Human embryonic stem cell protocols
By: edited by Kursad Turksen.
Contributors: Turksen, Kursad.
Series: Methods in molecular biology,; v. 331; Methods in molecular biology (Clifton, N.J.) ;; v. 331.
Publisher: Humana Press
Published: 2006
Place of publication: Totowa, N.J
Language: eng
Number of pages: 385
Subjects: Embryonic stem cells -- Laboratory manuals; Human embryo -- Laboratory manuals; Stem Cells; Embryo Research
Genres: Laboratory manuals.
Description: A comprehensive collection of diverse techniques for the molecular and cellular manipulation of human embryonic stem (hES) cells. These readily reproducible methods have been optimized for the derivation, characterization, and differentiation of hES cells, with special attention given to regenerative medicine applications. A companion CD provides color versions of all illustrations in the book.
Notes: Includes bibliographical references and index.
Table of contents: title: Human embryonic stem cells and differentiation / Kursad Turksen; title: Blastocyst culture for deriving human embryonic stem cells / Ariff Bongso; title: Establishment of a pluripotent human embryonic stem cell line from a cloned blastocyst / Woo Suk Hwang, Byeong Chun Lee, and Sung Keun Kang; title: Propagation of human embryonic stem cells on human feeder cells / Mark Richards and Ariff Bongso; title: Derivation and maintenance of human embryonic stem cells / Michal Amit and Joseph Itskovitz-Eldor; title: Isolation and characterization of human embryonic stem cells / Maisam Mitalipova and Gail Palmarini; title: Routine culture and differentiation of human embryonic stem cells / Jim McWhir, Davina Wojtacha, and Alison Thomson; title: Maintenance of human embryonic stem cells in animal-serum- and feeder-layer- free culture conditions cells on human feeder layers / Michal Amit and Joseph Itskovitz-Eldor; title: Manipulation of self-renewal in human embryonic stem cells through a novel pharmacological GSK-3 inhibitor / Ali H. Brivanlou; title: Derivation of human feeders for prolonged support of human embryonic stem cells / Chui-Yee Fong and Ariff Bongso; title: Transplantation of human embryonic stem cells to the chick embryo / Ronald S. Goldstein; title: Derivation and characterization of neuronal precursors and dopaminergic neurons from human embryonic stem cells in vitro / Melissa Carpenter ... [et al.]; title: In vitro differentiation of neural precursors from human embryonic stem cells / Xue-Jun Li and Su-Chun Zhang; title: Derivation and characterization of hematopoietic cells from human embryonic stem cells / Lisheng Wang ... [et al.]; title: Retroviral transduction of hematopoietic progenitors derived from human embryonic stem cells / Pablo Menendez ... [et al.]; title: Genetic manipulation of human embryonic stem cells by transfection / Rachel Eiges; title: Designing, testing, and validating microarray for characterization of stem cells / Yongquan Luo ... [et al.]; title: Confrontation cultures of embryonic stem cells with multicellular tumor spheroids to study tumor-induced angiogenesis / Maria Wartenberg ... [et al.]; title: A novel experimental platform for investigating cancer growth and anti-cancer therapy in a human tissue micro-environment derived from human embryonic stem cells / Maty Tzukerman and Karl Skorecki; title: The analysis of mitochondria and mitochondrial DNA in human embryonic stem cells / Justin C. St. John ... [et al.]; title: Microarray approach to identify the signaling network responsible for self-renewal of human embryonic stem cells / Ali H. Brivanlou; title: Massively parallel signature sequencing / Daixing Zhou ... [et al.].