E. coli Gene Expression Protocols
Edition: 1st edition
Publisher: Humana Press
Published: October 15, 2002
Language: eng
Format: Hardcover
Number of pages: 368
ISBN-13: 9781588290083
Subjects: Molecular biology; Science/Mathematics; Life Sciences - Biochemistry; Bacterial Genetics; Microbial Genetics; Science; Life Sciences - Biology - Microbiology; Life Sciences - Genetics & Genomics; Science / Cytology; Gene expression; Life Sciences - Bacteriology; Escherichia coli; Genetics; Laboratory manuals
First sentence: Rapid transfer of exponentially growing E. coli cultures from physiological to low temperatures (10-15 C) has profound consequences on cell physiology: membrane fluidity decreases, which interferes with transport and secretion, the secondary structures of nucleic acids are stabilized, which affect the efficiencies of mRNA transcription/translation and DNA replication, and free ribosomal sub-units and 70S particles accumulate at the expense of polysomes, negatively impacting translation of most cellular mRNAs (1-3).
Notes: Methods in Molecular Biology