Molecular cloning
Subtitle: a laboratory manual
By: Michael R. Green, Joseph Sambrook
Contributors: Sambrook, Joseph; Sambrook, Joseph
Edition: 4th ed.
Publisher: Cold Spring Harbor Laboratory Press
Published: 2012
Place of publication: Cold Spring Harbor, N.Y
Language: eng
Number of pages: 2000
ISBN-13: 9781936113415; 9781936113422
Subjects: Molecular cloning; Laboratory manuals
Description: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential. ... The theoretical and historical underpinnings of techniques are prominent features of the presentation throughout, information that does much to help trouble-shoot experimental problems. For the fourth edition of this classic work, the content has been entirely recast to include nucleic-acid based methods selected as the most widely used and valuable in molecular and cellular biology laboratories. Core chapters from the third edition have been revised to feature current strategies and approaches to the preparation and cloning of nucleic acids, gene transfer, and expression analysis. They are augmented by 12 new chapters which show how DNA, RNA, and proteins should be prepared, evaluated, and manipulated, and how data generation and analysis can be handled. The new content includes methods for studying interactions between cellular components, such as microarrays, next-generation sequencing technologies, RNA interference, and epigenetic analysis using DNA methylation techniques and chromatin immunoprecipitation. To make sense of the wealth of data produced by these techniques, a bioinformatics chapter describes the use of analytical tools for comparing sequences of genes and proteins and identifying common expression patterns among sets of genes. Building on thirty years of trust, reliability, and authority, the fourth edition of Molecular Cloning is the new gold standard--the one indispensable molecular biology laboratory manual and reference source. --Publisher description.
Notes: Rev. ed. of: Molecular cloning : a laboratory manual / Joseph Sambrook, David W. Russell. 2001.

Includes bibliographical references and index.