Disease gene identification
Subtitle: methods and protocols
By: edited by Johanna K. DiStefano
Series: Springer protocols; Methods in molecular biology -- 700; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 700.
Publisher: Humana Press
Published: 2011
Place of publication: New York, NY
Language: eng
Number of pages: 312
ISBN-13: 9781617379536; 9781617379543
Subjects: Genetic Techniques; Genetic Predisposition to Disease; Technique; Medical genetics
Notes: Includes bibliographical references and index.
Table of contents: title: Machine generated contents note: pt. I Introduction; title: 1.Technological Issues and Experimental Design of Gene Association Studies / Darin M. Taverna; title: 2.Statistical Issues in Gene Association Studies / Richard M. Watanabe; title: 3.Identification of Causal Sequence Variants of Disease in the Next Generation Sequencing Era / Christopher B. Kingsley; title: pt. II Methods for Gene Identification; title: 4.Microarray-Based Genome-Wide Association Studies Using Pooled DNA / David W. Craig; title: 5.Medium-Throughput SNP Genotyping Using Mass Spectrometry: Multiplex SNP Genotyping Using the iPLEX® Gold Assay / Meredith P. Millis; title: 6.Targeted SNP Genotyping Using the TaqMan® Assay / Peter Kovacs; title: 7.Bar-Coded, Multiplexed Sequencing of Targeted DNA Regions Using the Illumina Genome Analyzer / David W. Craig; title: pt. III Functional Characterization of Susceptibility Alleles and Loci; title: 8.Site-Directed Mutagenesis / Sukru Tuzmen; title: 9.Gene Expression Profiling of Tissues and Cell Lines: A Dual-Color Microarray Method / Sonsoles Shack; title: 10.Methods for MicroRNA Microarray Profiling / Glen J. Weiss; title: 11.Allelic Expression Profiling to Dissect Genome-Wide Association Study Signals / Jonathan D. Gruber; title: 12.Quantitative Polymerase Chain Reaction Using the Comparative C Method / Kimberly Teatts; title: 13.Genomic Analysis by Oligonucleotide Array Comparative Genomic Hybridization Utilizing Formalin-Fixed, Paraffin-Embedded Tissues / Galen Hostetter; title: 14.RNA Mapping Protocols: Northern Blot and Amplification of cDNA Ends / Mahtab Nourbakhsh; title: 15.High-Content RNA Interference Assay: Analysis of Tau Hyperphosphorylation as a Generic Paradigm / Travis Dunckley; title: pt. IV Alternative Approaches; title: 16.Integrative Systems Biology Approaches to Identify and Prioritize Disease and Drug Candidate Genes / Anil G. Jegga; title: 17.Identification of a Common Variant Affecting Human Episodic Memory Performance Using a Pooled Genome-Wide Association Approach: A Case Study of Disease Gene Identification / Matthew J. Huentelman; title: 18.RNAi-Based Functional Pharmacogenomics / David Azorsa; title: 19.Genetic Predisposition to &beta;-Thalassemia and Sickle Cell Anemia in Turkey: A Molecular Diagnostic Approach / Sukru Tuzmen.