Signal transduction immunohistochemistry
Subtitle: methods and protocols
By: edited by Alexander E. Kalyuzhny
Series: Springer protocols; Methods in molecular biology -- 717; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 717.
Publisher: Humana
Published: 2011
Place of publication: New York, NY
Language: eng
Number of pages: 302
ISBN-13: 9781617790232; 9781617790249
Subjects: Immunohistochemistry; Signal Transduction; Cellular signal transduction
Notes: Includes bibliographical references and index.
Table of contents: title: Overview of the generation, validation, and application of phosphosite-specific antibodies  -- Kathy Brumbaugh ... [et al.]; title: Selection and validation of antibodies for signal transduction immunohistochemistry  -- Juraj Bodo and Eric D. Hsi; title: Overview of western blotting for determining antibody specificities for immunohistochemistry  -- Biji T. Kurien; title: Optimized protocol to make phospho-specific antibodies that work  -- Amy J. Archuleta ... [et al.]; title: Methodology and technology for stabilization of specific states of signal transduction proteins  -- Mats Boren; title: Enhanced antigen-retrieval protocol for immunohistochemical staining of formalin-fixed, paraffin-embedded tissues  -- Sergei I. Syrbu and Michael B. Cohen; title: Imaging techniques in signal transduction IHC  -- Jerry Sedgewick; title: Practical considerations of image analysis and quantification of signal transduction IHC staining  -- Michael Grunkin, Jakob Raundahl, and Niels T. Foged; title: Flow cytometric analysis of cell signaling proteins  -- Maria A. Suni and Vernon C. Maino; title: CytoSys : a tool for extracting cell-cycle-related expression dynamics from static data  -- Jayant Avva ... [et al.]; title: Signaling events initiated by kappa opioid receptor activation : quantification and immunocolocalization using phospho-selective KOR, p38 MAPK, and KIR 3.1 antibodies  -- Julia C. Lemos, Clarisse A. Roth, and Charles Chavkin; title: Immunohistochemical assessment of signal transduction and cell-cycle networks in neural tumors  -- Daniel Ciznadija, Afsar Barlas, and Katia Manova; title: Novel multicolor immunofluorescence technique using primary antibodies raised in the same host species  -- Jillian Frisch ... [et al.]; title: Activation and differentiation of mesenchymal stem cells  -- Pravin J. Mishra and Debabrata Banerjee; title: Double in situ detection of sonic hedgehog mRNA and pMAPK protein in examining the cell proliferation signaling pathway in mouse embryo  -- Sho Fujisawa ... [et al.]; title: Identifying intracellular sites of eicosanoid lipid mediator synthesis with eicosacell assays  -- Christianne Bandeira-Melo, Peter F. Weller, and Patricia T. Bozza; title: Absorption control in immunohistochemistry using phospho-peptides immobilized on magnetic beads -- Jordan Schoephoerster ... [et al.].