Tumor necrosis factor
Subtitle: methods and protocols
By: edited by Angelo Corti and Pietro Ghezzi.
Contributors: Corti, Angelo.; Ghezzi, P.
Series: Methods in molecular medicine -- 98.
Publisher: Humana Press
Published: 2004
Place of publication: Totowa, N.J
Language: eng
Number of pages: 279
Subjects: Tumor necrosis factor -- Laboratory manuals
Genres: Laboratory manuals.
Description: Because tumor necrosis factor-a (TNF-a) plays a pivotal role in the regulation of homeostasis and inflammatory immune responses, it offers valuable research opportunities to develop new drugs for the treatment of a wide range of disorders, including cancer, septic shock, rheumatoid arthritis, and other inflammatory diseases. In Tumor Necrosis Factor: Methods and Protocols, well-versed experimentalists survey the basic and translational research being conducted in this field and describe in detail the methods they have developed for TNF production, characterization, mutagenesis, and detection in biological specimens. They also provide several in vitro assays and animal models for studying the role of TNF in various TNF-related diseases and in cancer. The protocols presented follow the successful Methods in Molecular Medicine™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, Tumor Necrosis Factor: Methods and Protocols offers molecular and cellular biologists, pharmacologists, and toxicologists a diverse set of productive, cutting-edge tools for illuminating the pathophysiological roles of TNF in disease and for identifying new drugs.
Notes: Includes bibliographical references and index.
Table of contents: title: Tumor necrosis factor as a pharmacological target / Pietro Ghezzi and Anthony Cerami; title: Production and characterization of recombinant human and murine TNF / Flavio Curnis and Angelo Corti; title: Purification of TNF binding proteins / Hartmut Engelmann, Dani Aderka, and David Wallach; title: Production and characterization of receptor-specific TNF muteins / Paul Ameloot and Peter Brouckaert; title: TNF[alpha] promoter polymorphysms / Silvia Fargion ... [et al.]; title: Detection of TNF and TNF receptor mRNA in cells and tissues / Roberto Furlan ... [et al.]; title: Immunohistochemical localization of TNF[alpha] and its receptors in the rodent central nervous system / Paolo Bigini and Tiziana Mennini; title: TNF signaling: key protocols / Marta Muzio and Simona Saccani; title: More than one way to die: methods to determine TNF-induced apoptosis and necrosis / Tom Vanden Berghe ... [et al.]; title: Assessment of TNF[alpha]-induced endothelial damage through the loss of its barrier function / Elisabetta Ferrero; title: Genetic engineering in the mouse: tuning TNF/TNFR expression / Eleni Douni, Maria Alexiou, and George Kollias; title: TNF[alpha] in experimental diseases of the CNS / Roberto furlan ... [eta l.]; title: In vivo vascular leakage assay / Maria Elena Ferrero; title: Animal models of endotoxic shock / Pia Villa and Pietro Ghezzi; title: Collagen-induced arthritis as a model for rheumatoid arthritis / Richard O. Williams; title: Miocardial infarction: animal models / Stefano Chimenti ... [et al.]; title: Tumor vascular therapy with TNF: critical review on animal models / Timo L.M. ten Hagen and Alexander M.M. Eggermont; title: Strategies for improving the anti-neoplastic activity of TNF by tumor targeting / Angelo Corti.