Polyadenylation in plants
Subtitle: methods and protocols
By: edited by Arthur G. Hunt, Department of Plant and Soil Sciences, University of Kentucky, KY, USA, Qingshun Quinn Li, Department of Biology, Miami University, OH, USA, Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, College of the Environment and Ecology, Xiamen University, Xiamen, Fujian, China
Series: Methods in molecular biology -- 1255; Springer protocols; Methods in molecular biology (Clifton, N.J.) -- v. 1255.; Springer protocols (Series)
Publisher: Humana Press
Published: 2015
Place of publication: New York
Language: eng
Number of pages: 225
ISBN-13: 9781493921744; 9781493921751
Subjects: Plants; Plant gene expression; Laboratory Manuals; Genetics; Polyadenylation; Laboratory manuals
Notes: Includes bibliographical references (some color) and index.
Table of contents: title: Computational analysis of plant polyadenylation signals / Xiaohui Wu , Guoli Ji , and Qingshun Quinn Li; title: Prediction of plant mRNA polyadenylation sites / Xiaohui Wu , Guoli Ji , and Qingshun Quinn Li; title: Extraction of poly(A) sites from large-scale RNA-seq data / Min Dong ... [et al.]; title: Poly(A)-tag deep sequencing data processing to extract poly(A) sites / Xiaohui Wu, Guoli Ji, and Qingshun Quinn Li; title: Analysis of poly(A) site choice using a java-based clustering algorithm / Patrick E. Thomas; title: RADPRE : a computational program for identification of differential mRNA processing including alternative polyadenylation / Denghui Xing and Qingshun Quinn Li; title: Characterization of plant polyadenylation complexes by using tandem affinity purification / Hongwei Zhao, Xinfu Ye, and Qingshun Quinn Li; title: In vitro analysis of cleavage and polyadenylation in Arabidopsis  / Hongwei Zhao and Qingshun Quinn Li; title: Production, purification, and assay of recombinant proteins for in vitro biochemical analyses of the plant polyadenylation complex / Stephen A. Bell and Balasubrahmanyam Addepalli; title: Detection of disulfide linkage by chemical derivatization and mass spectrometry / Balasubrahmanyam Addepalli; title: Transient expression using agroinfiltration to study polyadenylation in plants / Carol Von Lanken and Arthur G. Hunt; title: 3' RACE protocol to confirm polyadenylation sites / Liuyin Ma and Arthur G. Hunt; title: Phage display library screening for identification of interacting protein partners / Balasubrahmanyam Addepalli, Suryadevara Rao, and Arthur G. Hunt; title: Genome- wide determination of poly(A) site choice in plants / Pratap Kumar Pati, Liuyin Ma, and Arthur G. Hunt; title: DNA/RNA hybrid primer mediated poly(A) tag library construction for illumina sequencing / Man Liu, Xiaohui Wu, and Qingshun Quinn Li; title: Poly(A) tag library construction from 10 ng total RNA / Jingyi Cao and Qingshun Quinn Li; title: Rapid, simple, and inexpensive method for the preparation of strand-specific RNA-seq libraries / Arthur G. Hunt; title: Genome-wide analysis of distribution of RNA polymerase II isoforms using ChIP-Seq / Laura de Lorenzo.