Disruption of imprinted transcription regulation of the  Mash2 gene by targeted DNA insertion.
Published: 2004
Language: eng
Number of pages: 88
Description: Mash2 is an imprinted gene, expressed only from the maternal but not the paternal allele. When transmitted maternally, a loss-of-function mutation or a hypomorphic mutation in Mash2 results in embryonic lethality at midgestation. Recently we discovered that when a Pgk-neo-pA  cassette was inserted 4.5kb downstream of Mash2 and inherited paternally, it could rescue the lethal maternal hypomorphic allele. The hypothesis was that the otherwise silent paternal Mash2  allele was expressed; therefore imprinting was relaxed. To study the site-specificity and sequence-specificity aspects of the rescue phenomenon, I developed a coherent system consisting of several different modified  Mash2 alleles by targeting different insertion cassettes into two different sites. Abilities of these modified alleles to rescue the lethal phenotype were studied. Results showed that the rescue phenomenon was specific to the insertion site 4.5kb downstream of Mash2. This opens the possibility that some imprinting regulatory elements silencing the paternal  Mash2 allele were interrupted.
Notes: Adviser:  Andras Nagy.

Thesis (M.Sc.)--University of Toronto, 2004.

Electronic version licensed for access by U. of T. users.

Source: Masters Abstracts International, Volume: 42-06, page: 2115.

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