Characterization of gene expression profiles during differentiation, lipopolysaccharide stimulation, and heat shock repression in human U937 monocyte cells.
Published: 2004
Language: eng
Number of pages: 140
Description: The U937 cell line represents a class of immature monocytes capable of induced terminal differentiation, generating an immune response to lipopolysaccharide (LPS), and protecting itself from stresses brought about by heat. As it is an often used model cell line, we sought to characterize U937 gene expression profiles during the above mentioned processes as well as examining the resulting profile of LPS stimulation concomitant with heat stress, using microarray technology. Treatment with phorbol-12-myristate-13-acetate (PMA) led to the induction and downregulation of a unique set of genes that differed (but not completely) at 6h and 24h of differentiation. Similarly, microarray analyses revealed a novel set of LPS-inducible as well as downregulated genes. Concomitant heat shock and LPS exposure demonstrated the repression of almost all LPS-inducible genes. Further, heat shock treatment alone induced several novel genes in addition to the classic heat shock proteins, Hsp70 and Hsp40.
Notes: Adviser:  Tim Westwood.

Thesis (M.Sc.)--University of Toronto, 2004.

Electronic version licensed for access by U. of T. users.

Source: Masters Abstracts International, Volume: 43-03, page: 0784.

MICR copy on microfiche (2 microfiches).