Bacterial transcriptional control
Subtitle: methods and protocols
By: edited by Irina Artsimovitch, Department of Microbiology, The Ohio State University, Columbus, OH, USA, Thomas J. Santangelo, Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO, USA
Contributors: Springer Science+Business Media
Series: Methods in molecular biology -- 1276; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- 1276,; Springer protocols (Series)
Publisher: Humana Press
Published: 2015
Place of publication: New York
Language: eng
Number of pages: 342
ISBN-13: 9781493923915; 9781493923922
Copyright date: 2015
Subjects: Genetics; Genetic transcription; Laboratory manuals; RNA polymerases; DNA-Directed RNA Polymerases; Genetic Transcription; Laboratory Manuals; Bacterial Proteins
Notes: Includes bibliographical references and index.
Table of contents: title: Mapping the Escherichia coli transcription elongation complex with exonuclease III  -- Zhaokun Liu and Irina Artsimovitch; title: Purification of bacterial RNA polymerase : tools and protocols  -- Vladimir Svetlov and Irina Artsimovitch; title: Monitoring translocation of multisubunit RNA polymerase along the DNA with fluorescent base analogues  -- Anssi M. Malinen, Matti Turtola, and Georgiy A. Belogurov; title: In vitro and in vivo methodologies for studying the sigma-54-dependent transcription  -- Martin Buck [and nine others]; title: Methods for the assembly and analysis of in vitro transcription-coupled-to-translation systems  -- Daniel Castro-Roa and Nikolay Zenkin; title: Site-specific incorporation of probes into RNA polymerase by unnatural-amino-acid mutagenesis and Straudinger-Bertozzi ligation  -- Anirban Chakraborty [and seven others]; title: Reconstruction of factor-dependent, promotor proximal pausing in Drosophila nuclear extracts  -- Jian Li and David S. Gilmour; title: Direct competition assay for transcription fidelity  -- Lucyna Lubkowska and Maria L. Kireeva; title: Single-stranded DNA aptamers for functional probing of bacterial RNA polymerase  -- Danil Pupov and Andrey Kulbachinskiy; title: Biochemical analysis of transcription termination by RNA polymerase III from yeast Saccharomyces cerevisiae  -- Aneeshkumar G. Arimbasseri and Richard J. Maraia; title: Use of RNA polymerase molecular beacon assay to measure RNA polymerase interactions with model promotor fragments  -- Vladimir Mekler and Konstantin Severinov; title: Preparation of cDNA libraries for high-throughput RNA sequencing analysis of RNA 5' ends  -- Irina O. Vvedenskaya, Seth R. Goldman, and Bryce E. Nickels; title: In situ footprinting of E. coli transcription elongation complex with chloroacetaldehyde  -- A. Rachid Rahmouni and Christine Mosrin-Hauman; title: Using solutes and kinetics to probe large conformational changes in the steps of transcription initiation  -- Emily F. Ruff, Wayne S. Kontur, and M. Thomas Record Jr.; title: Manipulating archaeal systems to permit analyses of transcription elongation-termination decisions in vitro  -- Alexandra M. Gehring and Thomas J. Santangelo; title: Purification of active RNA polymerase I from yeast  -- Francis Dean Appling and David Alan Schneider; title: Transcription in archaea : preparation of Methanocaldococcus jannaschii transcription machinery  -- Katherine Smollet, Fabian Blombach, and Finn Werner; title: Transcription in archaea ; in vitro transcription assays for mjRNAP  -- Katherine Smollet, Fabian Blombach, and Finn Werner; title: Experimental analysis of hFACT action during pol II transcription in vitro  -- Fu-Kai Hsieh, Olga I. Kulaeva, and Vasily M. Studitsky; title: ChIP-Seq for genome-scale analysis of bacterial DNA-binding proteins -- Richard P. Bonocora and Joseph T. Wade.