Quantitative study of the phagosome maturation pathway and the alteration of this pathway by Salmonella enterica serovar Typhimurium through the use of Rab GTPases.
Published: 2006
Language: eng
Number of pages: 105
ISBN-13: 9780494161616
Description: Upon invasion of epithelial cells, Salmonella enterica serovar Typhimurium reside in the Salmonella-containing vacuole (SCV). The bacteria manipulate the trafficking of this compartment to avoid degradation by inhibiting interactions with late endosomes and lysosomes. This study looked to characterize the series of interactions that take place between the SCV and the host cell. To do this, the kinetics of SCV interactions with cellular trafficking regulators, Rab GTPases, was studied over the initial three hours of infection. These interactions were compared to interactions experienced by a model phagosome that matures to a phagolysosome. Comparing the differences between these two vacuoles resulted in the discovery of novel regulators of phagocytic maturation and new interactions between the SCV and the endocytic machinery, including an interaction with the endocytic recycling compartment. These findings provide insight into the nature phagosome maturation as well as SCV modifications that allow S. Typhimurium to replicate intracellularly.
Notes: Source: Masters Abstracts International, Volume: 44-06, page: 2712.

Thesis (M.Sc.)--University of Toronto, 2006.

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