DNA damage detection in situ, ex vivo, and in vivo
Subtitle: methods and protocols
By: edited by Vladimir V. Didenko
Series: Methods in molecular biology -- v. 682; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 682.; Springer protocols
Publisher: Humana Press
Published: 2011
Place of publication: New York; London
Language: eng
Number of pages: 313
ISBN-13: 9781603274081
Subjects: DNA damage; DNA Damage; DNA; Analysis; Research; Methodology; Microbiological Techniques; Laboratory Manuals
Notes: Includes bibliographical references and index.

Also available online.
Table of contents: title: In situ detection of apoptosis by the TUNEL assay : an overview of techniques / Deryk T. Loo; title: Combination of TUNEL assay with immunohistochemistry for simultaneous detection of DNA fragmentation and oxidative cell damage / Alexander E. Kalyuzhny; title: EM-ISEL : a useful tool to visualize DNA damage at the ultrastructural level / Antonio Migheli; title: In situ labeling of DNA breaks and apoptosis by T7 DNA polymerase / Vladimir V. Didenko; title: In situ ligation : a decade and a half of experience / Peter J. Hornsby and Vladimir V. Didenko; title: In situ ligation simplified : using PCR fragments for detection of double-strand DNA breaks in tissue sections / Vladimir V. Didenko; title: 5'OH DNA breaks in apoptosis and their labeling by topoisomerase-based approach / Vladimir V. Didenko; title: Detection of DNA strand breaks in apoptotic cells by flow- and image-cytometry / Zbigniew Darzynkiewicz and Hong Zhao; title: Fluorochrome-labeled inhibitors of caspases : convenient in vitro and in vivo markers of apoptotic cells for cytometric analysis / Zbigniew Darzynkiewicz ... [et al.]; title: Combining fluorescent in situ hybridization with the comet assay for targeted examination of DNA damage and repair / Sergey Shaposhnikov, Preben D. Thomsen, and Andrew R. Collins; title: Simultaneous labeling of single- and double-strand DNA breaks by DNA breakage detection-FISH (DBD-FISH) / José Luis Fernández, Dioleyda Cajigal, and Jaime Gosálvez; title: Co-localization of DNA repair proteins with UV-induced DNA damage in locally irradiated cells / Jennifer Guerrero-Santoro, Arthur S. Levine, and Vesna Rapić-Otrin; title: Ultrasound imaging of apoptosis : spectroscopic detection of DNA-damage effects at high and low frequencies / Roxana M. Vlad, Michael C. Kolios, and Gregory J. Czarnota; title: Quantifying etheno-DNA adducts in human tissues, white blood cells, and urine by ultrasensitive ³²P-postlabeling and immunohistochemistry / Jagadeesan Nair ... [et al.]; title: ELISpot assay as a tool to study oxidative stress in peripheral blood mononuclear cells / Jodi Hagen, Jeffrey P. Houchins, and Alexander E. Kalyuzhny; title: Cytokinesis-block micronucleus cytome assay in lymphocytes / Philip Thomas and Michael Fenech; title: Buccal micronucleus cytome assay / Philip Thomas and Michael Fenech; title: [Gamma]-H2AX detection in peripheral blood lymphocytes, splenocytes, bone marrow, xenografts, and skin / Christophe E. Redon ... [et al.]; title: Immunologic detection of benzo(a)pyrene-DNA adducts / Regina M. Santella and Yu-Jing Zhang; title: Non-invasive assessment of oxidatively damaged DNA : liquid chromatography-tandem mass spectrometry analysis of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine / Vilas Mistry ... [et al.]; title: Assessing sperm DNA fragmentation with the sperm chromatin dispersion test / José Luis Fernández ... [et al.]