Innate DNA and RNA recognition
Subtitle: methods and protocols
By: edited by Hans-Joachim Anders, Medizinische Klinik und Poliklinik IV, Maximilians Universität München, München, Germany, Adriana Migliorini, Helmholtz Zentrum München, German Research Center for Environmental Health, Institute for Diabetes and Regeneration Research (IDR), Munich, Germany
Series: Methods in Molecular Biology -- 1169; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 1169.; Springer protocols (Series)
Publisher: Humana Press
Published: 2014
Place of publication: New York
Language: eng
Number of pages: 195
ISBN-13: 9781493908813; 9781493908820
Subjects: Immune recognition; Nucleic acids; Laboratory manuals; RNA; Innate Immunity; Immunology; Cell receptors; DNA; Laboratory Manuals; Toll-Like Receptors
Notes: Includes bibliographical references and index.
Table of contents: title: Detection of RNA modifications by HPLC analysis and competitive ELISA  -- Gernot Nees, Andreas Kaufmann, and Stefan Bauer; title: Enzymatic synthesis and purification of a defined RIG-I ligand  -- Marion Goldeck ... [et al.]; title: Crystallization of mouse RIG-I ATPase domain : in situ proteolysis  -- Filiz Civril and Karl-Peter Hopfner; title: Isolation of RIG-I-associated RNAs from virus-infected cells  -- Andreas Schmidt ... [et al.]; title: Structure modeling of Toll-Like Receptors  -- Jing Gong and Tiandi Wei; title: Nucleic acid recognition in dendritic cells  -- Alexander Heiseke, Katharina Eisenächer, and Anne Krug; title: Viral nucleic acid recognition in human nonimmune cells : in vitro systems  -- Andrea Ribeiro and Markus Wörnle; title: Analysis of nucleic acid-induced nonimmune cell death in vitro  -- Simone Romoli and Adriana Migliorini; title: In vitro analysis of nucleic acid recognition in B lymphocytes  -- Saskia Ziegler and Isabelle Bekeredjian-Ding; title: Mapping of optimal CD8 T cell epitopes  -- Julia Roider, Thomas Vollbrecht, and Rika Draenert; title: Modular approach to suppression assays : TLR ligands, conditioned medium, and viral infection  -- Viktor H. Koelzer and David Anz; title: MicroRNA methodology : advances in miRNA technologies -- Theresa Kaeuferle ... [et al.]; title: Expression profiling by real-time quantitative polymerase chain reaction (RT-qPCR)  -- Maciej Lech and Hans-Joachim Anders; title: Evaluating the role of nucleic acid antigens in murine models of systemic lupus erythematosus  -- Amanda A. Watkins, Ramon G. B. Bonegio, and Ian R. Rifkin; title: Induction and analysis of nephrotoxic serum nephritis in mice  -- John M. Hoppe and Volker Vielhauer; title: Isolation of intratumoral leukocytes of TLR-stimulated tumor-bearing mice  -- Moritz Rapp, David Anz, and Max Schnurr; title: Bifunctional siRNAs for tumor therapy -- Fanny Matheis and Robert Besch.