RT-PCR protocols
Edition: 2nd ed. / edited by Nicola King.
Series: Methods in molecular biology -- 630; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 630.; Springer protocols
Publisher: Humana; Humana Press
Published: 2010
Place of publication: New York
Language: eng
Number of pages: 341
ISBN-13: 9781607616283; 9781607616290
Subjects: Polymerase chain reaction; Laboratory manuals; Reverse Transcriptase Polymerase Chain Reaction; Laboratory Manuals
Description: Once a tedious, highly skilled operation, reverse-transcription polymerase chain reaction (RT-PCR) has become a routine and invaluable technique used in most laboratories. In RT-PCR Protocols, Second Edition, expert researchers fully update the technologies presented in the popular previous edition, such as competitive RT-PCR, nested RT-PCR, RT-PCR from single cells, and RT-PCR for cloning. In addition, newer technologies are also explored, including multiplex RT-PCR, RT-LATE-PCR, and the greatly advanced field of real-time quantitative RT-PCR, while recent advances in creating the optimum RT-PCR reaction, e.g. RNA extraction, primer design, and reverse transcription, end the book with their indispensable input. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls. User friendly and up-to-date, RT-PCR Protocols, Second Edition acts as a handy companion to scientists from numerous diverse backgrounds who wish to explore further the marvels of gene expression.
Notes: Includes bibliographical references and index.

Also available online.
Table of contents: title: Single cell RT-PCR on mouse embryos : a general approach for developmental biology / Wolfgang Mann and Thomas Haaf; title: Poly(A) cDNA real-time PCR for indicator gene measurement in cancer / Melissa Oliveira-Cunha, Ajith K. Siriwardena, and Richard J. Byers; title: Transcriptome profiling of host-microbe interactions by differential display RT-PCR / Leong Wai Fook and Vincent T.K. Chow; title: Quantitative RT-PCR methods for mature microRNA expression analysis / Stephanie D. Fiedler, Martha Z. Carletti, and Lane K. Christenson; title: Detection of influenza A virus neuraminidase and PB2 gene segments by one step reverse transcription polymerase chain reaction / Alejandra Castillo Alvarez ... [et al.]; title: Detection and identification of CD46 splicing isoforms by nested RT-PCR / Anita Szalmás ... [et al.]; title: Simultaneous detection of bluetongue virus RNA, internal control GAPDH mRNA, and external control synthetic RNA by multiplex real-time PCR / Frank Vandenbussche, Elise Vandemeulebroucke, and Kris De Clercq; title: Detection of West Nile viral RNA from field-collected mosquitoes in tropical regions by conventional and real-time RT-PCR / Ana Silvia González-Reiche ... [et al.]; title: Detection of antisense RNA transcripts by strand-specific RT-PCR / Eric C.H. Ho, Michael E. Donaldson, and Barry J. Saville; title: RT-PCR amplification and cloning of large viral sequences / Xiaofeng Fan and Adrian M. Di Bisceglie; title: One-step RT-LATE-PCR for mRNA and viral RNA detection and quantification / Cristina Hartshorn and Lawrence J. Wangh; title: Changes in gene expression of caveolin-1 after inflammatory pain using quantitative real-time PCR / Fiza Rashid-Doubell; title: Real-time quantitative reverse transcriptase polymerase chain reaction / Hongxin Fan and Ryan S. Robetorye; title: The use of comparative quantitative RT-PCR to investigate the effect of cysteine incubation on GPx1 expression in freshly isolated cardiomyocytes / Nicola King; title: The renaissance of competitive PCR as an accurate tool for precise nucleic acid quantification / Lorena Zentilin and Mauro Giacca; title: Skeletal muscle RNA extraction in preparation for RT-PCR / Janelle P. Mollica; title: Reverse transcription of the ribonucleic acid : the first step in RT-PCR assay / Fadia Haddad and Kenneth M. Baldwin; title: Primer design for RT-PCR / Kelvin Li and Anushka Brownley; title: Hot start PCR / Natasha Paul, Jonathan Shum, and Tony Le; title: Real-time RT-PCR for automated detection of HIV-1 RNA during blood donor screening / Jens Müller.