Vascular proteomics
Subtitle: methods and protocols
By: edited by Fernando Vivanco
Series: Methods in molecular biology -- 1000; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- 1000.; Springer protocols (Series)
Publisher: Humana Press; Springer
Published: 2013
Place of publication: New York
Language: eng
Number of pages: 230
ISBN-13: 9781627034043; 9781627034050
Subjects: Cardiovascular system; Laboratory manuals; Proteomics; Blood Vessels; Laboratory Manuals; Metabolism; Methods; Biological Markers; Analysis; Cardiovascular Diseases; Blutgefäss; Kardiovaskuläre Krankheit; Proteomanalyse; Biomarker; Stoffwechsel
Description: Proteomics is a rapidly expanding investigation platform in cardiovascular medicine. Driven by major improvements in mass spectrometry (MS) instrumentation and data analysis, the proteomics field has flourished in recent years particularly in the study of complex diseases. These recent advances are characterized by the development of quantitative MS-based methods that promoted the field from primarily identifying proteins to also providing measurements of relative changes in protein levels between different cell states. This progress is reflected in the application of proteomic techniques to vascular pathology. Vascular Proteomics: Methods and Protocols provides up-to-date methods and protocols for the analysis of arteries, cells, lipoproteins, body fluids, and metabolites, with a particular focus on MS-based methods of protein and peptide quantification. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Vascular Proteomics: Methods and Protocols is a representative selection of methods that can be a useful resource for experienced proteomics practitioners as well as newcomers interested in becoming acquainted with the practice of proteomic techniques for cardiovascular research.--
Notes: Includes bibliographical references and index.
Table of contents: title: Vascular proteomics / Maria G. Barderas, Fernando Vivanco, and Gloria Alvarez-Llamas; title: Laser microdissection and saturation labeling DIGE method for the analysis of human arteries / Fernando de la Cuesta ... [et al.]; title: Use of TOF-SIMS in vascular biology / Sebastián Mas, Raúl Pérez, and Jesús Egido; title: Proteomic analysis of vascular smooth muscle cells with S- and R-enantiomers of atenolol by iTRAQ and LC-MS/MS / Jianhua Zhang ... [et al.]; title: Phosphoproteomic analysis of aortic endothelial cells activated by oxidized phospholipids / Alejandro Zimman, Judith A. Berliner, and Thomas G. Graeber; title: Characterization of membrane and cytosolic proteins of erythrocytes / Gloria Alvarez-Llamas ... [et al.]; title: Characterization and analysis of human arterial tissue secretome by 2-DE and nLC-MS/MS / Fernando de la Cuesta ... [et al.]; title: Identification of novel biomarkers of abdominal aortic aneurysms by 2D-DIGE and MALDI-MS from AAA-thrombus-conditioned media / Roxana Martinez-Pinna ... [et al.]; title: Metabolites secreted by human atherothrombotic aneurysm / Michal Ciborowski and Coral Barbas; title: Quantitative analysis of apolipoproteins in human HDL by top-down differential mass spectrometry / Matthew T. Mazur and Helene L. Cardasis; title: Quantitative proteomics analysis of high-density lipoproteins by stable ¹⁸O-isotope labeling / Elena Burillo, Jesus Vazquez, and Inmaculada Jorge; title: Unraveling biomarkers of abdominal aortic aneurisms by iTRAQ analysis of depleted plasma / Enrique Calvo ... [et al.]; title: Absolute quantitation of proteins in human blood by multiplexed multiple reaction monitoring mass spectrometry / Andrew J. Percy ... [et al.]; title: Multiple reaction monitoring (MRM) of plasma proteins in cardiovascular proteomics / Verónica M. Dardé, Maria G. Barderas, and Fernando Vivanco; title: Proteomic analysis of plasma of patients with left ventricular remodeling after myocardial infarction : usefulness of SELDI-TOF / Florence Pinet; title: Proteomic analysis of urinary exosomes in cardiovascular and associated kidney diseases by two-dimensional electrophoresis and LC-MS/MS / Irene Zubiri, Fernando Vivanco, and Gloria Alvarez-Llamas.