Gene mapping, discovery, and expression
Subtitle: methods and protocols
By: edited by Minou Bina.
Contributors: Bina, Minou.
Series: Methods in molecular biology ;; v. 338
Publisher: Humana Press
Published: 2006
Place of publication: Totowa, N.J
Language: eng
Number of pages: 334
Subjects: Gene mapping -- Methodology; Gene mapping -- Data processing; Genetics -- Technique; Genetic expression; Chromosome Mapping -- methods -- Laboratory Manuals; Databases, Nucleic Acid -- Laboratory Manuals; Gene Expression Profiling -- methods -- Laboratory Manuals; Microarray Analysis -- methods -- Laboratory Manuals
Genres: Laboratory Manuals.
Description: A collection of cutting-edge computational tools and experimental techniques to study how genes are regulated, and to reconstruct the regulatory networks through which various cell-types are produced. On the computational side, web-based technologies to localize genes, to access and retrieve data from microarray databases, to conduct comparative genomics, and to discover the potential genomic DNA that may control the expression of protein-coding genes. Detailed experimental techniques described include methods for studying chromatin structure and allele-specific gene expression, methods for high-throughput analysis to characterize the transcription factor binding elements, and methods for isolating and identifying proteins that interact with DNA.
Notes: Includes bibliographical references and index.
Table of contents: title: Use of genome browsers to locate your favorite genes / Minou Bina; title: Methods for identifying and mapping recent segmental and gene duplications in eukaryotic genomes / Razi Khaja ... [et al.]; title: Identification and mapping of paralogous genes on a known genomic DNA sequence / Minou Bina; title: Quantitative DNA fiber mapping in genome research and construction of physical maps / Heinz-Ulrich G. Weier and Lisa W. Chu; title: Prins for mapping single copy genes / Avirachan T. Tharapel and Stephen S. Wachtel; title: Vista family of computational tools for comparative analysis of DNA sequences and whole genomes / Inna Dubchak and Dmitriy V. Ryaboy; title: Computational prediction of CIS-regulatory modules from multispecies alignments using Galaxy, table browser, and GALA / Laura Elnitski, David King, and Ross C. Hardison; title: Comparative promoter analysis in vertebrate genomes with the CORG workbench / Christoph Dieterich and Martin Vingron; title: CIS-regulatory region analysis using BEARR / Vinsensius Berlian Vega; title: A database of 9-mers from promoter region of human protein-coding genes / Minou Bina, Phillip Wyss, and Syed Rehan Shah; title: A program toolkit for the analysis of regulatory regions of genes / Phillip Wyss, Sheryl A. Lazarus, and Minou Bina; title: Analysis of allele-specific gene expression / Julian Charles Knight; title: Construction of microrna expression vector in mammalian cells / Yoko Fukuda, Hiroaki Kawasaki, and Kazunari Taira; title: Mining microarray data at NCBI's gene expression omnibus (GEO) / Tanya Barrett and Ron Edgar; title: The Stanford microarray database: a user's guide / Jeremy Gollub, Catherine A. Ball, and Gavin Sherlock; title: Detecting nucleosome ladders on unique DNA sequences in mouse liver nuclei / Tomara J. Fleury, Alfred Cioffi, and Arnold Stein; title: DNA methyltransferase probing of DNA/protein interactions / Scott A. Hoose and Michael P. Kladde; title: Protein binding microarrays (PBMS) for the rapid, high-throughput characterization of the sequence specificities of DNA binding proteins / Michael F. Berger and Martha L. Bulyk; title: Quantitative profiling of protein-DNA binding on microarrays / Jiannis Ragoussis, Simon Field, and Irina A. Udalova; title: Analysis of protein-DNA binding by streptavidin-agarose pulldown / Kenneth K. Wu; title: Isolation and mass spectrometry of specific DNA-binding proteins / Mariana Yaneva and Paul Tempst; title: Isolation of transcription factor complexes by in vivo biotinylation tagging and direct binding to streptavidin beads / Patrick Rodriguez ... [et al.].