Cardiomyocytes
Subtitle: Methods and Protocols
By: edited by Gary R. Skuse and Maureen C. Ferran
Series: Methods in molecular biology -- 1299; Springer protocols; Methods in molecular biology -- 1299,; Springer protocols (Series)
Publisher: Humana Press; Springer
Published: 2015
Place of publication: New York
Language: eng
Number of pages: 216
ISBN-13: 9781493925711; 9781493925728
Copyright date: 2015
Subjects: Heart cells; Laboratory manuals
Description: This volume has been assembled for scientists interested in basic and applied biomedical research directed toward understanding the development, genetics, and function of cardiomyocytes. The methods and protocols contained within it address cell culture techniques, cardiomyocyte differentiation and redifferentiation, experimental induction of cardiomyopathies, introducing genes into cardiomyocytes, genomic approaches to the understanding cardiomyocytes, cryopreservation of neonatal cardiomyocytes, and modeling of cardiomyocyte function. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and current, Cardiomyocytes: Methods and Protocols explores complimentary areas of cardiomyocyte science that, taken together, can inform individuals with a broad range of interests.--
Notes: Includes bibliographical references and index.
Table of contents: title: Generating primary cultures of murine cardiac myocytes and cardiac fibroblasts to study viral myocarditis  -- Barbara Sherry; title: Enrichment of cardiomyocytes in primary cultures of murine neonatal hearts  -- Sreejit Parameswaran ... [et al.]; title: Deep sequencing of cardiac microRNA-mRNA interactomes in clinical and experimental cardiomyopathy  -- Scot J. Matkovich and Gerald W. Dorn II; title: Next-generation sequencing technology in the genetics of cardiovascular disease  -- Cecilia Vecoli; title: Computational cardiac electrophysiology : implementing mathematical models of cardiomyocytes to Simulate Action Potentials of the Heart  -- Michael M. Bell and Elizabeth M. Cherry; title: Methods of myofibrillogenesis modeling  -- Nancy K. Drew and Anna Grosberg; title: Using the mechanical bidomain model to analyze the biomechanical behavior of cardiomyocytes  -- Bradley J. Roth; title: Fabrication of a myocardial patch with cells differentiated from human-induced pluripotent stem cells  -- Lei Ye, Joydeep Basu, and Jianyi Zhang; title: Efficient differentiation of cardiomyocytes from human pluripotent stem cells with growth factors  -- Rajneesh Jha, Ren-He Xu, and Chunhui Xu; title: Isolation, culturing, and characterization of cardiac muscle cells from nonhuman primate heart tissue  -- Steven M. Hoynowski and John W. Ludlow; title: Mouse embryonic stem cell-derived cardiac myocytes in a cell culture dish  -- Carley Glass ... [et al.]; title: Cryopreservation of neonatal cardiomyocytes  -- Adam C. Vandergriff, M. Taylor Hensley, and Ke Cheng; title: Evaluation of sarcomeric organization in human pluripotent stem cell-derived cardiomyocytes   -- Chrishan J.A. Ramachandra and Winston Shim; title: Electrotonic coupled metabolic purification of chick cardiomyocytes  -- Winston Shim ... [et al.]; title: Gene transfer into cardiac myocytes  -- Sarah E. Lang and Margaret V. Westfall; title: Analysis of 4D myocardial wall motion during early stages of chick heart development -- Madeline Midgett and Sandra Rugonyi.