Cervical cancer
Subtitle: methods and protocols
By: edited by Daniel Keppler, Athena W. Lin
Contributors: Springer Science+Business Media
Series: Methods in molecular biology -- 1249; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 1249,; Springer protocols (Series)
Publisher: Humana Press
Published: 2014
Place of publication: New York
Language: eng
Number of pages: 413
ISBN-13: 9781493920129; 9781493920136
Copyright date: 2015
Subjects: Methods; Cancer; Laboratory Manuals; Uterine Cervical Neoplasms; Cervix uteri; Human Papillomavirus DNA Tests
Description: Representing the most relevant procedures and technologies aiding the advance of the field of HPV-mediated carcinogenesis of the cervix and other anatomical regions of squamocolumnar transition, such as the anorectum, penis, and oropharynx, Cervical Cancer: Methods and Protocols compiles a detailed collection of practical chapters. The first half of the book covers HPV types, pathogenesis of cervical cancer (CxCA), prevention, and novel potential drug targets, while the second half explores pathology, genomics, modeling of CxCA, and experimental therapeutic strategies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and vital, Cervical Cancer: Methods and Protocols serves as a valuable resource to both bench scientists and clinicians who step into the realm of high-risk HPVs and CxCA for the first time or those who wish to learn novel approaches or expand their toolbox for the study of CxCA.
Notes: Includes bibliographical references and index.
Table of contents: title: Evolution and classification of oncogenic human papillomavirus types and variants associated with cervical cancer  -- Zigui Chen, Luciana Bueno de Freitas, and Robert D. Burk; title: Real-time PCR approach based on SPF10 primers and the INNO-LiPA HPV genotyping extra assay for the detection and typing of human papillomavirus  -- M. Isabel Micalessi, Gaëlle A. Boulet, and Johannes Bogers; title: Replication of human papillomavirus in culture  -- Eric J. Ryndock, Jennifer Biryukov, and Craig Meyers; title: HPV binding assay to laminin-332/integrin [alpha]6[beta]4 on human keratinocytes  -- Sarah A. Brendle and Neil D. Christensen; title: Methods to assess the nucleocytoplasmic shuttling of the HPV E1 helicase and its effects on cellular proliferation and induction of a DNA damage response  -- Michaël Lehoux, Amélie Fradet-Turcotte, and Jacques Archambault; title: Genetic methods for studying the role of viral oncogenes in the HPV life cycle  -- Jason M. Bodily; title: Robust HPV-18 production in organotypic cultures of primary human keratinocytes  -- Hsu-Kun Wang, Thomas R. Broker, and Louise T. Chow; title: High-throughput cellular assay to quantify the p53- degradation activity of E6 from different human papillomavirus types  -- David Gagnon and Jacques Archambault; title: Retroviral expression of human cystatin genes in HeLa cells  -- Crystal M. Diep, Gagandeep Kaur, Daniel Keppler, and Athena W. Lin; title: Molecular analysis of human papillomavirus virus-like particle activated langerhans cells in vitro  -- Andrew W. Woodham, Adam W. Woodham, Adam B. Raff, Diane M. Da Silva, and W. Martin Kast; title: Selective silencing of gene target expression by siRNA expression plasmids in human cervical cancer cells  -- Oscar Peralta-Zaragoza [and eleven others]; title: Silencing of E6/E7 expression in cervical cancer stem- like cells  -- Wenyi Gu, Nigel McMillan, and Chengzhong Yu; title: Two-step procedure for evaluating experimentally induced DNA damage : texas red and comet assays  -- Gina J. Ferris, Lauren B. Shunkwiler, and Charles A. Kunos; title: Measurement of deubiquitinating enzyme Activity via a suicidal HA-Ub-VS probe  -- Colleen Rivard and Martina Bazzaro; title: Immunocytochemical analysis of the cervical pap smear  -- Terry K. Morgan and Michelle Berlin; title: Diagnosis of HPV-negative, gastric-type adenocarcinoma of the endocervix  -- Edyta C. Pirog; title: Targeting of the HPV-16 E7 protein by RNA aptamers  -- Julia Dolores Toscano-Garibay, María Luisa Benítez-Hess, and Luis Marat Alvarez-Salas; title: Use of MYBL2 as a novel candidate biomarker of cervical cancer  -- Cara M. Martin [and others]; title: Fixation methods for the preservation of morphology, RNAs, and proteins in paraffin-Embedded human cervical cancer cell xenografts in mice  -- Yoko Matsuda and Toshiyuki Ishiwata; title: Assessment of the HPV DNA methylation status in cervical lesions  -- Mina Kalantari and Hans-Ulrich Bernard; title: MeDIP-on-chip for methylation profiling  -- Yaw-Wen Hsu, Rui-Lan Huang, and Hung-Cheng Lai; title: Use of DBD-FISH for the study of cervical cancer progression  -- Elva I. Cortés-Gutiérrez [and four others]; title: Quantitative and high-throughput assay of human papillomavirus DNA replication  -- David Gagnon, Amélie Fradet-Turcotte, and Jacques Archambault; title: Native human papillomavirus production, quantification, and infectivity analysis  -- Jennifer Biryukov, Linda Cruz, Eric J. Ryndock, and Craig Meyers; title: Functional analysis of HPV-like particle-activated langerhans cells in vitro  -- Lisa Yan, Andrew W. Woodham, Diane M. Da Silva, and W. Martin Kast; title: Assessment of the radiation sensitivity of cervical cancer cell lines  -- Mayil Vahanan Bose and Thangarajan Rajkumar; title: Mouse model of cervicovaginal papillomavirus infection  -- Nicolas Çuburu, Rebecca J. Cevio, Cynthia D. Thompson, and Patricia M. Day; title: Establishment of orthotopic primary cervix cancer xenografts  -- Naz Chaudary, Karolina Jaluba, Melania Pintilie, and Richard P. Hill; title: Generation of K14-E7/[delta]N87[beta] cat double transgenic mice as a model of cervical cancer -- Gülay Bulut and Aykut Üren.