Ion channels
Subtitle: methods and protocols
By: edited by James D. Stockand, Mark S. Shapiro.
Contributors: Stockand, James D.; Shapiro, Mark S.
Series: Methods in molecular biology,; v. 337; Methods in molecular biology (Clifton, N.J.) ;; v. 337.
Publisher: Humana Press
Published: 2006
Place of publication: Totowa, N.J
Language: eng
Number of pages: 212
Subjects: Ion channels; Ion Channels
Description: Reviews practical implementation of the study of ion channels and should be used as a field guide by the investigator when designing and performing research on channels. Each chapter provides details about a particular aspect of investigating ion channels. The detail includes description of the actual successfully employed experimental procedures and pitfalls for each application.
Notes: Includes bibliographical references and index.
Table of contents: title: Functional reconstitution of the human epithelial Na+ channel in a mammalian expression system / Alexander Staruschenko ... [et al.]; title: Overexpression of proteins in neurons using replication-deficient virus / Richard M. Ahlquist and Jane M. Sullivan; title: Exogenous expression of proteins in neurons using the biolistic particle delivery system / Nikita Gamper and Mark S. Shapiro; title: Tertiary and quaternary structure formation of voltage-gated potassium channels / John M. Robinson, Andrey Kosolapov, and Carol Deutsch; title: Biophysical approach to determine the subunit stoichiometry of the epithelial sodium channel using the xenopus laevis oocyte expression system / Farhad Kosari, Shaou Sheng, and Thomas R. Kleyman; title: Spectroscopy-based quantitative fluorescence resonance energy transfer analysis / Jie Zheng; title: Probing the effects of phosphoinositides on ion channels / Chou-Long Huang; title: Epithelial sodium channel in planar lipid bilayers / Bakhrom K. Berdiev and Dale J. Benos; title: A simple in-vivo method for assessing changes of membrane bound ion channel density in xenopus oocytes / Mouhamed S. Awayda ... [et al.]; title: Preparation of cortical brain slices for electrophysiological recording / Costa M. Colbert; title: Juxtacellular labeling and chemical phenotyping of extracellulary recorded neurons in vivo / Glenn M. Toney and Lynette C. Daws; title: Carbon fiber amperometry in the study of ion channels and secretion / Duk-Su Koh; title: Genetic screening for functionality of bacterial potassium channel mutants using K+ update-deficient escherichia coli / Lyubov V. Parfenova and Brad S. Rothberg; title: KCNQ1 K channel-mediated cardiac channelopathies / Gildas Loussouarn, Isabelle Baro, and Denis Escade; title: Tissue-specific transgenic and knockout mice / Andree Porret ... [et al.].