Development of colorectal tumours in Apc Min/+ mice
Subtitle: effects on aryl hydrocarbon receptor expression.
Published: 2005
Language: eng
Number of pages: 120
Description: The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that mediates induction of many drug metabolizing enzymes including cytochrome P450s (CYP1A1, CYP1A2, CYP1B1). The AhR is over-expressed in certain types of tumours, but it is unknown whether it is also over-expressed in colorectal tumours. I attempted to determine if there are changes in AhR or AhR-regulated gene expression in adenomas from C57BL/6J-Apc Min/J mice as compared to matching tissue from C57BL/6J mice. There was a significant increase in AhR mRNA in tumours from the ileum as compared to matching normal tissue from control mice in two of the four  ApcMin/+ mice used. Mice which expressed increased AhR had a corresponding increase in expression of CYP1B1 and COX-2 mRNA. However, CYP1A1 expression was significantly decreased in all ileum tumors from all four ApcMin/+ mice. This work suggests that the AhR and CYP1B1 may be important as therapeutic targets against colorectal cancer.
Notes: Source: Masters Abstracts International, Volume: 44-02, page: 0830.

Thesis (M.Sc.)--University of Toronto, 2005.

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