Vertebrate embryogenesis
Subtitle: embryological, cellular, and genetic methods
By: edited by Francisco J. Pelegri
Series: Methods in molecular biology -- 770; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 770.; Springer protocols
Publisher: Humana Press; Springer
Published: 2011
Place of publication: New York
Language: eng
Number of pages: 605
ISBN-13: 9781617792090; 9781617792106
Subjects: Experimental Embryology; Vertebrates; Embryology; Genetics; Embryonic Development
Description: [Publisher-supplied data] One of the striking findings of modern developmental biology has been the high degree of conservation of signaling and developmental mechanisms amongst different animal species. Such conservation allows information learned from a given organism to be applicable to other species, including humans, and has validated the use of a few model systems to deduce general biological principles. In spite of this underlying conservation, however, each species has unique characteristics arising from its evolutionary history. Vertebrate Embryogenesis: Embryological, Cellular and Genetic Methods attempts to address the increasingly important need of straddling species boundaries in the context of a single research program by compiling research protocols used in a wide range of vertebrate species. In fact, this volume has been designed so that readers can readily find information on species other than the one with which they may be most familiar.

These protocols include not only embryological methods, but also cellular and genetic approaches that have complemented and expanded our understanding of embryonic development. In addition, a number of chapters highlight a specific method that is in principle applicable to multiple species, such as TILLING and ZFN-mediated mutagenesis, the generation of Embryonic Stem (ES) cell lines, and nuclear/oocyte transfer. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and accessible, Vertebrate Embryogenesis: Embryological, Cellular and Genetic Methods serves as an ideal guide to the molecular, cell, and developmental biology community and will hopefully contribute to the ongoing collective effort towards a better understanding of the beauty and logic of vertebrate development.
Notes: Includes bibliographical references and index.
Table of contents: title: Approaches and species in the history of vertebrate embryology  -- Nick Hopwood; title: Manipulating and imaging the early Xenopus laevis embryo  -- Michael V. Danilchik; title: Manipulation of gene function in Xenopus laevis  -- Mizuho S. Mimoto and Jan L. Christian; title: Developmental genetics in Xenopus tropicalis  -- Timothy J. Geach and Lyle B. Zimmerman; title: Embryological and genetic manipulation of chick development  -- Laura S. Gammill and Catherine E. Krull; title: Embryological manipulations in zebrafish  -- Yuhua Sun, Dorota Wloga, and Scott T. Dougan; title: Practical approaches for implementing forward genetic strategies in zebrafish  -- Sreelaja Nair and Francisco J. Pelegri; title: Essential techniques for introducing medaka to a zebrafish laboratory - towards the combined use of medaka and zebrafish for further genetic dissection of the function of the vertebrate genome  -- Sean R. Porazinski, Huijia Wang, and Makoto Furutani-Seiki; title: Ex utero culture and live imaging of mouse embryos  -- Anna Piliszek, Gloria S. Kwon, and Anna-Katerina Hadjantonakis; title: Detection of gene expression in mouse embryos and tissue sections  -- Edwina McGlinn and Jennifer H. Mansfield; title: Gene targeting in the mouse  -- Anne E. Griep ... [et al.]; title: Creating a "hopeful monster" : mouse forward genetic screens  -- Vanessa L. Horner and Tamara Caspary; title: Assisted reproductive technology in nonhuman primates  -- Tien-cheng Arthur Chang and Anthony W.S. Chan; title: Embryological methods in ascidians : the Villefranche-sur-Mer protocols  -- Christian Sardet ... [et al.]; title: Ciona genetics  -- Michael T. Veeman, Shota Chiba, and William C. Smith; title: Analyses of gene function in Amphioxus embryos by microinjection of mRNAs and morpholino oligonucleotides   -- Linda Z. Holland and Takayuki Onai; title: Reptile embryology  -- Matthew K. Vickaryous and Katherine E. McLean; title: Reproductive and developmental manipulation of the marsupial, the tammar wallaby Macropus eugenii  -- Marilyn B. Renfree and Andrew J. Pask; title: Mutant generation in vertebrate model organisms by TILLING  -- Sylke Winkler, Nicola Gscheidel, and Michael Brand; title: Inducing high rates of targeted mutagenesis in zebrafish using zinc finger nucleases (ZFNs)  -- Jasmine M. McCammon, Yannick Doyon, and Sharon L. Amacher; title: Derivation of mouse embryonic stem cell lines from blastocysts produced by fertilization and somatic cell nuclear transfer  -- Zhongde Wang; title: Cloning mice and ES cells by nuclear transfer from somatic stem cells and fully differentiated cells  -- Zhongde Wang; title: Keeping two animal systems in one lab - a frog plus fish case study  -- Hazel Sive; title: Laboratory guidelines for animal care -- Marcelo Couto.