Multiple-mouse magnetic resonance imaging.
Published: 2005
Language: eng
Number of pages: 117
Description: Magnetic resonance imaging (MRI) has a strong role to play in the characterization of genetically altered mice from transgenic and knockout studies, random mutagenesis programs, and the study of traditional mouse models of disease such as mice bearing tumor xenografts. Since the phenotype of a mouse may be unknown, whole-body three-dimensional imaging at a high resolution is needed to detect abnormalities and quantify their size and shape accurately without partial-volume effects. The imaging time per mouse, however, is on the order of hours to produce adequate signal-to-noise ratios (SNRs) in the images. To overcome the inherently low throughput of MRI, this thesis demonstrates a high-field, live multiple-mouse MRI technique that considerably increases the number of mice imaged without either increasing the imaging time or sacrificing SNR.First, the theory of multiple-mouse imaging is developed and a method for producing the highest throughput in an MRI scanner equipped with multiple radiofrequency coils and receiver channels is evaluated on a low-field clinical MRI scanner. Next, that method is implemented on a high-field animal MRI scanner for imaging the brains of seven live mice at once. Finally, the multiple-mouse MRI scanner is used in a phenotyping study of a transgenic mouse line with abnormalities in brain structure, and in a study of a mouse model of brain cancer with a human glioma xenograft.
Notes: Source: Dissertation Abstracts International, Volume: 66-10, Section: B, page: 5275.

Thesis (Ph.D.)--University of Toronto, 2005.

Electronic version licensed for access by U. of T. users.