Flower development
Subtitle: methods and protocols
By: edited by José Luis Riechmann, Frank Wellmer
Series: Methods in molecular biology -- 1110; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 1110.; Springer protocols (Series)
Publisher: Humana Press; Springer
Published: 2014
Place of publication: New York
Language: eng
Number of pages: 475
ISBN-13: 9781461494072; 9781461494089
Subjects: Flowers; Laboratory manuals; Laboratory Manuals; Growth & development; Angiosperms; Genetics
Description: In Flower Development: Methods and Protocols, researchers in the field detail protocols for experimental approaches that are currently used to study the formation of flowers, from genetic methods and phenotypic analyses, to genome-wide experiments, modeling, and system-wide approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Flower Development: Methods and Protocols is an essential guide for plant developmental biologists, from the novice to the experienced researcher, and for those considering venturing into the field --
Notes: Includes bibliographical references and index.
Table of contents: title: Flower Development in Arabidopsis; title: There is More to it than Learning your ABCs; title: Flower Development in the Asterid Lineage; title: Grass Flower Development; title: Flower Diversity and Angiosperm Diversification; title: Flower Development; title: Open Questions and Future Directions; title: Genetic Screens for Floral Mutants in Arabidopsis thaliana: Enhancers and Suppressors; title: Genetic and Phenotypic Analysis of Shoot Apical and Floral Meristem Development; title: Genetic and Phenotypic Analyses of Petal Development in Arabidopsis; title: Cell Biological Analyses of Anther Morphogenesis and Pollen Viability in Arabidopsis and Rice; title: Molecular Cell Biology of Male Meiotic Chromosomes and Isolation of Male Meiocytes in Arabidopsis thaliana; title: Genetic and Phenotypic Analyses of Carpel Development in Arabidopsis; title: Microscopic Analysis of Arabidopsis Ovules; title: Scanning Electron Microscopy Analysis of Floral Development; title: Detection of mRNA Expression Patterns by Non-radioactive in situ Hybridization on Histological Sections of Floral Tissue; title: The GUS Reporter System in Flower Development Studies; title: A Floral Induction System for the Study of Early Arabidopsis Flower Development; title: Fluorescence Activated Cell Sorting of Shoot Apical Meristem Cell Types; title: Translating Ribosome Affinity Purification (TRAP) for Cell-specific Translation Profiling in Developing Flowers; title: Laser-assisted Microdissection Applied to Floral Tissues; title: Identification of Arabidopsis Knock-out Lines for Genes of Interest; title: Gene Expression Analysis by Quantitative Real-time PCR for Floral Tissues; title: Misexpression Approaches for the Manipulation of Flower Development; title: Next Generation Sequencing Applied to Flower Development: RNA-Seq; title: Next-Generation Sequencing Applied to Flower Development: ChIP-Seq; title: Live-Imaging of the Arabidopsis Inflorescence Meristem; title: Gene Regulatory Network Models for Floral Organ Determination.