Cell cycle checkpoints
Subtitle: methods and protocols
By: edited by Willis X. Li
Series: Springer protocols; Methods in molecular biology -- 782; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 782.
Publisher: Humana Press/Springer
Published: 2011
Place of publication: New York
Language: eng
Number of pages: 307
ISBN-13: 9781617792724; 9781617792731
Subjects: Cell cycle; Laboratory manuals
Notes: Includes bibliographical references and index.
Table of contents: title: 1. Studying G2 DNA damage checkpoints using the fission yeast schizosaccharomyces pombe / Nicholas Willis and Nicholas Rhind; title: 2. Studying S-phase DNA damage checkpoints using the fission yeast schizosaccharomyces pombe / Nicholas Willis and Nicholas Rhind; title: 3. Methods for studying the G2 DNA damage checkpoint in mammalian cells / Claudia Tapia-Alveal and Matthew J. O'Connell; title: 4. Evaluating spindle assembly checkpoint competence in mouse oocytes using immunoblotting / Hayden Homer; title: 5. Analysis of changes in protein level and subcellular localization during cell cycle progression using the budding yeast saccharomyces cerevisiae / Xiaorong Wu, Lili Liu, and Mingxia Huang; title: 6. Studying cell cycle checkpoints using drosophila cultured cells / Katarzyna Siudeja, Jannie de Jong, and Ody C.M. Sibon; title: 7. In vivo live-analysis of cell cycle checkpoints in drosophila early embryos / Saeko Takada and Byeong J. Cha; title: 8. Using drosophila larval imaginal discs to study low-dose radiation-induced cell cycle arrest / Shian-Jang Yan and Willis X. Li; title: 9.Screening for radiation sensitizers of drosophila checkpoint mutants / Mara Gladstone and Tin Tin Su; title: 10. Study of cell cycle checkpoints using xenopus cell-free extracts / Seetha V. Srinivasan and Jean Gautier; title: 11. Analyzing DNA replication dynamics of genotoxin-treated cells using velocity sedimentation / Tovah A. Day, Chris Sproul, Marila Cordeiro-Stone, and Cyrus Vaziri; title: 12. Methods for studying checkpoint kinases-chk1 / Claudia Tapia-Alveal and Matthew J. O'Connell; title: 13. A human cell extract-based assay for the activation of ATM and ATR checkpoint kinases / Bunsyo Shiotani and Lee Zou; title: 14. Analyzing p53 regulated DNA damage checkpoints by flow cytometry / Lois Resnick-Silverman and James J. Manfredi; title: 15. Using drosophila S2 cells to measure S phase-coupled protein destruction via flow cytometry / Jean M. Davidson and Robert J. Duronio; title: 16. Assessing G₁-to-S-phase progression after genotoxic stress / Michael DeRan, Mary Pulvino, and Jiyong Zhao; title: 17. Indirect immunofluorescence for monitoring spindle assembly and disassembly in yeast / Jacob W.P. Keeling and Rita K. Miller; title: 18. Detecting recruitment of DNA damage response factors through the eChIP approach / Yucai Wang and Lei Li; title: 19. Methods to study cancer therapeutic drugs that target cell cycle checkpoints / Yun Dai and Steven Grant.