Sphingosine-1-phosphate
Subtitle: methods and protocols
By: edited by Alice Pébay, Kursad Turksen
Series: Methods in molecular biology -- 874; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 874.; Springer protocols
Publisher: Humana Press
Published: 2012
Place of publication: New York
Language: eng
Number of pages: 221
ISBN-13: 9781617797996; 9781617798009
Subjects: Sphingosine; Laboratory manuals; Sphingolipids
Notes: Includes bibliographical references and index.
Table of contents: title: Biochemical methods for quantifying sphingolipids : ceramide, sphingosine, sphingosine kinase-1 activity, and sphingosine-1-phosphate  -- Leyre Brizuela and Olivier Cuvillier; title: Isoform-selective assays for sphingosine kinase activity  -- Melissa R. Pitman, Duyen H. Pham, and Stuart M. Pitson; title: Quantification of sphingosine-1-phosphate and related sphingolipids by liquid chromatography coupled to tandem mass spectrometry  -- Constantin Bode and Markus H. Gräler; title: Cleanup method for mass spectrometry of sphingosine-1-phosphate in blood and solid tissues using a phosphate capture molecule  -- Jun-ichi Morishige, Tamotsu Tanaka, and Kiyoshi Satouchi; title: Immunohistochemical detection of sphingosine-1-phosphate and sphingosine kinase-1 in human tissue samples  -- Barbara Visentin, Gary Reynolds, and Roger Sabbadini; title: Assessment of sphingosine-1-phosphate activity in biological samples by receptor internalization and adherens junction formation  -- Hideru Obinata and Timothy Hla; title: High-throughput assays to measure intracellular Ca2+ mobilization in cells that express recombinant S1P receptor subtypes  -- William J. Valentine and Gabor Tigyi; title: Quantifying sphingosine-1-phosphate-dependent activation of the RhoGTPases  -- Matt D. Medlin, Joan M. Taylor, and Christopher P. Mack; title: Optimized protocols to analyze sphingosine-1-phosphate signal transduction pathways during acrosomal exocytosis in human sperm  -- Silvia A. Belmonte and Laila Suhaiman; title: Use of intravital microscopy and in vitro chemotaxis assays to study the roles of sphingosine-1-phosphate in bone homeostasis  -- Taeko Ishii ... [et al.]; title: Assessment of sphingosine-1-phosphate receptor expression and associated intracellular signaling cascades in primary cells of the human central nervous system  -- Veronique E. Miron ... [et al.]; title: Sphingosine-1-phosphate signaling in skeletal muscle cells  -- Elisabetta Meacci, Francesca Bini, and Chiara Battistini; title: Maintenance of human embryonic stem cells by sphingosine-1-phosphate and platelet-derived growth factor  -- Raymond C.B. Wong, Martin F. Pera, and Alice Pébay; title: Ceramide and sphingosine-1-phosphate signaling in embryonic stem cell differentiation  -- Erhard Bieberich; title: Sphingosine-1-phosphate signaling in neural progenitors  -- Phillip Callihan and Shelley B. Hooks; title: Utilizing sphingosine-1-phosphate to stimulate sprouting angiogenesis -- Shih-Chi Su and Kayla J. Bayless.