Self-assembled elastin-like polypeptide particles.
Published: 2006
Language: eng
Number of pages: 217
ISBN-13: 9780494210383
Description: In this work, the self-assembly of a recombinant elastin-based block copolymer was investigated. The particle formation and dynamic behavior were characterized using inverted microscopy and dynamic light scattering. The morphology and stability were evaluated using scanning and transmission electron microscopy. Above a critical temperature the molecules self-assembled into a bimodal distribution of nano and micron-sized particles. The larger particles increased in size through coalescence. Micron-sized particle formation appeared largely reversible, although a self-assembly/disassembly hysteresis was observed. At high polyethylene glycol (PEG) concentrations particle coalescence and settling were reduced, particle stability seemed enhanced, and PEG coated the particles. Particle stabilization was also achieved through covalent crosslinking using glutaraldehyde. The particles captured a hydrophobic fluorescent probe with high efficiency and the data suggested a gradual release. This study laid the foundation for further particle size and stability optimization, and showed promise for particulate drug carriers using this family of elastin-based block copolymers.
Notes: Source: Masters Abstracts International, Volume: 45-03, page: 1566.

Thesis (M.A.Sc.)--University of Toronto, 2006.

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