Cell biology assays
Subtitle: essential methods
By: edited by Gery Kreitzer, J.E. Celis
Publisher: Academic
Published: 2009
Place of publication: London
Language: eng
Number of pages: 286
ISBN-13: 9780123751522
Subjects: Technique; Laboratory Manuals; Cytological Techniques; Laboratory manuals; Cytology
Table of contents: title: Preface p. vii; title: List of Contributors p. ix; title: Membrane Protein Translocation Assays: Exocytosis, Endocytosis, Er-Golgi, Translocation Through the Er Permeabilized Epithelial Cells to Study Exocytic Membrane Transport p. 3; title: Studying Exit and Surface Delivery of Post-Golgi Transport Intermediates Using In Vitro and Live-Cell Microscopy-Based Approaches p. 13; title: Use of Permeabilized Mast Cells to Analyze Regulated Exocytosis p. 29; title: Cell Surface Biotinylation and Other Techniques for Determination of Surface Polarity of Epithelial Monolayers p. 39; title: Assays Measuring Membrane Transport in the Endocytic Pathway p. 53; title: Microsome-Based Assay for Analysis of Endoplasmic Reticulum to Golgi Transport in Mammalian Cells p. 63; title: Cotranslational Translocation of Proteins into Microsomes Derived from the Rough Endoplasmic Reticulum of Mammalian Cells p. 71; title: Loading Protein into Cells to Assess PM Function Syringe Loading: A Method for Assessing Plasma Membrane Function as a Reflection of Mechanically Induced Cell Loading p. 83; title: Protein Translocation Between Cytoplasm and Compartments Other than Plasma Membrane Protein Translocation into Mitochondria p. 97; title: Polarographic Assays of Mitochondrial Functions p. 107; title: Analysis of Nuclear Protein Import and Export in Digitonin-Permeabilized Cells p. 115; title: Heterokaryons: An Assay for Nucleocyto-plasmic Shuttling p. 127; title: Measuring Intracellular Signaling Events Using Fluorescence Microscopy Ca2+ as a Second Messenger: New Reporters for Calcium (Cameleons and Camgaroos) p. 139; title: Ratiometric Pericam p. 153; title: In Situ Cell Electroporation to Study of Signaling Pathways Dissecting Pathways; In Situ Electroporation for the Study of Signal Transduction and Gap Junctional Communication p. 165; title: In Situ Electroporation of Radioactive Nucleotides: Assessment of Ras Activity or 32P Labeling of Cellular Proteins p. 183; title: Tracking Individual Chromosomes with Integrated Arrays of lacop Sites and GFP-laci Repress.