Molecular Biology Techniques
Subtitle: An Intensive Laboratory Course
Publisher: Academic Press
Published: January 15, 1999
Language: eng
Number of pages: 234
ISBN-13: 9780125839907
First sentence: The purpose of CsCl-ethidium bromide density gradient centrifugation is to separate supercoiled (covalently closed circular) plasmid DNA from other components present in lysates of bacterial cells: linear DNA (chromosomal fragments), nicked plasmid DNA (which lacks supercoils), RNA, proteins, and carbohydrates.