Mucins
Subtitle: methods and protocols
By: edited by Michael A. McGuckin, David J. Thornton
Series: Methods in molecular biology -- 842; Methods in molecular biology (Clifton, N.J.) -- v. 842
Publisher: Humana Press
Published: 2012
Place of publication: New York
Language: eng
Number of pages: 329
ISBN-13: 9781617795121; 9781617795138
Subjects: Mucins; Laboratory manuals
Notes: Includes bibliographical references and index.
Table of contents: title: Mucin methods : genes encoding mucins and their genetic variation with a focus on gel-forming mucins  -- Karine Rousseau and Dallas M. Swallow; title: Gel-forming and cell-associated mucins : preparation for structural and functional studies  -- Julia R. Davies, Claes Wickstrom, and David J. Thornton; title: Detecting, visualising, and quantifying mucins  -- Ceri A. Harrop, David J. Thornton, and Michael A. McGuckin; title: Mass spectrometric analysis of mucin core proteins  -- Mehmet Kesimer and John K. Sheehan; title: O-glycoprotein biosynthesis : site localization by edman degradation and site prediction based on random peptide substrates  -- Thomas A. Gerken; title: Analysis of assembly of secreted mucins  -- Malin E.V. Johansson and Gunnar C. Hansson; title: MUC1 membrane trafficking : protocols for assessing biosynthetic delivery, endocytosis, recycling, and release through exosomes  -- Franz-Georg Hanisch ... [et al.]; title: Glycomic work-flow for analysis of mucin o-linked Oligosaccharides  -- Catherine A. Hayes ... [et al.]; title: O-glycomics : profiling and structural analysis of mucin-type o-linked glycans  -- Isabelle Breloy; title: O-glycoproteomics : site-specific o-glycoprotein analysis by CID/ETD electrospray ionization tandem mass spectrometry and top-down glycoprotein sequencing by in-source decay MALDI mass spectrometry  -- Franz-Georg Hanisch; title: Analysing mucin degradation  -- Stephen D. Carrington ... [et al. ]; title: Assessment of mucus thickness and production in situ  -- Lena Holm and Mia Phillipson; title: Preservation of mucus in histological sections, immunostaining of mucins in fixed tissue, and localization of bacteria with FISH  -- Malin E.V. Johansson and Gunnar C. Hansson; title: Ex vivo measurements of mucus secretion by colon explants  -- Jenny K. Gustafsson, Henrik Sjovall, and Gunnar C. Hansson; title: Establishment of respiratory air-liquid interface cultures  and their use in studying mucin production, secretion and function  -- David B. Hill and Brian Button; title: Studying mucin secretion from human bronchial epithelial cell primary cultures  -- Lubna H. Abdullah ... [et al.]; title: Assessment of intracellular mucin content in vivo  -- Lucia Piccotti, Burton F. Dickey, and Christopher M. Evans; title: Techniques for assessment of interactions of mucins with microbes and parasites in vitro and in vivo  -- Yong H. Sheng ... [et al.]; title: Assessing mucin expression and function in human ocular surface epithelia in vivo and in vitro -- Pablo Argueso and Ilene K. Gipson.