REGULATION OF HEPATIC INTERMEDIARY METABOLIC PATHWAYS DURING HIV INFECTION (IMMUNE DEFICIENCY, AIDS).
Contributors: University of California, San Francisco.
Published: 1990
Number of pages: 200
Subjects: Health Sciences, Nursing.; Health Sciences, Nutrition.
Description: The purpose of this study was to describe regulation of hepatic intermediary metabolism during different stages of HIV infection. Pathways of hepatic glucose utilization during fasting and refeeding were measured using infusions of 1-dl glucose and acetaminophen (glucuronide probe) in three groups of subjects: healthy volunteers (n = 9), asymptomatic HIV infection (n = 7), and AIDS (n = 7). A method for determining fraction of hepatic gluconeogenesis (GNG) and UDP-gluconeogenesis (UDP-GNG) from phosphoenolpyruvate during fasting using infusions of 13C acetate in conjunction with oral sulfamethoxazole (acetyl probe) and acetaminophen infusions was also tested.

In all groups (N = 23) the indirect pathway of hepatic glucose utilization predominated during fasting. Healthy volunteers and asymptomatic HIV subjects tended to switch to the direct glucose pathway with refeeding, while the AIDS group persisted in the indirect pathway. Catabolic subjects with AIDS who were refed by the oral route showed almost no direct hepatic glucose uptake. Large effects of group membership were observed during fasting and refeeding.

Fraction of hepatic GNG and UDP-GNG were not able to be determined in the limited samples available to the present study due to the error sensitivity of the method employed. Large effects of group membership on isotopic incorporation parameters suggest, however, that group differences in hepatic GNG and UDP-GNG may be detectable in future studies involving larger samples.
Notes: Source: Dissertation Abstracts International, Volume: 51-08, Section: B, page: 3779.

Thesis (PH.D.)--UNIVERSITY OF CALIFORNIA, SAN FRANCISCO, 1990.

School code: 0034.