Comparison of the bacterial composition and structure in symptomatic and asymptomatic endodontic infections associated with root-filled teeth using pyrosequencing
Contributors: Al-Ahmad, Ali 1966- Verfasser; Elamin, Fadil Verfasser; Jonas, Daniel 1959- Verfasser; Karygianni, Lamprini 1981- Verfasser; Hellwig, Elmar 1954- Verfasser; Rehman, Ateequr Verfasser; Klinik für Zahnerhaltungskunde und Parodontologie; Institut für Umweltmedizin und Krankenhaushygiene; Albert-Ludwigs-Universität Freiburg
Publisher: Universität
Published: 2013
Place of publication: Freiburg
Language: eng
Subjects: Zahnkrankheit
Description: Abstract: Residual microorganisms and/or re-infections are a major cause for root canal therapy failure. Understanding of the bacterial content could improve treatment protocols. Fifty samples from 25 symptomatic and 25 asymptomatic previously root-filled teeth were collected from Sudanese patients with periradicular lesions. Amplified 16S rRNA gene (V1-V2) variable regions were subjected to pyrosequencing (FLX 454) to determine the bacterial profile. Obtained quality-controlled sequences from forty samples were classified into 741 operational taxonomic units (OTUs) at 3% dissimilarity, 525 at 5% dissimilarity and 297 at 10% dissimilarity, approximately corresponding to species-, genus- and class levels. The most abundant phyla were: Firmicutes (29.9%), Proteobacteria (26.1%), Actinobacteria (22.72%), Bacteroidetes (13.31%) and Fusobacteria (4.55%). Symptomatic patients had more Firmicutes and Fusobacteria than asymptomatic patients, while asymptomatic patients showed more Proteobacteria and Actinobacteria. Interaction of disease status and age was observed by two-way ANOSIM. Canonical correspondence analysis for age, tooth restoration and disease status showed a correlation of disease status with the composition and prevalence of different members of the microbial community. The pyrosequencing analysis revealed a distinctly higher diversity of the microbiota compared to earlier reports. The comparison of symptomatic and asymptomatic patients showed a clear association of the composition of the bacterial community with the presence and absence of symptoms in conjunction with the patients’ age
Notes: PLoS one. 8, 12 (2013), e84960, DOI 10.1371/journal.pone.0084960, issn: 1932-6203

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