YAC protocols
By: edited by Alasdair MacKenzie
Contributors: MacKenzie, Alasdair
Edition: 2nd ed
Series: Methods in molecular biology -- 349; Methods in molecular biology (Clifton, N.J.) --  349
Publisher: Humana Press
Published: 2006
Place of publication: Totowa, N.J
Language: eng
Number of pages: 203
Subjects: Molecular cloning -- Methods; Genetic vectors; Yeast fungi -- Genetic engineering; Saccharomyces cerevisiae
Genres: Methods
Description: This completely revised and updated edition takes advantage of the many new advances in biology since the publication of the first edition, including the sequencing of the human genome, the genomes of a wide variety of other organisms, and the increased use of transgenic animals for understanding the molecular basis of human and animal disease. It also provides a much-needed update on the new techniques currently employed and to help redefine and illustrate the important roles still to be palyed by yeast artificial chromosome (YAC) technologies in the postgenomic age.
Notes: Includes bibliographical references and index
Table of contents: title: Markers, selection, and media in yeast artificial chromosomes cloning / David Markie; title: Construction of yeast artificial chromosomes libraries from pathogens and nonmodel organisms / Cecilia P. Sanchez and Michael Lanzer; title: Analysis and screening of yeast artificial chromosomes libraries by filter hybridization / Cecilia P. Sanchez and Michael Lanzer; title: Analysis of yeast artificial chromosome DNA by restriction digestion, southern blotting nucleic acid hybridization and polymerase chain reaction / Sylvia Vasiliou and John Peter Quinn; title: Postgenomic bioinformatic analysis of yeast artificial chromosomes sequence / Kerry Miller, Scott Davidson, and Alasdair MacKenzie; title: Transformation of yeast using bio-active beads with surface-immobilized yeast artificial chromosomes / Shigeki Kawakami ... [et al.]; title: Modification and amplification of yeast artificial chromosomes / Sanbing Shen, Anthony Harmar, and Nick Hastie; title: Modifying yeast artificial chromosomes to generate Cre/LoxP and FLP/FRT site-specific deletions and inversions / Gabriela G. Loots; title: Selective isolation of large chromosomal regions by transformation-associated recombination cloning for structural and functional analysis of mammalian genomes / Natalay Kouprina, Vladimir N. Noskov, and Vladimir Larionov; title: A polymerase chain reaction-mediated yeast artificial chromosomes-splitting technology for generating targeted yeast artificial chromosomes subclones / Yeon-Hee Kim ... [et al.]; title: Genomic reconstruction by serial mitotic recombination of yeast artificial chromosomes / David Markie, Emma Jones, and Jiannis Ragoussis; title: Targeting vector construction by yeast artificial chromosome modification / Peter J. Murray; title: Production of yeast artificial chromosomes transgenic mice by pronuclear injection of one-cell embryos / Alasdair MacKenzie; title: Generation of yeast artificial chromosomes transgenic mice by intracytoplasmic sperm injection / Pedro N. Moreira ... [et al.]; title: Establishment of cell lines that exhibit correct ontogenic stage-specific gene expression pprofiles from tissues of yeast artificial chromosome transgenic mice using chemically induced growth signals / C. Anthony Blau and Kenneth R. Peterson; title: Application of yeast artificial chromosomes in fluorescence in situ hybridization / Thomas Liehr; title: Contribution of yeast artificial chromosome-based physical maps to the final assembly of the trypanosoma cruzi genome / Márcia R.M. Santos ... [et al.]