Advanced Protocols for Animal Transgenesis
Subtitle: An ISTT Manual
By: edited by Shirley Pease, Thomas L. Saunders
Contributors: Saunders, Thomas L.
Series: Springer Protocols Handbooks; Springer protocols (Series)
Publisher: Springer
Published: 2011
Language: eng
Number of pages: 669
ISBN-13: 9783642207921
Subjects: Animal genetics; Life sciences; Transgenic organisms
Description: This laboratory manual, published in cooperation with the International Society for Transgenic Technology (ISTT), provides almost all current methods that can be applied to the creation and analysis of genetically modified animals. The chapters have been contributed by leading scientists who are actively using the technology in their laboratories. Based on their first-hand experience the authors also provide helpful notes and troubleshooting sections. Topics range from standard techniques, such as pronuclear microinjection of DNA, to more sophisticated and modern methods, such as the derivation and establishment of embryonic stem (ES) cell lines, with defined inhibitors in cell culture medium. In addition, related topics with relevance to the field are addressed, including global web-based resources, legal issues, colony management, shipment of mice and embryos, and the three R's: refinement, reduction and replacement.
Table of contents: title: Karen S. Canady: Patent and licensing issues in transgenic technology; title: Llui s Montoliu: Global Resources: Including Gene Trapped ES Cell Clones: Is Your Gene Already Knocked Out?; title: Eduardo Molto , Cristina Vicente-Garci a and Llui s Montoliu: Designing Transgenes for Optimal Expression; title: Thomas L. Saunders: Gene Targeting Vector Design for Embryonic Stem Cell Modifications; title: Thomas J. Fielder and Lluis Montoliu: Transgenic Production Benchmarks; title: Katja Becker and Boris Jerchow: Generation of Transgenic Mice by Pronuclear Microinjection; title: Se verine Me noret, Se verine Remy, et al : Generation of Transgenic Rats using Microinjection of Plasmid DNA or Lentiviral vectors; title: Almudena Ferna ndez, Diego Mun oz and Llui s Montoliu: Generation of Transgenic Animals by Use of YACs; title: Michael G. Zeidler, Margaret L. Van Keuren, and Thomas L. Saunders: BAC Transgenes, DNA Purification, and Transgenic Mouse Production; title: Carlos Lois: Generation of Transgenic Animals with Lentiviral Vectors; title: Aron Geurts, Lajos Mates, Darius Balciunas: Vertebrate Transgenesis by Transposition; title: Karen M. Chapman and others: Rat Spermatogonial Stem Cell Mediated Gene Transfer; title: Sayaka Wakayama, Nguyen Van Thuan and Teruhiko Wakayama: Mouse Cloning by Nuclear Transfer; title: Elizabeth D. Hughes and Thomas L. Saunders: Gene Targeting in Embryonic Stem Cells; title: Wojtek Auerbach and Anna B. Auerbach: The Importance of Mouse ES Cell Line Selection; title: Marina Gertsenstein: Tetraploid Complementation Assay; title: Elizabeth Williams and others: Combining ES cells with Embryos; title: Kristina Nagy and Jennifer Nichols: Derivation of Murine ES Cell Lines; title: Ping Li and others: Rat Embryonic Stem Cell Derivation and Propagation; title: Han Li and others: Induced pluripotency: generation of iPS cells from mouse embryonic fibroblasts; title: Anna B. Auerbach, Peter J. Romanienko, and Willie H. Mark: The Preparation and Analysis of DNA for Use in Transgenic Technology; title: Karen Brennan: Colony Management; title: B. Pintado and J Hourcade: Cryopreservation; title: Shirley Pease: Shipment of Mice and Embryos; title: J.M. Sztein, R.J. Kastenmayer, K.A. Perdue: Pathogen Free Mouse Rederivation by IVF, Natural Mating and Hysterectomy; title: Jan Parker-Thornburg: Refinement, Reduction and Replacement.