Cell imaging techniques
Subtitle: methods and protocols
By: edited by Douglas J. Taatjes, Brooke T. Mossman
Contributors: Taatjes, Douglas J; Mossman, Brooke T., 1947-
Series: Methods in molecular biology -- 319; Methods in molecular biology (Clifton, N.J.) -- 319
Publisher: Humana Press
Published: 2006
Place of publication: Totowa, N.J
Language: eng
Number of pages: 490
Subjects: Cytology; Microscopy; Molecular biology; Imaging systems in biology
Description: This book presents state-of-the-art chapters on a wide variety of microscopy-based imaging techniques. It covers a wide range of complimentary techniques, including methods such as fluorescence microscopy, electron microscopy, atomic force microscopy, and laser scanning cytometry, to name a few. Cell imaging techniques are now used in a variety of disciplines, including cell biology, pathology, physiology, pharmacology, molecular biology, neurobiology, biophysics and genetics. This book will prompt researchers to consider using a variety of complimentary imaging techniques to address their specific research questions, as opposed to the previously limited selection available.
Notes: Includes bibliographical references and index
Table of contents: title: Molecular beacons: fluorescent probes for detection of endogenous mRNAs in living cells; title: Second-harmonic imaging of collagen; title: Visualizing calcium signaling in cells by digitized wide-field and confocal fluorescent microscopy; title: Multifluorescence labeling techniques and confocal laser scanning microscopy on lung tissue; title: Evaluation of confocal microscopy system performance; title: Quantitative analysis of atherosclerotic lesion composition in mice; title: Applications of microscopy to genetic therapy of cystic fibrosis and other human diseases; title: Laser scanning cytometry: principles and applications; title: Near-clinical applications of laser scanning cytometry; title: Laser capture microdissection; title: Analysis of asbestos-induced gene expression changes in bronchiolar epithelial cells using laser capture microdissection and quantitative reverse transriptase-polymerase chain reaction; title: New approaches to fluorescence in situ hybridization; title: Microarray image scanning; title: Near-field scanning optical microscopy in cell biology and cytogenetics; title: Porosome: the fusion pore revealed by multiple imaging modalities; title: Secretory vesicle swelling by atomic force microscopy; title: Imaging and probing cell mechanical properties with the atomic force microscopy; title: Reflection contrast microscopy: the bridge between light and electron microscopy; title: Three-dimensional analysis of single particles by electron microscopy: sample preparation and data acquisition; title: Three-dimensional reconstruction of single particles in electron microscopy: image processing; title: A new microbiopsy system enables rapid preparation of tissue for high-pressure freezing