Genotypic/phenotypic analysis of endothelial progenitor cells in idiopathic pulmonary arterial hypertension and hereditary hemorrhagic telangiectasia.
Published: 2006
Language: eng
Number of pages: 132
ISBN-13: 9780494212585
Description: Introduction. Idiopathic pulmonary artenal hypertension (IPAH) and hereditary hemorrhagic telangiectasia (HHT) result from mutations TGF-beta superfamily receptors. Circulating endothelial progenitor cells (EPCs) have been shown to play an important role in vascular homeostasis and repair. Mutations in TGF-beta receptors may result in EPC dysfunction and alter their reparative potential. Methods. EPCs were isolated from controls, patients with IPAH (genotype unknown), familial PAH (FPAH, known BMPRII mutations) and HHT. Quantification of circulating EPC levels was performed immediately following isolation, remaining cells were assayed following a 7-day differential culture for phenotypic and functional abnormalities.  Results. A significant increase in circulating cells with the capacity to develop into EPCs was found in all patient groups, whereas, a significant decrease in differentiation capacity was observed in EPCs following differential culture. Functional assays demonstrated increased apoptotic rates in EPCs derived from IPAH and FPAH patients, while EPCs derived from HHT patients demonstrated impaired gene expression. Conclusions. EPCs, derived from patients vascular diseases associated with TGF-beta mutations, demonstrated abnormalities in function and phenotype suggestive of reduced endothelial differentiation and impaired regenerative capacity.
Notes: Source: Masters Abstracts International, Volume: 45-03, page: 1356.

Thesis (M.Sc.)--University of Toronto, 2006.

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