Transmembrane signaling protocols
By: edited by Hydar Ali and Bodduluri Haribabu.
Contributors: Ali, Hydar.; Haribabu, Bodduluri.
Edition: 2nd ed.
Series: Methods in molecular biology ;; 332; Methods in molecular biology (Clifton, N.J.) ;; 332.
Publisher: Humana Press; Humana
Published: 2006
Place of publication: Totowa, N.J
Language: eng
Number of pages: 376
Subjects: Cellular signal transduction -- Laboratory manuals; Signal Transduction -- Laboratory Manuals; GTP-Binding Proteins -- physiology -- Laboratory Manuals
Genres: Laboratory manuals.; Laboratory Manuals.
Description: Since the publication of the first edition, new advances have increased the ability to understand transmembrane signaling processes in greater detail. The human genome has been completely sequenced as well as new methods have been developed for mircroarrays and proteomics analyzing global changes in gene expression and protein profiles. Updated to include these new methods, the 2nd edition also includes comprehensive detailed methods, studies in genetically tractable systems, fluorescence microscopy in live single cells, ex vivo analysis of primary cells from transgenic mice, and genomic and proteomic approaches to the analysis of transmembrane signaling events.
Notes: Includes bibliographical references and index.
Table of contents: title: Transmembrane signaling by G protein-coupled receptors / Louis M. Luttrell; title: Cross-talk coregulation mechanisms of G protein-coupled receptors and receptor tyrosine kinases / Kanchana Nataranjan and Bradford C. Berk; title: Protein/protein interactions in signaling pathways / Bruce J. Mayer; title: Biological role of the CXCR4-SDF-1 axis in normal human hematopoietic cells / Marcin Majka and Mariusz Z. Ratajczak; title: Functional expression of CXCR4 in Saccharomyces cerevisiae for the development of powerful tools for pharmacological characterization of CXCR4 / Zi-xuan Wang, James R. Broach, and Stephen C. Peiper; title: Characterization of constitutively active mutants of G protein-coupled receptors / Jean-Marc Navenot, Zi-xuan Wang, and Stephen C. Peiper; title: G protein-coupled receptor dimerization and signaling / Mario Mellado ... [et al.]; title: Real-time analysis of G protein-coupled receptor signaling in live cells / Venkatakrishna R. Jala and Bodduluri Haribabu; title: Isolation of membrane lipid rafts and signaling complexes / Kathleen Boesze Battaglia; title: Methods for the study of signaling molecules in membrane lipid rafts and caveolae / Rennolds S. Ostrom and Paul A. Insel; title: Bioluminescence resonance energy transfer to monitor protein/protein interactions / Tarik Issad and Ralf Jockers; title: Identification of interacting proteins using the yeast two-hybrid screen / Kelly L. Jordan-Sciutto and Marshall B. Montgomery; title: Analysis of PDZ domain interactions using yeast two-hybrid and coimmunoprecipitation assays / Hyun Woo Lee, Jaewon Ko, and Eunjoon Kim; title: Clustering assay for studying the interaction of membrane proteins with PDZ domain proteins / Jaewon Ko and Eunjoon Kim; title: Mammalian cell microinjection assay to study the function of rho family guanosine triphosphatases / Ritu Garg and Anne J. Ridley; title: Affinity-based assay of Rho guanosine triphosphatase activation / Mary Stofega, Celine DerMardirossian, and Gary M. Bokoch; title: Assay for phospholipase d in cell-free systems / Shanker S. Iyer and David Kusner; title: Reconstitution system based on cytosol-depleted cells to study regulation of phospholipase D / Amanda Fensome-Green and Shamshad Cockcroft; title: Analysis of global gene expression profiles activated by chemoattractant receptors / Fernando O. Martinez and Massimo Locati; title: Genetic reconstitution of bone marrow for the study of signal transduction ex vivo / Martha S. Jordan; title: Proteomic analysis of human neutrophils / George Lominadze ... [et al.].