Isolation and characterization of a human WAC gene differentially expressed in long-term bone marrow culture.
Published: 2004
Language: eng
Number of pages: 112
Description: hWAC is a novel gene, differentially expressed in long-term bone marrow cultures (LTBMCs), in which hematopoiesis was activated by medium changes. We hypothesize that hWAC might be uniquely involved in proliferation and differentiation of hematopoietic stem cells. Located on chromosome 10, this approximately 88-kb gene is highly conserved in mammals compared to other species. There were four hWAC transcripts found ranging from 3--4 kb. The level of hWAC transcription is higher (a) in bone marrow and some non-hematopoietic tissues than that in kidney, thymus and spleen, (b) in lymphoid than in myeloid cells from bone marrow, and (c) in stromal cells than HSCs in adherent layers of LTBMCs. Multiple protein domains were found in putative hWAC proteins. Our studies showed that one hWAC isoform was distributed into the nucleus, while another remained in both cytoplasm and nucleus in different cell types. However, the function of hWAC remains unknown.
Notes: Adviser:   Margaret Hough.

Thesis (M.Sc.)--University of Toronto, 2004.

Electronic version licensed for access by U. of T. users.

Source: Masters Abstracts International, Volume: 43-03, page: 0776.

MICR copy on microfiche (2 microfiches).