescence in situ hybridization and comparative genomic hybridization / Patricia Gorman and Rebecca Roylance; title: Fluorescence in situ hybridization assessment of c-myc amplification in breast cancer tissues / Jan K. Blancato, Mary Steele Williams, and Robert B. Dickson; title: Detection of HER2 gene amplification by FISH in breast cancer / John M.S. Bartlett, and Amanda Forsyth; title: In situ hybridization combined with immunohistochemistry to localize gene expression / Rosemary Jeffery, Toby Hunt, and Richard Poulsom; title: Quantitation of RNA by ribonuclease protection assay / John W. Moore; title: Identification of steroid hormone-regulated genes in breast cancer / Bruce R. Westley and Felicity E.B. May; title: Sequencing of the tumor suppressor gene TP 53 / Barbro Linderholm, Torbjorn Norberg, and Jonas Bergh; title: Expression profiling using cDNA microarrays / Chris Jones ... [et al.]; title: Gene expression analysis using filter cDNA microarrays / Peter Simpson ... [et al.]; title: Methods to analyze the effects of the urokinase system on cancer cell adhesion, proliferation, migration, and signal transduction events / Ute Reuning ... [et al.]; title: Phosphospecific antibodies as a tool to study in vivo regulation of BRCA1 after DNA damage / Kum Kum Khanna, Magtouf Gatei, and Gordon Tribbick; title: Models of hormone resistance in vitro and in vivo / Jennifer MacGregor Schafer and V. Craig Jordan; title: Generation of genetically modified embryonic stem cells for the development of knockout mouse animal model systems / Stephen D. Robinson, Stephen Wilson, and Kairbaan M. Hodivala-Dilke; title: In vivo xenograft models of breast cancer metastatsis / Ursula Valentiner, Susan A. Brooks, and Udo Schumacher; title: Neoadjuvant endocrine therapy models / Juliette Murray, William R. Miller, and J. Michael Dixon; title: Primary mouse endothelial cell culture for assays of angiogenesis / Louise E. Reynolds and Kairbaan M. Hodivala-Dilke.